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The Translational Regulation Of Lipoprotein Lipase By Epinephrine Involves An Rna Binding Complex Including The Catalytic Subunit Of Protein Kinase A, Gouri Ranganathan, Dan Phan, Irina D. Pokrovskaya, Joan E. Mcewen, Chunling Li, Philip A. Kern

Clinical and Translational Science Faculty Publications

The balance of lipid flux in adipocytes is controlled by the opposing actions of lipolysis and lipogenesis, which are controlled primarily by hormone-sensitive lipase and lipoprotein lipase (LPL), respectively. Catecholamines stimulate adipocyte lipolysis through reversible phosphorylation of hormone-sensitive lipase, and simultaneously inhibit LPL activity. However, LPL regulation is complex and previous studies have described translational regulation of LPL in response to catecholamines because of an RNA-binding protein that interacts with the 3′-untranslated region of LPL mRNA. In this study, we identified several protein components of an LPL RNA binding complex. Using an LPL RNA affinity column, we identified two of …

Endogenous Gonadal Hormone Exposure And Bone Sarcoma Risk, Dawn M. Cooley, Benjamin C. Beranek, Deborah L. Schlittler, Nita W. Glickman, Lawrence T. Glickman, David J. Waters

Endocrinology Collection

Although experimental and clinical evidence suggest that endogenous sex hormones influence bone sarcoma genesis, the hypothesis has not been adequately tested in an appropriate animal model. We conducted a historical cohort study of Rottweiler dogs because they frequently undergo elective gonadectomy and spontaneously develop appendicular bone sarcomas, which mimic the biological behavior of the osteosarcomas that affect children and adolescents. Data were collected by questionnaire from owners of 683 Rottweiler dogs living in North America. To determine whether there was an association between endogenous sex hormones and risk of bone sarcoma, relative risk (RR) of incidence rates and hazard ratios …

Flight Physician - November, 2002, Civil Aviation Medical Association

Browse all Civil Aviation Medical Association Newsletters

A sixteen page newsletter of the Civil Aviation Medical Association. The newsletter provided news about civil aviation medicine and information related to the organization.

Oncolog, Volume 47, Number 11, November 2002, Mariann Crapanzano, Kerry L. Wright, Frank A. Morello Jr. Md

OncoLog MD Anderson's Report to Physicians (All issues) Archives

• Diagnosis Based on Nonsurgical Biopsy: A Cooperative Effort
• Benign Care: Young Patients with a Variety of Noncancerous Conditions Find Treatment at M.D. Anderson Cancer Center
• Cognitive Interventions Are Crucial for Many Patients with Neurofibromatosis
• FNA Clinic Streamlines Biopsy Diagnosis of Palpable Lesions
• "Is This Going to Hurt?", by Frank A. Morello Jr., MD, Assistant Professor, Section of Vascular and Interventional Radiology
• House Call: Answers to Common Questions about the Use of Radioisotopes for Diagnosis

Individual, Temporal, And Seasonal Variation In Sperm Concentration In Tree Swallows, Michael P. Lombardo, Armetris N. Forman, Matthew R. Czarnowski, Patrick A. Thorpe

Peer Reviewed Publications

We determined sperm concentrations in Tree Swallows (Tachycineta bicolor) by manually expressing semen samples from males during prelaying, egg-laying, incubation, and nestling periods. Sperm concentrations varied by orders of magnitude (0-10⁹ sperm mL^-1) among males. Sperm concentrations were highest during the incubation period and lowest during the prelaying period. None of the samples collected during the prelaying, egg-laying, and incubation periods were devoid of sperm. In contrast, 45% of samples collected during the nestling period lacked sperm. Sperm concentrations (1) did not vary over the course of the morning during prelaying, egg-laying, and incubation periods …

Associations Between Cardiorespiratory Fitness And C-Reactive Protein In Men, Timothy S. Church, Carolyn E. Barlow, Conrad P. Earnest, James B. Kampert, Elisa L. Priest, Steven N. Blair

Faculty Publications

Objective - This study examined the association between cardiorespiratory fitness and C-reactive protein (CRP), with adjustment for weight and within weight categories.

Methods and Results - We calculated median and adjusted geometric mean CRP levels, percentages of individuals with an elevated CRP (≥2.00 mg/L), and odds ratios of elevated CRP across 5 levels of cardiorespiratory fitness for 722 men. CRP values were adjusted for age, body mass index, vitamin use, statin medication use, aspirin use, the presence of inflammatory disease, cardiovascular disease, and diabetes, and smoking habit. We found an inverse association of CRP across fitness levels (P for …

Medical Students' Personality Characteristics And Academic Performance: A Five-Factor Model Perspective, Filip Lievens, Pol Coetsier, Filip De Fruyt, Jan De Maeseneer

Research Collection Lee Kong Chian School Of Business

Objectives: This study investigates: (1) which personality traits are typical of medical students as compared to other students, and (2) which personality traits predict medical student performance in pre-clinical years. Design: This paper reports a cross-sectional inventory study of students in nine academic majors and a prospective longitudinal study of one cohort of medical students assessed by inventory during their first pre-clinical year and by university examination at the end of each pre-clinical year. Subjects and methods: In 1997, a combined total of 785 students entered medical studies courses in five Flemish universities. Of these, 631 (80.4%) completed the NEO-PI-R …

A Reduced Model Clarifies The Role Of Feedback Loops And Time Delays In The Drosophila Circadian Oscillator, Paul Smolen, Douglas A. Baxter, John H. Byrne

Faculty, Staff and Student Publications

Although several detailed models of molecular processes essential for circadian oscillations have been developed, their complexity makes intuitive understanding of the oscillation mechanism difficult. The goal of the present study was to reduce a previously developed, detailed model to a minimal representation of the transcriptional regulation essential for circadian rhythmicity in Drosophila. The reduced model contains only two differential equations, each with time delays. A negative feedback loop is included, in which PER protein represses per transcription by binding the dCLOCK transcription factor. A positive feedback loop is also included, in which dCLOCK indirectly enhances its own formation. The model …

Toxoplasma Gondii Induces Granulocyte Colony-Stimulating Factor And Granulocyte-Macrophage Colony-Stimulating Factor Secretion By Human Fibroblasts: Implications For Neutrophil Apoptosis, Jacqueline Y. Channon, Kristin A. Miselis, Laurie A. Minns, Chaitali Dutta, Lloyd H. Kasper

Dartmouth Scholarship

Human neutrophils are rescued from apoptosis following incubation with once-washed, fibroblast-derived Toxoplasma gondii tachyzoites. Both infected and uninfected neutrophils are rescued, implicating a soluble mediator. In this study we investigated the origin and identity of this soluble mediator. Neutrophils were incubated either with purified tachyzoites or with conditioned medium derived from T. gondii-infected human fibroblasts. Conditioned medium was found to be a potent stimulus that delayed neutrophil apoptosis up to 72 h, whereas purified and extensively washed tachyzoites had no effect. Delayed apoptosis correlated with up-regulation of the neutrophil antiapoptotic protein, Mcl-1, and the neutrophil interleukin 3 receptor alpha subunit …

Altered Expression Of Skeletal Muscle Myosin Isoforms In Cancer Cachexia, Gary M. Diffee, Katherine Kalfas, Sadeeka Al-Majid, Donna O. Mccarthy

College of Nursing Faculty Research and Publications

Cachexia is commonly seen in cancer and is characterized by severe muscle wasting, but little is known about the effect of cancer cachexia on expression of contractile protein isoforms such as myosin. Other causes of muscle atrophy shift expression of myosin isoforms toward increased fast (type II) isoform expression. We injected mice with murine C-26 adenocarcinoma cells, a tumor cell line that has been shown to cause muscle wasting. Mice were killed 21 days after tumor injection, and hindlimb muscles were removed. Myosin heavy chain (MHC) and myosin light chain (MLC) content was determined in muscle homogenates by SDS-PAGE. Body …

Research News: 2002, No. 3, University Of Mississippi. School Of Pharmacy

Research News: Grants and Publications (2000-2014)

Grants, publications, technology transfer, special announcements

The Influences Of Culture, Socioeconomics, And Migration On The Mexican Immigrant Experience, Jean M. Gunderson

Theses and Graduate Projects

During the past eight years, there has been an increase in the migratory patterns offtamilies from Mexico to a rural community in Minnesota, served by Maternal Child public health nurses. Many of these families are undocumented or are of Mixed Citizenship status, citizens and undocumented residing together, and live in poverty. This ethnographic study examines the migratory experiences of these immigrants and the interconnection to the socioeconomic and cultural influences of community.

The primary tools of poetry, cultural stories, and photography, are incorporated in this field project. Understanding the cultural and socioeconomic context of migration for Mexican immigrants may strengthen …

Methods Of Making Modified Polypeptides, Joseph Chappell, Kathleen R. Manna, Joseph P. Noel, Courtney M. Starks

Pharmaceutical Sciences Faculty Patents

Novel polypeptides and the corresponding nucleic acids encoding such polypeptides are disclosed herein. The invention provides methods of making modified polypeptides by altering one or more amino acid residues involved in the active site of a preselected polypeptide.

Topical Micronutrient Delivery System And Uses Of Thereof, Elaine L. Jacobson, Myron Jacobson, Jaber G. Qasem, Hyuntae Kim, Moonsun Kim

Graduate Center for Nutritional Sciences Faculty Patents

The invention involves methods and compositions useful in delivering micronutrients to cells. By formulating the micronutrient in the form of an ester that is convertible to the active form of the micronutrient, one can combine it with a co-ester that inhibits esterases, so that the micronutrient can reach the targeted cells prior to degradation. Both methods and compositions are described.

Regulation Of Lipoprotein Lipase By Protein Kinase Cα In 3t3-F442a Adipocytes, Gouri Ranganathan, Wei Song, Nicholas Dean, Brett Monia, Steven W. Barger, Philip A. Kern

Clinical and Translational Science Faculty Publications

Lipoprotein lipase (LPL) is an important enzyme in adipocyte and lipid metabolism with complex cellular regulation. Previous studies demonstrated an inhibition of LPL activity and synthesis following depletion of protein kinase C (PKC) isoforms with long term treatment of 3T3-F442A adipocytes with 12-O-tetradecanoylphorbol-13-acetate. To identify the specific PKC isoforms involved, we treated cells with antisense oligonucleotides that block expression of specific PKC isoforms. An antisense oligonucleotide to PKCα inhibited LPL activity by 78 ± 8%, whereas antisense oligonucleotides directed against PKCδ or PKCε had no effect on LPL activity. The change in LPL activity was maximal at 72 …

Ada News - 10/07/2002, American Dental Association, Publishing Division

ADA News

Established in 1970 as the official newspaper of the American Dental Association, the ADA News serves practicing dentists and others allied to the dental profession in the U.S. and internationally. It is the No. 1 source of news and information about the many benefits and services the ADA delivers to members daily as well as timely information on scientific, social, political and economic developments affecting dentistry and health care.

Com Outlook Fall 2002, College Of Osteopathic Medicine

COM Outlook

No abstract provided.

Dentalgram - Vol. 16, No. 10, Loma Linda University School Of Dentistry

Dentalgram

In this issue:

• Second Annual Celebration
• Brian Preston selected for ESP 2002-2003
• Orientation for new students
• School of Dentistry Fall Family Retreat
• Dr. Robert L. Frazer Jr., returns for Strategic Planning Retreat
• IDP graduation dinner
• Department news

Laboratory Response To Anthrax Bioterrorism, New York City, 2001, Michael B. Heller, Michel L. Bunning, Martin E.B. France, Debra M. Niemeyer, Leonard Peruski, Tim Naimi, Phillip M. Talboy, Patrick H. Murray, Harald W. Pietz, John Kornblum, William Oleszko, Sara T. Beatrice, Joint Microbiological Rapid Response Team, New York City Anthrax Investigation Working Group, Denis Nash

Publications and Research

In October 2001, the greater New York City Metropolitan Area was the scene of a bioterrorism attack. The scale of the public response to this attack was not foreseen and threatened to overwhelm the Bioterrorism Response Laboratory’s (BTRL) ability to process and test environmental samples. In a joint effort with the Centers for Disease Control and Prevention and the cooperation of the Department of Defense, a massive effort was launched to maintain and sustain the laboratory response and return test results in a timely fashion. This effort was largely successful. The development and expansion of the facility are described, as …

The Influence Of Warning Label Presentation In Memory Recognition Of Aging Adults, Brian J. Call

Master's Theses - Daytona Beach

Research indicates that hundreds of thousands of people are injured each year as a result of poor communication between medical personnel, warning label information, and consumers when taking over the counter or prescription medication. Typically, as adults age, they are increasingly responsible for remembering the hazards of taking medications. If inadequate information exists in memory in regards to side effects, dosage, and other warnings, the likelihood of improper usage will increase. Because aging adults typically have a reduction in cognitive resources, it was hypothesized that older adults would require the aid of additional warning information to assist in the retrieval …

Development And Evaluation Of The Sleep Treatment And Education Program For Students (Steps), Franklin Christian Brown

Doctoral Dissertations

Sleep difficulties are becoming progressively more common among university students and may be related to increased academic and emotional difficulties. Psychoeducational interventions are among the most effective methods for reducing sleep difficulties and are significantly better than pharmacological treatments. Attempts to demonstrate the effectiveness of such treatments among university students are not present in the literature.

The purpose of this study was to develop the Sleep Treatment Education Program for Students (STEPS) and evaluate its effectiveness to improve sleep habits and reduce sleep complaints. The study used a double blind, experimental design. Participants in the treatment condition received Sleep Hygiene …

What's Happening: October, 2002, Maine Medical Center

What's Happening

No abstract provided.

Compounds Encoding The Protective M-Like Protein Of Streptococcus Equi And Assays Therefor, John F. Timoney, Sergey Artiushin

Veterinary Science Faculty Patents

The present invention relates generally to molecular compounds which encode the protective M-like protein of Streptococcus equi (SeM), the amino acid compound which is thereby encoded, and compositions of matter which incorporate either the encoding compounds or the cellular components for which they encode. For instance, vaccines which utilize the amino acid compounds or vectors and cell lines useful to make the amino acid compounds described herein are subjects of the present invention. The present invention provides methods to stimulate S. equi-specific immune response in horses. It also provides diagnostic assays for Streptococcus equi.

Electric Field Enhanced Plasmid Delivery To Liver Hepatocellular Carcinomas, Richard Gilbert, Mark J. Jaroszeski, Loree Heller, Richard Heller

Bioelectrics Publications

Electric field enhanced molecular delivery for cancer research and treatment is a new technology that has demonstrated its effectiveness in clinical trials using bleomycin or cisplatin (Heller, R., Gilbert, R., Jaroszeski, M. J. Clinical applications of electrochemotherapy, Advanced Drug Delivery Reviews 35,119-129, 1999), as chemotherapeutic agents. The technology is being investigated in research applications for applicability as a method to enhance gene expression in a target tumor. Success is predicated on an appropriate effective electric field mediated delivery protocol that triggers significant appropriate gene expression duration and levels. An electric field mediated delivery protocol includes a set of conditions …

Inside Unlv, Diane Russell, Gian Galassi, Cate Weeks

Inside UNLV

No abstract provided.

Unlv Magazine, Barbara Cloud, Bill Glovin, Andy Grossman, Mae Flennoy, Jennifer Vaughan

UNLV Magazine

No abstract provided.

Brain Uptake, Retention, And Efflux Of Aluminum And Manganese, Robert A. Yokel

Pharmaceutical Sciences Faculty Publications

My colleagues and I investigated the sites and mechanisms of aluminum (Al) and manganese (Mn) distribution through the blood-brain barrier (BBB). Microdialysis was used to sample non-protein-bound Al in the extracellular fluid (ECF) of blood (plasma) and brain. Brain ECF Al appearance after intravenous Al citrate injection was too rapid to attribute to diffusion or to transferrin-receptor-mediated endocytosis, suggesting another carrier-mediated process. The brain:blood ECF Al concentration ratio was 0.15 at constant blood and brain ECF Al concentrations, suggesting carrier-mediated brain Al efflux. Pharmacological manipulations suggested the efflux carrier might be a monocarboxylate transporter (MCT). However, the lack of Al …

Histological Evaluation Of Cryopreserved Ovaries In Autologous Abdominal Transplant To NaïVe, Outbred, Cd1, Mice, Wentia Elissa Ford

Biological Sciences Theses & Dissertations

This study showed that fresh and frozen-thawed mouse ovaries would maintain viable primordial or primary follicles post-autologous transplantation. Furthermore, with appropriate hormonal stimulation, these early-stage follicles were shown to develop into Graafian follicles containing a metaphase II antral ovum. Partially sliced ovaries were transplanted into the abdominal cavity of naive, outbred, CD1, white mice. Hormonal stimulation with pregnant mare serum gonadotropin (PMSG) and human chorionic gonadotropin (hCG) one week after the autologous transplantation stimulated variable degrees of follicle and ovum maturation within the ovarian tissue. There were no major morphological differences observed between the four groups, three treatment and one …

Electrically Mediated Delivery Of Vector Plasmid Dna Elicits An Antitumor Effect, L. Heller, D. Coppola

Bioelectrics Publications

In vivo electroporation is an efficient means of increasing plasmid DNA delivery to normal tissues, such as skin and muscle, as well as directly to tumors. In the experiments described here, plasmid DNA was delivered by in vivo electroporation to B16 mouse melanomas using two very different pulsing protocols. Reporter expression increased 21- or 42-fold, respectively with electroporation over injection alone. The growth of experimental melanomas with an approximate diameter of 4 mm on the day of treatment was monitored after electroporation delivery of reporter plasmid DNA. Remarkably, short-term complete regressions using one of these pulsing protocols occurred in up …

Mechanism Of Toxt-Dependent Transcriptional Activation At The Vibrio Cholerae Tcpa Promoter, Robin R. Hulbert, Ronald K. Taylor

Dartmouth Scholarship

The AraC homolog ToxT coordinately regulates virulence gene expression in Vibrio cholerae. ToxT is required for transcriptional activation of the genes encoding cholera toxin and the toxin coregulated pilus, among others. In this work we focused on the interaction of ToxT with the tcpA promoter and investigated the mechanism of ToxT-dependent transcriptional activation at tcpA. Deletion analysis showed that a region from −95 to +2 was sufficient for ToxT binding and activation, both of which were simultaneously lost when the deletion was extended to −63. A collection of point mutations generated by error-prone PCR revealed two small regions required …