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Structure Of Shiga Toxin Type 2 (Stx2) From Escherichia Coli O157:H7, Marie E. Fraser, Masao Fujinaga, Maia M. Cherney, Angela R. Melton-Celsa, Edda M. Twiddy, Alison D. O'Brien, Michael N. G. James

Uniformed Services University of the Health Sciences

Several serotypes of Escherichia coli produce protein toxins closely related to Shiga toxin (Stx) from Shigella dysenteriae serotype 1. These Stx-producing E. coli cause outbreaks of hemorrhagic colitis and hemolytic uremic syndrome in humans, with the latter being more likely if the E. coli produce Stx2 than if they only produce Stx1. To investigate the differences among the Stxs, which are all AB₅ toxins, the crystal structure of Stx2 from E. coli O157:H7 was determined at 1.8-Å resolution and compared with the known structure of Stx. Our major finding was that, in contrast to Stx, the active site of …

Intravenous 2-Deoxy-D-Glucose Injection Rapidly Elevates Levels Of The Phosphorylated Forms Of P44/42 Mitogen-Activated Protein Kinases (Extracellularly Regulated Kinases 1/2) In Rat Hypothalamic Parvicellular Paraventricular Neurons, Khan, Alan Watts

Departmental Papers (Biology)

No abstract provided.

What's Happening: January, 2004, Maine Medical Center

What's Happening

No abstract provided.

Renal Basolateral Transport Of Glucuronides And Other Organic Anions In Rat In Vitro Models, Sarah L. Miles

Theses, Dissertations and Capstones

Glucuronidation is a common Phase II biotransformation reaction that increases the hydrophilicity, and thus elimination, of toxins, xenobiotics, and endogenous compounds. Previous studies suggest that the kidney can secrete glucuronide conjugates, but the renal transport mechanisms for glucuronide secretion have not been determined. Based on the chemical nature of glucuronide metabolites, it is hypothesized that organic anion transporter (OAT) proteins along the basolateral membrane of the renal proximal tubule promote renal accumulation of glucuronide conjugates. The purpose of this study was to develop a rat renal proximal tubule model which demonstrates OAT activity and by which the contribution of OAT …

Translation Research: From Accurate Diagnosis To Appropriate Treatment, Craig P. Webb, Harvey I. Pass

Wayne State University Associated BioMed Central Scholarship

Abstract

This review article focuses on the various aspects of translational research, where research on human subjects can ultimately enhance the diagnosis and treatment of future patients. While we will use specific examples relating to the asbestos related cancer mesothelioma, it should be stressed that the general approach outlined throughout this review is readily applicable to other diseases with an underlying molecular basis. Through the integration of molecular-based technologies, systematic tissue procurement and medical informatics, we now have the ability to identify clinically applicable "genotype"-"phenotype" associations across cohorts of patients that can rapidly be translated into useful diagnostic and treatment …

Department Of Veterinary And Biomedical Sciences 2004 Annual Report

School of Veterinary and Biomedical Sciences: Information and History

Veterinary and Biomedical Sciences Personnel
VBMS Teaching Program
VBMS Research Program
International Activities, 2004
Veterinary Extension Program. 2004
VBMS Grants and Contracts Program. 2004
Refereed Publications by VBMS Faculty in 2004
Department of Veterinary and Biomedical Sciences. Selected Committees, Editorial and Other Appointments. 2004
Articles Regarding the Department in 2004
Departmental Budget Summaries. 2004
Nebraska Agricultural Statistics. 2003/2004

Wif-B Cells As A Model For Alcohol-Induced Hepatocyte Injury, Courtney S. Schaffert, Sandra L. Todero, Benita L. Mcvicker, Pamela L. Tuma, Michael F. Sorrell, Dean J. Tuma

United States Public Health Resources

A potential in vitro model for studying the mechanisms of alcohol-induced hepatocyte injury is the WIF-B cell line. It has many hepatocyte-like features, including a differentiated, polarized phenotype resulting in formation of bile canaliculi. The aim of this study was to examine the effects of ethanol treatment on this cell line. WIF-B cells were cultured up to 96 h in the absence or presence of 25 mM ethanol and subsequently were analyzed for ethanol-induced physiological and morphological changes. Initial studies revealed WIF-B cells exhibited alcohol dehydrogenase (ADH) activity, expressed cytochrome P4502E1 (CYP2E1), and efficiently metabolized ethanol in culture. This cell …

Natural And Technologic Hazardous Material Releases During And After Natural Disasters: A Review, Stacy Young, Lina Balluz, Josephine Malilay

United States Public Health Resources

Natural disasters may be powerful and prominent mechanisms of direct and indirect hazardous material (hazmat) releases. Hazardous materials that are released as the result of a technologic malfunction precipitated by a natural event are referred to as natural-technologic or na-tech events. Na-tech events pose unique environmental and human hazards. Disaster-associated hazardous material releases are of concern, given increases in population density and accelerating industrial development in areas subject to natural disasters. These trends increase the probability of catastrophic future disasters and the potential for mass human exposure to hazardous materials released during disasters. This systematic review summarizes direct and indirect …

Real-Time Fluorescence Pcr Assays For Detection And Characterization Of Heat-Labile I And Heat-Stable I Enterotoxin Genes From Enterotoxigenic Escherichia Coli, Udo Reischl, Mohammad T. Youssef, Hans Wolf, Eija Hyytia-Trees, Nancy A. Strockbine

United States Public Health Resources

To facilitate the diagnosis of enterotoxigenic Escherichia coli (ETEC) infections in humans, we developed and evaluated real-time fluorescence PCR assays for the Roche LightCycler (LC) against the enterotoxin genes commonly present in strains associated with human illness. Separate LC-PCR assays with identical cycling conditions were designed for the type I heat-labile enterotoxin (LT I) and the type I heat-stable enterotoxin (ST I) genes, using the LC hybridization probe format. A duplex assay for ST I with two sets of amplification primers and three hybridization probes was required to detect the major nucleotide sequence variants of ST I, ST Ia and …

West Nile Virus Blood Transfusion-Related Infection Despite Nucleic Acid Testing, Alexandre Macedo De Oliveira, Brady D. Beecham, Susan P. Montgomery, Robert S. Lanciotti, Jeffrey M. Linnen, Cristina Giachetti, Larry A. Pietrelli, Susan L. Stramer, Thomas J. Safranek

United States Public Health Resources

BACKGROUND: A case of West Nile virus (WNV) encephalitis associated with transfusion of blood that did not react when tested for WNV by minipool (MP) nucleic acid testing (NAT) is described. A Nebraska man developed clinical encephalitis 13 days after surgery and transfusion of 26 blood components. Antibody testing confirmed WNV infection. An investigation was initiated to determine the source of this infection.

STUDY DESIGN AND METHODS: The patient’s family members were interviewed to identify risk factors for WNV infection. Residual samples were retested for WNV RNA using transcription-mediated amplification (TMA) assay and two polymerase chain reaction (PCR) assays. …

Agricultural Pesticide Use And Adenocarcinomas Of The Stomach And Oesophagus, W. J. Lee, W. Lijinsky, E. F. Heineman, R. S. Markin, D. D. Weisenburger, M. H. Ward

United States Public Health Resources

Aims: To evaluate the risk of the stomach and oesophageal adenocarcinomas associated with farming and agricultural pesticide use.
Methods: Population based case-control study in eastern Nebraska. Telephone interviews were conducted with men and women diagnosed with adenocarcinoma of the stomach (n = 170) or oesophagus (n = 137) between 1988 and 1993, and controls (n = 502) randomly selected from the same geographical area. Unconditional logistic regression was used to calculate adjusted odds ratios (ORs) for farming and for use of individual and chemical classes of insecticides and herbicides, including pesticides classified as nitrosatable (able to form N-nitroso compounds on …

Molecular Characterization Of Noroviruses Detected In Diarrheic Stools Of Michigan And Wisconsin Dairy Calves: Circulation Of Two Distinct Subgroups, Annabel G. Wise, Stephan S. Monroe, Lora E. Hanson, Daniel L. Grooms, Donald Sockett, Roger K. Maes

United States Public Health Resources

Noroviruses have emerged as the leading worldwide cause of acute non-bacterial gastroenteritis in humans. The presence of noroviruses in diarrheic stool samples from calves on Michigan and Wisconsin dairy farms was investigated by RT-PCR. Norovirus-positive samples were found on all eight farms studied in Michigan and on 2 out of 14 farms in Wisconsin. Phylogenetic analyses of partial polymerase and capsid sequences, derived for a subset of these bovine noroviruses, showed that these strains formed a group which is genetically distinct from the human noroviruses, but more closely related to genogroup I than to genogroup II human noroviruses. Examination of …

Research Annual Report 2003, Children's Mercy Hospital

Research Annual Reports

Annual report of the research at Children's Mercy Hospital in Kansas City MO, a pediatric medical center.

Risk Of Disease From Wildlife Reservoirs: Badgers, Cattle, And Bovine Tuberculosis, M. Scantlebury, M. R. Hutchings, D. J. Allcroft, S. Harris

Michigan Bovine Tuberculosis Bibliography and Database

Livestock face complex foraging options associated with optimizing nutrient intake while being able to avoid areas posing risk of parasites or disease. Areas of tall nutrient-rich swards around fecal deposits may be attractive for grazing, but might incur fitness costs from parasites. We use the example of dairy cattle and the risks of tuberculosis transmission posed to them by pastures contaminated with badger excreta to examine this trade-off. A risk may be posed either by aerosolized inhalation through investigation or by ingestion via grazing contaminated swards. We quantified the levels of investigation and grazing of 150 dairy cows at badger …

Advancing Toward Universal Coverage: Are States Able To Take The Lead?, Lisa Dubay, Christina Moylan, Thomas R. Oliver

Journal of Health Care Law and Policy

No abstract provided.

Level Of Preparedness Of Patients For Medical Emergency, Waris Qidwai, Ss Ali, M Baqir, S Ayub

Department of Family Medicine

No abstract provided.

Meta-Analysis, Spatial, And Temporal Models To Analyze The Possible Association Between Childhood Leukemia In Proximity To Nuclear Facilities, Peter Baker

MUSC Theses and Dissertations

The purpose of this dissertation was to gain a better understanding of the possible association between childhood leukemia and nuclear facilities by conducting a meta-analysis and by the use of spatial and temporal models with respect to Pickering Nuclear Generator (PNG) in Ontario, Canada. The meta-analysis was conducted to combine and statistically analyze the many studies of childhood leukemia in the vicinity of nuclear facilities. Our focus was on studies that calculated SMRs or SIRs for individual nuclear sites. Due to variability between studies in defining age and geographic zones, eight separate analyses were performed based on age and zone …

Bioethical Malpractice: Risk And Responsibility In Human Research, Barbara A. Noah

Journal of Health Care Law and Policy

No abstract provided.

Aids And Adolescents, Rhonda Gay Hartman

Journal of Health Care Law and Policy

No abstract provided.

Mad Cow Disease: An Approach To Its Containment, Michael B. Abramson

Journal of Health Care Law and Policy

No abstract provided.

Commentary: Teratology In The 20th Century Environmental Causes Of Congenital Malformations In Humans And How They Were Established By Harold Kalter, Robert L. Brent

The Selected Works of Robert Brent

No abstract

Utilization Of Juvenile Animals Studies To Determine The Human Effects And Risks Of Environmental Toxicants During Postnatal Developmental Stages, Robert L. Brent

The Selected Works of Robert Brent

Toxicology studies utilizing animals and in-vitro cellular or tissue preparations have been used to study the toxic effects and mechanism of action of drugs and chemicals and to determine the effective and safe dose of drugs in humans and the risk of toxicity from chemical exposures. Testing in animals could be improved if animal dosing using the mg/kg basis was abandoned and drugs and chemicals were administered to compare the effects of pharmacokinetically and toxicokinetically equivalent serum levels in the animal model and human. Since alert physicians or epidemiology studies, not animal studies have discovered most human teratogens and toxicities …

Download Full Pdf Issue-Jefferson Translations-Vol. 1, No. 2, Spring 2004

Jefferson Translations

This issue includes the new Center for Translational Medicine, meet Dr. Walter Koch, medical oncology grows under Dr. Flomenberg, biodefense at Jefferson, Technology Transfer gives Jeff ideas broader access and the 125th gala honoring Dr. Bernard Segal.

Adherence To Antiretroviral Therapy In Adults: A Guide For Trainers, Horizons Program, International Centre For Reproductive Health, Mombasa Coast Province General Hospital

HIV and AIDS

Over the last five years, there has been a rapid change in treatment strategies for HIV infection. With the advent of newer antiretrovirals, treatment has moved from mono-therapy and bi-therapy to triple drug therapy or Highly Active Antiretroviral Therapy. One of the foremost concerns of ARV programs is the ability of people living with HIV/AIDS to maintain near perfect adherence over the long term. To achieve the goal of antiretroviral therapy (ART), undetectable levels of the virus in the blood, patients are required to maintain more than 90–95% adherence. Adherence is defined as a patient’s ability to follow a treatment …

Perceptions Of Pediatric Overweight And Weight Management: A Comparison Of Parents And Physicians, Lisa K. Thomason

Masters Theses & Specialist Projects

Pediatric overweight is of paramount importance in Kentucky, as studies show prevalence rates are higher in the state than the national average. Research suggests that comprehensive treatments involving diet training, exercise training, and psychological counseling are most effective at reducing overweight in children. Little research has been done, however, to compare parents' and physicians' perceptions of obesity, its treatment, or barriers to weight management.

The purpose of this study is to examine differences in attitudes toward pediatric obesity and weight management among parents and physicians, specifically related to the importance of diet training, exercise training, and psychological counseling. Surveys were …

Designing Educational Opportunities For The Hazards Manager Of The 21st Century: Workshop Report October 22-24, 2003, Deborah S. K. Thomas, Dennis S. Mileti

FMHI Publications

No abstract provided.

Access To Health, Natalie Huls

Human Rights & Human Welfare

Access to health is an often-overlooked aspect of the right to health. Without practical access, the right to health becomes an empty promise. International human rights conventions and declarations do not directly mention access to health, but the above comment on the International Covenant on Economic, Social, and Cultural Rights does address the issue.

Transitions To Adulthood In The Context Of Aids In South Africa: The Impact Of Exposure To Life Skills Education On Adolescent Knowledge, Skills, And Behavior, The Transitions To Adulthood Study Team

HIV and AIDS

This study is a collaborative research project of the University of Natal-Durban, the Horizons program, and Tulane University. One objective of the study was to contribute to understanding the effectiveness of life skills education in changing behavior in South Africa. The findings indicate substantial short-to-medium-term effects of exposure to life skills education on certain areas of sexual and reproductive health-related knowledge, confidence to use condoms, and condom use behavior. Further research is needed to assess whether the observed effects are transitory or long-term in nature, whether the observed behavioral changes have an impact on HIV infection rates among youth, and …

Improving The Reproductive Health Of Adolescents In Senegal, Nafissatou J. Diop, Heli Bathidja, Isseu Diop Toure, Thierno Dieng, Babacar Mane, Saumya Ramarao, Susan E. Adamchak, Emelita Wong, Adama Ndoye, Aboubacry Sy, Babacar Fall

Reproductive Health

In October 1999, the Frontiers in Reproductive Health (FRONTIERS) program began a three-year collaboration with the World Health Organization, the Senegal Ministries of Health, Education, and Youth, the Center for Research and Training in Health and Population, and the Population Training Group to test interventions to improve the reproductive health of youth aged 10–19. The community-based intervention included sensitization on adolescent reproductive health for community and religious leaders, reaching parents through women’s groups, and education sessions led by peer educators using a life-skills curriculum. As part of the clinic-based intervention, providers and peer educators were trained to offer youth-friendly services. …

Women Alcoholics And Addicts : An Examination Of The Role Of Gender-Specific Treatment Programs In Substance Abuse Counseling, Adarienne L. Burrow

Graduate Research Papers

Traditionally, substance abuse treatment programs have been developed based on data collected on male substance abusers. Women entering into these traditional treatment programs often are not successful at recovery. Male-based treatment programs are not designed to meet the complex set of needs of women substance abusers and addicts. The purpose of this research is two-fold. First, to identify the specific needs of women substance abusers and addicts. And secondly, determine how to address these identified needs in a substance abuse treatment program in order to provide a holistic approach to substance abusing and addicted women.