Digital Commons Network^™

Hypo-Responsiveness Of The Dental Pulp In Patients With Type 2 Diabetes And Hypertension: A Cross-Sectional Study, Kavitha M, Mallolu Anthony Sanjana Vijay, Niveditha B, Fathima A.I, Pavithra D, Vigneshwaran J

International Arab Journal of Dentistry

Introduction: Diabetes mellitus and hypertension, two of the most prevalent metabolic disorders worldwide, exert profound and progressive effects on multiple physiological systems. While their systemic complications are well documented, their subtle yet clinically significant impact on oral structures, particularly the dental pulp, remains underexplored.

Objectives: This study aims to assess the functional responsiveness of dental pulp nerves in individuals with type 2 diabetes mellitus and systemic hypertension, and to compare the findings with responses from healthy individuals, using an Electric pulp tester (EPT) as a diagnostic tool.

Methods: A total of 600 teeth from 75 adult patients (25 diabetic, 25 …

Minimally Invasive Decompression And Long-Term Follow-Up Of An Orthokeratinized Odontogenic Cyst Of The Mandible: A 7-Year Case Report, Akram Belmehdi, Karima El Harti

International Arab Journal of Dentistry

Introduction: Orthokeratinized odontogenic cyst (OOC) is a rare developmental jaw lesion with indolent behavior and low recurrence potential.

Case presentation: A 33-year-old healthy male was referred after incidental discovery of a mandibular radiolucency. Cone-beam CT revealed a well-defined unilocular lesion in close relation to the inferior alveolar canal. Aspiration confirmed its cystic nature, and a conservative decompression was performed using a rubber drain to reduce lesion size and preserve adjacent structures. Histopathologic examination identified orthokeratinized epithelium consistent with OOC. After seven months of decompression, the lesion showed marked ossification, allowing safe enucleation and peripheral curettage. Radiographic follow-ups at 19 months …

Comparative Evaluation Of A-Prf+ Or Collagen Membrane On Hard And Soft Tissue Changes Following Immediate Implant Placement: A Randomized Clinical Trial, Raagul S, Rahul Chopra, Sumit Malhotra, Prashansa Sharma, Ankur Tyagi, Nancy Singh

International Arab Journal of Dentistry

Objectives: To evaluate and compare clinical and radiographic parameters viz. Gingival Index (GI), Plaque Index (PI), Width of Keratinized Tissue (WKT), Midbuccal & Midlingual/Midpalatal soft tissue thickness (STT), ISQ values and crestal bone levels (CBL) using CBCT at baseline and 6 months in Group I (Immediate implant placement & A-PRF+) and Group II (Immediate implant placement & Collagen Membrane).

Methods: A total of 20 sites indicated for extraction were selected and randomly divided into: GROUP I : 10 sites were treated with immediate implant placement and covered with an A-PRF+ membrane and GROUP II : 10 sites were treated with …

Predictive Analysis For Success And Complications In Dental Implant Therapy Using Artificial Intelligence Models, Vishnu Priya Veeraraghavan, Aysha Jebin A, Isha Dusane, Govind Baheti, Ankita Agarwal, Santosh R Patil

International Arab Journal of Dentistry

Introduction: Dental implant therapy is a predictable treatment for tooth replacement, but its success is influenced by multiple patient, surgical, and systemic factors. Traditional statistical models, such as logistic regression, are limited in capturing nonlinear relationships among these variables. Artificial intelligence (AI) can integrate diverse predictors to enhance clinical risk assessment and improve treatment outcomes.

Objective: To identify key predictors of dental implant success and develop AI-based models capable of accurately forecasting implant outcomes using clinical and surgical parameters.

Methods: This retrospective cohort study analyzed data from 172 patients (219 implants) placed between 2020 and 2021. Patient demographics, systemic health …

Specific Carbohydrate Diet Versus Mediterranean Diet In Adult Patients With Mild To Moderate Ulcerative Colitis: A Randomized Controlled-Feeding Trial, Albert Sheng-Yin Chen, Long Nguyen, Brianna Gray, Katherine Williams, Jenny Gurung, Lauren Canha, Jessica Mcgoldrick, Jane Hubbard, Hamed Khalili

Student Papers, Posters & Projects

Background and aims: This pilot randomized controlled-feeding trial compared the effect of Specific Carbohydrate Diet (SCD) and Mediterranean diet (MeD) in mild to moderate ulcerative colitis (UC).

Methods: Seventeen adults were randomized to a 6-week SCD (n = 8) or MeD (n = 9) intervention. Primary outcome was change in partial Mayo Clinic score (pMCS).

Results: The study was discontinued early due to significant dropout (n = 9, 52.9%). There was no significant between-group differences observed for pMCS change (SCD, -0.8; MeD, -1.3; p = 0.499) or secondary outcomes. Exploratory metagenomic analysis revealed enrichment of Parasutterella excrementihominis …

Progesterone And Cerclage Together For Short Cervix: Evaluating Potential Complementary Effects For Improved Preterm Birth Prevention, Vincenzo Berghella, Ilaria Paladino, Moti Gulersen, Lauren Sayres, Amanda Roman, Rupsa C. Boelig

Department of Obstetrics and Gynecology Faculty Papers

Management of patients with a short cervical length (CL) on transvaginal ultrasound (TVU) often involves therapy with vaginal progesterone and/or cervical cerclage. Although these interventions are frequently viewed as alternative strategies, they may also represent complementary approaches targeting different mechanisms involved in spontaneous preterm birth. As in other areas of medicine, therapies with different mechanisms are often used together rather than as mutually exclusive options. In line with current level 1 evidence from randomized controlled trials (RCTs) and meta-analyses of RCTs on either vaginal progesterone or cerclage, in singleton gestations with no prior spontaneous preterm birth (SPTB), vaginal progesterone is …

Spontaneous Intracranial Hypotension With Negative Brain Mri Findings: A Systematic Review Of Diagnostic Strategies And Clinical Outcomes, Marina Romozzi, Giuseppe Garignano, Valid Rastegar, Francesco Onorati, Catello Vollono, Federico Tosto, Masahito Katsuki, Yasuhiko Matsumori, Francesco Signorelli

Student Papers, Posters & Projects

BACKGROUND: Spontaneous intracranial hypotension (SIH) is a debilitating syndrome typically characterized by orthostatic headache and classic brain MRI findings. However, brain MRI abnormalities are indirect manifestations of cerebrospinal fluid (CSF) volume depletion rather than direct evidence of the leak, and some patients may present with unrevealing or absent typical brain MRI findings, posing a considerable diagnostic challenge.

METHODS: A comprehensive search of PubMed/Medline, Web of Science, and Scopus was conducted to perform a systematic review, according to PRISMA guidelines, analyzing the available evidence on MRI-negative SIH (patients without typical SIH findings on their initial brain MRI), including its clinical features, …

Decoding The Allosteric Grammar Of Protein Kinases: A Dual-Stream Framework Integrating Protein Language Models And Energy Landscape Frustration Analysis, Will Gatlin, Max Ludwick, Lucas Turano, Brandon Foley, Kamila Riedlova, Vít Škrhák, Marian Novotný, David Hoksza, Gennady M. Verkhivker

Mathematics, Physics, and Computer Science Faculty Articles and Research

The spatial and energetic encoding of allosteric regulatory sites remains a major challenge in structural biology, frequently representing a “blind spot” for sequence-based artificial intelligence (AI) models. We present a protein language model (PLM)-guided approach complemented by the energy landscape frustration analysis as a dual-stream framework to investigate the relationship between AI prediction of binding sites and biophysical organization of regulatory pockets across the human kinome. By probing a fine-tuned residue-level PLM classifier across 453 kinase structures, a clear performance gap is discovered between highly predictable orthosteric pockets (Types I, I.5, and II) and poorly resolved distal allosteric sites (Type …

Antropometri Tangan Penduduk Kota Ambon Sebagai Dasar Perancangan Ergonomis, Puan Maryam Leaongso, Wilma Latuny, Aminah Soleman

National Journal of Occupational Health and Safety

The limited availability of hand anthropometric data for the Indonesian population, particularly for the residents of Ambon City, may affect the accuracy of designing ergonomic products, work equipment, and public facilities. This study aimed to measure and analyze 25 hand dimensions of the Ambon City population based on gender and age, and to compare the measurement results with national anthropometric data. This research employed a quantitative method with a descriptive design through an anthropometric approach. Data were collected directly from 200 respondents, consisting of 100 females (aged 18–40 years) and 100 males (aged 20–40 years). Data analysis was performed using …

Recent Advances In Alzheimer's Disease: From Molecular Mechanisms To Therapeutic Strategies, Michelle D Rudman, Jason D Ulrich, David M Holtzman

2020-Current year OA Pubs

Alzheimer's disease (AD) remains the leading cause of dementia worldwide and an escalating global health crisis. The hallmark amyloid plaques and neurofibrillary tangles (NFT) are now known to be accompanied by a complex array of pathologies that culminate in neurodegeneration and cognitive decline. New disease-modifying therapies for AD can now slow cognitive decline through the removal of amyloid plaques from the brain, but treatments to stop or prevent cognitive impairment remain elusive. In this review, we summarize the most recent updates in AD research on pathologic disease mechanisms and therapeutic strategies, highlighting advancements in apolipoprotein E (APOE) biology, neuroimmunology, biomarker …

Molecular Detection Of Chlamydiales In Captive Zoo Animals At Songkhla Zoo, Southern Thailand, Rawikan Inchuai, Sunisa Khongthong, Boontarikaan Wititkornkul, Naruepan Wongpluksin

The Thai Journal of Veterinary Medicine

Several species of Chlamydia are recognized as significant zoonotic pathogens that infect a wide range of hosts, including humans. This study aimed to investigate the occurrence and genetic characteristics of Chlamydiales–related bacteria in captive animals at Songkhla Zoo, southern Thailand. A total of 92 cloacal and rectal swab samples were collected from mammals (n = 22), birds (n = 55), and reptiles (n = 15). Genomic DNA was screened using polymerase chain reaction (PCR) targeting the Chlamydiales 16S rRNA gene and the ompA gene specific for Chlamydia psittaci. Positive amplicons were subjected to Sanger sequencing and phylogenetic analysis. A …

Unbiased Avidity-Based Isolation Of Antigen-Specific T Cells, Amanda Montoya, Meredith L Frank, Peixin Jiang, Hui Nie, Minying Zhang, Emily Bontekoe, Jared K Slone, Ludovica L Posta, Sofia Rosy Caterina Sorice, Tina Cascone, Maura Gillison, Don L Gibbons, Jianjun Zhang, Eleonora Dondossola, Lydia Kavraki, Pamela L Wenzel, John V Heymach, Alexandre Reuben

The Brown Foundation: Institute of Molecular Medicine

Background: Cancer immunotherapies have significantly improved treatment efficacy and patient survival by exploiting antigen-specific T cells to eliminate cancer cells. However, current approaches for identifying and isolating antigen-specific T cells typically require prior knowledge of target antigens, limiting discovery, and reducing the ability to consistently detect rare tumor-reactive T cells. We therefore sought to develop an unbiased platform for the identification and enrichment of antigen-specific T cells using naturally processed and presented tumor antigens.

Methods: We developed ATTACH (Assessment of T cells Tethered to Antigen Class I Histocompatibility), a microfluidic platform that applies controlled shear stress and leverages tumor cells …

Identification Of Active Compounds And Antidiabetic Activity Of Red Shoot (Syzygium Myrtifolium) Leaves Extract With In Vitro And In Silico Methods, Muhamad Iqbal Prakoso, Siti Warnasih

Pharmaceutical Sciences and Research

Diabetes mellitus (DM) is a chronic metabolic disease that affects approximately 537 million people worldwide (1 in 10 individuals), and its prevalence is projected to reach 783 million by 2045. One of the plants that has the potential to be an antidiabetic is red shoots (Syzygium myrtifolium) since it contains various secondary metabolite compounds such as flavonoids, triterpenoids, steroids, saponins, phenolics, and anthocyanins. This study aimed to evaluate the antidiabetic properties of red shoot leaves extract and to identify and predict interactions between its active compounds and the α-glucosidase receptor. Ethanol, ethyl acetate, and n-hexane extracts of red …

Online Decoding Of Rat Self-Paced Locomotion Speed From Eeg Using Recurrent Neural Networks, Alejandro De Miguel, Nelson Totah, Uri Maoz

Psychology Faculty Articles and Research

Objective. Accurate and reliable neural decoding of locomotion holds promise for advancing clinical applications such as rehabilitation and prosthetic control, as well as for understanding neural correlates of action. Recent studies have demonstrated successful decoding of locomotion kinematics across species in motorized treadmill settings. However, efforts to decode locomotion speed directly and continuously in more natural contexts—where pace is self-selected rather than externally imposed—are scarce, and those that exist generally achieve only modest accuracy and require intracranial implants. Here, we aim to decode self-paced locomotion speed non-invasively and continuously using cortex-wide electroencephalography (EEG) recordings from rats. Approach. We introduce an …

Evaluating Cardiotoxicity And Factors Associated With In-Hospital Mortality Among Emergency Department Patients With Substance Use Disorder: An 11-Year Retrospective Study, Darpanarayan Hazra, Nadia Ahmed Said Alqusaimi, Alyaqeen Ahmed Abdullah Al Breiki, Raghad Saleh Mohammed Al Akhzami, Aya Hasan Hadi Aalhamad, Suad Abdullah Al Abri

Sultan Qaboos University Medical Journal

Objective: Substance use disorder (SUD)-related emergencies are increasing globally, with cardiotoxicity contributing substantially to morbidity and mortality. This study evaluated cardiotoxicity patterns and factors associated with in-hospital mortality among adults with SUD presenting to the emergency department (ED).Methods: This retrospective observational study was conducted from January 2014 to December 2024 at Sultan Qaboos University Hospital, University Medical City, Muscat, Oman. Adults aged ≥18 years with SUD and positive urine drug screening (UDS) were included if they had elevated troponin (>14 ng/L), electrocardiographic abnormalities or echocardiographic structural dysfunction. Clinical, laboratory, imaging, management and outcome data were analysed. Results …

Tgf-Β Drives The Conversion Of Conventional Nk Cells Into Uterine Tissue-Resident Nk Cells To Support Murine Pregnancy, Josselyn D Barahona, Liping Yang, D Michael Nelson, Wayne M Yokoyama

2020-Current year OA Pubs

Tissue microenvironments shape lymphocyte differentiation to align immune function with local physiological demands. Uterine natural killer (NK) cells are critical for reproductive success, yet the molecular cues in the uterus that instruct their specialized identities remain incompletely understood. Here, we identify a TGF-β-dependent differentiation pathway by which circulating conventional NK cells convert into uterine tissue-resident NK cells during murine pregnancy. Loss of TGF-β receptor II expression in

Perceived Sensitivity Of Sensor-Based Digital Health Data: Qualitative Interview Study, Christine Deeney, Anika Sonig, Meghan E Hurley, Birkan Tunç, Eric A Storch, John D Herrington, Jennifer Blumenthal-Barby, Kristin Kostick-Quenet

Center for Medical Ethics and Health Policy Staff Publications

Background: Digital health tools are increasingly used in mental health care to passively collect patient data and analyze health status outside of clinical settings. While technologies such as digital phenotyping, affective computing, and computational behavioral analysis offer new insights into symptom manifestation in daily life, they generate large volumes of potentially sensitive data that raise significant data privacy concerns, requiring high levels of patient awareness and consent. Empirical research is lacking on stakeholder understandings toward the sensitivity of these data and expectations for data stewardship, perspectives that are critical for developing robust informed consent and data protection policies for digital …

Noda News Summer 2026

New Orleans Dental Association News

New Orleans Dental Association (NODA) News includes regular columns with regional news impacting the New Orleans membership including: columns from the President and Executive Director, feature articles relating to the field of dentistry, association events and upcoming activities, component news, continuing education opportunities, and classifieds.

Efficacy Of Sirolimus Versus Paclitaxel-Coated Balloons For Coronary Revascularization: An Updated Meta-Analysis With Grade Assessment, Matthew Aldo Wijayanto, Christopher Daniel Tristan, Erlangga Masykur Kynaya, Annisa Aghnia Rahma, Putri Saskia Harimurti, Arwin Saleh Mangkuanom, Amir Aziz Alkatiri, Doni Firman, Bambang Budi Siswanto

Journal of the Saudi Heart Association

Drug-coated balloons are an emerging strategy for coronary revascularization. Despite their increasing use, the comparative efficacy of sirolimus- versus paclitaxel-coated balloons (SCB vs PCB) remains uncertain. This updated meta-analysis evaluated clinical and angiographic outcomes of SCB and PCB in percutaneous coronary intervention (PCI). A comprehensive search of PubMed, Scopus, and ScienceDirect was performed on August 28, 2025. Studies directly comparing SCB and PCB were included. The primary endpoint was target lesion failure (TLF), which was defined as cardiac death, target vessel myocardial infarction (TVMI), or target lesion revascularization (TLR). On follow-up angiography, secondary endpoints consisted of minimal lumen diameter (MLD), …

Evaluation Of Diclazuril And Amprolium Against Eimeria Spp. In Vietnamese Broilers: Implications For Intestinal Integrity And Claudin Gene Expression, Nguyen Thi Hoa, Pham Hoang Son Hung, Le Dinh Phung, Le Duc Thao, Nguyen Van Chao, Tran Thi Na, Tran Cao My Linh, Ho Thi Dung

The Thai Journal of Veterinary Medicine

This study aimed to assess the resistance status of Eimeria spp. in Vietnam to diclazuril and amprolium and to evaluate their effects on intestinal recovery following infection. One hundred 12-day-old chicks were randomly allotted to four groups: a non-infected negative control (NC), an infected untreated as positive control (PC), an infected group treated with diclazuril (DI), and an infected group treated with amprolium (AM). At day 14, chicks from all groups except NC were orally challenged with 2 × 10⁴ sporulated Eimeria oocysts (E. tenella, E. maxima, E. acervulina, and E. mitis). On day 7 …

Phenomenological Analysis Of Trust In Science For The Design Of Ai-Based Health And Social Policies, Kochav Koren

All Duquesne Faculty Scholarship

The promises of artificial intelligence (AI) as a solution to challenges facing health systems increasingly link technological innovation with improvements in efficiency and access. However, the legitimacy of AI-supported health policies depends not only on scientific competence but also on public trust in scientific institutions. Using data from the international Trust in Science Project (71,922 respondents across 68 countries), this study analyses multidimensional trust and communication ecologies to reconstruct relational configurations of trust, termed ‘Figures of Relevance’. Findings reveal that whilst scientific competence is widely recognised, perceptions of institutional openness vary markedly, shaping heterogeneity in trust. The study identifies four …

Effect Of Virtual Reality Intervention Of Postoperative Pain In Pediatric Surgical Patients, Tara L. Kempker

Dissertations

Problem: Pediatric surgical procedures are associated with preoperative anxiety. Preoperative anxiety is associated with higher postoperative pain. Multiple studies have found that decreasing preoperative anxiety using Virtual Reality (VR) Intervention also decreases postoperative pain scores and use of rescue analgesics in postoperative care units (PACU). However, there is no standardized protocol for use of VR interventions and in our institution, VR was not utilized at all.

Methods: This quality improvement (QI) initiative utilized a quasi-experimental design and convenience sampling. Quantitative data was collected during visits for scheduled operative visits for pediatric surgical patients ages seven to 18 years …

Identification Of Prognostic Factors For Disease Recurrence-Free Survival (Drfs) In Breast Cancer Patients Using The Cox Proportional Hazards Model, Dwi Cahyaning Putri, Veny Prabaningrum, Samsul Arifin

Jurnal Biostatistik, Kependudukan, dan Informatika Kesehatan

Breast cancer remains one of the most common malignancies among women worldwide, with recurrence remaining a major clinical challenge. Despite various treatment efforts, the mortality rate from breast cancer remains high, particularly among patients with worse clinical and molecular conditions. This study aims to identify factors influencing the survival of breast cancer patients using the Cox proportional hazards model. The study analyzes clinical and molecular data of breast cancer patients to identify key risk factors affecting Disease Recurrence-Free Survival (DRFS). Analysis was conducted using Kaplan-Meier, the Log Rank test, and the Cox proportional hazards model. The results showed that nodal …

Fecal Fatty Acids And Other Risk Factors Associated With Multiple Sclerosis Score, Progression And Diagnosis Among Hispanic Adults, Juliana Ayala-Viqueira

Walden Dissertations and Doctoral Studies

Multiple sclerosis (MS) is a chronic neurological condition with insufficient research regarding risk factors influencing MS severity score (MSSS), progression and diagnosis among Hispanic adults living in the United States. This cross-sectional study assessed factors associated with MS score, progression and diagnosis using secondary data from the International Multiple Sclerosis Microbiome Study (iMSMS). Guided by the ecological model, the study included N = 576 participants with MS. Manuscript 1 explored the association between body mass index (BMI), sodium consumption, and the MS severity score in a Hispanic adult population. A multiple linear regression was conducted to examine whether age and …

Pneumococcal Colonization In Pediatric Patients Undergoing Hematopoietic Cell Transplantation, Liset Olarte, Jennifer E Schuster, Ibrahim Ahmed, Kristina G Hulten

Faculty, Staff and Students Publications

Despite the increased risk of invasive pneumococcal disease among pediatric hematopoietic cell transplant (HCT) recipients, pneumococcal colonization has not been evaluated in this population. We prospectively assessed longitudinal pneumococcal colonization in 22 pediatric HCT recipients from conditioning therapy to 100 days post-HCT. A higher likelihood of pneumococcal colonization was observed in the late post-HCT period (weeks 9-14) compared with the early post-HCT period (weeks 1-8), with odds increasing with each additional week after conditioning.IMPORTANCEThis is the first study to longitudinally evaluate pneumococcal colonization in pediatric hematopoietic cell transplant (HCT) recipients from the onset of conditioning therapy to 100 days post-HCT. …

Tau Pathology In Epilepsy: Emerging Mechanisms And Translational Opportunities, Arjune Sen, Xin You Tai, Aristea Galanopoulou, Maria Thom, Eleonora Aronica, Lucy Vivash, Martin Hardmeier, Action Amos, Stephan Rueegg, Matthias Koepp, Yaroslav Winter, Christoph Helmstaedter, Jeffrey L Noebels, Hilal A Lashuel, Terence J O'Brien

Faculty, Staff and Students Publications

The onset of epilepsy in adulthood occurs most commonly after 55 years of age. Given the ageing global population, this disorder represents an increasing burden on healthcare and society. The bidirectional link between epilepsy and dementia is a focus of intense research with underlying tau pathology highlighted as a potential mechanistic link. In this review, we examine the evidence for tau-related neurodegenerative processes in epilepsy beginning with how changes in biochemical and structural properties of the tau protein can lead to abnormal phosphorylation and pathological aggregation. We consider the role of tau in seizure occurrence and cognitive difficulties in experimental …

Cytoskeletal Protein Expression In Autism Spectrum Disorder: A Psychoneuroimmunological Perspective On Sars-Cov-2, James Birch

Walden Dissertations and Doctoral Studies

The COVID-19 pandemic heightened concerns about health disparities among individuals with autism spectrum disorder (ASD). Limited understanding of the biological factors underlying health variability may hinder efforts to reduce disparities and improve care for neurodivergent populations. Guided by the psychoneuroimmunology framework, this quantitative study investigated whether cytoskeletal gene expression was associated with neuroimmune variability in ASD and SARS-CoV-2 populations. Three genes –Nck-associated protein 1 (NCKAP1), WAS/WASL-interacting protein family member 1 (WIPF1), and actin-related protein 2/3 complex subunit 1B (ARPC1B)—were evaluated using two publicly available Gene Expression Omnibus datasets GSE212645 (ASD vs. sibling-matched controls, N = 40) and GSE152641 (SARS-CoV-2-positive vs. …

Fostering Active Learning In Reproductive Health Classes Among Undergraduates Of A Nigerian Public University: The Role Of Cognitive Restructuring Health Education, Ndubuisi Friday Ugwu, Adewumi Segun Igbinlade, Adijat Bolanle Adams, Uchenna Anselm Anibueze, Gabriel Aderibigbe Oyegbami, Chinyere Chrysantus Onwurah, Sabina Nwakaego Igwilo, Olufemi M. Olubamise, Folasade Adediran, Oladipo Adeyeye Olubodun Phd, Jacob Kehinde Opele, Loveth Nnenna Ugwu, Amos Olorunwa Agbojo

Higher Learning Research Communications

Objective: This study examined the efficacy of the cognitive restructuring health education (CRHE) intervention in improving participatory learning in sexual and reproductive health classes among students at a Nigerian public university.

Methods: The study employed a randomized pretest/posttest control group design involving 130 undergraduates from Federal University Oye-Ekiti, Nigeria. Participants were randomly assigned to either a 2-week intensive health education–based cognitive restructuring program (n = 65) or a waitlist control group (n = 65). Interaction behavior was assessed at pretreatment, at post-treatment, and at a 6-week follow-up using the 14-item Interaction Behavior Checklist (reliability = 0.85). …

Longitudinal Genomic Surveillance Of Sars-Cov-2 In A University Microcosm Reflects Global Evolutionary Trends, Sankar Prasad Chaki, Melissa M Kahl-Mcdonagh, Benjamin W Neuman, Loni A Taylor, Rebecca S B Fischer, Blake M Hanson, Marlisa S Hardy, Jo Ann Culpepper, Eric Boerwinkle, Kurt A Zuelke

Faculty, Staff and Student Publications

SARS-CoV-2 transmission has declined, yet ongoing viral evolution continues to pose public health challenges. This study conducted long-term (2020-2024) genomic surveillance within a large and globally diverse university community. Viral genomes obtained from clinical (n = 1,786) and surveillance (n = 888) testing samples were classified under clade, lineage, and variant systems, and compared with global genomic data sets. Local patterns broadly paralleled global evolutionary trends, including the phased emergence of major variants, episodic introductions of new lineages, and shifts in dominant clades. Lineage diversity peaked during fall semesters and was highest among undergraduate-aged individuals, with similar distributions …

Biophysical Insights Into A Cryptic Ligand Site In The Hydrophobic Core Of Human Pcna, Alexis N. Dispensa, Sharon N, Greenwood, Jason Yang, Daniel E. Logatto, James Fusco, E. Railey White, Roderic G. Eckenhoff, Zhiwei Liu, Brian P. Weiser

Department of Anesthesiology Faculty Papers

Proteins contain small pockets that form due to imperfections in residue packing or the rotational and conformational movement of amino acids. In this work, we used the fluorescent probe 1-aminoanthracene (AMA) to detect a small ligand pocket in the hydrophobic core of human proliferating cell nuclear antigen (PCNA), which is a critical protein for DNA replication and repair. Fluorescence measurements of AMA reported that the core of PCNA had a dielectric constant (ε) of 4, which was very apolar and similar to cyclohexane (ε = 2). Protein mutagenesis, photoaffinity labeling, and molecular dynamics simulations localized the binding site for AMA …