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Phenylbutyl Nitrone Compositions And Methods For Prevention Of Gastric Ulceration, John M. Carney, Robert A. Floyd

Pharmacology and Nutritional Sciences Faculty Patents

Compositions containing PBN, or active derivatives thereof, in a suitable pharmaceutical carrier for administration to a patient, are disclosed for treating or preventing gastric ulceration caused by ingestion of non-steroidal anti-inflammatories. Based on animal studies, the dosage is in the range of 3 to 300 mg/kg and is administered prior to, simultaneously, or shortly after ingestion of the NSAID compounds(s). In the preferred embodiment, the range is between 10 and 30 mg/kg, depending on the dosage unit required to protect the mucosa. The preferred method of administration is orally, alone or in combination with the non-steroidal anti-inflammatory. It is believed …

2,4-Disulfonyl Phenyl Butyl Nitrone, Its Salt And Their Use As Pharmaceuticals, John M. Carney

Pharmacology and Nutritional Sciences Faculty Patents

2,4-disulfonyl α-phenyl-tert-butyl nitrone and its pharmaceutically acceptable salts are disclosed. These materials are useful as pharmaceutical agents for oral or parenteral, e.g. intravenous administration to patients suffering from acute central nervous system oxidation as occurs in a stroke or from gradual central nervous system oxidation which can exhibit itself as progressive central nervous system function loss. The materials are also used to ameliorate the side effects of oxidative-damage causing antineoplastic disease treatments.

The Discriminative Stimulus Properties Of Morphine And U-50,488h In A Three-Key Assay: A Mu And Kappa Opioid Discrimination In The Pigeon, Malath Makhay

Dissertations

Opiate drugs have been classified in two-choice assays according to their ability to produce generalization in animals to the prototypicix opiate, morphine, versus vehicle, or to the k opioid, U -50,488H versus vehicle injections (Picker & Dykstra, 1987). A three-choice discrimination procedure, in which subjects discriminate among morphine, U -50,488H , and vehicle injections, might afford a greater degree of precision in characterizing the subjective effects of opioids. The feasibility of such a procedure was demonstrated in the present study, in which five pigeons were trained to discriminate among injections of 5 .6 m g/kg morphinel 5 .6 m g/kg …

A Tandem Duplication Within The Fibrillin 1 Gene Is Associated With The Mouse Tight Skin Mutation., Linda D. Siracusa, Rodney Mcgrath, Qing Ma, John J. Moskow, Jayanthi Manne, Paul J. Christner, Arthur M. Buchberg, Sergio A. Jimenez

Department of Medicine Faculty Papers

Mice carrying the Tight skin (Tsk) mutation have thickened skin and visceral fibrosis resulting from an accumulation of extracellular matrix molecules. These and other connective tissue abnormalities have made Tskl + mice models for scleroderma, hereditary emphysema, and myocardial hypertrophy. Previously we localized Tsk to mouse chromosome 2 in a region syntenic with human chromosome 15. The microfibrillar glycoprotein gene, fibrillin 1 (FBN1), on human chromosome 15q, provided a candidate for the Tsk mutation. We now demonstrate that the Tsk chromosome harbors a 30- to 40-kb genomic duplication within the Fbn1 gene that results in a larger than normal in-frame …

Toxoplasma Gondii-Induced Immune Suppression By Human Peripheral Blood Monocytes: Role Of Gamma Interferon., Jacqueline Y. Channon, Lloyd H. Kasper

Dartmouth Scholarship

The ability of Toxoplasma gondii to evade the host immune response during primary infection in humans is poorly understood. In murine toxoplasmosis, infected spleen macrophages release soluble factors that mediate a transient immunosuppression, which may allow the parasite to become established. When an enriched population of human monocytes from seronegative individuals was incubated with toxoplasmas in vitro, soluble factors that mediated market suppression of mitogen-induced lymphocyte DNA synthesis were released. Irradiated tachyzoites that do not undergo replication were sufficient stimuli for near-maximal soluble factor release. Up to 50% of the soluble factor-mediated suppression is attributable to a gamma interferon (IFN-gamma)-dependent …

Disruption Of The Cbfa2 Gene Causes Necrosis And Hemorrhaging In The Central Nervous System And Blocks Definitive Hematopoiesis., Qing Wang, Terryl Stacy, Michael M Binder, Miguel Marin-Padilla, Arlene H. Sharpe, Nancy A. Speck

Dartmouth Scholarship

The CBFA2 (AML1) gene encodes a DNA-binding subunit of the heterodimeric core-binding factor. The CBFA2 gene is disrupted by the (8;21), (3;21), and (12;21) chromosomal translocations associated with leukemias and myelodysplasias in humans. Mice lacking a CBF alpha 2 protein capable of binding DNA die between embryonic days 11.5 and 12.5 due to hemorrhaging in the central nervous system (CNS), at the nerve/CNS interfaces of cranial and spinal nerves, and in somitic/intersomitic regions along the presumptive spinal cord. Hemorrhaging is preceded by symmetric, bilateral necrosis in these regions. Definitive erythropoiesis and myelopoiesis do not occur in Cbfa2-deficient embryos, and disruption …

Correlates Of The Mmpi-A Immaturity (Imm) Scale In An Adolescent Psychiatric Population, Eric Albert Imhof

Psychology Theses & Dissertations

Despite numerous theories of adolescent ego development, there is no objective measure of ego development or maturation targeted for the assessment of adolescents. The Immaturity (IMM) Scale developed for the Minnesota Multiphasic Personality Inventory - Adolescent (MMPI-A) was designed to fill this void in adolescent assessment. The present study examined the concurrent validity of the IMM Scale based on sixty-six adolescents (13-18 years old) in an inpatient setting using four prominent measures of maturity, i.e., The Defining Issues Test (DIT), the Washington University Sentence Completion Test (WUSCT), the Extended Objective Measure of Ego Identity Status - Second Revision (EOM-EIS-2), and …

Relationship Between Design Marketing Strategies And Toothbrush Purchases By Consumers, Cynthia Lynn Garvin

Dental Hygiene Theses & Dissertations

The purpose of this study was to investigate whether a relationship exists between design marketing strategies of toothbrushes (handle size, color, and shape; brush head size and shape; and bristle firmness and formation of toothbrushes) and toothbrush purchases made by consumers. To understand what consumers preferred, a self-designed, 24 item questionnaire, "Toothbrush Design and Consumer Preferences," was given to a convenience sample of 300 subjects ranging from ages 20 to 60 and over from the Old Dominion University campus in Norfolk, Virginia. Responses from each item were analyzed using frequency and percentages of responses, contingency tables, Kendall's tau b, and …

The Assessment Of Osteoinductivity Of Human Allograft Demineralized Bone Matrix By In Vivo And In Vitro Assay Models, Min Zhang

Theses and Dissertations in Biomedical Sciences

The osteoinductive potential (osteoinductivity) is a very important aspect of demineralized bone matrix (DBM) as a biomaterial. This study is directed at establishing qualitative and quantitative assays of the osteoinductivity of human DBM. Further aspects of this study include an assessment of the effects of the production process, matrix characterization, and donor related factors on osteoinductivity of the DBM.

Athymic mice were chosen as the animal model for use in the in vivo bioassay. Implantation of DBM in muscle pouches provided for much higher osteoinductivity (remineralization to 10% calcium) than subcutaneous site implantation (remineralization to 1.6% calcium). Histological observations revealed …

An Enzyme-Linked Immunosorbent Assay For The Detection Of Cardiac Myoglobin Using Monoclonal Antibodies, Deborah F. Kelly

Chemistry & Biochemistry Theses & Dissertations

The most prevalent life-threatening disease worldwide, secondary to coronary artery thrombosis is Acute Myocardial Infarction (AMI). Increases in three clinical markers have been explored as an early means of AMI detection: Cardiac myoglobin, and the two specific cardiac isoforms of Creatine Kinase, CK-MM and CK-MB. However, myoglobin levels have shown to give a more accurate correlation (R = 0.89) than does Creatine Kinase evaluation. Therefore, we have developed a two-site Enzyme-Linked Immunosorbent Assay (ELISA) using monoclonal antibodies against myoglobin isolated from human heart tissue. This assay is based on specific anti-genantibody interactions and is sensitive at the nanogram level. We …

Conditioned Stimulus Determinants Of Conditioned Response Form In Pavlovian Fear Conditioning, Stephen D. Kim, Steven Rivers, Rick A. Bevins, John J. B. Ayres

Department of Psychology: Faculty Publications

Four experiments using barpress conditioned suppression in rats found that tone evoked more freezing (immobility) than did light. Still, tone and light appeared to have similar conditioned value as assessed by suppression in Experiments 1, 2, and 3, and by blocking, second-order conditioning, and over-conditioning assays in Experiments 1, 2, and 3, respectively. Experiment 4 arranged for tone to evoke less suppression than light but more freezing. Results suggest that in fear conditioning, the nature of the conditioned stimulus affects the form of conditioned responding (strong vs. weak freezing). This conclusion extends one drawn by P. C. Holland (1977) on …

Identification Of A Brazil-Nut Allergen In Transgenic Soybeans, Julie A. Nordlee, Steve L. Taylor, Jeffrey A. Townsend, Laurie A. Thomas, Robert K. Bush

Department of Food Science and Technology: Faculty Publications

Background: The nutritional quality of soybeans (Glycine max) is compromised by a relative deficiency of methionine in the protein fraction of the seeds. To improve the nutritional quality, methionine-rich 2S albumin from the Brazil nut (Bertholletia excelsa) has been introduced into transgenic soybeans. Since the Brazil nut is a known allergenic food, we assessed the allergenicity of the 2S albumin.

Methods: The ability of proteins in transgenic and nontransgenic soybeans, Brazil nuts, and purified 2S albumin to bind to IgE in serum from subjects allergic to Brazil nuts was determined by radioallergosorbent tests (four …

Biochemical And Mutational Analysis Of The Histidine Residues Of Staphylococcal Enterotoxin A., M Hoffman, M Tremaine, J Mansfield, M Betley

Manuscripts, Articles, Book Chapters and Other Papers

The goal of this study was to examine the role of histidine residues in the biological activities of staphylococcal enterotoxin A (SEA). Carboxymethylated SEA was unable to stimulate murine T-cell proliferation but was resistant to monkey stomach lavage fluid degradation, suggesting that native conformation was intact. Site-directed mutagenesis of the histidine residues of SEA was subsequently performed. SEA-H44A (SEA with histidine 44 replaced with alanine), SEA-H44D, SEA-H50A, SEA-H50D, SEA-H114A, SEA-H114D, SEA-H187A, and SEA-H187D retained superantigen and emetic activities, whereas SEA-H225A and SEA-H225D were defective in the ability to stimulate T-cell proliferation. These mutants were unable to compete with SEA for …

Association Between Nutrient Intake And Risk Of Coronary Heart Disease In California Seventh-Day Adventists, Peter Pribiš

Loma Linda University Electronic Theses, Dissertations & Projects

Dietary habits are believed to be major determinants of risk of coronary heart disease (CHD). Studies of high and low risk populations have suggested several nutrients to be protective or hazardous against CHD. Most published work since 1984 on diet and CHD in the Adventist Health Study (AHS) population examined either foods or food groups. This study investigated ten nutrients as exposure variables and their relationship to CHD risk. A cohort of 23,616 nonsmoking, non-Hispanic white California Seventh-day Adventists was followed for six years beginning in 1976. Extensive dietary information was collected at baseline, as well as values of traditional …

Cd40-Cd40 Ligand Interactions In Experimental Allergic Encephalomyelitis And Multiple Sclerosis., Koen Gerritse, Jon D. Laman, Randolph J. Noelle, Alejandro Aruffo

Dartmouth Scholarship

We investigated the role of CD40-CD40 ligand (CD40L) interactions in multiple sclerosis (MS) and experimental allergic encephalomyelitis (EAE). Activated helper T cells expressing CD40L (gp39) surface protein were found in MS patient brain sections, but not in brain tissue sections of normal controls or patients with other neurological disease. CD40L-positive cells were co-localized with CD40-bearing cells in active lesions (perivascular infiltrates). Most of these CD40-bearing cells proved to be of the monocytic lineage (macrophages or microglial cells), and relatively few were B cells. To functionally evaluate CD40-CD40L interactions, EAE was elicited in mice by means of proteolipid-peptide immunization. Treatment with …

Antisense Oligodeoxynucleotide Inhibition Of A Swelling-Activated Cation Channel In Osteoblast-Like Osteosarcoma Cells., Randall L. Duncan, Neil Kizer, Elizabeth L. Barry, Peter P A Friedman, Keith A. Hruska

Dartmouth Scholarship

By patch-clamp analysis, we have shown that chronic, intermittent mechanical strain (CMS) increases the activity of stretch-activated cation channels of osteoblast-like UMR-106.01 cells. CMS also produces a swelling-activated whole-cell conductance (Gm) regulated by varying strain levels. We questioned whether the swelling-activated conductance was produced by stretch-activated cation channel activity. We have identified a gene involved in the increase in conductance by using antisense oligodeoxynucleotides (ODN) derived from the alpha 1-subunit genes of calcium channels found in UMR-106.01 cells (alpha1S, alpha1C, and alpha1D). We demonstrate that alpha 1C antisense ODNs abolish the increase in Gm in response to hypotonic swelling following …

Serum Antibody Titres Against Canine Parvovirus Among Canine Pups After The First Vaccination Of Cpv Vaccine(ระดับภูมิคุ้มกันต่อโรคลำไส้อักเสบติดต่อจากเชื้อพาร์โวไวรัสภายหลังการฉีดวัคซีนเข็มแรกในสุนัข), Weerapon Haemrattanakorn, Jessada Chanpaiboon, Kanisak Oraveerakul, Suwit Kumthorntip

The Thai Journal of Veterinary Medicine

Serum antibody titers against Canine Parvovirus (CPV) in puppies after the first CPV vaccination were studied. Total 58 serum samples were obtained from Small Animal Hospital, Faculty of Veterinary Science, Chulalongkorn University. Results revealed by Hemagglutination Inhibition test showed that the puppies had HI-titers between 320-20,480 with Geometric Mean Titer (GMT) 2,717.6. In addition, another set of 56 samples obtained from puppies in Royal Thai Army Military Working Dog Center and private kennels before and 7, 14-day-post vaccination were included. It was appeared that titers ranged from 640-5,120, 320-2,560 and 320-5,120 with GMT 1,413.3, 1,203.1 and 1,280 in samples from …

Endogenous Amino Acid Losses In Pigs And Poultry: A Review(การสูญเสียกรดอะมิโนภายในระบบทางเดินอาหารของไก่และสุกร : บทความ), Kris Angkanaporn, Wayne L. Bryden, Velmurugu Ravindran

The Thai Journal of Veterinary Medicine

Considerable quantities of non-dietary protein are secreted into the digesta of pigs and poultry. This protein is defined as the protein found in the chyme or faeces when a nitrogen-free diet is fed to animals. Endogenous proteins comprise protein, peptides, amino acids and nitrogen-containing substances from saliva, bile, pancreatic, gastric and intestinal secretions; plasma and sloughed epithelial cells. These endogenous proteins are digested and absorbed in a manner similar to those of dietary origin. Conventional approaches to the estimation of obligatory loss have included the measurement of amino acid excretion in fasted animals, in animals fed on protein-free diets, or …

The Dynamics Of Antibody Titres And Faecal Egg Output In Cattle And Buffalo Following Infection With 500 And 1000 Fasciola Gigantica Metacercariae(ความผันแปรของแอนติบอดี ไตเตอร์และปริมาณไข่พยาธิใบไม้ตับ ในอุจจาระของโคและกระบือที่ได้รับเชื้อ 500 และ 1000 เมทต้าเซอร์คาเรีย), Piyanoot Prasitirat, Suree Thammasart, Tasanee Chompoochan, Suwannee Nithiuthai, Noriyuki Taira

The Thai Journal of Veterinary Medicine

Infection with the fluke Fasciola gigantica was detected by the ELISA and the beads techniques and used to determine the antibody titre and egg count per gram (epg) in cattle and buffalo respectively. Each group of 4 cattle and 4 buffalo were infected with 500 and 1000 metacercariae. The experiments were carried out over a period of 28 weeks with sampling taking place every 2-4 weeks. Using the ELISA test, the results revealed, that the patterns of antibody titres against F. gigantica in infected cattle and buffalo, were similar. The earliest ELISA titre detection of 500 metacercariae infected cattle was …

What's Happening: February 21, 1996, Maine Medical Center

What's Happening

No abstract provided.

Coated-Products With Potent Anti-Hiv And Antimicrobial Properties, George Digenis, Alexander G. Digenis

Pharmaceutical Sciences Faculty Patents

A composition which has potent anti-HIV activity. The composition is a high energy coprecipitate of nonoxynol-9 oligomers, polyvinyl-pyrrolidone (PVP) and iodine or PVP-I and shows a pronounced synergistic, anti-HIV effect between the compounds of the composition. The invention also includes paper and plastic products coated with the composition of the invention.

2,4-Disulfonyl Phenyl Butyl Nitrone, Its Salt And Their Use As Pharmaceuticals Free Radical Traps, John M. Carney

Pharmacology and Nutritional Sciences Faculty Patents

2,4-disulfonyl α-phenyl-tert-butyl nitrone and its pharmaceutically acceptable salts are disclosed. These materials are useful as pharmaceutical agents for oral or intravenous administration to patients suffering from acute central nervous system oxidation as occurs in a stroke or from gradual central nervous system oxidation which can exhibit itself as progressive central nervous system function loss.

What's Happening: January 10, 1996, Maine Medical Center

What's Happening

No abstract provided.

Why Do We Worry About Trace Poisons?, Allan Mazur

RISK: Health, Safety & Environment (1990-2002)

Dr. Mazur relates how protests by the political left against nuclear tests and by the political right against fluoridation set the stage for Silent Spring to move the public toward being concerned about latent risks.

Photochemotherapy Of Vascular Cells With 8-Methoxypsoralen And Visible Light: A Novel Approach To The Prevention Of Post-Angioplasty Restenosis, David Marshall Lee

Yale Medicine Thesis Digital Library

No abstract provided.

Use Of The (3)H-Tetracycline Rat Model In The Study Of Skeletal Compartmentalization And Metabolism Of Calcium In The Maturing Male And Female Rat And The Role Of The Skeleton In Calcium Homeostasis In The Adolescent Male Rat, Darrin Lee Demoss

Theses, Dissertations and Capstones

The (3)H-tetracycline bone labeling procedure was employed to monitor bone resorption from urinary loss curves in male and female rats of various ages. In addition, whole body dry skeletal mass and the loss of (3)H-tetracycline from individual bones was determined. It was found that the dry skeletal mass/body mass ratio of 24-week-old females was 30-40% greater than that for males. The urinary loss of (3)H-tetracycline was described by a double exponential equation indicating the presence of two distinct and independent exchangeable bone compartments. Both compartments decrease in size with age, but their label loss activities were different. The label loss …

Regulation Of Melanogenesis In B16 Mouse Melanoma Cells By Protein Kinase C (Pkc), Harish Mahalingam

Theses, Dissertations and Capstones

The pigment cell-specific expression of tyrosinase and TRP1 has been shown to be important for the production of melanin in pigmented cells. Using a pigmented cell line, B16 mouse melanoma, we obtained evidence that PKC plays a major role in regulating melanogenesis. Chronic treatment with phorbol dibutyrate (PDBu) leads to downregulation of PKC activity and protein levels. This is accompanied by a loss of pigmentation which is correlated with a 50% reduction and a complete loss of TRP1 and tyrosinase respectively. Similar results were obtained with Northern and Western blotting indicating that PKC may regulate the steady state levels of …

Analysis Of Target Cell Susceptibility As A Basis For The Development Of A Chemoprotective Strategy Against Benzene-Induced Hematotoxicities, M. A. Trush, L. E. Twerdok, S. J. Rembish, H. Zhu, Y. Li

Osteopathic Medicine, Jerry M. Wallace School of

No abstract provided.

Truncated Enterohemorrhagic Escherichia Coli (Ehec) O157:H7 Intimin (Eaea) Fusion Proteins Promote Adherence Of Ehec Strains To Hep-2 Cells, Marian L. Mckee, Alison D. O'Brien

Uniformed Services University of the Health Sciences

Intimin, the product of the eaeA gene in enterohemorrhagic Escherichia coli O157:H7 (EHEC), is required for intimate adherence of these organisms to tissue culture cells and formation of the attaching and effacing lesion in the gnotobiotic pig. Because of the importance of intimin in the pathogenesis of EHEC O157:H7 infection in this animal model, we began a structure-function analysis of EaeA. For this purpose, we constructed amino-terminal fusions of the intimin protein with six histidine residues to form two independent fusions. The longer fusion, RIHisEae, contained 900 of the 935 predicted amino acids and included all but the extreme amino …

Dentalgram - Vol. 10, No. 01, Loma Linda University School Of Dentistry

Dentalgram

In this issue:

• Clinic 120
• Summer mission
• Cart disinfection
• New faculty
• In memoriam
• Rideshare social
• Christmas breakfast
• Norton clean-up
• Work bee
• Mission opportunities