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Study Of The Hiv-1 Gene Regulatory Proteins Tat And Rev By The Two-Hybrid System In Yeast, David Allen Elkins

Loma Linda University Electronic Theses, Dissertations & Projects

Tat and Rev, small proteins encoded by the Human Immunodeficiency Virus type 1 (HIV-1), regulate its pattern of gene expression within infected cells. Tat increases transcription from the integrated pro virus by approximately a thousand-fold; Rev effects a shift in the pattern of splicing of the viral mRNA. Both are likely to interact with cellular proteins in executing their respective functions. Tat has additional activities (including neurotoxicity and inhibition of lymphocyte activation) which may be extraneous to its viral functions.

In order to identify cellular proteins interacting with Tat and Rev, the genes for both were cloned into plasmid vectors …

Cellular Responses In Escherichia Coli To Lethal And Sublethal Doses Of Ozone, Indira Ruth Komanapalli

Loma Linda University Electronic Theses, Dissertations & Projects

Ozone is a major component of photochemical smog. High levels of this pollutant, sufficient to affect human health are found in many urban areas worldwide. Though limited studies in humans are supported by extensive findings from animal experiments, a difficulty in interpreting the results of these experiments has lead to an ambiguity on the biochemical mechanism of ozone toxicity. To elucidate the mechanism by which ozone causes cell damage and eventual cell death we conducted a comprehensive study using Escherichia coli K-12 as a model.

Studies on the comparative inactivation of bacteriophage lambda (λ), Escherichia coli, and Candida albicans …

Dentalgram - Vol. 11, No. 06, Loma Linda University School Of Dentistry

Dentalgram

In this issue:

• Commencement ceremonies
• Awards heaped on students and faculty
• New DSA officers
• Dean's list
• Student news
• Faculty research presented
• Staff news
• Class of 2018 visits
• Glenn Curtis remembered
• LLU's Colombian sister school

Anthropogenic Electromagnetic Fields And Cancer: A Perspective, Charles Tomljanovic, Maxine Wright-Walters, Jules Stephensky

RISK: Health, Safety & Environment (1990-2002)

The authors review findings of a recent National Research Council report and conclude that, e.g., until a cancer promotion model can be identified for effective testing, the EMF issue will remain open to debate.

Chronicity In Strongyloides Stercoralis Infections: Dichotomy Of The Protective Immune Response To Infective And Autoinfective Larvae In A Mouse Model., R. A. Brigandi, H. L. Rotman, T. J. Nolan, G. A. Schad, D. Abraham

Department of Microbiology and Immunology Faculty Papers

Strongyloidiasis is an intestinal disease that can last for decades due to the occurrence of autoinfective larvae (L3a) in an infected person, which contribute to the maintenance of the population of adult worms in the intestine. The goal of the present study was to determine if L3a are susceptible to the protective immunity that targets the infective stage of the worm, the third-stage larvae (L3). Mice immunized and challenged with Strongyloides stercoralis L3 kill more than 90% of challenge larvae contained within diffusion chambers. The L3 do not remain antigenically static in mice, however, but undergo some degree of antigenic …

Distribution Of Endosomal, Lysosomal And Mhc Markers In A Monocytic Cell Line Infected With Chlamydia Psittaci, David M. Ojcius, Raymond Hellio, Alice Dautry-Varsat

All Dugoni School of Dentistry Faculty Articles

The intracellular fate of Chlamydia psittaci during infection of a monocytic cell line, THP1, was characterized. Cytochalasin D inhibited phagocytosis of latex beads but had no effect on infection by C. psittaci, and vacuoles expressed the transferrin receptor, suggesting accessibility to the endocytic pathway. Early Chlamydia-containing vacuoles expressed major histocompatibility complex (MHC) class I molecules, and most vacuoles fused with host cell lysosomes, since they expressed LAMP-1 and had acidic pHs. In cells prestimulated with gamma interferon, vacuoles also expressed MHC class II molecules, suggesting that the monocytes might effectively process Chlamydia-derived antigens for presentation by MHC class I and …

What's Happening: May 28, 1997, Maine Medical Center

What's Happening

No abstract provided.

Density, Distribution, And Habitat Of Flammulated Owls In Idaho, Craig Groves, Terry Frederick, Glenn Frederick, Eric Atkinson, Melonie Atkinson, Jay Shepherd, Gregg Servheen

Great Basin Naturalist

From 1990 to 1992 we surveyed for Flammulated Owls (Otus flammeolus) in 3 areas in Idaho: Salmon National Forest (SNF), Payette National Forest and adjacent Hells Canyon National Recreation Area (PNF-HCNRA), and Nez Perce National Forest (NPNF). We also collected and summarized information on all historic and modern records of Flammulated Owls in Idaho. Flammulated Owls were detected on 65% of 68 routes (2–16 km in length) surveyed at densities ranging from 0.04 to 1.25 singing males/40 ha. Owls were detected on survey routes as early as 10 May and as late as 23 July. Mean percent canopy …

Erratum

Great Basin Naturalist

No abstract provided.

Diplostomiasis In Native And Introduced Fishes From Yellowstone Lake, Wyoming, Victor H. Inchausty, Michael Foutz, Richard A. Heckmann, Claudete Ruas, Paulo Ruas

Great Basin Naturalist

Totals of 101 native Yellowstone cutthroat (Oncorhynchus clarki bouvieri), 27 introduced lake trout (Salvelinus namaycush), and 40 introduced longnose sucker (Catostomus catostomus) from Yellowstone Lake, Wyoming, USA, were examined for eye flukes. Metacercariae of the trematode fluke Diplostomum were in vitreous humor and/or lens of 94% of Yellowstone cutthroat trout, 92% of lake trout, and 78% of longnose sucker. Longnose sucker had 7% prevalence of infection in both lens and vitreous humor of metacercariae, while Yellowstone cutthroat trout had 3% and lake trout 8%. Diplostomum spathaceum was in lens tissue of 5% of infected …

Catholic Identity In Health Care And The Relevance Of The 1994 Ethical And Religious Directives For Catholic Health Care Services, Germain Kopaczynski

The Linacre Quarterly

No abstract provided.

Dentalgram - Vol. 11, No. 05, Loma Linda University School Of Dentistry

Dentalgram

In this issue:

• Accolades to LLUSD Students
• Dr. Boyne Publishes Book
• Clinic with a Heart
• Biocompatibility Specialist Joins LLUSD
• Dr. Crawford Visits Africa
• Class of 2000 Statistics
• Millennial Class Named
• Volunteerism
• Employee Appreciation
• CPR Renewals
• Birthdays

Fetal Alcohol Syndrome: Information And Intervention Strategies For School, Home, And Therapeutical Settings, Mindy Dawn Anderson

Undergraduate Honors Capstone Projects

I was introduced into the world of Fetal Alcohol Syndrome winter quarter, 1996. My goals for that year included researching this topic in order to understand what it was physically, behaviorally, and on a cognitive level. My assignment involved writing a research paper around those characteristics, along with the development of their speech and language, presenting my findings to my classmates, and competing at the USU Scholar's Day competition in May of 1996. I received 2nd place at this competition. During the summer of that year, I researched FAS more to receive the needed resources to compile an annotated bibliography …

Human Immunodeficiency Virus Type 1 Infection Of Cells And Tissues From The Upper And Lower Human Female Reproductive Tract, Alexandra L. Howell, Robert D. Edkins, Sherry E. Rier, Grant R. Yeaman, Judy E. Stern, Michael W. Fanger, Charles R. Wira

Dartmouth Scholarship

Viable tissue sections and isolated cell cultures from the human fallopian tube, uterus, cervix, and vaginal mucosa were examined for susceptibility to infection with human immunodeficiency virus type 1 (HIV-1). We examined infectivity by using the monocytotropic strain HIV-1_JR-FLand several primary isolates of HIV-1 obtained from infected neonates. HIV-1 infection was measured by p24 production in short-term culture and by immunofluorescence detection of HIV-1 Nef and p24 proteins by laser scanning confocal microscopy. Three-color immunofluorescence was used to phenotype HIV-infected cells within tissue sections from each site. Our findings indicate that epithelial, stromal, and dendritic cells and cells …

Fetal Alcohol Syndrome: Information And Intervention Strategies For School, Home, And Therapeutical Settings, Mindy Dawn Anderson

Undergraduate Honors Capstone Projects

I was introduced into the world of Fetal Alcohol Syndrome winter quarter, 1996. My goals for that year included researching this topic in order to understand what it was physically, behaviorally, and on a cognitive level. My assignment involved writing a research paper around those characteristics, along with the development of their speech and language, presenting my findings to my classmates, and competing at the USU Scholar's Day competition in May of 1996. I received 2nd place at this competition. During the summer of that year, I researched FAS more to receive the needed resources to compile an annotated bibliography …

Characterization Of Angiotensin Ii Receptor Subtypes In The Brain, David L. Saylor

Electronic Theses and Dissertations

The present studies explore binding, distribution, and function of angiotensin II (AII) receptors (AT$\sb1$ and AT$\sb2)$ in the brain. The discovery that sulfhydryl reducing agents masked some but not all AII receptors in the brain prompts an evaluation of commonly used binding assay buffer constituents. EDTA enhances binding (40%) at both AT$\sb1$ and AT$\sb2$ nuclei, while bacitracin did not alter binding at either receptor subtype. Phenanthroline and BSA differentially altered binding at AT1 (220% of control) and AT$\sb2$ (118% of control) receptors. The results indicate that phenanthroline and BSA would be poor buffer constituents for studies comparing binding at AT$\sb1$ …

Dna-Protein Cross-Linking By Pyrrolizidine Alkaloids, Gail L. Drew

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Pyrrolizidine alkaloids (PAs) are natural plant compounds found in hundreds of plant species worldwide and are reported to have cytotoxic, carcinogenic, antimitotic, and genotoxic activity. PAs are metabolized by the cytochrome P450 (CYP) system to the pyrrole or the N-oxide form. The pyrroles are bifunctional electrophilic alkylators that bind cellular nucleophiles such as DNA and proteins and sirupt normal cell processes, including DNA replication and gene transcription and can cause megalocytosis. The pyrroles dehydrosenecionine (DHSN) and dehydromonocrotaline (DHMO) are among the most potent PA cross-linkers and inducers of megalocytosis. DHSN and DHMO-induced cross-links in cultured normal (MDBK) and neoplastic (MCF7) …

Aflatoxin B1 Metabolism In Mammalian Pulmonary Tissue, J. Michael Eichelberger

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Aflatoxin B₁ (AFB₁) is a potent dietary hepatocarcinogen and may be a lung carcinogen when inhaled. To study the relative ability of lung and liver to metabolize AFB₁, a susceptible (Swiss-Webster rat) and resistant species (Syrian golden hamster) were pretreated with inducing agents in order to identify specific AFB₁ metabolizing enzymes in each tissue.

Analysis of AFB₁-exo-epoxide (AFBO) formation, O-dealkynation assays, and protein immunoblots demonstrated that cytochrome P450 (CYP) 1A proteins were overexpressed in both the lung and liver of hamsters pretreated with 3-methylchyolanthrene (3-MC). Only CYP1A1 was expressed in the lung …

Spin Trapping Pharmaceutical Compositions And Methods For Use Thereof, John M. Carney, Robert A. Floyd

Pharmacology and Nutritional Sciences Faculty Patents

Spin trapping compositions in general have now been discovered to be effective in treating a variety of disorders, including disorders such as those arising from ischemia, infection, inflammation, exposure to radiation or cytotoxic compounds, not just of the central and peripheral nervous systems but of peripheral organ disease having a wide variety of etiologies. In the preferred embodiment, the compositions for treating tissue damage from ischemia contain PBN, or active derivatives thereof, in a suitable pharmaceutical carrier for intravenous, oral, topical, or nasal/pulmonary administration. Many different disorders can be treated using these compounds, including diseases or disorders of the central …

Alterations In Human B Cell Calcium Homeostasis By Polycyclic Aromatic Hydrocarbons: Possible Associations With Cytochrome P450 Metabolism And Increased Tyrosine Phosphorylation, Barbara J. Mounho

Pharmaceutical Sciences ETDs

Polycyclic aromatic hydrocarbons (PAHs), are known immunotoxicants in animals, and are suspect toxins to the human immune system. The mechanism(s), however, by which PAHs exert immunosuppression have not been fully elucidated. Previous studies conducted in our laboratory have shown that PAHs, such as 7,12-dimethylbenz(a)- anthracene (DMB A) and benzo(a)pyrene (BaP) may exert their immunotoxic effects by altering intracellular calcium (Ca2+) homeostasis in lymphocytes. Intracellular Ca2+ is an important second messenger in the immune response, and the mobilization of Ca2+ is critical in the transduction of intracellular signals from the plasma membrane to the nucleus. The overall objective of this project …

Changes In Ankle Joint Proprioception Resulting From Strips Of Athletic Tape Applied Over The Skin, Guy G. Simoneau, Rebecca M. Degner, Cindi A. Kramper, Kent H. Kittleson

Physical Therapy Faculty Research and Publications

Athletic trainers, physical therapists, and other rehabilitation professionals stress the importance of proprioceptive reeducation during the rehabilitation process following an injury." 2 It is believed that an injury such as an inversion ankle sprain, for example, results in a reduction of proprioceptive function that may lead to future reinjuries. 3 One suggested palliative method to supplement a deficit in proprioceptive function at the ankle is the use of external support such as taping and braces.47 The tape or brace is believed to provide increased mechanical support as well as increased proprioception.

A Correlational Study Of Infertility And Marital Satisfaction, Charles H. Mccomb Jr.

Theses

This study examined the correlation between infertility and marital satisfaction. Thirty-six subject participated in the study. The participants were recruited from a support group. Other recruits came from friends. The survey was sent, completed, and returned by mail as to retain anonymity. The subjects came from St. Louis, MO. Most of the individuals in the study were white, Non-Hispanic, middle class, and college graduates. Each individual completed a demographic questionnaire and the Kansas Marital Satisfaction Scale. The compiled results found that there is a significant relationship between gender and marital satisfaction in a group of individuals with fertility problems. Another …

Sch 48973: A Potent, Broad-Spectrum, Antienterovirus Compound., Peter J. Buontempo, Stuart Cox, Jacquelyn Wright-Minogue, Jason L. Demartino, Angela M. Skelton, Eric Ferrari, Randi Albin, Edward J. Rozhon, V. Girijavallabhan, John F. Modlin, John F. O'Connell

Dartmouth Scholarship

SCH 48973 is a novel molecule with potent, selective, antienterovirus activity. In assays of the cytopathic effect against five picornaviruses, SCH 48973 had antiviral activity (50% inhibitory concentrations [IC50s]) of 0.02 to 0.11 microg/ml, with no detectable cytotoxicity at 50 microg/ml. SCH 48973 inhibited 80% of 154 recent human enterovirus isolates at an IC50 of 0.9 microg/ml. The antiviral activity of SCH 48973 is derived from its specific interaction with viral capsid, as confirmed by competition binding studies. The affinity constant (Ki) for SCH 48973 binding to poliovirus was 8.85 x 10(-8) M. In kinetic studies, a maximum of approximately …

What's Happening: March 19, 1997, Maine Medical Center

What's Happening

No abstract provided.

Anti-Idiotype Monoclonal Antibody 1a7 And Use For The Treatment Of Melanoma And Small Cell Carcinoma, Malaya Chatterjee, Kenneth A. Foon

Microbiology, Immunology and Molecular Genetics Faculty Patents

The present invention relates isolation of anti-idiotypic antibody 1A7 raised against anti-GD2 mAb 14G2a and its use for the treatment of melanoma and small cell carcinoma. The antibody may be used as a substitute for isolated purified GD2 antigen in any appropriate application.

Ureteroscopic Lithotripsy., Demetrius H. Bagley

Department of Urology Faculty Papers

There is a wide array of endoscopic lithotriptors presently available. Each of these has its own advantages and disadvantages. No single lithotriptor is suitable for all applications and none can meet the goal of fragmenting all calculi while remaining harmless to tissue.

Neuroprotective Action Of Cycloheximide Involves Induction Of Bcl-2 And Antioxidant Pathways, Katsutoshi Furukawa, Steven Estus, Weiming Fu, Robert J. Mark, Mark P. Mattson

Sanders-Brown Center on Aging Faculty Publications

The ability of the protein synthesis inhibitor cycloheximide (CHX) to prevent neuronal death in different paradigms has been interpreted to indicate that the cell death process requires synthesis of “killer” proteins. On the other hand, data indicate that neurotrophic factors protect neurons in the same death paradigms by inducing expression of neuroprotective gene products. We now provide evidence that in embryonic rat hippocampal cell cultures, CHX protects neurons against oxidative insults by a mechanism involving induction of neuroprotective gene products including the antiapoptotic gene bcl-2 and antioxidant enzymes. Neuronal survival after exposure to glutamate, FeSO₄, and amyloid β-peptide …

Individual Differences In Response To Novelty, Amphetamine-Induced Activity And Drug Discrimination In Rats, Rick A. Bevins, J. E. Klebaur, M. T. Bardo

Department of Psychology: Faculty Publications

Rats mere pre-tested in several individual difference screens - novelty-induced activity, novelty-induced place preference, novel-object interaction, and amphetamine-induced activity. Rats that were more sensitive to the locomotor effects of amphetamine were more active in an inescapable novel environment and displayed a greater preference for a novel environment. All animals were then trained to discriminate amphetamine (1 mg/kg) from saline in a two-bar discrimination procedure using food-maintained responding. After acquisition of the discrimination (mean =37 trials), two amphetamine generalization tests (0.0625,0.125,0.25,0.5, 1.0 and 2.0 mg/kg) were conducted. In the second generalization test, rats that were more sensitive to the activating effect …

Converging Evidence For One-Trial Context Fear Conditioning With An Immediate Shock: Importance Of Shock Potency, Rick A. Bevins, Janice E. Mcphee, Anthony S. Rauhut, John J.B. Ayres

Department of Psychology: Faculty Publications

In a sample of 208 Holtzman-descended albino rats, we found evidence with 4 measures of conditioning (freezing, defecation, side crossing, and nose crossing) that a single 2-s, 1.0-mA immediate shock could condition fear to a context (Experiments 1, 2, and 4). When we reduced the shock intensity to 0.5 mA, we obtained a complete immediate-shock conditioning deficit according to all measures in Experiment 3 and to all but the defecation measure in Experiment 4. Results suggest two conclusions: (a) Differences in shock potency between laboratories may help explain discrepant findings about whether immediate shock supports contextual conditioning; (b) theories of …

Natural Variability Of Vegetation, Soils, And Physiography In The Bristlecone Pine Forest Of The Rocky Mountains, Brigitte M. Ranne, William L. Baker, Tom Andrews, Michael G. Ryan

Great Basin Naturalist

Pinus aristata Engelm. forest vegetation in Colorado was studied to determine vegetation composition and the relationship between vegetation and environment. Species percent cover, tree size class, and environmental variables were recorded for 49 plots. Previously collected data for 4 plots from New Mexico were included. Environmental variables included latitude, longitude, elevation, slope, aspect, topographic position, parent material, percent rock cover, mean rock size, litter depth, estimated plot age class, and evidence of anthropogenic disturbance. Soils were analyzed for texture, depth, and percent carbon and nitrogen. Pinus aristata foliage was analyzed for percent nitrogen and phosphorus. Direct and indirect gradient analyses …