Digital Commons Network^™

[Book Review Of] Euthanasia Examined, John Keown, Ed., John Hartley

The Linacre Quarterly

No abstract provided.

Expression Of Protein Kinase C Beta In The Heart Causes Hypertrophy In Adult Mice And Sudden Death In Neonates, Joel C. Bowman, Susan F. Steinberg, Tiangrong Jiang, David L. Geenen, Glenn I. Fishman, Peter M. Buttrick

Peer Reviewed Articles

Protein kinase C (PKC) activation in the heart has been linked to a hypertrophic phenotype and to processes that influence contractile function. To establish whether PKC activation is sufficient to induce an abnormal phenotype, PKCbeta was conditionally expressed in cardiomyocytes of transgenic mice. Transgene expression in adults caused mild and progressive ventricular hypertrophy associated with impaired diastolic relaxation, whereas expression in newborns caused sudden death associated with marked abnormalities in the regulation of intracellular calcium. Thus, the PKC signaling pathway in cardiocytes has different effects depending on the timing of expression and, in the adult, is sufficient to induce pathologic …

Modulation Of The Rat Potassium Channel Kv1.5, Daniel Benjamin Saal

Yale Medicine Thesis Digital Library

1. Kv1.5 is a delayed rectifier-type potassium channel. We have examined the localization of the channel protein using a polyclonal antibody against Kv1.5. This channel is expressed in numerous regions of the brain, primarily in glial cells. Its expression is particularly intense in the endfoot processes which abut the microvasculature of the brain. It is also expressed in the cell bodies and cellular processes in the pituitary.2. Immunoprecipitation of the Kv1.5 protein, from HEK 293 cells which were transformed with the Kv1.5 gene, revealed that the channel is phosphorylated and that treatment of cells with dibutyryl cyclic AMP (dibcAMP) or …

Role Of Egr-1 Gene Expression In B Cell Receptor-Induced Apoptosis In An Immature B Cell Lymphoma, Subramanian Muthukkumar, Seong-Su Han, Sumathi Muthukkumar, Vivek M. Rangnekar, Subbarao Bondada

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Ligation of B cell receptor (BCR) on BKS-2, an immature B cell lymphoma by anti-IgM antibodies (Ab) caused apoptosis. Here we report that signaling through B cell receptor in wild type BKS-2 cells down-regulated the expression of Egr-1, a zinc finger-containing transcription factor. A reduction in the level ofEgr-1 mRNA could be demonstrated as early as 30 min after the ligation of BCR on BKS-2 cells. Immunocytochemical and Western blot analysis revealed that the expression of EGR-1 protein was also inhibited by anti-IgM treatment. Antisense oligonucleotides to Egr-1 caused growth inhibition and apoptosis in BKS-2 cells, suggesting that …

Cross-Validation Of The Injury Behavior Checklist In A School-Age Sample, Richard Potts, Isaac Martinez, Angela Dedmon, Leslie Schwarz, David Dilillo, Lisa Swisher

Department of Psychology: Faculty Publications

Examined descriptive characteristics, internal validity, and convergent validity of the Injury Behavior Checklist (IBC) in a sample of 7- to 10-year-old children. Although the IBC was originally designed for use with preschool children, results of the present study showed that it has acceptable psychometric qualities for use with children as old as 9 years. The IBC shows promise as an easily administered instrument for research on psychological and behavioral mechanisms of child hood injury, as well as for individual screening for injury liability.

Permeable, Non-Irritating Prodrugs Of Nonsteroidal And Steroidal Agents, Paul Ashton, Thomas J. Smith, Peter G. Glavinos, John D. Conklin, Peter A. Crooks, Robert M. Riggs, Tadeusz Cynkowski, Grazyna Cynkowska

Ophthalmology and Visual Science Faculty Patents

Prodrugs containing an active drug molecule linked to a polyethylene glycol group, and a method of use thereof are described. Exemplary soluble ester prodrugs contain naproxen, triamcinolone acetonide, gancyclovir, taxol, cyclosporin, dideoxyinosine, trihydroxy steroids, and flurbiprofen molecules linked to polyethylene glycol (PEG) groups. Pharmaceutical compositions containing these prodrugs, and a method of using these esters for treating disease states or symptoms are also described.

Spin Trapping Compounds, John M. Carney, Robert A. Floyd

Pharmacology and Nutritional Sciences Faculty Patents

Compositions containing as the active ingredient a spin-trapping reagent, preferably α-phenyl butyl nitrone (PBN) or spin-trapping derivatives thereof, in a suitable pharmaceutical carrier for administration to a patient are disclosed for treating or preventing symptoms associated with aging or other conditions associated with oxidative tissue damage. Other spin-trapping agents can also be used, such as 5,5-dimethyl pyrroline N-oxide (DMPO) or α-(4-pyridyl 1-oxide)-N-tert-butylnitrone (POBN), and other spin-trapping derivatives thereof. These compositions and methods are useful in the treatment of age-related disorders, pre-surgical and/or pre-anesthetic preparation or administration of chemotherapeutic agents, and in the treatment of disorders or trauma of the brain, …

Detection And Characterization Of Sp1 Binding Activity In Human Chondrocytes And Its Alterations During Chondrocyte Dedifferentiation., Rita M. Dharmavaram, Gang Liu, Sheryl D. Mowers, Sergio A. Jimenez

Department of Medicine Faculty Papers

We have detected DNA binding activity for a synthetic oligonucleotide containing an Sp1 consensus sequence in nuclear extracts from human chondrocytes. Changes in the levels of Sp1 oligonucleotide binding activity were examined in nuclear extracts from freshly isolated human chondrocytes, from chondrocytes that had been cultured under conditions that allowed the maintenance of a chondrocyte-specific phenotype on plastic dishes coated with the hydrogel poly(2-hydroxyethyl methacrylate), and from chondrocytes induced to dedifferentiate into fibroblast-like cells by passage in monolayer culture on plastic substrata. It was observed that Sp1 binding was 2-3-fold greater in nuclear extracts from dedifferentiated chondrocytes than in nuclear …

The Leader Who Serves (Scottsdale, Az), C. William Pollard

C. William Pollard Papers

In this address to the Baptist Healthcare Association (Scottsdale, AZ), Pollard applauds religious healthcare institutions for their commitment to the physical and spiritual welfare of the human person. With this holistic approach, they refuse to submit to the logic of simple economic efficiency and thus serve as a prime example of servant leadership.

Chronic Hypothermia And Energy Expenditure In A Neurodevelopmentally Disabled Patient: A Case Study, Jane M. Gervasio, Roland N. Dickerson, Rex O. Brown, J. Barret Matthews

Scholarship and Professional Work – COPHS

Hypothermia is defined as a core body temperature of <35°C and results in a decrease in measured resting energy expenditure. A 51-year-old mentally disabled patient experienced chronic hypothermia from neurologic sequelae. Because of her continued weight gain and increased body fat in the presence of presumed hypocaloric nutrition, indirect calorimetry measurements were performed twice in a 3-month period. The resting energy expenditure measurements prompted a reduction of her daily caloric intake to prevent further overfeeding. Hypothermia reduces oxygen consumption and, as a consequence, decreases resting energy expenditure. In patients for whom chronic hypothermia is a problem, nutritional intake must be adjusted to prevent overfeeding, excessive weight gain, and the long-term complications of an excess of total calories.

The Effect Of Self-Focused Attention On Psychopathology, Keith Payne

Mahurin Honors College Capstone Experience/Thesis Projects

This study investigated whether self-focused attention is related to psychopathology in general or if, instead, specific types of self-focus are associated with specific disorders. Self-focus was measured as private self-consciousness, public self-consciousness, emotional self-reference, or performance self-reference. Personality measures included depression, phobias, 'health concern, obsessiveness, and disordered eating. Public self-consciousness was related to greater depression, obsessiveness, and disordered eating. Private self-consciousness was positively correlated with depression and obsessiveness. Performance self-reference was related to lower levels of depression and obsessiveness. The findings indicate that different types of self-focused attention are involved in different disorders. Results are interpreted with reference to self-awareness …

Foundation News: Fall 1997, University Of Health Sciences Foundation

Foundation News (1997)

Contents: UHS LiFE Challenge -- Foundation Faces -- Foundation Grants -- UHS Scholarships -- Gift Opportunities -- Foundation Activities -- UHS Foundation Board.

Triple Dissociation Of Anterior Cingulate, Posterior Cingulate, And Medial Frontal Cortices On Visual Discrimination Tasks Using A Touchscreen Testing Procedure For The Rat., T J Bussey, J L Muir, B J Everitt, T W Robbins

Brain and Mind Institute Researchers' Publications

Four experiments examined effects of quinolinic acid-induced lesions of the anterior cingulate, posterior cingulate, and medial frontal cortices on tests of visual discrimination learning, using a new "touchscreen" testing method for rats. Anterior cingulate cortex lesions impaired acquisition of an 8-pair concurrent discrimination task, whereas posterior cingulate cortex lesions facilitated learning but selectively impaired the late stages of acquisition of a visuospatial conditional discrimination. Medial frontal cortex lesions selectively impaired reversal learning when stimuli were difficult to discriminate; lesions of anterior and posterior cingulate cortex had no effect. These results suggest roles for the anterior cingulate, posterior cingulate, and medial …

Nurses Alumni Association Bulletin, Fall 1997, Betty Piersol, Elizabeth K. Prisnock, Marian E. Smith, Margaret Summers, Sue Henrie-Strup, Maureen Mcvail

Nursing Alumni Bulletins

1997-1998 Meeting Dates Calendar

1998 Annual Luncheon-Meeting Notice

Officers and Committee Chairs

Bulletin Publication Committee

1997-1998 Meeting Dates Calendar

The President's Message

Treasurer's Report

Trivia and News About Our Graduates

Highlights of 1997

Keeping Kids Healthy After Hours

Scholarship Funds At Work

Tribute to Tanya

Medical Mission Outreach 1996

History Of The Jefferson Pin

Facts Not Fiction

Rib Ticklers

Mama Will Protect Me

Happy Birthday

Fiftieth Anniversary

Resume of Minutes of Alumni Association Meetings

Alumni Office News

Committee Reports

• Bulletin
• By-Laws
• Development
• Diploma School of Nursing Alumni Association-Mabel C. Prevost Scholarship Fund
• Nominating
• Relief Fund
• Satellite
• Social
• Scholarship

Luncheon Photos …

Identification Of Mycobacterium Paratuberculosis Gene Expression Signals, John P. Bannantine, Raul G. Barletta, Charles O. Thoen, Robert E. Andrews Jr.

School of Veterinary and Biomedical Sciences: Faculty Publications

Mycobacterium paratuberculosis promoter-containing clones were isolated from a genomic DNA library constructed in the transcriptional-translational fusion vector pYUB76. The promoter-containing DNA fragments were identified in the surrogate host Mycobacterium smegmatis by expression of the promoterless lacZ reporter gene of pYUB76. The expression signals exhibited a wide range of strengths, as indicated by their corresponding β-galactosidase activities. Eight clones were sequenced and characterized further. Predicted open reading frames and codon usage were identified by computer analysis. Database searching for related sequences using the BLAST method revealed no homologies. Transcriptional activity was measured by slot-blot hybridization with steady-state RNA isolated from lacZ …

Anticoagulation And Elective Surgery., J. Spandorfer, G. Merli

Department of Medicine Faculty Papers

No abstract provided.

Myelin-Associated Glycoprotein Interacts With Neurons Via A Sialic Acid Binding Site At Arg118 And A Distinct Neurite Inhibition Site, Song Tang, Ying Jing Shen, Maria Elena Debellard, Gitali Mukhopadhyay, James L. Salzer, Paul R. Crocker, Marie T. Filbin

Publications and Research

Inhibitory components in myelin are largely responsible for the lack of regeneration in the mammalian CNS. Myelin-associated glycoprotein (MAG), a sialic acid binding protein and a component of myelin, is a potent inhibitor of neurite outgrowth from a variety of neurons both in vitro and in vivo. Here, we show that MAG’s sialic acid binding site is distinct from its neurite inhibitory activity. Alone, sialic acid–dependent binding of MAG to neurons is insufficient to effect inhibition of axonal growth. Thus, while soluble MAG-Fc (MAG extracellular domain fused to Fc), a truncated form of MAG-Fc missing Ig-domains 4 and 5, MAG(d1-3)-Fc, …

What's Happening: September 17, 1997, Maine Medical Center

What's Happening

No abstract provided.

Metabolically Inactive Insulin Analog Prevents Type I Diabetes In Prediabetic Nod Mice, Dennis G. Karounos, J. Scott Bryson, D. A. Cohen

Internal Medicine Faculty Publications

The purpose of this study was to determine the relative importance of the metabolic effects of insulin for diabetes prevention by administering insulin or an inactive insulin analog by daily subcutaneous injections to prediabetic mice. A recombinant monomeric human insulin analog, which does not bind to the insulin receptor as a consequence of an alteration of a single amino acid at position 25 of the B chain, was shown to be equally effective at diabetes prevention as was intact insulin. In contrast to native insulin, the insulin analog did not cause hypoglycemia after subcutaneous injection. The insulin analog, however, protected …

Myocyte Apoptosis During Acute Myocardial Infarction In The Mouse Localizes To Hypoxic Regions But Occurs Independently Of P53, Shani Bialik, David Geenen, Isaac Sasson, Rendi Cheng, James Horner, Sydney Evans, Edith Lord, Cameron Koch, Richard Kitsis

Peer Reviewed Articles

Significant numbers of myocytes die by apoptosis during myocardial infarction. The molecular mechanism of this process, however, remains largely unexplored. To facilitate a molecular genetic analysis, we have developed a model of ischemia-induced cardiac myocyte apoptosis in the mouse. Surgical occlusion of the left coronary artery results in apoptosis, as indicated by the presence of nucleosome ladders and in situ DNA strand breaks. Apoptosis occurs mainly in cardiac myocytes, and is shown for the first time to be limited to hypoxic regions during acute infarction. Since hypoxia- induced apoptosis in other cell types is dependent on p53, and p53 is …

What's Happening: September 3, 1997, Maine Medical Center

What's Happening

No abstract provided.

Update - September 1997, Loma Linda University Center For Christian Bioethics

Update

In this issue:

-- Cocaine Addiction: The Nemesis of Modern Culture

Potential Role Of Tnfα And Lipoprotein Lipase As Candidate Genes For Obesity, Philip A. Kern

Clinical and Translational Science Faculty Publications

To maintain body weight, metabolic efficiency was promoted during evolution; two candidate genes for body weight regulation are lipoprotein lipase (LPL) and tumor necrosis factor-α [TNFα). Human fat cells do not synthesize lipid, but rely on LPL-mediated plasma triglyceride hydrolysis. Adipose LPL is elevated in obesity. Following weight loss, LPL is elevated further, suggesting attempts to maintain lipid stores during fasting and to replenish lipid stores during refeeding. Muscle LPL is regulated inversely to adipose LPL. Thus, an increased adipose/muscle LPL ratio would partition dietary lipid into adipose tissue and would explain some of the variability in weight gain when …

A Servey Of Dental Calculi And Dietary Factors In Dogs, Cholawat Pacharinsak, Damri Darawiroj, Jiranunt Jirasangthong

The Thai Journal of Veterinary Medicine

119 mongrel dogs, brought to Chulalongkorn Veterinary Teaching Hospital, were investigated over a 150-day period. Divided into 4 groups (less than 0.5 yrs, 0.5 to 1.49 yrs, 1.5 to 4.99 yrs, and more than 4.99 yrs) the age of the animals was related to the severity of the accumulated calculi (P<0.05), particularly in the area of the buccal surface. The group, fed on a soft diet, had more calculi deposits than those fed a hard one.

The Type 2 Iodothyronine Deiodinase Is Expressed Primarily In Glial Cells In The Neonatal Rat Brain, Ana Guadaño-Ferraz, Maria Jesus Obregón, Donald L. St. Germain, Juan Bernal

Dartmouth Scholarship

Thyroid hormone plays an essential role in mammalian brain maturation and function, in large part by regulating the expression of specific neuronal genes. In this tissue, the type 2 deiodinase (D2) appears to be essential for providing adequate levels of the active thyroid hormone 3,5,3′-triiodothyronine (T3) during the developmental period. We have studied the regional and cellular localization of D2 mRNA in the brain of 15-day-old neonatal rats. D2 is expressed in the cerebral cortex, olfactory bulb, hippocampus, caudate, thalamus, hypothalamus, and cerebellum and was absent from the white matter. At the cellular level, D2 is expressed predominantly, if not …

Recommendations For Elimination Of Bovine Tuberculosis In Free-Ranging White-Tailed Deer In Michigan, K. L. Cool, James Haveman, Dan Wyant

Michigan Bovine Tuberculosis Bibliography and Database

A significant infection rate of bovine TB in the deer population of the northeastern lower peninsula poses a potential risk to several important values including public health, United States Department of Agriculture (U.S.D.A.) TB-free accreditation for Michigan cattle, wildlife health, wildlife-related recreation and tourism and economic stability in several sectors. A risk assessment study by the U.S. D.A. Centers for Epidemiology and Animal Health (Fort Collins, CO) predicted that if no changes were made in the management of the affected free-ranging deer population, the TB prevalence (compared to the current prevalence of 2.3%). Although the current annual risk of TB …

Analysis Of Bovine Herpesvirus 1 Transcripts During A Primary Infection Of Trigeminal Ganglia Of Cattle, Luis M. Schang, Clinton J. Jones

School of Veterinary and Biomedical Sciences: Faculty Publications

During an infection of nonneuronal cells, bovine herpesvirus 1 (BHV-1) gene expression proceeds in a well-defined cascade. Products of immediate-early (IE) genes are expressed first, and they activate expression of early (E) and late (L) genes. Although the same cascade is assumed to occur during an infection of neurons in trigeminal ganglia (TG) of cattle, no experimental data is available to support this hypothesis. Consequently, we analyzed BHV-1 gene expression in bovine TG at 1, 2, 4, 7, and 15 days postinfection (dpi). Infectious virus was detected in ocular swabs from 1 to 7 dpi but not 15 dpi. By …

The Place Of Persons With Disabilities In Rotuman Society, Jan Rensel, Alan Howard

Pacific Studies

In Rotuma, as in many other small, face-to-face societies, persons with disabilities are treated not as members of categories based on their conditions, but as individuals having whole constellations of characteristics and interpersonal histories. Their roles are not scripted on the basis of disabilities, but patterned by a number of key cultural principles that guide all relationships, including notions of immanent justice for wrongdoing, expectations of generosity and reciprocity, personal autonomy, and concern for family honor. Case studies and analyses of exchange interactions are presented to illustrate these points.

Increased Focal Adhesion Kinase- And Urokinase-Type Plasminogen Activator Receptor-Associated Cell Signaling In Endothelial Cells Exposed To Asbestos, A Barchowsky, B M. Lannon, L C. Elmore, M D. Treadwell

Dartmouth Scholarship

Exposure of low-passage endothelial cells in culture to nonlethal amounts of asbestos, but not refractory ceramic fiber-1, increases cell motility and gene expression. These changes may be initiated by the fibers mimicking matrix proteins as ligands for receptors on the cell surface. In the present study, 1- to 3-hr exposures of endothelial cells to 5 mg/cm2 of chrysotile asbestos caused marked cell elongation and motility. However, little morphological change was seen when chrysotile was added to cells pretreated with either mannosamine to prevent assembly of glycophosphatidylinositol (GPI)-anchored receptors or with herbimycin A to inhibit tyrosine kinase activity. Affinity purification of …

Cell Signaling Pathways Elicited By Asbestos, B T. Mossman, S Faux, Y Janssen, L A. Jimenez, Cynthia Timblin, Christine Zanella, Jonathan Goldberg, Eric Walsh, Aaron Barchowsky, Kevin Driscoll

Dartmouth Scholarship

In recent years, it has become apparent that minerals can trigger alterations in gene expression by initiating signaling events upstream of gene transactivation. These cascades may be initiated at the cell surface after interaction of minerals with the plasma membrane either through receptorlike mechanisms or integrins. Alternatively, signaling pathways may be stimulated by active oxygen species generated both during phagocytosis of minerals and by redox reactions on the mineral surface. At least two signaling cascades linked to activation of transcription factors, i.e., DNA-binding proteins involved in modulating gene expression and DNA replication, are stimulated after exposure of lung cells to …