Digital Commons Network^™

Probable Renal-Limited Sarcoidosis Presenting With Tubulointerstitial Nephritis Without Granulomas On Renal Biopsy: A Diagnostic Challenge, Jawahar Hamood Alnoumani, Muhammad Shoaib, Asma Marzouqi, Ali Al Lawati

Sultan Qaboos University Medical Journal

Sarcoidosis is an idiopathic multisystem inflammatory disorder characterised by non-caseating granulomas and a wide range of reported prevalence. Renal-limited sarcoidosis is a rare manifestation of sarcoidosis which remains a diagnostic challenge due to lack of extra renal manifestation and specific diagnostic criteria. We report a 37-year-old man who presented to a tertiary care hospital, Muscat, Oman, in 2024, with a two-month history of fever, weight loss, hypercalcaemia, high-to-normal 1,25-Vitamin D, high angiotensin-converting enzyme levels and acute kidney injury. His renal biopsy showed evidence of tubulointerstitial nephritis without granulomas or nephrocalcinosis. After exclusion of infectious, malignant, drug- related and autoimmune causes, …

Heartland Virus Preclinical Research Project, Brian B. Gowen

Funded Research Records

No abstract provided.

Double-Port Totally Endoscopic Mitral Valve Replacement Through A Prosthesis-Sized Working Incision With Posterior Subvalvular Preservation, Cao Dang Khang, Tran Duc Quang, Phan Quang Thuan, Vuong Ngoc Minh, Pham Tran Viet Chuong, Nguyen Hoang Dinh

Journal of the Saudi Heart Association

Objective: To evaluate the feasibility and early outcomes of totally endoscopic non-robotic mitral valve replacement using a double-port DCT approach with posterior subvalvular preservation.

Methods: In this single-center retrospective observational study, 76 consecutive patients underwent totally endoscopic non-robotic mitral valve replacement between November 2023 and December 2025. The DCT approach was defined by exclusive endoscopic visualization, no rib spreading, a camera port and prosthesis-sized working incision placed in the same intercostal space, and thoracoscopic intracardiac instrumentation. Posterior ventricular-annular continuity was preserved whenever feasible by posterior leaflet preservation, native chordal preservation, or artificial chordal reimplantation after leaflet resection. Outcomes were reported …

Hemodynamic Characterization Of Cardiac Dysfunction After Traumatic Brain Injury Using A Controlled Cortical Impact Model In Male Rats, Preston So, Amit Iyengar, Noah Weingarten, Mrinal Patel, Jessica Dominic, Andrew Belec, Joyce Ho, Samuel Shin, Pavan Atluri

Division of Cardiology Faculty Papers

Traumatic brain injury (TBI) is associated with cardiovascular dysfunction that worsens clinical outcomes, yet the underlying mechanisms are poorly under stood. Clinical studies are limited by heterogeneity, highlighting the need for con trolled experimental models. Controlled cortical impact (CCI) provides precise control of injury severity, and invasive pressure- volume (PV) loop analysis allows detailed hemodynamic assessment. Adult male Wistar rats underwent sham op eration or mild or severe CCI- induced TBI. PV- loop assessment and transtho racic echocardiography were performed at 8 or 24 h after TBI or sham. Serum epinephrine (EPI) and norepinephrine (NE) were quantified by ELISA. At …

Cultivating Participation: Developing An Evidence-Based Farm-Based Sensory Integration Protocol For A Pediatric Occupational Therapy Clinic, Katelyn Childers

Occupational Therapy Doctoral Capstones

The purpose of this doctoral capstone project was to develop a comprehensive, evidence-informed farm-based sensory integration framework that translates current evidence into practical clinical resources to support therapist education, caregiver engagement, and consistent implementation of occupation centered intervention at Zion Therapy Services.

Cortical Plasticity Following Peripheral Nerve Injury In The Upper Extremity, Samah Gassass

WUSM Theses and Dissertations – All Programs

Peripheral nerve injury (PNI) in the upper extremity results in significant sensorimotor impairment. Nearly 40-50% of patients affected by PNI fail to restore normal hand and arm function and recovery remains variable. Recovery related cortical plasticity is recognized as an important factor, though its relationship to meaningful outcomes remains unclear. This dissertation examined how cortical activity patterns relate to functional recovery after PNI through a systematic review and three subsequent cross-sectional studies.

The systematic review of multimodal neuroimaging studies on cortical plasticity after upper extremity nerve transfer found that good recovery is generally linked to restoration of contralateral or typical …

Characterization Of Zdhhc Palmitoyltransferase Expression During Retinoic Acid-Induced Differentiation Of Sh-Sy5y Neuroblastoma Cells, Mohammad Abdallah Matari

Seton Hall University Dissertations and Theses (ETDs)

Protein S-Palmitoylation is a reversible post-translational modification that regulates protein trafficking, membrane localization, and neuronal function through the activity of the zinc finger Asp-His-His-Cys (zDHHC) family of palmitoyl acyltransferases. Although SH-SY5Y neuroblastoma cells are widely used as an in vitro model of neuronal differentiation, the expression profile of the complete zDHHC family during retinoic acid (RA)-induced differentiation has not been systematically characterized. The objective of this study was to characterize the expression of all 23 human zDHHC genes during RA induced differentiation of SH-SY5Y cells. SH-SY5Y cells were differentiated using 10 µM all-trans retinoic acid for up to seven days. …

For Weight Loss: Why Do Glp-1 Agonists Work, And How Can We Make Them Work Better?, Charlise Giang, Jieyi Meng, Mahmoud Ali Mohammad, Zhenqi Liu, Xinle Wu, Yi Zhu

Children’s Nutrition Research Center Staff Publications

Glucagon-like peptide 1 receptor agonists (GLP-1RAs) have revolutionized the management of not only type 2 diabetes but also obesity. However, even with rapid therapeutic advances in GLP-1RAs, questions regarding the fundamental principles of GLP-1-mediated weight loss and the enhanced efficacy of next-generation therapies remain. In this comprehensive review article, we first examine the milestones of GLP-1, from its initial discovery and biological characterization to its successful translation into clinical therapy. Furthermore, this review highlights the latest clinical trial data, specifically HbA1c reduction, weight-loss efficacy, and safety profiles, while exploring current pipelines of major pharmaceutical companies. Additionally, we delineate the mechanistic …

Investigation Of The Functional Dimensions Of Teats Up To Mid-Lactation In Bulgarian Murrah Buffaloes Established By Ultrasonography, Ivan Fasulkov, Pencho Penchev, Yordanka Ilieva, Manol Karadaev

The Thai Journal of Veterinary Medicine

The aim of this study was to monitor the changes in teat measurements from early to mid-lactation in the Bulgarian Murrah buffaloes. The experiment was carried out with twenty (10 primiparous and 10 pluriparous) lactating buffaloes. Five series of ultrasound measurements were performed, each consisting of two sessions for each animal corresponding to the phases of lactation: first lactation month (day 1 to day 30 postpartum) and mid- lactation (day 122 to day 152). The studied parameters were teat canal length (TCL), teat diameter (TD), teat wall thickness (TWT), and teat cistern diameter (TCD). The data were processed using the …

A Comparative Analysis Of Clinical Quality And Readability Of Neuropathic Pain Patient Information Leaflets: Türkiye Versus The United States, Gonul Sari, Argun Pire

Turkish Journal of Medical Sciences

Background/aim: Neuropathic pain management requires high patient adherence and complex dose-titration regimens, making the readability of patient information leaflets (PILs) a critical patient safety factor. This study aimed to compare the clinical content quality and linguistic readability of PILs for neuropathic pain medications between Türkiye (TİTCK) and the United States (DailyMed).

Materials and methods: A total of 11 pairs of innovator medications were analyzed. Clinical content was assessed using the 15-item Keystone Clinical Content Checklist (KCCC). Readability was assessed using the Ateşman index for Turkish texts and the Flesch Reading Ease Score (FRES) for English texts. Information quality and reliability …

Changes In T-Cell Subsets And Their Relationships With Disease Severity In Patients With Alopecia Areata, Hati̇ce Türk Daği, Hülya Özdemi̇r, Mehmet Akyürek, Fatma Tunçez Akyürek, Hasi̇be Artaç

Turkish Journal of Medical Sciences

Background/aim: Alopecia areata (AA) is a nonscarring hair loss disorder characterized by genetic susceptibility, environmental triggers, and T-cell–mediated autoimmunity. While various CD4+ and CD8+ T-cell subsets are implicated in its pathogenesis, the roles of follicular T-cell (Tfh), peripheral T-cell (Tph), and regulatory T-cell (Treg) subsets– particularly within the CD8+ population–remain incompletely defined. This study aimed to characterize circulating CD4+ and CD8+ T-cell subsets in Turkish patients with AA and to evaluate their associations with disease activity, duration, and severity.

Materials and methods: A total of 40 patients with AA and 40 healthy controls were enrolled. CD4+ and CD8+ T-cell subsets—including …

Histomorphological Changes In Human Septal Cartilage Stored In Different Preservation Solutions ​, Özlem Akkoca, Aki̇f Si̇nan Bi̇lgen, Harun Çoban, Fatma Aslan Yay, Zeynep Kaptan, Funda Balkiliç Akkaya, Rahmi̇ Kiliç, Sami̇ Engi̇n Muz

Turkish Journal of Medical Sciences

Background/aim: To assess the histopathological effects of three preservation solutions (70% ethanol, povidone-iodine, and ciprofloxacin) on human septal cartilage obtained during septoplasty and to compare their preservation characteristics for potential future graft applications. Optimal preservation of septal cartilage is clinically important because well-preserved grafts may reduce the need for secondary cartilage harvesting and the associated donor-site morbidity while facilitating reconstructive procedures during revision surgery.

Materials and methods: A total of 90 septal cartilage specimens obtained from 30 patients undergoing septoplasty were allocated to three preservation groups and stored in the designated preservation solutions at +4 °C. Samples were examined histologically …

Erratum To “Effects Of Colchicine On Sah-Induced Vascular Damage In An Experimental Subarachnoid Hemorrhage Model’’ [Turkish Journal Of Medical Sciences 56 (2) 2026 597-607] ​, Emi̇ne Demi̇r, Cumaali̇ Demi̇rtaş, Cansu Sönmez, Eray Meti̇n Güler, Sarper Kocaoğlu, Hakan Beyaztaş, Eyüp Çeti̇n

Turkish Journal of Medical Sciences

The original article can be accessed at https://doi.org/10.55730/1300-0144.6192.

Background/aim: Subarachnoid haemorrhage (SAH) is a severe cerebrovascular condition with high morbidity and mortality. Inflammation and oxidative stress are key factors in its pathophysiology, particularly in secondary vascular injury. This study aimed to evaluate the effects of colchicine on vascular integrity and biochemical markers following SAH in an experimental rat model.

Materials and methods: Eighteen female Sprague-Dawley rats were divided into three groups: Control (no SAH), SAH (induced without treatment), and SAH + Colchicine (1 mg/kg intraperitoneally at 1 h and 24 h). SAH was induced by autologous blood injection into …

L-Theanine Attenuates Alcl3-Induced Cognitive Dysfunction And Neurotoxicity In Rats: A Behavioral And Biochemical Study, Muhammet Oğuzhan Dönmez, Azi̇ze Şener, Şeyda Karaman Ersoy, Mahdi̇ Marzi̇, Göksel Şener

Turkish Journal of Medical Sciences

Background/aim: The effects of L-theanine (LTN), a natural compound found in tea leaves, on biochemical parameters and cognitive functions were investigated in an aluminum chloride (AlCl3)-induced Alzheimer’s disease-like experimental model.

Materials and methods: A total of 32 male Wistar rats were allocated into four groups: control, AlCl3-induced AD-like group, LTN-treated AD-like group, and donepezil-treated AD-like group. Donepezil was used as a reference drug. For the induction of AlCl3- induced neurotoxicity, AlCl3 was administered by gavage at a dose of 25 mg/kg/day during the 1st week and 150 mg/kg/day for the subsequent 3 weeks. LTN was administered orally at a dose …

Therapeutic Potential Of Nasal Kisspeptin-54 For Reducing Α-Synuclein Accumulation And Restoring Hippocampal Synaptic Plasticity In A 6-Hydroxydopamine-Induced Rat Model Of Parkinson’S Disease ​, Ayşegül Gemi̇ci̇ Si̇nen, Emre Savran, Okyanus Bora Güleç, Osman Si̇nen, Nari̇n Deri̇n

Turkish Journal of Medical Sciences

Background/aim: Parkinson’s disease (PD) is characterized by progressive degeneration of dopaminergic neurons and pathological aggregation of α-synuclein (α-syn), leading to significant motor and cognitive impairments. Kisspeptin-54 (KP-54), a neuropeptide primarily involved in reproductive regulation, has recently been implicated in neurorestorative processes, with emerging evidence suggesting beneficial effects on locomotor activity and cognitive function. However, its therapeutic potential in PD has not yet been fully investigated. This study aimed to evaluate the therapeutic efficacy of chronic nasal KP-54 administration in a 6-hydroxydopamine (6-OHDA) rat model of PD, focusing on motor and cognitive outcomes, hippocampal synaptic plasticity, and α-syn pathology.

Materials and …

Boric Acid Enhances The Antitumor Effects Of Paclitaxel By Modulating Apoptosis, Autophagy-Related Protein Expression, And Proinflammatory Cytokine Expression In Ovcar-3 Ovarian Cancer Cells ​, Nazmi̇ye Bi̇tgen, Gözde Özge Önder, Münevver Baran, Fatma Esen Karakuş, Meli̇ke Akbulut, Arzu Yay

Turkish Journal of Medical Sciences

Background/aim: Ovarian cancer is the eighth most prevalent malignancy among women globally and ranks third among gynecological cancers in terms of incidence. The limited efficacy of standard chemotherapy, primarily due to intrinsic drug resistance and high recurrence rates, highlights the urgent need for novel therapeutic strategies that are both effective and selectively cytotoxic.

Materials and methods: This study aimed to investigate the combined effects of boric acid (BA), a biologically active boron compound with emerging antitumor potential, and paclitaxel (PTX), a conventional chemotherapeutic agent, on cell viability, cell cycle progression, apoptosis, autophagy-related protein expression, and inflammatory signaling in the chemoresistant …

Readability Of Dermatology Patient Education Materials: A Comparative Analysis Of Turkish Dermatology Association Brochures And Texts Generated By Chatgpt And Gemini ​, Ahmet Kağan Özdemi̇r, Bengü Reyhan Botsali Özdemi̇r, Akin Aktaş

Turkish Journal of Medical Sciences

Background/aim: In modern healthcare, patient education materials (PEMs) are essential for bridging the gap between clinical knowledge and patient understanding. However, in Türkiye, where 64.6% of the adult population possesses inadequate or problematic health literacy, traditional medical brochures often exceed recommended reading levels. This study aimed to evaluate and compare the readability of Turkish dermatology PEMs prepared by the Turkish Dermatology Association (TDA) with those generated by artificial intelligence (AI) models.

Materials and methods: A total of 68 TDA PEMs were analyzed. Readability was assessed using the Ateşman Readability Index. Responses were generated by AI models to questions extracted from …

Can Hydrolyzed Silk Fibroin Derived From Bombyx Hatayensis And Ozonized Gel Modulate Inflammatory And Angiogenic Responses In A Corneal Alkali Burn Model? ​​, Şule Barman Kaki̇l, Hamdullah Suphi̇ Bayraktar, Okan Soylu, İshak Gökçek, Meryem İlkay Karagül, Zehra Demi̇ray Asoğlu

Turkish Journal of Medical Sciences

Background/aim: The therapeutic effects of hydrolyzed silk fibroin derived from Bombyx hatayensis and a commercially available ozonized gel, administered individually and in combination, were evaluated in a rat model of corneal alkali burn.

Materials and methods: A standardized corneal alkali burn was induced in the right eyes of Wistar albino rats using 1 N sodium hydroxide (NaOH). Animals were randomly assigned to six groups: negative control, positive control (PC), hydrolyzed silk fibroin (SF), ozonized gel (O), combined treatment (SF + O), and antibiotic treatment. Topical treatments were administered for 21 consecutive days. Clinical evaluation included assessments of corneal opacity, neovascularization, …

Antiobesity, Antidiabetic, And Antioxidant Activities Of Selected Rubia Species (Rubiaceae) And Lc–Ms/Ms-Based Chemical Profiling ​, Semi̇h Bulut, Ahmet Kahraman, Ayşenur Işik, Mustafa Abdullah Yilmaz

Turkish Journal of Medical Sciences

Background/aim: Rubia species (Rubiaceae) are recognized as medicinal and industrial plants exhibiting diverse biological activities. This study was conducted to evaluate the antioxidant, antidiabetic, and antiobesity potential of extracts prepared from Rubia davisiana Ehrend. (an endemic species), R. peregrina L., and R. rotundifolia Banks & Sol., which grow naturally in Türkiye.

Materials and methods: The antioxidant activity of 80% ethanol extracts prepared from the aerial parts was evaluated using 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assays, ferric reducing power, metal chelating capacity, and total antioxidant capacity assays. Inhibition assays for α-glucosidase and α-amylase were performed to evaluate antidiabetic activity, …

Face Mask Littering: Environmental Impacts, Regulatory Challenges, And Ai-Driven Waste Management Strategies For The Post-Pandemic Era, Pri̇yanka Rathee, Sari̇ta Khatkar, Renu Sehrawat, Pooja Rathee, Anurag Khatkar, Esra Küpeli̇

Turkish Journal of Medical Sciences

The widespread use of polypropylene-based face masks during the COVID-19 pandemic created a significant and persistent source of plastic pollution. This review aims to provide an integrative assessment of the environmental, toxicological, and public health implications of face mask litter and evaluate current waste management challenges and emerging technological solutions in the postpandemic context. A narrative review approach was employed, synthesizing literature published between 2020 and 2025 and indexed in major scientific databases including Web of Science, Scopus, and PubMed. Studies were selected based on their relevance to the issues of environmental contamination, microplastic generation, toxicological effects, regulatory frameworks, and …

Trace Elements In Complete Feeds Used In Intensive Rearing Of Cattle, Poultry, And Pigs: Occurrence And Predicted Transferred Concentrations In Animal-Derived Food, Elizabeta Dimitrieska Stojkovikj, Vangelica Enimiteva, Aleksandra Angjeleska, Risto Uzunov

Turkish Journal of Veterinary & Animal Sciences

This study investigated the concentrations of essential (Zn, Cu, Mn, Co, and Mo) and toxic (Pb, Cd, As, and Hg) trace elements in complete feeds used for the intensive rearing of cattle, poultry, and pigs in North Macedonia and predicted their transfer into animal-derived foods. A total of 107 feed samples were collected from 40 farms between May 2023 and April 2024 and analyzed using inductively coupled plasma mass spectrometry. The results revealed that while most essential elements were within the regulatory limits, Co exceeded the maximum level in two cattle feed samples. Zn, Cu, and Mn levels were generally …

Molecular Characterization Of Pasteurella Multocida Capsular Type A Strains Isolated From Different Regions Of Anatolia, Türkiye​, Adam Bashir Tawor, Osman Ergani̇ş, Asli Balevi̇, Suliman Mohamed Yousof Sadam

Turkish Journal of Veterinary & Animal Sciences

Pasteurella multocida serotype A is an important pathogen that causes respiratory illness in both farm animals and poultry, leading to severe economic losses worldwide. This study aimed to isolate P. multocida capsular type A (capA) as well as investigate the virulence-associated genes (VAGs) within the isolates. Samples (n = 250) were collected in the Anatolia region from cattle (n = 130), sheep (n = 70), goats (n = 45), and pet birds (n = 5) with clinical respiratory signs. Polymerase chain reaction (PCR) assays were conducted using kmt1 and capA primers for P. multocida species identification and capsular serogroup A …

Effect Of Rangeland Quality On Rumen Development Of Angora (Mohair) Goat Kids And Bucks, Betül Zehra Sariçi̇çek

Turkish Journal of Veterinary & Animal Sciences

This study was conducted to determine the effect of rangeland quality on the rumen development of Angora goat kids and bucks. Twenty male singleton Angora goat kids with similar birth dates and birth weights were selected from each farm in an area with three different rangeland quality levels (good, medium, or poor) in Ankara Province. The kids were weaned at 60 days of age and then grazed completely on rangeland until slaughter. Rumen volumetric and papillary development of animals slaughtered at 4 and 7 months of age were determined. Empty rumen length, width, and weight were affected by rangeland quality …

Hard Palate Morphology In The Anatolian Water Buffalo (Bubalus Bubalis), Mehmet Can, Şükrü Hakan Atalgin, Mehmet Faruk Aydin, Soner Bekmezci̇

Turkish Journal of Veterinary & Animal Sciences

This study aimed to examine the morphological and histological features of the hard palate in the Anatolian water buffalo through light and scanning electron microscopy, complemented by gross and morphometric evaluations. Eight hard palates were used in the study. A total of eight Anatolian water buffalo heads from healthy adult female water buffaloes were collected immediately after slaughter from various slaughterhouses in Balıkesir, Türkiye. After analyzing and identifying the obtained hard palate samples, measurements were taken and documented through photography. The papilla incisiva, located in the middle of the caudal concave edge on the ventral surface of the pulvinus dentalis, …

Arterial Architecture Of The Goat Spleen (Capra Hircus) Revealed By Corrosion Casting And Scanning Electron Microscopy, Baran Erdem, Sevi̇nç Ateş, Meryem Karan

Turkish Journal of Veterinary & Animal Sciences

The spleen is an essential organ involved in hematopoietic and immune functions. A comprehensive understanding of its anatomy, including vascular topography and segmentation, is essential for the accurate diagnosis and treatment of splenic diseases, and the minimization of complications during surgical procedures. Detailed knowledge of the vascular architecture also supports the accurate prediction and management of hemorrhage severity in cases of splenic rupture. The spleen’s microvascular system has a unique and complex organization that is distinct from other organs, and its elucidation is critical for understanding splenic function. In the present study, the spleens of 20 hair goats were examined. …

Full Issue, Volume 14, Number 1., Donna J. Peterson Dr., Scott Cummings, Elizabeth Gregory North

Journal of Human Sciences and Extension

No abstract provided.

Radiographic Evaluation Of Kidney Size In Cats: Effects Of Sex And Gonadal Status, Somchin Sutthigran, Auraiwan Klaengkaew, Chutimon Thanaboonnipat, Suppawiwat Ponglowhapan, Nan Choisunirachon

The Thai Journal of Veterinary Medicine

Radiographic evaluation of kidney size using the kidney-to-second lumbar vertebral body length (K/L2) ratio is a standard method for assessing kidney size in cats. However, current reference values underdetermine biological factors such as sex and gonadal status, which may influence kidney and lumbar length. Reproductive hormones are known to influence kidney and vertebral development, but their specific effects on radiographic kidney and vertebral measurements in cats are unclear. This retrospective study aimed to assess the effects of gonadal status on kidney length, L2 vertebral length, and the K/L2 ratio in clinically healthy cats. A total of 285 cats were included …

Telomere-To-Telomere Genome Assembly And A Pangenome For The Rat, Kai Li, Julia L Ciosek, Sergey Koren, Adam M Phillippy, Yaming Zhu, William A Lauer, Shelise Y Brooks, Gerard G Bouffard, Brandon D Pickett, Beth L Dumont, Melissa L Smith, Theodore S Kalbfleisch, Peter A Doris

The Brown Foundation: Institute of Molecular Medicine

We report a complete rodent telomere-to-telomere genome assembly from the brown rat, Rattus norvegicus. Annotation was enriched with multi-tissue long-read RNA sequencing and uncovered numerous novel genes. Assembly of both sex chromosomes reveals the absence of gene coding in the presumed pseudo-autosomal regions and the presence of centromeric satellite repeats on distal chromosome Y (chrY). We provide evidence of meiotic conjunction between Xp and Yq. The genome assembly reveals several expanded autosomal regions enriched for testis-expressed genes. Finally, we have generated a pangenome from recent high-quality assemblies of 8 distinct inbred rat strain genomes. This allows the strain-specific distribution of …

Pre-Implementation Factors For Active Music Engagement (Ame): Barriers And Facilitators To Integrating An Evidence-Based Dyadic Intervention Into Pediatric Oncology Care., Kristin M. Story, Elizabeth Harman, Kristin Stegenga, Jelena Golubovic, Sheri L. Robb

Manuscripts, Articles, Book Chapters and Other Papers

BACKGROUND: Pediatric cancer treatment is a difficult and distressing experience that often results in sustained, interrelated distress in young children and parents, and traumatic stress symptoms for some parents. Few evidence-based interventions address this dyadic distress. Active Music Engagement (AME) is a manualized, evidence-based music play intervention that significantly reduces distress in child/parent dyads and mitigates traumatic stress and improves well-being among highly distressed parents and those experiencing sociodemographic risk. Successfully integrating AME into standard care requires a systematic understanding of implementation factors. This study aimed to identify site-specific barriers and facilitators that influenced successful AME delivery during a recently …

Bcg Overexpressing Ag85b Reprograms Host Immune Response Through Coordinated Transcriptional Networks, Vivek Chauhan, Rakesh Kumar, Raja Veerapandian, Enrique I. Ramos, Chinnaswamy Jagannath, Shrikanth Gadad, Subramanian Dhandayuthapani

School of Medicine Publications

Antigen 85B (Ag85B), an immunodominant protein secreted by Mycobacterium tuberculosis (Mtb), induces robust T-cell-mediated immunity in host cells. Previous studies have demonstrated that recombinant BCG strains overexpressing Ag85B (BCG85B) confer significant protection against tuberculosis in mouse models. This study hypothesized that enhanced protection by recombinant BCG85B results from modulation of host immune gene expression by the Ag85B antigen. To test this, RNA-seq analysis was performed on splenocytes from mice immunized with the wild-type BCG (BCG-WT) and BCG85B strains. Differentially expressed genes (DEGs) in the splenocytes of BCG85B-immunized mice indicated upregulation of immune-related KEGG pathways, including Th1/Th2 and Th17 differentiation, NF-kappa …