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The Duration Of The Effect Of Different Carbohydrates On Neutrophilic Phagocytosis, Judy L. Reeser

Loma Linda University Electronic Theses, Dissertations & Projects

In order to determine the duration of the effect of glucose, fructose, sucrose, honey, starch, and orange juice on neutrophilic phagocytosis, 10 subjects underwent a complete series of carbohydrate tolerance tests. Blood samples were drawn at specific time periods following ingestion of 100 grams of each carbohydrate. Blood was then analyzed for glucose and phagocytic index (the mean number of bacteria engulfed per leukocyte); a red blood cell and white blood cell count and differential white cell count were done also on about 1/2 of the samples. In addition, 7 other subjects participated in a fasting study in which no …

Cardioregulatory Role Of The Abdominal Ganglion Of Aplysia Californica, George Maeda

Loma Linda University Electronic Theses, Dissertations & Projects

An isolated heart-abdominal ganglion preparation of this mollusc was developed and used to study the cardioregulatory function of the ganglion and nerves. The heart was perfused with artificial sea water through an atrial cannula and the gastro-esophageal artery was catheterized for blood pressure monitoring. The presence and cardioregulatory effects of axons in the nerves was investigated by electrical stimulation. Fine-tipped glass microelectrodes inserted into ganglionic neurons were used to determine the relationship between the neuronal and cardiac activities.

The principal results are listed below.

1. Primarily inhibitory responses are obtained from both right and left connective stimulation, with some evidence for …

An Anesthetic Technique Utilizing Innovar, Fentanyl, And Methohexital Sodium For Outpatient Oral Surgery, Fredric D. Haerich

Loma Linda University Electronic Theses, Dissertations & Projects

A study of 800 patients ranging in age from seven to one hundred years was conducted in the development of an anesthetic technique which combines the desirable qualities of a neuroleptic agent with the requirements of an outpatient oral surgical anesthetic. These requirements included rapidity of onset, effectiveness of action, ease of controlling duration, and rapid postoperative recovery.

Patients who were cleared medically for outpatient procedures were given Innovar 1.5 cc/150 lb of body weight, atropine 0.4 mg, and fentanyl 1.0 cc/150 lb of body weight as an initial dose within 30-45 seconds in a D5W drip by means of …

Diffuse Head Cooling As A Method For Producing A Gradient Of Brain Hypothermia And Decreased Blood Volume, Arthur Cushman, Niall Horn, Stanley Rouhe, George Austin, Conrad Ciesel

The Dr. George M. Austin Neurology Research Collection

Diffuse Head Cooling as a Method for Producing a Gradient of Brain Hypothermia and Decreased Blood Volume. Arthur Cushman, M.F., Niall Horn, Ph.D., Stanley Rouhe, M.D., George Austin, M.D., Conrad Ciesel. Paper submission for the ACS Meeting, Atlantic City, New Jersey (October 21, 1971). Typewritten manuscript, 3 pg.

Faculty Publications July 1969 - June 1970, Lsu Health Sciences Center New Orleans

Historical Faculty Publications

This Faculty bibliography contains 164 publications.

The Parenchymal Cells Of The Rat Pineal Gland, E Tapp, M Blumfield

Faculty, Staff and Students Publications

Background: Immune-mediated thrombotic thrombocytopenic purpura (iTTP) is a rare hematologic disorder with improved survival due to advancements in treatment. However, long-term cardiovascular morbidity and mortality remain significant. Established cardiovascular risk calculators, such as the 2008 Framingham Heart Study (FHS) global cardiovascular disease (CVD) and the American College of Cardiology/American Heart Association (ACC/AHA) atherosclerotic CVD (ASCVD) risk estimators, may not adequately account for the elevated and unique cardiovascular risks in iTTP survivors.

Objectives: To evaluate the discrimination and calibration of the ACC/AHA ASCVD and FHS global CVD models in predicting major adverse cardiovascular events (MACEs) among iTTP survivors.

Methods: This retrospective …

The Development Of Electrophysiological Techniques Allowing "In Situ" Measurement Of Electrical Changes Within Smooth Muscle Cells Of Mesenteric Micro-Vessels, William J. Willems

Master's Essays (1922 - )

The primary objective or this study is essentially to acquire further knowledge of the factors involved in the nervous and humoral control of vascular smooth muscle tone. The relative importance of the nervous and endogenous vasotropic humoral control or vasomotor tone under normal conditions as well as under conditions or hypotension can be evaluated, at least in part, by observing the electrical activity occurring in single vascular smooth muscle cells of the mesenteric microcirculation. This thesis describes: 1) the development of electrophysiological methods for the measurement of such activity in ''in situ" micro-vessels with circulation and innervation intact, and 2) …

A Study To Demonstrate The Immediate Destination Of An Embolus Formed In The Lumen Of A Peripheral Venous Catheter, Rosemarie Morikone Emori

Loma Linda University Electronic Theses, Dissertations & Projects

This study was designed to show the immediate destination of an embolus formed in the lumen of an intravenous catheter placed in a peripheral vessel. The hypothesis stated that clots injected into a peripheral vessel will lodge in the lungs. A subhypothesis was that the clot may lodge anywhere along the circulatory pathway.

Indications of need for the study as shown by the review of literature were the following: (1) the complications resulting from the frequent use of intravenous polyethylene catheters, (2) experimental studies that showed that clots were formed around intravenous catheters, (3) the evidence that intravenous catheters predispose …

A Comparative In Vitro Metabolic Study Of Certain Pesticidal Phenyl N-Methyl-Carbamates, Larry A. Wheeler

Loma Linda University Electronic Theses, Dissertations & Projects

Two critical parameters in the evaluation of the toxicological hazards of the use of carbamate pesticides are: (1) a rapid, sensitive, and precise analytical method of identification of the carbamate and its metabolites; and (2) their metabolism. A method in this study was developed whereby various carbamates and their metabolites could be chromatographed in the gaseous phase. Also, a comparative in vitro study of 15,000 g liver supernatants from human, rat, and dog was performed. Additional tissues investigated for metabolic activity toward the carbamates, Zectran and Mesurol, were kidney (from the dog only) and various blood fractions (red blood cells, …

The Purification, Properties And Subunit Structure Of Glycerol Dehydrogenase, Michael James Barrett

Theses and Dissertations (ETD)

The inducible, NAD-linked glycerol dehydrogenase (E.C. 1.1.1.6) of a guanine requiring mutant of A. aerogenes has been purified to homogeneity. The molecular weight of the pure enzyme was found to be 3.4 x 10⁵ daltons. The sedimentation coefficient of the enzyme was 10.7 x 10^-13 sec.^-1. The diffusion constant was 3.07 x 10^-7 cm²/sec.

The partial specific volume calculated from the amino acid composition was 0.72 ml/g. The following kinetic parameters were determined; K_m glycerol, 2.4 x 10^-3 m, K_m NAD, 2.8 x 10^-4 M, K_m dihydroxyacetone, 5.1 …

The Effect Of Exercise On Metabolic Rate With Time In Overweight And Normal Weight Subjects, Roberta Jewell Keswick

Loma Linda University Electronic Theses, Dissertations & Projects

Previous studies in the Lome Linda University laboratory showed that obese subjects on an exercise program lost approximately one pound more per week as compared to a non-exercise group. Energy expenditure calculations of the exercise did not account for the total caloric deficit required to lose the additional pound. The present study was designed to investigate changes in BMR and metabolic rate with time for normal weight and overweight subjects involved in a practical exercise program. The relationship of changes in BMR and metabolic rate in coping with the problems of obesity were also studied.

Fifteen subjects, eight overweight and …

Dietary Factors And Induction Of Hepatic Microsomal Hydroxylative Enzymes By Organochlorine Pesticides, D. Jesse Wagstaff

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Induction of hepatic microsomal hydroxylative enzymes is an important aspect of detoxication of fat-soluble toxicants. The magnitude of induction depends on numerous factors, such as the nature and dose of toxicant as well as dietary factors. Research was conducted on (1) endrin tolerance in rats, (2) preliminary comparisons of inductive effects of various organochlorine pesticides in rats to select compounds for further study in guinea pigs, (3) general effects of various dietary factors on induction, and (4) effects of ascorbic acid deficiency on induction of hepatic microsomal hydroxylative enzymes by organochlorine pesticides in guinea pigs. Measurements were made of body …

Some Aspects Of Carbohydrate Metabolism In The Cercariae Of Schistosoma Mansoni Sambon, 1907, A. Eugene Dunham Jr.

Loma Linda University Electronic Theses, Dissertations & Projects

Cercariac of Schistosoma mansoni were studied for glycogen utilization, uptake of glucose from solution, lactic acid production, and respiration.

Using the anthrone method of determination of carbohydrate, the amount of glycogen used by a single cercaria in 4 hours is determined as 43.2 x 10^-4 μg ± 6.6 x 10^-4 μg.

Glucose was not observed to have been used from solution under the conditions of the experiment, even in the presence of trehalose. The determinations were made by the Glucostat method.

After deproteinization and desugarization, aliquots of supernatant from cercarial suspensions were analyzed for lactic acid using p-phenylphenol …

Pneumonia Due To Mycoplasma In Gnotobiotic Mice: Ii. Localization Of Mycoplasma Pulmonis In The Lungs Of Infected Gnotobiotic Mice By Electron Microscopy, Avrum B. Organick, Kenneth A. Siegesmund, Irving I. Lutsky

Biomedical Sciences Faculty Research and Publications

Organick, Avrum B. (Marquette University School of Medicine, Milwaukee, Wis.), Kenneth A. Siegesmund, and Irving I. Lutsky. Pneumonia due to mycoplasma in gnotobiotic mice. II. Localization of Mycoplasma pulmonis in the lungs of infected gnotobiotic mice by electron microscopy. J. Bacteriol. 92:1164–1176. 1966.—Lesions in lungs of gnotobiotic mice inoculated intranasally with Mycoplasma pulmonis were examined by electron microscopy after osmic acid fixation. At 1 week after infection, mycoplasma cells were found in large numbers in the bronchi at the surface of bronchial epithelial cells and, in smaller numbers, in the alveoli where active phagocytosis by polymorphonuclear leukocytes (PMN) occurred. …

The Improvement Of Peripheral Blood Flow During Hemorrhagic Hypotension Following Phenoxybenzamine Treatment Expressed In Terms Of Extracellular Potassium Shifts, Thomas J. Wera

Master's Essays (1922 - )

The study of plasma electrolytes notably, the potassium and sodium ions during hemorrhagic stress and other forms of shock, were once the subject of intense investigation (42). However, the advent of new investigative techniques enabled more complex, experimental designs and methods to be used in which it was possible to study the many other variables which exist as a result of shock regardless of cause. The proponents of the probable electrolyte mechanism in shock subsequently diminished in numbers until recent times.

It is felt by this author that recent renewal regarding the various ions, especially the cations, sodium and potassium …

Responses Of The Normal Human To Infusions Of Plasma From Patients With Graves' Disease, Claude D. Arnaud, Hans A. Kneubuhler, Virginia L. Seiling, Bruce K. Wightman, Norman H. Engbring

Biomedical Sciences Faculty Research and Publications

No abstract provided.

The Effect Of Varying Protein Intake On Trichinella Spiralis Infection In Mice, Clare K. Kwan

Loma Linda University Electronic Theses, Dissertations & Projects

Three hundred Swiss Webster albino mice three weeks of age were placed on low (8%), medium (24%), high (48%) protein levels and a commercial laboratory animal feed, Purina Chow for various lengths of time: (1) simultaneously with infection, and four weeks thereafter, (2) three weeks before infection and throughout the four-week infection period, and (3) six weeks prior to infection and during the infection period. The protein source was casein. Sixty of the 300 mice used served as noninfected controls, and the other 240 were each infected with 400 washed, suspended Trichinella spiralis larvae obtained from a rat by the …

Viral Infections Of Domestic Animals, George A. Young

School of Veterinary and Biomedical Sciences: Faculty Publications

There is a strong tendency in current research on mammalian virus diseases to concentrate on virus activity at a cellular or subcellular level. This trend is summarized adequately by Enders in relation to viral replication as follows: ','The large and ever-increasing volume of published experimental work on viral replication strikingly reveals the central position of this phenomenon in contemporary virological research. There are good reasons, both biological and practical, underlying this intensive effort to understand, i n detail, how a virus particle, without energy transforming apparatus of its own, manages to utilize the metabolic equipment of the cell to produce …

Faculty Publications July 1963 - June 1964, Lsu Health Sciences Center New Orleans

Historical Faculty Publications

This Faculty bibliography contains 156 publications.

The Ultrastructure Of The Intranuclear Rodlet In Certain Nerve Cells, Kenneth A. Siegesmund, C. R. Dutta, C. A. Fox

Biomedical Sciences Faculty Research and Publications

No abstract provided.

The Conversion Of Cytidylic Acid To Deoxycytidylic Acid In Rat Kidney, Judith Marie Werderitsch

Master's Essays (1922 - )

Evidence was presented in 1949 by Bendich, Getler, and Brown (2), that the free pyrimidine base, cytosine, was not incorporated into the nucleotides of deoxyribonucleic acid (DNA). Further studies by Hammarsten, Reichard, and Saluste (9) with cytidine and those by Roll, Weinfeld, and Carroll (26) with cytidylic acid, showed that the nucleoside and nucleotide derivatives were well incorporated into DNA. These experiments indicated that the conversion of the riboside (or ribotide) to the deoxyribose form had occurred with the nucleosidic linkage intact. This theory was further substantiated by Rose and Schweigert (29) using cytidylic acid incorporation into the total viscera …

Worm Eggs: Cost You Money, E.F. Frolik, E.W. Janike

School of Veterinary and Biomedical Sciences: Faculty Publications

Contents

One Million Eggs Daily.......... 3

Damaging Trip......... 4

Worm Remedies......... 5

Modern Worm Remedies......... 6

A Control Program.......... 8

Concerning The Innervation Of The Upper Dentition In Man, Jess Hayden Jr.

Loma Linda University Electronic Theses, Dissertations & Projects

The primary objectives of this study were to determine: 1) the pathway of the superior alveolar nerves and their relation to the maxillary molar tooth buds in the fetal and neonatal specimens and in the child, and 2) whether nerve fibers entered any microscopically-observed tooth buds.

A Study Of Regeneration Of Peripheral Nerve In The Rat, James C. Jay

Loma Linda University Electronic Theses, Dissertations & Projects

Due to improvements in fixation and embedding techniques a restudy of regenerating peripheral nerve was thought advisable in order to better understand and describe the processes which take place.

Femoral nerves of white Wistar rats were used. The lesions were made by either crushing or severing the nerve. After a period of regeneration, varying from 12 hours to six days, the wound was reopened and the injured portion fixed in situ with 1% osmium tetroxide. The tissues were then removed, dehydrated, embedded and sectioned. The sections were stained and studied with a modified RCA EMU2A electron microscope.

Observations revealed that …

Effect Of Isocaloric Unsaturated Fats On Serum Cholesterol Of Patients Treated With Hypocholesterolemic Agents, Yvonne Marie Hatley

Loma Linda University Electronic Theses, Dissertations & Projects

The effect of a high unsaturated-low saturated fat diet on serum cholesterol levels were studied. Subjects were clinic patients receiving the following hypocholesterolmeic agents: triparanol (MER-29), diildothyroacetic acid (Diac), dextrothyroxine (d-T₄) and 3-methoxy-16, alpha-methyl-1,3,5, (10)-estratriene-16 beta, 17 beta-diol (Manvene or Anvene).

Fifteen hypercholesterolemic patients, eleven of whom were diabetic, were placed on diets with prescribed amounts of unsaturated fat. The caloric distribution was 40 per cent carbohydrate, 20 per cent protein, and 40 per cent fat. The level of unsaturated fat was 70 per cent of the total fat.

Seventy-one per cent of the patients on the hypocholesterolemic …

Effect Of Between-Meal Eating On Stomach Emptying Time, Bernice Peterson

Loma Linda University Electronic Theses, Dissertations & Projects

As yet there is little agreement among health workers as to whether the practice of between-meal eating is harmful, beneficial, or indifferent in its influence on health. Studies which have been done heretofore on between-meal eating have been concerned with the effects on growth, appetite, daily nutrient intake, regularity, body metabolism, overweight, work efficiency, and dental caries. It appears that the possible physiological effects of between-meal eating have been only partially explored. The problem of this study was the effect of eating between meals on stomach emptying time.

The effect of between-meal eating on the emptying time of the stomach …

Studies Of Cardiovascular Energy Changes Occurring With Drug Administration Under Normal Acid Base Balance And During Hypercapnia, Ischemia, And Metabolic Acidosis, Earl E. Aldinger

MUSC Theses and Dissertations

Reflex autonomic changes occurring with the use of cardiovascular drugs may, as a side effect, increase cardiac work and oxygen demand. Improvement of coronary flow and/or a decrease in myocardial work load without increasing oxygen utilization are the desired effects of drugs used in the treatment of coronary insufficiency. The improvement of cardiac function is the purpose of drugs used in the treatment of heart failure. In either case the therapeutic effect may be accomplished through a direct myocardial effect and/or a decrease in cardiac pressure work load through extracardiac action. Through animal experimentation and patient correlation of the many …

The Assay And Properties Of Labile Factor (Factor V), Armand J. Quick

Biomedical Sciences Faculty Research and Publications

Human oxalated plasma stored at 4° C. until the prothrombin time is increased beyond 60 sec. is a reliable medium for assaying labile factor (factor V) because its response to added labile factor corresponds quantitatively to that of plasma from patients with congenital deficiency of this factor. Such an agreement is not obtained with plasma stored at 37°C. The stability of labile factor is closely associated with ionized calcium. The addition of thrombin to fresh oxalated plasma causes an apparent hyperactivity of labile factor, but this is completely removed by adsorption with Ca₃(PO)₂. Oxalated plasma when …

Physiologic Circadian Systems, Franz Halberg, Ruth Loewenson, Ralph Winter, Jacob Bearman, Galen H. Adkins

Journal of the Minnesota Academy of Science

No abstract provided.

The Effects Of Reserpine On Adrenal Responses To Nicotine And On Cardiovascular Responses To Exogenous Epinephrine And Norepinephrine, Ervin E. Bagwell

MUSC Theses and Dissertations

Since the isolation or reserpine by Bein et al (1) in 1952, there have been numerous investigations dealing with the pharmacological and physiological properties of this drug. A good proportion of this work has dealt with the effects of reserpine on the function of the sympatho-adrenal system. The present investigation has two primary objectives and is therefore divided into two chapters. The first chapter is concerned with a study of the effects of previous reserpine treatment on the responsiveness of the adrenal medulla to chemical stimulation and the reactivity of the cardiovascular system to this stimulation. In chapter two an …