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Different Hypoxic Response Of Human Cavii And Caenorhabditis Elegans Homologous Genes Cah-3, Cah-4, Cah-5, Sümeyye Aydoğan Türkoğlu, Aysu Bozkurt, Fatma Poyrazli, Derya Okuyan

Turkish Journal of Biology

Background/aim: A number of carbonic anhydrase (CA) family proteins have been implicated in cancer. They contribute to the hypoxic microenvironment. CAVII is often downregulated in colorectal carcinoma and it has been associated with increased tumor size, node metastasis, and adverse clinical outcomes. In this study, we aimed to investigate the effect of hypoxia on CAVII protein in human colon cancer and prostate cancer cells. In addition, the regulation of CAH genes in Caenorhabditis elegans was examined. These are homologous to CAVII in humans.

Materials and methods: CAVII expression was analyzed in different cell lines such as human colon cancer (SW480 …

Recreational Boat Fishing Participation Varies Across Bioregional Fisheries In Western Australia, With Persistent Patterns Within Fisheries And Fisher Typologies Over Time, Karina L. Ryan, Eva Lai, Claire B. Smallwood, Jeremy M. Lyle, Kate E. Stark, Sean R. Tracey

Fisheries Research Articles

Recreational fishing is popular worldwide and understanding variability in participation can inform policies that seek to improve sustain[1]ability of recreational fisheries and amenity for recreational fishers. However, not all recreational fishers are alike in their demographics or behaviours. This study explores temporal and spatial variability in licensed boat-based recreational fishing participation in Western Australia using data from a consistent survey design delivered 12 times from 2011 to 2024. Five fisher participation typologies were identified across four marine bioregions (North Coast, Gascoyne Coast, West Coast, and South Coast) and four fishery types (demersal, nearshore, pelagic, and invertebrate) with varied fisher characteristics …

Challenges In Modelling The Impact Of Frost And Heat Stress On The Yield Of Cool-Season Annual Grain Crops, Jonathan Richetti, Victor Oscar Sadra, Di He, Brenton Leske, Pengcheng Hu, Yacob Beletse, C. Mariano Cossani, Ha Nguyen, Bangyou Zheng, David Matthews Deery, M. Fernanda Dreccer, Jeremy Whish, Julianne Lilley

Grain and Other Field Crops Research Articles

Frost and heat events at critical growth stages could cause large yield losses. These temperature extremes are increasing in frequency and intensity due to climate change in many parts of the broadacre cropping regions globally, presenting challenges to food production. For cool-season grain-growing regions, where summers are already too hot, heat and frost risks can limit adaptation options. Capturing these stresses in crop models accurately is increasingly important for evaluating the timing, severity, and yield consequences of extreme events. However, most existing process-based models were not designed to simulate short-duration temperature extremes, limiting their ability to assess climate risk and …

Efficacy Of Lactobacillus Rhamnosus And Its Metabolites To Mitigate The Risk Of Foodborne Pathogens In Hydroponic Nutrient Solution, Esther Oginni, Robin A. Choudhury, Veerachandra Yemmireddy

School of Earth, Environmental, & Marine Sciences Faculty Publications

Hydroponic nutrient solution (HNS) has been established as an ideal conduit for pathogen contamination and proliferation. This study evaluated the efficacy of lactic acid bacteria and their metabolites in mitigating the risk of foodborne pathogens in HNS when compared to conventional chemical treatments. Hoagland’s HNS were prepared according to the manufacturer’s instructions and inoculated with Salmonella Typhimurium, Escherichia coli 0157:H7, and Listeria innocua at 105 CFU/mL cell concentration. These nutrient solutions were subjected to treatment with various concentrations of Lactobacillus rhamnosus live cells, a cell-free extract (CFE) of L. rhamnosus metabolites, sodium hypochlorite and peroxyacetic acid at 22 ± 1 …

Homologous And Heterologous Vaccination Regimens With Mrna And Rvsv Platforms Induce Potent Immune Responses Against Sftsv Glycoprotein, Tomaz B. Manzoni, Jonna B. Westover, Kendall A. Lundgreen, Philip D. Hicks, Raegan J. Petch, Jordan T. Ort, Drew Weissman, Steven H. Y. Fan, Scott E. Hensley, Norbert Pardi, Brian B. Gowen, Paul Bates

Animal, Dairy, and Veterinary Science Faculty Publications

Background: Severe fever with thrombocytopenia syndrome virus (SFTSV) is a highly pathogenic bunyavirus with a high case-fatality ratio for which there is no approved vaccine. Studies have assessed different vaccine technologies. However, few studies have yet assessed the immunogenicity of heterologous prime-boost regimens. Methods: Here, we compare a lipid nanoparticle (LNP)-encapsulated nucleoside-modified mRNA-based vaccine encoding the SFTSV glycoproteins, Gn and Gc, to our recently described recombinant VSV SFTSV (rVSV-SFTSV) vaccine in single dose, homologous, and heterologous prime-boost regimens in mice. Results: We show that all regimens protect from pathogenic SFTSV challenge and elicit strong long-lasting antibody responses. Furthermore, strong cellular …

Effect Of Histidine Tag On The Enzymatic Activity Of Pseudolysin And Its Interaction With The Streptomyces Metalloprotease Inhibitor (Smpi) Protein, Adewale Adeboye Adewole

Electronic Theses and Dissertations

Pseudolysin (PLN) protein is a major virulent factor in Pseudomonas aeruginosa infection. PLN belongs to the family of zinc-dependent metalloproteases. It is naturally inhibited by a smaller protein called streptomyces metalloprotease inhibitor (SMPI), secreted by Streptomyces nigrescens. Previous and current studies demonstrate that the interaction between PLN and SMPI offers unique mechanistic insights for drug development against infections caused by P. aeruginosa. This study investigated the effect of N-terminal Histidine (His) tag on the enzymatic activity of PLN and its interaction with SMPI. His-tagged PLN and SMPI proteins were expressed in bacteria and purified by affinity chromatography …

Post-Drought Growth Recovery Of Tea (Camellia Sinensis) Under Different Techniques And Doses Of Biofertilizer Applications, Nabila Ragil Wiharti, Intan Ratna Dewi Anjarsari, Yudithia Maxiselly

Jurnal Kultivasi

Drought stress is a major limiting factor affecting the growth, yield, and quality of tea (Camellia sinensis). The present study aimed to analyze the success of post-drought growth recovery of tea in response to different techniques and doses of biofertilizer applications. Field experiments were conducted from January to August 2024 in the experimental garden of the Research Institute for Tea and Cinchona, Gambung Blok A8, Bandung. This work was arranged in a split-plot design, consisting of a main plot with two biofertilizer application techniques (foliar feeding and soil drenching) and subplots with four levels of biofertilizer dosage (control (B1), 15 …

Abundance, Distribution, And Shoaling Of Driftfishes And Allies (Scombriformes: Stromateoidei) In The Pelagic Gulf Of Mexico, Juliet Tretler

All HCAS Student Capstones, Theses, and Dissertations

The suborder Stromateoidei contains 80 species of mesopelagic fishes in five families distributed throughout temperate and tropical seas worldwide, including the Gulf of Mexico (Gulf hereafter). Mesopelagic fishes, especially those that spatially aggregate, serve as important forage items for commercially, recreationally, and ecologically important species of fish, marine mammals, and seabirds. However, knowledge of the ecology and trophic importance of stromateoid fishes in the Gulf is extremely limited. This study provides quantitative information on the species composition, abundance, distribution, and biomass contribution of the stromateoid species to the Gulf deep-pelagic fish assemblage, with comparison to “archetypal” mesopelagic fish families (e.g., …

Effects Of Oil Spill Response Agents On Scleractinan Corals Acropora Cervicornis And Porites Divaricata, Austin Blakeslee

All HCAS Student Capstones, Theses, and Dissertations

Coral reefs are biodiverse coastal ecosystems at risk of exposure to spilled oil; spills reaching shorelines can damage corals, mangroves, and many other species in nearshore shallow-water tropical environments. Efforts to remediate such spills have evolved over the years through the introduction of various chemical response agents that efficiently and effectively control the spread of oil. Chemical herding agents and dispersants, which can contain and control the oil spread, are promising additions to the spill response toolbox as potential alternatives to other response methods. These response agents are designed to be biodegradable and minimize potential harm to aquatic ecosystems. However, …

Understanding The Effects Of Genetic Variation On Maize And Pennycress Root System Architectures And Their Interaction With Nitrogen, Alexander Euway Liu

Arts & Sciences Graduate Student Theses and Dissertations

Roots are a critical yet hidden part of plants, providing physical support and access to resources such as nutrients and water. The development of a plant’s root system across time and space is referred to as root system architecture, which influences the effectiveness of resource capture. In modern agriculture, applications of nitrogen fertilizers are critical to maintain high yield. However, up to 70% of applied nitrogen is not utilized by the plant and escapes into waterways and the atmosphere, causing environmental harm. Efficient nitrogen uptake is limited by the ability of the plant to access and absorb nitrogen through its …

Kabasura Kudineer Choornam, A Medicinal Polyherbal Formulation, Modulates Human Macrophage Polarization And Phagocytic Function, Asli Korkmaz, Duygu Ünüvar, Si̇nem Günalp, Derya Göksu Helvaci, Duygu Sağ

Turkish Journal of Biology

Background/aim: Kabasura kudineer choornam (KKC) is a polyherbal formulation of 15 ingredients. It has antiinflammatory and antimicrobial properties and are effective in managing the symptoms of H1N1 swine flu and COVID-19. However, its mechanism of action is not fully understood. In this study, we examined the effect of KKC on the polarization and function of primary human macrophages.

Materials and methods: Human monocyte-derived macrophages (M0 macrophages) pretreated with KKC extract were polarized into M1, M2a, or M2c subtypes. The expression of the M1/M2 polarization markers was analyzed using qPCR, flow cytometry, and ELISA, and the phagocytosis capacity of …

Mir-9-5p Alleviates The Development Of Abdominal Aortic Aneurysm By Regulating The Differentiation Of Cd4+Il-10+T Cells Via Targeting The Crosstalk Between Nrf2 And Nf-Κb Signaling Pathways, Hongfu Liu, Jinyi Zhang, Lubin Li, Benxiang Yu, Chunlei Zhang, Wenqiang Niu, Yawen Cheng, Hengyang Dong, Yukun Zhang, Xinlin Luo, Yanlian Xiong, Yueming Wang

Turkish Journal of Biology

Background: Abdominal aortic aneurysm (AAA), a gradual segmental dilatation of the abdominal aorta, is associated with a high mortality rate. The pathophysiological molecular mechanisms underlying AAA remain unclear. In recent years, changes in miRNA levels have been reported to be involved in the development and treatment of AAA. This study aimed to investigate the potential targets and underlying mechanisms of miR-9-5p in attenuating AAA progression by modulating the inflammatory response.

Materials and methods: Biochemical kits were used to measure the levels of inflammatory factors, antioxidant enzyme activity, and serum oxidative stress in normal and AAA model mice. miR-9-5p overexpression was …

Lactic Acid Bacteria Alleviate Neuronal Excitotoxicity And Extend Caenorhabditis Elegans Lifespan, Bao Le, Thi Nhat Hong Nguyen, Seung-Hwan Yang

Turkish Journal of Biology

Aim: Neuronal cell death plays a critical role in the development of neurological disorders associated with aging. This study aimed to evaluate the beneficial effects of heat-killed lactic acid bacteria (hkLAB) on neuroblastoma cells and Caenorhabditis elegans.

Materials and methods: We pretreated heat-killed Lactobacillus fermentum CNU384 (hkCNU384), L. brevis CNU386 (hkCNU386), L. helveticus CNU395 (hkCNU395), and L. paracasei CNU396 (hkCNU396) at a concentration of 10⁹ CFU/mL to investigate their neuroprotective effects on glutamate-induced SH-SY5Y cells and their impact on the lifespan of 6-hydroxydopamine (6-OHDA)-induced C. elegans.

Results: Our findings indicate that hkCNU395 and hkCNU396 protected against glutamate-induced cell death …

Chelidonium Majus L.-Containing Gel May Improve Diabetic Wound Healing By Modulating Mmp-2, Mmp-9, And Collagen Levels, Kaan Kaltalioğlu, Eli̇f Naz Gürsoy, Barbaros Balabanli, Zeki̇ Aytaç, Şule Coşkun Cevher

Turkish Journal of Biology

Background/aim: Delayed wound healing in diabetic patients is a significant complication that reduces quality of life, prompting the continuous investigation of new therapeutic agents. This study designed to explore the dose-dependent effects of different parts of Chelidonium majus L. (CM), a medicinal plant traditionally used for skin disorders, on diabetic skin wounds.

Materials and methods: In diabetic rats, full-thickness excisional wounds were formed. CM-containing gels (aerial parts at 3%, 6%, 9% and root at 0.5%, 1%, 1.5%) were developed and applied to the wounds. After the treatment period, the rats were sacrificed, and wound healing activity was assessed macroscopically, histopathologically, …

Book Reviews, William L. Hilsenhoff, Roger G. Bland, M. C. Nielson

The Great Lakes Entomologist

THE MAYFLIES OF NORTH AND CENTRAL AMERICA, George F. Edmunds, Jr., Steven L. Jensen, and Lewis Berner. 330 p., 432 figs. University of Minnesota Press, 1976. $28.50.

INSECTS AND THE LIFE OF MAN. Sir Vincent Wigglesworth. Halsted Press of John Wiley & Sons, N.Y. 217 p. $12.50.

A FIELD GUIDE TO THE BUTTERFLIES OF THE WEST INDIES. Normal D. Riley. A Demeter Press Book, Quadrangle-The New York Times Book Co., 1976. 224 pp., 24 colored plates. $12.50.

A Teratomorphic Stonefly (Plecoptera: Nemouridae), Allen W. Knight, Clifford A. Siegfried

The Great Lakes Entomologist

A single nymph of over 2000 specimens of Taeniopteryx nivalis collected in 1968 from Gull Creek, Kalamazoo County, Michigan (T25:R9W:S6), had a double flagellum on the left antenna (Fig. 1). The more laterally placed flagellum was perfectly formed and of normal length, whereas the inner one was less than half the normal length but appeared normally structured. The flagella shared a common pedicel.

Pollination Of Staghorn Sumac By Ants, Orin G. Gelderloos

The Great Lakes Entomologist

[Excerpt]

A population of worker ants, Prenolepis imparis Say (Hymenoptera: Formicidae), was observed pollinating the hermaphroditic flowers of staghorn sumac, Rhus nphina. at the University of Michigan-Dearborn in western Wayne County, Michigan, during the midmorning hours of 10 June, 1974, and at a height of approximately 1.75 m.

Color Variation In Strangalia Bicolor (Coleoptera: Cerambycidae), D. C.L. Gosling

The Great Lakes Entomologist

[Excerpt]

Many species of Lepturinae are noted for their great variation in color, while some are quite uniform in coloration. Strangalia bicolor (Swederus) is one of the Iatter type and usually shows virtually no variation. The elytra are uniformly blue-black with the exception of the inflexed corner of the humerus which is reddish-yellow. The wings and eyes are black, and the rest of the beetle is entirely reddish-yellow. Until recently, the only variation I had observed was one female with a small, triangular, reddish-yellow spot on the basal margin of each elytron near the scutellum. Linsley and Chemsak (1977) recorded …

Boyeria Grafiana, A Rare Ohio Dragonfly (Odonata: Aeshnidae), T. E. Perry

The Great Lakes Entomologist

[excerpt]

The intent of this paper is to record the dragonfly Boyeria grafiana Williamson as a resident Ohio species. The previous Ohio record for grafiana was based on a single male taken by E. E. Bogue at Orwell, Ashtabula County, Ohio, in September, 1894, and listed by Kellicott (1899) as Boyeria vinosa (Say). Hine (1913) updated some of Kellicott's work and identified the Orwell record as Boyeria grafiana, which had been described by Williamson in 1907.

Rev. Modestus Wirtner: Biographical Sketch And Additions And Corrections To The Miridae In His 1904 List Of Western Pennsylvania Hemiptera, A. G. Wheeler Jr., Thomas J. Henry

The Great Lakes Entomologist

[Excerpt]

The Rev. Modestus Wirtner's (1904) list of western Pennsylvania Hemiptera remains as the only useful general account of this group for the state. Earlier, Rathvon (1869) had published a short list of species from Lancaster County, but because the identity of species referred to cannot be established, his list has limited value.

The Distribution Of Some Soil Arthropods In A Manipulated Ecosystem, James H. Shaddy, James W. Butcher

The Great Lakes Entomologist

[Excerpt] Soil cultivation practices and agricultural utilization affect soil animal populations in various ways. Gisin (1955) and Schalk (1968) concluded that the effect was minimal. Others observed a deleterious effect (Karg, 1956; Sheals, 1956; Strebel, 1957; Aleinikova and Utrobina, 1975; Edwards and Lofty, 1975). However, some soil animals appear to be benefited (Aleinikova and Utrobina, 1975; Edwards and Lofty, 1975). The effects of cultivation and many other factors have been discussed thoroughly in reviews by Christiansen (1964), Burges and Raw (1967), Butcher, et al. (1971), Ghilarov (1975), and Wallwork (1976).

Survey For Parasites Of The Introduced Pine Sawfly, Dlprlon Slmllls (Hymenoptera: Diprionidae), In Minnesota, L. C. Thompson, H. M. Kulman, W. D. Valovage

The Great Lakes Entomologist

Diprion similis (Hartig) cocoons were collected at 24 sites in 14 counties in north and central Minnesota in 1975 to determine the current status of the parasite complex. The ichneumon Exenterus amictorius (Panzer) was by far the most abundant of the 16 parasites, with parasitization in excess of 44% in both sawfly generations. Parasitization by E. amictorius did not decrease substantially in the second generation, as was anticipated from previous studies. Monodontomerus dentipes (Dalman) (Torymidae) and Delomerista japonica diprionis Cushman (Ichneumonidae) were common only in the second generation. One specimen of Perilampus hyalinus Say (Perilampidae) was reared for the fust …

Distribution Of Acalymma Vittata And Diabrotica Virgifera (Coleoptera: Chrysomelidae) On Cucurbits, Catherine E. Bach

The Great Lakes Entomologist

The striped cucumber beetle, Acalymma vittata (Fabricius) and the western corn rootworm, Diahrotica virgifera LeConte, are of economic importance and utilize cucurbits, but to a different extent. A. vittata is a specialist on cucurbits during all stages of its life cycle, whereas D. virgifera feeds on cucurbits only as an adult. Much is known about the life history, feeding preferences, and control of each of these species (A. vittata: Gould, 1944, 1962; Harcourt and Cass, 1955; Wiseman, Hall, and Painter, 1962; D. virgifera: Chiang, 1973; Ludwig, 1975). However, no study has looked at possible interactions such as competition between the …

Invertebrate Predators Of Michigan Lepidoptera, M. C. Nielson

The Great Lakes Entomologist

[Excerpt]

For many years this author has been actively collecting Michigan Lepidoptera and generally observing insects in the field. Almost every season some predation of Lepidoptera by insects and their allies is seen. Unfortunately, many early observations went unrecorded in my field notebooks, but since the late 1950's I have made notations of these interesting encounters.

A Distinctive Dune Form Of The Marbled White Butterfly, Euchloe Olympia (Lepidoptera: Pieridae) In The Great Lakes Area, Warren Herb Wagner Jr.

The Great Lakes Entomologist

[Excerpt]

The dunes along the shores of Lake Huron in the vicinity of Ipperwash Provincial Park, Port Franks, and Pinery Provincial Park, Ontario, are notable for their unusual vegetation and a number of interesting plants and animals. During studies of the peculiar, disjunctive populations of the dwarf hackberry tree (Celtis tenuifolia Nutt.) (Wagner, 1974), I became aware that the local population of marbled white, Euchloe olympia (Edwards), differed in a number of respects from the usual form with which I was familiar. Preliminary observations during the spring of 1975 suggested that the butterflies might even represent a distinct subspecies, so …

Development And Survival Of Saratoga Spittlebug Nymphs On Alternate Host Plants, Louis F. Wilson, George C. Heaton, Patrick C. Kennedy

The Great Lakes Entomologist

\!hen abundant, the adult Saratoga spittlebug, Aphrophora smatogensis (Fitch), injures or kills young red pine, Pinus rerinovr Ait., and jack pine, P. banksiana Lamb. Its abundance depends greatly on the quantit). and quality of woody plants and forbs of the forest floor that senre a5 alternate hosts during the nymphal stages (Anderson, 1947; Ewan, 1961: Kennedy and Wilson, 1971; Secrest, 1944). Sweet-fern, Comptonia peregrina Coult., is the principal and most important alternate host (Kennedy and Wilson, 1971), but little is know-n about the value of other woody plants and forbs for supporting nymphal development. Therefore, \ve sought to examine the …

Parasites Of Lepidopterous Larvae In Alfalfa And Soybeans In Central Illinois, S. J. Roberts, W. K. Mellors, E. J. Armbrust

The Great Lakes Entomologist

During the 1971-72 soaing season sweep-net samples were taken to determine the major species of parasites and lepidopterous host larvae present in central Illinois soybeans and alfalfa. A trod of 27 primary Hymenoptera and Diptera parasites was collected from nine Lepidopterous species. Parasites were associated with all nine species of Ian-ae m alfalfa and soybeans. Apanteles flaviconchae Riley was responsible for over 87% of &e parasitism of Colias spp. larvae in both alfalfa and soybeans in 1971. The hyperparasites Mesochorus discitergus (Say) and M. arnericanus Cresson were recovered from fou~of dep rim= parasites Pathogens were found in six species of …

Temporomandibular Joint Osteoarthritis And Systemic Inflammation: A Cadaveric Study For Dental Insights, Nathan Kent Bs, David Patterson Bs, Alyssa Luu Bs, Samantha Pipkin Bs, Jason Ciccotelli Dpt, Phd, Jeremy James Dmd, Ms, Jessica Immonen Phd, Ms

The Journal of Integrative Physiology

The objective of this study was to determine if gross signs of cadaveric osteoarthritis (OA) have

a tendency to present systemically and if this presentation includes pathology found at the

temporomandibular joint (TMJ). Signs of OA were then correlated to gross alterations of the aorta and common carotid arteries seen with atherosclerotic cardiovascular disease. Ipsilateral synovial joints including the TMJ, knee joint, radiocarpal joint and trapeziometacarpal (TMC) joint were all assessed for signs of OA in 4 cadaveric donors (3 males; 72, 82 and 92 years-old / 1 female; 89 years-old) using a validated scale of disease severity. A Grade …

The Legal System For Influencers On Social Media Platforms: A Comparative Study, Ahmad Ibrahim Ballal, Najem Riyad Al-Rabadi, Ma’Moun Rashed Al-Huneiti

Middle East Journal of Communication Studies

This study explores the legal regulation of social media influencers through a comparative analysis between French and Jordanian legislation. The research problem lies in the absence of a clear and comprehensive legal framework regulating influencers' growing role in shaping public opinion. Using the comparative analytical method, the study examines relevant legal texts and regulatory approaches in both jurisdictions. The findings highlight significant differences, with France enacting a specific law to govern influencer activities, while Jordan still relies on general legal provisions. The study recommends drafting a dedicated Jordanian law that outlines influencers' legal responsibilities and balances freedom of expression with …

Representations Of The Other In Hamas's Media Discourse During The "Al-Aqsa Flood" Operation: A Rhetorical Analytical Study, Enad Mamdouh Al-Zaben, Sabah Al Harahsheh

Middle East Journal of Communication Studies

This study aimed to determine how the Hamas movement represented the "other" in its media discourse during the "Al-Aqsa Flood" operation. To achieve this goal, the study relied on the qualitative analytical approach, specifically rhetorical discourse analysis. The study sample included all Hamas's media spokesman speeches, between October 7, 2023, and January 1, 2024, numbering 11 speeches. The study results showed that Hamas's media discourse focused on portraying two main groups: "us" and "them." The "us" group included the Palestinian people in all their spectra. They were referred to using terms with positive connotations such as: "the steadfast," "the one …