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Chemically Mediated Communication In Mound-Building Termites, Sree Subha Ramaswamy

Theses and Dissertations

Nest-building in social insects requires layered communication to coordinate complex, functional structures for housing, protection, storage, and homeostasis. This is especially striking in mound-building termites, which construct and repair large mounds collectively despite lacking image-forming eyes. This thesis investigates how Odontotermes obesus achieves such coordination through chemical communication embedded in soil.

Using controlled two-choice assays with manipulated soils, I separated airborne volatile signals from contact non-volatile signals by baking to remove volatiles, a mesh assay that presented volatile cues without contact, and solvent extraction with reapplication of contact cues. I quantified responses in major-only, minor-only, and mixed groups at two …

Low-Tech Seed Enhancement Technology Could Improve Seedling Establishment Of Longleaf Pine Understory Perennial Forbs, Madeline E. Schmuck, Michael C. Disilvestro, Danny J. Gustafson

Journal of the South Carolina Academy of Science

Reintroducing native plants into restoration and revegetation projects could increase plant species diversity, increase floral resources for pollinators, and positively affect trophic-level interactions in the community. Direct seeding in restoration projects has generally had poor success rates due to biotic and abiotic factors, leading to significant seed wastage of the already insufficient native seed supply. Seed balls (aka seed aggregates, seed bombs, and seed pellets) are a low-cost, low-tech seed enhancement technology. The seeds are encased in a soil and clay matrix, providing good seed-soil contact, controlling seed planting depth, nutrients, and protection from seed predators. We conducted a greenhouse …

Long Intergenic Non-Coding Rna 00511 (Linc00511) Genetic Variations And Haplotype Implication For Colorectal Cancer Susceptibility And Prognosis, Eman Fouad Sanad, Ahmad Abd El Hady, Mohamed Ali, Shorouk Eldash, Nermeen H. Elmorshedy, Farah Ayman, Hams M. Khattab, Amr Maher, Hadeel Ashree, Mahitab Abdelhady, Mazen Mohamed, Ahmed Adel, Sajed Khalil, Omar Alyan, Ahmed Samir, Alhassan A. Bakr, Nadia Hamdy

Pharmacy

Long intergenic non-coding RNA 00,511 (LINC00511) is considered an oncogene for various cancers. However, the association between LINC00511 single nucleotide polymorphisms (SNPs) and colorectal cancer (CRC) remains unclear. Our study aims to study whether LINC00511 SNPs could predict CRC susceptibility or prognosis, an important step-toward precision-health, based on an Egyptian CRC patient cohort. A total of 200 CRC patients and 200 cancer-free controls were genotyped for three LINC00511 SNPs − rs9906859, rs1558535, and rs17780195 using qRT-PCR. Studied SNPs were in strong linkage disequilibrium and moderately correlated in all groups. Genotype association concerning tumor stage, revealed rs1558535 AT and rs17780195 AG …

08.11.2025 Ored Connect, Liz Williamson

ORED Newsletter

F and A rates

ORED Research Reception

NSF Audit Final Resolution and Compliance Updates

Impact Of Urban Fine Particulate Matter Exposure On Cardiovascular Health, Shelby P. Briggs

Honors Theses

Air pollution, particularly exposure to fine particulate matter (PM2.5), poses a growing threat to cardiopulmonary health. PM2.5 consists of microscopic particles smaller than 2.5 microns in diameter and originates from industrial combustion, transportation, and urban dust. These particles are classified as xenobiotics and are capable of bypassing respiratory defenses to enter systemic circulation, where they cause oxidative stress, inflammation, and cardiovascular dysfunction. This study examines the effects of PM2.5 on cardiovascular function using a murine model. Male C57BL/6 mice were divided into control (saline), subchronic (10 µg), and chronic (100 µg) groups and were exposed weekly for 11 weeks via …

Successful Artificial Incubation And Juvenile-Rearing Of Dropped Eggs Of A Critically Endangered Freshwater Crayfish (Cherax Tenuimanus), Rodney Duffy Dr., Blaine Hodgson, Amber Quinn, Marcus Newman

Fisheries Research Articles

One-third of the world’s freshwater crayfish species face extinction risk, necessitating extreme and urgent conservation measures such as captive rearing. Little information exists on these measures for most threatened species. The hairy marron, Cherax tenuimanus (Parastacidae), is a critically endangered freshwater crayfish endemic to the Margaret River in south-western Western Australia. C. tenuimanus is being rapidly replaced by the smooth marron, Cherax cainii, a translocated congener, causing its progressive decline in range, and now it faces imminent extinction in the wild. Attempts at captive breeding have resulted in few occurrences of egg laying of C. tenuimanus, which ended in complete …

An Rna Modification Prevents Extended Codon-Anticodon Interactions From Facilitating +1 Frameshifting, Evelyn M. Kimbrough, Ha An Nguyen, Haixing Li, Jacob M. Mattingly, Nevette A. Bailey, Wei Ning, Howard Gamper, Ya-Ming Hou, Ruben L. Gonzalez, Christine M. Dunham

Department of Biochemistry and Molecular Biology Faculty Papers

RNA post-transcriptional modifications act by stabilizing the functional conformations of RNA. While their role in messenger RNA (mRNA) decoding is well established, it is less clear how transfer RNA (tRNA) modifications outside the anticodon contribute to tRNA stability and accurate protein synthesis. Absence of such modifications causes translation errors, including mRNA frameshifting. By integrating single-molecule fluorescence resonance energy transfer and cryogenic electron microscopy, we demonstrate that the N1-methylguanosine (m1G) modification at position 37 of Escherichia coli tRNAProL is necessary and sufficient for modulating the conformational energy of this tRNA on the ribosome so as to suppress +1 frameshifting otherwise induced …

High Temperature In Potato: Plant Responses And Adaptive Cultivation Strategies To Increase Production, Siska Rahmayani Gultom, Jajang Sauman Hamdani, Kusumiyati

Jurnal Kultivasi

Climate change, with global temperatures rising by 1.09°C from 1850–1900 to 2011–2020, threatens potato production, a critical staple crop, by exceeding the optimal temperature range of 15–20°C. This review synthesizes over 45 peer-reviewed studies published between 2015 and 2025 from Google Scholar and ScienceDirect to evaluate the physiological, morphological, and tuber quality responses of potatoes to high temperatures and to identify adaptive cultivation strategies for sustainable production. High temperatures reduce photosynthetic efficiency through chlorophyll degradation and stomatal closure, increase respiration, and divert photosynthates to vegetative growth, leading to 18–32% yield losses globally by the 2050s. Heat-tolerant varieties, such as Atlantic …

Effect Of Potassium Fertilizer On The Nutrition And Physical Quality Of Job’S Tears (Coix Lacryma-Jobi L.) Seeds, Qinthara Nail Haysa, Yuyun Yuwariah, Fiky Yulianto Wicaksono

Jurnal Kultivasi

Job's tears is a cereal crop that has beneficial nutrients. Potassium is known to affect the growth and yield of cereal crops, but there has been limited study on whether potassium affects the nutrition and physical quality of Job’s tears seeds. This study aimed to analyze the effect of K fertilizer on the nutritional content and physical quality of Job's tears seeds. This research was conducted from January to May 2024 at Ciparanje Experimental Field, Faculty of Agriculture, Universitas Padjadjaran, Sumedang, Indonesia. This experiment used the Randomized Block Design (RBD) method with six treatments and four replications. Various doses of …

Hippocampal Avoidance During Prophylactic Cranial Irradiation For Patients With Small Cell Lung Cancer: Randomized Phase Ii/Iii Trial Nrg-Cc003, Vinai Gondi, Stephanie L Pugh, Minesh P Mehta, Jeffrey S Wefel, Wolfgang A Tomé, Alexander Y Sun, John Grecula, Kristin J Redmond, Shannon Fogh, Laurie Gaspar, Andre Konski, Joseph Bovi, Clifford G Robinson, Benjamin Corn, Gregory M Videtic, Benjamin H Lok, Harold A Yoon, John H Heinzerling, Albert S Denittis, Ronald C Mcgarry, Kiran Devisetty, Vijayananda Kundapur, Abraham J Wu, Edward C Mccarron, Isabelle Thibault, Edmund L Simon, Andrew M Baschnagel, Samir Narayan, Jondavid Pollock, Rebecca Paulus, Lisa A Kachnic

Faculty, Staff and Student Publications

Purpose: Hippocampal avoidance (HA) during therapeutic whole-brain radiotherapy reduces the risk of neurocognitive function (NCF) toxicity in patients with brain metastasis. This trial hypothesized that HA during prophylactic cranial irradiation (PCI) in patients with small cell lung cancer (SCLC) leads to noninferior intracranial relapse (ICR) and reduction in NCF toxicity.

Methods: This randomized phase II/III trial enrolled patients with SCLC, no brain metastases, and response to chemotherapy. The primary end points were 12-month ICR (noninferiority design, randomized phase II) and 6-month Hopkins Verbal Learning Test-Revised (HVLT-R) Delayed Recall (DR) failure (phase III). Secondary end points were failure in any NCF …

Health Outcomes Beyond Age 50 Years In Survivors Of Childhood Cancer: A Report From The Childhood Cancer Survivor Study, Rusha Bhandari, Yan Chen, Eric J Chow, Rebecca M Howell, Lisa B Kenney, Kevin R Krull, Wendy Leisenring, Paul C Nathan, Joseph P Neglia, Kirsten K Ness, Kevin C Oeffinger, Claire Snyder, Lucie M Turcotte, F Lennie Wong, Yutaka Yasui, Gregory T Armstrong, Saro H Armenian

Faculty, Staff and Student Publications

Purpose: There are limited data on the risk for mortality and health outcomes among the increasing population of older (age >50 years) survivors of childhood cancer during this later stage in life when there is an expected increase in aging-related morbidities.

Methods: We assessed cause-specific mortality, incident new cancers, chronic health conditions (CHCs), frailty, and health status among survivors from the Childhood Cancer Survivor Study, conditional on surviving to 50 years. We calculated conditional survival rates, standardized mortality ratios (SMRs), and, for incident new cancers, cumulative burden, standardized incidence ratios (SIRs), and relative rates (RRs), compared with the general US …

Keap1 And Stk11/Lkb1 Alterations Enhance Vulnerability To Atr Inhibition In Kras Mutant Non-Small Cell Lung Cancer, Ana Galan-Cobo, Natalie I Vokes, Yu Qian, David Molkentine, Kavya Ramkumar, Alvaro G Paula, Marlese Pisegna, Daniel J Mcgrail, Alissa Poteete, Sungnam Cho, Minh Truong Do, Amirali Karimi, Yifan Kong, Anisha Solanki, Ankur Karmokar, Nicolas Floc'h, Adina Hughes, Rebecca Sargeant, Lucy Young, Li Shen, Gozde Kar, Caezaan Keshvani, Claudio Arrechedera, Sharia Hernandez, Katharina Schlacher, Jing Wang, Sonia Iyer, James Conway, Mohamed Reda Keddar, Marta Milo, Ilario De Toma, Susan E Critchlow, J Carl Barrett, Jan Cosaert, Alan Lau, Viia Valge-Archer, Lauren A Byers, Simon T Barry, John V Heymach

Faculty, Staff and Student Publications

KRAS mutations frequently co-occur with alterations in STK11/LKB1 and/or KEAP1, defining an aggressive subset of lung cancers resistant to immuno- and chemotherapy. While LKB1 loss is associated with vulnerability to DNA damage response-based therapies, the impact of KEAP1 alterations remains unknown. We demonstrate that KEAP1-NRF2 pathway drives a compensatory modulation of ATR-CHK1 signaling, enhancing vulnerability to ATR inhibitors (ATRi), particularly in the setting of increased replication stress associated with LKB1 loss. ATRi shows enhanced anti-tumor activity in LKB1 and/or KEAP1-deficient non-small cell lung cancer (NSCLC) models and synergizes with gemcitabine. ATRi also enhances antitumor immunity and mitigates the immunosuppressed phenotype …

Agricultural Biotechnology In The Courts: Judicial Opinions And Commentary, Drew L. Kershen

Faculty Articles

Seven jurisdictions from around the world have issued judicial opinions that address fundamental issues about the governance and regulatory systems of agricultural biotechnology. This article summarizes these legal proceedings and describes their impact upon agricultural biotechnology. The article then provides a commentary and critique of the legal proceedings and resulting judicial opinions.

Polo-Like Kinase 1 Inactivation Enhances Pi3k Inhibition-Mediated Apoptosis Of Notch1-Mutant Head And Neck Squamous Cell Carcinoma, Pooja A Shah, Tuhina Mazumdar, Soma Ghosh, Lacin Yapindi, Reid T Powell, Yong S Park, Li Shen, Anne M Fernandez, Clifford C Stephan, Jing Wang, Andrew G Sikora, Jawad Kazi, Mitchell J Frederick, Faye M Johnson

Children’s Nutrition Research Center Staff Publications

PI3K inhibition causes apoptosis selectively in NOTCH1-mutant head and neck squamous cell carcinoma (HNSCC), but modest single-agent responses and acquired resistance (AR) limit the clinical efficacy of targeted agents. To address these limitations, we investigated novel combination therapies. We tested the efficacy of 5768 compounds as single agents and 139 in combination with PI3K inhibitors in sensitive and AR NOTCH1-mutant HNSCC cell lines. We generated synergy/efficacy classifications for the combinations using multiple metrics of statistical drug synergy and growth rate indices. The PLK1/PI3K combination's efficacy was validated using orthogonal in vitro methods and in two HNSCC xenograft models. Compound efficacy …

Mutant P53 Confers Chemoresistance By Activating Kmt5b-Mediated Dna Repair Pathway In Nasopharyngeal Carcinoma, Haidan Luo, Mo-Fan Huang, An Xu, Donghui Wang, Julian A Gingold, Jian Tu, Ruoyu Wang, Zijun Huo, Yen-Ting Chiang, Kuang-Lei Tsai, Jie Su, Danielle A Bazer, Mien-Chie Hung, Canmao Xie, Yubiao Guo, Dung-Fang Lee, Huiling Yang, Ruiying Zhao

Faculty, Staff and Student Publications

Nasopharyngeal carcinoma (NPC), a malignancy arising from the nasopharyngeal epithelium, is common in the east and southeast area of Asia. Treatments for locally advanced and recurrent NPC include chemotherapy (usually combined with 5-Fluorouracil, 5-FU) and radiotherapy, but response is limited due to chemo-resistance. p53 mutation is a critical factor for 5-FU resistance in some cancers, but its role in NPC chemo-resistance remains unclear. Here, we demonstrate that p53(R280T), a common p53 somatic mutation found in multiple NPC tumor samples, induces gain-of-function upregulation of DNA repair genes which leads to 5-FU resistance in NPC. p53(R280T) specifically upregulates the expression of DNA …

Development Of A Cure In Principles Of Cell And Molecular Biology, Heather Seitz

Sabbatical Projects

This project presentation highlights the work done during sabbatical to develop an authentic research experience into BIOL135 Principles of Cell and Molecular Biology. The sabbatical allowed for development time but further work was done to measure impact and outcomes for future implementation.

Tracking The Type Of Specimens Of Parazoanthus Axinellae (Hexacorallia: Zoanthidea) And Its Subspecies, Annika Markovich

DoMES Seminar Series

No abstract provided.

Exploring Changes In Body Composition Before And After A National Collegiate Athletic Association Division I Football Season Of Marching Artists, Noelle J. Smith, Brett Nickerson, Ronald L. Snarr, Matt P. Brancaleone

International Journal of Exercise Science

International Journal of Exercise Science 18(8): 727-735, 2025. The purpose of this study was to observe the changes in body composition of collegiate marching band artists after a competitive season NCAA Division I football season. Thirty-seven marching artists (7 females, 31 males; age: 21.5 (1.4) years; height: 177.2 (9.5) cm had body fat percentage (BF%), fat mass (FM), android adiposity, gynoid adiposity, bone mineral content (BMC), and lean soft tissue (LST) measured before and after a competitive season of Division I football. Body composition measures were conducted using single-frequency bioelectrical impedance analysis device (Quantum IV, RJL systems, Clinton MI). Pre- …

Shimmering Mirage Or A Promised Land? Climate Change, Agriculture, And Internal Migration In Sabha, Jordan, Tyler J. Rak

Capstone Collection

Given the rapidly shifting climate, high vulnerability, and low adaptability to climate related changes that rural areas face, this study highlights the complex relationship between climate change -- especially water scarcity -- agriculture, and internal migration in rural Jordan. I predict that if water scarcity is present and climate changes are occurring, they will undercut the rural agriculture sector which could contribute to rural out-migration. To study this relationship, I conducted a case study in Sabha, a small rural community in the Northern Badia of Jordan. Data was gathered by engaging in semi-structured interviews with current community members in Sabha …

From Larvae To Livelihoods: Examining The Realities Of Black Soldier Fly Farming In Malawi, Emily Herriott

Capstone Collection

Black Soldier Fly (BSF) larvae offer a cost-effective, nutrient-rich alternative to synthetic fertilizers and conventional feed inputs such as soy, fishmeal, and maize bran in sub-Saharan Africa. In Malawi, where smallholder farmers grapple with declining soil fertility, high input prices, chronic food insecurity, and livestock nutritional challenges, BSF farming is emerging as a promising circular agricultural innovation. Despite its technical promise, the social, economic, and ecological realities of BSF adoption remain underexplored.This study investigates the early implementation of BSF farming in Malawi through the Sustainable Livelihoods Framework, focusing on farmer experiences, challenges, and the social and structural processes shaping uptake …

Integrating Neuroaesthetic Design Into Brain-On-Chip Research: Toward Enhanced Neural Engagement And Cognitive Accessibility, Piper Hutson, James Hutson

Faculty Scholarship

This study examines the potential for neuroaesthetic design and adaptive neurofeedback principles to enhance brain-on-chip (BoC) research, with an emphasis on optimizing neural engagement and cognitive accessibility for diverse populations. Existing BoC platforms have transformed neuroscience through high-fidelity modeling of neural circuits, yet cognitive accessibility and sensory inclusion remain comparatively underexplored. Drawing upon recent studies in neuroinclusion, multisensory interface design, and adaptive feedback, this work synthesizes findings from biophilic interface architecture, neurofeedback literature, and embodied cognition frameworks. The manuscript outlines a multidisciplinary research agenda that prioritizes sensory diversity and ethical considerations in the evolution of BoC systems. Instead of making …

Growth And Yield Of Curly Red Chili (Capsicum Annum L.) In Response To Mulching And Chicken Manure Application In The Erosion-Prone Area Of Loto, Ternate Island, Tri Mulya Hartati, Lily Ishak, Krishna Aji, Chumidach Roini

Jurnal Kultivasi

Information on soil resources in volcanic landscapes presents a high potential for agricultural land development, but its development is always faced with the potential for continuous land degradation. This study aims to reduce the rate of land degradation through the application of vegetative conservation in erosion-prone Loto agrotourism area, Ternate Island. The research location is focused on erosion-prone areas. The research consisted of a soil resource inventory and a vegetative conservation application. The vegetative conservation application method used a Randomized Block Design (RBD) pattern, composed of 2 factors. The first factor is mulching, namely: no mulching (M0) and mulching (M1), …

Dairy And Food Science Student Newsletter, August 8, 2025, Department Of Dairy And Food Science

Dairy and Food Science Student Newsletter

No abstract provided.

Genetic And Environmental Factors Contributing To The Pathogenesis Of Vertebral Osteomyelitis Caused By Enterococcus Cecorum In Broiler Chicken, Ummu Syauqah Al Musyahadah, Andi Asnayanti, Anh Dang Trieu Do, Adnan Alrubaye

Poultry Science Faculty Publications and Presentations

Enterococcus cecorum (EC) is the primary cause of Vertebral Osteomyelitis (VO) in poultry, characterized by a hock-sitting posture with legs extended forward. The disease begins with intestinal colonization, where pathogenic strains of E. cecorum establish themselves in the gut within the first week of a bird's lifecycle and translocate to the bloodstream later, reaching the organs, which finally infect the birds' Free Thoracic Vertebrae (FTV). Several factors contribute to the initiation and progression of the disease. Environmental factors such as heat stress, high blood sugar levels, prior infections, overcrowding, and rapid growth conditions weaken immunity and stimulate the emergence of …

The Effect Of Feed And Water Provision Strategies On Broiler Breeder Pullet Performance And Welfare, Allison D. Weaver, Lisa R. Bielke, Ramon D. Malheiros, Sara K. Orlowski, Allison N. Pullin

Poultry Science Faculty Publications and Presentations

Feed restriction is common in the broiler breeder industry to optimize health and reproduction. However, this practice has been associated with increased drinking behavior, leading to water spillage, higher litter moisture, and footpad lesions. Consequently, parts of the industry have adopted water restriction protocols. This study aimed to evaluate how different combinations of feed and water restriction affected drinking behavior, welfare, and performance indicators in broiler breeder pullets. At 1 day of age, 960 Cobb 500 FF pullets (Gallus gallus domesticus) were randomly allocated to one of four treatments: skip-a-day feeding with ad libitum water (SAD + ADLIB), every-day feeding …

Northern Arizona Ecological Conservation: Assessing Quaking Aspen Health In Northern Arizona Using Earth Observations, Ikram Morso, Luke Collins, Margaret Cox, Melissa Schwan

Aspen Bibliography

Quaking aspen (Populus tremuloides) forests in Northern Arizona provide critical habitat for endangered bird species and support regional tourism and recreation. However, aspen populations are declining due to a combination of abiotic and biotic stressors. In response, land managers implemented strategies, such as ungulate exclusion fencing, prescribed burns, and stand thinning, but efforts are constrained by limited data on where and when to intervene. This project worked in collaboration with the U.S. Forest Service, the National Park Service, the Arizona Department of Forestry and Fire Management, and Northern Arizona University School of Forestry. The team utilized Earth observations …

Thionine Modulates Tau Phosphorylation In An Alzheimer’S Disease Cell Culture Model, Seda Önder, Kevser Bi̇beroğlu, Özden Tacal

Turkish Journal of Biology

Background/aim: Tau protein, which is crucial for sustaining the cytoskeletal network by assisting microtubule construction, contributes significantly to the pathophysiology of Alzheimer’s disease (AD). The hyperphosphorylation of tau causes it to detach from microtubules (MTs), leading to the formation of neurofibrillary tangles (NFTs) in neurons, which ultimately results in cell death. Thionine (TH), a cationic phenothiazine-structured compound, has been the topic of extensive research due to its interesting physicochemical properties. It is a common biological dye, especially useful in histology due to its strong affinity for biological membranes. Furthermore, TH serves as a photosensitizer in phototherapy. It has a phenothiazine …

Rice (Oryza Sativa L.) Production, Productivity, And Harvested Area In Karawang Regency Under Extreme Weather From 1991-2023, Adine Syabina Buswar, Ruminta, Fiky Yulianto Wicaksono

Jurnal Kultivasi

Climate change occurred in Karawang Regency due to extreme weather events, which are included in the El Nino climate anomaly phenomenon. The existence of extreme weather events in Karawang Regency has decreased rice production, which is a climate-dependent crop. Based on this problem, a study was conducted to analyze the identification of extreme weather changes and correlation analysis of rice crops in Karawang Regency to see the magnitude of extreme weather changes and the influence of extreme weather elements on rice crops. The method used is a quantitative descriptive method by analyzing extreme weather changes, correlation analysis of extreme weather …

Effect Of Preharvest Paclobutrazol And Nitrogen Fertilizers On The Sprouting Performance Of ‘Median’ Potato Seed G0 Tuber, Jajang Sauman Hamdani, Rahmat Budiarto, Anne Nuraini, Selika Fitrian Ramadani

Jurnal Kultivasi

Various intensifications of potato cultivation, such as the provision of paclobutrazol and nitrogen (N) fertilizer, are thought to impact the quality of the seeds produced. This study aims to evaluate the effects of different N fertilizer doses and paclobutrazol concentrations applied in the preharvest period on the sprouting performance of G0 potato tuber seeds after storage. Nine treatment combinations, each repeated three times, were tested, using 50%, 100%, and 150% of the recommended N dose and paclobutrazol concentrations of 50, 100, and 150 part per million (ppm), applied at 30 and 45 days after planting, respectively. The interaction effect between …

Investigating Food Web Dynamics Of Great Lakes Coastal Wetlands Using A Novel Stable Isotope Approach, Alyssa M. Smith

Masters Theses

Coastal wetlands of the Great Lakes support abundant populations of fish, invertebrates, and vegetation, though the trophic linkages connecting primary production and lower consumers is not well understood in these systems. We implemented a multiple-tracer approach, pairing traditional food web isotope tracers like carbon (δ13C) and nitrogen (δ15N) with total mercury concentrations (THg). We predicted that filamentous algae would be the dominant energy resource in the diet of lower trophic-level invertebrates in the Grand River Estuary, a network of riverine coastal wetlands adjacent to lake Michigan. In addition, we predicted that adding THg as a tracer would improve the resolution …