Digital Commons Network^™

Effects Of Gamma Irradiation On Antioxidant Enzyme Activities In Black Carrot Callus Under Drought Stress, Damla Turan Büyükdi̇nç, Di̇lruba Tabay Sümbül, Sheikh Mansoor

Turkish Journal of Agriculture and Forestry

Daucus carota L. (carrot) is a commercially significant root vegetable widely cultivated in temperate regions for its edible taproot and valued for its high nutritional content. This study aimed to investigate the effects of drought stress on callus tissues of the Hatay black carrot (Daucus carota L. ssp. sativus var. atrorubens Alef.) genotype and to determine the impact of different gamma radiation doses on antioxidant enzyme activities. Black carrot callus tissues were exposed to gamma irradiation at doses of 0, 7, 8, and 9 Gy to evaluate their responses to drought stress. Antioxidant enzyme activities, including superoxide dismutase (SOD), …

Bioactive Profile And Mineral Composition Of Fruit Extracts From Four Wild Hawthorn (Crataegus Spp.) Species, Özlem Çakir, Kübra Çinar Topçu, Abdurrahman Sefali, İshak Bayyi̇ği̇t, Gülçe İlhan, Jyoti̇ Singh

Turkish Journal of Agriculture and Forestry

This study compared the antioxidant capacity, total phenolic content (TPC), phenolic composition, lipid oxidation level, andmineral profiles of extracts obtained from four wild hawthorn species (Crataegus tanacetifolia, C. orientalis subsp. orientalis, C. orientalissubsp. szovitsii, and C. monogyna) collected from northeastern Türkiye. Significant interspecific variations were observed in DPPH,FRAP, and CUPRAC assays. While C. monogyna had the highest reducing capacity and TPC, C. orientalis subsp. szovitsii had the highestDPPH activity. Ultra-high performance liquid chromatography coupled to tandem mass spectrometry analysis showed that catechin,epicatechin, chlorogenic acid, and quinic acid were the major phenolics, and were particularly abundant in C. monogyna. Despitehaving a …

Production Of The Antitumor Compound Deoxyharringtonine By Suspension-Culturedcells Of Cephalotaxus Hainanensis, Liang Wang, Muhammad Mubashar Zafar, Chongyu Wu, Zhiqiang Xiong, Jingyi Sun, Huapeng Sun, Hanqing Cong, Xuefei Jiang, Mahmoud F. Seleiman, Fei Qiao, Peter Nick

Turkish Journal of Agriculture and Forestry

Plant cell fermentation can be a sustainable biotechnological alternative to the wild collection of medicinal plants. As aparadigm, we explored the potential of using the highly endangered endemic tree Cephalotaxus hainanensis, which accumulatescephalotaxine esters—potent anticancer compounds. We successfully established a suspension cell line able to convert cephalotaxineinto its esters, including deoxyharringtonine, harringtonine, isoharringtonine and homoharringtonine at a remarkable maximalconversion rate of up to 86%, when supplemented with L-homoleucine, a precursor for the side chains of these esters. Furthermore, byadding elicitors, we were able to enhance the yield significantly beyond the values expected from the complete conversion of the addedcephalotaxine. In …

Pest Management For Utah Cut Flower Production: Insects And Their Relatives, Nick Volesky, Marion Murray, Melanie Stock

All Current Publications

Pest management is important in cut flower production, marketability, and farm profitability. This fact sheet focuses on animal pests (insects, arthropods, mollusks, and vertebrates), not diseases or weeds, and has two parts. Part 1 applies Integrated Pest Management (IPM): using preventive techniques, pest thresholds, and best management practices with pests in cut flower crop production. Part 2 highlights the top 10 common pests of cut flowers in Utah and their management.

Adjunctive Hyperbaric Oxygen Therapy For Chronic Diabetic Foot Ulcer Unresponsive To Standard Care: A Case Report, Ismail Ismail, Sukriyadi Adi, Sitti Rahmatia, Agustan Agustan

BioMedicine

Background: Diabetic foot ulcers (DFUs) are a serious complication of diabetes mellitus and are often challenging to treat, particularly in patients who fail to respond to standard wound care. Adjunctive therapies such as hyperbaric oxygen therapy (HBOT) have shown potential in promoting wound healing in chronic cases.

Case Presentation: A 66-year-old male with a 10-year history of type 2 diabetes mellitus presented with a chronic, non-healing DFU on the right foot. Despite oral antibiotic therapy and conventional wound management, the ulcer demonstrated progressive necrosis, persistent infection, and tissue exposure. Wound culture identified Klebsiella oxytoca. After targeted antibiotic treatment, the patient …

Neurosurgical Management Of Pineal Lesions: Insights From A Single-Center Study Of 27 Cases, David Montesinos Contreras, Walter Stummer, Maryam Khaleghi Ghadiri

BioMedicine

Introduction: Pineal lesions are rare and surgically challenging due to their deep location, histological diversity, and potential malignancy. The complexity of the pineal region anatomy and the diverse pathological spectrum contribute to the lack of standardized treatment strategies, making appropriate management controversial. Aim: This study aimed to evaluate the surgical outcomes of patients with pineal tumors or cysts and to assess the impact of different surgical approaches, particularly the extent of resection, on progression-free survival. Methods: We conducted a retrospective analysis of 27 patients treated for pineal tumors or cysts at our institution between 2010 and 2020. Data regarding clinical …

A Glimpse Into The Impact Of Physical Activity On Linear Growth In Children And Adolescents Through Activity Restrictions Accompanying The Covid-19 Outbreak, Yi-Chun Lin, Wen-Ling Liao, Chung-Hsing Wang, Fuu-Jen Tsai

BioMedicine

Background: Linear growth in children and adolescents, a key health indicator, is influenced by various factors, including physical activity (PA). The COVID-19 lockdown in Taiwan created a unique opportunity to examine the effect of PA restrictions on growth.

Methods: This retrospective cohort study was conducted at two hospitals in central Taiwan. The study included 72 children and adolescents (26 males, 46 females) who attended a growth clinic. Height and weight measurements were recorded in three stages: pre-lockdown, during lockdown, and post-lockdown. Growth rates were compared across stages using paired t-tests.

Results: The average growth rate was lowest during lockdown (0.0181 …

Assessing The Capabilities Of Ai-Based Large Language Models (Ai-Llms) In Interpreting Histopathological Slides And Scientific Figures: Performance Evaluation Study, Khanisyah Erza Gumilar, Grace Ariani, Priangga A. Wiratama, Rimbun Rimbun, Tri H. Yuliawati, Hong Chen, Ibrahim H. Ibrahim

BioMedicine

Background: Integrating artificial intelligence-based large language models (AI-LLMs) into medical and other scientific domains is increasingly recognized as a tool to support complex tasks, such as interpreting histopathology slides and scientific figures. AI-LLMs can simplify these processes by providing clearer explanations. By improving accessibility and comprehension, AI-LLMs can significantly assist healthcare professionals in diagnosing and therapy determination. Students and the public also find it easier to understand complex scientific concepts and images.

Objectives: This study explores the capability of AI-LLMs in interpreting histopathological slides and scientific images. This study aims to evaluate the performance of AI-LLMs in supporting diagnostics and …

Decyl Caffeate Inhibits The Proliferation Of Human Triple-Negative Breast Cancer Cells, Che-Yi Chao, Woei-Cheang Shyu, Chih-Lung Lin, En-Pei Isabel Chiang, Yueh-Hsiung Kuo, Feng-Yao Tang

BioMedicine

Background: Over recent decades, considerable attention has been directed toward the discovery of novel compounds capable of targeting survival-related signaling networks as therapeutic candidates for triple-negative breast cancer (TNBC). Central to TNBC pathobiology are the Akt/mTOR and MAPK/ERK signaling axes, both contribute to tumor progression and therapeutic resistance. Caffeic acid (CA), a naturally derived phenolic compound with anti-inflammatory activity, has previously been investigated for its anti-cancer potential. Purpose: In the present study, we explored the therapeutic value of newly synthesized CA derivatives in TNBC models using both cellular and animal-based systems. Methods: The anti-tumor efficacy of these CA derivatives was …

The Association Between The Gnb3 Rs5443 C/C Genotype And Obesity Phenotypes In Taiwanese Individuals, Ling-Yi Xiao, Zi-Lun Lai, Yang-Di Su, Szu-Yun Wang, Nia-Jia Zheng, Po- Ren Hsueh

BioMedicine

Background: The prevalence of obesity has increased significantly over the

years, and its health concerns cannot be underestimated. Obesity not only

causes potential mobility limitations in daily life but also increases the risk of

developing cardiovascular diseases, diabetes, cancer and other health

conditions. While an imbalanced diet and lack of exercise are well-known

causes of obesity, genetic patterns also influence its development. Although the GNB3 gene is known to be involved in lipid metabolism and fat cell

differentiation, studies have shown inconsistent associations between a

common single nucleotide polymorphism of GNB3 (c.825C>T, rs5443) and

obesity across different populations. Therefore, …

Chinese Herbal Medicine Treatment And The Association With Long-Term Major Adverse Cardiac Events In Patients With Chronic Kidney Disease: A Propensity-Score Matched Cohort Study, Yuan-Ching Liao, Mei-Yao Wu, Cheng-Li Lin, Chiz-Tzung Chang, Peter Mayer, Hung-Rong Yen

BioMedicine

Background: Cardiovascular disease (CVD) represents the leading cause of

mortality among idividuals with chronic kidney disease (CKD). While Chinese herbal

medicine (CHM) is commonly used by CKD patients in Taiwan, the impact of CHM

use on cardiovascular outcomes in this population remains insufficiently understood.

Aims: This study aimed to evaluate the association between CHM use and the long-

term risk of major adverse cardiac events (MACEs) in patients with CKD.

Methods: Data were obtained from the Taiwan National Health Insurance Research Database to identify patients aged over 20 years with newly diagnosed CKD. A 1:1 propensity score matching was performed …

Increased Dairy Product Consumption Is Associated With Shorter Telomere Length In Buccal Cells Among Normotensive Adults, Hsin-Hwa Tsai, Yang-Di Su, Zi-Lun Lai, Chia-Yu Lin, Shu-Fan Lin, Hsiu-Ching Hsu, Wen-Yuan Lin, Po-Ren Hsueh

BioMedicine

Background: Telomere length (TL) is a biomarker of biological aging and a predictor of age-related diseases. Dietary patterns, including dairy consumption, may influence telomere dynamics, but the evidence remains limited, particularly in Asian populations. This study investigates the association between dairy consumption and relative telomere length (RTL) in Taiwanese adults, with results analyzed by hypertension status.

Methods: A cross-sectional survey was conducted among 259 adults in Taipei, Taiwan. RTL was measured using quantitative PCR from buccal cells. Dietary intake was assessed through self-reported questionnaires, with a focus on dairy frequency and fat content. Multiple linear regression models were used to …

Temporal Induction Of The Homeodomain Transcription Factor Nkx2-1 Is Sufficient To Respecify Foregut And Hindgut Endoderm To A Pulmonary Fate In Xenopus Laevis, Brian Hyatt, Erin Lundberg, Rachael Eye, Scott Rankin, Aaron M. Zorn

Day of Scholarship

During development the expression of numerous genes is required to direct the proper formation of cells, tissues and organs. One special class of genes, transcription factors, code for proteins which regulate the expression of multiple genes to ensure tissues and organs develop at the right time and place. One transcription factor, NKX2-1, is necessary for proper lung formation in vertebrates. We investigated if NKX2-1, on its own, could drive the formation of lung tissue in the frog, Xenopus laevis. Both frog and human NKX2-1 was able to cause the formation of pulmonary tissue, examined by the expression of lung specific …

Controlled Epidemics: Examining The Effect Of Virus Density And Strain Competition In Gulf Fritillary (Dione Vanillae), Lizbeth Reyes

Biology: Student Scholarship & Creative Works

This study examines how different virus strains compete within a single host population of Dione vanillae butterflies, which is important in the study of disease ecology research. For this study, we conducted controlled experimental epidemics on passionflower plants (Passiflora caerulea) in the field. First, we infected caterpillars with one of two different virus strains from Butterfly Farms in North County (BFF004) and the City of San Diego (GRM060) and placed these caterpillars on P. caerulea plants in one of three treatments (control, BFF004, and GRM060; n=3 plants per treatment). Next, we allowed healthy butterflies in the local population to lay …

Effectiveness Of A Network Open House Model To Recruit Trainees To Post-Baccalaureate Stem Programs, Sarah Mordan-Mccombs, Scott Takeo Aoki, Lindsay Lewellyn, Sarah Justice, Neetu Tewari, Jorge Cantu, Robert Seiser, Ahmed Lakhani, Jennifer R. Kowalski

Biology Faculty publications

Post-baccalaureate (post-bac) programs can be instrumental in strengthening science training and expanding STEM career opportunities for junior trainees. Many of these sponsored programs are designed to increase research exposure for underrepresented minorities, including African American, Hispanic, Native American, and first-generation college students, among others. Recruiting trainees to post-bac programs can be challenging for reasons including a lack of awareness about available programs. To address this gap, an Open House event was created with the goal of raising awareness and generating interest among potential students for future post-bac programs. Students were recruited from partnering Minority Serving Institutions (MSIs) to attend a …

Social Roles And Emotional Distress Are Associated With Successful Aging In Adult Childhood Cancer Survivors, Samah Hayek, Kimberly A Miller, Michael E Roth, Michelle A T Hildebrandt, David R Freyer, Joel Milam

Faculty, Staff and Student Publications

Childhood cancer survivors (CCS) face complex long-term health consequences resulting from intensive treatments that may impact their aging trajectory. This study assessed the associations between social role attainment, emotional distress, and indicators of successful aging (SA) among adult CCS, considering treatment intensity. SA, is a multidimensional concept encompassing physical, cognitive, and psychosocial well-being, provides a framework for understanding and optimizing outcomes in this population. This cross-sectional study utilized data from Project Forward, a cohort, of 1,248 adult CCS diagnosed between 1996 and 2010. Participants were identified through the Los Angeles County Cancer Surveillance Program and completed a comprehensive self-report survey …

Cover And Contents

Turkish Journal of Veterinary & Animal Sciences

No abstract provided.

Comparison Of The Mars And Xgboost Algorithms For Predicting Body Weight In Kalahari Red Goats, Louis Thobela Tyasi, Cem Tirink, Kwena Mokoena, Hasan Önder, Uğur Şen, Demet Çanga Boğa, Tolga Tolun, İsmai̇l Gök, Yüksel Aksoy, Lütfi̇ Bayyurt, Ömer Faruk Yilmaz

Turkish Journal of Veterinary & Animal Sciences

Estimating goat body weight from morphometric traits is essential for growth monitoring, health management, and welfare. Accurate weight informs nutrition, clinical interventions, and mating decisions. In this study, the MARS and XGBoost algorithms were used to estimate the live weight of Kalahari Red goats based on body measurements. A dataset containing body length (BL), birth type (single, twin, or triplet), withers height (WH), rump height (RH), and heart girth (HG) information for 200 goats was used in the training and testing of the models. The performances of the models were evaluated with the goodness-of-fit criteria. XGBoost showed a performance of …

Hatchability And Nestling Viability In An Extremely Short-Beaked Pigeon Breed, Türker Savaş, Hakan Erdem, Cemi̇l Tölü

Turkish Journal of Veterinary & Animal Sciences

The selection of hobby animals for breeding by enthusiasts is often based on aesthetic considerations, which can have a negative impact on the animals’ biology. Furthermore, “extreme traits” that deviate from ancestral norms, such as short beaks in pigeons, can be detrimental to the animal’s welfare. Beaks perform many essential functions, such as facilitating feed intake and eggshell breaking. The present study investigates possible hatching problems and the nestling viability of short-beak pigeons through a study of the breeding records of Mısıri (short beak), Baska (medium beak), and Turkish Dönek (normal beak) pigeons, as well as data from one breeding …

Determination Of Cortisol Concentrations Using Different Biomarkers In Dairy Goats During Pregnancy, Fatma Mi̇ra Güven, Erkan Pehli̇van

Turkish Journal of Veterinary & Animal Sciences

Pregnancy is considered one of the most physiologically stressful periods in mammals. In the present study, serum and hair cortisol concentrations were measured in nine female Akkeçi goats, including five primiparous (1.5 years old) and four multiparous (3.5 years old), to evaluate chronic stress during pregnancy. Hair samples were collected in October, November, and December following the introduction of bucks to the herd and within the first 12 h postpartum (corresponding to the 1st, 2nd, 3rd, and 7th sampling times). Hair samples were not collected in January, February, or March (the 4th, 5th, and 6th sampling times) due to insufficient …

Effects Of Zinc-Reinforced Clinoptilolite On Hematological Parameters, Igg Levels, And Enzyme Histochemistry In Fattening Lambs, İhsan Kisadere, Muhi̇tti̇n Zengi̇n, Özkan Demi̇rbaş, Mehmet Faruk Aydin

Turkish Journal of Veterinary & Animal Sciences

The study aimed to determine the effects of zinc-reinforced clinoptilolite supplementation to the diet on selected hematological parameters, serum immunoglobulin G (IgG) concentrations, and enzyme histochemical characteristics in fattening lambs. For this purpose, 40 healthy male lambs were randomly allocated into five equal groups: control (C; n = 8), natural clinoptilolite (NC; n = 8), zinc (ZN; n = 8), natural clinoptilolite plus zinc (NCZN; n = 8), and zinc-reinforced clinoptilolite (ZNRC; n = 8). All animals were housed in individual pens, and the supplementation period continued for 42 days. Blood samples were collected before the trial (day 0) and …

Zinc Imbalance And Its Effect On Nutritional Status, Behavior, Gut Microbiota, And Inflammatory Biomarkers In Btbr T+Itpr3tf/J And C57bl/6j Mice: Insights Into Autism Spectrum Disorder, Asma Nasrullah Ahmadani

Thesis/ Dissertation Defenses

Background: Autism spectrum disorder (ASD) has been associated with altered zinc homeostasis and disruptions in the gut microbiota. Zinc is a vital trace mineral involved in multiple biological processes such as inflammatory regulation, intestinal integrity, and neurobehavioral function, and has an important role in influencing microbial activity along the gut–brain axis. Both deficiency and excess of zinc can alter microbial balance. However, their collective effects on behavior, overall physiology, and mineral status in ASD-relevant models are not yet fully understood.

Aim: This comprehensive study explored the effect of dietary zinc variation on body weight, food intake, behavior, gut microbiota composition, …

Science Communication Tools: Rubrics For Generating Posters And Manuscripts That Are Authentic To The Practice Of Science, Quinn Vega

Department of Biology Faculty Scholarship and Creative Works

Traditional undergraduate science courses often prioritize content mastery over authentic engagement with the scientific process. Course-based research, also referred to as course-based undergraduate research experiences (CUREs), addresses this limitation by immersing students in authentic scientific practice. In course-based research, assessment practices can also mirror the authentic scientific practice, where extensive formative feedback supports refinement of skills and understanding. Here, we present two rubrics designed to support the teaching and assessment of science communication in a way that reflects how scientists prepare to disseminate their research findings. One rubric is for creating scientific posters and another for writing short-format manuscripts. Developed …

The Effect Of St. John’S Wort Solution On Climbing Activity Reflecting Depressive-Like Behavior In Drosophila Melanogaster To Model Seasonal Depression In Low Sunlight Exposure, Madison Simon

Journal of the South Carolina Academy of Science

Although depression is a known substantial threat to public health, seasonal affective disorder (SAD) is under-researched. SAD refers to recurrent seasonal patterns of depression. This depressive disorder has few accepted treatments. The purpose of this study was to test whether St. John’s Wort (SJW), a natural supplement with researched antidepressant properties, could be a cheaper alternative with fewer limitations for treating SAD. SJW’s efficacy as a seasonal depression inhibitor was tested through a Drosophila depression-like model using climbing activity to reflect depressive behavior. It was hypothesized that as the concentration of SJW solution increases, the climbing activity of stressed Drosophila …

An Examination Of Trends And Factors Influencing Teen Retention In 4-H Youth Development Program: A Case Study, Bethany R. Corona

LSU Master's Theses

Teen participation in 4-H has declined nationally in recent years, raising concerns about long-term program sustainability and youth engagement. Understanding the factors that influence teens’ decisions to remain involved in 4-H is critical for informing program design and delivery. Guided by the theory of planned behavior, this qualitative study explored teen perceptions of 4-H programming, youth voice, social influences, communication, and barriers to participation. Data were collected through two group interviews with teen 4-H members representing multiple parishes. Interviews were audio recorded, transcribed verbatim, and analyzed using the constant comparative method through a three-phase coding process consisting of open, axial, …

Limitations Of Genetic Testing, Tony N. Jelsma

Faculty Work Comprehensive List

"Knowing about a pathogenic variant may sharpen our awareness of our mortality, but it should not fundamentally change our perspective on life."

Posting about the advantages and disadvantages of genetic screening from In All Things, an online hub that offers insight into maintaining and faithful and orthodox Reformed Christian worldview while fearlessly engaging in every aspect of contemporary life – until all is made new.

Limitations of Genetic Testing

Science Communication Tools: Rubrics For Generating Posters And Manuscripts That Are Authentic To The Practice Of Science, Tamarah Lynne Adair, Swati Agrawal, Yesmi P. Ahumada-Santos, Regina Alvarez, Kirk Anders, Rebekah F. Hare

Biology Faculty Scholarship

Traditional undergraduate science courses often prioritize content mastery over authentic engagement with the scientific process. Course-based research, also referred to as course-based undergraduate research experiences (CUREs), addresses this limitation by immersing students in authentic scientific practice. In course-based research, assessment practices can also mirror the authentic scientific practice, where extensive formative feedback supports refinement of skills and understanding. Here, we present two rubrics designed to support the teaching and assessment of science communication in a way that reflects how scientists prepare to disseminate their research findings. One rubric is for creating scientific posters and another for writing short-format manuscripts. Developed …

Challenges, Stressors, And Resilience Resources Experienced By Older Black Women In Rural South Carolina Throughout The Covid-19 Pandemic, Arielle N'Diaye, Camryn Garrett, Sarah Wilcox Phd, Bankole Olatosi Ph.D., Xiaoming Li Ph.D., Shan Qiao Ph.D.

Faculty Publications

Background

Resilience has been established as a protective factor against mental health challenges (e.g., anxiety, depression). From an intersectional perspective, the stressors experienced by Black women living in rural communities are likely to have been exacerbated during the COVID-19 pandemic. This study aims to identify the challenges and stressors experienced and the resilience resources available to and relied upon by rural, Black women, during the pandemic.

Methods

Individual, in-depth, structured interviews (n = 24) were conducted among Black women living in rural South Carolina, recruited by local community health workers, from January to April of 2024. All interviews were recorded, …

Concordance Analysis Of Dna And Rna Profiling: The Md Anderson Impact2 Study In Precision Oncology, Stephanie T Schmidt, Mehmet A Baysal, Siqing Fu, David S Hong, Sarina A Piha-Paul, Aung Naing, Jordi Rodon Ahnert, Timothy A Yap, Ecaterina Elena Dumbrava, Jennifer Beck, Funda Meric-Bernstam, Apostolia Maria Tsimberidou

Faculty, Staff and Student Publications

DNA profiling is an established method for cancer treatment selection, while RNA profiling remains investigational. We explored associations between DNA and RNA alterations and between the number of genes with altered expression and overall survival (OS) using patient data from IMPACT2 (NCT02152254), a randomized study evaluating molecular profiling for guiding cancer therapy across tumor types. Molecular profiling, including DNA next-generation sequencing, was performed on all 829 patients in the IMPACT2 study. RNA profiling was performed by Tempus for 253 of 829 patients. We evaluated the concordance between DNA and RNA profiling, analyzed OS in 217 treated patients with …

Risk Stratification Of Low-Dose Cytarabine And Venetoclax In Patients With Aml Ineligible For Intensive Chemotherapy, Andrew H Wei, Panayiotis Panayiotidis, Pau Montesinos, Kamel Laribi, Vladimir Ivanov, Inho Kim, Jan Novak, Rebecca Champion, Walter Fiedler, Maria Pagoni, Julie Bergeron, Stephen B Ting, Jing-Zhou Hou, Takahiro Yamauchi, Jianxiang Wang, Stephen A Strickland, Michael R Savona, Tara L Lin, Anoop Enjeti, Ing Soo Tiong, Sangmin Lee, Gail J Roboz, Relja Popovic, Qi Jiang, Zihuan Liu, Yan Sun, Wellington Mendes, Brenda Chyla, Courtney D Dinardo

Faculty, Staff and Student Publications

Prognostic risk categorization aids treatment selection for patients with acute myeloid leukemia (AML). Although the European LeukemiaNet (ELN) classifications (2017 and 2022) for AML have been used to stratify outcomes for patients receiving intensive chemotherapy, their application to patients receiving less intensive therapy, such as azacitidine plus venetoclax, has been less satisfactory. In response, a 4-gene classifier that stratifies older patients with AML unfit for intensive chemotherapy into those with higher benefit (wild type), intermediate benefit (FLT3-internal tandem duplication [ITD] or NRAS/KRAS mutation), or lower benefit (TP53 mutation) after azacitidine plus venetoclax treatment was developed. We hypothesized that this 4-gene …