Digital Commons Network^™

Polylactic Acid (Pla) Microplastic Toxicity In C. Elegans, Maria Gamez, Dana Farley, Chiara Maldonado, David Mares

Posters - 2026

• Microplastics (MPs, > 5 mm1) are an environmental pollutant that pose health risks to humans. • Primary MPs are created for direct use or plastic stocks, and secondary MPs are created when plastics in the environment break down over time. • Microplastic exposure can cause oxidative stress, metabolic disorders, immune responses, neurotoxicity, and reproductive issues2-3. • Polylactic Acid (PLA) is a biodegradable plastic used in everyday items, such as food packaging, and as a polymer in 3D printing. • PLA exposure to fish causes organ damage5 and C. elegans exposure to primary PLA MPs and UV-aged MPs reduced reproduction6-7 . …

Muscular Degeneration In Caenorhabditis Elegans By Microplastic And Chemical Exposure Through Locomotion Analysis, Juan Pablo Olvera Rodriguez, Angelica Cue-Leon, David Mares

Posters - 2026

We use Caenorhabditis elegans (C. elegans) as a model organism to investigate the effects of microplastic and chemical exposure on muscle structure and function.

• Microplastics (MP) are pieces of plastic less than 5 μm, and Nanoplastics (NP) are less than 1 μm. Microplastics are man-made pollutants and have been found in the environment and in the food and water sources, including drinks from plastic bottles (Hu, 2020). • Our work relates environmental contamination to potential biological consequences at the muscular level, providing insight into how pollutants may contribute to degenerative damage.

• Polystyrene (PS) MPs alone and combined with …

Analyzing The Effect Of Melatonin On B Cells' Expression Of Cd36, Caitlyn Pinaga, Gabriella Galdeano, Nayi Gonzalez, Emma Welsch

Posters - 2026

• B cells specifically target pathogens and proliferate to make a plasma cell and secrete antibodies when stimulated. They use glucose as a main energy source but can also use fatty acids. • CD36 is a glycoprotein involved in the transportation and metabolism of fatty acids. • B cells express CD36 in unusually high levels in autoimmune patients. It is unknown as to how this occurs. • Melatonin is a hormone produced in the pineal gland, commonly known for its function in regulating the circadian rhythm. • Melatonin is also known as a master cell regulator and is believed to …

Bird Population Response To Abiotic And Biotic Stresses In Urban & Restored Oak Savannah Habitat, Annabelle Ball

Posters - 2026

Birds are heavily impacted by urban development. Many experience habitat fragmentation and removal of food sources. However, other species can adapt to and even thrive in urban environments, thanks to certain tree species, bird feeders, and buildings. In North America, the house sparrow and the European starling are invasive species that benefit from urban environments (Bartelt, 2011; Fitzwater, 2014). Meanwhile, many habitats across the continent are experiencing restoration/rewilding efforts for the benefit of birds. One such habitat is the oak-savannah, which is characterized by 10-30% canopy cover and a mixture of prairie & woodland vegetation, supporting a high diversity of …

"I Need Help!": Can Ashwagandha Reduce Inflammation-Driven Oxidative Stress Without Inducing Cytotoxicity In Macrophages?, Caitlyn Pinaga

Posters - 2026

Ashwagandha has been shown to reduce inflammation in humans by lowering pro-inflammatory cytokines and oxidative stress markers, helping regulate immune responses. It is commonly used to support conditions linked to chronic inflammation, such as stress-related disorders, arthritis, and metabolic imbalance. THP-1 macrophages are a part of the body's immune response to inflammation. While they produce a lot of different responses to inflammation, one of them is the production of Reactive Oxygen Species (ROS). N-acetylcysteine (NAC) is established to be a powerful antioxidant and is used as a positive control. My study aims to understand how ashwagandha, a popular supplement, affects …

Saved From The Brink Of Collapse: Evaluating The Success Of The Sussex Kelp Recovery Project, Keagan Farrell, Kelly Swyers, Adan Vasquez

Posters - 2026

Kelp forests are vital to ocean health, they provide habitat, food, and nursery grounds for a variety of marine species. These forests support fisheries, improve water quality, and help to protect coastlines (Eger et al. 2024). The Sussex Kelp Recovery Project is a marine conservation effort aimed at restoring their kelp forests along the Sussex coastline in southern England. This area lost up to 96% of its forests mainly due to the fishing practice trawling. Trawling is used to gather large amounts of fish, while dragging a net at the seafloor (Our history 2026). The main goal of the Sussex …

The Effects Human Presence Has On Dolphins Within A Natural Lagoon Enclosure In Managed Care, Mario Martinez, Winter Saldaña

Posters - 2026

● Previous research looked into the overall behavior of dolphins in managed care using observations of location (Dudzinski & Hill, 2025).

● This study provides opportunities to investigate the effects of human presence on dolphin activity and space use (Lauderdale et al., 2022; Trone et al., 2005).

● Purpose - To determine if human presence affects dolphin behavior / spatial use of natural lagoon.

● H1: Dolphin clustering will be seen in the most Northern part of the lagoon and area associated with humans consistently (Melillo et al., 2009).

● H2: Higher activity levels will be observed during the “during” …

Exploring Base Excision Repair Using Gene Knockout, Gregory Guantos, Cailyn Brock, Kamily Visser, Dylan Vargas

Posters - 2026

•             Base Excision Repair (BER) fixes damaged 3DNA bases throughout the cell cycle by removing damaged bases and replacing either one nucleotide in short-patch BER or a short stretch of nucleotides in long-patch BER. (Hindi, 2021, Cellular and Molecular Life Sciences:CMLS)

•             4G-quadruplexes (G4s) are 4 stranded secondary DNA structures formed in guanine rich areas of DNA and RNA. (Rhodes, 2015, Nucleic Acids Research)

The URA3 plasmid was used as a PCR template to make a gene deletion construct, then yeast were transformed so APN1 was replaced by URA3. 2APN1 encodes a major DNA repair enzyme in yeast, and strains …

Dietary Influence And Neurodegenerative Disease Risk: Insight From Alzheimer’S And Parkinson’S Disease, Madison Wirt

Honors Projects

As populations age, neurodegenerative diseases (NDD) become significantly more prevalent and impactful on patients and families. Clinical research has suggested a correlation between diet, gut health, neuroinflammation and mitochondrial dysfunction to the onset and development of specific and severe NDDs, especially Alzheimer’s disease (AD) and Parkinson’s disease (PD). This research focuses on the relationship behind the gut-brain axis, micronutrient status and how diet influences the development of both AD and PD. Ten randomized, controlled trials were analyzed from the literature to investigate the impact of these factors on the inflammatory, metabolic and antioxidant processes. The studies focused on the use …

Characterising Soil Microbial Communities In Thinned And Unthinned Eucalyptus Forest In Southwestern Australia, Aaron J. Brace, Laurence Dugal, Josephine Hyde, Kristen Fernandes, Anna J.M. Hopkins, Emma Stevens, Gavan S. Mcgrath, Katinka X. Ruthrof

Research outputs 2022 to 2026

Climate change stress in forested ecosystems globally has led to die-off events, and management actions are needed to help mitigate these pressures. Forest thinning has been proposed to reduce competition for moisture between trees, however, the effects of forest thinning on lesser-studied organisms, such as soil microbial communities, need quantification. Soil microbial communities are essential for processes such as nutrient cycling and can be used as indicators of below-ground health and as an overall proxy for biodiversity. This study examined soil microbial communities, and site and soil characteristics, directly at the base of trees and in inter-tree spaces in a …

Exchanging Genes Within A City: Analysis Of Pollen Flow Patterns In A Narrow Endemic Plant Species Threatened By Urbanisation, Nicola Delnevo, Andrea Piotti, Eddie J. Van Etten, William D. Stock, David L. Field, Margaret Byrne

Research outputs 2022 to 2026

Human-driven habitat fragmentation is known to disrupt gene flow in a wide range of species. However, its consequences for endemic plant species that depend on specialised invertebrate pollinators remain poorly understood. Although honeybees are capable of travelling considerable distances while foraging, many small-bodied native bee species lack the capacity to expand their foraging ranges or cross anthropogenic barriers, rendering habitat fragmentation the most significant threat to these ecological interactions. In many long-lived plants, the time since fragmentation has not been long enough for detectable changes in the genetic composition of standing populations. Changes in dispersal patterns observed in seedlings may …

Exploratory Evaluation Of Handler Performance And Processing-Related Outcomes During Cattle Handling, Morgan Marquardt

Department of Animal Science: Dissertations, Theses, and Student Research

Animals have several critical roles in society, including the production of food and fiber, and companionship. Contact with animals has been shown to benefit human physical and mental health, yet little research has investigated the impact that humans have on animals. Handler skill may considerably affect the extent to which humans influence animal health and wellbeing, and this may be a critical consideration for animal agriculture. Objective assessment of cattle handler skill has received limited attention in the scientific literature, despite its importance to human safety and animal welfare. When compared to less skilled handlers, highly skilled cattle handlers may …

Oral Multienzyme Supplementation Alters Postprandial Plasma Nutrient Concentrations After A Mixed Meal In Healthy Middle-Aged And Older Adults: A Randomized, Double-Blind, Placebo-Controlled, Crossover Trial, Max T. Deutz, Andrew T. Askow, Sean M. Garvey, David A. Alvarado, Takeshi M. Barnes, Zan Zupancic, Alexander V. Ulanov, Jared W. Willard, Hannah D. Holscher, Brett R. Loman, Nicholas A. Burd

Kinesiology & Sport Management Scholarship

Background: Age-related decline in digestive function increases malnutrition risk. Supplementing meals with digestive enzymes may improve macronutrient digestion and bioavailability in adults reaching older ages.

Objectives: To assess postprandial plasma nutrient concentrations after co-ingestion of a mixed meal and a mixture of 6 enzyme preparations (ENZ), including proteases, lipase, amylase, and glucoamylase.

Methods: Thirty middle-aged and older adults (56 ± 11 y; 18 females, 12 males) ingested chicken, peas, potatoes, and butter (435 kcal; 34 g protein, 51 g carbohydrate, 11 g fat) with either ENZ or placebo (PLA) in a randomized crossover fashion. Blood samples were collected …

Fisheries Research Report No. 358 - Recreational Fishing For Blue Swimmer Crab In Cockburn Sound: Estimates Of Effort And Catch In 2024/25, Alissa C. Tate, Claire B. Smallwood, Eva K M Lai

Fisheries Research Reports

The Cockburn Sound Blue Swimmer Crab (Portunus armatus) fishery was reopened to recreational fishing in December 2024 following a 10-year closure. Monitoring was essential to assess fishing effort and catch for this species.

A boat ramp (bus route) survey at four key access points (Leeuwin, Woodman Point, Palm Beach, and Point Peron) and shore-based roving creel survey (Fremantle South Mole to Point Peron) were used to collect data from December 2024 to May 2025. These surveys provide estimates of fishing effort, total catch (kept and released), harvest and catch rates for Blue Swimmer Crab in Cockburn Sound.

A total of …

Investigating The Missense Mutation In Men1 At Amino Acid Position 331 Changing Asparagine To Isoleucine, Anika Prasad

Expo Student Presentations

2026 Spring Expo Online Poster Presentation

• Biological & Life Sciences

An Insight Into The Venom Gland Proteome Of The Two-Spined Trap-Jaw Ant, Odontomachus Haematodus, Aaron Morgan

Honors Theses

Ants are ecologically important and highly abundant organisms, playing key roles in many ecosystems. One recently introduced species in the southeastern United States is Odontomachus haematodus (the two-spined trap-jaw ant), a South American native that has often been misidentified, contributing to limited research on its biology and venom composition. This study aimed to provide the first detailed characterization of the venom apparatus and proteome of O. haematodus. Specimens were collected and dissected, and venom-related proteins were extracted and analyzed using SDS-PAGE and tandem mass spectrometry (MS/MS-ESI). Confocal microscopy was also used to visualize the sting apparatus, including F-actin filaments and …

Characterization Of A Core Fungal Community And Captivity-Induced Gut “Mycobiome” Change In Fowler's Toad (Anaxyrus Fowleri), Alexander J. Bradshaw, Sinlan Poo, Tracey E. Malter, Rylie M. Strasbaugh, Brianna Bodner, Madison R. Hincher, Anne Devan-Song, Javier F. Tabima

Biology

Amphibious animals, such as frogs, are found at the intersection of aquatic and terrestrial ecosystems. They may serve as keystone and sentinel species and play key roles in nutrient cycling and food webs. In recent decades, amphibians have experienced drastic population declines due to habitat loss, climate change, and disease. These declines have prompted investments in ex situ conservation and captive breeding programs, which aim to reduce extinction risk by creating assurance colonies and reintroducing individuals once threats are mitigated. A critical component of these programs is proper husbandry, which ensures the health and longevity of captive populations and their …

Epigenetic Mechanisms For Mediating The Transmission Of Prenatal Maternal Stress And Associated Neurodevelopmental Outcomes, Kaylee Vis

Senior Honors Theses

The hypothalamic-pituitary-adrenal (HPA) axis is the primary stress response system responsible for regulating cortisol release. During pregnancy, maternal stress can dysregulate the HPA axis, leading to elevated cortisol and corticosterone levels that impact fetal development. Excessive prenatal exposure to stress has been associated with alterations in fetal brain structure and function, increasing the risk for neurodevelopmental disorders such as autism spectrum disorder, attention-deficit/hyperactivity disorder, and anxiety. Emerging research supports the idea that these neurodevelopmental effects may be transmitted through epigenetic mechanisms. However, a gap remains in the literature regarding the specific mechanism of interaction between environmental stressors, heritable epigenetic information, …

Sox18 Unleashed Exploring The Transcriptomic Landscape - Differential Gene Expression Analysis Via Rna Seq In Overexpressed Sox18, Van Nguyen, Kahlie Hernandez, Monabelle Elbayeh

Posters - 2026

Cancer remains a leading cause of death worldwide, and childhood sarcomas such as Rhabdomyosarcoma (RMS) and Ewing Sarcoma (ES) are particularly aggressive with limited targeted treatment options. Despite advancements in cancer therapies, metastatic sarcomas still have a survival rate below 30%, emphasizing the need for new therapeutic targets. SOX18, a transcription factor has played a role in vascular development and endothelial differentiation, functioning as a key driver of angiogenesis. In cancer, increased SOX18 expression has been linked to dysregulated cell migration, invasion, and therapy resistance mechanisms. However, the extent to which SOX18 influences RMS and ES at the transcriptional level …

Detecting Cancer Genes Using Graph Neural Networks, Marvin Masabo Nkaka

Posters - 2026

• Cancer survival prediction is challenging due to the complexity of genomic data and limited samples especially for rarer cancer types. • To address this challenge, we developed an Artificial Neural Network (ANN) model for survival analysis using RNA-sequencing gene expression data from The Cancer Genome Atlas (TCGA). • Moreover, a key concept we investigate was how transfer learning enhanced our model’s performance especially for rarer cancer types difficult to perform accurate survival analysis due to their limited samples.

Developing 'A Guide To Agricultural Education Advisory Committees: Development, Uses, And Transitions', Christine Schuler

Department of Agricultural Leadership, Education, and Communication: Dissertations, Theses, and Student Research

This project developed a practical guide to support agricultural education advisory committees in their development, use, and long-term sustainability. Strong school-community partnerships are essential to effective agricultural education programs, particularly in small, rural communities where programs serve as connections between the school and local industry. Advisory committees play a critical role in maintaining these relationships, yet many agricultural educators lack clear, accessible resources to guide this work. This project addressed that need by creating a comprehensive, practice-based guide established from research and professional experience.

This guide started from a review of literature on school-community partnerships, advisory committees, and agricultural education …

Factors Influencing Sex-Based Movement Patterns In The Banded Garden Spider, Argiope Trifasciata (Forskal, 1776), Brandon R. Marrone

School of Biological Sciences: Dissertations, Theses, and Student Research

Movement is a fundamental ecological process shaping how individuals acquire mates, secure resources, and navigate heterogeneous landscapes. In species with extreme sexual size dimorphism (eSSD), such as the orb‑weaving spider Argiope trifasciata, males and females are predicted to experience different movement pressures driven by their reproductive roles. This thesis examines how individual traits and environmental conditions jointly influence movement across both sexes. Across two field seasons, we used intensive mark–recapture methods to quantify natural movement patterns of males and females within mapped prairie plots. The first chapter tested long‑standing predictions gravity hypothesis, which posits that smaller males benefit from …

First High-Quality Genome Assembly Of Umbelopsis Nana Isolated From Forest Soil, Ryuka Iizuka, Tomohiro Suzuki, Nozomu Watanabe, Javier F. Tabima, David Hibbett, Yoko Katayama, Makoto Yoshida

Biology

Species of the genus Umbelopsis are ubiquitous soil saprotrophic fungi known for their role in ecological nutrient cycling and biotechnological potential in lipid production. Here, we present the genome assembly of Umbelopsis sp. strain THIF13, which is characterized by its capability to produce carbonyl sulfide. Using PacBio Sequel long-read data, a high-quality genome assembly was generated comprising 18 contigs totaling 27.2 Mb, which is larger than other Umbelopsis genomes, with an N50 of 1.77 Mb. Notably, 16 contigs were flanked by telomeric repeats at both ends. The assembly showed high completeness, with 98.1% complete Benchmarking Universal Single-Copy Orthologs and 11.15% …

Alabama Prairie Reconstruction: Comparing Management Pathways For A Southeastern Landscape, Cecelia R. Geyer

Theses

Southeastern prairies are highly biodiverse yet increasingly threatened by land-use change, fire suppression, and habitat fragmentation. These disturbance-dependent systems rely on periodic fire and grazing to maintain plant diversity and ecosystem function. This study evaluates prairie restoration strategies by testing predictions from the Intermediate Disturbance Hypothesis and the Patch Mosaic Burn Hypothesis. The study site was divided into three treatments: prescribed burned only (B), prescribed burned with mowing (BM) to simulate grazing, and an undisturbed control (C). A standardized mix of native plant seeds was broadcast across all treatments. Ecosystem health and function were evaluated using plant community diversity and …

Perceived Barriers To Integrated Crop–Livestock Systems: Evidence From A Nebraska Producer Survey, Omid Tarashtwal

Department of Agricultural Economics: Dissertations, Theses, and Student Research

Integrated crop-livestock systems offer agronomic, economic, and environmental benefits, yet reintegration has been slow across the Northern Great Plains despite sustained interest. Existing research has documented barriers to transition conceptually, but quantitative evidence on which barriers producers perceive as most important and which producer and operation characteristics are associated with stronger barrier perceptions remains limited. This thesis addresses that gap using data from the 2023 Nebraska Agriculture Operator Survey.

The analysis focuses on 241 Nebraska producers, including 179 crop-only and 62 livestock-only operations. Seven models are estimated, combining ordered logit estimation for single-item barriers with linear regression for averaged barrier …

Mixed Methods Evaluation Of A Shipped Medically Tailored Meal Pilot Intervention, Shelly Palmer, Laura E. Balis, Amy L. Yaroch

Journal of Patient-Centered Research and Reviews

Purpose: Medically tailored meal (MTM) programs generally demonstrate meaningful health outcomes for patients. There is a need to evaluate novel MTM delivery methods to understand participant and organizational-level impacts. The objectives of this study were to assess a shipped MTM pilot intervention, including 1) initial and ongoing client reach, 2) effects on clients’ health and behaviors, and 3) staff members’ perceptions of factors that influence intervention maintenance.

Methods: Based on the reach, effectiveness, adoption, implementation, maintenance (RE-AIM) framework, the mixed methods design included quantitative data collected from participants and qualitative data collected from MTM provider staff. The pilot took place …

Do Young Belugas Play More Or Less Than Adult Belugas?, Winter Saldana

Posters - 2026

★ The presence of play is used as an indicator of well-being in many species including belugas (Hill et al., 2017; Hill & Ramirez, 2014). ★ Adult and young belugas engage in similar types of play, but the frequency with which belugas play with objects, the actions used during those object play bouts, and the type of materials the objects consist of have not been examined in detail (Hill & Ramirez, 2014). ★ The purpose of this study was to explore if object play exhibited by belugas varied in frequency of play bouts, actions, and materials used by young and …

Evaluation Of Prosthetic And Orthotic Technologies Using Ai Technology, Iris Reyna, Samerial Brown, Gary Guerra, Ed Khu

Posters - 2026

Individuals with lower limb prosthetics have amputations and abnormal limb motions, which poses a high risk of injury when doing everyday activities. In this field, evaluation of individuals' prosthetics are done through biomechanical analysis and gait analysis. This analysis is used to study human movement and evaluate the mobility and quality of life of amputee patients as well as determining the modifications, positioning, and alignment needed to ensure stability with prosthetic and orthotic technologies (Kumar & Bhowmik, 2024). Trunk flexion has importance to the study because of its impact on lower limb kinetics, kinematics, the increase of ground reaction forces, …

The Effects Of Cannabis Use On Physical Activity, Nitya Chiru, Brionna L. Smith, Karen L. Cropsey, Elizabeth K. Lee

Expo Student Presentations

2026 Spring Expo Poster Presentation

• Biological & Life Sciences

Improving Cannabis Sativa's Phytoremediation Capabilities Of Forever Chemicals Through Microbial Inoculations, Tyler S. Jolin

All NMU Master's Theses

Per- and polyfluoroalkyl substances (PFAS) are persistent environmental contaminants that pose ecological and human health risks due to their resistance to degradation and potential for bioaccumulation. Phytoremediation has emerged as a potential strategy for mitigating PFAS contamination, and plant-microbe interactions may influence contaminant uptake and transport within plant systems. These studies investigated microbial inoculation methods and examined whether Bacillus subtilis could influence the uptake and distribution of perfluorooctane sulfonate (PFOS) in hydroponically grown hemp (Cannabis sativa L.). Plant height and stem diameter growth rates and biomass accumulation were compared across treatments with and without PFOS exposure and microbial inoculation. PFOS …