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An Examination Of Lysine Acetylation In The Context Of Bacterial Metabolism, Translation, And Regulation Of Gene Expression, Thomas Patrick Bank

Dissertations

Post-translational modifications are a critical tool bacteria employ to survive the challenges of microbial life. Acetylation continues to gain more recognition as a modification of unique importance because the sources of acetylation – acetyl-phosphate and acetyl-coenzyme-A – are critical metabolites associated with central carbon metabolism, acetate overflow metabolism and acetate assimilation. This suggests that acetylation, indelibly coupled to metabolic flux, integrates metabolic sensing with any cellular process or pathway where an acetylation alters the function of a protein. Intuitively, this makes sense. Of course, a bacterial cell would coordinate any cellular process closely with the amount of energy available to …

Animal Taxa Threatened By Adverse Fire Regimes In Western Australia: A Synthesis And Outlook, Tim S. Doherty, Mark G. Allen, Karlene Bain, Stephen Beatty, Allan H. Burbidge, Renee A. Catullo, Sarah Comer, Ben Corey, Saul J. Cowen, Robert A. Davis, Martin A. Dziminski, J. Anthony Friend, Bruce Greatwich, Mark S. Harvey, Hannah Killian, Michael E. Klunzinger, Damian C. Lettoof, Nicola J. Mitchell, Melinda Moir, Harry A. Moore, David L. Morgan, Ian J. Radford, Michael J. Rix, Tom Vigilante, Adrian F. Wayne, Corey Whisson, Nicole Willers, Matthew R. Williams

Natural Resources Research Articles

Adverse fire regimes threaten biodiversity, potentially leading to population declines and increased extinction risk. Understanding how varying fire regimes affect threatened species is essential for effective ecosystem management, including in Western Australia where diverse ecosystems are exposed to wildfires, Indigenous burning, and prescribed fire. We compiled and synthesised data on the threat of adverse fire regimes to threatened animal taxa in Western Australia. Using a threat classification scheme, we ranked adverse fire regimes as having either no, low, medium, high, or unknown impact on each taxon. A total of 212 taxa were reviewed, 153 (72%) of which are considered fire-threatened: …

Defective Dna Damage Response Is A Targetable Therapeutic Vulnerability In Esr1 Mutant Breast Cancer, Sarah K Herzog, Jessica H Stevens, Guowei Gu, Sandra L Grimm, Kloma Cardoza, Hangqing Lin, Amanda R Beyer, David G Edwards, Derek Dustin, Harry J Yang, Nicole Liang, Ashfia F Khan, Tasneem Bawa-Khalfe, Daniel J Mcgrail, Shiaw-Yih Lin, Cristian Coarfa, Suzanne A W Fuqua

Faculty, Staff and Student Publications

ESR1 mutations are the leading cause of endocrine therapy resistance and progression in ER-positive metastatic breast cancer. ESR1 mutations are detected in ~50% of metastatic breast cancer patients, and identification of effective targeted therapeutics are critically needed. Here, we identified enrichment of dysregulated replication stress and DNA damage responses in multiple ESR1 mutant models. Targeting the replication stress response utilizing checkpoint inhibition in combination with PARP inhibition synergistically suppressed growth, induced cell cycle arrest, and attenuated DNA replication. PARP inhibition blocked metastatic dissemination in vivo and reduced both PARP1 and ER-regulated protein expression. PARP trapping by olaparib treatment with or …

N-Palmitoyl Glutamine Is A Candidate Mediator Of Cardiorespiratory Fitness, Jeremy M Robbins, Mark Benson, Anthony R P Verkerke, Gaurav Tiwari, Shuliang Deng, Prashant Rao, Usman A Tahir, Julian Avila-Pacheco, Xu Shi, Yuntian Guan, Foje-Geh Tendoh, Jacob L Barber, Patricia E Miller, Andrew S Perry, Michael E Hall, Alexis C Wood, Kent D Taylor, Wendy S Post, Stephen S Rich, Matthew Nayor, James G Wilson, Gregory D Lewis, Ravi V Shah, Jerome I Rotter, Scott A Summers, Laura M Raffield, Shingo Kajimura, Claude Bouchard, Clary B Clish, Mark A Sarzynski, Robert E Gerszten

Children’s Nutrition Research Center Staff Publications

Background: Cardiorespiratory fitness is an integrative measure of cardiometabolic health and predictor of survival, yet little is known about its molecular underpinnings. Small molecule metabolites and lipids are increasingly recognized as exercise-stimulated signaling molecules and candidate molecular transducers of cardiorespiratory fitness.

Methods: We performed nontargeted liquid chromatography mass spectrometry-based plasma metabolomics in 654 participants (mean age, 35 years; 55% women) from the HERITAGE Family Study (Health, Risk Factors, Exercise Training, and Genetics) who had cardiorespiratory fitness (maximal oxygen uptake [VO2max]) measured by cardiopulmonary exercise testing and underwent 20 weeks of supervised endurance training. Metabolite-VO2max relationships were assessed using linear regression …

Sheep Umbilical Cord Mesenchymal Stem Cells: Biological Characteristics, Ethical Considerations, And Translational Applications, Hasan M. Agha, Mohammed A. Abdalqader, Oh Chin Kin Michelle, Sabaa M. Agha, Jantson Hoh, Abdulmutalib Alabeed Allaq, Amjad Abdulhadi Mohammed, Muneer Mohammed Saleh Alsayadi

AUIQ Complementary Biological System

Mesenchymal stem cells (MSCs) are widely used in regenerative medicine due to their self-renewal capacity, multilineage differentiation potential, and paracrine activity. Limitations associated with the sourcing and scalability of human MSCs have increased interest in xenogeneic alternatives, particularly sheep umbilical cord–derived MSCs (sUC-MSCs). These cells display high proliferative capacity, stable karyotypes, and a typical MSC immunophenotype (CD44, CD73, CD90, CD105), with low basal immunogenicity. sUC-MSCs exhibit osteogenic, chondrogenic, and adipogenic differentiation potential and secrete angiogenic and immunomodulatory factors, including VEGF, HGF, IGF-1, TGF-β, and IL-10. Owing to their biological similarities to human MSCs and accessibility from perinatal tissue, …

Capacity-Building Project To Progress The Implementation Of International Instruments To Combat Iuu Fishing (Capfish) : Summer Academy: West Africa, World Maritime University, Korea Maritime Institute, Ministry Of Oceans And Fisheries

Reports

The CAPFISH Project was created in response to an identified need for capacity-building to address illegal, unreported and unregulated (IUU) fishing and related matters - a complex problem affecting aquatic ecosystems, coastal communities, fishers and fish workers, and economic and national security. Over the last five years, the CAPFISH Project has provided training and educational materials on IUU fishing to developing country administrations around the world, most recently with a two-week regional Summer Academy for West Africa. This Academy ran for two weeks and included delegates from fisheries, maritime and enforcement agencies, industry, maritime training institutions, regional fisheries bodies, and …

The Gendered Heart: Closing The Gender Gap In Heart Disease Diagnosis And Treatment, Aiden Varghese

UROP Posters

Cardiovascular disease (CVD) continues to be the primary cause of death for both men and women in the United States, being characterized and treated in a historically male-centric manner. This research paper will address the disparities in risk factors for heart disease between genders, gender differences in diagnosis, and gender differences in management/treatment, thereby demonstrating the critical importance of incorporating sex specific factors into the field of cardiology. It has been demonstrated that lifestyle changes such as physical activity and smoking cessation can reduce CVD risk universally. This signifies a necessary shift toward being proactive in the prevention of CVD. …

Consumer Perceptions Of Farmers Market Performance: A Comparative Analysis Across States And Age Groups, Azita Varziri, Timothy A. Woods, Shuoli Zhao

Agricultural Economics Faculty Publications

The USDA Agricultural Marketing Service has invested in direct-to-consumer initiatives to expand local food consumption and enhance farmers market opportunities. To evaluate the effectiveness of these efforts, this study employs a sample-selected ordered probit model to analyze consumer perceptions of farmers market performance across four states in the Southeast United States. Specifically, we examine differences in awareness and performance of product quality, diversity, and market infrastructure. Awareness and perceptions vary significantly by age, other demographics, and state. These insights provide market organizers and policymakers with data-driven strategies to tailor marketing efforts and improve engagement across diverse consumer segments.

Investigation Of Factors Influencing Electric Vehicle Adoption In Indonesia: Ev Owners’ Perspectives, Desrina Yusi Irawatia, Nur Aini Masruroh, Nur Mayke Eka Normasari

ASEAN Journal on Science and Technology for Development

Electric vehicle (EV) uptake in Indonesia remains markedly below policy benchmarks. This study applies the Unified Theory of Acceptance and Use of Technology version 3 (UTAUT3), an extension of UTAUT2 that incorporates personal innovativeness as an additional construct to examine its impact on both behavioral intention and actual EV adoption within the Indonesian context. Unlike studies that typically survey the general public, this study focuses on actual EV users and owners, providing more representative and responsive insights into real-world EV usage. A total of 208 respondents participated, with 135 from the Jabodetabek area and 73 from Surabaya. The UTAUT3 framework …

Anticancer Efficacy Of Methyl-Cantharidimide (Mca) In Liver Cancer, Yidong Li

Theses and Dissertations

Cancer is still one of the major public health challenges worldwide to the present day. Multi-drug resistance (MDR) that occurs in cancer chemotherapies is a non-negligible cause of cancer treatment failure and tumor recurrence. Overexpression of ATP-binding cassette (ABC) transporters is one of the most frequent mechanisms of MDR occurrence. Therefore, the establishment of a chemotherapy agent whose anticancer efficacy would not be affected by the overexpression of ABC transporters is in demand. Methyl-cantharidimide (MCA), a cantharidin (CTD) analog, has been approved in China for clinical liver cancer treatment. This study revealed that MCA was efficacious in the ABCB1-, ABCG2-mediated …

Fractional Ablative Co2 Laser Versus Screw Threads In Post-Traumatic Atrophic Linear Scars, Eman Emad Elfeel, Marwa Zohdy Ali, Shereen Ezzalregal Alashry, Sherihan Hassan Allam

Mansoura Medical Journal

Background Scar is the result of the natural healing and reparative process, which are frequently accompanied by a few aesthetic, functional, and social impairments and may negatively affect quality of life. Fractional CO2 laser improves both the pigmentary and textural changes by removing thin layers of skin with minimal thermal damage. Thread lifting technology is a non-surgical facial rejuvenation technology that can minimize postoperative recovery period and scar from the incision Aim To compare the efficacy of fractional ablative CO2 laser therapy versus screw threads in post-traumatic atrophic linear scars. Methods This prospective randomized clinical study included 2 groups; group …

Adnexal Magnetic Resonance (Adnex –Mr) Scoring System; A New Standardized Method For Characterization Of Adnexal Masses Inside Mansoura University Hospitals, Nora Diaa Ali Okab, Basma Elged, Sherif Abdel Fatah Moustafa, Sieza Samir, Yasser.M. Mesbah

Mansoura Medical Journal

Background Gynecologic masses are the most common subject of gynecologic imaging. The usefulness of MRI is high in the characterization of adnexal masses. The ADNEX MR classification framework was launched to stratify sonographically identified adnexal masses in to five groups based on their probability of malignancy: grade 1 (no lesion), grade 2 (benign), grade 3 (probably benign), grade 4 (indeterminate), and grade 5 (probably malignant). Aim to determine the accuracy of the ADNEX MR scoring system in classification of adnexal masses at Mansoura University Hospitals (MUH). Methods A total of 48 female patients with 52 adnexal masses on ultrasound were …

Prevalence And Spectrum Of Genitourinary Anomalies In Children With Anorectal Malformations, Adnan El-Ghazaly, Tamer A. Wafa, Ahmed M. Negm, Adham W. El-Saied

Mansoura Medical Journal

Background: Anorectal malformations (ARMs) encompass a broad range of congenital defects and are frequently associated with genitourinary (GU) anomalies, which may contribute to long-term morbidity if undetected. This study aimed to determine the prevalence and spectrum of GU anomalies in children with ARMs, describe the urological and genital findings, and assess associations with sex and ARM type.

Patients and Methods: A prospective survey enrolled children diagnosed with ARMs at a tertiary pediatric surgery center. All patients underwent standardized clinical assessment, including focused genital examination and structured imaging. Abdominal ultrasonography (US) served as baseline screening for urinary tract anomalies. Voiding cystourethrogram …

Effects Of Fermented Soybean Products On The Human Gut Microbiome, Microbial Metabolism, And Host Physiology, Shannon Moncier

Biology Dissertations

Fermented soybean products (FSPs) contain soybean-derived substrates, fermentation-associated microorganisms, and bioactive compounds that may interact with the human gut microbiome and host physiology. However, despite the growing interest in FSPs, longitudinal human studies specifically evaluating traditional Korean-FSPs (K-FSPs), particularly among United States-based populations, remain limited. The objective of this work was to evaluate the effects of three K-FSPs, including Cheonggukjang, Gochujang, and Doenjang, on host physiological responses, gut microbial community structure, predicted microbial functional potential, and fecal butyrate responses in healthy adults. Three independent dietary intervention cohorts were conducted using FSP formulations produced either by traditional artisan methods (Product A) …

Investigation Of Genx Exposure To Pathways Associated With Colorectal Cancer Risk, Emily J. Ferguson

Theses and Dissertations--Toxicology and Cancer Biology

Per- and polyfluoroalkyl substances (PFAS) are persistent environmental contaminants widely detected in drinking water and food sources, resulting in chronic human exposure. Among these compounds, hexafluoropropylene oxide dimer acid (HFPO-DA), commonly known as GenX, has been introduced as a short-chain replacement for legacy PFAS such as perfluorooctanesulfonic acid (PFOS) and perfluorooctanoic acid (PFOA). Although GenX is believed to be a safer alternative, emerging research suggests it can still affect human health.

Colorectal cancer (CRC) is the third most commonly diagnosed cancer and the second leading cause of cancer-related death in the United States, highlighting the need to better understand environmental …

Uncovering The Mechanism Of Reep2-Mediated Emt-Driven Membrane Trafficking In Lung Adenocarcinoma, Kevin Fulp

Theses and Dissertations--Toxicology and Cancer Biology

Membrane trafficking is frequently disrupted during cancer progression, and the underlying mechanisms remain largely unknown. Currently, no effective drugs target dysregulated membrane trafficking for cancer treatment. Recent evidence has demonstrated that epithelial-to-mesenchymal transition (EMT) in lung adenocarcinoma (LUAD) employs a membrane trafficking program to coordinate cancer cell invasion and immunosuppression. To further dissect the pro-tumorigenic membrane trafficking program, we initiated an in vivo CRISPRi screen to assess more than 2,000 membrane trafficking-related genes in a syngeneic mouse LUAD model. We identified REEP2, an endoplasmic reticulum (ER) shaping protein, as a novel regulator of EMT-driven membrane trafficking. High REEP2 expression is …

Reep2-Driven Pro-Metastatic Secretion Promotes Lung Cancer Progression, Oluwafunminiyi E. Obaleye

Theses and Dissertations--Toxicology and Cancer Biology

Membrane trafficking plays a critical role in cellular function and is frequently dysregulated in cancer to promote metastasis. In lung adenocarcinoma (LUAD), the epithelial-to-mesenchymal transition (EMT) activating transcription factor, ZEB1, drives a pro-metastatic membrane trafficking program; however, the underlying molecular mechanisms remain poorly understood. Using a CRISPR interference (CRISPRi) in vivo screen of 2,099 membrane trafficking regulators in a syngeneic mouse model of EMT-driven LUAD, we identified REEP2 — an endoplasmic reticulum (ER) shaping protein — as a critical regulator of tumor progression. REEP2 mRNA expression correlates with poor prognosis, EMT signatures, and an immunosuppressive tumor microenvironment in LUAD patients. …

Assessing The Impact Of Local–State Revenue Shares And Property Tax Structure On Effective Farmland Tax Rates: Panel Evidence From Seven Midwestern States, 2009–2022, Imtiaz Amin Chy

Electronic Theses and Dissertations

Property taxes are the primary revenue instrument of local governments in the United States, yet the burdens they impose are distributed unevenly. Farmland, because it cannot be relocated, often absorbs a disproportionate share. This paper examines two related questions: what fiscal factors determine how heavily agricultural land is taxed, and do those tax rates capitalize into farmland asset values? Using an annual panel of Iowa, Illinois, Indiana, Kansas, Minnesota, Nebraska, and Wisconsin from 2009 to 2022, we first document how each state allocates property tax revenue across four major spending categories (education, public welfare, health, and utilities) finding that all …

Accelerating The Discovery Of The Genetic And Molecular Bases Of Neurodevelopmental Disorders In Vivo Using Breasi-Crispr, Claire Mae Kittock

Dissertations and Theses

Many neurodevelopmental disorders occur due to perturbations in prenatal brain development. More genetic variants associated with neurodevelopmental disorders are continuously being identified. However, there is a lack of understanding of the pathogenic mechanisms. Here, we describe a novel application of the technique Breasi-CRISPR in modeling specific patient variants in the embryonic mouse brain. Breasi-CRISPR is a highly efficient system combining in utero electroporation with CRISPR-CAS9 genome editing. We hypothesized that Breasi-CRISPR would be sufficient at inserting patient analogous variants into endogenous loci to be used as a new paradigm for modeling neurodevelopmental disorders. We aimed to test if Breasi-CRISPR could …

Practical Considerations For Conducting Canine Behavioral Science, Lucrezia Lonardo, Madeline H. Pelgrim, Jeffrey R. Stevens

Jeffrey Stevens Publications

Research on behavior in domestic dogs (Canis familiaris) has increased rapidly recently due to the interest in them as pets, working dogs, and model species for understanding evolution, domestication, and even human health. This interest in canine behavioral science has resulted in the creation of dozens of dog lab worldwide, with more starting every year. Our aim here is to review practical issues that potential dog researchers might consider when deciding how to set up a dog lab and conduct research in canine behavioral science. We hope that this can offer a path forward in promoting high-quality, cutting-edge …

A Comprehensive Statistical And Regional Analysis Of Lng-Powered Marine Vessels On Ghg Mitigation Strategies Considering Existing Bunkering Stations And Gwp Values, Canberk Hazar, Onur Yuksel, Murat Bayraktar

Journal of Marine Science and Technology–Taiwan

This study aims to quantify and analyse emissions from marine vessels that can operate on liquefied natural gas (LNG) but continue to use conventional fuels, largely due to the limited availability of LNG bunkering stations (BSs) over long distances. Four regions have been identified as having high concentrations of LNG-fueled vessels but limited access to operational BSs: the West Coast of the United States of America (USA), South Africa–Good Hope–Madagascar, Northwest Africa, and Brazil. This selection is based on the geographical distribution of these ships and the existing infrastructure. Hourly greenhouse gas (GHG) emissions have been calculated by considering the …

Temporal Avoidance By Gray Foxes Reflects Growing Pressure From Expanding Coyotes In The Neotropics, Madison Odenbach

Undergraduate Theses, Professional Papers, and Capstone Artifacts

Coyotes (Canis latrans) are rapidly expanding their range towards South America, potentially threatening the world’s highest canid diversity residing on that continent. A better understanding of their interactions with native species is needed to anticipate potential competitive impacts. In Costa Rica, native gray foxes (Urocyon cinereoargenteus) likely occupy a similar niche to the coyote newcomer, yet little data exists on their interactions in tropical ecosystems. We hypothesized the two species separate temporally to minimize the potential for competition in our study area, given preliminary data suggesting high spatial overlap (57.1% of coyote-detected sites overlap with fox-detected …

Genomic Determinants Of Appetite Regulation And Their Role In Obesity Risk, Timothy J. Renier

Dartmouth College Ph.D Dissertations

Over 40% of US adults and 20% of children live with obesity. It is related to increased risks of diseases including type II diabetes and cancer, is a source of harmful stigma, and impairs child development. Appetitive traits describing patterns of food approach and avoidance behaviors may serve as windows into the underlying pathophysiology of obesity. As both obesity and appetitive traits are heritable, common genetic drivers of obesity may lead to phenotypic obesity through appetitive traits as a disease mechanism. Additionally, GLP-1 receptor agonists (GLP-1RAs) have been shown to exert their profound clinical effects in part by modulating appetite. …

Advancing Food Equity Through Explainable Ai (Xai): Identifying Place-Based Factors And Conditions Of Food Security, Leslie Hoglund, Hyoshin Park

Health Behavior, Policy & Management Faculty Publications

Food behaviors, food security, and their association with socioeconomic factors constitute a critical area of study with implications for public health, economic stability, and social equity. Understanding these relationships are essential for developing effective policies and interventions that promote sustainable, healthy food systems and greater food equity. This paper employs explainable artificial intelligence (XAI) methods to identify key features influencing household food behaviors. The insights gained from the XAI analysis are further utilized in conjunction with inverse reinforcement learning (IRL) to examine expert behaviors related to eating habits satisfaction. The XAI results reveal that household health conditions, spending patterns, and …

Nutrient-Driven Transcription Factor-Dna Interactions And Their Signaling Mechanisms In Metabolic Disease, Evelyn A. Bates

Theses and Dissertations--Nutritional Sciences

Metabolic homeostasis is maintained through tightly coordinated signaling networks that integrate diverse physiological inputs. Central to this regulation are transcription factors, which sense nutrient, hormonal, and vitamin-derived signals and translate them into coordinated gene expression programs. Transcription factor activity is governed by DNA binding, recruitment of coregulatory proteins, and interactions with specific response elements in gene promoters, which allows them to selectively modulate diverse metabolic pathways. Owing to their multifactorial and tunable nature, transcription factors have emerged as promising targets for the treatment and diagnosis of metabolic diseases, including metabolic dysfunction-associated steatotic liver disease (MASLD). This dissertation explores the molecular …

The Effect Of Second-Order Slip In Hydromagnetic Eyring-Powell Flow: A Bvp4c Study, Ch. Maheswari, B. Naga Lakshmi, G. Dharmaiah, R. Mohana Ramana, B. Reddappa

Mansoura Engineering Journal

The steady-state Eyring–Powell boundary layer fluid flow past a moving surface is examined, considering various parameter implications. These include the wall mass transfer parameter (0.5  C   2.0), the magnetic field parameter (5  Mn 20), the velocity ratio parameter (0.1  λ1  0.7), Eyring-Powell fluid parameters (0.5 K 2 & 0.1 E 0.4), as well as the first-order velocity slip (0.1 γ 1.1) and second-order velocity slip (0.1 δ  0.4). By employing appropriate similarity transformations, the PDEs are reduced to a set of non-linear ODEs, which are subsequently solved using the bvp4c numerical technique and, an implication of key factors on shear …

Preserving The Identity Of Historic Coptic Orthodox Monasteries In Egypt Under Contemporary Pressures: Case Studies Of St. Bishoy And St. Anthony Monasteries, George Medhat Adeeb, Mohamed Khairy Amin, Mohamed Ahmed Al-Sherbiny

Mansoura Engineering Journal

Historic Coptic Orthodox monasteries in Egypt (HCOMEs) represent an important component of Christian heritage, integrating architectural value with enduring spiritual traditions that reflect both tangible and intangible dimensions. However, contemporary development and changing functional demands are generating transformations that may affect their identity if not carefully managed. Despite extensive scholarship, limited research has systematically examined these transformations through a structured evaluative approach. This study addresses this gap by developing and applying an analytical and evaluation framework to examine how contemporary pressures influence the historical, architectural-spatial, and spiritual identity of HCOMEs, and to assess the effectiveness of monastic responses. The framework …

Secondhand Books Industry Between Extinction And Challenges Of The New Millennium: Insights Urban And Architectural Considerations From Al-Azbakeya Book Market., Ahmed M. Selim, Heba M. Gaber, Nehal M. Elmahdy

Mansoura Engineering Journal

At the beginning of the 21st century, the printed and secondhand book industry has faced enormous challenges, particularly after the COVID-19 pandemic, when book consumers sought to engage directly with e-books. The Azbakeya Book Market in Egypt is one of the markets that confronts this challenge, while maintaining its distinctive historical character. This study aims to rehabilitate the secondhand book market in Al-Azbakeya district as a model that simulates the potential urban and sustainable architectural design strategies for reviving this industry. The study framework was comprised into four sections. First, "desk-based research" was conducted to discuss the secondhand book industry …

Do Magnesium Ions Have Similar Effects As Calcium Ions On Resting Membrane Potential?, Anthony Hana, Youngwoo Kim, Joy Bidros, Katie Neglia, Robin L. Cooper

Biology Faculty Publications

Maintaining a membrane electrical potential of biological cells is a dynamic process, as some cells have a continually changing potential, like pacemaker cells, while other cells may function with large or small changes in the membrane potential. Additionally, some cells may change their electrical potential when stimulated or inhibited by electrical signals, chemical compounds, or both—either simultaneously or episodically. The persistent leak of K⁺ through two-pore-domain potassium channels (K2P) and of Na⁺ through Na⁺ leak channels (NALCNs) and the action of pumps and exchangers are primarily responsible for maintaining a resting potential. Ca²⁺ ions are known …

Induced Degradation Of G-Protein-Coupled Receptors Through Proteolysis Targeting Chimeras, Victoria Saca

Student Theses and Dissertations

G protein-coupled receptors (GPCRs) are a family of heptahelical transmembrane proteins that can modulate various cellular signaling pathways and therefore are attractive drug targets. Despite high mutational rates of GPCRs in cancer there are few oncology FDA-approved anti-GPCR drugs, leaving a gap in the therapeutic landscape. Drug discovery platforms such as targeted protein degradation (TPD), provide opportunities to target oncogenic proteins by direct modulation of protein expression. TPD methodologies can be used to target oncogenic GPCRs that are overexpressed or constitutively active, as in the case of constitutively active mutants (CAMs), which have roles in cancer progression. TPD methods include …