Digital Commons Network^™

Invertebrate Communities Associated With Three Early Phases Of A Prairie Restoration Project, Tracy R. Evans, Meredith J. Mahoney, E. D. Cashatt, Bryon W. Cross, Geert R. De Snoo, C. J.M. Musters

The Great Lakes Entomologist

While specific invertebrate groups have been studied in prairie restorations, there are few studies that look at terrestrial invertebrate assemblages. We surveyed invertebrates in three phases of plant restoration that were part of a larger restoration project. This cross-sectional study of invertebrate recovery at two, four and five years post-restoration shows there was no overall difference in invertebrate taxa richness and diversity between restoration phases. Overall abundance was greatest in the most recently restored area. Richness, diversity and abundance of six functional groups did not differ. The conclusion is that all phases are still characterized by pioneer invertebrate assemblages, and …

A Synopsis Of The Cimicoidea (Heteroptera) Of Michigan, Daniel R. Swanson

The Great Lakes Entomologist

An overview of the 20 species of Cimicoidea (Anthocoridae, Cimicidae, Lasiochilidae, Lyctocoridae) found in Michigan is presented, along with identification keys, distribution maps, and relevant literature. Five new state records for the following species, representing three of the four cimicoid families found in Michigan, are presented: Oeciacus vicarius Horváth (Cimicidae: Cimicinae), Cimexopsis nyctalis List (Cimicidae: Haematosiphoninae), Amphiareus obscuriceps (Poppius) (Anthocoridae), Cardiastethus borealis Kelton (Anthocoridae), and Lyctocoris stalii (Reuter) (Lyctocoridae).

Redescription Of The Female Of Oobius Depressus (Girault) (Hymenoptera: Encyrtidae), Newly Found In Michigan, U.S.A., Serguei V. Triapitsyn, Toby R. Petrice, Vladimir V. Berezovskiy

The Great Lakes Entomologist

A female of Oobius depressus (Girault) (Hymenoptera: Encyrtidae), collected in a modified Malaise trap in the canopy of a black locust tree, Robinia pseudoacacia, at Rose Lake State Wildlife Area in Bath Charter Township, Clinton Co., Michigan, U.S.A., is re-described and illustrated. This species was previously known only from the type series of headless specimens of both sexes from the type locality in Morristown, Henry Co., Illinois, U.S.A. These type specimens were from a single cohort of locust borer, Megacyllene robiniae (Forster) (Coleoptera: Cerambycidae), eggs collected and reared over one hundred years ago. An updated taxonomic key to females of …

Generation Of A Transgenic Vector For The Purpose Of Transforming Charcoal Rot Resistance Into Soybeans (Glycine Max), John M. Bear Jr

Electronic Theses & Dissertations

Charcoal rot, caused by Macrophomina phaseolina, is among the most devastating fungal pathogens affecting G. max, particularly in drought-susceptible regions such as the midwestern United States. Traditional agricultural practices have included crop rotation and irrigation, which have proved ineffective or economically unfeasible. Charcoal rot infects over 500 other species of plants and can overwinter as dormant sclerotia which can germinate once soybeans are re-planted and infect young plants. As a result, transgenic solutions have offered a promising alternative for the development of disease-resistant soybeans. This thesis documents the construction of a transgenic vector to express BOZO, a …

Stress-Related Phenotypic Differentiation Identified By Motivational Behaviors In Male Mice, Renee A. Brummels

Honors Thesis

Social stress substantially contributes to psychological disorders, including anxiety, depression, and post-traumatic stress disorder. Recognizing these disorders affect millions, it is essential to understand social stress’s impacts on behavior and how these behaviors vary among affected populations. We address these questions through a social defeat/avoidance paradigm, the Stress-Alternatives Model (SAM), which demonstrates defeat (Stay) and avoidance (Escape) responses across species, including fish, rats, hamsters, and mice. Avoidance of social conflict in the SAM is possible by utilizing escape routes for smaller test mice during aggressive interactions. Additionally, measurements of SAM motivational behaviors, like time near and nose pokes into escape …

Evaluating Simulated Threecornered Alfalfa Hopper (Hemiptera: Membracidae) Damage In Cotton (Malvales: Malvaceae) And Identifying Conducive Environments, Reece Starnes Butler

Theses and Dissertations

This research evaluated the effects of simulated threecornered alfalfa hopper (TCAH), Spissistilus festinus (Say), damage in cotton and aimed to identify environments that may contribute to an increase in TCAH injury. During the 2023 and 2024 growing season, simulated TCAH damage was imposed at 0%, 10%, 20%, 30%, 40%, and 50% at the three and six leaf growth stage. Agronomic data included plant heights, node counts, and plant mapping, which was collected throughout the growing season, while lint yield was recorded at harvest. Damaged plants exhibited reduced plant heights and node counts, with an increase in nodes above cracked boll …

Exploring Sustainable Biopolyesters: Synthesis From 1,4-Butanediol And Aliphatic Diacids, Vinashkumar Jayantibhai Chaudhari

Electronic Theses & Dissertations

In this thesis, we applied 1,4-butanediol, which is an emerging biobased and low-cost monomer, was synthesized with a series of aliphatic dicarboxylic acids, namely succinic acid, adipic acid, and sebacic acid using melt polycondensation to Biobased polyesters. The resulting polymers poly(butylene succinate) (PPeS), poly(butylene adipate) (PPeA), and poly(butylene sebacate) (PPeSe) chemical structures were analysis through Fourier transfer infrared spectroscopy(FT-IR), ¹H nuclear magnetic resonance (NMR) revealed the elimination of side reactions in the obtained polyesters. Differential scanning calorimetry (DSC) was employed to analyze physical properties, and the impact of dicarboxylate chain length and long short diol on crystalline structure and …

Trade-Off Between Limonene & Geraniol-Based Reprocessable And Non-Reprocessable Epoxy Thermosets: Role Of Aliphatic Diamines In Polymer Networks Design, Priyankkumar Kanubhai Patel

Electronic Theses & Dissertations

The growing demand for sustainable materials, driven by environmental concerns and the rapid depletion of fossil fuels, has garnered significant attention in the fabrication of bio-based epoxy thermosets as renewable alternatives to petroleum-derived thermosetting polymers. The present study focuses on the synthesis and characterization of epoxy prepolymer resins derived from limonene and geraniol via a two-step process, followed by the fabrication of thermally cross-linked flexible epoxy thermoset films using various traditional aliphatic diamine curing agents, namely 1,2-ethylenediamine (EDA), 1,4-butylenediamine (BDA), and 1,6-hexamethylenediamine (HDA). Additionally, the incorporation of cystamine, a disulfide-containing diamine, enabled the formation of a covalent adaptable network through …

Zn–Assisted Synthesis Of M (Mn/Fe/Co/Ni)-N-C Catalysts For Multifunctional Electrochemical Activity, Kemila A. Chaudhary

Electronic Theses & Dissertations

The design and development of atomically dispersed M-N-C catalysts (metal (M) supported on an nitrogen-carbon (NC) matrix with high multifunctional electrocatalytic performance is desirable but proved to be very challenging. Herein, we synthesized M-N-C catalysts (M = Fe, Co, Mn, and Ni) using Zn-assisted high temperature treatment and characterized using various techniques. The prepared catalysts were tested for their electrocatalytic performance towards oxygen and hydrogen evolution reaction (OER and HER) as well as oxygen reduction reaction (ORR) in alkaline media. The results indicated that Mn-N-C catalyst showed higher performance towards both the ORR (E_1/2 = 0.90 V) and …

Analyzing Ballast Water Treatments, Invasive Species, And Pathogens, And A Decade-Long Analysis On The San Francisco And Baltimore Ports, Jasmin Ibarra-Cortes

Master's Projects and Capstones

Installation of advanced technologies to treat ballast water on ships is necessary to meet current ballast water management standards and reduce the secondary spread of invasive species during intracoastal voyages. This research includes a literature review on non-native aquatic species, particularly diapausing eggs, and available treatment methods for installation. A comparative analysis from 2014 - 2024 on bulker, tanker, and container vessels arriving coastwise to the ports of San Francisco and Baltimore was performed to evaluate treatment installation trends, traffic patterns, and the effectiveness of treatment(s) on targeting diapausing eggs. Use of ultraviolet (UV) radiation combined with filtration has increased …

High Kynu Expression Is Associated With Poor Prognosis, Keap1/Stk11 Mutations, And Immunosuppressive Metabolism In Patient-Derived But Not Murine Lung Adenocarcinomas, Ling Cai, Thomas J Rogers, Reza Mousavi Jafarabad, Hieu Vu, Chendong Yang, Nicole Novaresi, Ana Galán-Cobo, Luc Girard, Edwin J Ostrin, Johannes F Fahrmann, Jiyeon Kim, John V Heymach, Kathryn A O'Donnell, Guanghua Xiao, Yang Xie, Ralph J Deberardinis, John D Minna

Faculty, Staff and Student Publications

Background/Objectives: We aimed to discover genes with bimodal expression linked to patient outcomes, to reveal underlying oncogenotypes and identify new therapeutic insights in lung adenocarcinoma (LUAD).

Methods: We performed meta-analysis to screen LUAD datasets for prognostic genes with bimodal expression patterns. Kynureninase (KYNU), a key enzyme in tryptophan catabolism, emerged as a top candidate. We then examined its relationship with LUAD mutations, metabolic alterations, immune microenvironment states, and expression patterns in human and mouse models using bulk and single-cell transcriptomics, metabolomics, and preclinical model datasets. Pan-cancer prognostic associations were also assessed.

Results: Model-based clustering of KYNU expression outperformed median-based dichotomization …

Plastic In The Pack: Assessing Microplastic Risk To Urban Coyotes (Canis Latrans) In The San Francisco Bay Area, Elaina E. Herrmann

Master's Projects and Capstones

Microplastics are an emerging concern worldwide. However, their impact on terrestrial mammals, such as Canis latrans (coyotes), has yet to be extensively studied. Coyotes are unique in their ability to adapt to urban environments and have recently begun bridging the gap between marine and terrestrial food webs. This research examines the potential sources and impact of microplastic exposure in urban coyotes in the San Francisco Bay Area and possible food web impacts through literature synthesis and Geographic Information System (GIS) mapping. This research examined potential mechanisms for direct and trophic microplastic uptake by coyotes resulting from the anthropogenic, terrestrial, and …

Balancing Burrowing Owl (Athene Cunicularia Hypugaea) Protections While Maintaining Renewable Energy Development Within The San Joaquin Valley, Madison Lenzi Mcginn

Master's Projects and Capstones

Balancing the needs of wildlife and people has long been a complex challenge. The San Joaquin Valley exemplifies this tension, as space for agriculture and urbanization compete for land use of native grasslands– often resulting in the conversion of grasslands for agriculture fields, commercialized areas, or energy development. With an increasing number of agricultural landscapes going fallow and the passing of SB-100 in 2018, solar farm development has emerged as an attractive option to offset financial losses from unproductive farmland. However, the same landscapes favored for solar development also provide suitable habitat for sensitive species such the western burrowing owl …

Effects Of Different Forms Of Dietary Zinc On Weaned Steers When Fed Prior To Weaning, Christopher Lane Addison

Theses and Dissertations

Zinc (Zn) is crucial for many life sustaining processes within the body. Additional Zn is known to be beneficial during certain periods of increased stress. Weaning is one of the most stressful periods of beef calves’ lives. Due to this it was hypothesized that additional zinc in an organic form would increase weight gain and immune response compared to additional zinc in an inorganic form or no added zinc. No differences were found for ADG, Th score, IgG concentration, or mineral concentration among treatments. However, there was a novel and unexpected finding when there was no difference found in preweaning …

Evaluation Of Plant Growth Regulators On Sweetpotato Slip Propagation, Kerington Lyn Bass

Theses and Dissertations

Sweetpotato production is often impacted by high mortality of slips during transplantation to the field, particularly with greenhouse-grown slips. These slips develop thinner stems, thus being more vulnerable to transplant shock, resulting in reduced establishment. To address this issue, the study evaluated the effects of four plant growth regulators on the morphological and anatomical characteristics of sweetpotato slips. In the first experiment, external growth parameters were recorded over four weeks, followed by post-harvest assessments of plant and root material. In the second experiment, the widths of various stem structures in stained cross-sections of slip stems were measured. Results showed that …

The Effects Of Ataxin-1 Overexpression In The Central Nervous System On Specific Behavioral And Physiological Characteristics Of Drosophila Melanogaster, Sydney Davis

Theses and Dissertations

Spinocerebellar ataxia type 1 (SCA1) is a dominantly inherited neurodegenerative disease caused by a CAG repeat expansion in the ataxin-1 gene, which results in an abnormally long polyglutamine tract. Similarly, overexpression of wildtype Ataxin-1 (wt-Atx-1) in Drosophila melanogaster leads to neurodegeneration, reduced lifespan, and impaired negative geotaxis. Observations include disruptions in sleep-wake cycles, increased oxidative stress biomarkers (like protein carbonyls), and dysregulation of developmental genes such as armadillo (arm) and nemo kinase (nmo). Additionally, elevated expression of SUMOylation-related genes (Aos-1, Uba2, and dSmt3 or dSumo) and increased SUMO protein levels indicate enhanced SUMOylation in the brain. Quantitative analysis of neuronal …

Assessment Of Pagp Gene Function In Avian Pathogenic Escherichia Coli Pathogenicity Using Different Challenge Models, Hailey Fugate

Theses and Dissertations

Avian pathogenic Escherichia coli (APEC) is a leading cause of morbidity and mortality in poultry, contributing an estimated $40 million in annual losses in the U.S. broiler industry alone, and contributing to growing concerns over antibiotic resistance. This study investigated the role of the pagP gene, which encodes a lipid A palmitoyl transferase involved in immune evasion, using both a broiler chick and embryo challenge model. Deletion of pagP significantly reduced mortality and lesion severity in broilers, indicating its role in APEC pathogenicity. However, in embryos, pagP deletion had minimal effects on survival and bacterial colonization, suggesting alternative virulence mechanisms …

Impact Of An Automated Brush System On Intensively Managed Lactating Dairy Cattle Behavior, Production, And Stress Physiology, Allison Lyn Griffin

Theses and Dissertations

The dairy industry has increasingly prioritized improving animal welfare for intensively housed cows. This study evaluated the effects of environmental enrichment (EE) using an automated brush system on stress physiology, behavior, and productivity of lactating dairy cows. Fifty-nine pregnant and lactating dairy cows were divided into two groups: CON (no brush) and TRT (automated brush system). Saliva samples were collected to measure cortisol concentrations, and behaviors were monitored in the morning (07:00 h - 11:00 h) and evening (19:00 h - 23:00 h) using high-resolution cameras. The results indicated that cows with brush access had lower cortisol concentrations, suggesting reduced …

Exploring Sexual Dimorphism In A Drosophila Melanogaster Model Of Spinocerebellar Ataxia Type 1, Madelyn Hunter

Theses and Dissertations

Neurodegenerative diseases affect over 36 million people and are characterized by loss of motor control, neuronal degradation, and behavioral changes. The most common of these diseases exhibit sexually dimorphic effects. In this study, Spinocerebellar ataxia type 1 (SCA1) was modeled in the Drosophila central nervous system using the yeast hybrid UAS/GAL4 system. Locomotor activity and clock gene expression was monitored for male and female SCA1 and control flies. Clock genes such as per, tim, Clk, and cyc were monitored over a 24-hour period using qRT-PCR, and distinct sexual dimorphism was detected in locomotor activity and in gene expression for SCA1 …

Taking Flight: Utilizing Unmanned Aerial Systems To Sample Insects In Soybean, William "Covey" Lockhart

Theses and Dissertations

Insect scouting in soybean is a labor-intensive task but is needed throughout the season to minimize losses. When soybeans are tall, soils are wet, or soybeans are planted in narrow rows, walking through these fields can be difficult. To overcome these limitations of manual sampling, an unmanned aerial vehicle (UAV) sampling platform was developed that can collect insects in a sweep net attached to the bottom of a UAV. This system has been named the “Drone Insect Net Sampling System” (DINSS). Previous research with this system has shown positive correlations to traditional manual sampling methods. To improve this system, modifications …

The Effect Of X-Ray Irradiation Technology On Salmonella Spp. And Quality Parameters Of Ready-To-Bake Chocolate Chip Cookie Dough, Kala Grace Morris

Theses and Dissertations

Salmonella contamination in cookie dough poses a threat to consumers. This study investigated the effect of X-ray irradiation on Salmonella-inoculated ready-to-bake chocolate chip cookie dough and its impact on dough quality. A Salmonella cocktail (~10 log CFU/g) was prepared and inoculated into cookie dough samples (~6.5 log CFU/g) and then treated with X?ray irradiation (0.25, 0.5, and 1 kGy). Salmonella decreased by 1.15, 1.79, and 2.49 log CFU/g, at an exposure of 0.25, 0.5, and 1 kGy, respectively. Quality parameters (pH, aw, and colorimetry) were evaluated for two brands. No statistical difference (P>0.05) in pH was observed for Brand …

Evaluation Of Broiler Performance And Processing Metrics Through Sodium Bisulfate Application To Litter And Varying Inclusions In Feed, Samantha Suzanne Plocher

Theses and Dissertations

Poultry producers must continuously evaluate current practices and determine new ones to further optimize broiler production. Sodium bisulfate (SB) is used as a poultry litter amendment (SBL), but also in human food to control pH; thus, initiating interest for SB in feed (SBF) for performance and gut health benefits. This thesis utilized common diets varying in SBF (Chapters II and III) and SBL (Chapter II; SBL1- d 0 and SBL2 – d 0 and 18) to optimize 40 d broiler performance, litter quality, and d 41 processing metrics. For Chapter II, d 0 SBL1 dramatically lowered litter pH, while minimally …

Ecology And Management Of Alligatorweed (Alternanthera Philoxeroides) And An Associated Thrips Biological Control Agent (Amynothrips Andersoni), Samuel Alec Schmid

Theses and Dissertations

Alligatorweed (Alternanthera philoxeroides), native to South America, is a highly invasive aquatic macrophyte that is globally distributed and particularly problematic in the southeastern United States. In the United States, alligatorweed management has a long history, particularly of chemical and biological control. Alligatorweed management saw early success with a South American flea beetle (Agasicles hygrophila), but this agent’s utility has waned. Despite the long history of alligatorweed invasion, several important research questions remain, and the research in this dissertation seeks to answer some of these questions. This research focuses on multiple and diverse aspects of alligatorweed biology, ecology, and management: (1) …

Assessing The Kinematics And Kinetics About The Knee With An Isokinetic Dynamometer And Force Plates During Field-Based And Lab-Based Trials., Samantha Ann Searles

Theses and Dissertations

The incidence rate in knee injuries has increased over the last 20 years, with women at a 2-8 times higher incidence rate compared to men. This is likely a result of lower neuromuscular control of the trunk, generating less hip muscle activation, and more reliance on the quadriceps, alongside reduced hip, knee, and dorsiflexion angles, paired with increased knee valgus angles, ground reaction forces (GRF), hip adduction, and knee rotation upon landing from jumps. The focus of this study is to determine if knee kinetics and kinematics during field- and lab-based trials are associated with knee injury predictors. Theoretically, if …

Effects Of Environmental Stress And Antimicrobials On The Health, Microbiome, And Product Shelf-Life Of Channel And Hybrid Catfish, Guyue Tang

Theses and Dissertations

Two studies investigated various aspects of channel and hybrid catfish production and processing. The first study examined the effects of ammonia stress (15 mg/L vs. 1 mg/L control) on growth performance and fillet quality. While channel catfish demonstrated superior growth, ammonia stress minimally impacted the sensory profiles of both species. Gut microbiota analysis revealed species-specific differences, with Lactococcus lactis dominating hybrid catfish communities. The second study evaluated buffered vinegar treatment (1.5%) on fillet shelf life during refrigerated storage. Vinegar-treated fillets maintained better microbiological quality, with aerobic plate counts remaining below 7 log cfu/g through day 6, while untreated fillets exceeded …

Combined Effect Of Chitosan And Lemon Essential Oil-Infused Slush Ice On The Shelf Life Of Channel Catfish, Dianna Wilson

Theses and Dissertations

The objective of this research was to investigate the biopreservative effects of chitosan and lemon essential oil in slush ice treatments on the microbial shelf life of refrigerated catfish fillets. Catfish fillets were stored in slush ice treatments containing chitosan (CH), lemon essential oil (LO), a combination (CH+LO), water (CTRL), and no slush (NS) for three d at 4 C, then stored at 4C for twelve d post-slush. The growth of background microflora in catfish fillets treated with CH and CH+LO was reduced (P<0.05), maintaining total aerobic counts below 4.5 log CFU/g, while the control reached spoilage levels (>7 log CFU/g) by Day 6. Chitosan effectively extended the microbial shelf life but lowered pH, …

Myxozoan Diversity And Life Cycles In Catfish Production Ponds In Mississippi, Usa, Ethan Woodyard

Theses and Dissertations

Catfish aquaculture is a critical industry in the United States and Mississippi in particular. The industry is beset by depredation, rising feed costs, competition with imports, and loss to disease. Chief among these is the myxozoan parasite and causative agent of proliferative gill disease, Henneguya ictaluri. In chapter 2, novel molecular markers for greater resolution in species identification of these parasites are provided, allowing more robust life cycle inference, species-level identifications, and inference of evolutionary relationships through molecular phylogenetic analyses. Chapters 3–5 apply the approaches developed in Chapter 2 to a broad array of taxa beyond catfish-infecting Henneguya spp. These …

Targeting The Beiging Of Muscle-Associated Adipose Tissue To Promote Muscle Regeneration, Jacob C. Parson

WUSM Theses and Dissertations – All Programs

The expansion of intra-muscular adipose tissue (IMAT) between myofibers and epi-muscular adipose tissue (EMAT) outside of the muscle boundary accompanies numerous pathologies including diabetes, muscular dystrophy, and aging. IMAT and EMAT are anatomically juxtaposed to myofibers. Some evidence supports that IMAT and EMAT impede muscle function. This is potentially due to paracrine factors that are secreted by IMAT/EMAT. Different adipose depots can be classified in numerous ways including where they are located anatomically as well as the factors in which they secrete. Unlike classical white fat, adipose depots classified as brown or beige, noted by the marker uncoupling protein-1 (UCP-1) …

Heterologous Expression And Application Of A Thermophilic Lytic Polysaccharide Monooxygenase, Le Xie, Longyun Peng, Yun Hu, Hongyan Ren, Haiyan Sun, Fubao Sun

Journal of Food Science and Biotechnology

[Objective ] This study aims to investigate the application of a novel thermophilic lytic polysaccharide monooxygenase (LPMO ) in the efficient cellulase-mediated hydrolysis of lignocellulosic substrates.[Method ] The thermophilic enzyme mgLPMO 10,derived from a compost metagenome,was heterologously expressed in Escherichia coli (E.coli ).Its expression level was improved by modifying the signal peptide of the expression vector and induction conditions (temperature,IPTG concentration,and induction time ).The enzyme was then characterized in terms of the optimal temperature,optimal pH,thermal stability,pH stability,and the effects of metal ions on its enzyme activity.Subsequently,the synergistic hydrolysis efficiency of mgLPMO 10 with two thermophilic endoglucanases (TnCelB and DtCelB ) …

Preparation , Performance , And Application Of A High-Performance Affinity Chromatography Medium Based On Staphylococcus Aureus Protein A, Zihao Guo, Chaoqing Yan, Haifeng Xia

Journal of Food Science and Biotechnology

[Objective ] Staphylococcus aureus protein A (SPA ) is widely used for the purification of monoclonal antibodies due to its high specificity for immunoglobulin G (IgG).However,the high costs of commercial media have limited the further development of SPA.To address this issue,this paper aims to prepare a high-performance affinity chromatography medium based on SPA as the substitute of introduced affinity chromatography media.[Method ] SPA expressed by the recombinant strain was purified via Ni2+ affinity chromatography and then coupled to the Rigose HF matrix to prepare an self-prepared affinity chromatography medium.[Result ] The self-prepared medium had a static saturated adsorption capacity of …