Digital Commons Network^™

The Perfect Pairing: Early Stand Dynamics In Conventional Aspen And Mixedwood Forests In Northern Minnesota, Usa, Chelsea C. Semper, John M. Zobel, Sawyer S. Scherer, Michael R. Reinikainen, Matthew B. Russell, Tyler S. Gifford, Marcella A. Windmuller‑Campione

Aspen Bibliography

In North America, mixedwood management is being increasingly considered by natural resource managers as an alternative to single species management for a variety of ecological, economic, and cultural reasons. In northern Minnesota, USA, natural resource managers are increasingly using treatments to establish mixedwood forests in aspen (Populus tremuloides Michx, Populus grandientata Michx) dominated ecosystems by planting white spruce (Picea glauca (Moench). However, little quantitative information is known regarding differences between stand structure and composition in conventional aspen and aspen-white spruce during early stand development. To provide greater clarity for managers, we sampled 40 aspen stands across a chronosequence …

Protocol For Establishing Genetically Engineered Murine Lung Organoids Mimicking Cell Plasticity And Regeneration, Bongjun Kim, Jae-Il Park

Faculty, Staff and Student Publications

Lung endothelial cells (LuECs) play crucial roles in lung regeneration and support lung organoids (LOs). However, detailed guidelines for LO culture using LuECs have not been reported yet. Here, we present an efficient protocol for generating and culturing murine LOs that mimic regeneration. We describe steps for the isolation of LuECs and lung epithelial cells, followed by LO culture. We also describe how to establish genetically engineered LOs using viral transduction. For complete details on the use and execution of this protocol, please refer to Kim et al.

Usp37 Counteracts Hltf To Protect Damaged Replication Forks And Promote Survival Of Brca1-Deficient Cells And Parp Inhibitor Resistance, Mengfan Tang, Siting Li, Ziqiang Zhu, Chao Wang, Shuai Ding, Huimin Zhang, Min Huang, Sarah Keast, Zhen Chen, Ling Yin, Litong Nie, Dan Su, Xu Feng, Junjie Chen

Faculty, Staff and Student Publications

Poly(ADP-ribose) polymerase inhibitors (PARPi) have greatly improved survival of cancer patients harboring BRCA1 mutations. However, therapy resistance develops via either restoration of homologous recombination or replication fork stabilization. Therapeutic targets to overcome PARPi resistance are critically needed. We identified the deubiquitinase USP37 as a key determinant of PARPi toxicity in BRCA1-deficient cells via whole-genome CRISPR screens. USP37 ablation enhanced PARPi sensitivity in BRCA1-deficient cells and also overcame PARPi resistance due to 53BP1 loss. USP37 interacts with and deubiquitinates replication protein A (RPA) at stalled replication forks to limit excessive RPA accumulation, progressive RPA exhaustion, and the conversion of RPA-coated single-stranded …

Protocol To Analyze Trna And Rrna Processing Using Biotin-Labeled Probes, Sseu-Pei Hwang, Katherine Barondeau, Catherine Denicourt

Faculty, Staff and Student Publications

Mature tRNAs and rRNAs are derived from larger precursor transcripts through intricate endo- and exonuclease processing steps. Here, we present a protocol to analyze rRNA and tRNA processing using total RNA extracted from human cells. We describe steps for separating RNA by denaturing electrophoresis, followed by northern blotting. We detail procedures for detecting pre-rRNA intermediates or specific tRNA species using biotin-labeled probes imaged via fluorescence or chemiluminescence. This non-radioactive, high-sensitivity assay enables the investigation of rRNA and tRNA expression dynamics. For complete details on the use and execution of this protocol, please refer to Hwang et al.

Crayfish Microhabitats Provide A Close Association With Benthic Microbiomes To Support Cleaning Symbionts., Kyle J. Harris, John Hoverson, Matthew Becker, Matthew M. Cooke Mr

NEXUS: The Liberty Journal of Interdisciplinary Studies

Stream ecosystems promote variable microhabitats along a longitudinal gradient. These microhabitats support dynamic biotic assemblages. One such microhabitat in freshwater streams involves the external surface of crayfish. We investigated the microbiome of crayfish exoskeletons and the surrounding environment (water and substrate) in association with the presence and abundance of crayfish cleaning ectosymbionts (branchiobdellidans). These ectosymbionts actively consume the biofilm on crayfish as they live and reproduce in association with crayfish microhabitats. Sequenced microbes of the environment and crayfish were compared for dominant taxa. While the sample types (water, substrate, and crayfish) had significantly different microbiomes, there was not a significant …

Gardenia Jasminoides Extract As A Potential Neuroprotective Agent In An In Vitro Tauopathy Model, Inas Birekdar

Theses and Dissertations

Alzheimer’s disease (AD) is a progressive neurodegenerative disorder marked by the selective loss of neurons in various brain regions, leading to cognitive dysfunction. Neurofibrillary tangles (NFTs), composed of hyperphosphorylated tau protein accumulate within neuronal cells leading to cell death. The development of effective treatments for neurodegenerative diseases (NDs) remains a significant challenge, with neuroprotection emerging as a promising therapeutic strategy to slow disease progression and preserve cognitive function.

Objective: This study aims to investigate the potential neuroprotective effect of Gardenia jasminoides fruit extract, which contains geniposide, a biologically active compound known for its neuroprotective properties, in mitigating Tau hyperphosphorylation in …

Branching Corals Modulate Their Hydrodynamic Environment, Enhancing Capture Rates Of Evasive Prey, Kelsey Daniels

USF Tampa Graduate Theses and Dissertations

Reef-building corals obtain nutrients through two primary means: autotrophy (via mutualism with photosynthetic zooxanthellae) and heterotrophy (via feeding). The latter form of resource acquisition is understudied and many aspects of predator-prey interactions between coral and naturally occurring prey species remain unexplored. In this thesis, I elucidate the role of branching coral morphology in shaping the colony’s local hydrodynamic environment and describe how this environment impacts prey capture, particularly among evasive and hydrodynamically sensitive, but highly abundant prey such as copepods. I used acrylic cylinders of three sizes in aquaria to model the structure of coral branches across the range of …

Using Stomach Content Analysis To Determine The Trophic Ecology Of Omosudis Lowii, A Deep-Sea Predator In The Gulf Of Mexico, Claire De Noyo

USF Tampa Graduate Theses and Dissertations

Little is known about the feeding dynamics of deep-sea communities, despite their significant ecological importance. Deep-pelagic communities contribute to marine biodiversity and ecosystem stability while directly offsetting climate change via carbon sequestration. Omosudis lowii is an understudied, deep-sea fish found in temperate and tropical waters worldwide, including the Gulf of Mexico. Omosudis lowii have large jaws and distensible stomachs, allowing them to capture prey items larger than their body size. The present seminal study used stomach content analysis to quantitatively describe the O. lowii trophic ecology, providing baseline data regarding diet composition, feeding selectivity, and factors that impact prey selection. …

Identification Of Host Nuclear Factors Exploited By Kshv Lana To Maintain Latency, Anandita Ghosh

USF Tampa Graduate Theses and Dissertations

Kaposi’s Sarcoma Associated Herpes Virus (KSHV or HHV-8) is a γ-herpesvirus implicated in multiple lymphoproliferative human cancers and immune dysregulatory diseases. Like other herpesviruses, KSHV has a biphasic mode of infection primarily orchestrated by two viral proteins: the Latency-Associated Nuclear Antigen (LANA), which promotes viral persistence through latency, and the Replication and Transcription Activator (RTA), which triggers lytic reactivation. During latency, the KSHV genome exists as a highly heterochromatinized, extrachromosomal episome in the nucleus, which can be reverted to euchromatin in response to certain stimuli, like stress. KSHV has evolved to exploit numerous host proteins to orchestrate its lifecycle, especially …

The Exaggerated Blood Pressure Response To Activities Of Daily Living And Exercise Is Dependent Upon The Fitness Of People With Hypertension, Joseph D. Larsen

Theses and Dissertations

Purpose: Determine if the previously reported exaggerated systolic blood pressure response to daily activity and moderate exercise amongst hypertensive adults could be accounted for by differences in fitness. Methods: 30 normotensives adults (15 low fitness control (LFC) and 15 high fitness control (HFC)) and 15 unmedicated adults with ambulatory hypertension (HTN) participated in this study. Participants completed 24-hour ambulatory blood pressure monitoring (ABPM). Lactate Threshold (LT) and estimated ����̇O2max were determined for each participant. A week later, blood pressure was measured while participants completed 5-minute bouts of treadmill exercise at fixed absolute intensities (2.5 mph 0% and 12% grade) and …

Photo-Crosslinked Persistent Micelle Templates With Near Universal Solvent Compatibility, Coby S. Collins, Mengxue Zhang, Cj Strurgill, Christian X. Ruff, Bryce Melton, Morgan Stefik

Faculty Publications

The controlled preparation of porous nanomaterials from block polymer templates is important for diverse applications from energy devices to optical coatings and sorbents. The architectural dimensions determine the overall performance for many of these applications, however the independent control of these dimensions has remained limited to a narrow range of solvent conditions for kinetically trapped (“persistent”) micelle templates. Polymers with a photo-reactive chemistry are shown to enable cross-linked micelle templates that universally remain persistent under diverse solvent conditions. Specifically, poly(poly(ethylene glycol methacrylate))-b-poly(butyl methacrylate-co-coumarin methacrylate) (OBC) was prepared by RAFT polymerization and the resulting micelles were crosslinked …

Computational Advances For Single-Cell Proteomics, Hannah Boekweg

Theses and Dissertations

The overarching goal of biologists is to characterize biological systems. When the workings of a system are understood, they can be fixed, broken, preserved, mimicked, and even replicated. The field of proteomics seeks to characterize systems through the lens of proteins. Proteins are referred to as the 'workhorse' of the cell to illustrate that they perform most functional tasks within the cell. These tasks are highly varied, including enzymatic activity, structural support, transport, storage, cell signaling, gene regulation, immune response, and more. Proteomics has been instrumental in new biological discoveries by characterizing the functional state of cells. A new area …

Tomato Seedling Performance In Five Commercial Organic Growing Media With And Without Supplemental Fertilizer, Elizabeth Maynard, Wenjing Guan, Petrus Langenhoven, Lori A. Hoagland

Department of Horticulture & Landscape Architecture Faculty Publications

Commercially available growing media permitted for use in certified organic production differ substantially in quantity and availability of essential macronutrients. Nutrient availability from fertilizers applied during production will depend on chemical, biological, and physical characteristics of both media and fertilizers; therefore, best practices for fertilizing seedlings are likely to differ. This study evaluated tomato (Solanum lycopersicum L.) emergence, seedling growth, and posttransplant performance when grown in five organic growing media with and without supplemental fertilizer during transplant production in three diverse locations. A 5N–0.88P–3.32K–3Ca (5–2–4) dry fertilizer was tested in all media, and a liquid 3N–0.88P–2.49K (3–2–3) fertilizer was …

Science Communication Through Cool Critters: An Informal Learning Experience For Youth, Jamila L. Bajelan, Mary J. Harner

Transactions of the Nebraska Academy of Sciences and Affiliated Societies

There are numerous ways to communicate science and assess the effectiveness of that communication. One approach focuses on increasing awareness, enjoyment, interest, opinion, and/or understanding of science. For this study, we created an informal learning experience for youth to communicate the science of animals in Nebraska. We developed Cool Critters, a weeklong summer camp for elementary school-aged students, to increase participants' enjoyment, interest, and opinion of Nebraska animals. In 2023, Cool Critters was led in three educational settings in central Nebraska and reached sixty-six participants. Surveys were used to determine the camp's success in effective science communication and whether …

Global Mitophagy Inhibition Via Bnip3 Ablation Is Not Sufficient To Alleviate Skeletal Muscle Impairments In Male And Female Tumor-Bearing Mice, Francielly Morena, Ana Regina Cabrera, Toby L. Chambers, Pietier J. Koopmans, Seongkyun Lim, Stavroula Tsitanou, Sabin Khadgi, Calvin Peterson, Eleanor R. Schrems, Ruqaiza Muhyudin, Sepideh Shakeri, Kevin Zhao, Devan Mishra, Tyrone Washington, Kevin A. Murach, Nicholas P. Greene

Health, Human Performance and Recreation Faculty Publications and Presentations

Cancer cachexia (CC) is marked by severe skeletal muscle loss and dysfunction, associated with mitochondrial degeneration. Our previous studies showed induction of the mitophagy marker BNIP3 3-wk post-Lewis lung carcinoma (LLC) induction. We hypothesize that excessive mitophagy contributes to muscle wasting in CC. To test this, we used a Bnip3 knockout (KO) mouse model with LLC-induced CC to assess its impact on muscle outcomes. Eight-wk-old male and female mice were injected with 1 x 10(6) LLC cells or PBS (sham controls). After 4 wk, we assessed muscle function through dorsiflexor electrophysiology, muscle protein synthesis via deuterium oxide labeling, and mitochondrial …

Potential Epigenetic Impacts Of Phytochemicals On Ruminant Health And Production: Connecting Lines Of Evidence, Sebastian P. Schreiber, Juan Villalba, Mirella L. Meyer-Ficca

Wildland Resources Student Research

The once mysterious “dark matter of nutrition”, comprising countless plant derived secondary compounds, also known as phytochemicals, is now understood to have significant and wide-ranging effects on consumers, including myriad health benefits in humans and livestock. The selective consumption of phytochemically rich and diverse plants, in appropriate doses, by ruminants represents an adaptive means of therapeutic and prophylactic self-medication. Due to their chemical structure, phytochemicals have long been recognized as antioxidants. However, the mechanisms that underlie numerous additional phytochemical-based health benefits are generally less understood. These effects (i.e., anti-inflammatory, immunomodulatory, and anticarcinogenic effects) are likely related to epigenetic processes. Evidence …

Dosimetric Advantage Of Scanning Beam Proton Therapy In Gynecologic Patients Receiving Adjuvant Radiotherapy, Rachel B Ger, Jarrod M Lentz, Joshua S Niedzielski, Sujay A Vora, Martin Bues, Danairis Hernandez Morales, Justin D Anderson, Christopher J Kutyreff, Christie A Schulz, Pedro R Lara, Ana K Ridgway, Pamela R Lemish, Justin D Gagneur, Aman Anand

Faculty, Staff and Student Publications

Background/Objectives: Adjuvant radiation for gynecologic malignancies often exposes organs at risk (OARs), such as the bone marrow, bowel, rectum, and bladder, to radiation, leading to toxicities that impact treatment tolerance and patient quality of life. Scanning proton beam therapy, particularly with Individual Field Simultaneous Optimization (IFSO), may offer dosimetric and biological advantages over volumetric modulated arc therapy (VMAT). This study evaluates the clinical impact of IFSO-based proton planning in post-operative gynecologic cancer patients.

Materials and Methods: Fourteen patients receiving adjuvant proton therapy to 45 Gy in 25 fractions were retrospectively analyzed. Comparison VMAT plans were generated on the same datasets. …

Identification Of Hypoxia-Inducible Factor Hif1a As A Therapeutic Target During Sars-Cov-2-Associated Lung Injury, Bentley Bobrow, Samuel D Luber, Paul Potnuru, Katherine Figarella, Jieun Kim, Yanyu Wang, In Hyuk Bang, David Robinson, Paulina B Sergot, Steven K Burke, Tingting Mills, Constanza De Dios, Robert Suchting, George W Williams, Adit A Ginde, Yafen Liang, Hongfang Liu, Charles Green, Marie-Francoise Doursout, Alparslan Turan, Daniel I Sessler, Xiaoyi Yuan, Holger K Eltzschig

Faculty, Staff and Student Publications

Hypoxia-inducible factors (HIFs) promote lung protection and pathogen eradication during acute lung injury. We therefore tested the theory that pharmacologic stabilization of HIFs dampens lung injury during SARS-CoV-2 pneumonia. Initial studies in murine SARS-CoV-2 models showed improved outcomes after treatment with the FDA-approved HIF-stabilizer vadadustat. Subsequent studies in genetic models implicated alveolar-expressed Hif1a in mediating lung protection. Therefore, we performed a randomized, double-blinded, multicenter phase 2 trial in patients admitted for SARS-CoV-2 infection and concomitant hypoxia (SpO2 ≤ 94%). Patients (n=448) were randomized to oral vadadustat (900 mg/day) or placebo for up to 14 days. Safety events were similar between …

Beyond Diversity Initiatives: Building A Community Of Care In Scientific Societies, Richelle L. Tanner, Kathryn Wilsterman

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

"In this Professional Biologist article, we present one such reframing that centers individualized care through structural changes. We argue that psychological and sociological motivations of belonging and social capital in the academy are funda- mental challenges contributing to the cultural problems within STEM fields and are particularly present within professional scientific societies. In support of this argument, we present survey- based data alongside significant literature from the humanities. In this reframing supported by existing organizational culture literature and our survey results, we suggest that scientific societies are uniquely positioned to promote significant cultural change within STEM by focusing on building …

Integrative Taxonomy Confirms The Synonymy Of Three Springsnail Species (Hydrobiidae: Pyrgulopsis), Kathryn E. Perez, Megan Solis, Michelle Flores, Chanté Lundskog

School of Integrative Biological & Chemical Sciences Faculty Publications

The small, spring-dwelling snails, Pyrgulopsis Call & Pilsbry, 1886, form a diverse radiation in Western North America. In this study, we examine the validity of three species of Pyrgulopsis, Pyrgulopsis pilsbryana (Baily & Baily, 1952), P. nonaria Hershler, 1998, and P. transversa Hershler, 1998. These three species of Pyrgulopsis are found primarily in Utah, with ranges extending into Idaho, Wyoming, and Nevada. They were originally diagnosed primarily on the number and position of penial glands, but subsequent examination with mitochondrial DNA data suggested they should be synonymized. Here, we incorporate additional mitochondrial and nuclear DNA data, increased geographic sampling, …

Using Lrr Standard Deviation As A Genotype Quality Control Measure And Its Downstream Effect On Cnv Calling., Adam Dunne

ORBioM (Open Research BioSciences Meeting)

Background:

Log R Ratio (LRR) is a genotype intensity measurement returned alongside routine genotype results and used to detect structural variation in the genome known as copy number variation (CNVs). Although LRR standard deviation (SD) is commonly applied as a quality control measure in CNV studies, limited research has explored its effect on genotype quality and the potential impact on CNV calling.

Methods:

A total of 720,152 genotypes were available on 716,234 cattle. Among these were 1,044 cattle that had duplicate genotype samples where one sample was considered gold standard (LRR SD < 0.3, Call Rate ≥ 0.95). Genotype concordance was calculated for all duplicates. PennCNV was used to call CNVs and concordance among CNV calls per duplicate animal was determined using the Jaccard index.

Results:

Across all 720,152 cattle samples, the mean …

Selecting For Less: How Much Methane Can We Truly Breed Away?, Sean B. Crowley

ORBioM (Open Research BioSciences Meeting)

Background

Genetic selection has potential to reduce enteric methane emissions from cattle, but a key challenge is determining how much of the genetic variation in methane traits is independent of traits already targeted in breeding programs, such as feed intake and growth.

Methods

Methane and carbon dioxide emissions were recorded in 1,700 crossbred, indoor-fed beef cattle using Greenfeed systems. Performance data included feed intake, metabolic liveweight, average daily gain, carcass weight, and ultrasound and skeletal traits. Genetic parameters and correlations among traits were estimated using animal linear mixed models to quantify the independence of methane-related genetic variation.

Results

Daily methane …

Exploiting Genomics To Advance Genetic Gain For Reproductive Traits In Sheep, Mujibur Rahman

ORBioM (Open Research BioSciences Meeting)

Poor reproductive performance has a negative impact on the profitability of sheep production systems with the number of lambs reared highlighted as a key driver of farm profitability. Nevertheless, the genetic improvement of reproductive traits in sheep has been constrained by their low heritability and pleiotropic nature. Understanding the underlying genetic architecture can improve genomic prediction estimates and enhance genetic gain.

This study will utilize large-scale genomic and phenotypic datasets to enhance genetic gain in sheep reproductive traits through four approaches: 1) to determine the effect of inbreeding depression on reproduction, 2) to identify causal variants impacting reproductive performance through …

Dna-Damaging Chemotherapy Reshapes The Immune Landscape Of The Heart, Ruijun He

Arts & Sciences Graduate Student Theses and Dissertations

Heart failure and ischemic heart disease represent the most common causes of death among cancer survivors. With the tremendous progress in last few decades that empowered early cancer diagnosis and treatment, the population of cancer survivors has been rapidly growing and by 2040, the number is projected to be 26 million. However, despite extensive studies on chemotherapies, there exists a limited understanding of how exposure to chemotherapy reagents impacts heart function in the long term. Moreover, while the roles of immune landscape in heart diseases have recently been revealed, how chemotherapy reagents influence the cardiac immune cells is poorly understood. …

Proceedings Of The Cuny Games Conference 11.0, Robert O. Duncan, Grace Axler-Diperte, Joe Bisz, Christina Boyle, Devorah Kletenik, Carolyn Stallard

Publications and Research

The CUNY Games Conference combines workshops, idea exchanges, interactive participant presentations, playtesting, and playing tabletop games into a one-day online event to promote and discuss game-based learning. The conference focuses on creative pedagogy, such as playful learning activities or games, that teachers can use in the classroom every day. The conference is online and features interactive presentations by attendees, informal idea exchange sessions, and workshops by the conference organizers.

Resist!: Sustaining Forest Carbon Sequestration And Wood Production After Insect Disturbance, Christopher M. Gough, Ben Bond-Lamberty, Robert T. Fahey, Catherine Mcguigan, Brandon C. Alveshere, Cameron Clay, Erin Darby Mcclain, Charles E. Flower, Kayla C. Mathes, Ariel Johnson, Lisa T. Haber, Kalyn Dorheim, Derek M. Johnson, Lucas E. Nave, Kerstin Niedermaier

Michigan Tech Publications

Disturbances from insect pests threaten ecologically and economically important goods and services supplied by forests, including wood production and carbon sequestration. We highlight the factors that influence these services’ resistance, a term quantifying the initial response to disturbance. Insects inflict damage through a range of mechanisms, prompting distinct plant physiological responses that scale to influence ecosystem processes and, with time, goods and services. The degree and timing of tree mortality and defoliation affect the amount of residual vegetation available to support compensatory wood production and influence carbon sequestration by changing rates of detritus-fueled decomposition. Compounding, or sequential, insect attacks may …

The Role Of Neuroscience In Mental Health Management Within The Adhd Population, Kadi Rae Smith

University Honors Theses

Neuroscience-based educational interventions play a role in the management of Attention Deficit Hyperactive Disorder (ADHD), including the regulation of ADHD-correlated depression and anxiety. Existing research shows that elevated rates of depression correspond to anxiety within the ADHD community; approximately 8.83% of the global population. Social ostracization and isolation are established contributors to depression and anxiety which are commonly comorbid with ADHD. The need exists for further investigation of the beneficial impact that an accurate, biological understanding of how the brain works has on the mental health of the ADHD population, and by extension their families, education systems, and medical industries. …

The Tumor Suppressor Hnrnpk Induces P53-Dependent Nucleolar Stress To Drive Ribosomopathies, Pedro Aguilar-Garrido, María Velasco-Estévez, Miguel Ángel Navarro-Aguadero, Álvaro Otero-Sobrino, Marta Ibáñez-Navarro, Miguel Ángel Marugal, María Hernández-Sánchez, Prerna Malaney, Ashley Rodriguez, Oscar Benitez, Xiaroui Zhang, Marisa Jl Aitken, Alejandra Ortiz-Ruiz, Diego Megías, Manuel Pérez, Gadea Mata, Jesús Gomez, Miguel Lafarga, Orlando Domínguez, Osvaldo Graña-Castro, Eduardo Caleiras, Pilar Ximénez-Embun, Marta Isasa, Paloma Jimena De Andres, Sandra Rodríguez-Perales, Raúl Torres-Ruiz, Enrique Revilla, Rosa María García-Martín, Daniel Azorín, Josune Zubicaray, Julián Sevilla, Oleksandra Sirozh, Vanesa Lafarga, Joaquín Martínez-López, Sean M Post, Miguel Gallardo

Faculty, Staff and Student Publications

The nucleolus is a membraneless organelle and an excellent stress sensor. Any changes in its architecture or composition lead to nucleolar stress, resulting in cell cycle arrest and interruption of ribosomal activity, critical factors in aging and cancer. In this study, we identified and described the pivotal role of the RNA-binding protein HNRNPK in ribosome and nucleolar dynamics. We developed an in vitro model of endogenous HNRNPK overexpression and an in vivo mouse model of ubiquitous HNRNPK overexpression. These models showed disruptions in translation as the HNRNPK overexpression caused alterations in the nucleolar structure, resulting in p53-dependent nucleolar stress, cell …

Technical And Clinical Validity Of Assessing Measurable Residual Disease By Multicolor Flow Cytometry In An Unselected Acute Myeloid Leukemia Patient Cohort, Sa A Wang, Shaoying Li, Wei Wang, Jie Xu, Beenu Thakral, Shimin Hu, Chi Young Ok, Fuli Jia, Jeffrey L Jorgensen, L Jeffrey Medeiros, Farhad Ravandi, Nicholas J Short, Sanam Loghavi

Faculty, Staff and Student Publications

Context.—: Following the validation of a multicolor flow cytometry (MFC) assay for measurable residual disease (MRD) in acute myeloid leukemia (AML), this study examines its clinical applicability.

Objective.—: To evaluate the practicality and performance of MFC-based MRD detection in AML.

Design.—: Prospectively assessed AML MRD MFC in unselected AML patients achieving morphologic remission with follow-up studies, molecular genetics, and survival data.

Results.—: Among 379 patient bone marrow samples in this cohort, an interpretable result was obtained in 359 (95%). A total of 57 of the 359 cases (16%) were positive for MRD, and the most frequently observed immunophenotype was CD34+CD117+ …

Vitamin D And Cognition: Demographic Disparities In Memory Recall And Word Intrusion In A Multiethnic Cohort, Juan Lopez-Alvarenga, Isabel Omaña-Guzmán, Oscar Rosas-Carrasco, Jose E. Cavazos, Michael C. Mahaney, Gladys E. Maestre

School of Medicine Publications

Background

Vitamin D3 is essential for calcium metabolism and exerts pleiotropic effects, including neuroprotective activities in cognition. Its insufficiency has been linked to dementia, Alzheimer's disease, and cognitive impairments. The association between vitamin D3 and particular cognitive functions, including memory recall and word intrusion, remains imprecise, particularly among diverse ethnic and socioeconomic groups.

Objective

To examine the relationship between vitamin D3 levels with memory recall and word intrusion in individuals aged 60 and above, emphasizing demographic differences.

Methods

Data was collected from 2759 individuals in the NHANES 2011–2014 surveys. Cognitive performance was evaluated with the CERAD Word Learning, Animal Fluency, …