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Characterizing Gsk3Β Interaction And Kinetics Via Isothermal Titration Calorimetry, W A Bhagya De Silva

UNLV Theses, Dissertations, Professional Papers, and Capstones

Glycogen synthase kinase 3β (GSK3β) is a multifunctional serine/threonine kinase involved in several key signaling pathways, including glycogen metabolism, WNT/β-catenin, and Hedgehog signaling. Hyperactivity of GSK3β has been linked to Alzheimer’s disease, bipolar disorder, type II diabetes, and some cancers. Therefore, GSK3β is of interest as a target for therapeutics. Lithium ion (Li+) is a classical inhibitor of GSK3β. Structurally similar beryllium ion (Be2+) is ~1000-fold more potent. Lithium and beryllium have demonstrated pathway-specific and cell type-specific inhibition of GSK3β, prompting an investigation into the inhibitory mechanisms and thermodynamic characteristics of the metal-enzyme interaction.Isothermal titration calorimetry (ITC) is a label-free …

High Fat Diet & Social Isolation: Interactive Effects On Pain, Cognition, & Neuroinflammation, Ian M. Campuzano

Research Psychology Theses

Prior research has established a role for both social isolation and exposure to high fat Western diets in altering a range of behaviors from reduced memory performance to increased depression-like behaviors. The present study scrutinizes the interplay among these variables during the peri-adolescent developmental phase, utilizing Long-Evans rats as the experimental model. Our overarching hypothesis is that rats exposed to either social isolation, a high-fat diet, or both will result in heightened pain sensitivity, diminished cognitive flexibility, and increased neuroinflammatory responses within brain regions implicated in sociability, cognition, memory, and pain processing. Behavioral flexibility will be assessed using a maze-based …

Gpr171 Modulates Mood, Consummatory Behaviors, And Morphine Antinociception In A Sexually Dimorphic Pattern In Mice, Megan C. Raddatz

All Graduate Theses and Dissertations, Fall 2023 to Present

Receptors in the brain influence everything from complex behaviors related to mood, all the way to simple physiological functions like the way a person moves. Receptors are activated or inactivated by chemicals or hormones that the body produces or that are created to mimic the body’s natural chemicals. Of the hundreds of receptors in the brain, GPR171 is particularly interesting because new drugs have been created to activate or block the receptor, and are being proposed for the treatment of different disorders, particularly disorders related to pain. GPR171 has been shown to affect pain behaviors, eating, and mood-related behaviors, but …

Socially Valued, Ecologically In Decline: Place Attachment Influences Support For Management Actions In A Quaking Aspen Forest Impacted By Recreation, Soil Contamination, And Ungulates, Georgie Corkery

All Graduate Theses and Dissertations, Fall 2023 to Present

Quaking aspen (Populus tremuloides Michx.) spark strong emotional attachments among many people, yet they are ecologically in decline across North America. Aspen landscapes are also popular outdoor recreation destinations. Site-specific strategies are required for both managing for healthy aspen landscapes and enhancing the outdoor recreation experience. Our study investigated a highly-recreated and ecologically declining aspen forest in Summit County, Utah facing population decline. There were two distinct phases of data collection. First, we evaluated the condition of the aspen and identified potential management actions that could improve the condition of the aspen. Second, we surveyed people who visited the site …

Wired For Success: A Neurobiological Approach To Improving The Academic Outcomes Of Middle School Boys, Allison Emery

University Honors Theses

Male accomplishment in scholastic settings has been declining globally for the last decade, leading to long-term consequences for boys and men. Possible neurobiological underpinnings of the gender gap in education are discussed in this literature review. While male and female differences have, in the past, largely been considered socialized, recent science also confirms neurobiological variances between male and female development. These distinctions are assessed for their potential ties to the decline in male academic success, and are correlated to classroom practices, strategies and interventions meant to increase boys' engagement and performance.

Genomic Data Science Approaches For Understanding Human Diseases, Snehal Shah

All Dissertations

The intricate interplay of genetic predisposition, environmental influences, and lifestyle acts as the multifactorial landscape of diseases. Understanding this complexity presents a significant challenge. Molecular insights into disease mechanisms, particularly the interactions of DNA, RNA, and proteins with environmental and lifestyle factors, have revolutionized disease diagnosis, prognosis, and treatment. High-throughput technologies, such as next-generation sequencing, generate large amounts of molecular data, holding a wealth of knowledge. These datasets unveil the roles of genes and their interactions with various factors through analysis, shedding light on previously unknown molecular mechanisms underlying disease pathogenesis. Furthermore, they facilitate the discovery of biomarkers crucial for …

Advancing Methods For Studying The Neuroscience Of Sex Variability And Diversity, Vic Shao-Chih Chiang

Graduate Doctoral Dissertations

Sex variability and diversity are pervasive across taxa and genera. Society is heavily structured around sex/gender conceptualizations, and neuroscience research actively shapes these constructs. This dissertation adopts frameworks that challenge ideologies perpetuating sex/gender injustices and developed neuroscience-related methodologies that could advance the conceptualisations of sex. First, I studied B6D2F1 mice which exhibit sexual behaviour months after castration. This challenged the dogma that sexual behaviour is dependent on gonadal steroid hormones. I enhanced the behavioural analysis of these mice using deep learning techniques. Second, applying similar deep learning methods, I refined the behavioural analysis of Astatotilapia burtoni cichlids, which exhibit sexual …

Consumer Willingness To Pay For Food Products Displaying A Black-Owned Production Label, Logan Gage Moss

Graduate Theses and Dissertations

Black and African Americans have faced historic underrepresentation in the agri-food system (Horst & Marion, 2019). Systemic inequities have hindered food entrepreneurs and economic growth within this community. Recent diversity, equity, and inclusion (DEI) initiatives highlight societal concerns about these imbalances (Darity, 2024). Some consumers aim to address this underrepresentation by purchasing products from Black entrepreneurs, but this requires effective communication, which can increase marketing expenses and risks for producers. However, highlighting Black-owned food products may enhance outcomes for this marginalized community. Credence attributes like Black ownership cannot be evaluated even after consumption, unlike traits such as taste and quality. …

Benchmarking Lung Cancer Screening Programmes With Adaptive Screening Frequency Against The Optimal Screening Schedules Derived From The Engage Framework: A Comparative Microsimulation Study, Mehdi Hemmati, Sayaka Ishizawa, Rafael Meza, Edwin Ostrin, Samir M Hanash, Mara Antonoff, Andrew J Schaefer, Martin C Tammemägi, Iakovos Toumazis

Faculty, Staff and Student Publications

Background: Lung cancer screening recommendations employ annual frequency for eligible individuals, despite evidence that it may not be universally optimal. The impact of imposing a structure on the screening frequency remains unknown. The ENGAGE framework, a validated framework that offers fully dynamic, analytically optimal, personalised lung cancer screening recommendations, could be used to assess the impact of screening structure on the effectiveness and efficiency of lung cancer screening.

Methods: In this comparative microsimulation study, we benchmarked alternative clinically relevant structured lung cancer screening programmes employing a fixed (annual or biennial) or adaptive (start with annual/biennial screening and then switch to …

A Visualization And Radiation Treatment Plan Quality Scoring Method For Triage In A Population-Based Context, Alexandra O Leone, Abdallah S R Mohamed, Clifton D Fuller, Christine B Peterson, Adam S Garden, Anna Lee, Lauren L Mayo, Amy C Moreno, Jay P Reddy, Karen Hoffman, Joshua S Niedzielski, Laurence E Court, Thomas J Whitaker

Faculty, Staff and Student Publications

PURPOSE: Our purpose was to develop a clinically intuitive and easily understandable scoring method using statistical metrics to visually determine the quality of a radiation treatment plan.

METHODS AND MATERIALS: Data from 111 patients with head and neck cancer were used to establish a percentile-based scoring system for treatment plan quality evaluation on both a plan-by-plan and objective-by-objective basis. The percentile scores for each clinical objective and the overall treatment plan score were then visualized using a daisy plot. To validate our scoring method, 6 physicians were recruited to assess 60 plans, each using a scoring table consisting of a …

Disparities In Exposure To Tobacco On Television Or Streaming Platforms, Henry K Onyeaka, Onyema G Chido-Amajuoyi, Itunu Sokale, Kobi V Ajayi, A Eden Evins, Hermioni L Amonoo, Sanjay Shete

Faculty, Staff and Student Publications

Importance: With the rise in popularity of streaming platforms concerns about exposure to tobacco advertising and promotion have emerged. While tobacco marketing and promotion through traditional television (TV) media channels has been extensively studied, less is known about exposure to tobacco through TV or streaming platforms and its associated factors.

Objective: To examine the prevalence and factors associated with exposure to tobacco products advertised, marketed, or promoted on TV or streaming platforms among US adults.

Design, setting, and participants: This cross-sectional study used data from the National Cancer Institute's Health Information National Trends Survey (HINTS 6), conducted from March 7 …

Simulation-Based Caregiving Skills Training For Family Members Of High-Grade Glioma Patients, Meagan Whisenant, Shiao-Pei Weathers, Yisheng Li, Ellen Aldrich, Kristin Ownby, Jessica Thomas, An Ngo-Huang, Eduardo Bruera, Kathrin Milbury

Faculty, Staff and Student Publications

BACKGROUND: Because family caregivers of patients with a high-grade glioma experience high levels of distress and feel unprepared to perform the complex caregiving tasks associated with the disease and its treatment, we pilot-tested a caregiving skills intervention that integrates hands-on caregiving with coping skill training.

METHODS: In this single-arm trial, caregivers participated in a 4-session research nurse-led intervention involving simulation-based caregiving skills training at the hospital and psychoeducation delivered via videoconference. We collected measures of patients' and caregivers' psychological symptoms; caregivers' caregiving self-efficacy and role adjustment; and patients' cancer-related symptoms (MDASI) at baseline and again postintervention. We tracked feasibility data. …

A Qualitative Exploration To Inform An Oral Health Training For Disability Care Workers In Burkina Faso, Ave Põld, Dan Filwendé Kientega, Jocelyne Valérie Garé, Michael Lorenz

Faculty, Staff and Student Publications

AIM: To explore enablers and barriers to the creation of an oral health training for care workers at specialized centres for children with disabilities in Ouagadougou.

DESIGN: This was a formative study informed by the Theoretical Domains Framework using qualitative methods.

METHODS: Qualitative observations and 14 semi-structured interviews were conducted with care workers from six specialized centres for disability.

RESULTS: Carer workers emphasized that a successful oral health training must account for available resources and competencies in each specific centre. Part of the training must be dedicated to oral hygiene targeted for people with disabilities and provide knowledge about risk …

A Qualitative Narrative Study Of Rescue And Recovery Workers Responding To The Terrorist Bombing Of Oklahoma City's Murrah Building, E Whitney Pollio, Jennifer Wang, Edward Randle, David E Pollio, Carol S North

Faculty, Staff and Student Publications

OBJECTIVE: Much of disaster mental health research uses quantitative methods, focusing on numerical prevalence, services, and outcomes.

METHODS: Qualitative methods can provide more detailed, rich, and spontaneous insights into personal disaster experiences, yielding important insights beyond deductive methods. This large-scale qualitative narrative study examined experiences of 181 Oklahoma City bombing rescue/recovery workers.

RESULTS: Thematic narrative content of the bombing experience arose from personal accounts of the bomb blast by rescue/recovery workers proceeding chronologically from initial awareness and deployment to harrowing onsite search and rescue/recovery missions to the aftermath with reflections on the bombing.

CONCLUSIONS: Beyond disaster recovery/rescue worker stories published …

The Effects Of Non-Invasive Brain Stimulation On Excitability Of The Primary Motor Cortices And Complex Motor Task Learning, Erik Woodruff Wilkins

UNLV Theses, Dissertations, Professional Papers, and Capstones

Transcranial direct current stimulation (tDCS) and transcranial alternating current stimulation (tACS) are promising non-invasive brain stimulation techniques, particularly for clinical applications involving altered or impaired motor function, such as in stroke and Parkinson’s disease. Although tDCS and tACS are different modalities, both forms of stimulation operate on the same principle - weak electrical currents are delivered through electrodes placed on the scalp in specific configurations known as “montages”. The most commonly used montage for targeting the primary motor cortex (M1) is the M1-SO configuration. This montage leverages the established observation that cortical regions directly beneath the anode generally increase in …

Preschool Executive Control And Adolescent Dietary Outcomes: Highlighting The Role Of Context, Marla Lopez

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Research has identified executive control (EC) as an important predictor of dietary outcomes among youth. Executive control in preschool age has been shown to predict important outcomes across development for youth, indicating that good, foundational EC abilities are associated with successful development in later life. Dietary outcomes across adolescence, an important transitional period for establishing healthy eating behaviors, are understudied. Therefore, the current study sought to link two critical developmental periods, examining how preschool EC is associated with dietary trajectories across adolescence. Further, the study aimed to identify how contextual factors, specifically indices of socio-economic status and the home and …

Emotional Content In Working Memory Processes: An Fmri Study., Manal Zafar

Electronic Theses and Dissertations

Working memory (WM) is a dynamic system that processes and manipulates both goal-relevant and goal-irrelevant information, integral to higher-level cognition and executive control (Fujii, 2001; Nyberg & Erikkson, 2016). The processing and manipulation of representations is modulated by the affective nature of the information (Pessoa, 2009) such that negative information can either impair or enhance WM processing. WM impairments are seen commonly across clinical disorders such as depression, anxiety, PTSD and schizophrenia (Levens & Gotlib, 2010; Joorman et a., 2011; Brodziak et al., 2015). Specifically, negative information is known to have differential processing in WM compared to neutral and positive …

Atypical Responses To Sensory Stimuli Or Eating Environments In Children With Autism Spectrum Disorder, Lydia Rockers

Graduate Theses and Dissertations

Children with autism spectrum disorder (ASD) often encounter challenges in processing sensory inputs. Children with ASD may experience feeding difficulties and over- or under- stimulation during food-related activities such as eating or shopping. These have impacts on the health and well-being of individuals with ASD in childhood and throughout their lives. Further research is needed to address the impact of ambient sound levels in a retail or restaurant setting. The main objective of this thesis was to address these gaps by identifying responses to various environmental and food related stimuli. The findings of this research addressed differences in responses between …

Spectral Sensitivity Of Circadian Entrainment In Araneoid And Non-Araneoid Spiders, Craig Campbell

Electronic Theses and Dissertations

Artificial lighting at night is a growing worldwide phenomenon that can negatively impact flora and fauna in the surrounding area. With new technology, there is now opportunity for change: newer LEDs can emit specific wavelengths of light that may be less ecologically damaging. In this study, we aim to describe the circadian spectral sensitivity of two phylogenetically distant urban spider species with similar life histories, the Araneoid Parasteatoda tepidariorum and non-Araneoid Pholcus manueli, to determine differences between them. We exposed these spiders to progressively dimmer levels of white, blue, green, or red light. Despite evidence that spiders lack visual …

Effects Of Interrupting The Scotophase Period On Bone Health And Performance Of Laying Hens, Alexis J. Clark

All Theses

This thesis aimed to evaluate the effects of interrupting the daily scotophase period on bone health and production in laying hens. Modern commercial laying hens are prone to high rates of bone demineralization due to the constant mobilization of calcium to withstand daily eggshell calcification mechanisms. The industry light standard for laying hens is 16 hours of continuous light (photophase) and 8 hours of continuous darkness (scotophase) daily. During scotophase, physical activity is reduced due to instinctive perching behaviors, and thus, feeding behaviors are halted. However, physiological processes, including digestion, excretion, and reproduction, remain active during scotophase.

Most eggshell formation …

Challenges In Lncrna Biology: Views And Opinions, Donald A Adjeroh, Xiaobo Zhou, Alexandre Rossi Paschoal, Nadya Dimitrova, Ekaterina G Derevyanchuk, Tatiana P Shkurat, Jeffrey A Loeb, Ivan Martinez, Leonard Lipovich

Faculty, Staff and Student Publications

This is a mini-review capturing the views and opinions of selected participants at the 2021 IEEE BIBM 3rd Annual LncRNA Workshop, held in Dubai, UAE. The views and opinions are expressed on five broad themes related to problems in lncRNA, namely, challenges in the computational analysis of lncRNAs, lncRNAs and cancer, lncRNAs in sports, lncRNAs and COVID-19, and lncRNAs in human brain activity.

Automatic Uncovering Of Patient Primary Concerns In Portal Messages Using A Fusion Framework Of Pretrained Language Modelsautomatic Uncovering Of Patient Primary Concerns In Portal Messages Using A Fusion Framework Of Pretrained Language Models, Yang Ren, Yuqi Wu, Jungwei W Fan, Aditya Khurana, Sunyang Fu, Dezhi Wu, Hongfang Liu, Ming Huang

Faculty, Staff and Student Publications

OBJECTIVES: The surge in patient portal messages (PPMs) with increasing needs and workloads for efficient PPM triage in healthcare settings has spurred the exploration of AI-driven solutions to streamline the healthcare workflow processes, ensuring timely responses to patients to satisfy their healthcare needs. However, there has been less focus on isolating and understanding patient primary concerns in PPMs-a practice which holds the potential to yield more nuanced insights and enhances the quality of healthcare delivery and patient-centered care.

MATERIALS AND METHODS: We propose a fusion framework to leverage pretrained language models (LMs) with different language advantages via a Convolution Neural …

Utilising Discriminant Function Analysis (Dfa) For Classifying Osteoarthritis (Oa) Patients And Volunteers Based On Biomarker Concentration, Laura Jane Coleman, John L Byrne, Stuart Edwards, Rosemary O'Hara

Faculty, Staff and Student Publications

Osteoarthritis (OA) is a degenerative joint disease characterised by the breakdown of cartilage, causing pain, stiffness, and limited movement. Early diagnosis is crucial for effective management but remains challenging due to non-specific early symptoms. This study explores the application of Discriminant Function Analysis (DFA) to classify OA patients and healthy volunteers based on biomarker concentrations of Interleukin-6 (IL-6), Tumour necrosis factor-alpha (TNF-α), and Myeloperoxidase (MPO). DFA was employed to analyse biomarker data from 86 participants (58 patients, 28 volunteers) to evaluate the discriminatory power of these biomarkers in predicting OA. Significant differences were observed in MPO and TNF-α levels between …

Social Entrepreneurship In Sustainable Development: Strengthening The Environmental Perspective, Muhammad Iqbal Rezky, Rasto Rasto

Journal of Environmental Science and Sustainable Development

Social entrepreneurship and sustainable development are closely related concepts. Social entrepreneurship applies entrepreneurial attributes with a focus on solving major social issues. The relationship between the two demonstrates the contribution of social entrepreneurship in addressing social and environmental problems, reducing poverty, and fostering sustainable economic growth. This research employs a bibliometric approach to analyze trends and patterns in 181 relevant articles published between 2015 and 2023. Findings indicate significant growth in research between 2019 and 2021. Countries and institutions such as Spain, the United States, and Universitat de Valencia stand out in these publications. The bibliometric analysis also reveals influential …

The Role Of Destination Image And Risk Perception On Interest In Revisiting Disaster-Prone Coastal Destinations, Fitri Rahmafitria, Heri Puspito Diyah Setiyorini, Purna Hindayani, Andrian Ramadhan

Journal of Environmental Science and Sustainable Development

The current trend in nature tourism has led to increased tourist visits to coastal areas, including destinations prone to disasters and previously exposed to tsunamis. This research, which aims to analyze the variables influencing tourists' desire to revisit disaster-prone areas, addresses a crucial and timely topic in tourism and disaster management. The research utilized a quantitative approach through a questionnaire-based survey of domestic tourists visiting the Pangandaran Beach Area in West Java, known for its susceptibility to tsunami disasters. The study involved 307 respondents who completed questionnaires between June and July 2023. Data analysis employed Partial Least Square – Structural …

Species Vary In Within-Species Variability, Jennifer Vonk

Animal Sentience

Owens et al. (2024) add to the growing voices stressing the importance of considering individual differences in animal welfare and conservation. I advocate for an additional emphasis on the importance of between species comparisons of the degree of individual variation in cognition and emotion within species. A better understanding of the factors predicting within-species variability will help conservationists target their efforts. Additionally, I caution against invoking circularity in using “behavioural traits” to predict related behaviours.

Social Marketing And The Challenges Of Participant Recruitment, Tom Baranowski, Teresia M O'Connor, John A Jimenez-Garcia, Kavita Radhakrishnan, Elva M Arrendondo, Debbe Thompson

Children’s Nutrition Research Center Staff Publications

Recruitment of research participants often has not obtained a desired sample size, thereby becoming a major problem inhibiting investigators' ability to adequately test the specified hypotheses. Social marketing with its four Ps, originally developed by the business world to sell products and adapted for public health initiatives, is proposed as a dynamic comprehensive conceptual framework to apply marketing principles and practices to enhance participant recruitment. Applying a social marketing approach to research recruitment would require initial investigation to understand the motivations of the target audience in regard to research participation, and continued research throughout the main project to adapt the …

A Shield-Bearer’S Tale: Elucidating The Roles Of Estradiol And Choline In Mitigating Chemotherapy-Related Cognitive Impairment, Robert W. Botelho Jr.

USF Tampa Graduate Theses and Dissertations

Chemotherapy-Related Cognitive Impairments (CRCIs) are significant consequences of chemotherapy that affect a substantial portion of breast cancer survivors, potentially persisting long after treatment cessation. This series of studies investigated the neuroprotective roles of estradiol and choline against CRCIs induced by cyclophosphamide and doxorubicin (CYP/DOX), commonly known as Adriamycin/Cytoxan (AC) chemotherapy. Utilizing the MMTV-PyVT mouse model of breast cancer, this body of work spans several experimental studies, the first of which evaluates the impact of dietary choline supplementation on spatial memory and cholinergic function in tumor-bearing mice undergoing chemotherapy. The subsequent study tests whether impaired ovarian function and resulting reductions in …

Takiri Ake Nga Haeata: Lighting A Path To Increased Indigenous Leadership In Aotearoa, Kaylee Ihimaera

Theses and Dissertations

This thesis addresses the underrepresentation of indigenous Māori people in leadership positions and pursues the need to develop a Māori leadership framework aimed at empowering the next generation of indigenous people. Several frameworks have attempted to exhibit Māori leadership dynamics, each offering unique insights into the cultural and relational aspects of Māori leadership. However, existing frameworks often exhibit limitations in fully encapsulating the breadth of Māori leadership values and practices, necessitating the development of a comprehensive Māori leadership model independent of Western frameworks. Through an extensive review of existing literature and engaging with Māori experts, I synthesized key insights to …

The Potential Influence Of Immune Modulatory Molecules (Tgf-Βiii And Ctla-4) In Pathogenicity Of Pcos, Mustafa Riyadh Abdullah, Hazima Mossa Alabassi

Karbala International Journal of Modern Science

Polycystic ovary syndrome (PCOS) is characterised by chronic anovulation, hyperandrogenism, polycystic ovaries, and immunological dysregulation. Immune homeostasis and inflammation management require immune functions. T-regulatory cells (Tregs) regulate PCOS's immune system, inflammation, insulin resistance, ovarian function, and homeostasis. Transforming growth factor βIII (TGF-βIII) and Cytotoxic-T-lymphocyte-associated-protein-4 (CTLA-4) receptors on the surfaces of Treg cells are important for controlling the immune system. The study included 68 PCOS patients and 22 non-PCOS women controls aged 20–45. The waist-hip ratio (WHR) determines obesity. We evaluated serum levels and gene expression of TGF-βIII and CTLA-4 via ELISA and real-time PCR. Women with PCOS had significantly higher …