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Identification Of Carbohydrate Gene Clusters Obtained From In Vitro Fermentations As Predictive Biomarkers Of Prebiotic Responses, Car Reen Kok, Devin J. Rose, Juan Cui, Lisa Whisenhunt, Robert Hutkins May 2024

Identification Of Carbohydrate Gene Clusters Obtained From In Vitro Fermentations As Predictive Biomarkers Of Prebiotic Responses, Car Reen Kok, Devin J. Rose, Juan Cui, Lisa Whisenhunt, Robert Hutkins

Department of Food Science and Technology: Faculty Publications

Background Prebiotic fibers are non-digestible substrates that modulate the gut microbiome by promoting expansion of microbes having the genetic and physiological potential to utilize those molecules. Although several prebiotic substrates have been consistently shown to provide health benefits in human clinical trials, responder and non-responder phenotypes are often reported. These observations had led to interest in identifying, a priori, prebiotic responders and non-responders as a basis for personalized nutrition. In this study, we conducted in vitro fecal enrichments and applied shotgun metagenomics and machine learning tools to identify microbial gene signatures from adult subjects that could be used to predict …


Beyond The Human: Crossovers For An Onto-Epistemological Bifurcation, Ester Toribio-Roura May 2024

Beyond The Human: Crossovers For An Onto-Epistemological Bifurcation, Ester Toribio-Roura

Articles

Building upon recent studies in new materialisms and feminist critical posthumanism with a focus on human and more-than-human relationships, this paper examines how the posthuman paradigm, by postulating the queering of identit(ies) via entanglement with the more-than-human (including technology), and by offering a critical examination of diverse modes of existence within a broader ecological context, can foster more inclusive and ethically sound ways of being in the world. Although posthumanism encompasses a wide range of perspectives and theories, including transhumanism, at its core, it challenges traditional notions of humanism, blurring the boundaries between what is human and what is more-than-human, …


Factors Affecting Adherence To Antiretroviral Therapy Among People Living With Hiv/Aids In Antigua And Barbuda, Yuliecia Antoinette Rowe-Harris Mrs, Chirlynor Ebora Calbayan Mrs, Kepha Odiwuor Pondi Mr May 2024

Factors Affecting Adherence To Antiretroviral Therapy Among People Living With Hiv/Aids In Antigua And Barbuda, Yuliecia Antoinette Rowe-Harris Mrs, Chirlynor Ebora Calbayan Mrs, Kepha Odiwuor Pondi Mr

Adventist Human-Subject Researchers Association

This study examined factors affecting adherence to antiretroviral therapy (ART) among people living with HIV/AIDS in Antigua and Barbuda. The findings identified multiple variables, emphasizing the dynamic components affecting ART adherence. These findings address a local gap and provide insights into the unique adherence challenges faced by a vulnerable population.


Effects Of Mechanical Processing On The Physicochemical Properties Of Oat Β-Glucan And Its In Vitro Fermentation, Li Hang, Wang Jing, Cheng Anwei, Wang Xufeng, Luo Kaiyun May 2024

Effects Of Mechanical Processing On The Physicochemical Properties Of Oat Β-Glucan And Its In Vitro Fermentation, Li Hang, Wang Jing, Cheng Anwei, Wang Xufeng, Luo Kaiyun

Food and Machinery

Objective: To evaluate the effects of mechanical processing on the physicochemical properties of oat β-glucan and its in vitro fermentation. Methods: Oat coarse grains, oat flakes, and oat flour were respectively produced through mechanical progressing methods such as steel cutting, table pressing, and grinding. Then, scanning electron microscopy was used to observe the microstructure, the content, dissolution rate and relative molecular weight of oat β-glucan were determined, and the colon environment was simulated in vitro for fermentation. Results: The total β-glucan content and dissolution rate of steel-cutting oat were higher than those of untreated sample. The total …


Determination Of Six Human Milk Oligosaccharides In Dairy Product By Precolumn Derivatization High Performance Liquid Chromatography With Fluorescence Detection, Li Qiqi, Huang Jiale, Mu Shuyi, Li Qishan, Huang Jingjun May 2024

Determination Of Six Human Milk Oligosaccharides In Dairy Product By Precolumn Derivatization High Performance Liquid Chromatography With Fluorescence Detection, Li Qiqi, Huang Jiale, Mu Shuyi, Li Qishan, Huang Jingjun

Food and Machinery

Objective: To astablish a strong popularity, high sensitivity of six human milk oligosaccharides in dairy product by precolumn derivatization high performance liquid chromatography-fluorescence method. Methods: Aquatic product sample was precipitated with glacial acetic acid, filtration by using the filter paper. It was derived by 2-aminobenzamide solution. The extracts were centrifuged and filtered by using the organic filter membrane and analyzed by using a AdvanceBio Glycan Map column to be separated and detected,employing acetonitrile-50 mmol/L ammonium formate solution (pH 4.4) as the mobile phase for gradient elution. Results: Under the experimental conditions, six human milk oligosaccharides in dairy product were linear …


The Establishment Of Arrhenius Prediction Model For Tomato Quality Under Extreme Meteorological Temperatures, Ma Minzhen, Wang Ji, Shi Ce, Jia Zhixin, Xu Qian May 2024

The Establishment Of Arrhenius Prediction Model For Tomato Quality Under Extreme Meteorological Temperatures, Ma Minzhen, Wang Ji, Shi Ce, Jia Zhixin, Xu Qian

Food and Machinery

Objective: A coupled model for predicting tomato quality during short-term storage and transportation under extreme temperatures, utilizing the Arrhenius equation in conjunction with meteorological temperature data, to forecast the quality changes in tomatoes during the short-term storage and transportation processes under extreme weather conditions. Methods: Analyzing the meteorological temperature data for the year 2020 in Weifang, Baoding, and Daxing, 7 temperature points were selected to simulate the range of temperature variations for short-term storage and transportation of tomatoes. By utilizing the Arrhenius equation and integrating temperature with weight loss rate, hardness, color difference (ΔE), and sensory evaluation scores …


Research Progress On Postharvest Quality Deterioration And Preservation Technology Of Blueberry, She Jiehai, Zhou Wenhua, Ma Yan, Zeng Yaoying, Yu Zhuojun, Guo Hangyu May 2024

Research Progress On Postharvest Quality Deterioration And Preservation Technology Of Blueberry, She Jiehai, Zhou Wenhua, Ma Yan, Zeng Yaoying, Yu Zhuojun, Guo Hangyu

Food and Machinery

Because of high moisture content, blueberries are susceptible to disease and insect infestation leading to corruption and deterioration, which has serious influence on the shelf life of blueberry fruits. This article summarizes the cause mechanism of postharvest quality deterioration of blueberry fruits and the research progress of blueberry preservation technology at home and abroad, mainly including physical, chemical and biological preservation methods; and emphasizes the application of biological methods in the postharvest preservation of blueberries.


Comparison Of The Activities And Cryoprotective Mechanism Of Two Antifreeze Peptides Derived From Silver Carp Parvalbumin, Li Jun, Fu Yuting, Yu Jian, Li Xianghong, Liu Yongle, Wang Faxiang May 2024

Comparison Of The Activities And Cryoprotective Mechanism Of Two Antifreeze Peptides Derived From Silver Carp Parvalbumin, Li Jun, Fu Yuting, Yu Jian, Li Xianghong, Liu Yongle, Wang Faxiang

Food and Machinery

Objective: Explore the cryoprotective mechanism and structure-activity relationship of antifreeze peptides derived from silver carp parvalbumin. Methods: Using differential scanning calorimetry and molecular dynamics simulation to compare activity, structure and mode of action of two typical peptides. Results: Pv-AFP 1 (KAADSFNH KAFFAKVG) had a stable α-helix structure, while Pv-AFP 2 (KAADSFNHKAF) tended to exhibit random coil. The thermal hysteresis activity of Pv-AFP 1 was 0.87 ℃, which was higher than Pv-AFP 2 (0.74 ℃). Molecular dynamics simulations showed that Pv-AFP 1 could interact with 53 water molecules, and could form 16 hydrogen bonds to adsorb onto the surface of …


Enrichment Of Γ-Aminobutyric Acid Based On Rice Heating And Humidification Technology, Xu Bin, Mi Xingqiong, Bai Qiong, Xu Jinlong, Liu Shuyi May 2024

Enrichment Of Γ-Aminobutyric Acid Based On Rice Heating And Humidification Technology, Xu Bin, Mi Xingqiong, Bai Qiong, Xu Jinlong, Liu Shuyi

Food and Machinery

Objective: This study aimed to improve the quality of brown rice. Methods: The content of γ-aminobutyric acid (GABA) and the activity of glutamate decarboxylase were used as indicators to study the effect of enrichment conditions on GABA content. Based on the optimal parameters, GABA content in each part of the grain was analyzed to reveal the migration law of GABA. Finally, the effect of the method on the quality of brown rice was analyzed and compared with the soaking method. Results: The highest GABA content was obtained when the water content of the raw material was 14.5% with the final …


Effects Of Different Ultrasonic Enhancement Methods On The Quality Characteristics Of Dried Lotus Root In Heat Pumps, Li Xuhao, Zhong Jinghan, Li Fang, Liu Yunhong May 2024

Effects Of Different Ultrasonic Enhancement Methods On The Quality Characteristics Of Dried Lotus Root In Heat Pumps, Li Xuhao, Zhong Jinghan, Li Fang, Liu Yunhong

Food and Machinery

Objective: This study aimed to explore the effect of different ultrasonic enhancement methods on the quality characteristics of dried lotus root in heat pumps. Methods: The effects of US, CU, and USOD on the drying characteristics, appearance color, microstructure, texture characteristics, nutritional quality and antioxidant characteristics of the lotus root heat pump during the drying process were investigated. Ultrasonic (US), direct contact ultrasound (CU), USOD and heat pump drying (HPD) were treated in series and parallel. The coefficient of variation method was used to comprehensively evaluate the quality of lotus root dried by different ultrasonic enhancement methods. Results: US, CU …


Preparation, Structural Characterization, And Performance Analysis Of Polyvinyl Alcohol/Fish Gelatin/Solanum Nigrum Anthocyanin Composite Film, Ouyang Lanlan, Deng Na, Yin Shixian, Rong Zhixing, Wang Jianhui May 2024

Preparation, Structural Characterization, And Performance Analysis Of Polyvinyl Alcohol/Fish Gelatin/Solanum Nigrum Anthocyanin Composite Film, Ouyang Lanlan, Deng Na, Yin Shixian, Rong Zhixing, Wang Jianhui

Food and Machinery

Objective: Screening of polyvinyl alcohol-fish gelatin-solanum nigrum anthocyanin (PVA/FG-SNA) composite packaging film with optimal structure and properties. Methods: Optimizing the fabrication process of films through orthogonal tests, and using thickness, moisture content, water vapor permeability (WVP), tensile strength (TS), elongation at break (EB), Fourier infrared spectroscopy (FTIR), X-diffraction (XRD), scanning electron microscopy (SEM), and thermogravimetric (TG) analysis to explore the effects of SNAs with different mass fractions on the properties and microstructures of PVA-FG film; In addition, the pH color response and NH3 sensitivity of the PVA/FG-SNA composite film were monitored. Results: When the mass ratio of PVA-to-FG, heating …


Study On Hypoglycemic And Lipid-Lowering Activity Of Shrimp Shell-Derived Enzymatic Hydrolysate And Peptide Sequence Function Analysis, Han Pengwei, Yi Tong, Li Honghui, Wu Hao, Li Yan, Chu Wuying May 2024

Study On Hypoglycemic And Lipid-Lowering Activity Of Shrimp Shell-Derived Enzymatic Hydrolysate And Peptide Sequence Function Analysis, Han Pengwei, Yi Tong, Li Honghui, Wu Hao, Li Yan, Chu Wuying

Food and Machinery

Objective: The PCSPHs were prepared by enzymatic hydrolysis of Procambarus clarkii shells, and their hypoglycemic and lipid-lowering activities in vitro were evaluated and peptide sequence were analyzed. Methods: Different crayfish shell proteolysates were prepared by hydrolysis of pepsin, alcalase protease, trypsin, flavor protease and papain, and their in vitro hypoglycemic and lipid-lowering activities were evaluated and peptide sequences were determined. The peptides sequence of Procambarus clarkii shells was identified by LC-MS/MS. Taking the crystal structure of the nuclear receptor PPARγ ligand binding region as the target, Autodock vina was used to simulate molecular docking to obtain crayfish shell peptides …


Nutritional Evaluation And Enzymatic Hydrolysis Peptide Preparation Process Optimization Of Aspongopus, Li Jiaxu, Yang Yang, Wang Biying, Li Pan, Du Bing May 2024

Nutritional Evaluation And Enzymatic Hydrolysis Peptide Preparation Process Optimization Of Aspongopus, Li Jiaxu, Yang Yang, Wang Biying, Li Pan, Du Bing

Food and Machinery

Objective: This study aimed to investigate the nutrient composition and the optimal enzymatic conditions for the peptides of Aspongopus and lay the groundwork for the creation of Aspongopus protein resources. Methods: The fat, protein, crude polysaccharide content, and fatty acid composition were determined according to the national standard method, and the total flavonoid and polyphenol content was determined by the standard curve method. Response surface methodology was used to optimize the enzymatic process conditions of Aspongopus based on the peptide yield; Amino acid analyzer and high-performance gel permeation chromatography was used to determine the amino acid composition and the relative …


Study On The Numbing Substance Of Polygonatum Rhizoma Based On Steaming Treatment, Wang Jiaxuan, Wang Feng, Li Qingming, Li Wenjia, Su Xiaojun May 2024

Study On The Numbing Substance Of Polygonatum Rhizoma Based On Steaming Treatment, Wang Jiaxuan, Wang Feng, Li Qingming, Li Wenjia, Su Xiaojun

Food and Machinery

Objective: To discover the material substances of the numbing characteristics in Polygonatum Rhizoma. Methods: Polygonati Rhizoma from eight regions were processed by the "nine-steam-nine-bask" method, and its content changes of saponin, polyphenol and calcium oxalate crystal at different steaming times were determined. The degree change of numbing was determined by the cell hemolysis test and sensory properties, and a correlation analysis of numbing was conducted. Results: With the increase of steaming times, the contents of polyphenol and saponin in Polygonati Rhizoma from different regions were initially increased and then decreased, while the content of calcium oxalate crystal and hemolysis …


Hot Air Drying Characteristics And Model Of Mango Slices, Huang Min, Gan Ting, Yi Ping, Huang Fang, Li Li May 2024

Hot Air Drying Characteristics And Model Of Mango Slices, Huang Min, Gan Ting, Yi Ping, Huang Fang, Li Li

Food and Machinery

Objective: In order to predict and control the hot air drying process of Mango. Methods: Using fresh Mangifera indica Linn as experimental material, the effects were studied in different hot air temperatures (60, 65, 70 ℃) and different thickness of Mango slices (0.8, 1.0, 1.2 cm) on the drying curve, drying characteristic curve and effective moisture diffusion coefficient of Mango. Selecting six commonly used drying models suitable for fruits and vegetables for fitting, analysis, and validation, the most suitable model for Mango hot air drying was selected. Results: With the increase of temperature and the decrease of slice thickness, the …


Optimization Of Preparation Process And Preliminary Structure Characterization Of Polysaccharides From Lemon Peel With High Hypoglycemic Activity, Jiang Zhonggui, Li Di, Li Kaifeng, Ma Li, Gu Xin May 2024

Optimization Of Preparation Process And Preliminary Structure Characterization Of Polysaccharides From Lemon Peel With High Hypoglycemic Activity, Jiang Zhonggui, Li Di, Li Kaifeng, Ma Li, Gu Xin

Food and Machinery

Objective: This study aimed to optimize the preparation process of polysaccharides from lemon peel and screen out the components with high hypoglycemic activity. Methods: Ultrasonic assisted enzymatic extraction of lemon peel polysaccharide, combined with response surface test to explore the best extraction process, through DEAE-52 anion chromatography and Sephadex G-100 gel column chromatography separation and purification to obtain a homogeneous polysaccharide fraction (named as LPs-1a). The effective hypoglycemic components of lemon peel polysaccharides were screened by α-glucosidase and their relative molecular weight, monosaccharide composition and characteristic groups were analyzed. Results: The optimal extraction process of lemon peel polysaccharide was …


Research On Efficient Production And Quality Optimization Of Yuba Based On Soymilk Solids Concentration And Slurry Depth Control, Zhang Yan, Ren Guangyue, Duan Xu, Liu Wenchao, Wang Zhe May 2024

Research On Efficient Production And Quality Optimization Of Yuba Based On Soymilk Solids Concentration And Slurry Depth Control, Zhang Yan, Ren Guangyue, Duan Xu, Liu Wenchao, Wang Zhe

Food and Machinery

Objective: Determine the appropriate soymilk concentration and slurry depth to provide a theoretical reference for the efficient and high-quality production of yuba. Methods: The effect of soymilk solids concentration and slurry depth on the yield, film-forming rate, nutrient contents, mechanical properties, cooking loss rate, and rehydration ratio were investigated. Results: The optimal yield solids of yuba (34.68%) and highest nutritional value occurs when the soymilk solids concentration is 6% and the soymilk slurry depth is 6 cm, which presents 51.05% protein, 23.42% fats, and it also has good tensile strength and rehydration qualities, with respective measurements of 3.74 MPa and …


Study On Optimization Of Enzymatic Hydrolysis Technology For Mushroom Sauce, Chen Jing, Tang Haoguo, Wang Jiakang, Si Qihe, Shen Ruxiao May 2024

Study On Optimization Of Enzymatic Hydrolysis Technology For Mushroom Sauce, Chen Jing, Tang Haoguo, Wang Jiakang, Si Qihe, Shen Ruxiao

Food and Machinery

Objective: This study aimed to prepare a kind of mushroom paste with Lentinus edodes, Pleurotus eryngii and Agaricus bisporus as raw materials by enzymolysis with cellulase. Methods: The response surface method was used to optimize the optimal enzymatic hydrolysis process of the mushroom paste with amino acid nitrogen content as the index. Results: The optimal enzymolysis conditions were as follows: enzyme dosage 0.6%, pH 6, enzymolysis temperature 50 ℃, solid-liquid ratio 1∶30 (g/mL). Under these conditions, the actual amino acid nitrogen content was 0.418 μg/100 g. Conclusion: The sensory evaluation and nutritional indexes of the mushroom paste are up …


Preparation Of Matrix Reference Materials Of Bifenthrin And Cyhalothrin Pesticide Residues In Green Tea, Fang Haixian, Chen Xinglian, Li Xinyun, Li Jun, Pu Yali, Liu Hongcheng May 2024

Preparation Of Matrix Reference Materials Of Bifenthrin And Cyhalothrin Pesticide Residues In Green Tea, Fang Haixian, Chen Xinglian, Li Xinyun, Li Jun, Pu Yali, Liu Hongcheng

Food and Machinery

Objective: The preparation method of matrix reference materials of bifenthrin and cyhalothrin pesticide residues in green tea was established. Methods: Green tea samples were sprayed with biphenylthrin and cyhalothrin. The samples were crushed, screened, homogenized and bottled. After the homogeneity and stability tests, the quantity value was determined by three laboratories, and the uncertainty was evaluated. Results: The amount values of bifenthrin and cyhalothrin in green tea were (0.185±0.019 7) and (0.207±0.046 6) mg/kg with k=2, and the confidence interval was 95%. Conclusion: The developed matrix reference materials of bifenthrin and cyhalothrin in green tea are homogeneous and stable …


Characteristics Influencing Enrollment Choices: A Mixed Methods Analysis Of Factors That Influence Black Students' Decision To Enroll In College Of Agriculture Programs, Jennifer L. Brown May 2024

Characteristics Influencing Enrollment Choices: A Mixed Methods Analysis Of Factors That Influence Black Students' Decision To Enroll In College Of Agriculture Programs, Jennifer L. Brown

LSU Doctoral Dissertations

This study aims to identify the barriers that affect Minority enrollment in agriculture, particularly focusing on increasing the enrollment of Black students. There is a significant concern regarding the gap between Black individuals and agriculture enrollment in post-secondary agriculture programs at land-grant institutions. The purpose of this mixed method phenomenology was to (1) identify factors that influence the decision of Black students to enroll in the College of Agriculture and (2) uncover the societal and economic influences that continue to serve as barriers to Black students’ enrolling in agriculture. The quantitative portion of the investigation utilized a web-based instrument structured …


Selective Refueling Of Car T Cells Using Ada1 And Cd26 Boosts Antitumor Immunity, Yue Hu, Abhijit Sarkar, Kevin Song, Sara Michael, Magnus Hook, Ruoning Wang, Andras Heczey, Xiaotong Song May 2024

Selective Refueling Of Car T Cells Using Ada1 And Cd26 Boosts Antitumor Immunity, Yue Hu, Abhijit Sarkar, Kevin Song, Sara Michael, Magnus Hook, Ruoning Wang, Andras Heczey, Xiaotong Song

Faculty, Staff and Student Publications

Chimeric antigen receptor (CAR) T cell therapy is hindered in solid tumor treatment due to the immunosuppressive tumor microenvironment and suboptimal T cell persistence. Current strategies do not address nutrient competition in the microenvironment. Hence, we present a metabolic refueling approach using inosine as an alternative fuel. CAR T cells were engineered to express membrane-bound CD26 and cytoplasmic adenosine deaminase 1 (ADA1), converting adenosine to inosine. Autocrine secretion of ADA1 upon CD3/CD26 stimulation activates CAR T cells, improving migration and resistance to transforming growth factor β1 suppression. Fusion of ADA1 with anti-CD3 scFv further boosts inosine production and minimizes tumor …


Impact Of The Department Of Defense Maternity Leave Policy Change On Retention And Postpartum Depression And The Air Force Postpartum Physical Fitness Policy Change On Physical Readiness Outcomes, Minette S. R. Herrick May 2024

Impact Of The Department Of Defense Maternity Leave Policy Change On Retention And Postpartum Depression And The Air Force Postpartum Physical Fitness Policy Change On Physical Readiness Outcomes, Minette S. R. Herrick

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Understanding the implications of health policy is paramount to support continued execution and further wide-spread distribution. The Department of Defense has made many recent family friendly policy changes in effort to improve the health and morale of the force. Two such policies were analyzed in this study. The first was the change in maternity leave, in 2016, from allotting women six weeks time off to twelve weeks time off in the postpartum period. The second was executed by the Air Force to permit postpartum women a longer duration for their required physical fitness assessment (an increase from six months to …


The Influence Of Stage Of Ripeness On Consumer Perceptions Regarding Selected Aspects Of Non-Astringent Asian Persimmons (Diospyros Kaki Thunb), Robert A. Mirabello Jr May 2024

The Influence Of Stage Of Ripeness On Consumer Perceptions Regarding Selected Aspects Of Non-Astringent Asian Persimmons (Diospyros Kaki Thunb), Robert A. Mirabello Jr

LSU Doctoral Dissertations

Asian Persimmon (Diospyros kaki Thunb.) is an important fruit crop in Asia and in recent decades has experienced expanding production around the world. Non-astringent cultivars are highly desirable in world markets. In the United States the Asian Persimmon is lesser known and consumed although it can be successfully grown in California and the Southeastern United States.This novel study examined the physical/chemical properties of the non-astringent Asian Persimmon ‘Jiro’ grown in Southern Louisiana at three distinct stages of ripeness described as Green (mid-September harvest), Light Orange (mid-October harvest) and Dark Orange (mid-November harvest). Data collected included color, weight, size, soluble …


Caffeine’S Impact On Heart Rate Variability During Short-Term Exercise In University Students, Maya J. Spinler, Morgan M. Payne, Christopher Carroll Dr. May 2024

Caffeine’S Impact On Heart Rate Variability During Short-Term Exercise In University Students, Maya J. Spinler, Morgan M. Payne, Christopher Carroll Dr.

Applied Health Sciences Student Works

The primary objective is to explore the physiological effects of caffeine on intra-bout exercise-induced blood pressure (BP) and heart rate (HR) deceleration resulting from repeated anaerobic exercise cycling in active university students. Use of caffeinated stimulants has increased in recent years, particularly in the exercise setting. The cardiovascular system (CS) is an essential piece of exercise physiology and is controlled by the autonomic nervous system (ANS). Caffeine has a known effect on the CS during acute exercise. What is not well solidified is the connection between caffeine and exercise-induced markers, such as heart rate recovery (HRR) and BP. Participants underwent …


Synthetic Biology Enablement: From Academic Development To Industrial Transformation, Yan Xiong, Xueqing Ma, Daming Chen, Xiao Liu, Guoping Zhao May 2024

Synthetic Biology Enablement: From Academic Development To Industrial Transformation, Yan Xiong, Xueqing Ma, Daming Chen, Xiao Liu, Guoping Zhao

Bulletin of Chinese Academy of Sciences (Chinese Version)

Synthetic biology revolutionizes the comprehension of life systems from an engineering perspective, employing a “bottomup” approach in life science research. It adopts an iterative research paradigm of “design-build-test-learn” in life science research and creates engineered new life systems grounded in genomics and systems biology. This provides a new pathway of “from creation to understanding” for life sciences, departing from the traditional reductionist research strategy of “study the whole to understand the parts” and opening up a new culture of “building to understand” the essence of life. Additionally, synthetic biology elevates existing biotechnologies previously based on “simulating natural processes” and “genetic …


Condition Scoring Of Sheep, Department Of Primary Industries And Regional Development, Western Australia May 2024

Condition Scoring Of Sheep, Department Of Primary Industries And Regional Development, Western Australia

Animal production and livestock factsheets

Condition scoring sheep is an easy and accurate method of estimating the condition or 'nutritional wellbeing' of your sheep flock. It requires an assessment of the amount of muscle and fat covering the backbone and the short ribs of each sheep. This gives a picture of the sheep’s store of energy. It is the best method for monitoring pregnant and lactating ewes but is also useful for monitoring the growth of weaners.


Revealing Microbial Community Composition And Disinfection Efficacy Evaluation In Wholesale Pork Markets Through 16s Rdna Sequencing, Miao He, Lingyan Ma, Wentao Lv, Yingping Xiao, Hua Yang, Xiofan Wang, Jiangchao Zhao, Hangzhen Lan, Wen Wang, Xingning Xiao May 2024

Revealing Microbial Community Composition And Disinfection Efficacy Evaluation In Wholesale Pork Markets Through 16s Rdna Sequencing, Miao He, Lingyan Ma, Wentao Lv, Yingping Xiao, Hua Yang, Xiofan Wang, Jiangchao Zhao, Hangzhen Lan, Wen Wang, Xingning Xiao

Animal Science Faculty Publications and Presentations

This study has employed 16S rDNA analysis to elucidate the characteristics of the microbial community in the surroundings, facilities, and meat samples collected from a pork wholesale market with the aim of revealing bacterial contamination. In addition, the efficacy of sodium hypochlorite disinfectant was assessed by comparing the bacterial communities in the viable samples before and after disinfection. The bacterial abundances in meat, chopping boards, and knives were positively correlated (r > 0.95, p <  .05), suggesting potential cross-contamination risks during market transactions. The species richness indices for the external transport vehicles, pork hooks, and floors were significantly different from those of the samples taken after decontamination (p <  .05). Linear discriminant analysis Effect Size (LEfSe) analyses showed that Firmicutes, Proteobacteria, and Chloroflexi were significantly more abundant in the transaction period, whereas Bacteroidetes levels decreased compared to the clean-up period. NaClO disinfection …


Potential Enhancement Of Microbial Disinfection Using Oxygen Enriched Cold Atmospheric-Pressure Argon (Ar/O2) Plasma Jet, Waleed O. Younis, Mahmoud M. Berekaa, Mostafa A. Ellbban, Abdel-Sattar S. Gadallah, Jamal Q. Almarashi, Abdel-Aleam H. Mohamed May 2024

Potential Enhancement Of Microbial Disinfection Using Oxygen Enriched Cold Atmospheric-Pressure Argon (Ar/O2) Plasma Jet, Waleed O. Younis, Mahmoud M. Berekaa, Mostafa A. Ellbban, Abdel-Sattar S. Gadallah, Jamal Q. Almarashi, Abdel-Aleam H. Mohamed

Karbala International Journal of Modern Science

Oxygen activated cold-atmospheric-pressure-argon plasma jet (APPJ) has gained prominence over the regular argon plasma especially in disinfection and decontamination. As an objective of the current research, an oxygen-enriched argon system was built, where plasma produced through a vessel metallic tube that is introduced into alumina one. A sinusoidal high voltage signal of 25 kHz was used to generate plasma jet. Potential impact of oxygen enriched APP jet (Ar/O2) in decontamination of different microbial cells was observed. For examination, suspension of each tested microbe was placed in contact with plasma jet nearly 10 mm away from the jet nozzle …


Effects Of Fast And Slow-Wilting Soybean Genotypes On Fall Armyworm (Spodoptera Frugiperda) Growth And Development, Jessica Ayala, Alejandro Vasquez, Devi Balakrishnan, Evelyn Madrigal, Justin George, Rupesh Kariyat May 2024

Effects Of Fast And Slow-Wilting Soybean Genotypes On Fall Armyworm (Spodoptera Frugiperda) Growth And Development, Jessica Ayala, Alejandro Vasquez, Devi Balakrishnan, Evelyn Madrigal, Justin George, Rupesh Kariyat

Entomology and Plant Pathology Faculty Publications and Presentations

Soybean (Glycine max) is the most important plant protein source, and Fall Armyworm (FAW, Spodoptera frugiperda) is considered a major pest. This study aimed to examine the impact of FAW feeding on soybean accessions that vary in their water use efficiency (WUE) traits, by examining FAW growth and life history parameters along with plant growth response to pest damage. Soybean accessions were grown in a greenhouse and exposed to FAW larval feeding for 48 h at three different soybean growth stages: V3, R3, and R6. The growth and development of the FAW and soybeans were monitored. Results showed that faster …


Effects Of Fast And Slow-Wilting Soybean Genotypes On Fall Armyworm (Spodoptera Frugiperda) Growth And Development, Jessica Ayala, Alejandro Vasquez, Devi Balakrishnan, Evelyn Madrigal, Justin George, Rupesh R. Kariyat May 2024

Effects Of Fast And Slow-Wilting Soybean Genotypes On Fall Armyworm (Spodoptera Frugiperda) Growth And Development, Jessica Ayala, Alejandro Vasquez, Devi Balakrishnan, Evelyn Madrigal, Justin George, Rupesh R. Kariyat

School of Integrative Biological & Chemical Sciences Faculty Publications

Soybean (Glycine max) is the most important plant protein source, and Fall Armyworm (FAW, Spodoptera frugiperda) is considered a major pest. This study aimed to examine the impact of FAW feeding on soybean accessions that vary in their water use efficiency (WUE) traits, by examining FAW growth and life history parameters along with plant growth response to pest damage. Soybean accessions were grown in a greenhouse and exposed to FAW larval feeding for 48 h at three different soybean growth stages: V3, R3, and R6. The growth and development of the FAW and soybeans were monitored. Results showed …