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Relationships Between Radiophosphorus Accumulation By Bacteria, Algae, And Tubificid Worms In Laboratory Food Chains, Warren L. Stromberg

Masters Theses

No abstract provided.

A Study Of Water-Soluble Inhibitory Compounds (Algicides) Produced By Fresh-Water Algae, Denny O. Harris, Manhar C. Parekh

KWRRI Research Reports

A complex system of growth inhibitors was observed in the green algae (Volvocaceae). Inhibitors were found in the culture filtrates of some genera which limit their own growth (autoinhibitors) while others in the family produce substances which check the growth of other genera (heteroinhibitors). These inhibitors were destroyed by autoclaving. It was decided that Pandorina morum, which produced the strongest inhibitor and Volvox tertius, the most sensitive to the inhibitor would make an excellent model system for a study of the chemical and physical properties of these naturally occurring algicides. The algicide could be removed from actively growing cultures about …

The Effects Of Atropine On The Growth And Respiration Of Two Species Of Bacteria Normally Inhabiting The Human Gastro-Intestinal System, Mary M. Ramos

Theses & Dissertations

It is the purpose of this study to determine whether the drug atropine has an effect on bacteria found normally inhabiting the human body, most specifically the gastrointestinal system. Atropine is one of the oldest drugs in medicine. It is derived from the roots and leaves of solanaceous plants such as henbane and Jimson weed. Atropine is often termed a belladonna alkaloid because it can also be found in the deadly nightshade plant, Atropa belladonna. Atropine is a highly competitive antagonist of acetylcholine at receptor sites in smooth muscles, cardiac muscle, and various glandular cells (5). Therefore, its effects are …

Potential Insect-Control Applications For Microwaves, Stuart Nelson

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The idea of using radiofrequency (RF) electric energy for controlling insects is not new. Experiments in the United States to control insects with high-frequency electric fields date back at least 45 years, and reports indicate development of similar interests in Europe during the same period. Many researchers have explored the possibilities since then, and their findings have been reviewed in several publications (Thomas, 1952; Frings, 1952; Peredel'skii, 1956; van den Bruel et al., 1960; Watters, 1962; Nelson, 1966, 1967, 1972, 1973). The purposes in preparing this paper are to summarize these findings in a general way, to discuss some of …

Localization Of Deep Abscesses With Radioactive Isotopes: An Experimental Study, H. M. Allen, R. Berguer, L. A. Dusault, M. A. Block

Henry Ford Hospital Medical Journal

Life-endangering effects of deep abscesses make early and precise identification and localization important to permit adequate surgical drainage. An experiment was undertaken to study the feasibility of detecting abscesses by using leucocytes tagged with radioactive isotopes. For the labeling, 51chromium appeared preferable to 131iodine or 99technetium. Leucocytes so labelled will concentrate sufficiently in abscesses to permit identification of abscesses by scintillation scanning. For clinical application, techniques require further simplification and refinement to eliminate interference by collection of radioactive isotopes in the liver and spleen.

Nematosporaceae (Hemiascomycetidae): Taxonomy, Pathogenicity, Distribution, And Vector Relations, Lekh R. Batra

Ba

For almost a century (42, 139) certain yeast, or yeastlike parasites of fruits and seeds have been known. They occur on a wide variety of crop plants and cause such diseases as stigmatomycosis," "yeast-spot," "eye spot," and "internal rot." They seemingly represent a phylogenetic unit. They have cylindrical or pyriform asci, and the hyaline ascospores often are characteristically arranged in two fascicles lying end to end. All have acicular ascospores. (See figs. 2, a-c; 5 , c; 11, hand i; 12, A; 13, c-h; 15,j-k.) They have similar habitats, and several species may simultaneously occur in the same fruit or …

Normal And Pathogenic Bacteria Isolated From Conditioned, Non-Conditioned And Wild Baboons (Papio Cynocephalus), Louis H. Boncyk

Theses & Dissertations

This study is the summation of the aerobic findings of a five-year longitudinal study designed to catalogue and define the "normal" flora of a conditioned baboon. Also, for comparison and evaluation, the findings of baboons captured and sampled in the "wild" in 1968 or shipped to the SFRE facilities during 1965 or from August of 1968 to December of 1971 are included.

Machine Variables In Basis Weight Control, Barry L. Hess

Paper Engineering Senior Theses

The purpose of this study was to determine the significance of machine variables as they affect the scan average basis weight readings obtained from the Industrial Nucleonics basis weight gauge on the fourdrinier paper machine of the Department of Paper Science and Engineering at Western Michigan University. The variables used in this study were machine speed, machine chest and tray white water consistencies, tray white water flow, flow to the headbox, stock valve position and head on the stock valve. Each of the above variables was sampled every ten minutes.

There were definite correlations between basis weight variation and machine …

A Floral Survey Of The Lake Michigan College- Ross Property, Van Buren County, Michigan, William Monroe Knott

Masters Theses

No abstract provided.

The Effect Of Haloxon And Thiabendazole On The Free-Living Stages Of Haemonchus Contortus, Gary Mccallister

Theses and Dissertations

This study was undertaken to determine the effect of thiabendazole and haloxon on the free-living stages of Haemonchus contortus and to determine the suitability of the free-living stages for their use in primary drug evaluation for chemotherapy or for chemical control of free-living stages. Cleaned unembryonated ova, embryonated ova, first-, second-, third-, and exsheathed third-stage larvae were exposed to suspensions of 5.0%, 1.0%, 0.5%, 0.25%, 0.12%, 0.05%, and water soluble concentrations of each drug for exposure times of 0.25, 0.50, 1.0, 2.0, 4.0, 8.0, and 16.0 hours at pH 7.0 and 25° C. Survival was assessed by observing any subsequent …

Light And Electron Microscopy Investigation Of Developing Wheat Caryopses Infected By Tilletia, Melvin Douglas Grove

Theses and Dissertations

Caryopses of wheat were studied during infection by Tilletia caries and T. controversa. Initially the pathogens penetrated embryos of the host. After the embryo tissue was consumed by the fungi, endosperm tissue was penetrated and consumed. Initial penetration of endosperm tissue was in localized areas. During infection the fungus mycelium spread intercellularly, but occasionally was seen intracellularly. As the host tissue was consumed teliospores were formed. At maturity a host cell layer 4-6 cells thick remained which retained the mature teliospores. Histochemical studies indicated that carbohydrates and lipids of the host disappeared soon after invasion by the pathogens. Nuclei and …

Ultrastructure Of Histochemically Localized Cholinesterases In Central And Peripheral Nervous Systems Of Two Insects: Periplaneta Americana L. And Acheta Domestica L, Clifford James Stratton

Theses and Dissertations

Cholinesterase (ChE) was histochemically localized by three methods in the sixth abdominal ganglion and the femur of crickets and cockroaches using light and electron microscopy. The light microscopy showed that ChE in insect femur could be readily demonstrated. The ultrastructural data showed enzyme activity: in ganglion - neural lamella, perineurium, axolemma, inside axons, along glia and at probable synapses; in peripheral nerves - in glial cells, sarcoplasmic reticulum, T-system, axolemma, sarcolemma and at neuromuscular junctions. All activity was inhibited with 10-4 eserine except in the neural lamella, suggesting cholinesterase at all of the above sites except the neural lamella where …

The Effects Of Deficiencies Of Certain Mineral Nutrients On The Activity And Various Properties Of Ribulose-1,5-Diphosphate Carboxylase From Barley (Hordeum Vulgare L.), Philip Alan Harrison

Theses and Dissertations

Barley plants grown in Mg, Mn, Zn and Cu deficient media were analyzed for specific activity, total activity and amount of RuDPCase present. Percent dry weight, as well as chlorophyll and soluble protein content were determined. All deficiencies caused lower chlorophyll and RuDPCase concentrations than occurred in the control. Soluble protein content, however, was higher in all deficient plants than in the control, either on a dry or a fresh weight basis. The addition of 18 μM Mn^2+ increased the activity of Mg^2+ containing RuDPCase preparations as much as 133% above normal. The specific activity of RuDPCase from Mg, Mn, …

Physiological Comparison Of Plaque-Producing And Non-Plaque-Producing Streptococci With Emphasis On Mannitol Utilization, Brenda Castle

Morehead State Theses and Dissertations

A thesis presented to the faculty of the School of Sciences and Mathematics at Morehead State University in partial fulfillment of the requirements for the Degree of Master of Science in Biology by Brenda Castle on July 19, 1973.

Growth Studies On The Shigellae, Margaret Jane Gillespie

Biology ETDs

The growth of 20 strains of Shigella, representing the four species, was evaluated by the inoculation of pure cultures of single strains on Tryptic Soy agar (TSA) and four selective plating media. Colony counts of 18 strains were highest on TSA. While recovery on MacConkey (MAC) and Xylose Lysine Desoxycholate (XLD) agars was intermediate, very low counts occurred on Desoxycholate Citrate (DCA) and Salmonella-Shigella (SS) agars. Six strains plated on MAC and four strains plated on XLD gave colony counts 10% lower than TSA. With DCA and SS 15 and 16 strains, respectively, gave colony counts 50% lower than TSA. …

Experimentation To Develop Procedures To Be Used In The Investigation Of The Effects Of Restriction On The Unmodified Dna Of The Bacteriophage Lamda, John Haynes

Honors Theses

The results of experimentation approximately twenty years ago with variuos bacteriophages by Ralston and Krueger¹⁶, Anderson and Felix¹, Luria and Human¹², and by Bertani and Weigle² have shown that the host range of a given phage depends directly on the bacterial strain on which the phage has last propagated. This event came to be known as host-induced modification or host-controlled variation. As this phage host range is controlled by the host environment and can be altered from one generation to another it has, according to Luria, made microbiology "the last stronghold of Lamarckism" …

Purification And Characterization Of Dihydroorotase From Clostridium Oroticum, A Zinc-Containing Metalloenzyme, William Edward Balch

Dissertations and Theses

Dihydroorotase (4,5-L-dihydro-orotate amidohydrolase, EC 3.5.2.3) which catalyzes the reversible cyclization of N-carbamyl-1-aspartate toL-dihydro-orotate has been purified from orotate-grown Clostridium oroticum by a combination of streptomycin sulfate fractionation, DEAE-Sephadex chromatography, and hydroxylapatite chromatograpy. The enzyme has been shown to be omogeneous when subjected to polyacrylamide gel electrophoresis. Thin-layer gel chromatography with Sephadex G-200 indicated the enzyme to have a molecular weight of 110,000 ± 10,000. Sodium dodecyl sulfate gel electrophoresis using two different buffer systems indicate the enzyme to be composed of two identical subunits with a molecular weight of 56,000 ± 5300. Dihydroorotase has been shown to be a zinc-containing …

Texas Forestry Paper No. 23, J. J. Stransky

Texas Forestry Papers, No. 1-29, 1970-1976

"Alternatives in southern wildlife-timber management"

Experience With Cleft Lip And Palate At The Henry Ford Hospital, W. Peter Mccabe

Henry Ford Hospital Medical Journal

A detailed analysis is presented of 321 patients with clefts of the lip and/or palate treated at Henry Ford Hospital. Comments are made on past and future trends in the management of these complex entities.

Role Of Membrane-Associated Components Of Mycoplasma Arthritidis On The Biosynthesis Of Humoral Antibodies And Arthritogenic Properties In Experimental Animals, Lois M. Bergquist

Loma Linda University Electronic Theses, Dissertations & Projects

The virulence of animal-passaged Mycoplasma arthritidis (158) was demonstrated to be enhanced in rats by growth in oleic acid-enriched broth. The virulence of a laboratory-maintained culture of the same strain was unaffected by similar growth conditions. No differences either in the immune response as measured by complement-fixing (CF) antibodies or in the histogenesis of the disease were detected when rats were injected with animal-passaged M. arthritidis grown either in the presence or in the absence of oleic acid. All tests for metabolism-inhibiting (MI) antibodies in the rat were negative.

A Mycoplasma-associated immunosuppression of the primary and of the secondary hemagglutinin …

Production Of Iodinated Compounds And The Effect On Strobilation In Aurelia Aurita, William J. Welch

Morehead State Theses and Dissertations

A thesis presented to the faculty of the School of Sciences and Mathematics at Morehead State University in partial fulfillment of the requirements for the Degree of Master of Science in Biology by William J. Welch on May 2, 1973.

Changes In The (Na+ K+) - Dependent Atpase Activity In Gills Of The Euryhaline Teleost, Fundulus Heteroclitus, Upon Adaptation To Freshwater And Salt Water As Determined By Specific Activities And 3h - Ouabain Binding Sites, Mary Elizabeth Gilman

Master's Theses

Previous attempts to measure the (Na⁺+K⁺) - dependent ATPase in gills of euryhaline teleosts have yielded controversial results. In this study both the (Na⁺+K⁺) - dependent ATPase activity and the number of JH - ouabain binding sites, representing the number of ATPase molecules, were measured in gill tissue preparations of the killifish, Fundulus heteroclitus. The (Na⁺+K⁺) dependent ATPase activity and ouabain binding sites were greater in gills of fish fully adapted to freshwater than in those adapted to salinities of 17 ppt or 24 ppt. On the other …

Effects Of Temperature And Ph On The Growth And Composition Of The Sporophyte And Gametophyte Generations Of Allomyces Arbusculus Butler (1911), Lawrence Wayne Lenz

Master's Theses

Pure cultures of the sporophyte and gametophyte generations of Allomyces arbusculus Butler (1911) were grown under varied conditions of temperature and pH. On solid media the gametophyte did not grow at 10 and 15 C; the sporophyte began slow growth after a long lag period at 15 C. There were no significant differences in growth between the sporophyte and gametophyte at 20 and 25 C. Increasing colony areas occurred for both generations at temperatures between 30 and 40 C, with the largest amount of growth for either generation at 35 C.

Colonies grown in broth media at various pH levels …

Bacteriophage Φ6: A Lipid-Containing Virus Of Pseudomonas Phaseolicola, Anne K. Vidaver, R. K. Koski, James L. Van Etten

Department of Plant Pathology: Faculty Publications

The purification and properties of a lipid-containing bacteriophage, φ 6, are described. The phage contains a lipid envelope which is probably essential for infection. Infectivity of φ 6 was lost in the presence of organic solvents, sodium deoxycholate, and phospholipase A. The fatty acid composition of the phage lipid was similar to that of the Pseudomonas phaseolicola host cells. The phage was composed of about 25% lipid, 13% RNA, and 62% protein. The buoyant density of φ6 was 1.27 g/ml in cesium chloride. The morphology of φ6 was unusual; it had a polyhedral head of about 60 nm surrounded by …

Studies On Some Bacteriolytic Bacteria Isolated From An Oxidation Pond, Paul Christian Radich

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

During bacteriological studies of the Logan City Sewage Oxidation Ponds, Logan, Utah, a group of bacteria was discovered which could lyse killed cells of Escherichia coli. The present research effort was an attempt to determine whether two isolates from this earlier study might play a role in the reduction of coliforms and other bacteria in the oxidation ponds. It was also desired to characterize partially the lytic enzymes produced by the two isolates and to determine as far as possible the organisms' identity.

Both isolates were Gram negative rod-shaped organisms with a single polar flagellum. On Cytophaga agar they …

Analysis Of Hunter Characteristics And Attitudes Relating To Utah Shooting Preserves, John T. Ratti

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This paper evaluates the present status of Utah shooting preserves with special reference to attitudes and characteristics of hunters using and not using the shooting preserves system. Data were gathered primarily by a mail questionnaire survey.

Compared to non-users, shooting preserve users were more frequently raised in a suburban or city area, better educated, and had higher yearly incomes. Shooting preserve users were commonly employed as professionals or proprietors, while non-users were often employed as craftsmen, proprietors, or operators.

Most shooting preserve hunters hunted after the state game bird season was closed, and were generally satisfied with Utah preserves. Most …

The Development Of An Improved Milk Substrate For Rennet Coagulation Assay On An Automatic Clot-Timer, Mark Brimhall Stevens

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

A substrate was developed for measuring the milk clotting strength of rennet preparations on an automatic clot-timer. The substrate contained 8 percent pasteurized skim milk solids, 1 percent chloroform, 0.3 percent 200 bloom gelatin, 0.03 M added CaCl₂ and was buffered to pH 6.6 with 0.057 M cacodylic acid and 0.042 M triethanolamine. The substrate was shelf-stable for 18 days at room temperatures. It was found that rennet preparations could be standardized to within 1 percent of each other, in terms of milk clotting strength, by use of the substrate on the automatic clot-timer. The method appears to have …

Phosphate Requirements For A Whey-Based Lactic Bacteriophage Resistant Medium Under Ph Control, Napasri Ausavanodom

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Five strains of lactic cultures and their homologous bacteriophage races were examined for response in a whey-based medium under pH control and fortified with zero to two percent levels of added orthophosphates. One percent each of culture and phage was inoculated into each medium, which was incubated at 25 C and continuously neutralized at pH 6.3 with 20 percent NH₄OH. Growth response in media was compared against growth in non fat dry milk and a commercial phage inhibitory medium (Marstar).

The whey-based medium which had been frozen and thawed provided more effective growth response and greater phage protection …

Regulation Of Rabbit Testicular Capsular Motility: Prostaglandins, Sex Steroids, And Sympathomimetic Agents, Rod R. Seeley

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Smooth-muscle active compounds were extracted from rat and rabbit testicular homogenates, and from bathing media of rabbit testicular capsular preparations and were tentatively characterized as prostaglandins. The endogenous prostaglandins were responsible for spontaneous testicular capsular contractions in vitro, but were not solely responsible for spontaneous testicular capsular motility in vivo. In this respect, significantly greater concentrations of prostaglandins were extracted from bathing media from spontaneously contracting capsules in vitro than from inactive capsules in vitro or spontaneously contracting capsules in vivo. No significant difference was observed in the amount of prostaglandin extracted from the bathing media of …

Control Of Rat Testicular Monoamine Oxidase Activity: Age, Seasonality, Melatonin, Hcg, Fsh, Starvation And Irradiation, Ronald Lee Urry

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

A number of investigations were undertaken to determine what factors control testicular MAO activity. Activity was high in neonatal rats (19 g - body weight), showed a reduced activity at 53 g, was increased at 145-235 g and showed a progressively reduced activity at 415 g, 482 g and 650 g. Hypophysectomy of immature male rats at 3 weeks of age, or mature rats at 10 weeks of age, significantly reduced testicular weight and total MAO activity as calculated on a per animal basis. The specific activity of the enzyme, as expressed on a per mg of tissue basis, was …