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Sclerosing Cholangitis: Surgical Significance, C. Grodsinsky, M. A. Block, B. E. Brush

Henry Ford Hospital Medical Journal

The authors' experience with the rare entity of sclerosing cholangitis suggests that several anatomical patterns of involvement of the extrahepatic biliary tract may occur. The involvement may include the intraluminal ducts segmentally or diffusely, or the process may primarily be external to the ducts as pericholedochitis. It may or may not represent one component of a systemic disease, particulady autoimmune disease. Although involvement may be diffuse, progression may be slow and compatible with many years of life. Long time followup is essential before any value can be assigned to a given modality of treatment. Carcinoma of the ductal system may …

Left Atrial Myxoma With An Atrial Septal Defect: Report Of A Case, Daniel T. Anbe, Edward Arciniegas, Henry Green, Ellet H. Drake

Henry Ford Hospital Medical Journal

A case of a left atrial myxoma presented unusual features including (1) association with an atrial septal defect, (2) attachment of the myxoma to the posterior wall of the left atrium, (3) probable paradoxical pulmonary embolization from the left atrium, and (4) probable ball valve effect of the myxoma in the atrial septal defect.

A Study Of Naturally Occurring Algicides Produced By Freshwater Algae, Denny O. Harris, Harry D. Caldwell

KWRRI Research Reports

The mode of action of the algicide produced by Pandorina morum was examined by exposing Volvox globator and isolates spinach chloroplasts to a partially purified algicide preparation. Oxygen evolution of Volvox, whole chloroplasts and broken chloroplasts (minus the Calvin cycle),was reduced indicating that the algicide inhibits the light reactions of photosynthesis. Oxygen evolution studies of other Volvocaceae confirmed the observation that Pandorina morum is not significantly influenced by its own inhibitor. Molecular weight approximation by gel filtration established that the inhibitor has a low molecular weight (probably below 100 mw). Field studies indicate that this algicide has tremendous potential as …

Louisiana Agriculture Magazine, Fall 1974, Lsu Agricultural Experiment Station

Louisiana Agriculture

No abstract provided.

Metabolic Capabilities Of Sulfur Oxidizing Bacteria And Their Role In Water Pollution, M. I. H. Aleem

KWRRI Research Reports

This report describes investigations into the physiology of microorganisms that are commonly involved in the oxidation of inorganic sulfur compounds. The metabolic activities of these bacteria play a potent role in several fields of economic importance such as strip mining operations, water pollution, corrosion, metallurgy, petroleum technology and soil fertility processes.

The oxidation of inorganic sulfur compounds was studied in the chemolithotrophs Thiobacillus denitrificans, Thiobacillus A₂, Thiobacillus neapolitanus, and a photolithotroph Rhodopseudomonas palustris. Cell suspensions from all of the thiobacilli catalyzed completely the oxidation of sulfide, thiosulfate, and sulfite to sulfate. The oxidation of thiosulfate in R. palustris …

A Study Of Rna Bacteriophage 7s Infection Of Pseudomonas Aeruginosa, Deanne Benson

Dissertations and Theses

A study was conducted to find the effect of magnesium, calcium, manganese and zinc ions on the infection of Psudomonas aeruginosa strain 1C by RNA bacteriophage 7s. When an 18 hour progeny experiment was performed, it was found that magnesium, calcium and manganese had different effects on bacteriophage production and was dependent on the bacterial growth conditions. RNA bacteriophage 7s progeny production was significantly enhanced by the addition of magnesium to cultures of Psudomonas aeruginosa 1C grown in a magnesium deficient medium. Under these environmental conditions there was a slight increase in progeny in the presence of calcium. When Psudomonas …

Enzyme Reactions Using Ureidosuccinate As A Substrate During Pyrimidine Biosynthesis And Degradation In Cl. Oroticum, Penny Amy

Dissertations and Theses

Cells of Clostridium oroticum, an anaerobic bacterium, were grown on orotate as a carbon and energy source. Ureidosuccinase, an inducible enzyme in the pathway for pyrimidine degradation has been shown to convert ureidosuccinate to aspartate, CO₂ and NH₃ as reported by Liebenmm and Kornberg (7). Aspartate and CO₂ were formed in approximately a 1:1 ratio from ureidosuccinase activity.

Ureidosuccinase was found to be a Mn+² requiring enzyme with a pH optimum of approximately pH 6.5. Enzyme activity is labile to O₂, temperature, pH, dilution and high ionic strength. The optimum conditions for storage …

Accumulative Coliform Population In A Five-Mile Section Of The Cibolo Creek, Norman Merrill Brown

Theses & Dissertations

A study of the accumulative coliform population in a five-mile section of Cibolo Creek near Schertz, Texas.

The greatest danger associated with water is that it may recently have been contaminated by sewage or by human excrement. Therefore, the protection of public water supplies from intestinal contamination is a necessary obligation of the public health authorities. If there happens to be a break in the protective chain (adequate treatment, disinfection, and protection of water supply), a chance exposure to fecal contamination could trigger an explosive outbreak of disease within a community. Modern technology and more sophisticated bacteriological methods have made …

Biochemical Parameters Of Hereditary Chondrodysplasia In Mice, Trent D. Stephens

Theses and Dissertations

Cartilage from newborn mice homozygous for chondrodysplasia contains normal quantities of protein and chondroitin sulfate. Mutant fetal cartilage incorporates normal amounts of sulfate, glucose, and glucosamine. Overnight rinsing with water removes about 25% of the incorporated sulfur and existing uronic acid from mutant ribs and about 55% from mutant limbs. Control ribs and limbs are affected very little by such a rinse. The diffusability of chondroitin sulfate (represented by incorporated sulfur and uronic acid) may be due to a lack of covalent bond formation between chondroitin sulfate and collagen.

Infection Of Smallmouth Bass And Goldfish By Two Species Of Saprolegnia, Cheryl A. Mcginley

Dissertations and Theses

This study consisted of several experiments designed to compare the ability of two species of fungi, Saprolegnia ferax and S. parasitica to grow on two species of fish, smallmouth bass (Micropterus dolomieui) and goldfish (Carassius auratus), and to determine the criteria necessary for the development of these infections.

The results indicate that water in the fish tanks must be slightly acid (pH 6), warm (22°C), nonmoving, and that the fish must be wounded in order for fungal infection to occur. Cool water (18°C) and moving water, caused by aeration and filtration, are detrimental to fungal colony …

The Great Potato Debate, James D. Hudson

Honors Theses

Anencephaly and spina bifida cystica are two of the most common severe congenital malformations consistently observed in human populations. Both represent defects in the neural tube and share a significant number of similar epidemiological associations among them, occupational class, geography, sex ratio, maternal age, year and season of the year. In anencephaly most of the brain and upper skull never form usually resulting in death within a few hours of birth. Spina bifida cystica is a malformation of the spinal cord often causing paralysis, vulnerability to infection, and early death. In 1972, Dr. J.H. Renwick presented a controversial hypothesis which …

Physiology Of Sporeforming Bacteria Associated With Insects: Minimal Nutritional Requirements For Growth, Sporulation, And Parasporal Crystal Formation Of Bacillus Thuringiensis., Kenneth W. Nickerson, Lee A. Bulla Jr.

Papers in Microbiology

A defined medium is described in which 18 strains of Bacillus thuringiensis representing the 12 established serotypes grow, sporulate, and produce a parasporal crystal. This minimal medium contains glucose and salts supplemented with either aspartate, glutamate, or citrate. These organic acids are required and cannot be replaced by vitamin mixtures or succinate even though succinate is taken up at a rate similar to that of aspartate, glutamate, and citrate.

Physiology Of Sporeforming Bacteria Associated With Insects: Radiorespirometric Survey Of Carbohydrate Metabolism In The 12 Serotypes Of Bacillus Thuringiensis, Kenneth W. Nickerson, Grant St. Julian, Lee A. Bulla Jr.

Papers in Microbiology

Radiorespirometry was used to compare the primary pathways of glucose catabolism in 18 strains of Bacillus thuringiensis representing the 12 established serotypes. Every strain utilizes the Embden-Meyerhof-Pamas pathway almost exclusively; pentose-phosphate pathway participation is minor. The Embden- Meyerhof-Parnas pathway predominates regardless of whether the cells were grown in a minimal medium or one containing yeast extract. The results indicate that the absolute requirement for citrate and related compounds is not a result of defective citrate or glucose transport and metabolism.

Purification And Characterization Of Syringacin 4-A, A Bacteriocin From Pseudomonas Syringae 4-A, William L. Haag, Anne K. Vidaver

Department of Plant Pathology: Faculty Publications

Syringacin 4-A, a bacteriocin produced by Pseudomonas syrinagae 4-A, was obtained by induction with ultraviolet irradiation or mitomycin C. Approximately 1,000-fold purification of the bacteriocin was achieved by manganous chloride precipitation, differential centrifugation, and chromatography on hydroxyapatite columns. The purified syngacin was homogeneous on hydroxyapatite columns and sucrose density gradients; it also sedimented as a single entity in the analytical ultracentrifuge. The buoyant density of purified syringacin in cesium chloride was 1.294 g/ml. The sedimentation coefficient was calculated as 120S, and the diffusion coefficient was 6.49 x 10^-8 cm²/s. The molecular weight was calculated as …

Primary Pulmonary Hypertension - Disease Or Symptom? A Retrospective Reclassification Of 33 Cases, D. S. Dasgupta, Ellet H. Drake

Henry Ford Hospital Medical Journal

The hypertension which follows an intrinsic increase in pulmonary vascular resistance may be easily diagnosed by utilizing current catheterization and radiologic techniques. The real challenge is establishing a rational etiology in a given case before final pathological examination. Data from 33 proven cases are presented in an attempt to show that most ofthe proposed etiologic concepts may be represented in a large clinical series. Congenital and familial factors, multiple pulmonary embolization with resulting vasospastic sequela, arteritis, and degenerative states, were encountered, together with such specific clinical entities as scleroderma and systemic lupus erythematosus. Because the term "Primary Pulmonary Hypertension" implies …

Malignant Intrathoracic Hurthle-Cell Tumor: Report Of A Case With 12-Year Survival Following Three Palliative Operations, Conrad R. Lam

Henry Ford Hospital Medical Journal

A 55-year-old man had a large superior mediastinal tumor producing obstructive symptoms. The tumor was removed through an upper median sternotomy incision. It was found to be composed of so-called Hurthle-cells, which are of thyroid origin, and the pathologist's interpretation was that it was of low grade malignancy. A pulmonary metastatic nodule was excised five years later, and 8 1/2 years after the original operation, a nodule was removed from a pectoral muscle. The patient lived 12 years and then expired with multiple metastases, but he was relatively asymptomatic until the last few months.

Implications For Microtubule Mediated Ethylene Effects In Pea Stem Sections, David A. Steen

Loma Linda University Electronic Theses, Dissertations & Projects

The marked effects of ethylene on pea stem growth have been investigated. Low temperature mid colchicine. both known microtubule depolymerization agents, reverse the effects of ethylene in straight growth tests. Low temperature (6 C) also profoundly reduces the effects of the gas in terms of swelling, hook curvature, and horizontal nutation. Electron microscopy shows that microtubules are reoriented after treatment with ethylene for as little as 12 hours. The findings indicate that some of the ethylene responses may be due to a stabilizing effect on microtubules in plant cells.

The Deoxythymidine Kinase-Deoxythymidylate Kinase Enzymes In Salmonella Potsdam And Escherichia Coli K12 Infected With Bacteriophage P3, James D. Kettering

Loma Linda University Electronic Theses, Dissertations & Projects

Metabolic alterations in bacterial hosts caused by virus infections have been studied for the last thirty years. The virulent T-even bacteriophages have been studied the most and have been shown to be responsible for the induction of a number of new, virus-specific enzymes that are necessary for successful phage growth. In addition, the activity of other host enzymes present prior to infection has been found to be increased by T-even infection. Temperate bacteriophages, being more difficult to work with than the virulent viruses, have not been studied as extensively. This is especially true for metabolic studies, since most temperate phage …

Lesion Formation In The Liver Of Mice Caused By Metabolic By-Products Of Hymenolepsis Microstoma, Gary Simpson

Masters Theses & Specialist Projects

The presence of lesions in the liver of mice is associated with the infection of these mice with the common bile duct -dwelling cestode, Hymenolepis microstoma. Lesions develop even though there is no direct contact between the cestodes and the liver. It was found in this investigation that administration to mice of Tyrode's solution, in which cestodes had been incubated for 24 hr, resulted in liver lesions comparable to those observed in normal infections. The administration of the causative substance by either per os intubation or intraperitoneal injection produced similar lesions. Further studies revealed that the active lesion - …

The Effects Of The Toxins Of Lophopodella Carteri (Ectoprocta) On The Blood Gas Properties Of The Bluegill Lepomis Macrochirus, Robert Allen May

Master's Theses

Lepomis macrochirus (bluegill) were exposed, in small containers, to homogenates of Lophopodella carteri, an ectoproct that contains substances toxic to gilled vertebrates. Blood pH, pco₂, and po₂ were determined with a blood gas analyzer. Blood carbonic anhydrase activity was measured manometrically.

The ectoproct toxins caused significant decreases in blood pH and po₂ and a significant increase in blood pco₂ and carbonic anhydrase activity. It is proposed that these changes in blood properties were initiated by a film of mucus covering the gill epithelium. The secretion of mucus is thought to be a response to irritation …

Characterization Of Selected Bacteria From The North Arm Of The Great Salt Lake, John L. Crane Jr.

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Thirteen bacterial cultures were isolated from the North arm of Great Salt Lake during the months of January and February of 1973. Eleven isolates were gram-negative pleomorphic rods which lysed in hypotonic solution. The remaining two were gram-positive cocci. All isolates and one known strain of Halobacterium salinarium were subjected to examination of morphological, cultural, physiological, and biochemical characteristics. A numerical taxonomic analysis was applied to the compiled characters to compute a coefficient of similarity for each individual isolate as compared to all other isolates. A comparative analysis was included in the similarity computation using characters assembled from those reported …

Effects Of X-Ray Induced Achlorhdria On The Utilization Of Dietary Calcium And Iron From Different Salts By Rats, Reid Scott Holbrook

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Dietary calcium has been classified as one of the minerals frequently limiting in the American diet (FAO, 1962) . Bone demineralization has been observed as a result of calcium deficient diets (Salomon et al., 1972), partial gastrectomies (Eddy, 1971) and inadequate hormonal balance (Albright et al., 1948). Although bone demineralization, or osteoporosis, has been attributed to many factors, the interrelationship of gastric acidity and the utilization of dietary calcium may be a key to the etiology of osteoporosis.

Eighty weanling male albino rats were divided into eight groups. All animals had their stomachs exposed through a mid-line incision. Control rats …

The Development Of An Index Of Food Quality, Ann Woolley Sorenson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The purpose of this study was to develop a food quality index that can provide practical information to both consumers and professional people. The Index of Food Quality (IFQ) is a simple computerized tool based on a concept of nutrient density. The IFQ was designed to organize and clarify the present body of food composition data into a form that is directly applicable to dietary problems.

The IFQ provides a method of analyzing food quality based on energy needs. The nutrient contents of foods are compared to essential nutrient standards with calories as a common denominator. In developing the IFQ, …

Abstracts Of Papers Scheduled For Presentation At The 86th Session Of The Iowa Academy Of Science, April 19-20, 1974, Iowa Academy Of Science

Iowa Academy of Science Documents

Presentation abstracts from the annual meeting of the Iowa Academy of Science

Fractionation Of Biologically Active Nucleic Acid Extract Of Volvox Aureus, Lucy Ann Gresham

Theses & Honors Papers

The colonial flagellate Volvox has been under study recently because its pattern of differentiation is simple and because it synthesizes chemical substances that induce or influence specific stages in its development (Darden, 1970; Starr , 1970). Volvox shows cellular differentiation and division of labor but has a maximum of four cell types, making Volvox's cellular diversity among the least complex of multicellular organisms . Chemical induction systems for males, females, and parthenospores are known for several species.

Male differentiation in Volvox aureus M5 is controlled by a male inducing substance (MIS) obtained from filtrates of sexual cultures (Darden, 1966). MIS …

Studies With An In Vitro Protein Synthesizing System Derived From Acetobacter Aceti Var. Liquefaciens, Joyce I. Cialdella

Masters Theses

No abstract provided.

Studies On The Membrane Associated L-Malate Dehydrogenase-Oxidase Complex Of Micrococcus Lysodeikticus, Jerry James Johnson

Masters Theses

No abstract provided.

Aspects Of Salt Tolerance Of Salicornia Pacifica Standl. Var. Utahensis (Tidestrom) Munz, Dennis J. Hansen

Theses and Dissertations

Aspects of salt tolerance of Salicornia pacifica were investigated. Survival of this plant in a saline environment is due to many interacting factors. A soil pH of 7.5 to 8.0 +- 0.2 and a constant sub-surface soil moisture level of 25 to 35% are necessary for plant survival. A fluctuating lower soil moisture leevel and higher pH favor etablishment of Distichlis stricta. The ion content in S. pacifica remained constant despite increased moisture stress throughout the growing season due to accumulation of salts which were significantly higher in the surface soil layers than in the subsurface layers around the roots. …

A Taxonomic Study Of Members Of The Family Entrobacteriaceae With Gas Chromatography, Madeline Ducate

Biological Sciences Theses & Dissertations

Abstract unavailable.

A New Transport Medium For Neisseria Gonorrhoeae, Nissan Altstock

Biology ETDs

A new transport and holding medium, designated medium #505, has been formulated and tested against standard, commercially available media. The medium is a chemically defined mixture. The ingredients include: sodium chloride, sodium thioglycollate, buffered agar, Amberlite CG-400, methylene blue and IsoVitalex.

The experimental design included two phases. During Phase I, transport media were formulated and tested for their ability to maintain a viable culture of the non-pathogen Neisseria perflava for at least 21 days at ambient temperature (25° C). In Phase II, media that were able to maintain Neisseria perflava for at least 21 days were tested for their ability …