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Mif-Like Activity In Non-Stimulated And Virus-Infected Cell Cultures, Eve Welling Brown

MUSC Theses and Dissertations

Macrophage migration inhibition factor (MIF) is a lymphocyte-derived substance which plays an important role in cell mediated immunity. Soluble factors containing MIF-like activity and produced by non-stimulated and virus-infected non-lymphoid cells have also been reported. In the present study, a MIF-like factor was repeatedly detected in Buffalo green monkey kidney cells infected with mumps or herpes simplex virus (HSV) indicating that this substance is reproducible and can be stimulated by a RNA and a DNA virus. Wistar-38 (WI-38) cell cultures also increased production of this substance in response to mumps but not HSV infection, indicating that the production of this …

Non-Histone Nuclear Proteins : Patterns Of Synthesis And Phosphorylation During The Cell Cycle, Redistribution At Times Of Gene Activation And Properties Of Subsets Interacting With Hnrna, Jonathan Karn

Student Theses and Dissertations

The interphase nucleus contains large numbers of nonhistone proteins, the majority of which are associated with chromatin. The amount and number of these proteins varies according to cell type. Actively proliferating cells with high transcriptional activity contain considerably more nonhistone nuclear proteins than do quiescent cells. In every cell type, the nuclear proteins are heterogeneous ; recent estimates based on two-dimensional gel electrophoresis place the total number of different HeLa cell chromosomal proteins at over 470 (Peterson and McConkey, 1976). Many of these proteins exhibit enzymatic activities associated with nuclear functions (such as DNA synthesis and repair, RNA synthesis, post-synthetic …

Regulation Of Expression Of The Bacteriophage T4 Genes 45, 44, And 62, Margaret G. Bowles

MUSC Theses and Dissertations

The functions of T4 genes 45, 44, and 62 are required for phage DNA replication. Some evidence suggests that in T4-infected E. coli these genes are cotranscribed in the 45 to 44 to 62 direction. In this study regulation of expression of genes 45, 44, and 62 has been examined by using SDS-polyacrylamide gel electrophoretic assays to analyze the protein products synthesized in T4-infected E. coli at various times after infection under different conditions. Genes 45, 44, and 62 were shown to be cotranscribed by the criterion of translational polarity, but the degree of polarity shown by gene 44 nonsense …

The Interaction Of Phenanthridinium Derivatives With Nucleic Acids, Antonis Kindelis

Dissertations

No abstract provided.

Informe Sobre Los Resultados Del Programa De Investigacion De Los Recursos Pesqueros Del Lago De Nicaragua, Instituto De Fomento Nacional

Investigations of the Ichthyofauna of Nicaraguan Lakes

E1 Instituto de Fomento Nacional (INFONAC), basado en los principios de su Ley Creadora y en la politica de desarrollo pesquero que se ha trazado, tome en el mes de Enero de 1971, la firme determinacion de iniciar el "Programa de Investigacion de los Recursos Pesqueros del Lago de Nicaragua" con una duracion estimada de tres (3) aiios, teniendo como base de operaciones la Ciudad de Granada.

An Investigation Into The Use Of Artificial Habitats As A Means Of Increasing The Fishery Productivity Of The Great Lakes Complex Of Nicaragua, Terrance M. Lim, Kenneth R. Mckaye, Douglas J. Weiland

Investigations of the Ichthyofauna of Nicaraguan Lakes

Protein deficiency is a major dietary problem in Central America where protein-rich foods are scarce and costly. Fish, however, represents a rich source of animal protein that is not being utilized to a significant extent. Moreover, it is a source that could be made available to large portions of the populations of these countries if improvements of the present fishing and distribution methods were realized. Prerequisite to expansion, however, is a basic understanding of the ecosystems and their components. It is at this preliminary stage that the situation presently lies. Once this understanding has been achieved, means of maximizing production …

Variación, Coloración Y Estado Sistemático Del Pez Centroamericano Cichlasoma Nicaraguense (Familia Cichlidae), Myrna I. López

Investigations of the Ichthyofauna of Nicaraguan Lakes

Cichlasoma balteatum and C. spilotum are placed in the synonymy of C. nicaraguense on the basis of a review of the literature and examination of fresh material from Lake Nicaragua and the Atlantic slope of Costa Rica. Observations on coloration and caudal fin scalation are included.

The Status Of The Freshwater Shark Of Lake Nicaragua, Thomas B. Thorson, Donald E. Watson, C. Michael Cowan

Investigations of the Ichthyofauna of Nicaraguan Lakes

Nineteen sharks collected from freshwater at several points on Lake Nicaragua and the Rio San Juan were all of the same species and indistinguishable from marine Carcharhinus leucas of the Atlantic. Minor differences previously thought to separate C. leucas from the lake shark proved to be invalid. Females had slightly longer gill slits, somewhat greater breadth of pelvic fin, and a longer abdominal region than males. Clasper length indicated that the onset of sexual maturity in males occurs when they are between 1,600 and 1,700 mm in total length.
The classical theory or origin of the sharks from Pacific ancestry …

Comparison Of Some Parameters Of Serum And Uterine Fluid Of Pregnant, Viviparous Sharks (Carcharhinus Leucas) And Serum Of Their Near-Term Young, Thomas B. Thorson, Jeffrey W. Gerst

Investigations of the Ichthyofauna of Nicaraguan Lakes

I. In eight females, serum urea concentrations of 134-336 mM/l. were interpreted as indications of previous environmental salinities.
2. Fetal serum solute concentrations usually resembled those of the mother, but in two cases where fetal urea was higher, it was interpreted as a lag in urea reduction following movement from salt to fresh water.
3. Uterine fluid was similar to maternal serum in all parameters except for its very low protein content.
4. No flushing of the uteri with environmental water occurs.
5. Independent osmoregulation apparently does not occur before birth, although required mechanisms may be present.
6. The full …

The Rectal Gland In Relation To The Osmoregulatory Mechanisms Of Marine And Freshwater Elasmobranchs, Giuseppe Gerzeli, Gian Franco De Stefano, Lorenzo Bolognani, Kurt W. Koenig, Maria Victoria Gervaso, Maria Fausta Omodeo-Salé

Investigations of the Ichthyofauna of Nicaraguan Lakes

The rectal gland of elasmobranchs secretes large quantities of electrolytes and is of primary importance in the osmoregulatory mechanisms of these animals (Burger and Hess, 1960; Burger, 1962). Analogously, various organs of other vertebrates are also known to be involved in salt secretion (Schmidt-Nielsen, 1965; Bonting, 1970; Bentley, 1971). In general, active ion transport is the basis of this phenomenon; in any case, it requires the presence of structural devices, morphologically and chemically well defined, and of metabolic capabilities which, taken singly, are not specific, but collectively are consistent with active ion transport and salt secretion.

Effect Of Soil Buffer Capacity On Soil Reaction (Ph) Modification And Subsequent Effects On Growth And Nutrient Uptake Of Plantanus Occidentalis L. Seedlings, P. E. Pope, R. B. Vasey

Journal of the Arkansas Academy of Science

The buffer capacity of a soil is a significant factor in determining the longevity of soil reaction (pH) adjustments by aluminum sulfate, Al2(SO4)3, or calcium carbonate, CaCO₂. After 12 weeks the modified pH values of the highly buffered Emory silt loam had changed substantially toward the original pH value of 7.6. Modified pH values for the Groseclose silt loam soil remained essentially unchanged under the same conditions. These differences in soil response to modified soil pH are related to the differences in the percentage of vermiculite chlorite and chlorite in the clay fractions of the two soils. The longevity of …

Hormone Receptor Site Maturation In The Secondary Sex Organs Of Immature Male And Female Rats, K. J. Thomas

Journal of the Arkansas Academy of Science

The effect of a combined dose of pregnant mares' serum (PAAS) and human chor ionic gonadotropin (HCG) on male and female rats 2-25 days old was studied. Groups of animals were given injections for three days, then sacrificed on the fourth day. All injections were begun on the 2nd, 7th, 12th, 17th, and 22nd day of life. Ovaries, uteri, seminal vesicles (SV), and ventral prostate (VP) were removed, dissected free of fat, and weighed. Because uterine weight increased earlier than either SV or VP weight, the ability of the uterus to respond to exogenous estradiol and of the SV and …

Physiologic And Embryologic Studies Of The Yellow (Ay/A) Mouse, Gerald R. Cizadlo

Electronic Theses and Dissertations

Mice heterozygous for the dominant AY gene have a yellow coat plus an altered metabolism which results in diverse physiologic characteristics including a greatly increased amount of carcass fat, a longer body and tail length, slightly more muscle mass, increased feed efficiency, increased susceptibility to spontaneous and induced tumor growth, and reproductive inefficiency in females. Homozygous AY animals die in utero. The mutation produces a lesion which is potentially valuable as an investigative· tool for the study of physiologic mechanisms and relationships of diverse metabolic pathways. Several experiments were performed to gain information concerning the AY effect. Rate of oxygen …

Azide Sensitivity In Species Of Chlamydomonas And Carteria, Kim Michael Kobriger

Masters Theses

No abstract provided.

Land Snail Diversity At Rocky Branch Nature Preserve, Clark County, Illinois, Daniel J. Mott

Masters Theses

Terrestrial, shell-bearing gastropods were collected at Rocky Branch Nature Preserve, Clark County, Illinois. A total of three hundred nineteen specimens were collected representing five families and fifteen species. Two species of the family Endodontidae, eight species of the family Polygyridae, three species of the family Zonitidae, and one species in each of the families Pupillidae and Succineidae were collected.

In addition, an attempt was made to find the relationship between species diversity and dominant woody vegetation and soil quality. The land at Rocky Branch Nature Preserve ranges from lowland flood plain to high second growth forest. The dominant trees of …

April Foods Of The Wild Turkey In Southern Illinois, David K. Fleenor

Masters Theses

A total of 95 gizzards and 106 crops were collected from 120 wild turkeys (Meleagris gallopavo silvestris Vieillot) at hunter check stations located in Alexander, Jackson, Union, and Pope Counties during April 1970, and 1972-1974 inclusive. Results are presented using total aggregate volume and per cent occurrence. The analyses indicated that during April, 96.6% of the foods eaten were of plant origin. Important plant foods in decreasing order by volume included corn, acorns, grasses and sedges, clover, miscellaneous plant materials, Jack-in-the-pulpit, hickory nuts, black gum, swamp buttercup, spring beauty, soybeans, hill blueberry, hawthorn, and ash. Animal foods were …

Louisiana Agriculture Magazine, Winter 1976-1977, Lsu Agricultural Experiment Station

Louisiana Agriculture

No abstract provided.

Purine-Thiamine Anabolism During Cellular Morphogenesis In Myxococcus Xanthus, Wen-Cherng Tsai

Electronic Theses and Dissertations

Purine-thiamine anabolism during cellular morphogenesis in Myxococcus xanthus was investigated. One of the approaches was to test vegetative and myxospore (2.5 h and 8 h) extracts of M. xanthus CW-2 for their ability to synthesize the second de novo intermediate, 5'-phosphoribosylglycinamide, from beginning precursors either by way of phosphoribosyl-pyrophosphate amidotransferase (EC 2.4.2.14) or ribose-5-phosphate aminotransferase. Results indicate the presence of both the amido- and aminotransferases in both types of extracts, and both enzymes appear to be present at about the same level (per milligram of protein) in vegetative cells, myxospores (2.5 h and 8 h), and in a bacterial prototype, …

580 Extension Plan: 5 Year Plan For Extension & Research By 1980 For Southeast Nebraska, Loyd L. Young, Elizabeth A. Birnstihl

Southeast Research and Extension Center: Reports and Administration

The purpose of this document is to present a series of guide-lines to assist the Extension District V (Southeast Extension District) administrators and staff, the Nebraska Cooperative Extension Service, the Nebraska Agricultural Experiment Station, the Institute of Agriculture and Natural Resources and other University administration in moving the District to a high level of 0fficiency in meeting the needs of the people of the District in those areas in which the Extension Service has the legal obligation to serve.

Planning is a continuous process and these planning suggestions are not ends, but steps that can be used in decision making …

Upward Water Movement In Field Cores, Larry G. Wells, R. W. Skaggs

Biosystems and Agricultural Engineering Faculty Publications

No abstract provided.

Progress In The Nursery Industry, W J. Heggers

Journal of the Department of Agriculture, Western Australia, Series 4

The demand for ornamental plants in Western Australia is sat an unprecedented level, making the nursery industry a relatively prosperous branch of horticulture.

One reason for the continued demand for nursery lines is probably that people are concerned about the environment - "green survival", as it is often refered to in promotional literature.

In rural areas, farmers are becoming increasingly aware of the value of planting windbreaks, shade and ornamental trees, while mining companies and the Forests Department are actively engaged in re-afforestation.

As a result the nursery industry is in a healthy situation and can continue to develop in …

Mercury Levels In Muscle Tissue Of Stream-Dwelling Fish, Tom Frost

Masters Theses

No abstract provided.

The Effects Of Las On The Ultrastructure Of The Teleost Chorion, Mark Xavier Vancura

Master's Theses

No abstract provided.

Photoevoked Response Of The Optic Tectum In Larval Stage Xii And Juvenile Rana Catesbeiana, Michael Gale Phillips

University of the Pacific Theses and Dissertations

The previous cytological and electrophysiological work suggests that the optic tectum matures during metamorphic climax. It was the aim of the present work to attempt to compare pre metamorphic (Stage XII) larval and post metamorphic juvenile, Rana catesbeiana, using a photostimulated evoked electroencephalographic (E.E.G.) response in the optic tectum and correlate that with tissue studies to verify neurophysiological maturation of the optic tectum.

The Antibiogram As An Aid In The Identification Of The Klebsiella-Enterobacter-Serratia Group, Richard Keith Hall

University of the Pacific Theses and Dissertations

The purpose of this study was to examine and evaluate the K-E-S group from this community with respect to distribution in clinical materials, detailed individual biochemical characteristics, and antibiogram patterns using the Bauer-Kirby disc technique, and compare these findings with those of other investigators from other geographic locations. As far as can be determined, this is the only study of its kind on the West Coast of the United States.

The Ecology Of Some Harpacticoid Copepods Of A California Sand Flat, David R. Williams

University of the Pacific Theses and Dissertations

The main purpose of this study is to describe the population ecology of eight species of harpacticoid copepods over a period of one year with emphasis on the effects of physical interactions and biotic factors on these populations. The species were: Amphiascopsis sintus (Claus) 1866, Stenhelia (D.) oblonga Lang, 1965, Heterolaphote variables Lang, 1965, Ectinosoma melaniceps Boek ? var. T. Scott, 1902, Paralophonte subterraneas Lang, 1965, Tisbe furcata (Baird) 1837, Diasaccus spinatus Cambell, 1929 and Parathelestris bulbiseta Cambell?.

The Life Cycle Of Seven Species Of Drosophila, Miriam Marquez Marin De Flores

University of the Pacific Theses and Dissertations

The purpose of this work is to determine the life cycle of different species of Drosophila of different subgenera and coming from different environments.

The Functional Development Of The Adrenal-Sympathetic Nervous System In Neonatal And Adolescent Swine, Sidney Koon Hung Woo

University of the Pacific Theses and Dissertations

The development of the animal after birth is marked by complex changes. Certain periods are particularly important and have been defined as "critical periods" (Krecek, 1971). Two processes are important to the survival of the animals during these times. The process of "adaptation" involves the maturation of specific physiological systems according to the needs of the neonatal animal, and is dictated by the external environment. The process of "tolerance" renders the neonate relatively unresponsive to a stressor as compared to the adult and protects the animals from over-responding the depleting vital resources until it is physiologically mature. An example of …

Efficacy Of Bunamidine Hydrochloride Against Immature And Mature Stages Of Echinococcus Granulosus, Lauritz A. Jensen

Theses and Dissertations

A study was conducted to determine the efficacy of bunamidine hydrochloride against the immature and mature stages of Echinococcus granulosus found in Utah. Twenty-seven dogs were obtained from local animal shelters and experimentally infected with the parasite. The drug was given at dosage levels of 25 mg/kg to 50 mg/kg of bodyweight. It was from 85.9 to 98.8% effective against the immature stage, and 100% effective against the adult stage. The results suggest that this drug can feasibly be used under field conditions where hydatid disease is endemic.

Ultraviolet Radiation Effects On Rotifers, Syed Asim Hussain

Masters Theses & Specialist Projects

The purpose of this investigation was to study ultraviolet radiation effects on rotifers. The rotifer studied was Philodina acuticornis odiosa Milne. Isolation cultures were used to grow the rotifers individually in separate U-plate depressions. The source of ultraviolet radiation consisted of four mercury germicidal lamps used in combination to deliver a uniform flux of 315 uW/cm² over the sample. Five groups of rotifers, one control and four experimental groups of 96 each, were used. The rotifers used were 2-10 hours of age at the beginning of the experiment. The experimental groups were exposed to uv radiation for 3 minutes, …