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The Study Of The Pathology Of The Middle One Third Of The Small Intestine Of The Spontaneously Diabetic Chinese Hamster, Patrick Murray

Masters Theses

No abstract provided.

Seasonal Distribution Of Higher Marine And Aquatic Fungi In The Back Bay National Wildlife Refuge, Scott Schatz

Biological Sciences Theses & Dissertations

Abstract unavailable.

The Role Of Zinc In Dihydroorotase, Pamela S. Gilchrist

Dissertations and Theses

Dihydroorotase (4,4—dihydroorotic acid amidolyase, EC 3.5.2.3.) which catalyzes the reversible cyclization of N-carbamyl-L-aspartate to L-dihydroorotate has been purified from orotate-grown Clostridium oroticum. The enzyme is stable in 0.3 M sodium chloride and 10 µ ZnSO₄. Sodium dodecyl sulfate gel electrophoresis indicates the enzyme to be composed of two identical subunits each with a molecular weight of 58,000 + 6000. Dihydroorotase is shown to be a zinc-containing metalloenzyme with 2 g atoms of zinc per 58,000 g of protein. The role of zinc in dihydroorotase is discussed.

Lipid Metabolism During Bacterial Growth, Sporulation, And Germination: An Obligate Nutritional Requirement In Bacillus Thuringiensis For Compounds That Stimulate Fatty Acid Synthesis, Kenneth W. Nickerson, Lee A. Bulla Jr.

Papers in Microbiology

The regulation of fatty acid biosynthesis by compounds that are required for growth of Bacillus thuringiensis was investigated using an in vivo assay developed to measure fatty acid synthesis in germinating spores. A minimal glucoseammonium- salts medium does not support growth even though previous radiorespirometric studies have shown B. thuringiensis to possess intact tricarboxylic acid and Embden-Meyerhof-Parnas pathways. Abundant growth does occur, however, when this medium is supplemented with either glutamate, aspartate, citrate, thiosulfate, cystine, or ethylenediaminetetraacetic acid. Cells held under non-growing conditions incorporate acetate into fatty acids; fatty acid synthesis is stimulated by the compounds that permit growth. These …

Pollution Of Surface Irrigation Waters By Plant Pathogenic Organisms, James R. Steadman, C. R. Maier, H. F. Schwartz, E. D. Kerr

Department of Plant Pathology: Faculty Publications

Systematic sampling of waterways and irrigation runoff from agricultural lands in the North Platte Project of Nebraska in July and August of 1972–1974 demonstrated that phytopathogenic organisms were disseminated. The organisms monitored included the bean common blight bacterium Xanthomonas phaseoli, the bean white mold fungus Whetzelinia sclerotiorum and various nematodes. Although many types of nematodes often were recovered from irrigation water, Heterodera sp. cysts which cause significant disease problems in the valley were found infrequently. Patterns of movement of the bacterial and fungal organisms were correlated with previous or current season infection of bean plants. The short-term survival of X. …

The Effects Of Certain Steroids On The Growth Of Slime Mold, Physarum Polycephalum, Ching-Bun Paul Chen

Theses and Dissertations

The cortex of vertebrate adrenal glands secretes two major groups of hormones--glucocorticoids and mineralocorticoids. Glucocorticoids, which have an oxygen atom at carbon number 11, increase the rate of gluconeogenesis and increase the mobilization of lipids in mammals (Turner and Bagnara 1971). At the same time there is a decrease in protein synthesis. This latter effect may be homologous to a decrease in fibroblast growth in culture in the presence of certain corticosteroids (Ruhmann and Berliner 1965). The main function of mineralocorticoids, on the other hand, is to retain sodium ions. They have little or no gluconeogenic activity. Interestingly, however, these …

The Role Of The Suprapharyngeal Ganglia In Regulation Of Oxidative Metabolism In The Intestinal Mitochondria Of Lumbricus Terrestris, Raymond H. Fetterer

Masters Theses

No abstract provided.

Percent Accuracy By Crown Closure Classes Of Woodland Stands Mapped From Skylab-4 Photography And Landsat-1 Imagery, Roger Dale Goldsmith

Electronic Theses and Dissertations

LANDSAT-l imagery and satellite photography have been utilized for land-use mapping. This study investigated the correlation between woodland stands mapped and their percent crown closure using LANDSAT-l imagery and Skylab-4 photography. On the basis of percent accuracy of crown closure classes mapped or not mapped, Skylab-4 photography proved superior over LANDSAT-l imagery for mapping woodlands

Timpanogos Flora, Kelly W. Allred

Theses and Dissertations

A comprehensive floristic study of Mt. Timpanogos, Utah County, Utah, was undertaken. The Mt. Timpanogos area is a much used facility in recreation, biology, watershed development, livestock management, and education. It is also representative of a major segment of the overall flora of Utah. This study adds to the ever-increading knowledge of the flora of Utah, and provides a means of identifying the flora of this region, which is basic to any future management.

Effects Of Antifibroblast Antiserum On Cells Derived From Fibroblast Outgrowth Of Human Prostatic Tissues, Eva Shang-Lian King

Dissertations and Theses

The purpose of this investigation was to provide pure cultures of normal human prostatic epithelium free of fibroblasts in order to study malignant conversion by chemical carcinogens. Normal epithelial cells were needed because this was the cell type implicated in prostatic malignancies of human subjects. Unfortunately fibroblasts grew faster than epithelial cells so that cultures were always overgrown with connective tissue elements. It was considered important to find a method which would eliminate fibroblasts so that normal epithelial cells could grow out in pure culture.

A Preliminary Survey Of The Vascular Flora Of Upper Tygarts Creek, Carter County, Kentucky, William Michael Huffaker

Morehead State Theses and Dissertations

A thesis presented to the faculty of the School of Sciences and Mathematics at Morehead State University in partial fulfillment of the requirements for the Degree of Master of Science in Biology by William Michael Huffaker on July 23, 1975.

Louisiana Agriculture Magazine, Summer 1975, Lsu Agricultural Experiment Station

Louisiana Agriculture

No abstract provided.

Sodium Bentonite In Ruminant Rations, Daniel P. Colling

Open Access Master's Theses (through 2010)

Sodium bentonite, an expanding clay, is being used in many different ways in industry today.The livestock and feed industry, for example, use it in many different ways.It is used as a pellet binder to maintain a fat test in dairy animals, as a digestive aid in the poultry industry and as a “buffering agent” in feedlot rations.

A two-pronged investigation was undertaken to determine the effect sodium bentonite could have on the ruminant feeding industry.One part of the investigation sought to determine if bentonite could partially or entirely alleviate subclinical lactic acidosis with problem rations.The other part of the investigation …

Nepal Studies Association Bulletin, No. 8, Nepal Studies Association, Donald A. Messerschmidt

Nepal Studies Association Newsletter

No abstract provided.

The Bacteriology Of The Biliary Tract: A Preliminary Report., J. A. Sapala, J. L. Ponka, T. R. Neblett

Henry Ford Hospital Medical Journal

The bacterial flora of acute biliary tract disease has not been completely elucidated. The organisms identified most often were coliforms. Anaerobic culture technics were not precise enough to provide proper identification. In a 12-month study of 250 patients operated upon for inflammatory gallbladder disease, culture specimens were collected in a most strict and rigorous fashion. It was hoped that a more exact understanding of the relationship between bacteria and inflammatory biliary tract disease might be appreciated. Important technics were developed for gathering specimens, delivery to the laboratory and culturing. More meaningful results are to be expected In the future.

Leaf Freckles And Wilt Of Corn Incited By Corynebacterium Nebraskense Schuster, Hoff, Mandel, Lazar, 1972, M. L. Schuster

Nebraska Agricultural Experiment Station: Historical Research Bulletins

The new bacterial disease Leaf Freckles and Wilt (LFW) of corn (Zea mays L.) was first observed in 1969 on two farms in southcentral Nebraska. Since then it has spread to other areas in the state. LFW is a serious disease on farms in six Nebraska counties (Clay, Custer, Dawson, Furnas, Hall and Phelps) and has been found in isolated cases in at least 28 other counties. In 1971 LFW was found in a seedcorn field in western Iowa, in 1973 in two Kansas counties, and in 1974 in South Dakota and Colorado. Because of its explosive nature, several …

The Metabolism Of Tartaric Acid By Aspergillus Ficuum, Larry Daniel

Masters Theses & Specialist Projects

Soil samples were screened for organisms which decompose tartaric acid as an energy source. Several microorganisms were isolated which were capable of metabolizing tartaric acid. The organism which grew best was identified as Aspergillus ficuum and used in this study. This organism, when grown in tartrate medium, resulted in a decrease in optical rotation and density of the culture medium. The pH of the culture filtrate increased with a concurrent increase in mycelial weight. Analysis of culture filtrates by gas chromatography indicate that the first intermediate compound detected in tartrate decomposition by A. ficuum is glycolic acid. The terminal metabolite …

Manufacture Of Cottage Cheese Utilizing A Ph-Controlled, Whey-Based Lactic Culture, Yen-Luon Chen

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Eleven strains of lactic cultures were used to determine their effectiveness in different bulk culture media for the manufacture of Cottage cheese. The acid phosphate-treated whey-based medium (APWM) made from Cottage cheese whey was found to be as effective as sweet phosphate-treated whey-based medium (PWM) made from Swiss cheese whey in supporting the development of lactic cultures. The relative activity of cultures in APWM with pH control was higher than phage inhibitory medium and nonfat dry milk without pH control. APWM was used successfully in the manufacture of Cottage cheese. Greater reliability and shorter cutting time resulted, and the product …

Comparison Of Compatibility Of Streptococcus Starters In Reconstituted Nonfat Dry Milk And A Whey-Based Bacteriophage Inhibitory Medium, Clinton K. Searle

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Compatibility of mixed cultures of Streptococcus lactis and Streptococcus cremoris were studied by the phage tracer technique in 10% nonfat dry milk and a whey-based phage inhibitory medium. Methods of membrane dialysis and differential enumeration were tried and abandoned. Dominance occurred in nonfat dry milk and in the whey-based medium during a continuous fermentation period within a pH range 6.8 to 5.2. The dominance pattern of two-strain combinations in 10% nonfat dry milk was different from that in the whey-based media. Dominance varied from one strain to another in no consistent sequence in some combinations. Dominance patterns of combinations were …

Evaluation, Assay, Distribution, And Survey Of Staphylococcus Aureus Thermonuclease In Milk And Cheese Products, Gene Leong Hong

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The metachromatic agar-diffusion test of Lachica, Hoeprich, and Genigeorgis (1971) was modified by adding 17 ml of the agar medium to a 100 by 15 mm plastic petri dish, using 5 µl samples and 3 mm wells. The test detected 0.5 ng/ml of thermonuclease in 3 hr at 37 C. This was equivalent to 10⁶ to 10⁷ cells/ml of Staphylococcus aureus strains 196 E, 14458, and 19095 grown in Brain Heart Infusion (BHI) broth. The test sensitivity was decreased ten fold in raw and pasteurized milk.

One-half gram of cheese was dissolved in 4.5 ml of 0.1 M …

The Effects Of Gamma Irradiation, Color Mutation And Various Subatmospheric Pressures On The Growth And Patulin Production By Penicillium Expansum And Penicillium Patulum, Karl B. Adams

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

To assess the effects of gamma radiation at low doses, spores of Penicillium expansum NRRL 971 and Penicillium patulum NRRL 989 were exposed to doses of 0, 50, 100, 150, and 200 krads. The amount of patulin for each culture was quantitatively determined and growth at each dose was evaluated. The results showed a 31% increase in the amount of patulin synthesis after a dose of 150 krads for Penicillium expansum. At 200 krads, both fungi showed low levels of patulin production and inhibited growth.

Eight color mutants of P. expansum and eight color mutants of P. patulum were produced …

Regulation Of Rabbit Testicular Capsular Motility: The Interaction Of Prostaglandins, Acetylcholine And Sympathomimetic Agents, James L. Hargrove

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The regulation of the contractility and intratesticular pressure of rabbit testes was studied. The effects of, and interactions among, prostaglandins (PGs) E₁ and F_2α testosterone, acetylcholine, epinephrine and norepinephrine were ascertained both in vitro and in vivo. All of these compounds, except for high concentrations of PGE₁ and testosterone, stimulated the testicular capsule to contract. PGE₁ at low concentrations potentiated the contractions produced by epinephrine and acetylcholine. Above 10^-7M, PGE₁ inhibited contractions caused by PGF_2α epinephrine or acetylcholine. Testosterone inhibited contractions caused by PGF_2α in vitro.

Preinjections of rabbits …

Carbon, Nitrogen And Algal Biomass In Cold Desert Soil Crusts, Mary Cleave Vogelsberg

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The algal biomass, total organic carbon, total nitrogen and percent soil moisture of soil crusts for a ten month period are presented for the Curlew Valley region of northern Utah. The estimates establish a significant relationship among these parameters.

A method involving chlorophyll extraction to determine the biomass of soil algae has been developed, and from this method the following conclusions are suggested: the revegetation of soil surfaces by algal crusts after lethal treatment, without physical disruption, appears to be rapid; there is no apparent relationship between the amount of algal biomass and the type of vascular vegetation present.

Detection Of Neonatal Calf Diarrhea Virus (Ncdv) And Human Infant Reolike Diarrhea Virus, Martin Wyatt Peterson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The purpose of this study was to develop a diagnostic test and conduct a survey for the neonatal calf diarrhea virus (NCDV) and human infant reolike diarrhea virus. Two immunologic methods were developed in this investigation.

Immune electron microscopy (IEM) and the fluorescent viral precipitin test (FVPT) are the methods used to detect NCDV and the human virus. Both methods are based upon the principle that viral aggregates form when the virus is reacted with anti-NCDV antibody. Aggregates in the IEM method are negatively stained and observed with the use of an electron microscope. Fluorescein labeled antibody is used in …

The Influence Of A Nutrition Education Program On Preschool Children, Nida Diane Houston

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

A study on the influence of nutrition education experiences was conducted in the Child Development Preschool Laboratory at Southern Utah State College in Cedar City, Utah, with thirty-two preschool children. Sixteen of the children were exposed to nutrition experiences through food activities and stories.

It was found that the sixteen children who were involved in the fifteen nutrition experiences significantly increased their knowledge of nutrition concepts and further modified their own personal food choices. The control group, which was not exposed to nutrition activities, made no significant change in knowledge of nutrition concepts and no modification of personal food choices. …

Abstracts Of Papers Scheduled For Presentation At The 87th Session Of The Iowa Academy Of Science, April 19, 1975, Iowa Academy Of Science

Iowa Academy of Science Documents

No abstract provided.

Farm, Ranch And Home Quarterly Institute Of Agriculture And Natural Resources, University Of Nebraska- Lincoln

Farm, Ranch and Home Quarterly

In this issue:

2 Inflation Fighters

5 Alfalfa in Swine Finishing Diets

7 Protein Levels for Swine

9 A Million Pounds of Education

11 Is Car Exhaust Poisoning Our Corn?

12 Nebraska's and Pasture Resources

13 Clothing Goals

16 Partners in Horticulture Therapy

19 Dry Bean Planting, Weather Or Not

20 Fertilizing Proso Millet

22 Agriculture in the Holy Land

Louisiana Agriculture Magazine, Spring 1975, Lsu Agricultural Experiment Station

Louisiana Agriculture

No abstract provided.

Limnological Investigation Of The Muskegon County, Michigan, Wastewater Storage Lagoons, W. Randolph Frykberg

Masters Theses

No abstract provided.

Folate Content Of Bacillus Subtilis, David Nelson Hintze

Theses and Dissertations

Bacillus subtilis can synthesize deoxythymidylate via either of two pathways. The working hypothesis is that the two reactions are very similar, differing only with respect to specificity for the type of folate required as substrate. The folates from B. subtilis were isolated and were determined by gel chromatography to be derivatives of pteroyltriglutamate and pteroyldiglutamate. Kav values for the two folate species are reported. Conjugase treatment revealed no other highly conjugated folates present. It was also observed that mutants deficient in one or the other of the two pathways grow at the same rate as wild-type cells under conditions allowing …