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Ninety Degree Tilt Module And Double-Sided Stub For Scanning Electron Microscopy, G. L. C. Mcturk

Scanning Electron Microscopy

A simple inexpensive device to achieve ninety degree tilt in some scanning electron microscopes (SEM) not equipped with this facility. A new method of safely mounting and studying both sides of the same fragile flat specimen, without the hazards of remounting; used in conjunction with this device.

Chromatid Behavior In Late Mitosis: A Scanning Electron Microscopy Analysis Of Mammalian Cell Lines With Various Chromosome Numbers, D. A. Welter, D. A. Black, L. D. Hodge

Scanning Electron Microscopy

Chromatid activity during the process of nuclear reformation following metaphase is a period of mitosis where little precise information is available. Nuclear reformation requires that chromosomes, at metaphase and chromatids during anaphase and telophase align, position and associate in a clearly defined sequence to insure the specific design of each nucleus. Four cell lines with chromosome numbers ranging from seven to almost seventy were chosen to determine whether the process of nuclear assembly is the same throughout. Chromosomal alignment at metaphase is found to be radial in all four cell lines. Chromosome positioning is essentially the same in all four, …

Santa Clara Magazine, Volume 29 Number 1, Fall 1986, Santa Clara University

Santa Clara Magazine

10 - IS SANTA CLARA STILL JESUIT? Declining vocations prompt this recurring question. Some of the faculty discuss what makes Santa Clara Jesuit to them. By Peg Major

16 - DON'T CALL ME MR. MOM Taking over the day care of his two tiny daughters while his wife worked provided this alumnus new insight on fatherhood. By Jim Craven

21 - PERSPECTIVES OF A PATRIARCH The spotlight is on Tom Bannan, class of '23, who started the procession of Bannans to Santa Clara. BY Paul Hennessy

26 - HOW NOT TO MAKE MONEY ON THE STOCK MARKET A guide to …

Revision Of The Nearctic Glyphicnemis Foerster (Hymenoptera: Ichneumonidae, Gelinae), John Luhman

Insecta Mundi

GLYPHICNEMIS Foerster is a small Holarctic genus in the subtribe Endaseina of the Gelini. Nearctic species were placed in PHYGADEUON Gravenhorst until Townes (1944) placed them in the subgenus GLYPHICNEMIS within ENDASYS Foerster . However, earlier Cushman (1925) had transferred PHYGADEUON CRASSIPES Provancher, a junior synonym of G. MANDIBULARIS (Cresson), to GLYPHICNEMIS, and the Palearctic species, at first placed in PHYGADEUON, were included by Habermehl (1916) i n GLYPHICNEMIS, reduced t o a subgenus within STYLOCRYPTUS Thomson (1873). This status was continued until Townes (1970) listed and described GLYPHICNEMIS as a seperate genus.

The Systematics And Biology Of The Flea Beetle Genus Crepidodera Chevrolat (Coleoptera: Chrysomelidae) In America North Of Mexico, Richard H. Parry

Insecta Mundi

Crepidodera Chevrolat is a genus of small metallic-colored flea beetles belonging to the family Chrysomelidae. Although these insects are quite common in the field and numerous in museum collections, the members of the genus in North America are, until now, poorly known. Heikertinger (1948-1950) recognized 4 taxa and recently, Lazorko (1974) described 3 additional species. These 7 species were recognized primarily on the basis of genital differences and were otherwise difficult to identify.

Examination of a large accumulation of museum material and investigations in the field have indicated the presence of several additional species in the North American fauna. A …

Louisiana Agriculture Magazine, Fall 1986, Lsu Agricultural Experiment Station

Louisiana Agriculture

No abstract provided.

The Effect Of Pre-Sterile Medium Ph Photoperiod And N6-(2-Isopentenyl)-Adenine (2ip) On Shoot Production During Stage Ii Micropropagation Of Rhododendrons, Lori Longo Smith

Biological Sciences Theses & Dissertations

The number of shoots produced during a six week subculture of two Rhododendron sp., cultivars Rocket and Gibraltar was the same for both 16 and 24 hour photoperiods under cool white fluorescent lights, whereas significantly fewer shoots were produced under a 10 hour photoperiod for ‘Rocket.' Increasing concentrations of N₆- (2-isopentenyl)- adenine (2iP) from 0 to 32 μMin Anderson's medium resulted in proportional increases in shoot number. When shoots were cultured in the presence of indole-3-acetic acid (IAA) and 2iP, shoot multiplication was increased significantly over shoots cultured with 2iF alone. No interaction affecting shoot multiplication was …

Analysis Of Mosquito Larvicidal Potential Exhibited By Vegetative Cells Of Bacillus Thuringiensis Subsp. Israelensis, Corey J. Walther, Graham A. Couche, Mary Ann Pfannenstiel, Sara E. Egan, Lisa A. Bivin, Kenneth W. Nickerson

Papers in Microbiology

Vegetative Bacillus thuringiensis subsp. israelensis cells (6 x 10⁵/ml) achieved 100% mortality of Aedes aegypti larvae within 24 h. This larvicidal potential was localized within the cells; the cell-free supernatants did not kill mosquito larvae. However, they did contain a heat-labile hemolysin which was immunologically distinct from the general cytolytic (hemolytic) factor released during solubilization of B. thuringiensis subsp. israelensis crystals. The larvicidal potential of the vegetative cells was not due to poly-3-hydroxybutyrate. Instead, it correlated with the ability of vegetative cells to sporulate during the bioassays. No toxicity was observed when bioassays were conducted in the presence …

Contribucion Al Conocimiento De Los Pepsinae Sudamericanos (Hymenoptera, Pompilidae) Iii. El Genero Priocnemis Schiødte En Chile, Arturo Roig Alsina

Insecta Mundi

El género Priocnemis SchiØdte no se puede considerar como caracteristico de la Región Neotropical y son pocas las especies descritas que se conocen de ella. Sin embargo en el área extra-tropical sudamericana se halla bastante bien representado por un grupo de especies que presenta características particulares, como veremos más adelante. Son varias las especies presentes en la Argentina, Uruguay y Chile, pero por el momento vamos a referirnosala fauna de este último país. Debemos aclarar que no consideramos aquí las especies correspondientes a Sphictostethus Kohl, que han sido tratadas como Priocnemis por algunos autores, ya que constituye un género bien …

Praestochrysis Of The Ethiopian Region With A Key And Descriptions Of New Species(Hymenoptera: Chrysididae), R. M. Bohart

Insecta Mundi

Praestochrysis Linsenmaier contains those species of Chrysidinae with five teeth on the posterior margin of tergum III, first flagellomere (F-I) less than 3 times as long as broad (often much shorter), and clypeal length below antennal sockets (subantennal distance) not more than diameter of the midocellus (MOD). The genus is widespread in the Old World, but a majority of the known species are in the Ethiopian Region. Host records are rather few, but it is likely that nearly all species attack moth larvae and emerge from their cocoons. Praestochrysis shanghaiensis (F. Smith) is a well known parasitoid of the silk …

Freeze-Dried Embedded Specimens For Biological Microanalysis, L. Edelmann

Scanning Electron Microscopy

The main problems associated with freeze-drying of biological material for electron microscopy concern the freeze-drying temperatures and times necessary to minimize artifacts. Due to the many parameters involved these problems have to be resolved experimentally. It can be shown that good morphological preservation of chemically unfixed material is possible when freeze-drying is done exclusively in a temperature range between -80°C and -50°C. OsO₄ vapour fixation of the freeze-dried tissue is not necessary and should be avoided because it may cause ion redistribution artifacts. Embedding at low temperature of properly freeze-dried material does not seem to disturb structure and ion …

Dissection Technique For Cochleas Prepared For Scanning Electron Microscopy, Sven Rydmarker, D. E. Dunn, P. Nilsson, C. Lindqvist

Scanning Electron Microscopy

Scanning electron microscopy (SEM) permits a three-dimensional study of the surface morphology of the organ of Corti that is very useful in evaluating the condition of the apical end of the hair cells and the stereocilia. However, some laboratories have experienced problems with curling of the basilar membrane during critical point drying of cochlear specimens prepared For SEM evaluation using the Murakami or osmium thiocarbohydrazide-procedures. This curling of the basilar membrane can obstruct the view of the reticular lamina and the ciliary ends of the hair cells.

We have used a dissection method, referred to as the anchor technique, to …

Some Thoughts About The Conservation Of Scanning Electron Microscopic Preparations Of Diatoms In A Museum Repository, J. J. Lee, C. W. Reimer, R. Mahoney

Scanning Electron Microscopy

The Scanning electron microscope (SEM) is now an indispensable tool for the study and the description of diatoms. Many new species have been described from SEM preparations and problems now arise with the preservation of designated types and other comparative material. Moisture contributes to the deterioration of diatom stubs. Special care must be taken to store stubs in vacuum desiccators in order to keep heavy metal coatings from peeling from the siliceous surfaces of diatoms. One alternative is to mount the designated type on a coverglass so that it can be inverted, mounted in Hyrax and preserved indefinitely for light …

International Law And External Threats To National Parks, Daniel Barstow Magraw

External Development Affecting the National Parks: Preserving "The Best Idea We Ever Had" (September 14-16)

41 pages.

Contains references.

Case Study: The Challenges Of The Greater Yellowstone, Bill Bryan

External Development Affecting the National Parks: Preserving "The Best Idea We Ever Had" (September 14-16)

4 pages.

Statolith Formation In Cnidaria: Effects Of Cadmium On Aurelia Statoliths, D. B. Spangenberg

Scanning Electron Microscopy

Statolith formation in Cnidaria was reviewed with an emphasis on Aurelia statoliths. The review provides information on the chemical composition, mechanisms of initiation of mineralization, and effects of environmental factors on Cnidarian statolith formation. Environmental factors discussed include modified sea water ingredients, X-irradiation, clinostat rotation, and petroleum oil ingredients. A detailed account of the effects of cadmium on mineralization and demineralization of Aurelia statoliths is given. Cadmium at dosages of 2 to 4 μM significantly reduces statolith numbers in developing ephyrae. At a dosage of 3 μM, cadmium accelerates statolith loss in unfed ephyrae studied at 4 and 8 days …

Pet Shops: The Unseen Suffering

Close Up Reports

The HSUS exposes cruelties behind closed doors

The Gill Arch Of The Striped Bass, Morone Saxatilis. Ii. Microvasculature Studied With Vascular Corrosion Casting And Scanning Electron Microscopy, Judy A. C. King, F. E. Hossler

Scanning Electron Microscopy

The gill vasculature of euryhaline striped bass, Morone saxatilis, was examined by scanning electron microscopy of corrosion casts prepared by injecting resin (either Mercox/Sevriton or L.R. White) into the ventral aorta. The vasculature of the striped bass gill appears to be similar to that of other euryhaline species. The striped bass gill has three major vascular systems: (1) a respiratory system, (2) an arterio-venous system, and (3) a nutritive system. In the respiratory system, blood from the afferent branchial artery flows to each filament via an afferent filamental artery, and from there to the highly vascularized respiratory lamellae. Lamellar …

The Effects Of Certain Oral Bacteria On The In Vitro Growth Of Bacteroides Gingivalis, Steven G. Morrow

Loma Linda University Electronic Theses, Dissertations & Projects

The effect of nine gram positive and grain negative cocci and bacilli, which are indigenous to the oral cavity, on the in vitro growth of Bacteroides gingivalis was examined in this study. A pure culture of B. gingivalis is was obtained from the American Type Culture Collection. The nine oral test organisms were Streptococcus mitis, Streptococcus mutans, Streptococcus faecalis, Staphylococcus epidermidis, Staphylococcus aureus, Corynebacteriurn hofmanii, Lactobacillus acidophilus, Branhamella catarrhalis, and Escherichia coli. The effect on the growth of B. gingivalis of the nine test organisms was evaluated on a solid medium by use of …

The Effect Of Dietary Fat Level On Tumor Development And Immunity In Balb/C Mice Fed Casein Of Wheat Gluten As Protein And Challenged With Herpes Type 2-Transformed Cells, Helen Drakou

Loma Linda University Electronic Theses, Dissertations & Projects

A high percentage of fat in the diet has been implicated through epidemiological studies in several human cancers. Studies in experimental animals have produced evidence that increasing the fat in the diet decreases the animal’s ability to restrict tumor development and growth. In previous work done in this laboratory with BALB/c mice injected with herpes simplex Type 2-transformed mouse cells (H238), the effects of a diet which greatly enhanced tumor growth was compared with one which restricted tumor growth. The latter, a low (5%) fat, low protein diet, in which the protein was casein, was also found to produce a …

Differential Effects Of Lithium Chloride On In Vitro Growth Of Clavibacter Michiganense Subsp. Nebraskense Depending Upon Inoculum Source, Mary L. Smidt, Anne K. Vidaver

Department of Plant Pathology: Faculty Publications

The bacterium Clavibacter michiganense subsp. nebraskense (Corynebacterium michiganense subsp. nebraskense) was grown in broth cultures and inoculated into corn plants. The plating efficiency of cells from broth cultures was essentially the same on nutrient broth-yeast extract and the semiselective medium for this bacterium, CNS. However, when cells were isolated from Goss bacterial wilt- and blight-infected corn, very few were recovered on CNS compared with the amount recovered on nutrient broth-yeast extract agar. When lithium chloride was omitted from the CNS, recoveries from infected corn were nearly the same as on nutrient broth-yeast extract agar. No other ingredient of CNS …

Sfa Gardens Newsletter, Sept 1986, Sfa Gardens, Stephen F. Austin State University

SFA Gardens Newsletters

No abstract provided.

The Expression Of Dna Restriction-Modification Genes Of Escherichia Coli K12, Ainu Prakash

Loma Linda University Electronic Theses, Dissertations & Projects

To facilitate the study of the expression of hsd genes in Escherichia coli K, hsdK::MudX (amp crop lac) operon fusion mutants have successfully been constructed and characterized for the first time. The results indicate that Promoters 1 and 2 for hsdK genes appear to be relatively weak, as reflected by about 50 units of beta-gal activity. Both promoters were active in all the conditions tested except during Beta-gal activity was higher in aerobic than in anaerobic growth; at 30°C and 37°C than at 42°C; and in L-broth early log phase of the culture. than in minimal media. Potential future uses …

Differential Effects Of Lithium Chloride On In Vitro Growth Of Clavibacter Michiganense Subsp. Nebraskense Depending Upon Inoculum Source, Mary L. Smidt, Anne K. Vidaver

Department of Plant Pathology: Faculty Publications

The bacterium Clavibacter michiganense subsp. nebraskense (Corynebacterium michiganense subsp. nebraskense) was grown in broth cultures and inoculated into corn plants. The plating efficiency of cells from broth cultures was essentially the same on nutrient broth-yeast extract and the semiselective medium for this bacterium, CNS. However, when cells were isolated from Goss bacterial wilt- and blight-infected corn, very few were recovered on CNS compared with the amount recovered on nutrient broth-yeast extract agar. When lithium chloride was omitted from the CNS, recoveries from infected corn were nearly the same as on nutrient broth-yeast extract agar. No other ingredient of …

The Biomineralization In Social Wasps (Vespinae): The Presence Of Statoliths, J. S. Ishay, T. Shimony, L. Arcan

Scanning Electron Microscopy

In social wasps granular aggregates occur mainly in the head region and the body integument. In the former location they are present both on the outer and inner side of the frons. On the outer side of the frons, in a groove traversing its center, there is a deep pit surrounded by a membrane which forms a cyst-like sac. In the pit of the hornet Vespa orientalis (Linne, 1771), there are aggregates comprising silicon (Sl), potassium (K), calcium (Ca) and iron (Fe), which are arranged in morula-like fashion. A granular material is secreted at the base of sensory hairs …

Permanent Noise-Induced Damage To Stereocilia: A Scanning Electron Microscopic Study Of The Lizard's Cochlea, Michael J. Mulroy

Scanning Electron Microscopy

Alligator lizards were exposed to broadband noise ranging in intensity from 106 to 132 dB SPL for two hours and permitted to recover from 19 to 62 days. Hearing loss was assessed by comparing the auditory nerve component of the cochlear potential recorded at the end of the recovery period with that recorded before the noise exposure. The stereocilia in these ears were examined with a scanning electron microscope. These sensory hairs showed pathological changes similar to those described in mammalian cochleas with noise-induced damage. In decreasing order of severity the damage included completely missing auditory papillas, missing hair cells, …

Anatomy And Morphometry Of Myocardial Capillaries Studied With Vascular Corrosion Casting And Scanning Electron Microscopy: A Method For Rat Heart, F. E. Hossler, J. E. Douglas, L. E. Douglas

Scanning Electron Microscopy

The present paper describes a procedure for preparing vascular corrosion casts of rat myocardial microvasculature. Essential components of the procedure include: partial "self clearing" of the heart in vitro; cardiac arrest by infusion of KCl; retrograde aortic root infusion of Mercox-Sevriton casting resin; KOH digestion of ventricular tissue; and desiccation and mounting of casts for scanning electron microscopy. About 50% of rats yielded complete casts. Vasculature closely paralleled muscle fiber orientation. Capillary beds characteristically exhibited branching, many intercapillary cross bridges, and occasional coil-ing. Average capillary cast diameter (5.6 μm) and intercapillary distance (15 μm) are comparable to results from in …

Topical Modes In The Preparation Of Human Spleen Specimens For Routine Scanning Electron Microscopy Studies, Haim Gamliel, Harvey M. Golomb, Eliezer Avinoah, Dorit Gurfel, Aaron Polliack

Scanning Electron Microscopy

Various preparatory techniques were used to improve scanning electron microscopy images of the fine structure of vascular, cellular, and cordal-reticular components of normal human spleens. The progressive method of fixation (GTGO) applied in the present study, allowed air drying of the tissues and rendered the specimens conductive even in newly fractured surfaces. Vascular perfusion proved necessary only in studies of the splenic blood vessels, while a simple immersion of tissue blocks in the washing solution resulted in better images of the white pulps. Interstitial (trans-splenic) perfusion was found to be superior to vascular perfusion for routine preparation of spleen tissues, …

Integrated Image And X-Ray Microanalysis Of Hepatic Lysosomes In A Patient With Idiopathic Hemosiderosis Before And After Treatment By Phlebotomy, M. I. Cleton, J. M. Roelofs, C. J. G. Blok-Van Hoek, W. C. De Bruijn

Scanning Electron Microscopy

Morphometrical and X-ray elemental information was extracted from Scanning Transmission Electron Microscopy (STEM) images of hepatic lysosomes of a patient with idiopathic hemosiderosis before and after treatment by phlebotomy. The elements of interest were (i) iron, stored in pathological quantities in hepatic lysosomal structures and (ii) cerium, used as a capture ion after a cytochemical reaction to detect acid phosphatase activity in the lysosomal structures.

Morphologically the lysosomal structures are heteromorph and the elements iron and cerium are heterogeneously distributed. With "reduced raster" (= reduced scanning area) analysis at 16 x 16 pixel points (integrating image and X-ray microanalysis), a …

Calcification In Aging Canine Aortic Valve, Kookmin M. Kim, S. H. Chang, B. F. Trump, H. Spurgeon

Scanning Electron Microscopy

Aging changes of aortic valves are thought to underlie the mechanism of calcification, which leads to calcific aortic stenosis in humans. The study of calcification in the aging valvular connective tissue has been hindered by the lack of a suitable animal model. In search of the model, canine aortic valves demonstrated age changes including calcification remarkably similar to those in humans. The mechanism of calcification was studied in the aortic valves of aged Beagles by electron microscopy. Fibroblasts in the canine aortic valves showed the most prominent age changes. The cells accumulated numerous residual bodies and appeared to disintegrate. The …