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Pancreatic Ribonuclease Functions As An Epidermal Growth Factor Receptor Ligand Independently Of Its Enzyme Activity And Contributes To Cetuximab Resistance, Heng-Huan Lee

Dissertations and Theses (Open Access)

Ribonuclease (RNase) with its catalytic enzyme activity to degrade RNAs has been shown as a diagnostic serum marker for pancreatic cancer and has also been suspected to have an unidentified cell surface receptor. Epidermal growth factor receptor (EGFR), a well-characterized receptor tyrosine kinase is an effective therapeutic target in multiple cancer types. However, clinical trials targeting EGFR have not demonstrated improved therapeutic efficacy in pancreatic cancer. Here, we show that both bovine pancreatic RNase A (bRNaseA) and human RNase 5 (hRNase5) act as EGFR ligands and directly activate EGFR to promote epithelial-mesenchymal transition. This ligand-like activity is independent of RNases’ …

The Percussionist Bodybuilder: Optimizing Performance Through Exercise And Nutrition, Anthony Joseph Merlino

UNLV Theses, Dissertations, Professional Papers, and Capstones

Do preventive therapies have the potential to help avoid common injuries among percussionists? This document investigates physiological therapy and nutrition in relation to percussion performance. My interest in this subject matter is derived from my personal experiences with injuries related to the performance of percussion. Although I have studied and played percussion professionally for more than a decade, I was unaware of certain methods of rehabilitation that were available to me prior to my injuries. As I learned about the rehabilitation process through first hand experience, I discovered that my injuries were very common among percussionists. This raised the question: …

Effects Of Breathing Cool Air During Cycling Exercise In The Heat, Christian B. Ridings

Graduate Theses and Dissertations

INTRODUCTION: The purpose of this study was to establish the ability of the Core Cooler device to prevent rises in physiological heat strain of trained male cyclists during cycling exercise in the heat. METHODS: 15 healthy male cyclists cycled at 50-70% VO2max for 75 minutes in a heat chamber of 31°C & 55% RH while breathing through the Core Cooler device under three different conditions: 1:4 ratio without ice termed control (CN), 1:4 ratio with ice termed low intermittent (LI), and at 1:1 ratio with ice termed high intermittent (HI). Data collected every 15 minutes assessing intestinal temperature (TGI), heart …

Strategic Focus On 3r Principles Reveals Major Reductions In The Use Of Animals In Pharmaceutical Toxicity Testing, Elin Törnqvist, Anita Annas, Britta Granath, Elisabeth Jalkesten, Ian Cotgreave, Mattias Öberg

Application of Alternative Methods Collection

The principles of the 3Rs, Replacement, Reduction and Refinement, are being increasingly incorporated into legislations, guidelines and practice of animal experiments in order to safeguard animal welfare. In the present study we have studied the systematic application of 3R principles to toxicological research in the pharmaceutical industry, with particular focus on achieving reductions in animal numbers used in regulatory and investigatory in vivo studies. The work also details major factors influencing these reductions including the conception of ideas, cross-departmental working and acceptance into the work process. Data from 36 reduction projects were collected retrospectively from work between 2006 and 2010. …

Can Anyone With Low Income Be Food Secure?: Mitigating Food Insecurity Among Low Income Households With Children In The Tampa Bay Area, Edgar Allan Amador

USF Tampa Graduate Theses and Dissertations

In the US over the last few years, approximately 14.5% of households experience food insecurity at some point throughout the year. While studies on food insecurity in the US have determined that household income and specifically income available to spend on food is of critical importance to food security, it is still unclear why some households with low income are able to maintain food security while others experience food insecurity in a pattern characterized as not constant but recurrent. This dissertation compares households with children at different levels of food security and insecurity using the USDA Core Food Security Module …

Cross-Bridge Mechanisms Of Skeletal Muscle Fatigue: Effects Of Hydrogen Ion, Inorganic Phosphate, And Age, Cassandra Rae Nelson

Dissertations (1934 -)

Intense muscle contraction induces high rates of glycolysis and ATP hydrolysis with resulting increases in inorganic phosphate (Pi) and H+, factors thought to induce fatigue by interfering with steps in the cross-bridge cycle. Force inhibition is less at physiological temperatures; thus the role of low pH in fatigue has been questioned. Effects of pH 6.2 and collective effects with 30 mM Pi on the pCa-force relationship were assessed in skinned fast and slow rat skeletal muscle fibers at low (15°C) and near-physiological temperatures (30°C). At Ca2+ levels characteristic of fatigue, low pH significantly depressed force at both temperatures and in …

The 24th Annual Research Conference Abstract Booklet, Nikki Lynn Rogers

University of Gondar Research Conferences

Staff members, postgraduate and senior undergraduate students of the University, invited guests and speakers participated in the conference. The annual conference of the University is meant to share experiences in research activities among juniors and seniors, staff and students, and invited guests. It is also meant to motivate students and young faculty to engage in research and also to initiate and strengthen interdisciplinary collaborations. The findings of the studies and the resulting recommendations are expected to be used in solving the diverse societal problems we have been facing.

Research activities at the University of Gondar are primarily aimed at solving …

Identification Of Microrna Biomarkers In Her2-Positive Breast Cancer, Hossam Tashkandi

Theses and Dissertations

Human epidermal growth factor receptor 2 (HER2) is overexpressed in approximately 30% of breast cancer patients with poor prognosis. In addition, microRNAs are small non-coding RNA that have been linked to many cancers. Here we investigate which miRNAs are differentially regulated by HER2 overexpression. Using quantitative reverse-transcription prolymerase chain reaction (QRT-PCR) and matching it with the clinical data acquired from Dvinge, we find five candidate miRNAs. When comparing the miRNAs’ effect on patient survival, only three miRNAs stand as good predictors of patient survival outcome. These miRNAs are miR-146a-5p, miR-181d, and miR-195-5p. When miR-146a-5p is up-regulated, which is the case …

Effect Of Whole Beetroot On Muscular Endurance And Power In Powerlifters And Physique Competitors, John H. Jewett

Theses & Dissertations

Nitrate ingestion improves aerobic exercise performance, but the efficacy in resistance training has not been established. The purpose of this study was to investigate whether consuming whole beetroot, as a means to ingest nitrate, had an ergogenic benefit in muscular power and endurance in resistance training in powerlifters and physique competitors. Thirteen men and women (mean ± SE age, 21.2 ± 0.8 y; mass, 83.1 ± 5.9 kg) that had competed in a powerlifting or a physique competition in the previous year were studied in a randomized double blind controlled crossover trial with a seven day washout period. Participants ingested …

Consequences Of Endogenous Mitochondrial Oxidative Stress In The Heart, Kristin Alisha Koenig

Dissertations, Masters Theses, Capstones, and Culminating Projects

For many years, it has been proposed that oxidative stress within the mitochondria causes mutations to the mitochondrial genome, resulting in changes in copy number per single cell. Ultimately, this compensatory increase in mtDNA, coupled with an increase in mutations, has been suggested to play a major role in the pathobiology of aging. However, evidence for this phenomenon is somewhat controversial. Importantly, oxidative stress can vary between individual cells; therefore, the overall goal of this project is to determine if oxidative stress causes both an altered copy number for mtDNA in singlecardiomyocytes and develop a methodology for sequencing mtDNA from …

Detection Methods For Small Analytes: Improvement Of Novel Spr Technology Detection Of Toxins And Toxicants, William B. Ollayos

Honors Scholar Theses

Humans are exposed to a variety of toxic substances in their day-to-day activities that may have adverse health effects if not properly identified. An integrated dual-mode instrument that combines grating-coupled surface plasmon resonance (GCSPR) and grating-coupled surface plasmon coupled emissions (GCSPCE) should allow for the efficient detection of toxic analytes in a high-content system using gold-coated superparamagnetic nanobeads. The goal of this study is to validate the binding characteristics of the reagents intrinsic to this innovative assay methodology. Competition enzyme-linked immunosorbent assays (ELISA) and surface plasmon resonance (SPR) assays were conducted on NNK toxicants and SEB toxins, which were the …

Stability Of Nano-Encapsulated Rice Bran Derived Bioactive Pentapeptide In Apple Juice, Fatima Mohammed Alessa

Graduate Theses and Dissertations

Cereal grains and their components derived Bioactive compounds such as rice bran can promote health and can be derived from Rice bran contains 12-20 % protein and could be a good source for extracting bioactive peptides. A pentapeptide with a secqunce of amino acids Glu-Gln-Arg-Pro-Arg (EQRPR) has been prepared from heat stablized defatted rice bran (HDRB) and has demonstrated anti-cancer proprerties in-vitro. This bioactive pentapeptide can thus be used as a nutraceutical by incorporating it into a suitable food system. Fruit juces can be vehicles to incorporate this pentapeptide. Fruit juices contribute to about 60% of the consumed beverages in …

Imbalance Between Neutrophil Elastase And Elafin Promotes Breast Cancer Growth And Progression, Joseph Anthony Caruso

Dissertations and Theses (Open Access)

Elafin, an endogenous serine protease inhibitor, is a critical component of the epithelial barrier against neutrophil elastase (NE) activity. The central hypothesis examined in this dissertation was that elafin has tumor suppressive properties in breast cancer. In support of this hypothesis, immunohistochemical (IHC) analysis revealed that elafin was downregulated in the majority of invasive breast tumors and a subset of pre-invasive ductal carcinoma in situ (DCIS) compared to elafin expression in the normal mammary epithelium. To understand the role of elafin in the mammary epithelium and the impetus for its downregulation during breast tumorigenesis, primary and immortalized human mammary epithelial …

Walk-Training Increases Expression Of Gdnf In Pectoralis Muscle But Not Diaphragm From Mouse, Erin Donovan

Honors Theses

Neurotrophic factors are proteins that play an important role in the development and maintenance of neurons. Recent studies have shown that neurotrophic factors may hold promise for treating damage to the nervous system caused by trauma or diseases, such as amyotrophic lateral sclerosis or Parkinson’s disease. Glial cell line-derived neurotrophic factor (GDNF) is expressed in skeletal muscles and affects peripheral motor neurons. Results of previous studies have demonstrated that exercise can increase GDNF content in skeletal muscle of rat. The goal of the current study was to examine whether expression of GDNF in skeletal muscle of mouse is also regulated …

Optimization And Standardization Of The Oxygen Radical Absorbance Capacity (Orac) Assay, Richard Low, Dr. Tory Parker

Journal of Undergraduate Research

Antioxidants, when consumed, have the ability to neutralize free radicals, suggesting their presence may protect human physiology from destructive oxidative processes1. Several assays have been developed that quantify the antioxidant capacity of compounds, and one assay that is commonly utilized by researchers and industry is the Oxygen Radical Absorbance Capacity (ORAC) assay2. Despite its popularity, significant differences in reported ORAC values occur between laboratories, leading to difficulties in comparing research. Dr. Parker and his lab conducted a series of tests to attempt to standardize and optimize the ORAC assay.

Teacher Internship Report - Anderson Union High School, Kathryn L. Teixeira

Agricultural Education: Graduate Internship Reports

This internship/project report includes validation documents required in meeting the quality criteria for secondary-level programs of instruction in agriculture. The documents are concurrently used for the Agriculture Incentive Grant review process at Anderson Union High School conducted by representatives of the California Department of Education. The internship included the practical application of occupational skills related to the horticulture industry and modification to the Greenhouse facilities at Anderson Union High School by improving ergonomics of greenhouse tables, replacing the existing siding of storage facilities and installing concrete walkways within the greenhouse. Students in the Agricultural Department were integral in all parts …

Program And Proceedings: Nebraska Academy Of Sciences 1880–2014, 134th Anniversary Year, One Hundred-Twenty-Fourth Annual Meeting, April 11, 2014

Nebraska Academy of Sciences: Programs and Proceedings

Program

Aeronautics and Space Science

Collegiate Academy: Biology

Chemistry and Physics

Collegiate Academy: Chemistry and Physics

Applied Science and Technology

Biological and Medical Sciences

Aeronautics and Space Science, Poster Session

Anthropology

Maiben Memorial Lecture: "Education, Careers and Opportunities in Engineering and Metallurgy in Nebraska," Terry Rasmussen, Nucor Steel, Norfolk, Nebraska

Teaching of Science and Mathematics

Anthropology

Earth Science

Epinephrine Regulates Aquaglyceroporin Hc-3 Expression And Subcellular Localization In Cultured Erythrocytes From The Freeze-Tolerant Treefrog, Hyla Chrysoscelis, Connor J. Ratycz

Honors Theses

Cope’s gray treefrog Hyla chrysoscelis, accumulates and distributes glycerol as a cryoprotectant in anticipation of freezing. Transmembrane glycerol and water flux in H. chysoscelis erythrocytes likely occurs through HC-3, an ortholog of mammalian aquaporin 3. HC-3 protein is in higher abundance and is preferentially localized to the plasma membrane in RBCs from cold-acclimated treefrogs as compared to warmacclimated animals. It is hypothesized that neuroendocrine agonists via receptor mediated second messenger pathways integrate signals derived from fasting, dehydration, diurnal, and/or temperature changes during cold-acclimation to regulate HC-3 expression as part of the mechanism of freeze tolerance. In this study, cultured …

The Effect Of Shear Stress, Potassium, And Adenosine On Α-1 Adrenergic Vasoconstriction Of Rat Soleus Feed Arteries, Tanner J. Heckle

Seaver College Research And Scholarly Achievement Symposium

During exercise, sympathetic nerve activity increases, augmenting the release of the neurotransmitter norepinephrine (NE) at the arterial wall and into the blood. NE binds to arterial adrenergic receptors to cause vasoconstriction, yet arteries in contracting skeletal muscle dilate during exercise. Previous evidence from Ives et al. suggests that heat and acidosis may partially inhibit constriction resulting from α-1 adrenergic receptors (termed sympatholysis). Our lab has previously demonstrated that rat soleus feed arteries respond to sympathetic signaling solely by α-1 adrenoceptors. We hypothesized that increased levels of arterial wall shear stress, potassium, or adenosine also contribute to sympatholysis, thereby reducing sympathetic …

A Study Of Biliverdin, Serum Albumin And Hemoglobin In The Scincid Lizard, Prasinohaema, Oliver Sinquefield

Honors Capstones

No abstract provided.

Redox Pioneer: Professor Joe M. Mccord, David M. Schnell, Daret K. St Clair

Graduate Center for Nutritional Sciences Faculty Publications

Dr. Joe McCord (Ph.D. 1970) is recognized here as a Redox Pioneer because he has published at least three articles on antioxidant/redox biology as first/last author that have been cited over 1000 times and has published at least 37 articles each cited over 100 times. Dr. McCord is known for the monumental discovery of the antioxidant superoxide dismutase (SOD) while a graduate student under fellow redox pioneer Irwin Fridovich and demonstrating its necessity to aerobic life. Beyond this, McCord's career is distinguished for bridging the gap from basic science to clinical relevance by showing the application of SOD and superoxide …

A Systems Biology Approach To Iron Metabolism, Reinhard C. Laubenbacher, Suzy V. Torti

UCHC Articles - Research

Iron is critical to the survival of almost all living organisms. However, inappropriately low or high levels of iron are detrimental and contribute to a wide range of diseases. Recent advances in the study of iron metabolism have revealed multiple intricate pathways that are essential to the maintenance of iron homeostasis. Further, iron regulation involves processes at several scales, ranging from the subcellular to the organismal. This complexity makes a systems biology approach crucial, with its enabling technology of computational models based on a mathematical description of regulatory systems. Systems biology may represent a new strategy for understanding imbalances in …

Consensus Report On The Future Of Animal-Free Systemic Toxicity Testing, Marcel Leist, Nina Hasiwa, Costanza Rovida, Mardas Daneshian, David Basketter, Ian Kimber, Harvey Clewell, Tilman Gocht, Alan Goldberg, Francois Busquet, Anna-Maria Rossi, Michael Schwarz, Martin Stephens, Rob Taalman, Thomas B. Knudsen, James Mckim, Georgina Harris, David Pamies, Thomas Hartung

Experimentation Collection

Since March 2013, animal use for cosmetics testing for the European market has been banned. This requires a renewed view on risk assessment in this field. However, in other fields as well, traditional animal experimentation does not always satisfy requirements in safety testing, as the need for human-relevant information is ever increasing. A general strategy for animal-free test approaches was outlined by the US National Research Council’s vision document for Toxicity Testing in the 21st Century in 2007. It is now possible to provide a more defined roadmap on how to implement this vision for the four principal areas of …

Effect Of Shear Stress Direction On Endothelial Function And Enos Phosphorylation In Soleus Feed Arteries, Blanca B. Perez, Jay Brewster, Jeffrey Jasperse

Featured Research

Blood flow feeding tissues and organs is closely regulated in order to meet metabolic and functional needs. Control of blood flow is accomplished by regulating the diameter of the arteries and arterioles feeding different organs. Several neural, hormonal, chemical and mechanical mechanisms contribute to the constriction and dilation of arteries. Shear stress, the frictional force created by streaming blood on the endothelial layer of arteries, is one of these mechanical mechanisms (1). Shear stress causes both acute and long term effects on endothelial cells (1,2,5).

Blood in arteries typically flows away from the heart towards organs (causing antegrade shear stress) …

Did Androgen-Binding Protein Paralogs Undergo Neo- And/Or Subfunctionalization As The Abp Gene Region Expanded In The Mouse Genome?, Robert C. Karn, Amanda G. Chung, Christina M. Laukaitis

Scholarship and Professional Work - LAS

The Androgen-binding protein (Abp) region of the mouse genome contains 30 Abpa genes encoding alpha subunits and 34 Abpbg genes encoding betagamma subunits, their products forming dimers composed of an alpha and a betagamma subunit. We endeavored to determine how many Abp genes are expressed as proteins in tears and saliva, and as transcripts in the exocrine glands producing them. Using standard PCR, we amplified Abp transcripts from cDNA libraries of C57BL/6 mice and found fifteen Abp gene transcripts in the lacrimal gland and five in the submandibular gland. Proteomic analyses identified proteins corresponding to eleven of the …

Determinants Of Bite Force In Four Species Of Sharks From The Gulf Of Mexico With An Emphasis On The Atlantic Sharpnose Shark, Rhizoprionodon Terraenovae, Kyle Walter Rice

Electronic Theses and Dissertations

Bite force can provide valuable information regarding the physiological ecology of an organism. However, there have been few studies wherein bite force in sharks has been considered. Herein I report on a study of the bite force of four species of sharks with particular emphasis on that of the atlantic sharpnose shark, rhizoprionodon terraenovae. Among the four species examined, blacktip shark, carcharhinus limbatus bite force was significantly higher than that of sharpnose, rhizoprionodon terraenovae. There was no significant difference between the bite force of those species compared with the same for finetooth shark, c. Isodon and spinner shark, c. Brevipinna. …

Cochlear Development And Auditory Function In The Absence Of Thyroid Hormone Transporters Mct8 And Oatp1c1, Richard Sinn

All Graduate Theses, Dissertations, and Other Capstone Projects

Thyroid hormone (TH) is essential for the development and maturation of the nervous system. The thyroid gland secretes an active form of TH, triiodothyronine, and a prohormone, thyroxine, into the blood. TH is charged, which prevents it from passively diffusing across cell membranes and thus requires cell membrane transporters to facilitate its movement into and out of cells. However, whether TH transporters are required for TH-mediated developmental events, including the auditory system, is largely unknown.

The purpose of the present study was to investigate two specific TH transporters, monocarboxylate transporter 8 (Mct8) and organic anion transporting polypeptide 1c1 (Oatp1c1), and …

Understanding Melatonin Receptor Pharmacology: Latest Insights From Mouse Models, And Their Relevance To Human Disease, Gianluca Tosini, Sharon Owino, Jean Luc Guillaume, Ralf Jockers

Neuroscience: Faculty Publications

Melatonin, the neuro-hormone synthesized during the night, has recently seen an unexpected extension of its functional implications toward type 2 diabetes development, visual functions, sleep disturbances, and depression. Transgenic mouse models were instrumental for the establishment of the link between melatonin and these major human diseases. Most of the actions of melatonin are mediated by two types of G protein-coupled receptors, named MT1 and MT2, which are expressed in many different organs and tissues. Understanding the pharmacology and function of mouse MT1 and MT2 receptors, including MT1/MT2 heteromers, will be of crucial importance to evaluate the relevance of these mouse …

The Effect Of Moderate Altitude On High Intensity Running Performance During A Game With Collegiate Female Soccer Players, Jonathan Bohner

Electronic Theses and Dissertations

Competition often requires teams that reside at sea level to compete against opponents whose residence is at a moderate altitude. This may pose a potential competitive disadvantage considering that moderate altitude may cause decrements in VO2max, distance covered, and time to exhaustion in endurance athletes. The purpose of this study was to examine the effect of altitude on game performance measures. Six NCAA Division I female soccer players (20.33 ± 1.21 y; 168 ± 6.45 cm; 62.5 ± 6.03 kg) were retrospectively examined. Comparisons were made between two competitions that were played at sea level (SL) within two weeks of …

Exploring The Relationship Between Emotional Brain Training, Stress, Depression, Food Addiction, And Weight, Bailey Regina Adams

Theses and Dissertations--Nutrition and Food Systems

Obesity is a complex issue; stress, depression, and food addiction, are several psychological conditions that can accompany an obesity diagnosis. Emotional Brain Training (EBT) was evaluated as a new approach to these conditions. Stress, depression, food addiction, and weight were assessed at baseline, after a seven week active intervention, and after a seven week no contact period. The final sample consisted of 26 obese adults. At seven week assessments, EBT participants experienced significant weight loss (p-value = 0.05) and decreased perceived stress (p-value = 0.035). Food addiction also decreased from 50% to 8.3% (or one participant). At fourteen week assessments, …