Digital Commons Network^™

Disassembly Of Actin Structures By Nanosecond Pulsed Electric Field Is A Downstream Effect Of Cell Swelling, Andrei G. Pakhomov, Shu Xiao, Olga N. Pakhomova, Iurii Semenov, Marjorie A. Kuipers, Bennett L. Ibey

Bioelectrics Publications

Disruption of the actin cytoskeleton structures was reported as one of the characteristic effects of nanosecond-duration pulsed electric field (nsPEF) in both mammalian and plant cells. We utilized CHO cells that expressed the monomeric fluorescent protein (mApple) tagged to actin to test if nsPEF modifies the cell actin directly or as a consequence of cell membrane permeabilization. A train of four 600-ns pulses at 19.2 kV/cm (2 Hz) caused immediate cell membrane poration manifested by YO-PRO-1 dye uptake, gradual cell rounding and swelling. Concurrently, bright actin features were replaced by dimmer and uniform fluorescence of diffuse actin. To block the …

The Theological And Practical Significance Of Health Reform In The Writings Of Ellen G. White, Angel Manuel Rodriguez

Journal of the Adventist Theological Society

No abstract provided.

Neuropilin In The Vascular System: Mechanistic Basis Of Angiogenesis, Hou-Fu Guo

Theses and Dissertations--Molecular and Cellular Biochemistry

The vascular system is critical for maintaining homeostasis in all vertebrates. Structural studies of Neuropilin (Nrp), an essential angiogenic receptor, have defined its role in regulating angiogenesis, the formation of new vessels from the existing vasculature. Utilizing biochemical and biophysical tools we describe the ability of Nrp to function as a co-receptor for the VEGFR receptor tyrosine kinase. Two families of Nrp-1 ligands, Vascular Endothelial Growth Factor A (VEGF-A) and Semaphorin3F (Sema3F), physically compete for binding to the Nrp-1 b1 domain, and have opposite roles. VEGF-A is a potent pro-angiogenic cytokine while Sema3F is an angiogenesis inhibitor. Using coupled structural …

Misunderstanding The Female Athlete Triad: Refuting The Ioc Consensus Statement On Relative Energy Deficiency In Sport (Red-S), Mary Jane De Souza, Nancy I. Williams, Aurelia Nattiv, Elizabeth Joy, Madhusmita Misra, Anne B. Loucks, Gordon Matheson, Marion P. Olmsted, Michelle Barrack, Rebecca J. Mallinson, Jenna C. Gibbs, Marci Goolsby, Jeanne F. Nichols, Barbara Drinkwater, Charlotte (Barney) Sanborn, Rosemary Agostini, Carol L. Otis, Mimi D. Johnson, Anne Zeni Hoch, Julia M. K. Alleyne, L. Tyler Wadsworth, Karsten Koehler, Jaci Vanheest, Paula Harvey, Amanda K. Weiss Kelly, Michael Fredericson, George A. Brooks, Emma O'Donnell, Lisa R. Callahan, Margot Putukian, Lauren Costello, Suzanne Hecht, Mitchell J. Rauh, Jacalyn Mccomb

Department of Nutrition and Health Sciences: Faculty Publications

We are concerned that readers of the IOC paper will be confused and misled by the poorly referenced statements and frank (and sometimes dangerous) errors in the paper. The IOC authors should publish a correction of these and other errors noted. Broadening research of low energy availability in other groups, such as the male athlete, athletes of diverse ethnicities and the disabled athlete may help to advance science and may one day warrant introduction of a specific term for whatever serious clinical sequelae of energy deficiency may be discovered in future research on men.

Research on the “Female Athlete Triad” …

Screening Of Mycobacterium Avium Subsp. Paratuberculosis Mutants For Attenuation In A Bovine Monocyte-Derived Macrophage Model, Elise A. Lamont, Adel M. Talaat, Paul M. Coussens, John Bannantine, Yrjo T. Grohn, Robab Katani, Ling-Ling Li, Vivek Kapur, Srinand Sreevatsan

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Vaccination remains a major tool for prevention and progression of Johne’s disease, a chronic enteritis of ruminants worldwide. Currently there is only one licensed vaccine within the United States and two vaccines licensed internationally against Johne’s disease. All licensed vaccines reduce fecal shedding of Mycobacterium avium subsp. paratuberculosis (MAP) and delay disease progression. However, there are no available vaccines that prevent disease onset. A joint effort by the Johne’s Disease Integrated Program (JDIP), a USDA-funded consortium, and USDA—APHIS/VS sought to identify transposon insertion mutant strains as vaccine candidates in part of a three phase study. The focus of the Phase …

The Effects Of Anxiety On Sensory Gating, Jonathon Meier

Honors Projects

Sensory gating is a proposed important physiological process of inhibiting neuronal responses of repetitious stimuli in the central nervous system to allocate more cognitive resources to additional salient information. Sensory gating is currently being studied to better understand psychiatric illnesses, especially those characterized by emotional changes and the inability to concentrate such as schizophrenia, ADHD, anxiety disorder, and Parkinson’s. Anxiety is a strong feeling of nervousness that occurs in all individuals at varying degrees and is associated with detrimental health effects as well as hindering concentration. Numerous brain regions are associated with anxiety levels such as the anterior limbic system, …

Simulated Diabetes Testing Experiment Using Artificially Manipulated Livestock Blood, Hollie E. Ables

Honors Theses

Due to the prevalence of diabetes and lack of simple endocrine experiments, a laboratory protocol was developed in order to increase student knowledge of the disease and the diabetic testing process. In this paper, we present the protocol for manipulating the glucose concentration of commercially available bovine blood. The bovine blood was manipulated using Ringer's solution to which glucose was added, and six samples were prepared (pre-prandial and postprandial, normal; pre-prandial and postprandial, pre-diabetic; pre-prandial and postprandial, diabetic). This laboratory exercise provides a physical, hands-on student activity that highlights pancreatic endocrinology and the results of pancreatic malfunction. The laboratory exercise …

Women In Science 2014, Clark Science Center's Summer Research Fellows Program

SURF Abstracts (Women in Science)

Women in Science 2014 summarizes research done by Smith College’s Summer Research Fellowship (SURF) Program participants. Ever since its 1967 start, SURF has been a cornerstone of Smith’s science education. In 2014, 150 students participated in SURF (141 hosted on campus and nearby eld sites), supervised by 61 faculty mentor-advisors drawn from the Clark Science Center and connected to its eighteen science, mathematics, and engineering departments and programs and associated centers and units. At summer’s end, SURF participants were asked to summarize their research experiences for this publication.

The Extent Of Heat On Health And Sustainable Farming In Ghana - Bawku East, Kwasi Frimpong, Jacques Oosthuizen, Eddie J. Van Etten

Research outputs 2014 to 2021

Little is known about the health effects of heat in outdoor work and appropriate work and rest schedules for farmers working in developing countries. As temperatures continue to increase in tropical regions, such as Northern Ghana, it is necessary to evaluate how farmers experience and respond to high heat exposures. In this study, WBGT (Wet Bulb Globe Temperature) estimates and the ISO work / rest standards were applied to a cohort of farmers in the rural areas of Bawku East, Northern Ghana, to assess how farmers respond to high heat and how much they rest to protect their health, as …

Genetic Sex Conditions And Redefining Sex, Jayce O'Shields

Student Scholarship

Western culture has a tendency to value binaries and discreet categories that separate its social structure and provide a sense of order and organization. The value placed on binaries and categories may be advantageous in some aspects, but when it starts to infringe upon the legal and medical rights of individuals not easily placed in either binary category, it can become less advantageous.

A baby is usually classified as either male or female shortly after birth, and all future legal, social, and economic actions and rights of that individual are more or less decided according to this classification. A problem …

Reading And Listening To Music Increase Resting Energy Expenditure During Indirect Calorimetry In Healthy Adults , Blaire Snell

Theses and Dissertations

The Academy of Nutrition and Dietetics has developed an evidence analysis library (EAL) for Nutrition and Dietetics professionals. The EAL is updated by members through workgroups consisting of experts in their fields, most often in response to unanswered questions. One such question is: what kinds of activities can be done during the rest period of an indirect calorimetry test in a healthy population? The objective of our study was to determine if listening to self-selected relaxing music or reading on an electronic device or a magazine effects resting energy expenditure (REE) as measured by an indirect calorimetry test in a …

Growth Pattern, Allison Kudlah

SWITCH

"Growth Pattern" is an installation that merges botanical tissue culture with ornamental design. In this installation, tobacco leaves are die-cut into patterns and cultured in petri dishes, where new leaf growth develops over time.

The Envenoming Pandemic And The Misguided Views Of Snakes: Developing Sustainable Relationships Between Native Poisonous Snakes Of India And The Citizens Of Maharashtra Through The Use Of The Irula Model., Destiny Villanueva

Animal Science

Due to its vast population and general negative consensus regarding snakes, India is notorious for having more snakebite incidents than any other country. Snakes are simultaneously revered and feared in Indian religion and culture. This stigma is perpetuated through propaganda, family beliefs, and innate fears of snakes.These stigmas are more pronounced within rural areas, such as the region of Mahad within the state of Maharshtra. Envenoming is endemic in these areas, and others like them. The World Health Organization identified envenoming as one of the most neglected tropical diseases of the 21st century (Nature India, 2013). Additionally, both nonvenomous and …

Development Of Isotags For Nmr Based Metabolite Profiling And Applications, Fariba Tayyari

Open Access Dissertations

NMR spectroscopy is a powerful analytical tool for both qualitative and quantitative metabolite profiling analysis. However, accurate quantitative analysis of biological systems especially using one dimensional NMR has been challenging due to signal overlap. In contrast, the enhanced resolution and sensitivity offered by chemoselective isotope tags have enabled new and enhanced methods for detecting hundreds of quantifiable metabolites in biofluids using NMR spectroscopy or mass spectrometry. In this thesis we show improved sensitivity and resolution of NMR experiments imparted by 15N and 13C isotope tagging which enables the accurate analysis of plasma metabolites. To date, isotope tagging has been used …

University Of Nebraska At Omaha Biomechanics Research Building Newsletter, Fall 2013, Biomechanics Research Building

Biomechanics Annual Report

This issue features:

Updates - Featured Story: Our New Home, American Society of Biomechanics Meting, Nonlinear Workshop 2013, European Nonlinear Workshop, and Staff Updates;

Projects - VA Peripheral Arterial Disease, Motor Development, Optical Topography System, Effects of Virtual Reality, Virtual Reality, Robotic Surgery, Sports Medicine, Amputee, Proof of Concept Grant, Lateral Stepping, The Marriage of Music and Math, Chronic Obstructive Pulmonary Disease, Balance, NASA Grant, Back Pain Research, and Faculty Research International Grants.

Other Content - Other Visitors/Tours, Student Awards, Travels, and Campaign for Nebraska.

The Adrenal Cortex Cell Line H295r Releases Interleukin-6, Timothy E. Lindsay, Dr. Allan M. Judd

Journal of Undergraduate Research

Up to this point, the study of adrenal glands has utilized mostly rat and bovine models. However, in the research of human physiology, human models are preferable to animal models. But human adrenal glands are difficult to obtain, and when they are obtained, experiments often have limited sample sizes. In addition, human adrenal glands available for research are often functionally altered by disease. Also, donors with healthy adrenal glands may be administered multiple pharmacological agents which modify adrenal functions. These obstacles show how a model that provides an unlimited quantity of tissue with consistent secretion patterns would take precedent over …

Gait Variablility Is Altered In Older Adults When Listening To Auditory Stimuli With Differing Temporal Structures, Jeffrey P. Kaipust, Denise Mcgrath, Mukul Mukherjee, Nikolaos Stergiou

Journal Articles

Gait variability in the context of a deterministic dynamical system may be quantified using nonlinear time series analyses that characterize the complexity of the system. Pathological gait exhibits altered gait variability. It can be either too periodic and predictable, or too random and disordered, as it is the case with aging. While gait therapies often focus on restoration of linear measures such as gait speed or stride length, we propose that the goal of gait therapy should be to restore optimal gait variability, which exhibits chaotic fluctuations and is the balance between predictability and complexity. In this context, our purpose …

Bioengineering The Expression Of Active Recombinant Human Cathepsin G, Enteropeptidase, Neutrophil Elastase, And C-Reactive Protein In Yeast, Eliot T. Smith

Electronic Theses and Dissertations

The yeasts Pichia pastoris and Kluyveromyces lactis were used to express several recombinant human proteins for further biochemical characterization. Two substitution variants of recombinant human enteropeptidase light chain (rhEPL) were engineered to modify the extended substrate specificity of this serine protease. Both were secreted as active enzymes in excess of 1.7 mg/L in P. pastoris fermentation broth. The substitution variant rhEPL R96Q showed significantly reduced specificities for the preferred substrate sequences DDDDK and DDDDR; however, the rhEPL Y174R variant displayed improved specificities for these substrate sequences relative to all other reported variants of this enzyme. The neutrophil serine proteases human …

Effects Of High-Velocity Versus Low-Velocity Resistance Training On Resting Metabolic Rate And Functional Performance In Older Adults, Laura Ashleigh Morgan

Graduate Theses and Dissertations

The purpose of this study was to compare the effects of a 12-week high-velocity resistance training (HVRT) protocol to a traditional low-velocity resistance training (LVRT) protocol on resting metabolic rate (RMR) and other selected measures of muscular and functional fitness in older adults. Nineteen adults between the ages of 65 and 82 participated: 8 HVRT, 7 LVRT, and 4 controls (CTRL). Initially, no differences existed between groups except for age (p = .016). HVRT (75.6 years) was older than LVRT (69.6 years) and CTRL (69.3 years). The exercise intervention consisted of 2 days/week sessions for 12 weeks at 3 sets …

Effect Of Shear Stress On Ecnos Expression And Dilation In Soleus Feed Arteries, Blanca Perez, Jay L. Brewster, Jeffrey Jasperse

Featured Research

Shear stress causes artery dilation and increased expression of endothelial cell nitric oxide synthase (ecNOS) in coronary and placental arteries. We sought to determine the importance of shear stress in maintaining normal dilation and normal levels of ecNOS in rat soleus feed arteries (SFA). SFA were isolated from male Sprague-Dawley rats and cannulated for in vitro microscopy (Fig. 6). SFA were exposed to no shear stress, low shear stress, or high shear stress conditions for 4 hours. After 4 hours, endothelium-dependent dilation (acetylcholine: ACh) and endothelium-independent dilation (sodium nitroprusside: SNP) were tested. Arteries were then uncannulated, mRNA was isolated, and …

The Effect Of Shear Stress, Potassium, And Adenosine On Α-1 Adrenergic Vasoconstriction Of Rat Soleus Feed Arteries, Tanner J. Heckle, Jeffrey Jasperse

Featured Research

During exercise, blood flow increases to the working skeletal muscle primarily because of dilation of the arteries and arterioles feeding the muscle. Sympathetic nerve activity also increases during exercise, augmenting the release of the neurotransmitter norepinephrine (NE) at the arterial wall and into the blood. NE acts to constrict blood vessels; however, arteries and arterioles within contracting skeletal muscle dilate despite the increased NE present. This has led to the concept of functional sympatholysis (4), the idea that a chemical released from contracting skeletal muscle interferes with NE signaling. NE acts by binding to adrenergic (alpha and beta) receptors, and …

Cycling Time Trial Performance Is Not Enhanced By Either Whey Protein Or L-Alanine Intake During Prolonged Exercise, Adam B. Schroer

Masters Theses, 2010-2019

Previous studies have reported that adding protein (PRO) to a carbohydrate (CHO) solution can enhance endurance performance. This ergogenic effect may be a function of additional calories from supplemental protein/amino acids, but this thesis has not been directly examined. Additionally, L-alanine (ALA) is readily oxidized when provided during exercise; the impact that this has on metabolism and prolonged endurance performance is unknown. The purpose of this investigation was to assess performance and various cardiovascular and metabolic outcomes during prolonged cycling, to independently gauge the efficacy of whey PRO hydrolysate and ALA supplementation. Eight trained male and female cyclists (age: 22.3±2.0 …

The Effect Of Skeletal Muscle Mass On Basal Metabolic Rate In College-Age Males 2013, Chase Vaughn

Master's Theses

There are many misconceptions within the fitness community. One such misconception is that an increase in muscle mass will have a large increase in basal metabolic rate, yet this may not be the best way for successful weight loss. The purpose of this study was to determine the strength of the correlation between skeletal muscle mass and basal metabolic rate. The hypothesis tested was that basal metabolic rate is strongly correlated with skeletal muscle mass. The participants were college-aged males (n =17). Skeletal muscle mass (SMM) was estimated using the skin-fold circumference model (SFCM): SMM (kg) = Ht (0.00744 CAG2 …

Putative Glucosyltransferase 11 From Citrus Paradisi: Cloning, Recombinant Expression In Yeast, And Substrate Screening., Bruce E. Williams

Undergraduate Honors Theses

Plant secondary products, which include the flavonoids, have a variety of roles in plant systems. Their roles include UV protection, antifeedant activity, pollinator attraction, stress response, and many others. Flavonoids also have effects on human physiology. Glucosylation is an important modification of many flavonoids and other plant secondary products. In grapefruit, glucosylation is important in the synthesis of the bitter compound naringin. Glucosyltransferases catalyze glucosylation reactions. Putative plant secondary product glucosyltransferases may be identified by the loosely conserved “PSPG box” amino acid sequence, and eleven have been isolated to date in Citrus paradisi. With current knowledge, however, biochemical characterization …

Manipulating Aktivated Metabolism Via Mtorc1, Ivan Von Hack Prestinary

HIM 1990-2015

Although poorly understood, normal cells and cancerous cells of the same type exhibit different patterns of nutrient consumption, processing and utility of metabolic substrates. Differences in substrate uptake, preference, and alternately emphasized metabolic pathways offer opportunities for selective targeting of cancer versus stroma. This may be accomplished by using a sequential approach of nutrient deprivation and pharmaceutical perturbation of metabolic pathways to inhibit cellular proliferation. The purpose of this study was to investigate the effects of restricting glucose and glutamine concentrations, in vitro, to levels that resemble a potential human fasting state. The mammalian target of rapamycin (mTOR), a mediator …

Early Complexity Supports Development Of Motor Behaviors In The First Months Of Life, Stacey C. Dusing, Leroy R. Thacker, Nikolaos Stergiou, James C. Galloway

Journal Articles

Complexity in motor behavior is a hallmark of healthy systems. The purpose of this study was to investigate postural complexity during development of early motor behaviors and under two conditions. Twenty-two infants participated from 1 to 6 months of age. Linear and nonlinear measures of displacement of the center of pressure at the base of support were used to quantify magnitude and temporal structure of postural control. Behavioral coding was used to quantify the emergence of midline head control and early reaching. Results suggest that infants have complexity in postural control strategies early in development. This complexity decreases as infants …

Phylometagenomics: A New Framework For Uncovering Microbial Community Diversity, Christopher J. Friedline

Theses and Dissertations

Microbial communities are recognized as major drivers of global biogeochemical processes. However, the genetic diversity and composition, as well as processes leading to the origin and diversification of these communities in space and time, are poorly understood. Character- ization of microbial communities using high-throughput sequencing of 16S tags shows that Operational Taxonomic Unit (OTU) abundances can be approximated by a gamma distribu- tion, which suggests structuring around small numbers of highly abundant OTUs and a large proportion of rare OTUs. The current methods used to characterize how communities are structured rely on multivariate statistics, which operate on pair-wise distance matrices. …

Characterization Of Human Gut Microbiota Dynamics Using Model Communities In Gnotobiotic Mice, Nathaniel Patrick Mcnulty

All Theses and Dissertations (ETDs)

The human gut is colonized by a diverse array of microbes, collectively referred to as the microbiota. The microbiota's complexity poses significant challenges in characterizing the rules dictating its assembly, inferring the functional roles of its component species, and understanding how communities sense and respond to changes in their habitat. We developed defined, representative model communities comprised of sequenced human gut bacteria that could be characterized in a highly controlled manner in gnotobiotic mice, plus a suite of scalable molecular tools for assaying community properties. These tools were first used to evaluate how the microbiota is impacted by probiotic bacterial …

Camk-Ii: An Integral Protein In Cell Migration, Jamie Josephine Avila Mcleod

Theses and Dissertations

Coordinated inductive and morphogenetic processes of gastrulation establish the zebrafish body plan. Gastrulation includes massive cell rearrangements to generate the three germ layers and shape the embryonic body. Three modes of cell migration must occur during vertebrate gastrulation and include: epiboly, internalization of the presumptive mesendoderm and convergent extension (C&E). C&E movements narrow the germ layers mediolaterally (convergence) and elongate them anteroposteriorly (extension) to define the embryonic axis. The molecular mechanisms regulating coordinated cell migrations remain poorly understand and studying these has become of great interest to researchers. Understanding cell migration during development is highly relevant to a number of …

The Effects Of Selected Drugs On The Memory Recall Of Sleep Deprived Mice, Jenn Pauldurai

Capstone Research Projects

Humans often make use of the neurotransmission altering effects of drugs such as caffeine, ephedrine, and ethanol. These drugs induce changes in memory and learning ability, specifically when used for sleep deprivation. English white mice (Mus musculus) have been shown to exhibit similar cognitive changes when administered drugs while sleep deprived. A sample of 20 mice was divided into three experimental groups (each group receiving a different drug) and one control group. Maze-testing demonstrated that mice performance decreased with sleep deprivation (Treatment-1) but all groups returned to baseline performance when injected (IP) with selected drugs (Treatment-2), regardless of …