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Changes In Frontoparietotemporal Connectivity Following Do-As-I-Do Imitation Training In Chimpanzees (Pan Troglodytes), Sarah M. Pope, Jared P. Taglialatela, Sara A. Skiba, William D. Hopkins

Faculty Articles

Human imitation is supported by an underlying ‘mirror system’ principally composed of inferior frontal (IF), inferior parietal (IP), and superior temporal (ST) cortical regions. Across primate species, differences in fronto-parieto-temporal connectivity have been hypothesized to explain phylogenetic variation in imitative abilities. However, if and to what extent these regions are involved in imitation in non-human primates is unknown. We hypothesized that ‘Do As I Do’ (DAID) imitation training would enhance white matter integrity within and between fronto-parieto temporal regions. To this end, four captive chimpanzees (Pan troglodytes) were trained to reproduce 23 demonstrated actions, while four age/sex-matched controls were trained …

Analysis For Science Librarians Of The 2017 Nobel Prize In Physiology Or Medicine: The Life And Work Of Jeffrey C. Hall, Michael Rosbash, And Michael W. Young, Neyda V. Gilman

Library Scholarship

Jeffrey C. Hall, Michael Rosbash, and Michael W. Young are the 2017 Nobel Prize in Physiology or Medicine Laureates, having earned the award for their “discoveries of molecular mechanisms controlling the circadian rhythm.” They identified the genes involved in the circadian rhythm mechanism and explained how all of the different pieces of the mechanism work together. These discoveries explain how the biological cycles of Earth’s organisms correspond to the rotation of the planet, acting as an inner clock. Their research also provides further opportunities to continue learning about the role of circadian rhythm and its relationship to human health (Nobelprize.org …

Brief, High-Intensity Interval Exercise Improves Selective Attention In University Students, Jeremy J. Walsh, Charlotte Dunlap, Jonathan Miranda, David B. Thorp, Derek S. Kimmerly, Michael Tschakovsky, B J. Gurd

International Journal of Exercise Science

International Journal of Exercise Science 11(5): 152-167, 2018. This study aimed to investigate the effect of very brief, high-intensity interval exercise (HIIE) on selective attention in university students. As a follow-up, we investigated whether HIIE performed prior to a university lecture improves retention of lecture material. A single-group counterbalanced post-test only design was used for this study. 22 university students (19 females; age = 20.0 ±1.0 years) performed a HIIE and control visit on separate days. During the HIIE session, participants performed 4 separate body-weight exercises for 1 set each, consisting of eight 20 s intervals interspersed with 10 s …

Katalyst Pilot Study: Using Interactive Activities In Anatomy And Physiology To Teach Children The Scientific Foundation Of Healthy Lifestyles, Rebecca L. Hagedorn, Kathryn Baker, Sara E. Dejarnett, Tyler Hendricks, Melissa Mcgowan, Lauren Joseph, Melissa D. Olfert

Faculty & Staff Scholarship

This pilot study evaluated the impact of the Katalyst curriculum, a fifth-grade experiential learning program, on students’ knowledge of a healthy lifestyle’s impact on body functions. Katalyst’s interactive curriculum spans two days and includes four, 60-min stations on body systems: cardiovascular/endocrine, gastrointestinal, neurological, and respiratory/musculoskeletal. Three schools were recruited, and two schools completed the intervention sessions. Prior to beginning the stations, fifth-grade students completed a 37-item questionnaire to assess knowledge and perceptions. Students completed the same survey at the end of the Katalyst intervention. Teachers at the school also completed a survey post intervention to provide feedback on the program. …

Investigating The Effects Of Antibiotics Perturbation On Lactobacillus Species Metabolomics Profiles, Sophia Lee, Kundi Yang, Jiangjiang (Chris) Zhu

Summer Research

Our gut microbiota contains a vast array of cells that influence human physiology, metabolism, nutrition and immune function. Lactobacillus is a group of Gram-positive, lactic acid bacteria that is naturally present in the human gastrointestinal tract. Some species are probiotics that provide beneficial health effects to humans. Many studies have investigated and noted their various health benefits to their human host, including prevention of certain diseases such as colon cancer, breaking down of food, and absorption of nutrients. Maintaining the balance of our normal flora is essential - even small perturbations to the microbiota can have significant health effects. It …

The Investigation Of Lactoferrin Nitration: Quantification, Function, And Inhibition, Amani Yahya Alhalwani

Electronic Theses and Dissertations

Lactoferrin (LF) is an iron-binding glycoprotein of molecular mass ca. 80 kDa that is predominantly found in mammalian body fluids. Lactoferrin is a multifunctional protein that has a wide range of properties such as antibacterial, antiviral, anti-inflammatory, and anti-allergic functions. Tyrosine residues in the protein play a part in many lactoferrin functions. Protein tyrosine nitration modification represents an oxidative and nitrosative stress process which can be caused by the exposure of proteins to oxidants from air pollution or disease. Understanding the way nitrated lactoferrin interacts with the biochemical environment of the body is thus important to the broader goal of …

Control Of Salmonella Virulence By Microbiota-Derived And Dietary Fatty Acids, Zhenrun J. Zhang

Student Theses and Dissertations

The intestinal microbiota plays critical roles in human physiology and diseases. While recent research has revealed many mechanisms by which gut microbiota influences host immunity to defend against invading pathogens, how microbiota directly antagonizes pathogen virulence is less studied. In particular, gut microbiota produces large amounts and varieties of small molecules that may impact both host immunity and pathogen virulence. In this thesis, I describe how fatty acids, derived from both gut microbiota and diet, contribute to attenuation of virulence of enteric pathogen Salmonella. In Chapter 1, I review how dietary and microbiota metabolites affect different aspects of host-microbe interactions. …

Simultaneous Use Of Physiological Sensors For A Neuromarketing Task, Charles Descheneaux

Electronic Theses and Dissertations

Physiological measurements have become more popular in Psychological research over the past ten years. These advancements allowed different objective sensors to become another measurement tool in a scientific arsenal of collecting data. Traditionally, performance and after task subjective measures have been used for most studies in Psychological research. With the opportunity to use these subjective measures along with objective measures, more data can be collected during research and therefore potentially produce better quality conclusions. Eye Tracking (ET), functional near infrared (fNIR), transcranial Doppler ultrasound (TCD), electrocardiogram (EKG) and the electroencephalogram (EEG) have shown great promise in their ability to produce …

Differentiating Human Populations Based On K-Mer Classification Of Hand Bacteria, Thrisha Doppala

Graduate Theses, Dissertations, and Problem Reports (ETD)

Bacterial communities found in and on the human body are not only used in studying human health conditions but are also effective in differentiating individuals due to their distinct profiles. Human palm regions harbor relatively more diverse bacterial communities and are indicative of population groups, life styles, geographic locations, age groups and health conditions. Sequences extracted from hypervariable region V3 of the 16S rRNA bacterial gene of hand bacterial samples from 9 different population groups were classified into Operational Taxonomic Units (OTU) with GreenGenes reference taxonomy using RDP (Ribosomal Database Project) classifier. Frequencies of identified OTUs were used to study …

Psychophysiology Meets Computer Science: Predicting The Magnitude Of Participant Physiological Response With Machine Learning, Avonie Parchment

Electronic Theses and Dissertations

The present inquiry uses methods from psychophysiology and machine learning to reduce overall error in classification models. The field of psychophysiology, though rooted in decades of experimentation, has never reached the same level of precision as some aspects of medical inquiry. In fact, while some medical regression models, when determining some way to classify a patient's illness based on certain symptoms, can result in highly significant results with large effect sizes, equal levels are virtually unheard of in psychophysiology. The present investigation attempts to unravel some part of this mystery and determines some possible reasons for the difficulty in finding …

The Effects Of Exercise Training On Cardiovascular-Related Circulating Micrornas, Jacob Luther Barber

Theses and Dissertations

PURPOSE: MicroRNAs (miRNAs) are small regulatory RNAs that post transcriptionally modify mRNAs and control gene expression. Circulating miRNAs are significantly altered following a single session of exercise, however the effects of exercise training on the circulating miRNA profile is unclear. Therefore, the purpose of the present study was to determine the effects of endurance exercise training on the abundance of targeted circulating miRNAs and the association of changes in miRNA levels with concomitant changes in cardiometabolic traits, in a subsample of adults from the HERITAGE Family Study.

METHODS: This exploratory analysis examined 20 previously sedentary adults from the HERITAGE Family …

Acute Cardiovascular Responses To Novel Combinations Of Resistance And Aerobic Exercise, Travis Wakeham

Dissertations, Master's Theses and Master's Reports

Cardiovascular disease (CVD) is the leading cause of death globally. One of the most effective forms of prevention and treatment is physical activity. However, recent studies have suggested that resistance exercise can increase arterial stiffness. Maintaining adequate strength is critical for performing activities of daily living, occupational-related tasks, and sport movements. The purpose of this project was to determine if novel combinations of resistance and aerobic exercise could offer musculoskeletal benefits without adverse cardiovascular consequences. In Study 1, we examined the effect of order (i.e. aerobic before resistance, or resistance before aerobic) on arterial stiffness when combining these exercise …

Milk Exosomes Are Bioavailable And Distinct Microrna Cargos Have Unique Tissue Distribution Patterns, Sonia Manca, Bijaya Upadhyaya, Ezra Mutai, Amy T. Desaulniers, Rebecca A. Cederberg, Brett R. White, Janos Zempleni

Department of Nutrition and Health Sciences: Faculty Publications

Exosomes participate in cell-to-cell communication, facilitated by the transfer of RNAs, proteins and lipids from donor to recipient cells. Exosomes and their RNA cargos do not exclusively originate from endogenous synthesis but may also be obtained from dietary sources such as the inter-species transfer of exosomes and RNAs in bovine milk to humans. Here, we assessed the bioavailability and distribution of exosomes and their microRNA cargos from bovine, porcine and murine milk within and across species boundaries. Milk exosomes labeled with fluorophores or fluorescent fusion proteins accumulated in liver, spleen and brain following suckling, oral gavage and intravenous administration in …

Physiological Responses In Reindeer To The Application Of A Conducted Electrical Weapon, Camilla L. Lieske, Kimberlee B. Beckmen, Larry L. Lewis

Human–Wildlife Interactions

Conducted Electrical Weapons (CEWs) have potential as effective alternatives to chemical restraint for short-term non-routine capture and handling as well as aversion hazing of wildlife. To assess immediate and delayed physiologic effects of exposure to a CEW, we assigned 15 captive reindeer (Rangifer tarandus tarandus) to one of three treatment groups: immobilized with carfentanil and xylazine (CX), 10 second exposure to a CEW, or exposure to the CEW while immobilized with CX (CEW+CX). Blood samples were collected pre-treatment, immediately post-intervention, 10 min, 20 min, 4 hours, and 24 hours post-intervention. Physiologic effects were evaluated by analysis of blood, …

Ample Evidence For Fish Sentience And Pain, Lynne U. Sneddon, David C.C. Wolfenden, Matthew C. Leach, Ana M. Valentim, Peter J. Steenbergen, Nabila Bardine, Donald M. Broom, Culum Brown

Animal Sentience

The majority of commentaries are supportive of our position on the scepticism that muddies the waters surrounding fish pain and sentience. There is substantial empirical evidence for pain in fish. Animals’ experience of pain cannot be compared to artificial intelligence (AI) because AI can only mimic responses to nociceptive input on the basis of human observations and programming. Accepting that fish are sentient would not be detrimental to the industries reliant on fish. A more proactive discussion between scientists and stakeholders is needed to improve fish welfare for the benefit of all.

Characterization Of Neural Cells Derived From Reelin-Deficient Schizophrenic Patient Ips Cells, Nicole Roberts

Electronic Theses and Dissertations

Reelin is a large, extracellular glycoprotein that binds to several membrane receptors on neural stem cells (HNSCs), neural progenitor cells (NPCs), and neuroblasts of mammals to direct their migration. Previously, our lab established the presence of Reelin increased migration of wild-type fetal-derived HNSC's, both in vitro and in vivo. In addition, we demonstrated that Reelin protein treatment also increases the formation of radial glia via Notch-1 signaling, in vitro. Radial glia are precursors to NPCs, as well as a scaffold for neuroblast migration during cortical lamination. Reelin has long been associated with Schizophrenia (SZ). Because post-mortem brains are limited to …

The Protective Effects Of Chrysin And Flunixin Meglumine Against Excess Copper In Male Rats, Pinar Tatli Seven, Burcu Gül Baykalir, İsmai̇l Seven, Tuba Parlak Ak, Neşe Başak Türkmen, Hakan Güllüoğlu

Turkish Journal of Veterinary & Animal Sciences

Thirty-six Sprague Dawley male rats were housed in individual cages and randomly divided into six groups: control, copper sulfate (Cu; 500 ppm body weight (BW)/day), flunixin meglumine (FM; 2.2 ppm BW/day), chrysin (chrysin; 50 ppm BW/day), copper sulfate + FM (Cu + FM; 500 ppm BW/day of copper sulfate and 2.2 ppm BW/day of FM), and copper sulfate + chrysin (Cu + Chrysin; 500 ppm BW/day of copper sulfate and 50 ppm BW/day of chrysin). Feed intake (FI) in the Cu + Chrysin group significantly increased in comparison with that of the Cu group (P < 0.01). Cu excess significantly increased malondialdehyde, indicating oxidative stress. Chrysin and FM administration significantly decreased malondialdehyde levels and increased the superoxide dismutase and catalase activities in the liver and kidney tissues (P < 0.001). Serum TNF-α levels were significantly lower in the Cu + FM and Cu + Chrysin groups in comparison to the Cu group (P < 0.001). It was seen that FM and chrysin treatments alleviated degeneration, necrosis, and apoptosis in the liver and kidney tissues of the Cu-treated rats. Chrysin appeared to ameliorate the adverse effects on FI and liver and kidney tissues by scavenging where the free radicals are located and increasing the activity of antioxidants.

Fluid Flow In A Krogh Cylinder: A Model For A Single Capillary And Surrounding Tissue, Xianjie Qiu

Masters Theses

"A model describing the flow of plasma from the capillaries to the interstitial space in the extravascular tissue and back into the capillaries is constructed and solved. The flow through the tissue or the interstitial spaces is described by the Brinkman equation for porous medium, modified to account for the presence of cells considered as spheres. The flow of blood through the capillaries is considered to be that of a Newtonian fluid. The flow between capillary and tissue is coupled by the permeability of the capillary wall. The nature of flow and its magnitude was found to be highly dependent …

A Review Of Heat Stress Policies In The Context Of Climate Change And Its Impacts On Outdoor Workers: Evidence From Zimbabwe, Bigboy Ngwenya, Jacques Oosthuizen, Martyn Cross, Kwasi Frimpong, Cynthia N. Chaibva

Research outputs 2014 to 2021

Record-breaking summer heat events are increasing in frequency in Zimbabwe and 2016 was a particularly hot year with the country experiencing its worst heat wave event in decades. Currently, Zimbabwe has no coordinated public health response to deal with heat wave events and no specific data on heat-related morbidity and mortality. The country has no legislation for protecting workers against environmental heat exposure, particularly those most vulnerable who are employed in the informal sector. These workers are also at risk due to their outdoor work environments. The article outlines the state of climate and heat stresses in Zimbabwe, as benchmarked …

Xenotransplantation:The Science, The Advantages, The Ethics, Chana Erlbaum

The Science Journal of the Lander College of Arts and Sciences

Xenotransplantation is a breakthrough medicinal technology that is an attempt to change the lives of millions of people. The problems in the current organ transplant system risk the lives of patients each and every day. Intense waiting periods and extremely costly procedures exemplify the stress and pressure that these patients face as an attempt to save their own lives. Xenotransplantation is the idea of growing human organs in a different species, using incredible stem-cell and CRISPR technology. This can introduce an answer to some of the issues in the current transplant system. Many technical and ethical issues are becoming relevant …

Determination Of The Effects Of Different Maillard Reaction Products On The Taxonomic Composition Of The Gut Microbiota, Nesreen Hamdan Aljahdali

Graduate Theses and Dissertations

The Maillard Reaction (MR) is a non-enzymatic chemical reaction which results in linkage between the amino group of amino acids and the carbonyl group of reduced sugars. This reaction generates Maillard reaction products (MRPs) which are not present naturally in foods, and are responsible for a range of colors, odors, flavors, and other sensory properties. Conflicting reports of MRPs impacts on human health are probably due to the fact that bioconversion of these digestible molecules by the gut microbiota has been marginally taken into account. This study aimed to determine the effects of different MRPs on rodent’s gut microbiota through16S …

Piglet Gut Microbiota Responses To Exposure To Outdoor Simulated Environment And Formula Feeding, Nguyen Vo

Graduate Theses and Dissertations

Several lines of evidence suggest that early-life gut microbiota composition is a crucial determinant of the susceptibility to diseases later in life. Understanding the evolution of early-life gut microbiota and critical factors that can modify this microbial community will espouse the prevention or reversion of health risk originating from dysbiosis of the gut microbiota in the early stage. In this research, we hypothesized that alterations in the environment (simulation of rural conditions by soil exposure) and feeding mode (breastmilk versus formula milk) will have significant impacts on the establishment of the gut microbiota in early life. At first, in literature …

The Body Mass Index Of San Francisco Cold Water Swimmers - Comparisons To U.S. National And Local Populations, And Pool Swimmers, Brendan T. Crow, Ellicott C. Matthay, Stephen P. Schatz, Mark D. Debeliso, Thomas J. Nuckton

International Journal of Exercise Science

International Journal of Exercise Science 10(8): 1250-1262, 2017. To determine if cold-water swimmers have substantial differences in BMI, which might have a protective effect against heat loss during swims in cold water without wetsuits, and to determine if obesity is more or less prevalent in cold-water swimmers, we compared the body mass index (BMI) values of 103 recreational open-water swimmers (mean age 54.3 ±10.8 years) to data from various population groups. Swimmers swam consistently throughout the winter months, in the San Francisco Bay (water temperature range: 9.6° C [49.3 ° F] to 12.6° C [54.7 ° F]), without wetsuits. After …

Mitochondrial Reactive Oxygen Species In Lipotoxic Hearts Induces Post-Translational Modifications Of Akap121, Drp1 And Opa1 That Promote Mitochondrial Fission, Kensuke Tsushima, Heiko Bugger, Adam R. Wende, Jamie Soto, Gregory A. Jenson, Austin R. Tor, Rose Mcglauflin, Helena C. Kenny, Yuan Zhang, Rhonda Souvenir, Xiao X. Hu, Crystal L. Sloan, Renata O. Pereira, Vitor A. Lira, Kenneth W. Spitzer, Terry L. Sharp, Kooresh I. Shoghi, Genevieve C. Sparagna, Eva A. Rog-Zielinska, Peter Kohl, Oleh Khalimonchuk, Jean E. Schaffer, E. Dale Abel

Department of Biochemistry: Faculty Publications

Rationale: Cardiac lipotoxicity, characterized by increased uptake, oxidation and accumulation of lipid intermediates, contributes to cardiac dysfunction in obesity and diabetes. However, mechanisms linking lipid overload and mitochondrial dysfunction are incompletely understood.

Objective: To elucidate the mechanisms for mitochondrial adaptations to lipid overload in postnatal hearts in vivo.

Methods and Results: Using a transgenic mouse model of cardiac lipotoxicity overexpressing long-chain acyl-CoA synthetase 1 in cardiomyocytes, we show that modestly increased myocardial fatty acid uptake leads to mitochondrial structural remodeling with significant reduction in minimum diameter. This is associated with increased palmitoyl-carnitine oxidation and increased reactive oxygen species (ROS) generation …

Ligand Modulation Of Sidechain Dynamics In A Wild-Type Human Gpcr, Lindsay D. Clark, Igor Dikiy, Karen Chapman, Karin Ej Rodstrom, James Aramini, Michael V. Levine, George Khelashvili, Soren Gf Rasmussen, Kevin H. Gardner, Daniel M. Rosenbaum

Publications and Research

GPCRs regulate all aspects of human physiology, and biophysical studies have deepened our understanding of GPCR conformational regulation by different ligands. Yet there is no experimental evidence for how sidechain dynamics control allosteric transitions between GPCR conformations. To address this deficit, we generated samples of a wild-type GPCR (A2AR) that are deuterated apart from 1H/13C NMR probes at isoleucine d1 methyl groups, which facilitated 1H/13C methyl TROSY NMR measurements with opposing ligands. Our data indicate that low [Na+] is required to allow large agonist-induced structural changes in A2AR, and that patterns of sidechain dynamics substantially differ between agonist (NECA) and …

Body Size Predicts Cardiac And Vascular Resistance Effects On Men's And Women's Blood Pressure, Joyce M. Evans, Siqi Wang, Christopher Greb, Vladimir Kostas, Charles F. Knapp, Qingguang Zhang, Eric S. Roemmele, Michael B. Stenger, David C. Randall

Biomedical Engineering Faculty Publications

Key Points Summary

• We report how blood pressure, cardiac output and vascular resistance are related to height, weight, body surface area (BSA), and body mass index (BMI) in healthy young adults at supine rest and standing.
• Much inter-subject variability in young adult's blood pressure, currently attributed to health status, may actually result from inter-individual body size differences.
• Each cardiovascular variable is linearly related to height, weight and/or BSA (more than to BMI).
• When supine, cardiac output is positively related, while vascular resistance is negatively related, to body size. Upon standing, the change in vascular resistance is positively related to size. …

Characterization Of Filifactor Alocis And Its Immune Evasion Strategies Employed Against Human Neutrophils., Cortney Linn Armstrong

Electronic Theses and Dissertations

Periodontal disease is among the most common of inflammatory conditions and is caused by bacterial and host derived factors. The presence of bacteria drives the recruitment of neutrophils, professional phagocytes, to migrate to specific oral sites where they produce potent antimicrobials to kill their target. However, this inflammation and production of antimicrobials must be strictly regulated to minimize collateral host tissue damage. Human neutrophils recognized the oral pathogen Filifactor alocis through Toll-like receptor (TLR) 2 and upon binding, activated both p38 MAPK and ERK signaling pathways, known to be involved in neutrophil cell migration and degranulation. F. alocis also stimulated …

Metabolic Regulation By Stress Mediators In Fetal Ovine Skeletal Muscle And Adult Bovine Myoblasts, Elena Marie Merrick

Department of Animal Science: Dissertations, Theses, and Student Research

Our objective was to determine the effect of stress mediators such as inflammatory cytokines and β adrenergic agonists on developing skeletal muscle. Fetal sheep exposed to chronic maternal inflammation had decreased skeletal muscle glucose oxidation rates when stimulated with insulin or TNFα. In addition, activation of Akt by insulin was decreased after maternal inflammation when compared to controls and there were not differences in skeletal muscle fiber type ratios between the two treatment groups. This indicates that maternal inflammation does not affect overall oxidative capacity in the developing fetus, but rather insulin signaling pathways are disrupted.

We sought to determine …

The Effect Of Blinded Hydration State On Thermoregulation And Performance In Male Cyclists, Jon David Adams

Graduate Theses and Dissertations

Purpose: The purpose of these studies was to observe the effect of dehydration on exercise performance while subjects were blinded to their hydration status. Methods: Study 1: Seven male cyclists (weight: 71±8 kg, body fat: 14±6%, VO2peak: 59.4±6 ml∙kg-1·min-1) exercised for 2 hours on a cycle ergometer at 55% VO2peak, in a hot-dry environment (35°C, 30% rh), with a nasogastric (NG) tube under euhydrated (EUH-NT) and hypohydrated (DEH-NT) conditions. In both trials, thirst was matched by drinking 25 mL every 5 min. In the EUH-NT trial sweat losses were fully replaced via the NG tube. Following the 2 hours of …

A Numerical Study Of The Interaction Between One Dimensional Carbyne Chain And Single Stranded Dna, Zeina Salman

Graduate Theses and Dissertations

resolution at the single nucleotide level when developing DNA sequencers. The purpose of this research was to numerically study the electrical properties associated with the interaction between 1D carbon chain, known as carbyne, and ssDNA. First, the electrical properties of the carbyne chain were calculated. Second, the electrical properties of the carbyne chain were calculated in the presence of different ssDNA bases. Analyzing the differences between the two cases led to determining the effects of these different bases on the electrical properties. The numerical simulation approach conducted in this research was based on the first-principle simulation. The first-principle simulation was …