Digital Commons Network^™

Costs Of Group Locomotion: How Infant-Carrying And Group Members Mediate Walking Speed Decisions In North American And East African Populations, Leah M. Bouterse

Honors Projects

A major portion of humans’ activity-based energy expenditure is taken up by locomotion, particularly walking. Humans can offset the energetic expenditure of walking in numerous ways, both evolutionary (such as changes in body shape) and culturally. Behaviorally, people can choose to walk in a variety of ways, including alone or with a group, carrying loads, and walking quickly or more slowly. All of these behaviors have energetic outcomes and as such can be important windows into how populations and groups adjust to different constraints. While sex differences in speed of paired walkers have been established by others, the dynamics of …

Weather And Children’S Physical Activity; How And Why Do Relationships Vary Between Countries?, Flo Harrison, Anna Goodman, Ester M.F. Van Sluijs, Lars Bo Carson, Greet Cardon, Rachel Davey, Kathleen F. Janz, Susi Kriemler, Lynn Molly, Angie S. Page, Russell R. Pate, Jardena J. Puder, Luis B. Sardinha, Anna Timperio, Niels Wedderkopp, Andy P. Jones

Faculty Publications

Background

Globally most children do not engage in enough physical activity. Day length and weather conditions have been identified as determinants of physical activity, although how they may be overcome as barriers is not clear. We aim to examine if and how relationships between children’s physical activity and weather and day length vary between countries and identify settings in which children were better able to maintain activity levels given the weather conditions they experienced.

Methods

In this repeated measures study, we used data from 23,451 participants in the International Children’s Accelerometry Database (ICAD). Daily accelerometer-measured physical activity (counts per minute; …

Post- Dinner Satiety With The Paleolithic Diet Compared To Usual Diet, Michael Jones

Honors Projects

The Paleolithic (Paleo) Diet mimics the diet consumed by Paleolithic Humans. This diet is low in carbohydrate and high in healthy fats. Because of this, it has been found to be highly satiating. We theorized that consumption of the Paleo diet would result in higher satiety ratings as compared to a usual diet. Men and Women with metabolic syndrome (n=4) consumed their usual diet at both baseline and the wash out period and the Paleo diet with high and sedentary exercise. Satiety was measured with a validation tool twice with usual diet and once with the paleo diet with sedentary …

Transcranial Direct Current Stimulation And Sports Performance, Dylan J. Edwards, Mar Cortes, Susan Wortman-Jutt, David Putrino, Marom Bikson, Gary Thickbroom, Alvaro Pascual-Leone

Publications and Research

No abstract provided.

The Chemistry Of Matching Italian Foods With Wines, Samuel Collinsworth, Rachael Maynard

Senior Theses

This report attempts to explain the science behind matching Italian food with wines. Common knowledge states that certain wines make better pairings with certain food dishes, but the general public knows little about why these foods and wines make better matches. This paper aims to use examples of tastings of Italian foods with different wines in order to educate its readers on the science behind the pairings. Before these tastings are analyzed, background information is provided about the science of taste. A description of the five basic tastes is given, separating them into appetitive and aversive tastes, and the chemistry …

Hibernator And Non-Hibernator Responses To Acute Changes In Water Intake, Sydni S. Andruskiewicz

All College Thesis Program, 2016-2019

Hibernating animals undergo dramatic changes in metabolic rates during torpor. One of most notable changes in these animals is the ability to maintain blood pressure and perfuse certain organs. Consequentially, blood perfusion to the kidneys is greatly decreased and the ability to concentrate urine halts. However, about once a week, torpor is interrupted and the animal becomes active to rewarm itself about once a week. This activity induces rapid regeneration of the extracellular osmotic gradient of the kidney, and allows urine to be concentrated. Nonetheless, regaining the extracellular osmotic gradient creates a potentially fatal consequence to the kidney cells. To …

The Effect Of Ambient Temperature On Recovery Of Surgical Stress In Sprague-Dawley Rats, Romie D. Powell

Masters Theses, 2010-2019

Laboratory animals are housed at ambient temperatures ranging from 20°C – 26°C per recommended guidelines. Rats in particular typically prefer ambient temperatures (Tamb) of ~27°C (Brown et al, 2011). When rats undergo surgical instrumentation for experimental use, they often recover at normal room temperature (~21°C). While this is comfortable to those maintaining them, it may lead to a cold thermal stress for the rats. It is hypothesized that housing rats at ambient temperatures away from their preferred Tamb could lead to a thermal stress, which adversely affects surgical recovery. To address this, rats (220g-350g) were surgically instrumented with a radiotelemetry …

Validity Of Daytime Urinary Frequency As A Tool For Assessing Hydration, Monica Ziebart

Health, Human Performance and Recreation Undergraduate Honors Theses

Introduction: It is known that 24-h void number is a valid way to assess hydration status. However, no studies have examined if urinary frequency just during the waking hours (~18 h) is a valid indicator of hydration status. Purpose: This study was conducted in order to determine if void number during only the waking hours was a valid indicator of hydration status compared to total voids over a 24-hour period. Methods: The subjects in this study consisted of 14 males and 18 females 18-80 yrs old who were healthy and not currently taking any body fluid altering medications. This study …

Answers To Common Misconceptions About Biological Evolution, Leah M. Abebe, Blake Bartels, Kaitlyn M. Caron, Adam M. Gleeson, Samuel N. Johnson, Tyler J. Kluza, Nicholas W. Knopik, Kristen N. Kramer, Masiel S. Maza, Kaitlyn A. Stava, Kaitlyn Sullivan, Jordan T. Trimble, Robert M. Zink

Papers in Evolution

“When you have an established scientific emergent truth, it is true whether or not you believe in it.” Neil deGrasse Tyson, Science in America. Very few people outside of a particular scientific discipline can actually say they understand it, because most do not have the training to “speak the language.” They are then not particularly bothered by its tenets and predictions. Of all the major branches of science, however, evolutionary biology is an exception to this generalization because even though people are not versed in the field, they sometimes have a negative, knee-jerk objection. This objection is often because they …

Undergraduate Student Perceptions And Awareness Of Genetic Counseling, Amanda Gerard

Dissertations and Theses (Open Access)

Genetic counseling (GC) is a rapidly expanding field, and the supply of certified genetic counselors is currently unable to keep up with job demand. Research is fairly limited regarding the awareness and perceptions potential genetic counseling program applicants have of the field and what factors most influence their interest in the field. This study aimed to examine some of these topics. The current study includes data collected from 1,389 undergraduate students in the sciences at 23 universities across the United States who were surveyed regarding information related to their awareness, perceptions, knowledge, and interest in GC. Data analysis was performed …

Expression Of Mammalian Dna In A Non-Native Cell System, Taylor Hyatt

Undergraduate Honors Thesis Projects

In this study, we aimed to investigate the interaction between glucocorticoid receptors and calcium channels. To begin our research, we began to optimize the transfection efficiency of a glucocorticoid receptor within human embryonic kidney cells. Results found that this non-native cell was able to be transfected with pK7-GR-GFP DNA. Although it was unexpected, we were unable to obtain a transfection efficiency of 10% to move forward with other planned experiments. However, through our efforts, we were able to achieve a transfection efficiency of 0.04% and troubleshoot many difficulties that prevented obtaining a higher transfection efficiency. It was found that the …

Creation Of An Immunodeficient Hla-Transgenic Mouse (Humamice) And Functional Validation Of Human Immunity After Transfer Of Hla-Matched Human Cells, Yang Zeng, Bingrun Liu, Marie-Therese Rubio, Xinyue Wang, David M. Ojcius, Ruoping Tang, Antoine Durrbach, Zhitao Ru, Yusen Zhou, Yu-Chun Lone

All Dugoni School of Dentistry Faculty Articles

Research on human immunology has been hindered by the lack of optimal small animal models, given that the protective immune responses of human and non-human species show significant differences. However, due to ethical constraints[1] and the high cost of clinical trials, it is urgent to improve the current animal models that can mimic faithfully human physiology, particularly the human immune system (HIS). HIS mice had been generated recently by engrafting human hematopoietic stem cells (hHSCs) or human peripheral mononuclear cells (hPBMCs) into highly immuno-deficient mice such as NSG, NOG or NRG mice. However, a major experimental drawback for studies using …

Pravastatin™ Suppresses Venous Shear Stress Dependent Induction Of Aquaporin 1 Protein Expression In Human Umbilical Vein Endothelial Cells In Vitro, Raphael J. Crum

Honors Theses

Background: Aquaporins (AQPs) are transmembrane water channels that facilitate osmotically-driven transmembrane water flux. AQP1 is abundantly expressed in vascular endothelium, where it functions in endothelial cell migration, wound healing, and cell volume regulation in response to mechanical stimuli. AQP1 protein abundance is enhanced in cultured human umbilical vein endothelial cells (HUVECs) in response to fluid shear stress. Statins, a pharmacological class of HMG-CoA reductase inhibitors, suppress the early development of vein graft intimal hyperplasia by improving endothelial cell function, most likely by affecting endothelial response to arterial shear stress. Statins may also differentially regulate the expression of specific AQPs by …

Lactose Intolerance: An Overview Of The Facts And Their Implications, Noelle M. Yeo

Honors Program: Senior Projects (Public)

Lactose intolerance is often blamed for the symptoms such as bloating, diarrhea, gas, abdominal pain, and nausea, that ail many people. Patients often do not seek proper diagnosis from a physician and create their own treatment plans, severely restricting lactose intake, without professional guidance. Even those who do seek the care of a physician find that diagnosis is complicated by less-than ideal testing and confusion due to the symptoms common to many other conditions. The misconceptions and inability to confirm a diagnosis of lactose intolerance can cause nutrient deficiencies in these patients, as well as begin a pattern of unnecessary …

Human Stress Protein Response To Exercise And The Environment, Rebecca Cuthbert

UNO Student Research and Creative Activity Fair

Stress proteins are molecular chaperones that protect cells from the damaging effects that can be caused by exercise and environmental changes. Cold-inducible RNA binding protein (CIRP) and RNA binding motif protein 3 (RBM3) protect cells from mild hypothermia and hypoxia. They allow cells to rapidly respond to environmental stresses by regulating gene expression. Heat shock proteins 27, 70, and 90 protect skeletal tissue from exercise and heat stress. They accelerate cellular repair and prevent misfolding of proteins. These proteins, especially CIRP and RBM3, have been studied in cell cultures and animal models, but limited data exist in humans. Purpose: To …

Superhero Physiology: The Case For Captain America, Stanley P. Brown, Johneric W. Smith, Matthew Mcallister, Leeann Joe

Publications

Using pop icons in the science classroom represents a creative way to engage often-distracted students in a relevant and, perhaps more importantly, fun way. When the pop icon is as universally known as Captain America, the pedagogical stage is set. However, when the movies can also be employed to link dramatic references to the science concepts at hand, we may have a very powerful tool by which linkages between fiction and science can be forged. In this regard, Captain America’s performances in several movies to date can be used to explain actual science. Granted, script writers and movie directors may …

Ecological Correlations Of Dietary Food Intake And Mental Health Disorders, Jordan Hoerr, Joshua Fogel, Benjamin Van Voorhees

Publications and Research

This paper examines the ecological association of dietary food intake with mental health outcomes on the group level across countries. Published data from the World Mental Health Survey were used to compare lifetime prevalence of four categories of mental health disorders (anxiety disorders, mood disorders, impulse control disorders, and substance use disorders) with a country’s fish/seafood and sugar/sweetener supply quantity using the Spearman rank correlation. Data were compared for 17 countries across the world. Sugar and sweetener supply quantity was significantly and positively associated with anxiety disorders (rho = 0.75, p = 0.001), mood disorders (rho = 0.75, p = …

Understanding The Association Between Nutrition And Depression By Assessing Diet Quality, Omega-3, Cholesterol, And Sleep Quality In College Students, Kandice H. Abramson

Theses and Dissertations

Background: Depression is one of the most common health problems in college students. Understanding the associations between diet quality, total blood cholesterol, sleep quality, and depressive symptoms could aide in improving the mental health of this population.

Objective: The purpose of this study was to evaluate the association between diet quality, omega-3 fatty acid intake, total blood cholesterol levels, sleep quality, and depressive symptoms in college students.

Design: Data were collected from a convenience sampling of participants in a general physical fitness course at Illinois State University. The Healthy Eating Index 2010 (HEI), a composite measure to assess adherence to …

The Molecular Basis For The Lack Of Inflammatory Responses In Mouse Embryonic Stem Cells And Their Differentiated Cells, William D'Angelo, Chandan Gurung, Dhiraj Acharya, Natalya Ortolano, Fengwei Bai, Yan-Lin Guo

Faculty Publications

We reported previously that mouse embryonic stem cells do not have a functional IFN-based antiviral mechanism. The current study extends our investigation to the inflammatory response in mouse embryonic stem cells and mouse embryonic stem cell–differentiated cells. We demonstrate that LPS, TNF-α, and viral infection, all of which induce robust inflammatory responses in naturally differentiated cells, failed to activate NF-κB, the key transcription factor that mediates inflammatory responses, and were unable to induce the expression of inflammatory genes in mouse embryonic stem cells. Similar results were obtained in human embryonic stem cells. In addition to the inactive state of NF-κB, …

Bio Buzz Newsletter, Issue 8.1, November 2014, Department Of Biology

Biology Buzz Newsletter

Denison University, Department of Biology newsletter for Fall 2014.

Bio Buzz Newsletter, Issue 6, December 2012, Department Of Biology

Biology Buzz Newsletter

Denison University, Department of Biology newsletter for Fall 2012.

Bio Buzz Newsletter, Issue 5.2, May 2012, Department Of Biology

Biology Buzz Newsletter

Denison University, Department of Biology newsletter for Spring 2012.

Bio Buzz Newsletter, Issue 5, December 2011, Department Of Biology

Biology Buzz Newsletter

Denison University, Department of Biology newsletter for Fall 2011.

Diabetes Mellitus: Concerns, Treatment Options, And The Future, Ryan Curry

Biology: Student Scholarship & Creative Works

Diabetes is a serious disease that is becoming very serious worldwide. This review will discuss some of the treatment options that are available for type two diabetes with a comparison to insulin management. It will discuss the history, genetics, economics, and also the future of diabetes management.

A New Model For Phd Elective Course, H R. Ahmad, Satwat Hashmi, M H. Mahmood

Department of Biological & Biomedical Sciences

No abstract provided.

Low Cost, Multi-Purpose Genotyping Panels For Dairy And Beef Cattle, Michelle Judge

Theses

rhe selection of animals based on DNA has revolutionised animal breeding, but the associated high cost of obtaining genotypes has limited its uptake. The objective of this thesis was to develop a low-cost, low-density, multi-purpose genotyping panel for the procurement of reliable genotype information, and to quantify the long-term consequences of using such low-density genotype panels in breeding programs. The objectives were achieved through a combination of real-life cattle genotype data and simulations to mimic a cattle population. The in-silico development of genotype panels was based on actual genotypes from up to 58,705 beef cattle. Alternative novel strategies were used …

Nitrosative Stress Sensing In Porphyromonas Gingivalis: Structure And Function Of The Heme Binding Transcriptional Regulator Hcpr, Benjamin R. Belvin

Theses and Dissertations

Porphyromonas gingivalis, a Gram negative anaerobe implicated in the progression of periodontal disease, is capable of surviving and causing infection despite high levels of reactive nitrogen species found in the oral cavity due to its efficient nitrosative stress response. HcpR is an important sensor-regulator that plays a vital step in the initiation of the nitrosative stress response in many Gram negative anaerobic bacteria. We employ a combination of X-ray crystallography, SAXS, resonance Raman spectroscopy, UV-Vis spectroscopy, and molecular biology techniques to better understand this key regulator. Knockout of the hcpR gene in W83 P. gingivalis results in the inability of …

The Role Of N-Myc Downstream Regulated Gene 1 In Breast Cancer Lipid Metabolism, Christopher James Sevinsky

Legacy Theses & Dissertations (2009 - 2024)

Altered carbohydrate and lipid metabolism are increasingly well characterized hallmarks of aggressive breast cancers. While aerobic glycolysis, or “the Warburg effect”, is a well-established metabolic adaptation exploited by tumor cells, the understanding of unique aspects of cancer lipid metabolism lags behind. This is especially true regarding the coordination of complex lipid synthesis and trafficking pathways, which remains poorly understood. N-Myc Downstream Regulated Gene1 (NDRG1) is overexpressed in many solid tumors, but its function is unclear. The importance of NDRG1 is best exemplified by the effect of null mutations on human physiology: inactivating mutations give rise to the severe autosomal recessive …

Planaria: An Animal Model That Integrates Development, Regeneration And Pharmacology, Oné R. Pagán

Biology Faculty Publications

Although planarians are established model organisms in developmental biology and regeneration studies, in the last forty years or so, they have caught the attention of pharmacologists, especially to study the pharmacology of drugs of abuse. This review covers the following topics: some fundamentals of the history of animal models and planarians in biomedical research; an abbreviated story of systematic pharmacology research using planarians as a model organism; an example of how planarians are contributing to the search for compounds against acute cocaine toxicity; an analysis of the number of papers on planarians and pharmacological topics from 1900- 2016; some perspectives …

Altitude- And Sex-Specific Variation In Roosting Ecology And Thermoregulation Of Myotis Lucifugus In Yellowstone National Park, Alexandra C. Slusher

Theses and Dissertations--Forestry and Natural Resources

Fifty-nine female and six male little brown myotis (Myotis lucifugus) were radio-tagged during the summers of 2012, 2013, 2014, and 2015 in Yellowstone National Park. The grand models for daily maximum skin temperature (F_98,154 = 1.55, P = 0.007), daily minimum skin temperature (F_98,154 = 1.33, P = 0.05), and daily variation in skin temperature (F_98,154 = 1.56, P = 0.006) were significant across roost type and reproductive condition class for adult females. Roosts were classified into Types A (warmest roosts), B (roosts with largest daily temperature variance), and C (stable and cool roosts) depending …