Digital Commons Network^™

Invasive African Fig Flies (Zaprionus Indianus) Have Reduced Cold Acclimation Ability Compared To Cosmopolitan Drosophilid Species, Mia Hollekim, Lucy Smith, Priscilla A. Erickson

Biology Faculty Publications

Zaprionus indianus, an invasive species of fruit fly originating from Africa, has successfully inhabited eastern North America but is thought to be seasonally limited by cold temperatures. We compared the cold acclimation abilities of Z. indianus to six drosophilid species. Each species’ survival at -5°C was tested following acclimation periods at 10°C, 15°C, and 25°C. We found that Z. indianus had a similar baseline cold tolerance to other cosmopolitan species and acclimated following treatment at 15°C. Overall, cold acclimation in Z. indianus was better than tropical species but weaker than some cosmopolitan species, suggesting cold may restrict Z. indianus …

Student Worksheets Based On The Local Wisdom Of Besilek Serawai For Sixth-Grade Elementary Science Learning, Mice Agustin, Tomi Hidayat, Irwandi Irwandi

Jurnal Pendidikan Sains

This study aims to develop a Student Worksheet (Lembar Kerja Peserta Didik [LKPD]) based on the local wisdom of Besilek Serawai for sixth-grade elementary science education and to evaluate its validity. The study employed the ADDIE development model, consisting of five stages: Analyze, Design, Develop, Implement, and Evaluate. The developed LKPD integrates Besilek Serawai, a traditional martial art of the Seluma community, with the concept of the human locomotor system in science instruction. The worksheet was validated by two subject-matter experts and two media experts. Material validation yielded an average score of 4.26, equivalent to a validity percentage of 85.2%, …

The Effect Of Project-Based Learning Model On Entrepreneurship (Pjbl-E) With A Deep Learning Approach On Students' Problem-Solving And Cognitive Skills, Ricce Oktasari, Irwandi Irwandi, Siti Darwa Suryani

Jurnal Pendidikan Sains

This study aims to examine the effect of the Entrepreneurship-based Project Based Learning (PjBL-E) model integrated with a pedagogical Deep Learning approach on students’ problem-solving skills and cognitive learning outcomes in biology learning. The novelty of this study lies in the integration of entrepreneurship values and pedagogical deep learning principles into Project Based Learning to create contextual and meaningful learning experiences. Entrepreneurship integration encourages students to develop innovative products, identify real-world problems, and apply biological concepts in practical and socio-economic contexts. Meanwhile, the pedagogical Deep Learning approach promotes reflective thinking, conceptual understanding, critical analysis, and the ability to connect theory …

Zinc Biofortification Technologies , Edible Safety , And Physiological Function Regulation In Edible Mushrooms, Wu Bingbing, Shi Nengpai, Su Jianwei, Hou Xuemei, Lin Xiangyang

Food and Machinery

Zinc (Zn) is an essential trace element for humans, and dietary zinc deficiency is an important global public health issue. Edible mushrooms have become highly promising carriers for zinc biofortification due to their natural mineral accumulation ability and the capacity to convert inorganic zinc into organically bound states with higher bioavailability. The enrichment and absorption of zinc, intracellular homeostasis regulation, and subcellular distribution patterns during the cultivation process of edible mushrooms were summarized, and the key factors influencing the efficiency of zinc biofortification, including fungal species, zinc sources (inorganic, organic, or nano forms ),application methods,and cultivation conditions, were evaluated. In …

Advances In Extraction And Detection Technologies For Veterinary Drug Residues In Food, Luo Qiaoqiao, Yuan Yufeng, Jia Yunzhu, Chen Qingya, Tan Fang, Zhao Xin

Food and Machinery

Veterinary drug residues constitute a global challenge that poses significant threats to food safety and public health. This review systematically summarizes recent advances in detection technologies for veterinary drug residues in food, with a particular focus on the performance characteristics and application status of sample extraction techniques (including liquid-liquid extraction, solid phase extraction, accelerated solvent extraction, QuEChERS, and magnetic solid phase microextraction ),as well as analytical methods (including chromatographic-mass spectrometric techniques, immunoassays, capillary electrophoresis, biosensors, and quantum dots -based approaches ). Comparative analysis reveals that accelerated solvent extraction (ASE ) and QuEChERS have emerged as mainstream pretreatment techniques owing to …

Children With Cerebellar Malformations And Co-Occurring Autism: A Phenomenological Study Exploring Challenges Faced With The Dual Diagnosis Process, Service Acquisition, And Psychosocial Impacts, Ladeana W. Gambill

Doctoral Dissertations and Projects

Parents of children who have been diagnosed with cerebellar malformations, such as Dandy-Walker Malformation, often face confusion and frustration as they are given uncertain prognoses about their child’s future. The uncertainty is further compounded when there are concerns for autism spectrum disorder (ASD), and testing is sought, with conflicting answers on whether their child has autism or not. Acquisition of autism-specialty services may be delayed if a diagnosis of autism is not received in a timely manner. The purpose of this interpretive phenomenological study was to understand the lived experiences and challenges faced by parents of children with cerebellar malformations …

Diversifying Clinical Trial Participation: Leveraging Digital Health Innovations And Community Outreach For Recruitment And Retention Improvements, Blake Aaron Lambert

Doctoral Dissertations and Projects

The purpose of this qualitative study was to explore how digital tools and community outreach can improve recruitment and retention in clinical trials, particularly among underserved populations. This study examined key barriers to participation, the potential benefits and limitations of digital tools, and strategies to engage underrepresented groups. Surveys were conducted with healthcare professionals (HCPs) and community members to gather perspectives on participation in clinical research. NVivo was used to code and analyze data. The analysis identified themes related to cultural barriers and trust, awareness, logistical barriers and accessibility, structural barriers and relevance, digital tools, and engagement. Participants were expected …

Preoperative Low-Intensity Blood Flow Restriction Training Does Not Improve Quadriceps Strength Or Hypertrophy After Anterior Cruciate Ligament Reconstruction, Daniel Mcgurren, Kaycee Glattke, Molly Klanderman, Jedediah Lee, Anikar Chhabra, Arthur De Luigi

Journal for Sports Neuroscience

Objectives: To investigate the efficacy of a preoperative low-intensity blood flow restriction (LI-BFR) training intervention for patients undergoing anterior cruciate ligament reconstruction (ACLR).

Design: Pilot study

Methods: Twenty-eight patients undergoing primary ACLR participated in either a LI-BFR training or sham intervention at a rate of 2 sessions per week for 2 weeks before surgery. Quadriceps strength and cross-sectional area were measured via handheld dynamometry and leg press test before and after the intervention before surgery and at 5 different time points after surgery, through 24 weeks after surgery.

Results: Adjusting for sex, time, and strength/hypertrophy at baseline, there were no …

Influence Of Smoking On Mlh1 Gene Polymorphism: Insights Into Colorectal Cancer Incidence, Ali Mohammed Al-Abedi, Rand Muhammed Abdul-Hussein Al-Husseini

Al-Bahir

Background: Colorectal cancer (CRC) is a leading cause of cancer-related mortality worldwide. The MutL Homolog 1 (MLH1) gene plays a critical role in DNA mismatch repair (MMR). Evidence suggests cigarette smoking increases CRC risk, potentially interacting with genetic factors.

Materials and Methods: This case-control study involved 50 Iraqi CRC patients and 50 age- and gender-matched healthy controls. The PCR-RFLP technique was employed for genotyping the -93G/A (rs1800734) polymorphism. Statistical analysis included Chi-square, Fisher's Exact Test, and logistic regression adjusted for confounders. Hardy-Weinberg Equilibrium (HWE) was assessed in controls.

Results: The frequency of the rs1800734 variant was significantly higher in CRC …

Dual-Action Antibacterial Agents Targeting Biofilm Reduction And Membrane Disruption In Staphylococcus Aureus, Rahmat Folashade Zakariyah, Waliyullahi Akorede Husain, Elohor Precious Samuel, Wonder Olanipekun Oloruntoba, Fatmah Opeyemi Saka, Khalid Ayinde Oyelami, Mariam Temitope Baker

Al-Bahir

Antimicrobial resistance in Staphylococcus aureus is a serious global health problem as the organism rapidly develops resistance against widely used antibiotics. This study sought to investigate the antibacterial and antibiofilm properties of γ-Tocopherol, a natural compound from Ocimum gratissimum, as well as its synergism with 5-hydroxymethylfurfural (5-HMF) against S. aureus. The antibacterial activities and minimum inhibitory concentrations of the compounds were determined using agar diffusion and microbroth dilution methods, respectively. Biofilm reduction was evaluated using 2,3,5-triphenyltetrazolium chloride (TTC) assay and membrane integrity was evaluated using the crystal violet microtiter plate assay and the ion leakage assay. The results …

Evaluating The Efficacy Of Green-Method-Prepared Zinc Oxide Nanoparticles On Gene Expression Of Efflux Pumps In Klebsiella Pneumoniae Bacteria Isolated From Women With Gestational Diabetes, Wissam Qadry Mutaab, Rana J. Shaker

Al-Bahir

Vaginitis is one of the most common women's health problems, and its severity increases in pregnant women with gestational diabetes due to higher susceptibility to infection and increased rates of antibiotic resistance. The research problem: The increasing prevalence of multidrug-resistant isolates of Klebsiella pneumoniae and its association with AcrAB-TolC ejaculation pumps limits treatment efficacy, while the effect of green-method nanoparticles on gene expression of these pumps remains unclear. This paper aimed to detect the acrA and acrB genes in K. pneumoniae isolates and evaluate the effect of zinc oxide nanoparticles prepared with garlic and rosemary extracts on their gene expression. …

A Rho5-Cnc1-Dnm1 Signalling Module Coordinates Multi-Organelle Execution Of Regulated Cell Death In Yeast, Justin Bauer

Theses and Dissertations

Regulated cell death is a conserved process with major implications for health and disease, yet how yeast cells integrate stress signals to determine survival versus death remains unclear. This thesis investigates Cyclin C (Cnc1), a conserved Mediator kinase module component with both transcriptional and mitochondrial roles. Together with Cdk8 Cnc1 primarily represses stress response genes, while upon stress it translocates to the mitochondria to induce mitochondrial fragmentation with the dynamin-related GTPase Dnm1. Using structure-guided separation-of-function mutants, I demonstrate that Cnc1-dependent death requires its mitochondrial function rather than its transcriptional role, and that its effector Dnm1 acts as a signaling node …

Cardiovascular Disease Subtypes And Alzheimer's Disease: Phenotypic And Genetic Associations In The Uk Biobank And All Of Us Research Program, Aili Toyli, Chen Zhao, Kuan Jui Su, Hui Shen, Hong Wen Deng, Qing Hui Chen, Qiuying Sha, Weihua Zhou

Michigan Tech Publications

BACKGROUND: Cardiovascular disease (CVD) and Alzheimer's disease (AD) are major public health concerns that share overlapping risk factors and potential mechanistic pathways. Although vascular contributions to cognitive decline are well documented, the specific relationships between AD and different CVD subtypes remain poorly understood. METHODS: In this cross-sectional study, we examined associations between AD and 11 CVD subtypes using logistic regression models in 2 large biobanks: the UK Biobank (n=502 133) and the All of Us Research Program (n=287 011). Models were adjusted for demographic, lifestyle, and clinical covariates. We also explored genetic overlap between AD and CVD traits through proximity-based …

Antioxidant Potential Of A Non-Heme Iron Complex: Mitigating H2O2-Induced Cellular Damage, Magy A. Mekhail, David M. Freire, Cameron Bowers, Grant Elam, Nora Del Bosque, Hannah K. Pyle, Sarah K. Dunn, Spencer Lanyon, Timothy J. Hubin, Giridhar Akkaraju, Kayla N. Green

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Iron complexes with antioxidant activity have garnered significant interest for both general and medical applications, yet few studies have yielded functional nonheme iron-based mimics for H₂O₂ mitigation in aqueous environments. Here, we investigate water-soluble nonheme iron complexes derived from 12-membered pyridinophanes (^CF₃PyN₃, PyN₃, ^NMe₂PyN₃, and Py₂N₂) to evaluate the impact of ligand scaffolds on H₂O₂ decomposition activity both in vitro and in a cellular model. Speciation and kinetic analyses reveal that both electronic and geometric modulation of the macrocyclic …

The Helminthic Status Of Selected Tanzanian Subterranean Rodents, The Root-Rats (Spalacidae: Tachyoryctes Splendens) And Emini Silvery Mole-Rats (Bathyergidae: Heliophobius Argenteocinereus Emini), Jestina V. Katandukila

Tanzania Journal of Science

Root-rats (Tachyoryctes splendens) and emini silvery mole-rats (Heliophobius argenteocinereus emini) are common subterranean rodents that are essential component of the ecosystem. They serve as prey for small to medium-sized carnivores and birds of prey but their role as hosts to parasites is limited. The present study, explored the Gastrointestinal Tract (GIT) contents of 40 rodents (20 T. splendens and 20 H. a. emini, respectively) to acquire information on parasites diversity of these mammals. The GIT contents from subterranean rodents in a sex ratio of 1:1 were examined under a compound microscope to isolate helminths. The isolated helminths belonged to the …

Meningioma Detection From Mri: A Comparative Study Of Deep Learning Models, A. H. Doha, Sandi Jennifer, Fatima Alabdaly

AUIQ Complementary Biological System

This study compares several deep learning models including DenseNet20, CNN, ResNet50, VGG19, EfficientNet-B3, MobileNetV2, NASNetMobile, Xception to automatically classify brain meningiomas from MRI scans. A public dataset was used to evaluate the proposed model. All models were trained, validated, and tested on this dataset. Because meningiomas are common and manual reads, the goal is to speed and standardize diagnosis. Our main contribution is pairing a high-accuracy classifier with deep learning models. Among all models, DenseNet201 performed best, reaching 0.991% accuracy and reliably separating tumorous from non-tumorous MRIs. Future work will expand the dataset and incorporate stronger segmentation to further boost …

Hiv-1 Tat-Driven Glutamate Dysregulation: Implications For Cognitive Impairment In Hand, Brenna Duffy, Michael Nonnemacher, Sandhya Kortagere

Kimmel Cancer Center Faculty Papers

PURPOSE OF REVIEW: HIV-1-associated neurocognitive disorders (HAND) manifest in 15% to 50% of people with HIV, impairing learning and memory and executive function. The chronic generation of HIV-1 Transactivator of transcription (Tat) likely contributes to HAND via direct neuronal toxicity and glial-mediated toxicity. This review summarizes our current understanding of how chronic Tat generation from microglia and astrocytes promote glutamate excitotoxicity.

RECENT FINDINGS: In recent years, the indirect effects of Tat through the activation of glial cells have gained significant interest. This review highlights microglia and astrocytes as HIV-1 reservoirs that release Tat protein in the central nervous system. Specific …

Towards Sustainable Marine Education: Assessing High School Students’ Understanding Of Conservation Concepts, Cheng-Chieh Chang, Tsuyoshi Sasaki, Ya-Yin Liao, Ling-Ya Hung, Liang-Ting Tsai

Journal of Marine Science and Technology–Taiwan

This study assesses the concepts of marine resource conservation and sustainability among Taiwanese high school students by developing a Chinese version of the Marine Resources Conservation and Sustainability Scale. Grounded in the cognitive, socio-emotional, and behavioral learning objectives outlined in UNESCO’s Education for Sustainable Development Goals (ESDGs), the scale targets students aged 16 to 18 to examine gender differences in marine knowledge and conservation concepts. The scale comprises 31 items across four dimensions: the first section evaluates marine knowledge, while the remaining dimensions utilize a four-point Likert scale to assess cognitive, socio-emotional, and behavioral learning objectives, respectively. The results indicate …

(Re)Claiming Homeplace: Dance As Embodied Resistance & Healing For Health & Spatial Justice, Preeya G. Kannan

University Honors Theses

This thesis argues that dance functions as both an upstream public health intervention and a form of radical spatial resistance; creating healing, agency, and belonging among historically marginalized communities. Drawing on bell hooks' concept of homeplace, Jasbir Puar's assemblage theory, Nancy Krieger's eco-social theory, and Katherine McKittrick's Black geographies, I position the body as both an ecological and political landscape shaped by histories of colonialism, racial capitalism, displacement, and resilience.

Through this community-based participatory research study, Homeplace, I explore dance as a form of social prescription that centers liberation rather than pathology. Movement is often reduced in public health discourse …

Why Student Research Matters: A Graduate Student’S Perspective, Jessica L. Hensley

The Journal of Integrative Physiology

Research fundamentally changes how students engage with knowledge. Rather than memorizing established facts, students learn to question, analyze, and contribute to the body of knowledge within their field (1). This shift supports deeper understanding and promotes critical thinking skills by encouraging active construction of knowledge (1). It also exposes students to the process of engaging with scientific literature and integrating information within the context of their research (2).

In my experience, research requires individuals to confront uncertainty. Experiments do not always produce exact results, and methods can require modification. Through this process, students develop adaptability, persistence, and the ability to …

The Neuroprotective Effect Of Cdc42 Inhibition In The G93a Mutant Hsod1 Mouse Model Of Als, Andrea Koly

Undergraduate Theses, Capstones, and Recitals

Amyotrophic lateral sclerosis (ALS) is a progressive and fatal neurodegenerative disease characterized by the selective loss of upper and lower motor neurons, leading to muscle weakness, neuromuscular junction (NMJ) degeneration, paralysis, and eventual death due to respiratory failure. Despite extensive research, effective disease-modifying therapies remain limited, underscoring the need to identify novel molecular targets involved in ALS pathogenesis. Dysregulation of Rho family GTPases has been implicated in neurodegeneration, with Rac and Rho exerting opposing effects on neuronal survival; however, the role of the closely related GTPase Cdc42 remains poorly understood in ALS. In this study, the therapeutic potential of inhibiting …

Differential Roles Of Moesin And Vasp In Β-Catenin-Associated Networks During Zebrafish Heart Regeneration, Allen Rutman

Undergraduate Theses, Capstones, and Recitals

With cardiovascular disease remaining as the leading cause of mortality worldwide, it is largely due to the limited regenerative capacity of the human heart. However, zebrafish (Danio rerio) can fully regenerate cardiac tissue following injury, providing a valuable model for studying the mechanisms of heart repair. To investigate these mechanisms, we used proteomics methods in the past. Proximity labeling is a technique that identifies proteins based on their physical proximity to a protein of interest. It can be applied in organisms and in living cells (Roux et al., 2012; Cho et al., 2020). We used proximity labeling to characterize protein …

Allosteric And Uncompetitive Inhibition Of Yeast Alcohol Dehydrogenase Identified By Enzyme Kinetics, Dylan Jeremy Harrison, Jenny A. Cappuccio

IdeaFest: Interdisciplinary Journal of Creative Works and Research from Cal Poly Humboldt

Enzyme kinetics is a powerful technique used to quantify the rate at which an enzyme converts a substrate into a product and to identify potential inhibitors. In this study, kinetic analysis was employed to assess the activity of yeast alcohol dehydrogenase (YADH) in the presence and absence of sweeteners commonly found in alcoholic beverages. YADH catalyzes the oxidation of ethanol to acetaldehyde using NAD⁺ as a cofactor; the reduction to NADH can be monitored spectrophotometrically at 340 nm. Based on structural similarities to the ribose moiety of NAD⁺, it was hypothesized that sucrose and sucralose could directly interact with the …

Neuromuscular Responses During Vigorous Intensity Heart Rate-Clamp Cycling Exercise, Clara J. Mitchinson, Pasquale J. Succi, Brian Benitez, Djadmann Gustave, Minyoung Kwak, Erik Snell, Haley C. Bergstrom

Journal for Sports Neuroscience

Introduction: Electromyography (EMG) and mechanomyography (MMG) provide non-invasive assessments of neuromuscular function and fatigue during dynamic exercise. This study examined neuromuscular fatigue and cycling performance through changes in power output (PO), total work (TW), EMG and MMG, amplitude (AMP) and mean power frequency (MPF) during three, heart rate (HR)-Clamp trials in the vigorous range (77-95% HR_max). Methods: Ten men (mean±SD, 25.9±5.0yr, 74.9±15.0kg, HR_max=178±12b×min^-1) performed a cycle ergometer graded exercise test, followed by three separate HR-Clamp trials at 77%HR_max (HR_L, time to exhaustion [T_Lim]=58.5±4.6 min, range=45.3-60.0min), 86%HR_max (HR_{M …}

The Palm Farm Next-Door: Land Concessions, Livelihoods, And Nutrition In Plantation-Adjacent Communities, Julia Entwistle

Electronic Theses and Dissertations

Large-scale land concessions have reshaped agrarian landscapes globally, yet their implications for community land tenure, livelihoods, and nutrition remain unevenly understood. This dissertation examines how plantation agriculture and foreign investment have transformed land access, labor relations, and food systems in Liberia, a country with one of the highest proportions of land under concession globally. Guided by a political ecology framework, the study employs a mixed-methods design integrating policy analysis, in-depth interviews, and household surveys across two palm oil concession sites. The findings show that ambiguous land policies historically facilitated dispossession, while recent legal reforms have enabled communities to strategically mobilize …

Food System Science Lesson-1 For First Graders. Makhá Waókiyapi: Soil Helpers, Feeding The Land That Feeds Us (Lesson About Regenerative Agriculture), Arlene A. Abao, Madhav P. Nepal

iLEARN Teaching Resources

In this engaging Grade 1 Science adventure, students become "Earth Doctors" as they discover that the soil beneath their feet is a living, breathing community! Guided by the Lakota value of Mitákuye Oyás’iŋ (all are related), children will learn that Makhá (the Earth) is not just "dirt," but a relative that provides us with everything we need to live. Students will get their hands dirty investigating the "Soil Helpers"—the worms, roots, and tiny bugs that work like a team to keep the land healthy. They will explore the three golden rules of being a good neighbor to the Earth: Compost …

Food System Science Lesson-2 For First Graders. Makhózula Etáŋhaŋ Wiyútapi Ke: From Planting To Eating, Arlene A. Abao, Madhav P. Nepal

iLEARN Teaching Resources

Feeding the Land That Feeds Us In this delicious adventure, kids will trace the journey of their food from the deep, dark soil all the way to their lunch forks! They will discover that the land is like a giant, magical kitchen called Makhózula, where plants cook up energy using sunlight and "vitamins" from the soil. Students will learn that when we eat a crunchy carrot or a sweet berry, we are actually tasting the strength of the Earth and the warmth of the sun. Through stories of Wopila (gratitude), they’ll see that the food system is a big, …

A Computational Micropatterning Approach To Study Caveolae And Protein Localization In Vascular Biology, Andrew B. Grespin

Electronic Theses and Dissertations

Caveolae are specialized, flask-shaped membrane invaginations highly expressed in endothelium and dysregulated in atherosclerosis. Caveolae play a central role in buffering membrane tension, yet the principles governing their spatial organization remain elusive. Thus, we sought to generate the most comprehensive and systematic analysis of blood vessel caveolar spatial organization. However, cell culturing, the backbone of human-focused biological research, does not standardly do well to model physiologically relevant cell behaviors such as migration or polarity-based tissue formation, particularly for punctate proteins and structures like caveolae. Micropatterns are cell-adhesive shapes that biophysically confine cell(s) to a user defined geometry which stereotype organelle …

Comparison Of Bacteriological Culture And A Multiplex Real-Time Pcr Kit For The Detection Of Mastitis Pathogens In Small Ruminants, Ebru Karakaya Bi̇len, Serpi̇l Kahya Demi̇rbi̇lek, Bariş Güner, Öznur Yilmaz Koç, Ali̇ Ünver, Merve Yildiz, İrem Çakmak, Abdulkadi̇r Keski̇n

Turkish Journal of Veterinary & Animal Sciences

This study aimed to evaluate the applicability of a multiplex real-time PCR (rPCR) kit, originally developed for bovine mastitis, for detecting mastitis pathogens in small ruminants and reducing bacterial identification time compared with conventional bacteriological culture (BC). Milk samples were obtained from 175 sheep and 124 goats and included cases of clinical mastitis (n = 57) and subclinical mastitis (n = 242). With BC, bacterial growth was detected in 125 of 299 milk samples (41.8%), yielding a total of 169 bacterial isolates. The pathogens most frequently isolated by BC were coagulase-negative staphylococci (58.0%), followed by Streptococcus spp. (9.4%), Staphylococcus aureus …

Comparative Efficacy Of Controlled Internal Drug Release And Medroxyprogesterone Acetate Sponges On Ovulatory Follicle Growth In The Ovsynch Protocol During The Breeding Season In Cyclic Nili Ravi Buffaloes, Muhammad Binyameen, Ali Husnain, Saba Anwar, Muhammad Waseem, Muhammad Saleem

Turkish Journal of Veterinary & Animal Sciences

The objective of the experiment was to compare the dominant follicle size, growth rate of the dominant follicle, estrus response, plasma progesterone concentration, and pregnancy in Nili Ravi buffaloes synchronized by controlled internal drug release (CIDR; 1.38 g) and medroxyprogesterone acetate sponge (MPAS; 250 mg) in the ovulation synchronization (OvSynch) protocol. The experiment had a randomized complete block design. Buffaloes (n = 42) were blocked by parity, body weight, body condition score, and ovarian status, and, within the block, they were randomized to receive either CIDR or MPAS and that day was considered day 0 of the experiment. On days …