Digital Commons Network^™

Design And Application Of Hybrid Cyclic-Linear Peptide-Doxorubicin Conjugates As A Strategy To Overcome Doxorubicin Resistance And Toxicity, Saghar Mozaffari, David Salehi, Parvin Mahdipoor, Richard Beuttler, Rakesh Tiwari, Hamidreza Montazeri Aliabadi, Keykavous Parang

Pharmacy Faculty Articles and Research

Doxorubicin (Dox) is used for breast cancer, leukemia, and lymphoma treatment as an effective chemotherapeutic agent. However, Dox use is restricted due to inherent and acquired resistance and an 8-fold increase in the risk of potentially fatal cardiotoxicity. Hybrid cyclic-linear peptide [R5K]W7A and linear peptide R5KW7A were conjugated with Dox through a glutarate linker to afford [R5K]W7A-Dox and R5KW7A-Dox conjugates to generate Dox derivatives. Alternatively, [R5K]W7C was conjugated with Dox via a disulfide linker to generate [R5K]W7C–S–S-Dox conjugate, where S–S is a disulfide bond. Comparative antiproliferative assays between conjugates [R5K]W7A-Dox, [R5K]W7C–S–S-Dox, linear R5KW7A-Dox, the corresponding physical mixtures of the peptides, …

Intermittent Bolus Compared With Continuous Feeding Enhances Insulin And Amino Acid Signaling To Translation Initiation In Skeletal Muscle Of Neonatal Pigs, Agus Suryawan, Samer W El-Kadi, Hanh V Nguyen, Marta L Fiorotto, Teresa A Davis

Children’s Nutrition Research Center Staff Publications

Background: Nutrition administered as intermittent bolus feeds rather than continuously promotes greater protein synthesis rates in skeletal muscle and enhances lean growth in a neonatal piglet model. The molecular mechanisms responsible remain unclear.

Objectives: We aimed to identify the insulin- and/or amino acid-signaling components involved in the enhanced stimulation of skeletal muscle by intermittent bolus compared to continuous feeding in neonatal pigs born at term.

Methods: Term piglets (2-3 days old) were fed equal amounts of sow milk replacer [12.8 g protein and 155 kcal/(kg body weight · d)] by orogastric tube as intermittent bolus meals every 4 hours (INT) …

Lingnan Gardeners Newsletter (No. 57) = 彩園通訊 (第57期), Lingnan Gardeners, Centre For Cultural Research And Development, Lingnan University

Lingnan Gardeners Newsletter 嶺南彩園通訊

No abstract provided.

Don't Sell Them Short, There's More To Bacterial Natural Products Than Antibiotics, Alison Clare Domzalski

Dissertations, Theses, and Capstone Projects

Recent genomic studies of microbiomes have revealed an overwhelming number of biosynthetic genes of unknown function. Most of these “cryptic” biosynthetic genes are not expressed in laboratory monocultures of individual microbes. Thus, there remains tremendous untapped potential for natural products discovery. Here we employ mixed microbial culture (MMC) as a simple yet powerful approach to awaken cryptic biosynthetic gene clusters. Our preliminary studies demonstrated that arrays of metabolites could be induced in MMCs upon environmental cues, such as surface adhesion. Using this system, we have screened, identified, and isolated bioactive bacterial metabolites, which were characterized structurally and biologically. Of the …

Understanding The Mechanistic Roles Of Integrin Alpha 6 In Tumor Development Using A Humanized Zebrafish (Danio Rerio) Model System, Ashley B. Williams

Honors College Theses

Present day cancer incidence and mortality rates indicate the need for effective cancer diagnostic tools and targeted cancer therapeutic strategies. Recent studies have focused on the biological pathways of cells and tumor microenvironments to identify putative biomarkers and potential drug targets as diagnostic and therapeutic tools. Human integrins, adhesion receptors, have become the focal points in these studies, specifically Integrin Alpha 6 (ITGA6) which has been implicated in major tumor progression roles: metastasis and angiogenesis. These characteristics make ITGA6 an excellent candidate for potential drug or diagnostic target, however, the mechanism by which ITGA6 facilitates tumor progression remains unclear. Cell …

Cross-Frequency Coupling Explains The Preference For Simple Ratios In Rhythmic Behaviour And The Relative Stability Across Non-Synchronous Patterns, Dobromir Dotov, Laurel Trainor

Journal Articles

Rhythms are important for understanding coordinated behaviours in ecological systems. The repetitive nature of rhythms affords prediction, planning of movements and coordination of processes within and between individuals. A major challenge is to understand complex forms of coordination when they differ from complete synchronization. By expressing phase as ratio of a cycle, we adapted levels of the Farey tree as a metric of complexity mapped to the range between in-phase and anti-phase synchronization. In a bimanual tapping task, this revealed an increase of variability with ratio complexity, a range of hidden and unstable yet measurable modes, and a rank-frequency scaling …

Regulation Of Host-Microbe Interactions In Autoimmunity And Antiviral Immunity By Cytosolic Nucleic Acid Sensing And Interferon Signaling, Derek Jerome Platt

Arts & Sciences Graduate Student Theses and Dissertations

Cytosolic nucleic acid sensing and interferon (IFN) signaling are central to the host immune response to microbial pathogens. However, dysregulation of immunological pathways such as these can result in devastating autoimmune disease. In order to provide a robust immune response to pathogen without causing harm to self, the host immune system must engage in a delicate balancing act, interacting with microbes and determining whether they are commensal or pathogenic. The cGAS-STING pathway is a key regulator of host-microbe interactions by cytosolic nucleic sensing and IFN signaling. Loss of function in the cGAS-STING pathway leads to increased susceptibility to pathogenic threats, …

The Beneficial Effects Of The Gut-Derived Metabolite Trimethylamine N-Oxide On Functional Β-Cell Mass, Emily Suzanne Krueger

Theses and Dissertations

Elevated serum levels of trimethylamine N-oxide (TMAO) were first associated with increased risk of cardiovascular disease (CVD) 10 years ago. Research has since defined that serum TMAO accumulation is controlled by the diet-microbiome-liver-kidney axis. Choline related nutrients are consumed in excess during over-nutrition from a Western diet. The resultant elevated serum TMAO is investigated across various chronic metabolic diseases and many tissue types. While TMAO is most clearly linked to CVD mechanisms in vascular tissue, its molecular effects on metabolic tissues are unclear. Here we report the current standing of TMAO research in metabolic disease context across relevant metabolic tissues …

Understanding The Pathogenesis Of Renal Medullary Carcinoma, Melinda Soeung

Dissertations and Theses (Open Access)

Renal medullary carcinoma (RMC) is a lethal cancer that predominantly affects young individuals with sickle cell trait (SCT). It is not currently understood why RMC only affects certain individuals with SCT. We found that patients with RMC more frequently participated in high-intensity exercise than matched controls. Using mouse models of SCT, we demonstrated the significant increase of renal hypoxia in the right kidney following high- but not moderate-intensity exercise. We also demonstrated in cell culture studies that SMARCB1 is ubiquitinated for proteasome-mediated degradation in hypoxia, and the re-expression of SMARCB1 leads to compromised proliferation in renal cells specifically in the …

Integrating Underutilized Black Volunteers In 4-H Youth Development Programs, Maurice Smith Jr., Shannon Wiley

Journal of Extension

4-H Youth Development prides itself on providing essential resources to reach underserved minority populations. 4-H provides programming and professional development for volunteers to include diverse hands-on training, and cultural competency workshops. This article provides best practices for the inclusion of African American volunteers in 4-H programming efforts that could help extension educators better understand the need to include minority volunteer roles and responsibilities. These strategies include strengthening diverse volunteer make-up, increasing participation and trust among African American youth, and engaging volunteers working in educational organizations that could provide real world experiences for youth.

Thanatechnology And The Living Dead: New Concepts In Digital Transformation And Human-Computer Interaction, Vural Özdemir, Simon Springer, Arif Ylldlrlm, Sehmus Biçer, Asllgül Kendirci, Semra Sardaş, Hakan Klllç, Nezih Hekim, Tanja Kunej, Kazlm Yalçln Arga, Kevin Dzobo, Wei Wang, Marius Geanta, Angela Brand, Mustafa Bayram

Research outputs 2014 to 2021

In a digital society, shall we be the authors of our own experience, not only during our lifetime but also after we die? We ask this question because dying and bereavement have become even harder, and much less private, in the digital age. New big data-driven digital industries and technologies are on the rise, with promises of interactive 3D avatars and storage of digital memories of the deceased, so they can continue to exist online as the "living dead"in a digital afterlife. Famous rock and roll icons like Roy Orbison, Frank Zappa, Ronnie James Dio, and Amy Winehouse have famously …

Conclusion: Female Leaders Using Coercive Power Motivate Subordinates, Mary Kovach

The Journal of Values-Based Leadership

This manuscript advances prior research (Blau, 1964; Elangovan & Xie, 1999; French & Raven, 1959; Goodstadt & Hjelle, 1973; Hegtvedt, 1988; Randolph & Kemery, 2011; Zigarmi, Peyton Roberts, & Randolph, 2015) and capitalizes on supervisory skills using power dynamics within the workplace, by investigating employee effort resulting from gender dissimilar supervisor-employee dyads and employee locus of control. To offer a more focused approach, this is an evaluation specifically on reward and coercive power derived from French and Raven’s (1959) five power bases. This manuscript proposes that the motivation levels of employees change, based on their locus of control and gender. …

The Influence Of Gender Approaches On Education Efficiency, G. Eshchanova

Irrigation and Melioration

The given article covers the influence of gender approaches on education efficiency on the basis of statistical and scientific data, and the need to rely on the basis of the necessary knowledge for the ecologically sustainable development of women's knowledge, competence and entrepreneurship are shown. The educational process based on Gender differences and equality contributes significantly to the development of society.

The Effect Of Fluid Flow Shear Stress And Substrate Stiffness On Yes-Associated Protein (Yap) Activity And Osteogenesis In Murine Osteosarcoma Cells, Thomas R. Coughlin, Ali Sana, Kevin Voss, Abhilash Gadi, Upal Basu-Roy, Caroline M. Curtin, Alka Mansukhani, Oran D. Kennedy

Publications and Research

Osteosarcoma (OS) is an aggressive bone cancer originating in the mesenchymal lineage. Prognosis for metastatic disease is poor, with a mortality rate of approximately 40%; OS is an aggressive disease for which new treatments are needed. All bone cells are sensitive to their mechanical/ physical surroundings and changes in these surroundings can affect their behavior. However, it is not well understood how OS cells specifically respond to fluid movement, or substrate stiffness—two stimuli of relevance in the tumor microenvironment. We used cells from spontaneous OS tumors in a mouse engineered to have a bone-specific knockout of pRb-1 and p53 in …

Effect Of The Lysosomotropic Agent Chloroquine On Mtorc1 Activation And Protein Synthesis In Human Skeletal Muscle, Michael S. Borack, Jared M. Dickinson, Christopher S. Fry, Paul T. Reidy, Melissa M. Markofski, Rachel R. Deer, Kristofer Jennings, Elena Volpi, Blake B. Rasmussen

All Faculty Scholarship for the College of Education and Professional Studies

Background

Previous work in HEK-293 cells demonstrated the importance of amino acid-induced mTORC1 translocation to the lysosomal surface for stimulating mTORC1 kinase activity and protein synthesis. This study tested the conservation of this amino acid sensing mechanism in human skeletal muscle by treating subjects with chloroquine—a lysosomotropic agent that induces in vitro and in vivo lysosome dysfunction.

Methods

mTORC1 signaling and muscle protein synthesis (MPS) were determined in vivo in a randomized controlled trial of 14 subjects (10 M, 4 F; 26 ± 4 year) that ingested 10 g of essential amino acids (EAA) after receiving 750 mg of chloroquine …

Component Causes Of Infectious Bovine Keratoconjunctivitis—Non-Moraxella Organisms In The Epidemiology Of Infectious Bovine Keratoconjunctivitis, John Dustin Loy, Kristin A. Clothier, Gabriele Maier

School of Veterinary and Biomedical Sciences: Faculty Publications

• Non-Moraxella organisms are associated with infectious bovine keratoconjunctivitis (IBK).

• Mycoplasma bovoculi can cause conjunctivitis and has a potential role in IBK pathogenesis.

• Other Non-Mycoplasma agents can cause disease that resembles IBK but is clinically different.

• Genomics and molecular technology are advancing research in this area.

• Classification and determination of pathogenesis potential of these organisms may be better understood through metagenomics and whole genome sequencing.

Androgen Receptor, Although Not A Specific Marker For, Is A Novel Target To Suppress Glioma Stem Cells As A Therapeutic Strategy For Glioblastoma, Nan Zhao, Fei Wang, Shaheen Ahmed, Kan Liu, Chi Zhang, Sahara J. Cathcart, Dominick J. Dimaio, Michael Punsoni, Bingjie Guan, Ping Zhou, Shuo Wang, Surinder K. Batra, Tatiana Bronich, Tom K. Hei, Chi Lin, Chi Zhang

School of Biological Sciences: Faculty Publications

Targeting androgen receptor (AR) has been shown to be promising in treating glioblastoma (GBM) in cell culture and flank implant models but the mechanisms remain unclear. AR antagonists including enzalutamide are available for treating prostate cancer patients in clinic and can pass the blood–brain barrier, thus are potentially good candidates for GBM treatment but have not been tested in GBM orthotopically. Our current studies confirmed that in patients, a majority of GBM tumors overexpress AR in both genders. Enzalutamide inhibited the proliferation of GBM cells both in vitro and in vivo. Although confocal microscopy demonstrated that AR is expressed but …

Ranking The Counties In Ease Azarbaijan-Iran Based On Agriculture Development Criteria Using Copras Model, Razieh Pourdarbani, Asghar Pashazadeh

Emirates Journal for Engineering Research

Understanding inequality and imbalances within different geographical areas (country, province and city), and policymaking to reduce inequalities are among the key tasks of the administrators in geographical development sector. Therefore, it is necessary to identify level of development and underdevelopment of agricultural areas in order to achieve sustainable agricultural development. A list of 36 indices including 10 agronomic indices, 7 horticultural indices, 8 livestock indices and 11 mechanization indices was prepared using Statistical Yearbook of 2016. The agricultural ranking of 21 counties was made using the Copras model. Also counties clustered using cluster analysis method included in SPSS software and …

Intangible Cultural Heritage: A Benefit To Climate-Displaced And Host Communities, Gül Aktürk, Martha B. Lerski

Publications and Research

Climate change is borderless, and its impacts are not shared equally by all communities. It causes an imbalance between people by creating a more desirable living environment for some societies while erasing settlements and shelters of some others. Due to floods, sea level rise, destructive storms, drought, and slow-onset factors such as salinization of water and soil, people lose their lands, homes, and natural resources. Catastrophic events force people to move voluntarily or involuntarily. The relocation of communities is a debatable climate adaptation measure which requires utmost care with human rights, ethics, and psychological well-being of individuals upon the issues …

The Role Of Med12 In Adipogenesis Of Human Adipose Stem Cells, Onyekachi Chinelo Idigo

Master's Theses

The interest in adipogenesis stems from the high rate of obesity in the world and the motivation to better understand the process of cell fate in relation to human diseases. Mutations in MED12 lead to developmental disorders and certain malignancies. To date, specific roles of Mediator and MED12 in cell state regulation are unclear, nevertheless, they are critical for understanding stem cell regulation and enhancement of their clinical applications. This study investigates the role of MED12 in relation to the Mediator complex kinase domain to better understand transcriptional control of adipogenesis in hASCs. In this study, MED12 expression was diminished …

Molecular And Genetic Studies Of Robo2 Transcriptional Regulation In The Central Nervous System Of Drosophila Melanogaster, Muna Abdal Rahim Abdal Rhida

Graduate Theses and Dissertations

Drosophila Robo2 axon guidance receptor is a member of the evolutionarily conserved Roundabout (Robo) protein family that is involved in directing axons that cross the midline to the other side of the animal body. Robo2 roles mainly depend on two factors: The functional domains of the Robo2 protein, which is extensively studied, and the dynamic transcription of robo2 in various subsets of cells throughout embryogenesis which is not fully understood. Thus, knowing robo2 enhancers that transcriptionally regulate robo2 during embryogenesis is significant. To investigate robo2 potential enhancers, we screened 17 transgenic lines of Drosophila that were generated by Janelia Research …

Lingnan Gardeners Newsletter (No. 55) = 彩園通訊 (第55期), Lingnan Gardeners, Centre For Cultural Research And Development, Lingnan University

Lingnan Gardeners Newsletter 嶺南彩園通訊

No abstract provided.

Vertical Transmission Of Zika Virus In An Opossum Model, Dionn Carlo Silva

Theses and Dissertations

This study will identify Brazilian ZIKV infection in the offsprings of mated, inoculated Monodelphis domestica females. Unlike non-human primates (NHP) and transgenic mice, M. domestica provides an opportunity to study ZIKV infection in a novel animal model possibly useful for in-utero studies. M. domestica has also been used in cancer and dietary research with varying degrees of success. ZIKV infection will be identified using antibody staining and in situ hybridization. The expected result of this study is that ZIKV will be identified in various tissues and reveal that ZIKV is transmitted vertically in this animal model. The significance of this …

Optimization Of Broken Rice Consumption Through Understanding The Confluence Of Sensory And Economic Preferences, Matthew Greer Richardson

Graduate Theses and Dissertations

The authors were perplexed by the seeming dichotomy between the majority 70% of Haitian consumers being chronically food insecure, but still preferring to pay premium international market prices for long-grain rice that constitutes 30% of the calories in this poorest nation in the Western Hemisphere. The aim of this study was to determine the optimal percent broken rice in regards to price specifically to Haiti. Optimization between quality and price point is seen as the best rice for the price, and can be found through combining individual preferences with willingness to pay. Broken rice (brokens) garner a significantly discounted price …

In The Spirit Of St. Peter Claver: Social Justice And Black Catholicism In San Antonio, Philip Lampe Ph.D.

Verbum Incarnatum: An Academic Journal of Social Justice

The editors want to take the space reserved for the abstract to say that this is the final piece of research that Phil Lampe completed before his passing. We publish it here posthumously in tribute to Phil’s tireless work for social justice, as editor of Verbum Incarnatum, as researcher of social-justice efforts in South Texas and Mexico, and as an educator committed to inspiring students to pursue justice in their lives outside the academy.

Social Justice In The Cigar Factory: The Finck Cigar Strikes, 1933-1935, Roger Barnes

Verbum Incarnatum: An Academic Journal of Social Justice

No abstract provided.

A Participatory Action Research Study Of Police Interviewing Following Crisis Intervention Team Training, Maria Felix-Ortiz, Catherine Steele, Marisa Deguzman, Georgen Guerrero, Melissa Graham

Verbum Incarnatum: An Academic Journal of Social Justice

Estimates vary, but a third to one half of individuals shot and killed by police have a mental illness or disability, and many who are taken into custody languish in county jails where no treatment for their illness is available. The Crisis Intervention Team (CIT) model is an increasingly important adjunct to U.S. police training because it de-escalates tense situations, diverts people with mental illness away from jail and into treatment, and can reduce the risk of civilian deaths during a police encounter. As such, it is a strategy for reducing the social injustice of incarceration or deaths of people …

Promises Endure: Historical Views Of Nursing Faculty, Laura R. Muñoz

Verbum Incarnatum: An Academic Journal of Social Justice

Lessons learned from the history of an organization are valuable. This is especially true for an organization with the legacy held by the Ila Faye Miller School of Nursing and Health Professions at the University of the Incarnate Word. Memories recounted by nursing faculty were collected to enhance information provided in the two-volume chronicle written by Sister Patrice Slattery in 1995 entitled, “Promises to Keep” and the last history of the school, “The Story of One School of Nursing” written by Sister Charles Marie Frank in 1976.

The One – Way (Agri)Cultural Mirror: A Case Study Of How Young Agriculturalists Understand And Experience Culture, Janiece M. Pigg

LSU Master's Theses

As the global economy continues to transform how society operates, cultural competence has become a buzzword in education, professional development, research, government, and healthcare (Gay, 1994; Gallus et al., 2014). Cross et al. (1989) developed the most accepted definition of cultural competence: “a set of congruent behaviors, attitudes, and policies that come together in a system, agency, or among professionals and enable that system, agency, or those professionals to work effectively in cross-cultural situations” (p. 13).

Despite this, little to no research has been devoted to understanding cultural competence in agriculture. Thus, a need emerged to describe the cultural competence …

Serve-Specific Core Self-Evaluations: A Predictive Validation Study, Brandi Renee Forgione

Masters Theses & Specialist Projects

Core Self-Evaluations (CSE) is a broad personality trait used by industrial organizational psychologists to predict important organizational outcomes such as job performance and employee satisfaction. CSE comprises four elements: generalized self-efficacy, self-esteem, locus of control, and neuroticism. Task-specific versions of CSE have been proposed to better predict task-specific performance than general CSE. Accordingly, Serve-Specific Core Self- Evaluations (SS-CSE) was adapted specifically to predict serve performance of intercollegiate volleyball players. In this study, I explored the predictive validity of SS-CSE for serve performance by expanding a previous study that used a concurrent validation approach. Results indicated that SS-CSE scores collected pre-season …