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Analysis Of Asexual Reproduction Dugesia Dorotocephala, Harvey Dee Mecham

Theses and Dissertations

Genetic control of decapitation-induced fission in planaria is mediated by stimulator and inhibitor molecules. Resolution of the two factors was accomplished by filtration chromatography of whole planaria extract. The stimulator (M.W. 2500 to 3500) was purified with ion exchange chromatography and increases the multiple fission rate. The inhibitor (M.W. 20,000 to 50,000) is non-dialyzable, heat labile and is inactivated unless kept frozen. Storage at 4°C results in a loss of inhibitory capacity with a concomitant stimulator increase, suggesting a protein-stimulator complex as the inhibitor. In light of a demonstrated animal intercommunication system, the inhibitor-stimulator complex is hypothesized as part of …

Physiological Regulation Of Cyclic Flowering In Flax, Aurora Salazar Hovland

Electronic Theses and Dissertations

The flowering pattern of flax (Linum usitatissimum, L.) is cyclic in nature with periods of blossoming separated by periods of rest. To study the regulation of flowering cycles, four experiments were conducted. The first two dealt with nutritional aspects of cyclic flowering by imposing environmental stresses which conceivably might regulate flowering pattern nutritionally. The third and fourth experiments dealt with regulation from hormonal systems and included in vitro regulation of bud growth by growth regulators as well as characterization of endogenous hormonal systems by fluorimetry and bioassays. In the first experiment, the effects of light intensity, temperature, nitrogen and defoliation …

The Effects Of Desoxycorticosterone, Hydrocortisone, And Prednisone On Myogenesis In Vitro, Gregory K. Nienaber

Masters Theses

In order to obtain proper muscle development in tissue culture, certain conditions must prevail. There is a delicate balance of factors, and elimination of even one of these factors leads to poor and improper development. Muscle cells grown in the absence of fibroblasts usually fail to differentiate. Therefore, proper development depends upon the presence of two cell types: myoblasts and fibroblasts. Investigators have demonstrated that the fibroblasts are necessary in that they produce collagen which is necessary for proper muscle development. The present study has shown that desoxycorticosterone, hydrocortisone, and prednisone at concentrations of 10, 1, and 0.1 ug/ml of …

Critical Factors In The Management Of Liver Abscesses, Melvin A. Block, Hubert M. Allen

Henry Ford Hospital Medical Journal

Newer diagnostic techniques, including radioisotope liver scans, bacteriologic culture procedures for anaerobes, serologic tests for amebiasis, ultrasonic echograms, and hepatic arteriography, permit more specific identification and localization of hepatic abscesses. Large pyogenic abscesses are of particular Importance to surgeons; are frequently caused by anaerobic organisms; and require early, direct, adequate, and sufficiently prolonged surgical drainage. The clinician should be alert also to recognition and management of the primary lesion responsible for the hepatic abscess, associated abscesses elsewhere, and the possible presence of an underlying liver neoplasm.

Phytoplankton Composition At Lake Drummond In The Dismal Swamp, Virginia, William Howard Poore Jr.

Biological Sciences Theses & Dissertations

Abstract unavailable.

Phytoplankton Periodicity In Shanty Hollow Lake, Warren County, Kentucky, David Hill

Masters Theses & Specialist Projects

A study of the phytoplankton periodicity and its correlation with chemicophysical parameters, diel variation of the plankton, and the cyclomorphotic variation of Ceratium hirundinella was made in Shanty Hollow Lake, Warren County, Kentucky, from October, 1969, to August, 1970.

Phytoplankton samples were collected at zero and six feet with a Kemmerer sampler (31 October through 8 December) and with a ten -liter Birge-Juday plankton trap (23 December through 9 August). Water samples for the chemicophysical determinations were collected with a Kemmerer sampler. The phytoplankton samples were preserved with 5% formalin until they were enumerated. The chemicophysical determinations (pH, conductivity, dissolved …

85th Annual Report Of The Nebraska Agricultural Experiment Station. July 1, 1970 To June 30, 1971

Agricultural Research Division: News and Annual Reports

Contents

Foreword

Administration and Staff

Nebraska Agricultural Experiment Station Research Projects

Experiment Station Publications . .

Publications of the Staff . . . . .

Report of Income and Expenditures

Issued January 1972, 1500

The 85th Annual Report of the Nebraska Agricultural Experiment Station contains a list of all active projects during July 1, 1970 to June 30, 1971, a list of Station publications, staff members, and a financial report. Information derived from the various research projects is published in bulletins, circulars, scientific and trade journals, in the Nebraska Farm, Ranch and Home Quarterly and as mimeographed departmental reports. Station …

Factors Affecting Pollination Of Onions In Idaho During 1969, William P. Nye, G. D. Waller, N. D. Waters

All PIRU Publications

No abstract provided.

Characterization Of Deoxyribonucleic Acid From Actinomycetes, Lynn William Enquist

Theses and Dissertations

Deoxyribonucleic acid (DNA) analyses were used to assess on a molecular level, the relationships among representatives of the genera Streptomyces, Nocardia and Mycobacterium. The methods developed in this study have been used for routine analysis of DNA from a large number of actinomycetes and have given reliable and reproducible data.

DNA isolated from various actinomycetes was characterized by buoyant density determinations in CsCl from which the mole fraction guanine plus cytosine (GC) content was calculated. All the streptomycete DNA preparations studied had buoyant densities in the range of 1.7287 to 1.7312 g cm^-3 which corresponded to GC compositions of …

Algal Growth And Decomposition: Effects On Water Quality, Edward G. Foree, John S. Tapp Jr.

KWRRI Research Reports

The chemical composition of algae grown in batch culture depends mainly on environmental conditions, nutrient availability, presence of predators, cell age, and species. The effects of nutrient availability and cell age on the composition of three unialgal cultures (algae + bacteria) and one hetergeneous culture (algae + bacteria + microscopic animals) were evaluated. The cultures were grown in batch culture under both nutrient-abundant and nutrient deficient conditions and the changes in compositions were observed. Luxurious uptake where nutrients are incorporated into cellular protoplasm at levels greater than those necessary for growth, and super-luxurious uptake, where some nutrients are stored rather …

83rd Annual Report Of The Nebraska Agricultural Experiment Station

Agricultural Research Division: News and Annual Reports

Contents:
Foreword
Administration and Staff
Nebraska Agricultural Experiment Station Research Projects
Experiment Station Publications
Publications of the Staff
Report of Income and Expenditures

Aqueous Microbiology: The Possible Role Of Cell Wall Deficient Bacteria In Uveitis, Philip C. Hessburg, Lioa H. Mattman, Carolyn Barth, Lloyd T. Dutcheshen

Henry Ford Hospital Medical Journal

Samples of aqueous humor were obtained from the anterior chamber of patients with uveitis. Bacterial L-forms, or cell wall deficient forms, were recovered from 11 of 19 eyes with unexplained uveitis. These bacterial variants were cultured on newer, highly specialized media and stained with acridine orange. This stain demonstrates fluorescence of nucleic acids under UV microscopy.

Ultrastructural Investigations Of Tilletia Caries Teliospores, James Vincent Allen

Theses and Dissertations

The purpose of this study was to investigate the ultrastructure of Tilletia caries teliospores. Specimens were prepared for viewing in the electron microscope by freeze-etching and thin-sectioning techniques. Thin-sectioning techniques gave excellent results with the teliospore walls and the lipid bodies within the teliospore but failed to adequately fix the membranes and internal organelle systems. Micrographs of Tilletia caries teliospores prepared using thin-sectioning show three major wall layers and two partition wall layers and abundant lipid bodies. The freeze-etch method of spore preparation proved superior to the thin-sectioning used in this study to reveal the internal organelle systems of the …

Effect Of Foliar Application Of Urea And Ammonium Nitrate On The Dry Weight And Protein Content Of Maize Plants, Alvaro Fiallos

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Urea and ammonium nitrate were applied to leaves of maize plants growing in growth chambers on nutrient solutions containing three different concentrations of ammonium nitrate. Dry weights, and the soluble protein contents of leaves, sterns and roots we remeasured.

Both urea and ammonium nitrate did increase the dry weights of leaves and stems when ammonium nitrate was used in the nutrient solutions (0.5 and 2.5 mM/ liter). When nitrogen was not used in the nutrient solutions, no increments of dry weight occurred. The protein contents of leaves were increased for plants in the same nutrient solutions that produced increases in …

A Preliminary Survey Of The Algal Flora Found On Tree Bark In East-Central Illinois, Marion E. Rohlinger

Masters Theses

No abstract provided.

Publications Of The Staff Of The Henry Ford Hospital And The Edsel B. Ford Institute For Medical Research

Henry Ford Hospital Medical Journal

No abstract provided.

Oral Bandage Tissue Reaction In Dogs, John Rozema

Loma Linda University Electronic Theses, Dissertations & Projects

This experiment was designed to evaluate an oral surgical dressing which may be used as an aid to healing tissue. The parameters of evaluation were directed toward wound healing enhancement, duration, and stability of the bandage. A histological evaluation of the surgical wounds protected with the bandage as compared to the control wounds was made.

The surgeries were performed on eight dogs in a double controlled experiment and were in the form of bilateral maxillary gingivectomies and mandibular molar tooth extractions. The surgery sites on the left were covered with the Orahesive Bandage while the right side remained exposed to …

The Nature Of Bone Resorption And Formation In Normalcy And Disease, Bruce N. Epker, Harold M. Frost

Henry Ford Hospital Medical Journal

Classically, Howship's lacunae and osteoid seams or tissue have been used for microscopic identification of sites of bone osteoclastic and osteoblastic cell activity respectively. Recent transmitted light and blue light fluorescence microscopic study of fresh, undemineralized, specially stained and tetracycline labeled bone specimens have supported this use of these parameters of bone resorption and formation. Reports that the osteoclast is not required for bone resorption are evaluated here; it is concluded that no evidence exsists for resorption of bone tissue without osteoclasts. This does not apply to the perilacunar phenomena observed in certain well-defined situations.

Infection In The Newborn Infant And Its Association With Prolonged Rupture Of The Amniotic Membranes, Philip J. Howard, A. Robert Bauer

Henry Ford Hospital Medical Journal

Infections in newborn infants should be suspected and treatment started whenever birth is prolonged following rupture of the membranes. These studies show that risk increases as rupture time gets longer. Selected antibiotics at proper dosages are recommended as preventive measures.

Comparison Of Two Cultures For Use In Preacidified Skimmilk For Cottage Cheese Manufacture, Daryl D. Boddicker

Electronic Theses and Dissertations

Curd formation in a cultured dairy product is brought about by the addition of a bacterial culture capable of producing lactic acid from lactose. The primary function of this culture is to produce sufficient lactic acid to cause coagulation of the caseinate system of skimmilk. A secondary function of the culture is the production of flavor compounds considered to be desirable in these products. The flavor compounds produced by the normal growth of citric acid fermenting organisms are water soluble, and are largely removed during washing of the Cottage cheese curd. Therefore, flavor compounds are normally added to the cheese …

80th Annual Report: Nebraska Agricultural Experiment Station

Agricultural Research Division: News and Annual Reports

Contents:
Foreword
Administration and Staff
Research Projects
Experiment Station Publications
Publications of the Staff
Financial Report

A Cytological Study Of Certain Life Cycle Stage Of Eimeria Bovis, John V. Ernst

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Although much research has been done on the bovine coccidia little has been reported on the cytological nature of the different stages. Few descriptions of cytoplasmic structures occur in the literature. Because of the impermeability of the oocyst wall a definitive study of the nucleus in the unsporulated oocyst has not been made, nor have the nuclear changes during the two successive divisions which occur during the process of sporulation been described.

Physio-Chemical Changes In Relation To The Rest Period Of Peach And Apricot Trees, Hussein Ibrahim El-Mansy

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Many plants require exposure to low temperature for a certain period during each year. This was determined more than a century ago. Understanding this phenomenon, known as the rest period, is part of a larger problem of understanding the factors which determine the ability of an organism to grow. The property of rest is ultimately a property of the actively growing cells, rather than that of the cells involved with protection and support.

The Liver Photoscan In The Recognition And Management Of Abdominal Masses, M. A. Block, B. M. Schuman, W. R. Eyler, J. P. Truant, L. A. Dusault

Henry Ford Hospital Medical Journal

No abstract provided.

Evaluation Of Two Mucolytic Agents As Digestants For The Isolation Of Mycobacteria, J. P. Truant, R. Burncon

Henry Ford Hospital Medical Journal

No abstract provided.

Hyperparasitism, Michael G. Boosalis

Department of Plant Pathology: Faculty Publications

Much of the research on hyperparasitism has been of a descriptive nature, based primarily on studies with dual cultures in synthetic media. The main contributions from these investigations concern the host range of the parasite, the mode of penetration and infection, and the morphological changes of the host and the parasite resulting from parasitism.

More recent studies on hyperparasitism emphasize the effect of environmental factors, especially nutrition, on the susceptibility of the host. Research on the physiology of hyperparasitism has been limited. Nevertheless, this important aspect of the problem should continue to receive increased attention as hyperparasitism is extremely amenable …

Cytological Evidence For Reciprocal Introgression In Agropyron Trachycaulum And Agropyron Spicatum, Laval M. Pitts

Theses and Dissertations

Agropyron trachycaulum (Link) Malte and Agropyron spicatum Pursh, both native range grasses of the Great Basin, often hybridize in nature. A. trachycaulum, is a tetraploid with 28 chromosomes. It has awnless or awntipped lemmas and small anthers. A. spicatum is a diplois with 14 chromosomes. It is characterized by having long awn that bend at maturity, and anthers which are about four times longer than A. trachycaulum. Artificial hybrids are intermediate between parents in awn and anther lengths and have 21 chromosomes. A natural population in Utah was found consisting of A. trachycaulum, A. spicatum, plants resembling the artificial hybrids, …

Application Of An Electronic Counter In The Evaluation Of Significant Bacteriuria, J. P. Truant, W. A. Brett, K. E. Merckel

Henry Ford Hospital Medical Journal

No abstract provided.

A Study Of The Bacteriological Effectiveness Of Oral Thermometer Procedures In Three Selected Hospitals, Esther Mae Rose

Loma Linda University Electronic Theses, Dissertations & Projects

Because pathogens such as streptococcus and staphylococcus have been transferred in many hospitals, the source of the infections is of concern to doctors, nurses, and other medical personnel. As shown by the review of literature, good techniques are necessary to stop the spread of disease.

It was the purpose of this study to investigate one facet of patient safety by: (1) the culture of thermometers ready for use in three selected hospitals; (2) to determine the effectiveness of the Group, Individual, and Central Supply methods of disinfection currently practiced in the three selected hospitals.

The method for the experiment of …

A Study Of The Effects Of Different Substrates On Growth And Reproduction In Certain Coprophilic Agaricaceae, Raymond C. Brown

Theses and Dissertations

Psilocybe mutans sp. nov. had ceased fruiting consistently in artificial culture several years prior to the initiation of this study. In the couse of this study fruiting bodies were produced on a number of different substrates. All cultures were inoculated by mycelial transfer or by spraying with a suspension of pre-germinated spores. All cultrues were incubated for one to two weeks in a constant-temperature room at 70°F. on a shelf fourteen inches below a fluorescent lighting fixture. Lighting was continuous with an intensity at shelf level of approximately 3,000 foot-candles. Fruiting on any particular substrate was improved if the substrate …