Digital Commons Network^™

Monoamine Oxidase As A Target Enzyme In Pineal And Testicular Tissue: Adrenal Cortical Steroids And Arginine Vasotocin, Randall James Merrill

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The purpose of the research was twofold: (a) To ascertain what effects the adrenal glucocorticoids and catecholamines have on pineal N-acetyltransferase and monoamine oxidase activity, and (b) to observe what effect arginine vasotocin (a pineal polypeptide) has on testicular monoamine oxidase activity.

Rat pineal N-acetyltransferase activity was numerically increased when rats were injected with cortisol. It was increased significantly when the animals were injected with cortisol plus norepinephrine. Adrenal demedullation significantly increased N-acetyltransferase activity in starved rats when compared with intact control animals. Moreover, bovine pineal monoamine oxidase activity was markedly inhibited in a dose-dependent manner when incubated in vitro …

Detection Of Neonatal Calf Diarrhea Virus (Ncdv) And Human Infant Reolike Diarrhea Virus, Martin Wyatt Peterson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The purpose of this study was to develop a diagnostic test and conduct a survey for the neonatal calf diarrhea virus (NCDV) and human infant reolike diarrhea virus. Two immunologic methods were developed in this investigation.

Immune electron microscopy (IEM) and the fluorescent viral precipitin test (FVPT) are the methods used to detect NCDV and the human virus. Both methods are based upon the principle that viral aggregates form when the virus is reacted with anti-NCDV antibody. Aggregates in the IEM method are negatively stained and observed with the use of an electron microscope. Fluorescein labeled antibody is used in …

A Photomicrographic Guide To The Saprolegniaceae Of Illinois, Ronald L. Butler

Masters Theses

There is a paucity of information concerning the occurrence and distribution of the Saprolegniaceae of Illinois. These fungi, commonly called "water molds", are of particular interest as many are potential parasites of fish, crustaceans, and other aquatic plant and animal life. It is of considerable interest to students of the fungi and of practical value to the fisheries biologists and environmentalists to have available a means of identification for these organisms.

This paper provides a descriptive and photomicrographic account of the Saprolegniaceae of Illinois. Included herein are dichotomous keys to the genera of this family as well as generic descriptions …

Biochemical Parameters Of Hereditary Chondrodysplasia In Mice, Trent D. Stephens

Theses and Dissertations

Cartilage from newborn mice homozygous for chondrodysplasia contains normal quantities of protein and chondroitin sulfate. Mutant fetal cartilage incorporates normal amounts of sulfate, glucose, and glucosamine. Overnight rinsing with water removes about 25% of the incorporated sulfur and existing uronic acid from mutant ribs and about 55% from mutant limbs. Control ribs and limbs are affected very little by such a rinse. The diffusability of chondroitin sulfate (represented by incorporated sulfur and uronic acid) may be due to a lack of covalent bond formation between chondroitin sulfate and collagen.

88th Annual Report Of The Nebraska Agricultural Experiment Station

Agricultural Research Division: News and Annual Reports

Contents:
Foreword
Administration and Staff
Nebraska Agricultural Experiment Station
Research Projects
Experiment Station Publications
Publications of the Staff
Report of Income and Expenditures

Agricultural Experiment Station News May 1974

Agricultural Research Division: News and Annual Reports

CONTENTS:
FROM THE DIRECTOR'S DESK
GRANTS AND CONTRACTS
GENERAL NOTES
NEBRASKA AGRICULTURAL EXPERIMENT STATION PUBLICATIONS – April 1974
BULLETINS PRINTED

Nepal Studies Association Newsletter, Issue 5, Nepal Studies Association, John Scholz

Nepal Studies Association Newsletter

No abstract provided.

87th Annual Report Of The Nebraska Agricultural Experiment Station

Agricultural Research Division: News and Annual Reports

Contents:
Foreword
Administration and Staff
Nebraska Agricultural Experiment Station Research Projects
Experiment Station Publications
Publications of the Staff
Report of Income and Expenditures

Effects Of Fungus-Fermented Soybeans On Broiler Growth And Life Cycle Performance Of Coturnix Quail, Cheong Choo Chah

Electronic Theses and Dissertations

Despite the extensive data that have accumulated on the detrimental effect of molds on the growth of farm animals, very little is known as to the beneficial role of fungi in animal feed. Recent studies at South Dakota State University research laboratories revealed, however, that only 164 of 392 strains of Aspergilli were found to be toxic. When the cultures were grown on sterile soybeans or wheat, some of the tested cultures exerted positive effects on growth of chicks and mice. Hence, the investigations reported herein were initiated to obtain further information on those cultures beneficial to chick performance and …

A Taxonomic And Distributional Survey Of The Benthic Blue-Green Algal Flora Of The Skokomish River Estuary, Louis J. Boitano

All Master's Theses

This paper presents a taxonomic and distributional survey of the benthic blue-green algal flora within an estuary system. Physical parameters were considered in the seasonal growth and distribution of algal species. A majority of the observed species occur in all habitats of the estuary and are present throughout the year. Seasonal growth rates and distribution are primarily dependent upon light intensity, temperature, salinity, and substrate condition. The largest extent of growth and distribution occurs in the late spring and summer months at which time the abiotic factors are most favorable for the development of benthic algal communities.

Phenological And Cultural Studies Of Common Dandelion (Taraxacum Officinale Weber), Don Carlisle

Masters Theses & Specialist Projects

Phenological and cultural studies of common dandelion (Taraxacum officinale Weber) were conducted from 1969 to 1971 at Bowling Green, Kentucky. The phenological studies were based upon dandelion plants which were found growing in a Kentucky bluegrass lawn or in open lots. At this location the dandelion flowered throughout the year. Maximum flowering occurred during April and a secondary peak occurred in September and October. The number of flowering stems per square meter was not correlated significantly with precipitation, temperature, or day length. Length of stem was positively correlated with temperature and day length. The plants exhibited diurnal variation in flowering …

Light And Electron Microscopy Investigation Of Developing Wheat Caryopses Infected By Tilletia, Melvin Douglas Grove

Theses and Dissertations

Caryopses of wheat were studied during infection by Tilletia caries and T. controversa. Initially the pathogens penetrated embryos of the host. After the embryo tissue was consumed by the fungi, endosperm tissue was penetrated and consumed. Initial penetration of endosperm tissue was in localized areas. During infection the fungus mycelium spread intercellularly, but occasionally was seen intracellularly. As the host tissue was consumed teliospores were formed. At maturity a host cell layer 4-6 cells thick remained which retained the mature teliospores. Histochemical studies indicated that carbohydrates and lipids of the host disappeared soon after invasion by the pathogens. Nuclei and …

The Role Of Nematodes In A South Dakota Grassland Ecosystem, James D. Smolik

Electronic Theses and Dissertations

In an attempt to elucidate the role of nematodes in a grassland ecosystem nematode taxa, number and biomass data was gathered over a three year period at the Cottonwood International Biological Program Grassland Biome site in western South Dakota. The effects of grazing intensity, sampling date and sampling depth were studied. Nematicide treatment of range grasses in field and greenhouse studies was also used to evaluate the role of nematodes. Biomass data was used to estimate the intake of plant feeding nematodes. Results obtained demonstrate that soil inhabiting nematodes constitute a significant proportion of the consumer biomass at the Cotton[1]wood …

Agricultural Experiment Station News August 1972

Agricultural Research Division: News and Annual Reports

CONTENTS:
FROM THE DIRECTOR'S OFFICE
PERSONNEL ACTIONS
GRANTS AND CONTRACTS
GENERAL NOTES
ALOHA
NEBRASKA AGRICULTURAL EXPERIMENT STATION PUBLICATIONS - July 1972
ABSTRACTS

Nepal Studies Association Newsletter, Issue 2, Nepal Studies Association

Nepal Studies Association Newsletter

No abstract provided.

Quantitative Growth Relationships Of Microcoleus Vaginatus (Gom.) Ex Drouet And Alcaligenes Sp. In A Sucrose-Salts Medium, James Rexford Brownell

Master's Theses

The blue-green alga, Microcoleus vaginatus (Gom.) ex Drouet 1968, and a bacterium, Alcaligenes sp., collected from the same habitat, were cultured together and separately in mineral salts media with and without sucrose. Growth of axenic M. vaginatus was stimulated by sucrose. In association with the bacterium in sucrose-containing media algal growth was suppressed, while in inorganic media it was stimulated. Factors involved in this algal-bacterial system probably included competition for the carbon source, changes in pH, and accumulation of cellular waste and lytic products.

86th Annual Report Of The Nebraska Agricultural Experiment Station

Agricultural Research Division: News and Annual Reports

Contents:
Forward
Administration and Staff
Nebraska Agricultural Experiment Station Research Projects
Experiment Station Publications
Publications of the Staff
Report of Income and Expenditures

Potential Of Pompano Mariculture In Florida, James Anthony Elias

Marine Affairs Theses and Major Papers

Pompano mariculture is not an attempt to bridge the protein gap, but it has been initiated to provide a constant source of this highly desired marine fish. The current market for pompano is small, however, this has been attributed basically to its supply. It appears from the literature that T. carolinus has the most dominant market position since it has an excellent flavor and sports fishermen prefer it due to its fighting ability when hooked on light tackle. It appears from the published history of attempts to raise pompano, by Berry and Iversen, that one of the major problems in …

Analysis Of Asexual Reproduction Dugesia Dorotocephala, Harvey Dee Mecham

Theses and Dissertations

Genetic control of decapitation-induced fission in planaria is mediated by stimulator and inhibitor molecules. Resolution of the two factors was accomplished by filtration chromatography of whole planaria extract. The stimulator (M.W. 2500 to 3500) was purified with ion exchange chromatography and increases the multiple fission rate. The inhibitor (M.W. 20,000 to 50,000) is non-dialyzable, heat labile and is inactivated unless kept frozen. Storage at 4°C results in a loss of inhibitory capacity with a concomitant stimulator increase, suggesting a protein-stimulator complex as the inhibitor. In light of a demonstrated animal intercommunication system, the inhibitor-stimulator complex is hypothesized as part of …

Physiological Regulation Of Cyclic Flowering In Flax, Aurora Salazar Hovland

Electronic Theses and Dissertations

The flowering pattern of flax (Linum usitatissimum, L.) is cyclic in nature with periods of blossoming separated by periods of rest. To study the regulation of flowering cycles, four experiments were conducted. The first two dealt with nutritional aspects of cyclic flowering by imposing environmental stresses which conceivably might regulate flowering pattern nutritionally. The third and fourth experiments dealt with regulation from hormonal systems and included in vitro regulation of bud growth by growth regulators as well as characterization of endogenous hormonal systems by fluorimetry and bioassays. In the first experiment, the effects of light intensity, temperature, nitrogen and defoliation …

The Effects Of Desoxycorticosterone, Hydrocortisone, And Prednisone On Myogenesis In Vitro, Gregory K. Nienaber

Masters Theses

In order to obtain proper muscle development in tissue culture, certain conditions must prevail. There is a delicate balance of factors, and elimination of even one of these factors leads to poor and improper development. Muscle cells grown in the absence of fibroblasts usually fail to differentiate. Therefore, proper development depends upon the presence of two cell types: myoblasts and fibroblasts. Investigators have demonstrated that the fibroblasts are necessary in that they produce collagen which is necessary for proper muscle development. The present study has shown that desoxycorticosterone, hydrocortisone, and prednisone at concentrations of 10, 1, and 0.1 ug/ml of …

Critical Factors In The Management Of Liver Abscesses, Melvin A. Block, Hubert M. Allen

Henry Ford Hospital Medical Journal

Newer diagnostic techniques, including radioisotope liver scans, bacteriologic culture procedures for anaerobes, serologic tests for amebiasis, ultrasonic echograms, and hepatic arteriography, permit more specific identification and localization of hepatic abscesses. Large pyogenic abscesses are of particular Importance to surgeons; are frequently caused by anaerobic organisms; and require early, direct, adequate, and sufficiently prolonged surgical drainage. The clinician should be alert also to recognition and management of the primary lesion responsible for the hepatic abscess, associated abscesses elsewhere, and the possible presence of an underlying liver neoplasm.

Phytoplankton Composition At Lake Drummond In The Dismal Swamp, Virginia, William Howard Poore Jr.

Biological Sciences Theses & Dissertations

Abstract unavailable.

Phytoplankton Periodicity In Shanty Hollow Lake, Warren County, Kentucky, David Hill

Masters Theses & Specialist Projects

A study of the phytoplankton periodicity and its correlation with chemicophysical parameters, diel variation of the plankton, and the cyclomorphotic variation of Ceratium hirundinella was made in Shanty Hollow Lake, Warren County, Kentucky, from October, 1969, to August, 1970.

Phytoplankton samples were collected at zero and six feet with a Kemmerer sampler (31 October through 8 December) and with a ten -liter Birge-Juday plankton trap (23 December through 9 August). Water samples for the chemicophysical determinations were collected with a Kemmerer sampler. The phytoplankton samples were preserved with 5% formalin until they were enumerated. The chemicophysical determinations (pH, conductivity, dissolved …

Factors Affecting Pollination Of Onions In Idaho During 1969, William P. Nye, G. D. Waller, N. D. Waters

All PIRU Publications

No abstract provided.

85th Annual Report Of The Nebraska Agricultural Experiment Station. July 1, 1970 To June 30, 1971

Agricultural Research Division: News and Annual Reports

Contents

Foreword

Administration and Staff

Nebraska Agricultural Experiment Station Research Projects

Experiment Station Publications . .

Publications of the Staff . . . . .

Report of Income and Expenditures

Issued January 1972, 1500

The 85th Annual Report of the Nebraska Agricultural Experiment Station contains a list of all active projects during July 1, 1970 to June 30, 1971, a list of Station publications, staff members, and a financial report. Information derived from the various research projects is published in bulletins, circulars, scientific and trade journals, in the Nebraska Farm, Ranch and Home Quarterly and as mimeographed departmental reports. Station …

Characterization Of Deoxyribonucleic Acid From Actinomycetes, Lynn William Enquist

Theses and Dissertations

Deoxyribonucleic acid (DNA) analyses were used to assess on a molecular level, the relationships among representatives of the genera Streptomyces, Nocardia and Mycobacterium. The methods developed in this study have been used for routine analysis of DNA from a large number of actinomycetes and have given reliable and reproducible data.

DNA isolated from various actinomycetes was characterized by buoyant density determinations in CsCl from which the mole fraction guanine plus cytosine (GC) content was calculated. All the streptomycete DNA preparations studied had buoyant densities in the range of 1.7287 to 1.7312 g cm^-3 which corresponded to GC compositions of …

Algal Growth And Decomposition: Effects On Water Quality, Edward G. Foree, John S. Tapp Jr.

KWRRI Research Reports

The chemical composition of algae grown in batch culture depends mainly on environmental conditions, nutrient availability, presence of predators, cell age, and species. The effects of nutrient availability and cell age on the composition of three unialgal cultures (algae + bacteria) and one hetergeneous culture (algae + bacteria + microscopic animals) were evaluated. The cultures were grown in batch culture under both nutrient-abundant and nutrient deficient conditions and the changes in compositions were observed. Luxurious uptake where nutrients are incorporated into cellular protoplasm at levels greater than those necessary for growth, and super-luxurious uptake, where some nutrients are stored rather …

83rd Annual Report Of The Nebraska Agricultural Experiment Station

Agricultural Research Division: News and Annual Reports

Contents:
Foreword
Administration and Staff
Nebraska Agricultural Experiment Station Research Projects
Experiment Station Publications
Publications of the Staff
Report of Income and Expenditures

Aqueous Microbiology: The Possible Role Of Cell Wall Deficient Bacteria In Uveitis, Philip C. Hessburg, Lioa H. Mattman, Carolyn Barth, Lloyd T. Dutcheshen

Henry Ford Hospital Medical Journal

Samples of aqueous humor were obtained from the anterior chamber of patients with uveitis. Bacterial L-forms, or cell wall deficient forms, were recovered from 11 of 19 eyes with unexplained uveitis. These bacterial variants were cultured on newer, highly specialized media and stained with acridine orange. This stain demonstrates fluorescence of nucleic acids under UV microscopy.