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Scanning Electron Microscopy Of Black Stain On Human Permanent Teeth, J. Theilade, K. M. Pang

Scanning Microscopy

Black stain may develop on the coronal surfaces of human teeth, and this type of stain is common in the Hong Kong Chinese population. The present study was undertaken to ascertain if the deposit conforms in composition to the black stain found elsewhere, and to describe its ultrastructure using the scanning electron microscope. Gram-stained smears were made from black stain on the teeth of 11 adult Hong Kong Chinese and studied microscopically. From another 15 persons extracted teeth exhibiting black stain were obtained and fixed. Two ground sections were made from each tooth, one was stained with toluidine blue, the …

Ultrastructural Analysis Of Enamel Formation During In Vitro Development Using Chemically-Defined Medium, P. Bringas Jr., M. Nakamura, E. Nakamura, J. Evans, H. C. Slavkin

Scanning Microscopy

To test the hypothesis that enamel biomineralization is regulated by sequential expression of an intrinsic genetic program, we designed experiments to determine if a serumless, chemically-defined medium is permissive for position-dependent ameloblast differentiation and subsequent enamel tissue-specific biomineralization in vitro. In the absence of serum or other exogenous growth factors, Swiss Webster strain mouse embryonic (15-and 16-days gestation) mandibular first molar tooth organs (cap stage) developed within 21 days in vitro into well-defined molar tooth organs expressing dentine and enamel biomineralization. Analysis of data obtained from von Kossa histochemistry for calcium salt formation, as well as ultrastructural information obtained …

Clinical Value Of Calcitonin And Carcinoembryonic Antigen Doubling Times In Medullary Thyroid Carcinoma, Charles E. Jackson, Robert A. Norum, Gary B. Talpos, Carolyn S. Feldkamp, Armen H. Tashjian Jr.

Henry Ford Hospital Medical Journal

Doubling times of basal and stimulated calcitonin (CT) levels and of random carcinoembryonic antigen (CEA) levels were compared over time in 11 patients with residual medullary thyroid carcinoma (MTC)—five with the sporadic MTC and six with the hereditary MTC. Four patients with an indolent form of the disease showed little change in CT levels over three to eight years and little change in CEA doubling times (42 to 70+ months). Seven patients showing rapid progression of disease had CT doubling times of three to 11 months and CEA doubling times of two to 12 months (however, one patient showed no …

Quantitation Of Teratogenic Effects Of 5-Fluorouracil Administered To Mice In Vivo Or In Submerged Limb Culture, Theresa A. Sanders

Electronic Theses and Dissertations

This study demonstrates the use of submerged limb culture in teratologic testing. Pregnant mice were treated on day 11 of gestation (E11, plug date = E0) with 10, 20 or 40 mg of 5-fluorouracil (FU) per kg body weight. On E17, treated and untreated fetuses were examined for gross malformations and were fixed in 95% ethanol. Reduction of limb size and digital defects, including ectrodactyly (ED), syndactyly (SD), microdactyly and polydactyly were dose-dependent. In parallel studies, pregnant mice were treated on the morning of E11 and embryos were removed either 7h (E11) or 24h (E12) later for submerged limb culture. …

Effects Of Drought On The Survival Of Rhizobium Leguminosarum Biovar Trifolii And The Nodulation Of Subterranean Clover In An Acid Soil, Carmen Bueno

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Twenty-nine Rhizobium leguminosarum biovar trifolii strains were tested for acidity tolerance in acidified liquid medium. Only 41% of the strains grew at pH 4.1. One acid-tolerant strain, USDA 2160, and one acid-sensitive strain, 162-X-103 from Nitragin Co., were inoculated on seeds of 'Nungarin', 'Seaton Park' and 'Clare' subclover cultivars. The inoculated and pelleted seeds were sown in potted Cluff soil with pH 5.7. Three desiccation levels were imposed by delaying watering for 0, 15 or 30 days. Four gravimetric soil water contents (6.0, 6.6, 10.5 and 12.5%) were maintained under a greenhouse line-source sprinkler system for 7 weeks. The desiccation …

Production Of Acquired Immunodeficiency Syndrome-Associated Retrovirus In Human And Nonhuman Cells Transfected With An Infectious Molecular Clone, Akio Adachi, Howard E. Gendelman, Scott Koenig, Thomas Folks, Ronald Willey, Arnold Rabson, Malcom A. Martin

Nebraska Center for Virology: Faculty Publications

We constructed an infectious molecular clone of acquired immunodeficiency syndrome-associated retrovirus. Upon transfection, this clone directed the production of infectious virus particles in a wide variety of cells in addition to human T4 cells. The progeny, infectious virions, were synthesized in mouse, mink, monkey, and several human non-T cell lines, indicating the absence of any intracellular obstacle to viral RNA or protein production or assembly. During the course of these studies, a human colon carcinoma cell line, exquisitely sensitive to DNA transfection, was identified.

Purification Of Anthrax Toxin Protective Antigen Component And Characterization Of Its Binding Interaction With Bovine Kidney Cells, Daniel Dalton Martin

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Protective antigen component of B. anthracis toxin was produced and purified to the >99% level. Toxin was purified from culture supernatant utilizing concentration and liquid chromatography techniques. Purity was determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

The purified protective antigen retained biological and antigenic activity as evidenced respectively by lethality in Fischer 344 rats when injected in combination with lethal factor, and by positive results on the Ouchterlony double diffussion assay.

Radioiodinated protective antigen was used both in the in vivo and the in vitro experiments.

In vivo distribution of labelled protective antigen was determined in Fischer 344 rats. Assay …

In Situ Growth And Chemical Composition Of The Giant Kelp, Macrocystis Pyrifera: Response To Temporal Changes In Ambient Nutrient Availability, Richard C. Zimmerman, James N. Kremer

OES Faculty Publications

Temporal variations in growth of the giant kelp Macrocystis pyrifera were examined in relation to ambient nutrient availability and chemical composition of mature blades, the primary site of nutrient and carbohydrate storage in M. pyrifera. The effect of nutrient availability on growth was well approximated by a Monod rectangular hyperbola, with growth saturating at ambient nitrate concentra- tions between 1 and 2 FM. M. pyrlfera was unable to generate nutrient reserves that would last beyond 30 d. Nitrogen reserves were stored as free amino acids, and generally constituted about 10 % of total tissue nitrogen. Total nitrogen content was …

Recovery Of Helminths From Prehistoric Feces: The Cultural Ecology Of Ancient Parasitism, Karl J. Reinhard

Karl Reinhard Publications

Several helminth parasites have been recovered from archaeological sites on the Colorado Plateau. These include Ascaris, Trichuris, Enterobius, Moniliformis, and tapeworms in the families Hymenolepidae and Taeniidae. The recovery of these animals demonstrates that cross-temporal: comparison of parasitism between different prehistoric lifestyles is possible. Such comparison might elucidate the effects of changing lifestyle and subsistance pattern on human parasitism.

Desiccated feces recovered from archaelogical deposits were studied. Hunter-gatherer feces were selected from Dust Devil Cave in southeastern Utah. Agriculturalist feces were studied from Antelope House, a cave site in northeastern Arizona, and Salmon Ruins, a Pueblo …

The Annual Meeting Of The Iowa Academy Of Science April 26-27, 1985 [Program, 97th Meeting], Iowa Academy Of Science

Iowa Academy of Science Documents

Program Contents:

Program Summary --- 4
Symposia Programs --- 10
Video Film Festival --- 15
Poster Papers --- 16
Campus Map --- Centerfold
Section Programs --- 18
Agricultural Sciences --- 18
Anthropology --- 21
Biotechnology --- 22
Botany --- 23
Chemical Education --- 28
Chemistry Inorganic --- 29
Chemistry, Organic --- 30
Conservation --- 32
Engineering --- 33
Geology --- 37
Linguistics --- 37
Nursing --- 38
Physics --- 39
Physiology --- 40
Psychology --- 41
Science Teaching --- 43
Zoology --- 44

Survival And Growth Of Adult Human Oligodendrocytes In Culture: Scanning Electron Microscopy, Seung U. Kim, Kookmin M. Kim, Giuseppe Moretto, Doo H. Shin

Scanning Electron Microscopy

The oligodendrocytes in culture serve as an important model for the study of demyelination diseases. We have previously originated a method of isolating human oligodendrocytes. In order to establish their three dimensional morphology, scanning electron microscopy of the cultured oligodendrocytes was performed.

The oligodendrocytes bulk isolated from adult human brain were maintained in culture for more than 2 months. At the various periods in culture, the cells were studied by scanning electron microscopy and immunofluorescence staining using marker antibodies for the identification of oligodendrocytes. The three dimensional organization and the surface morphology of the cultured oligodendrocytes were investigated. They displayed …

Problems Associated With The Preparation Of Cell Suspensions For X-Ray Microanalysis Highlighted By The Comparison Of Results With Those Obtained From Tissue Sections, A. Warley, M. D. Kendall, I. W. Morris

Scanning Electron Microscopy

Fully quantitative X-ray microanalysis of freeze dried frozen sections allowed the concentration of monovalent ions in the nuclei of mouse thymocytes in tissue sections to be compared with those from thymocytes which had been isolated by a simple preparation procedure. Isolated cells showed increased concentrations of Na and Cl. This was further investigated in the rat where the size of the thymus a 11 owed comparison between different isolation media using cells derived from the same animal. Use of autologous serum as the final suspending medium gave reproducible results, whereas with suspension in BSS, or 20% dextran the results were …

Salinity Tolerance In Forage Grasses, Kent Alan Boyles

Masters Theses

The effects of increased osmotic potentials on Reed Canarygrass (Phalaris arundinacea var Vantage), Tall Fescue (Festuca arundinacea var Kentucky 31), Orchardgrass (Dactylis glomerata var Potomac), Sudangrass (Sorghum sudanense var Sorghum x Ludo FS X3-2), Grain Sorghum (Sorghum vulgare var Dekalb 28), Smooth Bromegrass (Bromus inermis var Blair), Timothy (Phleum pratense var Climax), and Winter Barley (Hordeum vulgare var Barsoy) were studied. The greenhouse triplicated experiments were conducted in modified aerated Hoagland's number 2 nutrient solutions for a period of four weeks.

The germination of Winter Barley was not inhibited by osmotic potentials …

Regulation Of Nitrate Reductase Activity In Soybeans, Luke Curtis

Master's Theses

No abstract provided.

Microbial Manganese Reduction By Enrichment Cultures From Coastal Marine Sediments, David J. Burdige, Kenneth H. Nealson

OES Faculty Publications

Manganese reduction was catalyzed by enrichment cultures of anaerobic bacteria obtained from coastal marine sediments. In the absence of oxygen, these enrichment cultures reduced manganates when grown on either lactate, succinate, or acetate in both sulfate-free and sulfate-containing artificial seawaters. Sodium azide as well as oxygen completely inhibited microbial manganese reduction by these enrichment cultures, whereas molybdate had no effect on them. The addition of nitrate to the medium slightly decreased the rate of Mn²⁺ production by these enrichment cultures. These findings are consistent with the hypothesis that the manganese-reducing organisms in these enrichment cultures use manganates as terminal …

Effect Of Physiological Age On Radiation Resistance Of Some Bacteria That Are Highly Radiation Resistant, Lewis Keller, R. Burt Maxcy

Department of Food Science and Technology: Faculty Publications

Physiological age-dependent variation in radiation resistance was studied for three bacteria that are highly radiation resistant: Micrococcus radiodurans, Micrococcus sp. isolate C-3, and Moraxella sp. isolate 4. Stationary-phase cultures of M. radiodurans and isolate C-3 were much more resistant to gamma radiation than were log-phase cultures. This pattern of relative resistance was reversed for isolate 4. Resistance of isolate 4 to UV light was also greater during log phase, although heat resistance and NaCl tolerance after heat stress were greater during stationary phase. Radiation-induced injury of isolate 4 compared with injury of Escherichia coli B suggested that the injury process, …

The Isolation And Identification Of Legionella Species From Environmental Sites In Rowan County, Kentucky, Sabo E. Yakubu

Morehead State Theses and Dissertations

A thesis presented to the faculty of the School of Sciences and Mathematics at Morehead State University in partial fulfillment of the requirements for the Degree of Master of Science by Sabo E. Yakubu on December 17, 1983.

Infection Of Oriental Mustard By Nematospora: A Fluorescence And Scanning Electron Microscope Study, Richard A. Holley, Beverley E. Phipps-Todd, Suk H. Yiu

Food Structure

Fluorescence light microscopy and scanning electron microscopy were used to study penetration by the yeast Nematospora caryli through the seed coat and into the embryonic tissues of oriental mustard seed (Brasslca iuncea).

Infection of the seed was associated with its physical injury; however, it was evident that the yeast was capable of successfully invading healthy plant cells. The pathological process was followed in parallel using both the above types of microscopy. Foci of yeast infection on the seed coat outer surface were characterized by swelling of the infected epidermal cells. Nematospora hyphae were seen in the lumina of the seed …

Books Received And Book Notices, The Jonathan Nāpela Center For Hawaiian And Pacific Islands Studies

Pacific Studies

Book Listing for the Vol 6, No 1 Fall Edition of the Pacific Studies Journal

Meetings & Announcements

International Journal for the Study of Animal Problems

The first international meeting on the Human/Companion Animal Bond was held at the University of Pennsylvania during October 5-7, 1981. The meeting brought together persons concerned about animal welfare and a broad spectrum of healthcare professionals. Two symposia were held in Europe in late 1981 (The Netherlands and Sweden) on the LD50 test. In October 1981, a symposium was organized in Switzerland on using animals in research and testing. At the end of 1981, the National Society for Medical Research organized an “adjunct” methods seminar. Finally, the Scientists Center for Animal Welfare organized a conference on the regulation of animal …

Legislation And Regulation

International Journal for the Study of Animal Problems

The idea of new federal regulation on the care and use of animals in research is no longer novel; bills that would control and refocus the conduct of animal experimentation in the U.S. have been pending since the last session of Congress. Last autumn, however, a new phase in the process began. On 13-14 October 1981, the House Subcommittee on Science, Research, and Technology held information-gathering public hearings to evaluate existing bills and possibly formulate its own legislation.

Strategies For Oncogenesis By Avian Leukosis Viruses, Benjamin G. Neel

Student Theses and Dissertations

The mechanism of oncogenesis by avian leukosis viruses (ALVs) was examined. ALVs are slowly transforming RNA tumor viruses that do not transform cells in tissue culture and that cause neoplastic disease only after a latent period of 4-12 months. ALVs generally induce widely disseminating B cell lymphomas originating in the bursae of Fabricius of infected birds. Occasionally, they cause other neoplasms such as sarcomas, carcinomas and nephroblastomas, as well as a variety of nonneoplastic diseases. Unlike rapidly transforming RNA tumor viruses, which appear to cause neoplastic transformation as a result of high expression (or inappropriate expression) of a "transforming gene" …

Polyculture Of Giant Malaysian Prawns (Macrobrachium Rosenbergii) And Fathead Minnows (Pimephales Promelas), Tommie Crawford, Mike Freeze

Journal of the Arkansas Academy of Science

No abstract provided.

Effect Of Toxaphene On Collagen Synthesis In Fish Tissue: Organ Culture Studies And Prolyl Hydroxylase Activity Assay, Charles Franklin Luke

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Toxaphene is reported to cause defects in the collagen of fish. Chronic exposure to toxhaphene weakens the backbone of fish by decreasing the amount of collagen and usually increasing the amount of calcium in the bone which results in a more brittle and fragile bone.

We investigated the possible direct action of toxaphene on collagen synthesis by exposing vertebral and swim bladder organ cultures obtained from unexposed rainbow trout (Salmo gairdneri) fingerlings to the same lot of toxaphene found to cause this defect in vivio. Collagen produced by these organ cultures was measured by: (1) total ^{3 …}

Anti-Neoplastic Actions In Vivo And In Vitro By Non-Toxic Natural Products From Chara Globularis, A Multicellular Green Alga, Abdessalam Sherif

Theses Digitization Project

No abstract provided.

Nepal Studies Association Bulletin, No. 21, Nepal Studies Association, Donald A. Messerschmidt

Nepal Studies Association Newsletter

No abstract provided.

The In Vitro And In Vivo Effects Of Sm-1213 Drug Therapy On Pathogenesis And Host Defense In Experimentally Induced Candidiasis, Christine Joy Morrison

Master's Theses

No abstract provided.

In Vitro Experiments On Totipotency Of Dacus Carota, Karl L. Ruser

Journal of the Minnesota Academy of Science

The intent of this project was to induce the totipotency of carrot cells in tissue culture. The information gathered would be applied to work with woody plants. Differentiated cells removed from the root were cultured in vitro using a modified White's medium. Callus was initiated and successfully sub cultured. Roots and the production of chlorophyll have been initiated in the cultures. Because some specialized equipment and facilities for tissue culture were not available at the beginning of the project, it took considerable time to gather and improvise them. A roller tube culture mixer was constructed, using the mechanism from a …

Comparison Of Tolerance To High Moisture Conditions Of The Soil Among Crop Plants: Studies On The Comparative Plant Nutrition [English Translation], Toshiaki Tadano, Kiyokazu Kirimoto, Isao Aoyama, Arika Tanaka

Publications

Tolerance to high moisture conditions in the soil was compared for eighteen crop species, and factors causing differences among species were studied: 1) When the moisture content of a soil was kept high, the soil air contained 10.5 - 17.5% of O2 and 5.0 - 9.8% of CO2, and the soil solution was not very high in Fe, Mn or NO2-N. 2) Cruciferae, Solanaceae, sugar beet, field bean, pea, cucumber and Chrysanthemum coronarium were very susceptible to this condition, but maize, soybean, and onion were tolerant. Rice grew better under this condition than under ordinary upland conditions. 3) Tolerance to …

Siderocapsa Major-- Fact Or Artifact?, Ray W. Swanson

Masters Theses

The validity of the genus Siderocapsa, a group of unicellular iron-depositing bacteria, has been a subject of debate for many years. Lack of photomicroscopic and cultural studies has kept this group in taxonomic obscurity. Bacteria were collected from the Campus pond, Eastern Illinois University, Charleston, Illinois, by a submerged slide/coverslip technique. These bacteria were identified as Siderocapsa major according to the description of Molisch (1909). Scanning electron microscopy of Siderocapsa major microcolonies has shown cells (0.5 by 1.15 μm), in a clear central well (2-6 μm), surrounded by an amorphous material which accumulates with the age of the colony. …