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Trapping Small Hive Beetles, Aethina Tumida Murray, Inside Honey Bee Colonies, Maxcy Nolan Iv

All Theses

The small hive beetle, Aethina tumida Murray, (Coleoptera: Nitidulidae) is an economically important honey bee pest, particularly in the southeastern United States. One method of controlling this pest is the use of an in-hive trap. Many different attractants have been developed and are used in such traps. Part one of my thesis involved testing a new attractant, a pollen substitute inoculated with the yeast Kodamaea ohmeri (NRRL Y-30722). Thirty-two test colonies were established with 0.9-kg (2-lb) packages of bees with queens. Eight colonies were placed in each apiary, and colonies received one of three treatments: 1) yeast-based attractant 2) apple …

Limnological Assistance For The Lake Mead National Recreation Area In Meeting The Challenge Of The Water 2025 Initiative: Quarterly Report, Period Ending September 30, 2008, Margaret N. Rees

Limnological Studies

Project 1: Technical input has been provided at eight advisory team meetings attended this quarter. Broad, overarching “Issues of Concern” developed for the Boulder Basin Adaptive Management Plan are currently under review by Drs. David Wong and Shawn Gerstenberger. A Selenium Management Plan is also under review.

Project 2: Data collected by the Southern Nevada Water Authority has been synthesized to summarize bacteria counts in high-use areas of Lakes Mead and Mohave. This information will be documented and interpreted for management use in a formal report in the upcoming quarter.

Project 3: A draft document titled “Suggested Standard Methods for …

Introduction To The Limnology And Oceanography Special Issue On Autonomous And Lagrangian Platforms And Sensors (Alps), Mark A. Moline, T. D. Dickey, E. C. Itsweire, M. J. Perry

Biological Sciences

This issue is devoted to recent developments of autonomous and Lagrangian platforms and sensors (ALPS) and their uses for solving a broad range of interdisciplinary aquatic problems. The collective papers treat a variety of problems that generally require measurements spanning a continuum of temporal and spatial scales. ALPS platforms in this issue include surface drifters, profiling and other types of subsurface floats, gliders, unmanned boats, autonomous underwater vehicles, and instrumented animals. ALPS platforms can provide access in difficult environments (e.g., under ice and in high-sea states). They are also important for emerging networked ocean and lake observing systems that require …

Estimates For Worldwide Laboratory Animal Use In 2005, Katy Taylor, Nicky Gordon, Gill Langley, Wendy Higgins

Laboratory Experiments Collection

Animal experimentation continues to generate public and political concern worldwide. Relatively few countries collate and publish animal use statistics, yet this is a first and essential step toward public accountability and an informed debate, as well as being important for effective policy-making and regulation. The implementation of the Three Rs (replacement, reduction and refinement of animal experiments) should be expected to result in a decline in animal use, but without regular, accurate statistics, this cannot be monitored. Recent estimates of worldwide annual laboratory animal use are imprecise and unsubstantiated, ranging from 28–100 million. We collated data for 37 countries that …

The Beginning Of The End For Chimpanzee Experiments?, Andrew Knight

Experimentation Collection

The advanced sensory, psychological and social abilities of chimpanzees confer upon them a profound ability to suffer when born into unnatural captive environments, or captured from the wild – as many older research chimpanzees once were – and when subsequently subjected to confinement, social disruption, and involuntary participation in potentially harmful biomedical research. Justifications for such research depend primarily on the important contributions advocates claim it has made toward medical advancements. However, a recent large-scale systematic review indicates that invasive chimpanzee experiments rarely provide benefits in excess of their profound animal welfare, bioethical and financial costs. The approval of large …

Microbial Colonization Of Deep-Sea Hydrothermal Vent Chimneys, Antoine Pagé

Dissertations and Theses

Deep-sea hydrothermal vent chimneys harbor some of the most extreme environments supporting life on Earth. In the absence of sunlight, the microbial communities that colonize these deposits are based on energy harvested by chemolithoautotrophs exploiting the chemical disequilibria created as hydrothermal fluids mix with seawater. Although controversial, several models consider deep-sea vent chimneys as likely habitats for primitive ecosystems that developed during the Archean.

The goal of this study was to describe spatial and temporal patterns of microbial colonization in deep-sea vent chimneys emitting high temperature (>300°C) fluids. Microbial community composition and structure were evaluated through analyses of 16S …

Analysis Of The Cyc1 Promoter In Candida Albicans, Tomica Blocker

McCabe Thesis Collection

Scientists are considering two factors that may be important in the pathogenesis of C. albicans', it's capability to morph from yeast to hyphal phase, and its differences in colony morphology, cell shape, cell surface and cell permeability. (5) Unfortunately, unlike the bacteria, yeasts do not yet have proven virulence factors. However, science is suggesting that some of the major factors which contribute to the virulence of Candida are its ability to form hyphae, its ability to resist phagocytosis, its ability to adhere to epithelial cell surfaces, its ability to grow well at 37 degrees Celsius, and its ability to secrete …

A Behavioral Assay To Quantify Retinal Degeneration In X. Laevis, Jacob Fleming

Renée Crown University Honors Thesis Projects - All

Currently, 3.5 million Americans over the age of 40 are blind or visually impaired. Cases of visual impairment can be attributed to a number of causes. Retinitis pigmentosa, glaucoma, and macular degeneration all involve some form of damage to the retina. While medical advances continue to aid those with vision loss, there is currently no cure for visual impairment due to cell death. In the age of stem cells however, there is hope for those with retinal damage.

Seven eye field transcription factors play specific roles in eye development. When injected into developing Xenopus laevis, these transcription factors induce …

Influential Parasites: Importance Of Haemosporidian Parasite Infection In The White-Throated Sparrow, Amanda P. Jamison

All-Inclusive List of Electronic Theses and Dissertations

The study of parasite-mediated selection, as originally postulated by Hamilton and Zuk ( 1982) in birds is an area of intense study as it allows us to test the significance of the relationship between host and parasite. Recent developments of resistance to treatments in humans for parasite induced disease, creates a place for this important research and emphasizes the need to find the connections and interactions in this relationship between ecology and infectious disease. The development of new molecular techniques has made the study of parasitism in birds and mammals easier and provides an efficient sensitive . methodology to detect …

Systems Approach Identifies An Organic Nitrogen-Responsive Gene Network That Is Regulated By The Master Clock Control Gene Cca1, Rodrigo A. Gutierrez, Trevor L. Stokes, Karen Thum, Xiaodong Xu, Mariana Obertello, Manpreet S. Katari, Milos Tanurdzic, Alexis Dean, Damion C. Nero, C Robertson Mcclung, Gloria M. Coruzzi

Dartmouth Scholarship

Understanding how nutrients affect gene expression will help us to understand the mechanisms controlling plant growth and development as a function of nutrient availability. Nitrate has been shown to serve as a signal for the control of gene expression in Arabidopsis. There is also evidence, on a gene-by-gene basis, that downstream products of nitrogen (N) assimilation such as glutamate (Glu) or glutamine (Gln) might serve as signals of organic N status that in turn regulate gene expression. To identify genome-wide responses to such organic N signals, Arabidopsis seedlings were transiently treated with ammonium nitrate in the presence or absence of …

Conditional Protein Alleles And Regulation Of Bacterial Transcription: A Thesis In Two Acts, Edmund Ching Schwartz

Student Theses and Dissertations

The use of small molecule modulators of protein function (drugs) has gained in popularity due to the speed and precision with which they can work. Unfortunately, a small molecule with the required specificity and potency is not always available. We have developed two methods to generate small molecule sensitive protein alleles without the need to screen for a new drug. In the first, which we term conditional protein splicing (CPS), an intein is split into two inactive fragments which are activated by heterodimerization. By fragmenting the target protein and fusing the pieces to this split intein, we were able to …

Biotinyl-Methyl 4-(Amidomethyl)Benzoate Is A Competitive Inhibitor Of Human Biotinidase, Keyna Kobza, Kittichai Chaiseeda, Gautam Sarath, James M. Takacs, Janos Zempleni

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Posttranslational modification of histones by biotinylation can be catalyzed by both biotinidase (BTD) and holocarboxylase synthetase. Biotinylation of histones is an important epigenetic mechanism to regulate gene expression, DNA repair, and chromatin remodeling. The role of BTD in histone biotinylation is somewhat ambiguous, given that BTD also catalyzes removal of the biotin tag from histones. Here, we sought to develop BTD inhibitors for future studies of the role of BTD in altering chromatin structure. We adopted an existing colorimetric BTD assay for use in a novel 96-well plate format to permit high-throughput screening of potential inhibitors. Biotin analogs were chemically …

History Of The Department Of Biochemistry And Molecular Biology, Gene A. Homandberg

UND Departmental Histories

This departmental history was written on the occasion of the UND Quasquicentennial in 2008.

Interagency Science And Research Strategy, Limnological Assistance For Lake Mead National Recreation Area

Limnological Studies

Helping improve operational efficiency, enhance land stewardship, resource protection, and conservation on the shared public land.

Dms Air/Sea Flux And Gas Transfer Coefficients From The North Atlantic Summertime Coccolithophore Bloom, Christa Marandino, Warren J. De Bruyn, Scott Miller, Eric S. Saltzman

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Dimethylsulfide (DMS) atmospheric and oceanic concentrations and eddy covariance air/sea fluxes were measured over the N. Atlantic Ocean during July 2007 from Iceland to Woods Hole, MA, USA. Seawater DMS levels north of 55 degrees N ranged from 3 to 17 nM, with variability related to the satellite-derived distributions of coccoliths and to a lesser extent, chlorophyll. For the most intense bloom region southwest of Iceland, DMS air/sea fluxes were as high as 300 mu mol m(-2) d(-1), larger than current model estimates. The observations imply that gas exchange coefficients in this region are significantly greater than those estimated using …

Monitoring Programs To Assess Reintroduction Efforts: A Critical Component In Recovery, Erin Muths, Victoria J. Dreitz

Wildlife Biology Faculty Publications

Reintroduction is a powerful tool in our conservation toolbox. However, the necessary follow–up, i.e. long–term monitoring, is not commonplace and if instituted may lack rigor. We contend that valid monitoring is possible, even with sparse data. We present a means to monitor based on demographic data and a projection model using the Wyoming toad (Bufo baxteri) as an example. Using an iterative process, existing data is built upon gradually such that demographic estimates and subsequent inferences increase in reliability. Reintroduction and defensible monitoring may become increasingly relevant as the outlook for amphibians, especially in tropical regions, continues to …

A History Of The Biology Department, Omer R. Larson

UND Departmental Histories

This departmental history was written on the occasion of the UND Quasquicentennial in 2008.

Giardia Lamblia: Genomic And Molecular Analyses Of Flippase, Diana Leticia Villazana-Kretzer

Open Access Theses & Dissertations

Giardia lamblia, is an intestinal protozoan parasite responsible as a major cause of water-borne intestinal infection in humans (giardiasis). It has been proposed earlier that Giardia has limited lipid synThesis ability and therefore relies on preformed lipid molecules from outside sources. It has also been proposed that lipids are taken up through passive and active transport, and giardial flippasse play a significant role in this process. This study describes the identification and characterization of a putative homologue of flippase gene from Giardia. Flippase is an enzyme that functions as a trans-bilayer transport system facilitating the flip flop diffusion of phospholipids …

Evolutionary And Structural Analyses Of Alpha-Papillomavirus Capsid Proteins Yields Novel Insights Into L2 Structure And Interaction With L1, John Lowe, Debasis Panda, Suzanne Rose, Ty Jensen, Willie Hughes, For Yue Tso, Peter C. Angeletti

Nebraska Center for Virology: Faculty Publications

Background: PVs (PV) are small, non-enveloped, double-stranded DNA viruses that have been identified as the primary etiological agent for cervical cancer and their potential for malignant transformation in mucosal tissue has a large impact on public health. The PV family Papillomaviridae is organized into multiple genus based on sequential parsimony, host range, tissue tropism, and histology. We focused this analysis on the late gene products, major (L1) and minor (L2) capsid proteins from the family Papillomaviridae genus Alpha-papillomavirus. Alpha-PVs preferentially infect oral and anogenital mucosa of humans and primates with varied risk of oncogenic transformation. Development of evolutionary associations between …

Pyridinium Bisretinoids: Synthesis And Photoactivated Cytotoxicity, Junping Gao

Theses and Dissertations

This thesis discusses pyridinium bisretinoid compounds (PBRs), which were prepared for two purposes: 1) to use them as standards for detection of novel fluorophores in human RPE cells, which may be involved in age-related macular degeneration (AMD), and 2) to use them in the development of a targeted and triggered drug delivery system for cancer therapy. We prepared a selection of PBRs using a one-pot biomimetic method; synthesis, mechanisms for formation, and characterization of these compounds is described. We also explored the photoreactivity of three novel PBR compounds and found that these PBRs form oxidation products under blue-light irradiation. The …

A Non-Circadian Role For Clock-Genes In Sleep Homeostasis: A Strain Comparison, Paul Franken, Ryan Thomason, H. Craig Heller, Bruce F. O'Hara

Biology Faculty Publications

BACKGROUND: We have previously reported that the expression of circadian clock-genes increases in the cerebral cortex after sleep deprivation (SD) and that the sleep rebound following SD is attenuated in mice deficient for one or more clock-genes. We hypothesized that besides generating circadian rhythms, clock-genes also play a role in the homeostatic regulation of sleep. Here we follow the time course of the forebrain changes in the expression of the clock-genes period (per)-1, per2, and of the clock-controlled gene albumin D-binding protein (dbp) during a 6 h SD and subsequent recovery sleep in three inbred strains of mice for which …

Gene Synthesis, Cloning, Expression, Purification And Biophysical Characterization Of The C2 Domain Of Human Tensin, Kiran Sukumar Gajula

Doctoral Dissertations

Tensin is a large "docking" protein found in the adhesive junctions of animal cells and recruited early in the development of cell-substrate contacts. There it binds to the cytoplasmic domain of integrin β1 and caps the barbed ends of filamentous actin. This forms a rational basis for its implication in a direct role in the mechanics of membrane-cytoskeleton interactions. Tensin provides a physical link between the actin cytoskeleton, integrins, and other proteins at the cell-substrate contacts. Its overall biochemical properties are a function of its domain composition and architecture, i.e., the domains that are present and their relative positions in …

Evaluating The Medicinal Properties Of Mandravasarotra (Cinnamosa Sida) Through Chemical And Pharmacological Screening, Henry J. Bowden Jr.

Independent Study Project (ISP) Collection

I worked for four weeks at the University of Antananarivo (Ankatso), spending two weeks each in the chemistry and pharmacology laboratories. There I ran a total of eight experiments or “screens” with an alcoholic extract of the medicinal plant Mandravasarotra. This plant is found mainly in northwestern Madagascar, and is often used to stimulate contractions for women having difficulty giving birth. The plant tested positive for three secondary metabolites: tannins, steroids and flavanoids. In the pharmacological tests, the plant exhibited anti-inflammatory properties, and high toxicity in doses exceeding 10ml/kg. Together, conjectures may be made between what is known of the …

A Simple Array Platform For Microrna Analysis And Its Application In Mouse Tissues, Xiaoqing Tang, Jozsef Gal, Xun Zhuang, Wang-Xia Wang, Haining Zhu, Guiliang Tang

Plant and Soil Sciences Faculty Publications

MicroRNAs (miRNAs) are a novel class of small noncoding RNAs that regulate gene expression at the post-transcriptional level and play a critical role in many important biological processes. Most miRNAs are conserved between humans and mice, which makes it possible to analyze their expressions with a set of selected array probes. Here, we report a simple array platform that can detect 553 nonredundant miRNAs encompassing the entire set of miRNAs for humans and mice. The platform features carefully selected and designed probes with optimized hybridization parameters. Potential cross-reaction between mature miRNAs and their precursors was investigated. The array platform was …

Benthic Mapping For Habitat Classification In The Peconic Estuary: Phase I Groundtruth Studies, Robert M. Cerrato, Nicole P. Maher

School of Marine & Atmospheric Sciences Faculty Publications

Benthic habitat maps of the estuary seafloor will increase our knowledge of range and variability in benthic habitats, will assist managers in their efforts to protect and/or restore commercially and recreationally important finfish and shellfish, will link land usage (e.g. developed vs. undeveloped areas) and water quality data to benthic habitat quality, and will make it possible to utilize faunal data as a long-term indicator of the overall “health” of the estuary. We are developing benthic habitat maps by combining high-resolution remote sensing techniques with detailed study of the physical and faunal characteristics at point locations in different seafloor environments. …

Animal Minds, Cognitive Ethology, And Ethics, Colin Allen, Marc Bekoff

Sentience Collection

Our goal in this paper is to provide enough of an account of the origins of cognitive ethology and the controversy surrounding it to help ethicists to gauge for themselves how to balance skepticism and credulity about animal minds when communicating with scientists. We believe that ethicists’ arguments would benefit from better understanding of the historical roots of ongoing controversies. It is not appropriate to treat some widely reported results in animal cognition as if their interpretations are a matter of scientific consensus. It is especially important to understand why loose references to ‘‘cognitive ethology’’ by philosophers can signal ignorance …

Managing The Recreational Catch Of Demersal Scalefish On The West Coast. Future Management Scenarios For Community Consideration, Jon Ford (Minister For Fisheries; The Kimberley, Pilbara And Gascoyne)

Fisheries Management Papers

This paper discusses the major issues around controlling the total recreational catch. It also considers some of the tools available to manage the catch to specific target levels. The sustainability of fish species must be a key driver. However, the impacts on the social amenity of recreational fishing must also be taken into account. Any particular tool, if used on its own and to its full extent, may be rather “blunt” in its application and its impacts unacceptable to large parts of the community. The most effective and acceptable management package will probably be a mix of tools, supported by …

Fully Codon-Optimized Luciferase Uncovers Novel Temperature Characteristics Of The Neurospora Clock, Van D. Gooch, Arun Mehra, Luis F. Larrondo, Julie Fox, Melissa Touroutoutoudis, Jennifer J. Loros, Jay C. Dunlap

Dartmouth Scholarship

We report the complete reconstruction of the firefly luciferase gene, fully codon optimized for expression in Neurospora crassa. This reporter enhances light output by approximately 4 log orders over that with previously available versions, now producing light that is visible to the naked eye and sufficient for monitoring the activities of many poorly expressed genes. Time lapse photography of strains growing in race tubes, in which the frq or eas/ccg-2 promoter is used to drive luciferase, shows the highest levels of luciferase activity near the growth front and newly formed conidial bands. Further, we have established a sorbose medium colony …

A Genome-Scale Approach To Phylogeny Of Ray-Finned Fish (Actinopterygii) And Molecular Systematics Of Clupeiformes, Chenhong Li

School of Biological Sciences: Dissertations, Theses, and Student Research

The current trends in molecular phylogenetics are towards assembling large data matrices from many independent loci and employing realistic probabilistic models. Large genome-scale data sets shall reduce the sampling error, whereas complex models accommodating heterogeneity among sites and along the phylogenetic tree can decrease systematic errors. The theme of this dissertation project is using both bioinformatic and experimental approaches to develop genome-scale nuclear gene markers and applying them in studies of phylogeny of ray-finned fish (Actinopterygii) and systematics of clupeiforms. Bioinformatic tools and computer programs were developed to search for conserved single-copy nuclear genes with long exons. By comparing within …

Slides: A Case Study Of The Roan Plateau Area, Mary Viviano

The Future of Natural Resources Law and Policy (Summer Conference, June 6-8)

Presenter: Mary Viviano, EnCana Oil & Gas (USA) Inc.

14 slides