Digital Commons Network^™

Colour, Light, And Materiality: Biophilic Interior Design Presence In Research And Practice, Beth Mcgee, Nam-Kyu Park

Interiority

The biophilic design hypothesis uses nature-based environmental design for optimising people’s health and well-being. Stephen Kellert in 2008 developed a list of biophilic attributes that was further refined in the Biophilic Interior Design Matrix (BID-M) to specifically support the interior application of biophilic design for health and well-being. The present study further investigates biophilic interior design using the BID-M language and the key interior design components colour, light, and materiality. The first part of the study reviewed four decades of literature related to biophilia and colour, light, and materiality to investigate a total of 19 publications. The second part of …

Essential Oils From Artemisia Herba Alba Asso., Maticaria Recutita L., And Dittrichia Viscosa L. (Asteraceae): A Promising Source Of Eco-Friendly Agents To Control Callosobruchus Maculatus Fab. Warehouse Pest, Allali Aimad, Mohammed Bourhia, Hadin Hana, Rezouki Sanae, Ahmad Mohammad Salamatullah, Walid Soufan, Hail Z. Rihan, Lahcen Ouahmane, El Abdali Youness, Eloutassi Noureddine, Fadli Mohamed

School of Biological and Marine Sciences

Callosobruchus maculatus (Fab.) (C. maculatus) is one of the major pests of legume seeds in storage causing significant damage, leading to food insecurity and low income for farmers. This work was planned to develop eco-friendly agents from essential oils of Artemisia herba alba Asso. (AEO), Maticaria Recutita L. (MEO), and Dittrichia Viscosa L. (DEO) to control C. maculatus. To achieve this goal, essential oils (EOs) were extracted by hydro-distillation using Clevenger apparatus before being characterized by GC-MS. EOs were used for testing purposes using three different tests, namely, inhalation toxicity, contact toxicity, and repellency tests. GC-MS analysis of EOs showed …

Nanostring Digital Molecular Profiling Of Protein And Microrna In Rhabdomyosarcoma., Atif Ahmed, Midhat S. Farooqi, Sahibu Sultan M Habeebu, Elizabeth Gonzalez, Terrie Flatt, Ashley L. Wilson, Frederic G. Barr

Manuscripts, Articles, Book Chapters and Other Papers

Purpose: Rhabdomyosarcoma (RMS) exhibits a complex prognostic algorithm based on histologic, biologic and clinical parameters. The embryonal (ERMS) and spindle cell-sclerosing RMS (SRMS) histologic subtypes warrant further studies due to their heterogenous genetic background and variable clinical behavior. NanoString digital profiling methods have been previously highlighted as robust novel methods to detect protein and microRNA expression in several cancers but not in RMS.

Methods/patients: To identify prognostic biomarkers, we categorized 12 ERMS and SRMS tumor cases into adverse (n = 5) or favorable (n = 7) prognosis groups and analyzed their signaling pathways and microRNA profiles. The digital …

Mosquito Edge: An Edge-Intelligent Real-Time Mosquito Threat Prediction Using An Iot-Enabled Hardware System, Shyam Polineni, Om Shastri, Avi Bagchi, Govind Gnanakumar, Sujay Rasamsetti, Prabha Sundaravadivel

Electrical Engineering Faculty Publications and Presentations

Species distribution models (SDMs) that use climate variables to make binary predictions are effective tools for niche prediction in current and future climate scenarios. In this study, a Hutchinson hypervolume is defined with temperature, humidity, air pressure, precipitation, and cloud cover climate vectors collected from the National Oceanic and Atmospheric Administration (NOAA) that were matched to mosquito presence and absence points extracted from NASA's citizen science platform called GLOBE Observer and the National Ecological Observatory Network. An 86% accurate Random Forest model that operates on binary classification was created to predict mosquito threat. Given a location and date input, the …

Clinical Performance Of Methylation As A Biomarker For Cervical Carcinoma In Situ And Cancer Diagnosis: A Worldwide Study, Cristiana Banila, Attila T. Lorincz, Dorota Scibior-Bentkowska, Gary M. Clifford, Birhanu Kumbi, Dereje Beyene, Cosette M. Wheeler, Kate Cuschieri, Jack Cuzick, Belinda Nedjai

Pathology Research and Scholarship

The shift towards primary human papillomavirus (HPV)-based screening has necessitated the search for a secondary triage test that provides sufficient sensitivity to detect high-grade cervical intraepithelial neoplasia (CIN) and cancer, but also brings an improved specificity to avoid unnecessary clinical work and colposcopy referrals. We evaluated the performance of the previously described DNA-methylation test (S5) in detecting CIN3 and cancers from diverse geographic settings in high-, medium- and low-income countries, using the cut-off of 0.80 and exploratory cut-offs of 2.62 and 3.70. Assays were performed using exfoliated cervical specimens (n = 808) and formalin-fixed biopsies (n = 166) from women …

Fbxw7-Mediated Erk3 Degradation Regulates The Proliferation Of Lung Cancer Cells, Hyun-Jung An, Cheol-Jung Lee, Ga-Eun Lee, Youngwon Choi, Dohyun Jeung, Weidong Chen, Hye Suk Lee, Han Chang Kang, Joo Young Lee, Dae Joon Kim

School of Medicine Publications

Extracellular signal-regulated kinase 3 (ERK3) is an atypical member of the mitogen-activated protein kinase (MAPK) family, members of which play essential roles in diverse cellular processes during carcinogenesis, including cell proliferation, differentiation, migration, and invasion. Unlike other MAPKs, ERK3 is an unstable protein with a short half-life. Although deubiquitination of ERK3 has been suggested to regulate the activity, its ubiquitination has not been described in the literature. Here, we report that FBXW7 (F-box and WD repeat domain-containing 7) acts as a ubiquitination E3 ligase for ERK3. Mammalian two-hybrid assay and immunoprecipitation results demonstrated that ERK3 is a novel binding partner …

Bis-Amino Acid Based Compound And Use Thereof, George Gokel, Joseph W. Meisel, Mohit B. Patel

UMSL Patents

Compositions comprising bis(tryptophan) derivatives are provided that act as antimicrobials. Also provided are methods for reversing antibiotic resistance in a bacterium, or recovering or enhancing antimicrobial activity of an antibiotic against a variety of microbes, by co-administration with a bis(tryptophan) derivative.

Phytochemical Investigation Of Egyptian Riverhemp: A Potential Source Of Antileukemic Metabolites, Shimaa M. Abdelgawad, Mona H. Hetta, Mohamed A. Ibrahim, Premalatha Balachandran

Faculty and Student Publications

As part of our research group's continuous efforts to find alternative treatments for cancer, the aqueous ethanol extract of Sesbania sesban L. Merr. (SS, Egyptian riverhemp) demonstrated an antileukemic activity against K562 cell line. Bioguided fractionation of SS leaves hydroethanolic extract resulted in the isolation of one new compound (33) named as hederatriol 3-O-β-D-glucuronic acid methyl ester as well as 34 known compounds. Seven compounds ((34), (22), (20), (24), (21), (19), and (35)) showed high antiproliferative effects (IC50 = 22.3, 30.8, 31.3, 33.7, 36.6, 37.5, and 41.5 μM, respectively), while four compounds ((32), (5), (29), and (1)) showed milder activities …

Stylosanthes Scabra - Environmental Weed Risk Assessment 2022, Department Of Primary Industries And Regional Development, Western Australia

Environmental Weed Risk Assessments

Introducing new plants to an area may have both positive and negative effects on the environment, economy and community. To minimise the negative environmental impact of introducing new agricultural species, DPIRD conducts a risk assessment procedure based on widely accepted scientific standards. This report assesses Shrubby stylo (Stylosanthes scabra). Shrubby stylo (Stylosanthes scabra) is a shrubby, perennial legume, erect to suberect, with strong, woody stems, usually densely hairy and viscid, making them sticky under hot, dry conditions. It is a widespread and variable species, originating from South America and the Caribbean (Sousa Costa and Ferreira 1984) with two ecotypes identified …

Generalization Of The Ratio Of Minimized Kullback- Leibler Divergence Discrimination Technique To Bivariate Marshall-Olkin Family, Hanan. M. Aly, Ola. A. Abuelamayem

Journal of Statistics Applications & Probability

Different bivariate lifetime distributions are used to analyze lifetime data and study the reliability of the products. Sometimes, we find more than one distribution that fit the data well. In this case, we should select the best one. That is why the discriminant analysis is used. In literature, there is only one method used for bivariate lifetime distributions, which is the likelihood ratio test. In this paper, we try to generalize the ratio of minimized Kullback-Leibler divergence to be used as a discrimination method in the bivariate case and it could be applied on the bivariate Marsall-Olkin family. we will …

Insect Cell Expression And Purification Of Recombinant Sars-Cov-2 Spike Proteins That Demonstrate Ace2 Binding, Lucas R. Struble, Audrey L. Smith, William E. Lutz, Gabrielle Grubbs, Satish Sagar, Kenneth W. Bayles, Prakash Radhakrishnan, Surender Khurana, Dalia El-Gamal, Gloria E. O. Borgstahl

Journal Articles: Pathology and Microbiology

The COVID-19 pandemic caused by SARS-CoV-2 infection has led to socio-economic shutdowns and the loss of over 5 million lives worldwide. There is a need for the identification of therapeutic targets to treat COVID-19. SARS-CoV-2 spike is a target of interest for the development of therapeutic targets. We developed a robust SARS-CoV-2 S spike expression and purification protocol from insect cells and studied four recombinant SARS-CoV-2 spike protein constructs based on the original SARS-CoV-2 sequence using a baculovirus expression system: a spike protein receptor-binding domain that includes the SD1 domain (RBD) coupled to a fluorescent tag (S-RBD-eGFP), spike ectodomain coupled …

Catalytic Enantioselective Birch–Heck Sequence For The Synthesis Of Phenanthridinone Derivatives With An All-Carbon Quaternary Stereocenter, Mary Elizabeth Sexton, William Paul Malachowski, Glenn P. A. Yap, Diana Rachii, Greg Feldman, Andrew T. Krasley, Zhilin Chen, My Anh Tran, Kalyn Wiley, Alexandra Matei, Samantha Petersen, Sabrina Tran Tien

Chemistry Faculty Research and Scholarship

Novel phenanthridinone analogues with an all-carbon quaternary stereocenter have been enantioselectively synthesized using the Birch–Heck sequence. Flat phenanthridinone structures have extensive bioactivity but consequently also suffer from poor therapeutic selectivity. The addition of a quaternary center to the phenanthridinone skeleton has the potential to generate more complex analogues with improved selectivity. Unfortunately, no general synthetic pathway to such derivatives exists. Herein we report a four-step process that transforms inexpensive benzoic acid into 22 different quaternary carbon-containing phenanthridinone analogues with a variety of substituents on all three rings: alkyl groups at the quaternary center; methyl, methoxymethyl, or para-methoxybenzyl on the …

At-Home Blood Collection And Stabilization In High Temperature Climates Using, Lauren G Brown, Amanda J Haack, Dakota S Kennedy, Karen N Adams, Jennifer E Stolarczuk, Meg G Takezawa, Erwin Berthier, Sanitta Thongpang, Fang Yun Lim, Damien Chaussabel, Mathieu Garand, Ashleigh B Theberge

Faculty Research 2022

Expanding whole blood sample collection for transcriptome analysis beyond traditional phlebotomy clinics will open new frontiers for remote immune research and telemedicine. Determining the stability of RNA in blood samples exposed to high ambient temperatures (>30°C) is necessary for deploying home-sampling in settings with elevated temperatures (e.g., studying physiological response to natural disasters that occur in warm locations or in the summer). Recently, we have developed

Development Of New Photochemical Tools For Applications In Cancer Research And Enzymatic Signaling, Nicholas Paul Toupin

Wayne State University Dissertations

Photochemical tools empower researchers to act with precision and control over the cellular environment through production of reactive oxygen species (ROS), targeted drug delivery, and modulation of enzymatic activity. These molecular tools use light as a readily available, biorthogonal reagent for initiating advantageous light-activated reactions. Using light in this way allows researchers to affect the cellular environment in specific ways with high spatio-temporal control. This dissertation focuses on expanding the applications of, mostly Ru(II)-based, photoactivated chemicals. Complexes of this type are able to act as photosensitizers in photodynamic therapy (PDT), as photocages (PCT), or as dual-action compounds capable of both …

The Imaginal Lexicon Of Aging Studies, William Ebenstein

Publications and Research

Aging Studies focuses on the cultural, humanistic and experiential aspects of aging insofar as these are already embedded in literature, art, language and everyday life. The lexicon of this multi-disciplinary field has evolved in response to shifts in cultural attitudes, social roles, and conceptual frameworks. At the same time, many age-related words have links to poetic imagery and ancient myths, and to evocative etymologies that conjure an historical context. The article looks at the ongoing and surprisingly difficult effort to find the right word to call an older person. It reviews the language and literary genres associated with longevity. And, …

The Law And Science Of Eyewitness Evidence, Thomas D. Albright, Brandon L. Garrett

Faculty Scholarship

Eyewitness evidence crucially depends on visual perception and memory, which are quite fallible. The potential inaccuracy of eyewitness memory has been long demonstrated in cases of wrongful conviction. Today, decades of scientific research on visual perception and memory have identified key causes of error and methods for improving eyewitness performance. Eyewitness evidence, where identification procedures themselves involve experiments, lends itself to scientific research as do few other areas in law. As a result, eyewitness evidence has become a testing ground for the use of science to inform the law. This Article examines how legal actors—state and federal courts, state lawmakers, …

Structural Studies Of The Nucleolar Stages Of Ribosome Biogenesis In Yeast, Linamarie Miller

Student Theses and Dissertations

The ribosome is the RNA-protein machine responsible for the essential task of translating mRNA into proteins. Ribosomes are heterodimers made up of a small subunit (SSU, 40S) and a large subunit (LSU, 60S). At the interface of these subunits, the mRNA is decoded by the small subunit and peptide bond formation is catalyzed by the rRNA of the large subunit. The cell therefore requires the timely and accurate assembly of functional ribosomal subunits, a complex process termed ribosome biogenesis. In addition to the ribosomal RNAs and ribosomal proteins that make up the mature subunits, eukaryotic cells require over 200 trans-acting …

Clerodendrum Serratum Extract Attenuates Production Of Inflammatory Mediators Inovalbumin-Induced Asthma In Rats, Poonam Arora, Shahid Hamid Ansari, Lalit Mohan Nainwal

Turkish Journal of Chemistry

In the present study, ethanolic extract of Clerodendrum serratum roots was investigated for its potential to reverse some features of bronchial asthma in ovalbumin-induced murine model of asthma. Clerodendrum serratum commonly called bharangi, (family Solanaceae) is a well-known anti-allergic drug in Asian folk system of medicines. In the present work, pharmacological studies are done to provide scientific evidence for therapeutic potential of plant in allergic asthma. Asthma was induced in experimental rats with allergen suspension of ovalbumin and aluminum hydroxide followed by treatment with dexamethasone (2.5 mg/kg, po) or C. serratum root extract (0.53 and 5.3 mg/kg, b. w., po). …

Fluorometric Determination Of Ornidazole By Using Bsa Coated Copper Nanoclusters As A Novel Turn Off Sensor, Mehmetcan Bi̇lkay, Hayri̇ye Eda Şatana Kara

Turkish Journal of Chemistry

A fluorescent probe based on bovine serum albumin stabilized copper nanoclusters (BSA-CuNCs) was developed for the selective and sensitive determination of ornidazole (ORN). The nanoclusters were synthesized via a one-pot hydrothermal process in basic media. The synthesized and characterized BSA-CuNCs have less than 3 nm particle size and exhibited blue emission at 405 nm when excited at 325 nm. Synthesized and characterized nanoclusters were successfully applied as a turn-off fluorescent probe for the determination of ORN in pharmaceutical dosage forms. The quenching mechanism created an was inner filter effect (IFE). The method was linear in the concentration range of 0.52-13.56 …

Synthesis, Antioxidant Activity, Molecular Docking And Adme Studies Of Novel Pyrrolebenzimidazolederivatives, Fi̇kri̇ye Zengi̇n Karadayi, Rahman Başaran, Mehmet Murat Kişla, Bi̇nay Eke, Zeynep Alagöz

Turkish Journal of Chemistry

Several 5-(alkylsulfonyl)-1-substituted-2-(1H-pyrrol-2-yl)-1H-benzo[d]imidazole derivatives were synthesized and their antioxidant activities were investigated using lipid peroxidation (LPO) and 7-ethoxyresorufin O-deethylase (EROD) assays. Docking analysis with Human NAD[P]H-Quinone oxidoreductase 1 (NQO1) was also performed to gather thorough information about these compounds that have antioxidant activities. Moreover, their molecular descriptors and ADME properties were calculated using the SwissADME online program. As a result, most of our compounds possessed better affinity and created ample interactions with NQO1. The most potent compound 5j had LP inhibition value of 3.73 nmol/mg/min. Other compounds exhibited moderate activity on LP levels comparing to standard butylated hydroxy toluene (BHT). However, …

Synthesis, Characterization, And Antimicrobial Evaluation Of Polyvinylalcohol-Osthol Composite Films, Ishrat Gowsia, Feroz Mir, Javid Banday

Turkish Journal of Chemistry

Although poly-vinyl alcohol (PVA) has certain mechanical drawbacks such as a weak barrier, it has widely been used in food packaging over the last many years. To increase the suitability of PVA (C₂H₄O)n and render it ideal for food packaging, a diversity of studies have already been carried out. In the below-mentioned script, we, for the first time, report the use of natural product osthol in making a new composite with PVA for enhancing thermal, physicochemical, and antimicrobial properties. The significant aim of the report is the insertion of osthol (C₁₅H₁₆O_{3 …}

Encapsulation Of Halocadmate Anion Via Hydrogen Bonding: Synthesis And Characterization Of Bis(Diethylenetriamine)Cobalt(Iii) Complex Containing Hexabromocadmate Anion, Vinit Prakash, Ritu Bala, Amrinder Kaur, Daron E. Janzen

Turkish Journal of Chemistry

In this work, the encapsulation of [CdBr₆]^4- by six cations, [Co(dien)₂]³⁺ has been described with the help of single crystal X-ray structural study in the complex, mer-[Co(dien)₂]₂[CdBr₆]Br₂. The complex anion, [CdBr₆]^4- has been obtained through solution method while attempting to synthesize complex dianion, [CdBr₄]^2-. This newly synthesized complex has been initially characterized by elemental analyses and spectroscopic studies (IR, NMR and UV-Visible). IR and NMR studies have been used for the isomeric identification of [Co(dien)₂]^{3+ …}

Transcranial Magnetic Stimulation For Post-Traumatic Stress Disorder, Amber N. Edinoff, Tanner L. Hegefeld, Murray Petersen, James C. Patterson, Christopher Yossi, Jacob Slizewski, Ashley Osumi, Elyse M. Cornett, Adam M. Kaye, Jessica S. Kaye, Vijayakumar Javalkar, Omar Viswanath, Ivan Urits, Alan David Kaye

School of Pharmacy Faculty Articles

Post-traumatic stress disorder (PTSD) is a psychiatric disorder that causes significant functional impairment and is related to altered stress response and reinforced learned fear behavior. PTSD has been found to impact three functional networks in the brain: default mode, executive control, and salience. The executive control network includes the dorsolateral prefrontal cortex (DLPFC) and lateral PPC. The salience network involves the anterior cingulate cortex, anterior insula, and amygdala. This latter network has been found to have increased functional connectivity in PTSD. Transcranial Magnetic Stimulation (TMS) is a technique used in treating PTSD and involves stimulating specific portions of the brain …

A Nationally Representative Survey Assessing Restorative Sleep In Us Adults, Rebecca Robbins, Stuart F. Quan, Daniel Buysse, Matthew D. Weaver, Matthew P. Walker, Christopher L. Drake, Kristen Monten, Laura K. Barger, Shantha M. W. Rajaratnam, Thomas Roth, Charles A. Czeisler

Sleep Medicine Articles

Restorative sleep is a commonly used term but a poorly defined construct. Few studies have assessed restorative sleep in nationally representative samples. We convened a panel of 7 expert physicians and researchers to evaluate and enhance available measures of restorative sleep. We then developed the revised Restorative Sleep Questionnaire (REST-Q), which comprises 9 items assessing feelings resulting from the prior sleep episode, each with 5-point Likert response scales. Finally, we assessed the prevalence of high, somewhat, and low REST-Q scores in a nationally representative sample of US adults (n= 1,055) and examined the relationship of REST-Q scores with other sleep …

Analysis Of Hematocrit Levels In Walleye Sander Vitreus From Similar Waterbodies, Alyssa Hauser

Journal of Earth and Life Science

Walleye Sander vitreus are a popular fish among anglers and hold a prominent place in Minnesotan’s identity as their state fish. Because of this, multiple management practices are used to ensure the continued health of their populations within Minnesota waters. In this study, the process of analyzing hematocrit levels was used to determine if the analysis of hematological parameters could be a useful tool in detecting differences in population health. To evaluate this, blood samples were acquired from age-0 and age-1 fish from two similar lakes and run through a hematocrit centrifuge to separate platelets from plasma to read the …

Codis: Community Detection Via Distributed Seed-Set Expansion On Graph Streams, Austin Anderson

Master's Projects

Community detection has been and remains a very important topic in several fields. From marketing and social networking to biological studies, community detec- tion plays a key role in advancing research in many different fields. Research on this topic originally looked at classifying nodes into discrete communities, but eventually moved forward to placing nodes in multiple communities. Unfortunately, community detection has always been a time-inefficient process, and recent data sets have been simply to large to realistically process using traditional methods. Because of this, recent methods have turned to parallelism, but all these methods, while offering sig- nificant decrease in …

Expression And Purification Of A Cleavable Recombinant Fortilin From Escherichia Coli For Structure Activity Studies, Maranda S. Cantrell, Jackson D. Wall, Xinzhu Pu, Matthew Turner, Luke Woodbury, Owen M. Mcdougal, Lisa R. Warner

Chemistry and Biochemistry Faculty Publications and Presentations

Complications related to atherosclerosis account for approximately 1 in 4 deaths in the United States and treatment has focused on lowering serum LDL-cholesterol levels with statins. However, approximately 50% of those diagnosed with atherosclerosis have blood cholesterol levels within normal parameters. Human fortilin is an anti-apoptotic protein and a factor in macrophage-mediated atherosclerosis and is hypothesized to protect inflammatory macrophages from apoptosis, leading to subsequent cardiac pathogenesis. Fortilin is unique because it provides a novel drug target for atherosclerosis that goes beyond lowering cholesterol and utilization of a solution nuclear magnetic resonance (NMR) spectroscopy, structure-based drug discovery approach requires milligram …

A Novel Phase And Spectroscopic Imaging Technique To Evaluate Cellular Functions, Ting Chean Khoo

Legacy Theses & Dissertations (2009 - 2024)

This thesis presents the application of Raman spectroscopy, digital holographic microscope (DHM) and transport intensity equation (TIE) in imaging biological samples. Raman spectroscopy is a non-destructive technique that can provide chemical structure, concentration, temperature, and molecular interactions. On the other hand, DHM and TIE provide physical measurements such as height, width, area and volume.We applied Raman spectroscopy to study iron-bound transferrin (Tf) in intact human breast cancer cells. Iron is an essential element required for human life and is highly regulated in the body. As the exact mechanisms of iron-bound Tf in cells are not well known, we developed a …

The Photo-Transformation Of Free Methionine In The Presence Of Surrogate And Standard Isolate Dissolved Organic Matter Under Sunlit Irradiation, Benjamin J. Mohrhardt

Dissertations, Master's Theses and Master's Reports

Sulfur (S)-containing amino acids are key sources of carbon, nitrogen, and sulfur involved in protein synthesis, protein function, and providing energy for microbial growth. Dissolved free and combined methionine is one of two S-containing amino acids incorporated into proteins and has been attributed to their stability and function. The oxidation of methionine has received considerable attention given its ubiquitous presence in most biological systems and has been associated with losses in protein function and pathological disorders. In natural waters, methionine is rapidly and selectively taken up by microorganisms to achieve cellular requirements of carbon, nitrogen, and sulfur. The abiotic transformation …

Completing Single-Cell Dna Methylome Profiles Via Transfer Learning Together With Kl-Divergence, Sanjeeva Dodlapati, Zongliang Jiang, Jiangwen Sun

Computer Science Faculty Publications

The high level of sparsity in methylome profiles obtained using whole-genome bisulfite sequencing in the case of low biological material amount limits its value in the study of systems in which large samples are difficult to assemble, such as mammalian preimplantation embryonic development. The recently developed computational methods for addressing the sparsity by imputing missing have their limits when the required minimum data coverage or profiles of the same tissue in other modalities are not available. In this study, we explored the use of transfer learning together with Kullback-Leibler (KL) divergence to train predictive models for completing methylome profiles with …