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Raising Red Flags: Exploring Phenotypic Change In Tiger Moths (Apantesis) Of Coastal Sc Over 50 Years' Time, Allison K. Fletcher

Senior Theses

Organismal phenotypes play a critical role in ecological interactions and individual fitness, and they may undergo rapid shifts through evolutionary adaptation or phenotypic plasticity in response to climate change and environmental disturbances. As morphological traits such as body size, melanization, and aposematic coloration are especially significant for thermoregulation and predator deterrence, it is important to understand the ways in which these traits may be changing on a regional level. Here, I examined the change in phenotype in populations of three tiger moth (Apantesis) species from a single site in coastal South Carolina over a fifty-year period, testing for differences in …

Host P53 Expression During C. Parvum Infection…Does The Virus Play A Role?, Jasdeep Kaur Dhanoa

Biochemistry and Molecular Biology

C. parvum infection induces significant host cellular stress responses, particularly through the modulation of p53 expression underscoring its potential involvement in gastrointestinal malignancies. Understanding these mechanisms is crucial for developing targeted therapeutic interventions. This study investigated the effects of C. parvum infection on host p53 dynamics in HCT-8 cells and assessed the impact of antiviral agents Lamivudine and Amantadine on the virus CSpV1 present within the parasite C. parvum. Further, the study focuses on whether the p53 effect changes with the presence or absence of CSpV1. The p53 assay results showed a constant increase in p53 activity after infection of …

Basking In Abundance: Distribution And Abundance Of Alabama Map Turtles (Graptemys Pulchra) In The Upper Coosa River, Abigail Wilkins

Theses

The Alabama Map Turtle (Graptemys pulchra) is one of the least studied species of turtle in the United States. Little is known about the species distribution and abundance within the northeastern portion of its range. The aim of this study is to determine the distribution of G. pulchra in the Upper Coosa River drainage as well as estimate population sizes for G. pulchra at 6 sites accessible in the Upper Coosa River Drainage. I used a variety of methods (e.g., rapid assessment surveys, trapping, mark-resight population estimates) to determine the presence and abundance of the species in the Upper …

Combination Of Hdac And Fyn Inhibitors In Synovial Sarcoma, Kyra Parker

Theses

The SS18-SSX fusion protein is an oncogenic driver in synovial sarcoma. At the molecular level, SS18-SSX functions as both an activator and a repressor to coordinate transcription of different genes responsible for tumorigenesis. Here, we identify the proto-oncogene FYN as a new SS18-SSX target gene and examine its relation to synovial sarcoma therapy. FYN is a tyrosine kinase that promotes cancer growth, metastasis and therapeutic resistance, but SS18-SSX appears to negatively regulate FYN expression in synovial sarcoma cells. Using both genetic and histone deacetylase inhibitor (HDACi)-based pharmacologic approaches, we show that suppression of SS18-SSX leads to FYN reactivation. In support …

Commonly Encountered Insect Pests Of Virginia, Angel Almaas

Department of Entomology: Distance Master of Science Projects

Foreword

The goal of this pamphlet is to provide an outline of over 50 commonly encountered pest species that occur in Virginia. Care was given to represent each category of pest. This is by no means an exhaustive list of insect pests in Virginia. However, the profiles chosen are reflective of data from insect inventories performed on military bases in coastal Virginia (Fort Eustis, Langley Air Force Base, and Camp Pendleton), sampling from counties in central Virginia, insect species diversity surveys performed in northern Virginia, annual species counts of vector surveillance data from the Virginia Department of Health, and information …

A Comprehensive Catalog Of The Important Insects Found Within The Usa's National Parks, Raven Patrick

Department of Entomology: Distance Master of Science Projects

To fulfill the Masters in Entomology Masters Project requirement, I chose to focus on creating a list of the important insects found within the National Parks of the United States. Important insects, although subjective, was grouped into four categories for my project: Keystone Insect Species, Invasive Insect Species, Insect Species of Conservation Concern, and Culturally Significant Insect Species. Keystone insects include those that are important parts of ecosystems and who which without would cause a significant upset to the food web. Invasive insect species includes those that are introduced into parks accidentally, usually through anthropogenic means, and are causing ecological …

Marine Animalia Diversity And Coral Reef Condition At Big Creek Beach And Boca Del Drago, Bocas Del Toro, Panamá: A Decade Of Change, Lucy Cornett

Panama: Tropical Ecology, Marine Ecosystems, and Biodiversity Conservation

As the oceans’ largest biological structure, coral reefs provide vital ecosystem services, including coastline production, food production, tourism, and preservation of biodiversity. The calm waters provided by the Bocas del Toro archipelago protection from the Caribbeans Sea promotes many coral reefs because of the calmer waters caused by protection from the Caribbean Sea. While many reefs are present, increases in tourism and other anthropogenic threats make monitoring the health of these coral reefs and the diversity of marine organisms of the utmost importance. This study examines the percent substrate cover and the diversity and density marine organisms of two coral …

Sheep Production, Profitability And Greenhouse Gas Emission Of Mixed Farming Systems In Western Australia's Wheatbelt, Tafesse Estifanos, Amir Abadi Abadi Ghadim, Kate Pritchett, Christophe D'Abbadie, Johnny Machon, Paul Mattingley, Bradley Plunkett, Gus Manatsa, Kerrine Blenkinsop, Ross Kingwell, Clinton K. Revell Dr

Animal production and livestock research reports

The south-western agricultural area of Western Australia is a vital contributor to Australia's agricultural output. It generates over $10 billion annually in agricultural production from 2017–18 to 2021–22 and more than $8 billion in export value over the same period. Mixed farming systems characterise the region, producing broadacre crops (primarily wheat, barley, and canola) and livestock. The region's livestock industry is dominated by sheep production for meat and wool, and the state’s share of the national sheep flock in 2020–21 was at 18%.

This report provides a comprehensive review and industry analysis of sheep production in WA, its economic significance, …

Life Line Spring 2025, Otterbein Biology And Earth Science Department

Life Line - The Biology Department Newsletter

In this issue:

Greetings and Farewell!; COSI 2024; Ketchup, Culture, and an Unforgettable Conference; Alumna Earns PhD; Tortoises, Butterflies, and Cockroaches, Oh My!; Licking River Roundup 2024; Aquarium Students Explore Coral Restoration in Fiji; The Galapagos Islands: Geology, Ecology, Conservation, and Culture; Dr. Svitana Presents on Otterbein Lake Well Field; Cell Bio Pilots Unique Approach to Learning; Hoggarth to Retire, June 2025; AED is BACK on Campus!; The Greenhouse Gets a Lifeline; Acoustic Rabies Identification (ARID): A Sound-Based Approach to Rabies Testing

Using Camera Stations To Investigate Occupancy Probability And Habitat Associations Of Island Spotted Skunks (Spilogale Gracilis Amphiala) On Santa Rosa And Santa Cruz Islands, Desirae Thomaier

Master's Theses

The California Channel Islands are unique in that two islands (Santa Rosa and Santa Cruz) support two endemic mesocarnivores, the island fox (Urocyon littoralis) and the island spotted skunk (Spilogale gracilis amphiala). Following the near-extinction of island foxes in the early 2000s, island spotted skunk populations increased significantly. Now that foxes have recovered, skunk captures have precipitously declined - possibly due to interspecific competition with foxes who have a similar diet but a larger body size and wider temporal niche. It has been proposed that skunks selectively use canyon bottoms and drainages with high vegetation cover and that these areas …

Creatine Supplementation Is Safe, Beneficial Throughout The Lifespan, And Should Not Be Restricted, Richard Kreider, Andrew Jagim, Jose Antonio, Douglas Kalman, Chad Kerksick, Jeffrey R. Stout, Robert Wildman, Rick Collins, Diego A. Bonilla

Faculty Scholarship

As researchers investigating creatine supplementation, we have become increasingly concerned about reports that government agencies are attempting to restrict the sale of dietary supplements, including dietary supplements containing creatine, to children and adolescents. Creatine is a naturally occurring compound found in every cell in the human body that plays a critical role in cellular metabolism. The daily turnover of creatine is about 2–4 grams/day, depending on muscle mass and physical activity levels (1, 2). About half of the daily need for creatine is synthesized in the body from amino acids (arginine, glycine, methionine) and stored as free creatine or phosphocreatine …

Comparative Performance Of Reciprocal Cross Hybrid Striped Bass In Three Intensive Production Systems, Bartholomew Green, Steven D. Rawles, Matthew E. Mcentire, Candis L. Ray

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Objective: The objective of these two studies was to quantify the comparative performances of yearling and 2- year- old reciprocal cross hybrid Striped Bass (White Bass Morone chrysops ♀ × Striped Bass M. saxatilis ♂), also called sunshine bass, and water quality in traditional and split ponds and an outdoor mixotrophic biofloc technology production system.
Methods: In study 1, two- year- old fish (247.9 g/fish) were stocked at 7,410 fish/ha (0.86 fish/m3) in ponds and 6.3 fish/m2 (7.1 fish/m3) in biofloc system tanks and grown for 151–153 d. In study 2, yearling fish (147.0 g/fish) were stocked at 7,904 fish/ha …

Food For Thought: The Impact Of The Gut-Brain Axis On Mental Health, Emily S. Miiller

Honors Thesis

Research regarding the role of the human microbiome has grown over the years as professionals develop a better understanding of the gut-brain axis. New studies and expanding knowledge on the relationship between the nervous system and gut microbiome promote a deeper understanding of the impact of diet on mental health. Diet plays a crucial role in maintaining a healthy balance between the gut and the brain. Incorporating nutritional modulators, such as probiotics, prebiotics, and vitamins, is crucial for supporting the gut-brain axis by influencing neurotransmitter production, managing inflammation, and promoting the growth of beneficial gut bacteria. On the other hand, …

Investigating Mercury Contamination In Urban Fish: Atomic Absorption Spectroscopy And Histological Analysis For Environmental And Human Health, Indy L. Cockrill, Ross Andrew

SPARK Symposium Presentations

Mercury contamination in aquatic ecosystems is a pressing environmental and public health concern, particularly in urban areas where industrial runoff and wastewater contribute to heavy metal accumulation. This study investigates mercury presence in fish from Richland Creek in Nashville, TN, by analyzing histological changes in liver and muscle tissues. Mercury bioaccumulates in aquatic food chains, potentially leading to physiological damage in fish and posing health risks to humans who consume contaminated seafood. While prior research has established mercury’s toxic effects, there is a need for localized studies assessing its presence in urban waterways. The hope is to find histological abnormalities …

Herbicide Management For Bromus Tectorum To Enhance Rangeland Productivity In Western Nebraska, Miranda Mueller

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Cheatgrass, Bromus tectorum (L.), has increased in the mixed-grass prairies of the Nebraska Panhandle. Limited research is available in these ecosystems on the ecological factors that influences its spread and options for management. This study focuses on the efficacy of herbicide treatments (indaziflam and imazapic) as a cheatgrass control method on the native rangelands of western Nebraska. In September of 2022, a mixture of indaziflam (73 g ai ha^-1, Rejuvra) and imazapic (70 g ai ha^-1, Plateau) was aerially applied at 6 unique upland ecological sites (1-3 ha) near Scottsbluff, Nebraska. Functional group plant biomass data …

Machine Learning Predicts Non-Preferred And Preferred Vertebrate Hosts Of Tsetse Flies (Glossina Spp.) Based On Skin Volatile Emission Profiles, Olabimpe Y. Orubuloye, David P. Tchouassi, Abdullahi A. Yusuf, Christian W.W. Pirk, Daniel K. Masiga, Edward Kariuki, Baldwyn Torto

All Peer-Reviewed Publications

Tsetse fly vectors of African trypanosomosis preferentially feed on certain vertebrates largely determined by olfactory cues they emit. Previously, we established that three skin-derived ketones including 6-methyl-5-hepten-2-one, acetophenone and geranyl acetone accounted for avoidance of zebra by tsetse flies. Here, we tested the hypothesis that these three ketones serve as biomarkers for tsetse flies to distinguish between non-preferred- and preferred-vertebrate hosts. We used coupled gas chromatography/mass spectrometry to analyze and compare the skin volatile emissions of two non-preferred- (waterbuck and zebra) and four preferred- (buffalo, donkey, horse, warthog) vertebrate hosts in two wildlife parks in Kenya. We detected a total …

Defatted Black Soldier Fly Larvae Meal As A Substitute Of Soybean Meal In Dairy Cow Diets, D. J.M. Braamhaar, W. F. Pellikaan, D. List, D. Korir, C. M. Tanga, S. J. Oosting

All Peer-Reviewed Publications

The replacement of soybean meal (SBM) with insect meal, e.g. black soldier fly larvae meal (BSFLM), as an alternative protein source in livestock feed is gaining traction globally. To date, no in vivo studies have been conducted to test the impact of BSFLM on the milk production of dairy cows. This study quantified the effects of replacing SBM with defatted black soldier fly larvae meal (DBSFLM) in diets of lactating dairy cows on feed intake, milk production and composition, apparent total-tract digestibility, feed- and nitrogen use efficiency. Twelve lactating Holstein-Friesian cows were used in a replicated 3 × 3 Latin …

Soluble Cd13 Is A Potential Mediator Of Neutrophil-Induced Thrombogenic Inflammation In Sars-Cov-2 Infection, Pei-Suen Tsou, Ramadan A. Ali, Chenyang Lu, Gautam Sule, Carmelo Carmona-Rivera, Serena Lucotti, Yuzo Ikari, Qi Wu, Phillip L. Campbell, Mikel Gurrea-Rubio, Kohei Maeda, Sharon E. Fox, William D. Brodie, Megan N. Mattichak, Caroline Foster, Ajay Tambralli, Srilakshmi Yalavarthi, M Asif Amin, Katarina Kmetova, Bruna Mazetto Fonseca, Emily Chong, Yu Zuo, Michael D. Maile, Luisa Imberti, Arnaldo Caruso, Francesca Caccuri, Virginia Quaresima, Alessandra Sottini, Douglas B. Kuhns, Et Al

School of Medicine Faculty Publications

The soluble variant of the ectopeptidase CD13 (sCD13), released from the cell surface by matrix metalloproteinase 14 (MMP14), is a potent pro-inflammatory mediator, displaying chemotactic, angiogenic, and arthritogenic properties through bradykinin receptor B1 (B1R). We revealed a link between sCD13 and amplified neutrophil-mediated inflammatory responses in SARS-CoV-2 infection. sCD13 was markedly elevated in patients with COVID-19 and correlated with disease severity and variants, ethnicity, inflammation markers, and neutrophil extracellular trap formation (NETosis). Neutrophils treated with sCD13 showed heightened NETosis and chemotaxis, which were inhibited by sCD13 receptor blockade. Meanwhile sCD13 did not induce platelet aggregation. Single-cell analysis of COVID-19 lungs …

Climate And Crop Modeling For New England Agricultural Climate Change Adaptation, Madeline K. Wolfe

Geography Undergraduate Senior Theses

As climate change brings increased temperatures and precipitation variability to New England, it is imperative to understand the ways that farmers make decisions about agricultural climate change adaptation for their corn farms. Dairy farming is the largest agricultural industry in New England, with corn being a primary crop to feed these cattle. This project seeks to understand how climate modeling and crop modeling are and are not useful for these corn farm decisions, and contributes research about what types of information are useful and available to farmers making adaptation decisions. Crop modeling over New England reveals that corn farming in …

Impact Of Diet Quality And Functional Food (Honey) On Gut Microbiota And Metabolic Health In Obesity, Sarah M. Eaton

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Understanding the impact of dietary patterns and functional foods on human health is crucial for the advancement of evidence-based nutrition choices. This dissertation investigates the effects of three a priori-defined healthful dietary patterns as well as a functional food, specifically Manuka honey, on gut health and metabolic markers in individuals with overweight and obesity. Two studies were conducted to explore these relationships. The first study, a post-hoc, cross-sectional analysis, compared the effects of three healthful dietary patterns: Healthy Eating Index-2010 (HEI-2010), Dietary Approaches to Stop Hypertension (DASH), and Mediterranean Dietary Pattern (MDP) on markers of intestinal permeability and gut microbiota …