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Impact Of Diet Quality And Functional Food (Honey) On Gut Microbiota And Metabolic Health In Obesity, Sarah M. Eaton

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Understanding the impact of dietary patterns and functional foods on human health is crucial for the advancement of evidence-based nutrition choices. This dissertation investigates the effects of three a priori-defined healthful dietary patterns as well as a functional food, specifically Manuka honey, on gut health and metabolic markers in individuals with overweight and obesity. Two studies were conducted to explore these relationships. The first study, a post-hoc, cross-sectional analysis, compared the effects of three healthful dietary patterns: Healthy Eating Index-2010 (HEI-2010), Dietary Approaches to Stop Hypertension (DASH), and Mediterranean Dietary Pattern (MDP) on markers of intestinal permeability and gut microbiota …

Dental Disparities: An Analysis Of Dental Caries Risk Factors Promoting Disparities Between Rural And Urban Communities In Nebraska, Nick Lounsbery

Honors Program: Senior Projects (Public)

The prevalence of dental caries in the United States remains a public health concern, as it is one of the most common chronic conditions across people of all ages. Research shows that preventative measures including brushing, fluoridated water, and regular checkups are effective strategies to mitigate tooth decay. However, there appears to be a divide between rural and urban populations, as the presence of dental cavities is significantly greater in remote areas in the State of Nebraska. Research methods used in this analysis included analyzing accredited academic literature to illustrate how cavities form from a biochemical perspective. Research methods also …

Comparative Mechanical And Elastic Properties Of The Dorsal And Ventral Tendons In The Peduncle Of Harbor Porpoise (Phocoena Phocoena), Alexa R. Cesari, Jesse K. Placone, Nicole L. Ramo, Michael V. Rosario, Matthew Morris, Danielle S. Adams, Frank E. Fish

Biology Faculty Publications

Cetaceans swim via vertical movements of the tail. The tendons located in the caudal peduncle are attached to the caudal vertebrae to generate propulsive oscillations. Arguments have centered on whether the upstrokes and downstrokes of the tail and propulsive flukes are symmetrical or asymmetrical in time. Previous research from kinematics of swimming animals, muscle architecture and histology has supported both conditions. However, the composition and structure of the tendons suggest a potential mechanism to evaluate this disparity. In this study, the tendons of the caudal peduncle of the harbor porpoise (Phocoena phocoena) – specifically, the extensor caudae medialis …

Automated Plant Morphological Assessment And Microbial Community Monitoring In Simulated Lunar Regolith, Collin R. Topolski

Doctoral Dissertations and Master's Theses

Upcoming lunar bases will need to leverage in-situ resources to ensure long-term sustainability and reduce mission costs. One key challenge is food production, which could be addressed by growing crops in lunar regolith-based substrates. However, lunar regolith lacks essential nutrients, requiring organic amendments to support continuous plant cultivation. This study examined the impact of manure-amended lunar regolith simulant on Mizuna mustard growth while assessing microbial community diversity and functionality. To support this effort, a custom software pipeline utilizing an open-source library, PlantCV, was developed to automatically track plant leaf area and height via image analysis. Results indicated that amended rhizosphere …

Perceptions Of Internship Experiences For Environmental Science Undergraduates, Sydney Brock

Senior Theses

Internships are widely recognized as valuable components of undergraduate education, yet their accessibility, impact, and role in shaping student development vary significantly across disciplines and individual experiences. This thesis investigates the undergraduate internship experience within the School of the Earth, Ocean, and Environment (SEOE) at the University of South Carolina, drawing on survey data and reflective responses from students who participated in internships between 2022 and 2024. The study explores the professional, academic, and personal benefits of internships, with particular attention to how these experiences influence students' career direction, confidence, and skill development. Findings indicate that regardless of format, duration, …

Individual Differences In The Effects Of A Muscarinic Antagonist On Cued Fear Recall And Extinction, Caitlyn J. White

Senior Theses

Post-Traumatic Stress Disorder (PTSD) is characterized by an inability to extinguish fear after experiencing a traumatic event. The cholinergic system shows increased activity during fear learning and extinction, suggesting a role for cholinergic transmission in PTSD. Our lab has previously found that rats can be divided into extinction resistant (ER) and extinction competent (EC) phenotypes, with ER representing a PTSD-like phenotype. While women are more likely to develop PTSD, there is limited literature on the cholinergic system and recall of cued fear or extinction in female rats which are used as a model system due to their similar brain anatomy. …

Eating The Fat Of The Land: The Work Of Coxiella Burnetii To Traverse Intracellular Lipid Landscapes, Charlie Porto

Theses & Dissertations

Coxiella burnetii is an obligate intracellular pathogen and the causative agent of Q fever in humans. With primary hosts in livestock ruminants, C. burnetii is endemic globally, presenting significant health risks to agricultural workers and herd health at large. Numerous wild animal reservoirs exist, increasingly threatened by expanding livestock ranges. Furthermore, with a low infectious dose through aerosol transmission, the CDC considers C. burnetii a potential bioweapon, though in humans a large amount of infections persist subclinically, occasionally progressing to pneumonia and hepatitis with chronic infections leading to endocarditis.

The parasitic life cycle of C. burnetii is biphasic, a small …

College Of Natural Sciences Newsletter, Spring 2025, College Of Natural Sciences

College of Natural Sciences Newsletters and Reports

Page 1 Dean's Message from Dr. Sen Subramanian
Page 2 Student Spotlights
Page 3 Kaushik honored, Three-Minute Thesis, & Ice Cores revealed
Page 4 Alumni Spotlight
Page 5 Day of Scholars
Page 6 Cyanide research, Record Research Expenditures, Adhikari awarded Miller Research Award
Page 8 Hanson Receives NSF Grant
Page 9 Snow Lab Research Team
Page 10 Faculty Awards
Page 11 Drone Day
Page 13 Khalaf Awarded Presidential Award

Tracking Phenological Shifts Of Black Guillemots Cepphus Grylle In The Gulf Of Maine, Jocelyn L. Little

Student Publications

Rapid warming in the Gulf of Maine has coincided with changes in phenology across trophic levels. Phenological change has yet to be studied in black guillemots (Cepphus grylle), a widely distributed benthic forager that is showing population decline in the region. Because black guillemots nest in burrows, their phenology can be challenging to study. I developed methods to track black guillemot phenology on Petit Manan Island, a seabird breeding colony in the Gulf of Maine. Specifically, I asked Q1) Can daily tower count data be used to predict black guillemot breeding phenology? Q2) How is black guillemot breeding phenology shifting …

Common Terns (Sterna Hirundo) As Bioindicators For Atlantic Herring (Clupea Harengus) Distribution In The Gulf Of Maine, Logan R. Becker

Student Publications

Effective fisheries management is increasingly challenged by climate-driven shifts in species distribution and by a lack of data on early life stages of key forage fish species. In the Gulf of Maine, Atlantic herring (Clupea harengus), a once economically dominant fishery species and a key prey species for many dependent predators, has declined due to poor early-stage recruitment and warming waters. Despite this decline, current trawl surveys do not inform early-stage Atlantic herring distribution due to mesh size limitations, leaving critical gaps in our knowledge. To address this knowledge gap, I used common terns (Sterna hirundo) …

Playful Paws: Are There Sex Differences In Playfulness With Freshwater Otters?, Julianne Pena

Posters - 2025

Personality is crucial for understanding the lives of species because it affects how they survive, the way they move, reactions to diseases, and much more (Merrick & Koprowski, 2017; Wolf & Weissing, 2012). The importance of this research is to understand how biological sex may interact with otter personalities through social interactions amongst other otters, the physical world, and humans. Female and male otters vary in the way that they play, with males playing more rough than females (Green, 2000). Play, which is associated with rock juggling, decreases as otters mature (Alliston et al., 2020). Females used object play more …

Is Less More, Or More Better? Behavioral Insights From Bottlenose Dolphins During Dolphin Interaction Programs, Megan E. Davis, Alicia Virthe, Heather Manitzas Hill, Kathleen Dudzinski, Michelle Samm, Buck Lyman, Kelly Flaherty Clark, Todd Robeck

Posters - 2025

This poster was created for the 5th annual Research Showcase. From the poster:

Using video recordings collected during randomly selected human-dolphin interaction programs (2022-2023), 19 target dolphins were assessed for their behavioral repertoire, correct and incorrect responses to trainer/guest-given cues, and the total number of unique requested behaviors.

Obstacles And Opportunities In Marine Mammals Care And Welfare: Insights From Trainers, Alicia Virthe, Heather Manitzas Hill, Sabrina Brando

Posters - 2025

This poster was created for the 5th annual Research Showcase. From the poster:

The aims of the study included:

Identifying obstacles faced by caregivers

Exploring beliefs hindering the promotion of animal care and welfare

Highlighting the opportunities that caregivers perceive to maintain and enhance animal welfare and care

Do Otters Remember What They Solved? Manyotters - Collaborative Science, Manyotters, Heather Manitzas Hill, Deirdre Yeater, Caroline M. Delong, Catina Wright, Emily Langdon, Lisa Halstead

Posters - 2025

This poster was created for the 5th annual Research Showcase. From the Poster:

The ManyOtters group seeks to promote studies with larger sample sizes and a variety of species of otters, while developing a collaborative network of scientists. ...

Our initial project focuses on long term memory in otters.

For more information about ManyOtters please visit

https://manyotters.github.io/

Many Otters Project - Coding Analysis Of Otter Behavior With Foraged Puzzles, Isaiah Charles, Andres Vega

Posters - 2025

The study of long-term memory and the cognitive abilities of otters is not extensively documented. This research consisted of the use of foraging puzzles, which are different containers with food inside such as fish, squid, and oysters. This research project, named ManyOtters, takes inspiration from the foraging puzzle techniques exhibited in separate studies (e.g., Saliveros et al., 2022; Ladds et al. 2017). Based on the studies, foraging puzzles have shown to draw engagement for several otter species. The species focused on in this study were Asian small-clawed otters (Aonyx cinereus) and African spotted-necked otters (Hydrictis maculicollis) housed at SeaWorld San …

Think Like An Otter: Behavioral Problem-Solving In Fresh-Water Otters, Madison N. Vale, Miranda C. Ibarra

Posters - 2025

This study examines problem-solving in Asian Small-Clawed Otters (ASCOs), focusing on their cognitive processes during puzzle-solving tasks. The sample included two social groups of four otters: Eleanor and Dustin in one group, and Saki and Wonton in the other. Both groups were presented with a series of puzzles, and their responses were video recorded for coding. The relationship between sex and social learning was explored, but no significant findings were observed. Additionally, it was tested whether the number of strategies used and engagement duration predicted success; neither factor was a significant predictor. Overall, the results were inconclusive, offering no clear …

Novel Approaches To Analyze Consumer Behavior And Policies To Promote Sustainable Consumption, Bhagyashree Katare, Xuejian Wang, Shuoli Zhao, Jacqueline Yenerall

Agricultural Economics Faculty Publications

Technological advancements, such as online grocery shopping, have significantly transformed consumer retail environments and experiences. Effectively studying consumer behavior in these new environments requires the use of novel methodological approaches, which will also aid in developing interventions to encourage sustainable consumption. This paper begins by providing an overview of the current literature on novel approaches to analyzing consumer behavior, then provides an empirical application by examining consumer decision-making pathways within an online grocery shopping platform. Specifically, the paper focuses on exploring the consumers’ digital footprints, such as page visits, product additions and removals, and interactions with information labels to identify …

Ecological Studies Of Wolves On Isle Royale, Sarah R. Hoy, John A. Vucetich, Rolf O. Peterson

Ecological Studies of Wolves on Isle Royale

Annual Report 2024-2025

Spring 2025, Humayun Islam

PMI Newsletter

No abstract provided.

What Do We Know About Insect Responses To Global Change? A Review Of Meta-Analyses On Global Change Drivers, Mayra C. Vidal, Mariana Abarca, Kristi Backe, Anne E. Curé, Deborah L. Finke, Amanda M. Koltz, Alycia C.R. Lackey, J. Christina Mitchell, Rebecca M. Prather, Ellen A.R. Welti, Gina M. Wimp, Shannon M. Murphy

Biological Sciences: Faculty Publications

1. Global change is causing major declines in biodiversity, especially of insects. Scientific interest in global change impacts on insects has increased in recent years, resulting in many different meta-analyses examining questions within this topic.

2. We performed a comprehensive review of meta-analyses examining the effects of global change stressors on insects to identify well-studied questions and gaps in our knowledge and synthesise the responses of insects to those stressors. We identified 75 meta-analyses that fit our scope, accounting for 905 meta-results and spanning 18 global change stressors.

3. Our synthesis identified several global change stressors that are relatively well-studied …

Next-Generation Biomedical Implants And Stimuli Responsive Soft Optical Arrays Via Surface Functionalized Elastomeric Materials, John M. Kapitan

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

The demands of emerging technologies, such as soft robotics, stretchable electronics, and next generation biomedical implants, has led to the development of diverse polymeric and hybrid organic/inorganic materials specifically tuned to meet these challenges. These materials typically have low Young’s moduli and are inert with high chemical stability. However, this has led to materials that are static in nature, lacking any meaningful way of responding to changes in their environment and adapting to these changes. This has driven a shift towards the design, manufacturing, and characterization of various stimuli responsive surfaces, relying on precise control of surface chemical coatings and …

Coyote (Canis Latrans) Macronutrient Consumption And Diet Relative To Seasonality And Urbanization, Katherine C. B. Weiss, Sean C. P. Coogan, Pierre Deviche, Jesse S. Lewis, Savage C. Hess, Jan Schipper, Eric G. Strauss, Beckett Sterner

Biology Faculty Works

Diet selection informs the health, fitness, and behavior of wild predators. Due to assumptions that vertebrate prey contains similar compositions of macronutrients (i.e., protein, carbohydrates, and lipids), whole prey items traditionally define carnivore diets. However, increasing evidence suggests that prey differ in terms of their macronutrient compositions, particularly relative to body size. Furthermore, omnivorous predators, like coyotes (Canis latrans), integrate both prey and nonprey diet items whose macronutrient compositions vary. This is particularly important in urbanized systems, which introduce or alter the distributions of prey (e.g., domestic pets) and nonprey (e.g., ornamental plants) foods in ways that contribute …

Steroid Receptors And Coregulators: Dissemination Of Sex Differences And Emerging Technologies, Sally Pauss, Evelyn A. Bates, Genesee J. Martinez, Zane T. Bates, Zachary A. Kipp, Cassandra D. Gipson, Terry D. Hinds Jr.

Markey Cancer Center Faculty Publications

Steroid receptors are ligand-induced transcription factors that have broad functions among all living animal species, ranging from control of sex differences, body weight, stress responses, and many others. Their binding to coregulator proteins is regulated by corepressors and coactivators that interchange upon stimulation with a ligand. Coregulator proteins are an imperative and understudied aspect of steroid receptor signaling. Here, we discuss steroid receptor basics from protein domain structures that allow them to interact with coregulators and other proteins, their essential functions as transcription factors, and other elemental protein–protein interactions. We deliberate about the mechanisms that coregulators control in steroid receptor …

Differential Impacts On Proteoglycan Expression In The Response To Lens Wounding In Reparative And Pro-Fibrotic Microenvironments, Janice L Walker, A. Sue Menko

Department of Pathology, Anatomy, and Cell Biology Faculty Papers

Ex vivo lens epithelial explant cultures created through a technique that mimics cataract surgery provided an ideal model with which to compare the impacts on proteoglycan expression in the response to wounding in both reparative promoting and pro-fibrotic microenvironments. On their native basement membrane capsule the injured lens epithelium undergoes regenerative repair, with the wound closing within a few days. Their migration across the wound area is led by a population of activated lens resident immune cells. The same leader cell population also directs the wounded epithelium to migrate off the outside edges of the lens explant across the surrounding …

Therapeutic Role Of Bioactive Peptides Derived From Hemoglobin, Priya Antony Pereppadan

Dissertations

Bioactive peptides are peptide fragments derived from the proteolytic cleavage or maturation of functional proteins that have shown promising therapeutic potential in addressing major health challenges, including type 2 diabetes, hypertension, and pain management. Diabetes is characterized by high blood glucose levels due to insufficient insulin production or utilization. There are several therapeutic targets for managing diabetes, and one key target is dipeptidyl peptidase IV (DPP IV) due to its role in glucose metabolism. In hypertension, angiotensin-converting enzyme 1 (ACE1) is critical for regulating blood pressure by forming angiotensin II, and its inhibition represents an established treatment strategy. Additionally, the …

Alfred Russel Wallace Notes 36. Wallace, Evolution And Providence., Charles H. Smith

Faculty/Staff Personal Papers

One of Alfred Russel Wallace’s most important contributions to thought was his identification of the key role provident behavior assumes in human evolution. In this essay I set out the way this idea influenced his thought process after 1864, and especially how it affected his appreciation of the possible role of spiritualism in evolution.

Immunotherapy Targeting A Leader Sequence Cathepsin G-Derived Peptide, Chunhua Shi, Ze Tian, Jun Yan, Mao Zhang, Pariya Sukhumalchandra, Edward Chang, Guojun Yang, Junping You, Meng Cui, Qing Shi, Celine Kerros, Anne Philips, Na Qiao, Hiroki Torikai, Sathvik Patchametla, Anna Sergeeva, Lisa St John, Helen He, Dmitri Wiederschain, Benjamin H Lee, Geraldine L C Paulus, Dongxing Zha, Jeffrey Molldrem, Gheath Alatrash

Faculty, Staff and Student Publications

Myeloid azurophil granules provide a rich source of intracellular leukemia antigens. Cathepsin G (CG) is a serine protease that has higher expression in acute myeloid leukemia (AML) blasts in comparison to normal myeloid progenitors. Based on the unique biology of HLA-A*0201 (HLA-A2), in which presentation of leader sequence (LS)-derived peptides is favored, we focused on the LS-CG-derived peptide CG1 (FLLPTGAEA). We previously detected CG1/HLA-A2 complexes on the surface of primary HLA-A2+ AML blasts and cell lines, and immunity targeting CG1/HLA-A2 in leukemia patients. T cell receptor (TCR)-mimic (m) antibodies are immunotherapeutic antibodies that target peptide-HLA (pHLA) complexes. Here we report …

Growing South Dakota (Spring 2025), College Of Agriculture, Food And Environmental Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

[Page] 2 Keeping South Dakota's food supply safe
[Page] 6 Controlling environmental listeria
[Page] 8 Inaugural 2+2 vet program class graduates
[Page] 10 Faculty honored for excellence
[Page] 12 New leader for agronomy, horticulture, plant science
[Page] 14 Students spend spring break in South Africa
[Page] 16 Hadacek offers advice to fellow graduates
[Page] 17 Park Interpretation and Management Club starts
[Page] 18 On the front lines with invasive species
[Page] 22 Director Karla Trautman retires
[Page] 24 Helping navigate precision ag
[Page] 26 Growing bigger, better beef
[Page] 28 Jackrabbits Then and Now

Ccr7 Regulated Mechanisms That Limit The Adaptive Immune Response, Jaisel Amilcar Cervantes

Open Access Theses & Dissertations

C-C chemokine receptor 7 (CCR7) is critical in guiding T cell migration within the thymus and peripheral lymphoid tissues, shaping the adaptive immune response by promoting central tolerance and regulating immune homeostasis. Although its role in lymphocyte trafficking is well established, the molecular mechanisms by which CCR7 influences thymocyte development and T cell receptor (TCR) repertoire formation remain less understood. This study examines how CCR7 deficiency impacts thymic selection, TCR diversity, and the signaling events that shape repertoire restriction. Using a homozygously deleted CCR7 murine model, we found that the absence of CCR7 results in a less restricted TCR repertoire …

The Pharmaceutical Applications Of Poisonous Compounds Found In Datura Stramonium, Ruby M. Raffaldt

Senior Theses

Datura stramonium is a plant that is known for its toxicity. However, a facet of this plant’s noxious chemicals, namely its tropane alkaloids, can be used medicinally. The aim of this paper was to compile knowledge on the botanical, chemical, and medicinal properties of Datura stramonium L with a focus on atropine, scopolamine, and anisodamine; three tropane alkaloid compounds with the ability to be both a poison and a medicine. Information was compiled using pre-existing studies, articles, and literature reviews on D. stramonium. For each of the three alkaloids, their biosynthetic pathways, targets, administration, distribution, metabolism, and excretion were discussed. …