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Heterozygous Kmt2d Loss Diminishes Enhancers To Render Medulloblastoma Cells Vulnerable To Combinatory Inhibition Of Lsd1 And Oxphos, Shilpa S Dhar, Calena Brown, Ali Rizvi, Lauren Reed, Sivareddy Kotla, Constantin Zod, Janak Abraham, Jun-Ichi Abe, Veena Rajaram, Kaifu Chen, Min Gyu Lee May 2025

Heterozygous Kmt2d Loss Diminishes Enhancers To Render Medulloblastoma Cells Vulnerable To Combinatory Inhibition Of Lsd1 And Oxphos, Shilpa S Dhar, Calena Brown, Ali Rizvi, Lauren Reed, Sivareddy Kotla, Constantin Zod, Janak Abraham, Jun-Ichi Abe, Veena Rajaram, Kaifu Chen, Min Gyu Lee

Faculty, Staff and Student Publications

The histone H3 lysine 4 (H3K4) methyltransferase KMT2D (also called MLL4) is one of the most frequently mutated epigenetic modifiers in many cancers, including medulloblastoma (MB). Notably, heterozygous KMT2D loss frequently occurs in MB and other cancers. However, its oncogenic role remains largely uncharacterized. Here, we show that heterozygous Kmt2d loss in murine cerebellar regions promotes MB genesis driven by heterozygous loss of the MB-suppressor gene Ptch via the upregulation of tumor-promoting programs (e.g., oxidative phosphorylation [OXPHOS]). Downregulation of the transcription-repressive tumor suppressor NCOR2 by heterozygous Kmt2d loss, along with Ptch


Overcoming Nk-Mediated Rejection By Anti-3rd-Party Central Memory Veto Cd8 T Cells Through Downregulation Of Dnam-1 On Alloreactive Nk Cells, Wei-Hsin Liu, Aloukick Kumar Singh, Christa Blagdon, Sandeep Kumar Yadav, Einav Shoshan, Esther Bachar-Lustig, Yair Reisner May 2025

Overcoming Nk-Mediated Rejection By Anti-3rd-Party Central Memory Veto Cd8 T Cells Through Downregulation Of Dnam-1 On Alloreactive Nk Cells, Wei-Hsin Liu, Aloukick Kumar Singh, Christa Blagdon, Sandeep Kumar Yadav, Einav Shoshan, Esther Bachar-Lustig, Yair Reisner

Faculty, Staff and Student Publications

Anti-3rd-party central memory veto CD8 T (veto Tcm) cells can overcome T cell-mediated graft rejection under mild conditioning without causing significant graft versus host disease (GVHD). We previously demonstrated that these veto Tcm cells can effectively delete anti-donor T cell clones through a Fas-FasL mechanism, whereas their ability to neutralize alloreactive natural killer (NK) cells and the mechanism of such potential activity remained unknown. Using “nude” mice as recipients of allogeneic T cell-depleted hematopoietic stem cell transplantation (HSCT), we demonstrate effective inhibition of NK-mediated rejection by Tcm cells. Ex vivo studies revealed that Tcm cells express high levels of CD155, …


The Aquatic Symbiosis Genomics Project: Probing The Evolution Of Symbiosis Across The Tree Of Life, Matthew Berriman, Victoria Mckenna, Roxanne Beinart, John M. Archibald, Ute Hentschel, Michael N. Dawson, Jose V. Lopez, Patrick J. Keeling, Jillian M. Petersen, Anne W. Thompson, Multiple Addiional Authors May 2025

The Aquatic Symbiosis Genomics Project: Probing The Evolution Of Symbiosis Across The Tree Of Life, Matthew Berriman, Victoria Mckenna, Roxanne Beinart, John M. Archibald, Ute Hentschel, Michael N. Dawson, Jose V. Lopez, Patrick J. Keeling, Jillian M. Petersen, Anne W. Thompson, Multiple Addiional Authors

Biology Faculty Publications and Presentations

We present the Aquatic Symbiosis Genomics Project, a global collaboration to generate high quality genome sequences for a wide range of eukaryotes and their microbial symbionts. Launched under the Symbiosis in Aquatic Systems Initiative of the Gordon and Betty Moore Foundation, the ASG Project brings together researchers from across the globe who hope to use these reference genomes to augment and extend their analyses of the dynamics, mechanisms and environmental importance of symbioses. Applying large-scale, high-throughput sequencing and assembly technologies, the ASG collaboration will assemble and annotate the genomes of 500 symbiotic organisms – both the “hosts” and the microbial …


Tickborne Colpodella Species Infections: Time For A New Integrated Approach To Understand Transmission And Pathogenicity, T. Y. Sam-Yellowe, Trinity Nims, Sona Qaderi, Mary M. Asraf May 2025

Tickborne Colpodella Species Infections: Time For A New Integrated Approach To Understand Transmission And Pathogenicity, T. Y. Sam-Yellowe, Trinity Nims, Sona Qaderi, Mary M. Asraf

Biological, Geological, and Environmental Faculty Publications

Colpodella species are free-living predatory protists that prey on algae, ciliates and bodonids using myzocytosis. Colpodella species have been reported in human and animal infections. Polymerase chain reaction (PCR) using primers targeting 18S rRNA genes of Cryptosporidium and piroplasms have identified Colpodella species in arthropods, host blood and feces, demonstrating the phylogenetic closeness of Colpodella species to the apicomplexa. However, in human, animal and arthropod hosts, the life cycle stages of Colpodella are unknown. In this literature review, we provide an overview of the widespread occurrence of Colpodella species in ticks, and pathogenicity in humans and animals. We discuss methods …


The Impact Of Narratives And Active Video Games Among Black And Hispanic Children With Overweight And Obesity: A Randomized Controlled Trial, Amy S Lu, Tom Baranowski, Tiago V Barreira, Amy Fleischman, Melanie C Green, Shirley Y Huang, I-Min Lee, Lynne L Levitsky, Farzad Noubary, Debbe Thompson May 2025

The Impact Of Narratives And Active Video Games Among Black And Hispanic Children With Overweight And Obesity: A Randomized Controlled Trial, Amy S Lu, Tom Baranowski, Tiago V Barreira, Amy Fleischman, Melanie C Green, Shirley Y Huang, I-Min Lee, Lynne L Levitsky, Farzad Noubary, Debbe Thompson

Children’s Nutrition Research Center Staff Publications

Background: Overweight and obesity disproportionately affect Black and Hispanic children who also play more video games. Narratives, coupled with home-based active video games (AVGs), may enhance PA and mitigate these disparities. This study tested the effect of narrative-enhanced home-based AVGs among predominantly Black and Hispanic children with overweight and obesity.

Methods: This 6-month three-group RCT recruited 135 children aged 7-14 from pediatric clinics in Boston, MA (January 2020 - May 2022) during the COVID-19 pandemic. Participants were randomized into: [Narrative + AVG], receiving an Xbox/Kinect with six AVGs interspersed with a narrative animation Ataraxia (72 episodes over six months), which …


Wildlife Road Mortalities During Covid-19 Pandemic-Related Lockdown In South Texas: A Comparative Survey, Bradley Evan Beer, Kevin W. Ryer, Md Saydur Rahman, John H. Young Jr., Richard J. Kline May 2025

Wildlife Road Mortalities During Covid-19 Pandemic-Related Lockdown In South Texas: A Comparative Survey, Bradley Evan Beer, Kevin W. Ryer, Md Saydur Rahman, John H. Young Jr., Richard J. Kline

School of Earth, Environmental, & Marine Sciences Faculty Publications

Mortalities of wildlife caused by collisions with vehicles along roads are increasing in prevalence, threatening the existence of various species and populations. The COVID-19 pandemic-related lockdown provided an opportunity to gain a better understanding of how wildlife vehicle mortality occurrences change in response to anthropogenic variables and how varying survey methods influence obtaining mortality data. In this study, data were collected in three observation periods: pre-lockdown (PreL), during lockdown (DL), and post-lockdown (PostL) in south Texas. There were 194 wildlife mortalities recorded during weeks 4–27 of 2020. Results of this study showed that road mortality survey counts did not change …


Ammonia Regulation In Aedes Taeniorhynchus, A Salt-Water Mosquito, Joseph Edmonds May 2025

Ammonia Regulation In Aedes Taeniorhynchus, A Salt-Water Mosquito, Joseph Edmonds

Undergraduate Honors Theses

The salt tolerant mosquito species Aedes taeniorhynchus has been observed in high salinity and ammonia conditions. Previous studies indicate Na+/K+ ATPase (NKA), the proton pump vacuolar-type H+ ATPase (VHA) and sodium-dependent cation-chloride cotransporters (NKCC’s) in sodium secretion in larval Aedes taeniorhynchus. However ammonia transporter such as Rh-protein, Amt1, and Amt2 have only been characterized in Aedes taeniorhynchus’s fresh water relative Aedes aegypti. Our research exhibits the expression of these transporters in A.taeniorhynchus using immunohistochemistry techniques. Evidence of VHA and NKA function in ammonia transport was characterized in vivo using drug antagonists in varying saline and ammonia concentrations. Ammonia flux rates …


Developing A Murine Model To Investigate The Mechanisms Of Peanut, Cashew, Shrimp Multifood Allergy, Aaron Wilson May 2025

Developing A Murine Model To Investigate The Mechanisms Of Peanut, Cashew, Shrimp Multifood Allergy, Aaron Wilson

NYMC Student Theses and Dissertations

Approximately 32 million people in the United States have food allergies, which is approximately 10 percent of the population. Food allergies can be mild, moderate, or even severe, resulting in anaphylaxis. The most predominant food groups that trigger food allergies include peanuts, tree nuts, fish, and shellfish. Peanuts and tree nuts have some similarities in protein structure. Shellfish is not associated with the protein properties of peanuts or tree nuts, it is the leading cause of adult anaphylaxis and often co-exist with peanut and tree nut allergies Currently, there are few studies that have established a murine model for allergy …


Targeting Neuronal Nitric Oxide Synthase (Nnos) As A Novel Approach To Enhancing The Anti-Melanoma Activity Of Immune Checkpoint Inhibitors, Anika R. Patel, Shirley Tong, Kate Alison Lozada, Amardeep Awasthi, Richard B. Silverman, Jennifer Totonchy, Sun Yang May 2025

Targeting Neuronal Nitric Oxide Synthase (Nnos) As A Novel Approach To Enhancing The Anti-Melanoma Activity Of Immune Checkpoint Inhibitors, Anika R. Patel, Shirley Tong, Kate Alison Lozada, Amardeep Awasthi, Richard B. Silverman, Jennifer Totonchy, Sun Yang

Pharmacy Faculty Articles and Research

Background and Objectives: Neuronal nitric oxide synthase (nNOS) overexpressed in melanoma plays a critical role in disease progression. Our previous studies demonstrated that nNOS inhibitors exhibited potent anti-melanoma activity and regulated PD-L1 expressions in the presence of interferon-gamma (IFN-γ). However, the role of nNOS in the melanoma immune response has not been well defined. Methods: Changes in gene expression profiles after nNOS inhibitor treatment were determined by transcriptomic analysis. A melanoma mouse model was used to determine the effects of nNOS inhibition on peripheral T cells and the in vivo anti-tumor activity of combining nNOS inhibitors with immune …


Thsd1 Is A Multifaceted Regulator In Health And Disease, Mengjun Dai, Kuizhi Qu, Sophie Liu, Zhen Xu, Yan-Ning Rui May 2025

Thsd1 Is A Multifaceted Regulator In Health And Disease, Mengjun Dai, Kuizhi Qu, Sophie Liu, Zhen Xu, Yan-Ning Rui

Faculty, Staff and Student Publications

Thrombospondin Type 1 Domain-Containing Protein 1 (THSD1) is a transmembrane protein increasingly recognized for its critical roles in vascular biology and disease pathogenesis. Initially identified as a marker of hematopoietic stem and endothelial cells during embryogenesis, THSD1 has since been implicated in a wide spectrum of physiological and pathological processes. This paper consolidates current knowledge on THSD1, with a focus on its roles in vascular integrity, perinatal disorders, and tumorigenesis. In vascular systems, THSD1 promotes focal adhesion assembly and suppresses autophagy-mediated adhesion turnover, thereby stabilizing endothelial attachment and maintaining barrier function. Genetic and functional studies support its protective role against …


More Than 9 Lives: Improving Shelter Cat Intake And Adoption Rates, Arthur S. Loomis May 2025

More Than 9 Lives: Improving Shelter Cat Intake And Adoption Rates, Arthur S. Loomis

Masters Theses

Municipal animal shelters across the United States struggle to properly care for the cats housed in their facilities due to overpopulation. The sheer volume of cats entering shelters greatly outpaces the rate in which they are adopted out, resulting in an immense resource strain that too often results in the euthanasia of millions of shelter cats across the country each year. The enormous influx of cats is largely due to abandonment or considerable populations of feral, stray, or community cats living on the streets. In addition, shelter cat adoptions occur at a much slower pace, with many cats spending months, …


Comparative Efficacy Of Combined Versus Singular Auditory Alpha-Theta Brainwave Entrainment And Hypnotherapy Modalities In Chronic Pain Management, Jennifer Nicole Leavitt May 2025

Comparative Efficacy Of Combined Versus Singular Auditory Alpha-Theta Brainwave Entrainment And Hypnotherapy Modalities In Chronic Pain Management, Jennifer Nicole Leavitt

Doctoral Dissertations and Projects

This experimental quantitative research, a randomized controlled trial, evaluated combined efficacy of auditory alpha-theta brainwave entrainment (BWE) and hypnotherapy in chronic pain management versus each modality alone. Chronic pain is a serious clinical challenge that may benefit from novel, nonpharmacological interventions. Previous studies have demonstrated benefits of both BWE and hypnotherapy for pain; but research on combined effects was not identified. This study hypothesized that a synergistic approach would offer superior pain relief compared to singular modalities. The study utilized a randomized controlled trial design with a sample size of 38 participants, initially, aged 25 to 65 years old, recruited …


Renal Patients’ Perception Of Self-Efficacy Through Rapport And Trust With Registered Dietitians, Michelle Denise Broussard May 2025

Renal Patients’ Perception Of Self-Efficacy Through Rapport And Trust With Registered Dietitians, Michelle Denise Broussard

Walden Dissertations and Doctoral Studies

End stage renal disease requires dietary education and behavior modifications to manage the condition. The purpose of this quantitative study was to investigate the role of rapport and trust between the patients and registered dietitians and its impact on self-efficacy. The study was implemented over 12 weeks in multi-site dialysis units in urban Georgia. The health belief model was used as the framework to explain the participants’ desire toward self-efficacy. Data were obtained for the 14-item Health Care Relationship Trust scale and the self-efficacy scales and analyzed using multiple linear regression. A convenience sample of 125 patients between the ages …


The Role Of Cephalic Mechanosensory Bristles In Flight Initiation Of The Oleander Hawkmoth, Daphnis Nerii, Maitri Manjunath May 2025

The Role Of Cephalic Mechanosensory Bristles In Flight Initiation Of The Oleander Hawkmoth, Daphnis Nerii, Maitri Manjunath

Theses and Dissertations

During flight, insects sense air movement past their bodies via sensory bristles distributed all over their body. These bristles provide mechanosensory feedback to their nervous system, thereby modulating wing motion to cope with ambient wind conditions. In many insect systems including locusts, moths, etc., flight can be rapidly initiated by stimulating a set of bristles called cephalic bristles on their head. Rapid initiation of flight requires diverse, distributed flight-related reflexes to be simultaneously activated.

Thus, we hypothesized that cephalic bristles directly and simultaneously trigger diverse flight-related reflexes. We tested this hypothesis using the Oleander hawkmoth, Daphnis nerii, as our study …


Effects Of Fibrogenic And Non Fibrogenic Nanomaterials On Cellular And Molecular Responses In Thp-1 Macrophage, Adedayo E. Oyerinde May 2025

Effects Of Fibrogenic And Non Fibrogenic Nanomaterials On Cellular And Molecular Responses In Thp-1 Macrophage, Adedayo E. Oyerinde

Biotechnology Theses

Traditional toxicological testing of nanomaterials is often low-throughput and time-consuming. Here, we aimed to develop an economically viable in vitro assay to prescreen nanomaterials with fibrogenic potential. To this end, we tested the generation of reactive oxygen species (ROS) as well as cytokines (pro- and anti-inflammatory) by macrophages following treatment with fibrogenic and non-fibrogenic nanomaterials. Both classes of nanomaterials induced ROS indistinguishably, suggesting that ROS production doesn’t predict nanomaterial fibrogenicity. We observed higher levels of IL1β and IL6 in macrophages treated with fibrogenic nanomaterials. Analysis of single-cell RNA-Sequencing data of fibrotic lungs from rodents and humans revealed overexpression of Brain …


Cost-Effective Solutions For High-Throughput Enzymatic Dna Methylation Sequencing, Amy Longtin, Marina M. Watowich, Baptiste Sadoughi, Rachel M. Petersen, Sarah F. Brosnan, Kenneth Buetow, Qiuyin Cai, Cayo Biobank Research Unit, Vanderbilt University, Michael D. Gurven, James P. Higham, Heather M. Highland, Yi-Ting Huang, Hillard Kaplan, Thomas S. Kraft, Yvonne A. L. Lim, Jirong Long, Amanda D. Melin, Michael J. Montagne, Jamie Roberson, Kee Seong Ng, Michael L. Platt, India A. Schneider-Crease, Jonathan Stieglitz, Benjamin C. Trumble, Vivek Venkataraman, Ian J. Wallace, Jie Wu, Noah Snyder-Mackler, Angela Jones, Alexander G. Bick, Amanda J. Lea May 2025

Cost-Effective Solutions For High-Throughput Enzymatic Dna Methylation Sequencing, Amy Longtin, Marina M. Watowich, Baptiste Sadoughi, Rachel M. Petersen, Sarah F. Brosnan, Kenneth Buetow, Qiuyin Cai, Cayo Biobank Research Unit, Vanderbilt University, Michael D. Gurven, James P. Higham, Heather M. Highland, Yi-Ting Huang, Hillard Kaplan, Thomas S. Kraft, Yvonne A. L. Lim, Jirong Long, Amanda D. Melin, Michael J. Montagne, Jamie Roberson, Kee Seong Ng, Michael L. Platt, India A. Schneider-Crease, Jonathan Stieglitz, Benjamin C. Trumble, Vivek Venkataraman, Ian J. Wallace, Jie Wu, Noah Snyder-Mackler, Angela Jones, Alexander G. Bick, Amanda J. Lea

ESI Publications

Characterizing DNA methylation patterns is important for addressing key questions in evolutionary biology, development, geroscience, and medical genomics. While costs are decreasing, whole-genome DNA methylation profiling remains prohibitively expensive for most population-scale studies, creating a need for cost-effective, reduced representation approaches (i.e., assays that rely on microarrays, enzyme digests, or sequence capture to target a subset of the genome). Most common whole genome and reduced representation techniques rely on bisulfite conversion, which can damage DNA resulting in DNA loss and sequencing biases. Enzymatic methyl sequencing (EM-seq) was recently proposed to overcome these issues, but thorough benchmarking of EM-seq combined with …


Understanding Host-Pathogen Interactions Using The Caenorhabditis Elegans – Orsay Virus Experimental Model, Chika Fujii May 2025

Understanding Host-Pathogen Interactions Using The Caenorhabditis Elegans – Orsay Virus Experimental Model, Chika Fujii

Arts & Sciences Graduate Student Theses and Dissertations

Over an evolutionary timeline, viruses have evolved ways to exploit biochemical pathways of the host for their propagation, whereas host organisms developed mechanisms to recognize virus infection and cope with them. Consequently, a better understanding of the different host factors involved in virus replication could provide insights into potential biological pathways or molecules for therapeutic targeting. Furthermore, detailed dissection of the mechanisms of virus sensing could reveal fundamentally important aspects of virus sensing that are evolutionarily conserved to mammals and offer insights into potentially underexplored areas of research. The C. elegans experimental model offers a useful platform for studying evolutionarily …


Multicomponent Parenteral Lipid Emulsions Do Not Prevent Liver Injury In Neonatal Pigs With Obstructive Cholestasis, Greg Guthrie, Caitlin Vonderohe, Valeria Meléndez Hebib, Barbara Stoll, Douglas Burrin May 2025

Multicomponent Parenteral Lipid Emulsions Do Not Prevent Liver Injury In Neonatal Pigs With Obstructive Cholestasis, Greg Guthrie, Caitlin Vonderohe, Valeria Meléndez Hebib, Barbara Stoll, Douglas Burrin

Children’s Nutrition Research Center Staff Publications

Biliary atresia (BA) is a pediatric liver disease that often necessitates parenteral nutrition (PN) to support growth due to impaired liver function. While soy-based lipid emulsions (SLE) are commonly used in PN, they may contribute to cholestatic liver injury. In contrast, mixed lipid emulsions (MLE) show promise in preventing cholestasis in infants without BA, potentially by restoring bile flow. However, their effectiveness in patients of complete bile duct obstruction, as seen in BA, remains uncertain. To explore the potential benefits of MLE in BA, we utilized a neonatal pig model of bile duct ligation (BDL). Pigs underwent either BDL or …


A Checklist Of Illinois Centipedes (Chilopoda), Gerald Summers, J. A. Beatty, Nanette Magnuson May 2025

A Checklist Of Illinois Centipedes (Chilopoda), Gerald Summers, J. A. Beatty, Nanette Magnuson

The Great Lakes Entomologist

Records for 45 centipede species occurring in Illinois are given with a brief discussion of general habitat features and the effects of certain environmental factors on specific distribution patterns. The chilopod fauna of Illinois includes species from southeastern, western, and northern physiographic regions and represents a richer mixture of chilopod species than is found in any adjacent state.


Description And Biology Of Bumblebees (Hymenoptera: Apidae) In Michigan, Robert W. Husband, Roland L. Fischer, T. Wayne Porter May 2025

Description And Biology Of Bumblebees (Hymenoptera: Apidae) In Michigan, Robert W. Husband, Roland L. Fischer, T. Wayne Porter

The Great Lakes Entomologist

The distribution of Michigan Bombinae was first studied by Milliron (1939). Records of Michigan bumblebees were included in Cockerell (1916) Franklin (1912). Lutz and Cockerell (1920). and Mitchell (1962). Other major studies in the Great Lakes Region were Chandler (1950) for Indiana, Medler and Carney (1%3) for Wisconsin, Husband (1966) for Michigan, Macfarlane (1974) for Ontario, LaBerge and Webb (1962) for Nebraska, Stevens (1948) for North Dakota, Frison (1926) for Illinois, and Neave (1933) for Manitoba. The purpose of this paper is to add to the information presented by Milliron (1939) and to complement the works by Chandler, Medler and …


Observations On The Biology Of The Slender Seedcorn Beetle, Clivina Impressifrons (Coleoptera: Carabidae), Robert D. Pausch, Lois M. Pausch May 2025

Observations On The Biology Of The Slender Seedcorn Beetle, Clivina Impressifrons (Coleoptera: Carabidae), Robert D. Pausch, Lois M. Pausch

The Great Lakes Entomologist

The slender seedcorn beetle, Clirina impressijirons, is known to attack germinating corn seed, but field and laboratory studies of its life history indicate it may sometimes play a beneficial role. Feeding studies show that adults and larvae are primarily carnivorous but will feed on corn seed when their primary food is absent or in short supply. Certain behavioral aspects indicate that populations of this beetle might be attracted to selected agricultural areas where adults and larvae could serve as predators of soil pests of corn. Much of the activity of these beetles is governed by temperature. with low temperatures below …


The Unicorn And Christ – Metamorphosis And Symbology, Luis Mendonça De Carvalho, Francisca Maria Fernandes, Maria Fátima Nunes, Ana Maria Costa, Paula Nozes, Ana Paula Figueira May 2025

The Unicorn And Christ – Metamorphosis And Symbology, Luis Mendonça De Carvalho, Francisca Maria Fernandes, Maria Fátima Nunes, Ana Maria Costa, Paula Nozes, Ana Paula Figueira

International Journal of Religious Tourism and Pilgrimage

The unicorn is a legendary chimaera that, for centuries, has been present in the mindset of Euro-Mediterranean cultures. Although its morphological description changed, tradition identifies it as an animal like a horse, albeit smaller and with a spiralling horn on its head pointing forward. The unicorn has been known since Antiquity, with references by authors such as Ctesias and Pliny. However, its relevance for medieval Europeans began with the Biblical references to this animal in several Old Testament verses. These verses gave legitimacy to the unicorn, and it was accepted as a tangible animal. During the Middle Ages and early …


Comparing Performance Of Aquaponics Systems Using Different Designs And Fish Feeds, Cody G. Willmore May 2025

Comparing Performance Of Aquaponics Systems Using Different Designs And Fish Feeds, Cody G. Willmore

Biology Theses, Dissertations, and Student Creative Activity

Future food production requires sustainable approaches that used both land and water resources efficiently, while remaining economically viable. One approach is aquaponics, the practice which combines hydroponics and aquaculture, as a sustainable food producing system. Aquaponic systems are unique, as the system can be designed to reuse and repurpose locally sourced materials and resources to meet individual needs. However, productivity of these systems can be inefficient or inconsistent, impacting the viability of aquaponics. The first objective of my study was to compare how system design between a do-it yourself reclaimed-materials aquaponic system and a prefabricated aquaponic setup affects water quality …


In Vivo Crispr Activation Screen Identifies Acyl-Coa-Binding Protein As A Driver Of Bone Metastasis, Hongqi Teng, Qinglei Hang, Caishang Zheng, Yuelong Yan, Shaomin Liu, Yang Zhao, Yalan Deng, Litong Nie, Weiche Wu, Marisela Sheldon, Zachary Yu, Wei Shi, Jianxuan Gao, Chenling Meng, Consuelo Martinez, Jie Zhang, Fan Yao, Yutong Sun, Di Zhao, Boyi Gan, Tong Meng, Li Ma May 2025

In Vivo Crispr Activation Screen Identifies Acyl-Coa-Binding Protein As A Driver Of Bone Metastasis, Hongqi Teng, Qinglei Hang, Caishang Zheng, Yuelong Yan, Shaomin Liu, Yang Zhao, Yalan Deng, Litong Nie, Weiche Wu, Marisela Sheldon, Zachary Yu, Wei Shi, Jianxuan Gao, Chenling Meng, Consuelo Martinez, Jie Zhang, Fan Yao, Yutong Sun, Di Zhao, Boyi Gan, Tong Meng, Li Ma

Faculty, Staff and Student Publications

One of the most common sites of cancer metastasis is to the bone. Bone metastasis is associated with substantial morbidity and mortality, and current therapeutic interventions remain largely palliative. Metastasizing tumor cells need to reprogram their metabolic states to adapt to the nutrient environment of distant organs; however, the role and translational relevance of lipid metabolism in bone metastasis remain unclear. Here, we used an in vivo CRISPR activation screening system coupled with positive selection to identify acyl-coenzyme A (CoA) binding protein (ACBP) as a bone metastasis driver. In nonmetastatic and weakly metastatic cancer cells, overexpression of wild-type ACBP, but …


Simultaneous Targeting Of Tumor Cells And Tumor-Associated Macrophages To Reprogram Glioblastoma Using Trypsinized Extracellular Vesicles Carrying Tumor Suppressive Microrna, Grace H Nguyen, Minhye Noh, Jin Muk Kang, Alexandra A Miller, Minxin Huang, Jiyeon Kim, Jeong-Yeon Lee, Sangwoon Chung, Hongyu Wang, George A Calin, Cynthia Ju, Holger K Eltzschig, Yeshavanth Banasavadi-Siddegowda, Zhongming Zhao, Ji Young Yoo, Tae Jin Lee May 2025

Simultaneous Targeting Of Tumor Cells And Tumor-Associated Macrophages To Reprogram Glioblastoma Using Trypsinized Extracellular Vesicles Carrying Tumor Suppressive Microrna, Grace H Nguyen, Minhye Noh, Jin Muk Kang, Alexandra A Miller, Minxin Huang, Jiyeon Kim, Jeong-Yeon Lee, Sangwoon Chung, Hongyu Wang, George A Calin, Cynthia Ju, Holger K Eltzschig, Yeshavanth Banasavadi-Siddegowda, Zhongming Zhao, Ji Young Yoo, Tae Jin Lee

Faculty, Staff and Student Publications

Glioblastoma (GBM) remains difficult to treat due to poor drug delivery across the blood-brain barrier and an immunosuppressive tumor microenvironment (TME). Tumor-suppressive microRNAs (miRNAs) offer a promising strategy to reprogram both tumor cells and the TME, but inefficient delivery systems limit their clinical application. We previously reported that tumor-suppressive miR-138 regresses tumor growth in preclinical GBM models. Here, we demonstrate that trypsin digestion of extracellular vesicles (EVs) enhances labeling efficiency with folate (FA), enhancing selective targeting of folate receptor (FR)-positive GBM cells and enabling simultaneous targeting of tumor-associated macrophages (TAMs). FA-labeled trypsinized EVs (tEVs) loaded with miR-138 inhibit tumor growth, …


Traceable And Scalable Food Balance Sheets From Agricultural Commodity Supply And Utilization Accounts (2010–2022), Xin Zhao, Maksym Chepeliev, Neus Escobar, Matthew T. Binsted, Pralit Patel, Page Kyle, Marshall A. Wise May 2025

Traceable And Scalable Food Balance Sheets From Agricultural Commodity Supply And Utilization Accounts (2010–2022), Xin Zhao, Maksym Chepeliev, Neus Escobar, Matthew T. Binsted, Pralit Patel, Page Kyle, Marshall A. Wise

Department of Agricultural Economics Faculty Publications

The Food Balance Sheets (FBS), compiled by the Food and Agriculture Organization (FAO), serve as a cornerstone dataset for studies on agricultural development, food security, and dietary health, providing a broad overview of global and regional food systems. However, its limited transparency and scalability hinder its application in empirical analysis and multisector dynamic modeling. Here, we present a traceable Food Balance Sheets (T-FBS) dataset, developed from detailed Supply Utilization Accounts (SUA) using a novel Primary Commodity equivalent (PCe) aggregation approach. This framework enables the aggregation of commodity flows along supply chains while ensuring consistency and balance across multiple dimensions. The …


Investigating The Role Of Lysophosphatidic Acid Receptor 3 In Ocular Surface Ion Transport And Fluid Secretion, Ethan Lindgren May 2025

Investigating The Role Of Lysophosphatidic Acid Receptor 3 In Ocular Surface Ion Transport And Fluid Secretion, Ethan Lindgren

Natural Sciences and Mathematics | Biological Sciences Master's Theses

Dry eye disease (DED) is a multifactorial disease that drives tear film instability and hyperosmolarity, directly damaging the ocular surface epithelium. Lysophosphatidic acid receptor (LPAR) is a G-protein coupled receptor (GPCR) responsible for Ca2+ and cAMP mobilization via PLC and adenylyl cyclase activation, respectively. LPAR signaling is involved in cell proliferation and wound healing in human corneal epithelial cells (HCECs) as well as in neuropathic pain. This study aims to investigate expression and functional roles of LPAR3 in ocular surface. Ocular surface potential difference (OSPD) and tear fluid volume measurements were performed in mice with topically applied LPAR modulators. …


Network Analysis Of Antimicrobial Resistance In Staphylococcus Aureus: Characterization Of Hub Genes And Their Functional Implications, Md Imran Hasan, Davida Smyth, Jeong Yang, Ashley Teufel May 2025

Network Analysis Of Antimicrobial Resistance In Staphylococcus Aureus: Characterization Of Hub Genes And Their Functional Implications, Md Imran Hasan, Davida Smyth, Jeong Yang, Ashley Teufel

Masters Theses (Archived)

Antimicrobial resistance is a major cause of morbidity and mortality in patients with S. aureus infections. In this study, we analyzed genes, molecular mechanisms, and pathways driving drug resistance in S. aureus using network analysis. Using whole-genome sequencing (WGS) data and systems biology approaches, we identified 229 AMR-associated genes and constructed a protein-protein interaction network among these genes. Through network topology and functional enrichment analyses, we not only confirmed their association with resistance, but also highlighted the central roles of these genes in resistance pathways, such as efflux, target replacement, and target protection, which are directly linked to multiple drug …


Evaluation Of Schoenoplectus Californicus In An Artificial System For E. Coli Removal, Kimia Ahmadiyehyazdi May 2025

Evaluation Of Schoenoplectus Californicus In An Artificial System For E. Coli Removal, Kimia Ahmadiyehyazdi

Masters Theses (Archived)

Green technologies and nature-based solutions like constructed wetlands have be come more popular due to their environmentally friendly approach to remediation, low cost of construction, and maintenance. Also, they have shown efficiency in removing a wide range of contaminants. However, choosing a suitable aquatic plant for this system is one of the questions that should be answered wisely. Schoenoplectus californicus– Giant Bulrush is an aquatic plant that was tested in this controlled constructed wetland, and the final concentration of the targeted microbial contamination, which is E. coli, was reduced remarkably. Also, for this study, soil was removed from the …


Honey Bee & Reward Loss: A Deep Dive Into The Alarm Pheromone, Citlalli Guadarrama May 2025

Honey Bee & Reward Loss: A Deep Dive Into The Alarm Pheromone, Citlalli Guadarrama

Masters Theses (Archived)

No abstract provided.