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Genetic Factors Of Campylobacter Jejuni Required For Its Interactions With Free-Living Amoeba, Deepti Pranay Samarth, Asim Z. Abbasi, Young Min Kwon

Poultry Science Faculty Publications and Presentations

Acanthamoeba, a free-living amoeba ubiquitous in environmental water, has been considered as the environmental reservoir of certain bacterial pathogens, including Campylobacter jejuni, an intracellular human pathogen causing self-limiting gastroenteritis. Acanthamoeba-C. jejuni interaction mechanisms may help clarify how the otherwise fastidious bacterium C. jejuni survives in environmental waters. In this study, we constructed single deletion mutants of C. jejuni strain 81-176 for the 10 selected genes (motAB, ciaB, kpsE, virB11, cheY, flaAB, cstII, docB, sodB, and cadF) previously shown to be important for the interaction (invasion and intracellular survival) of C. jejuni with mammalian hosts. We used a modified gentamicin protection …

Anti-Viral Cd8 Central Memory Veto Cells As A New Platform For Car T Cell Therapy, Wei-Hsin Liu, Anat Globerson Levin, Assaf Lask, Galit Horn, Tova Waks, Bar Nathansohn Levi, Irit Milman Krentsis, Einav Shoshan, Xiaohua Su, Maksim Mamonkin, Richard E Champlin, Yair Reisner, Esther Bachar Lustig

Faculty, Staff and Student Publications

Central memory CD8 T cells exhibit marked veto activity enhancing engraftment in several mouse models of T cell-depleted bone marrow (TDBM) allografting. Graft-versus-host disease (GVHD) can be prevented by stimulation of mouse or human memory CD8 T cells against their cognate antigens under cytokine deprivation, in the early phase of culture followed by further expansion with IL21, IL15, and IL7. Thus, human anti-viral CD8 central memory veto T cells generated from CMV and EBV-positive donors are currently evaluated in a clinical trial at MD Anderson Cancer Centre (MDACC). Results in 15 patients indicate a low risk of GVHD. Considering that …

Diversity And Activity Of Ground-Dwelling Spiders (Araneae) In Four Sub-Communities In A Degraded Oak Woodland At The Chicago Botanic Garden, Cook County, Illinois, James F. Steffen, Michael L. Draney

The Great Lakes Entomologist

Ground-dwelling spiders were sampled using pitfall traps over a twelve-month period from four sub-communities within a 40-ha oak woodland complex in northern Cook County, Illinois. Mesic and dry mesic oak woodland, mesic upland forest, buckthorn dominated mesic oak savanna and a mesic woodland control, where no restoration management is planned, were sampled. Fifty-five species within 44 genera and 18 families were trapped. The results suggest negative correlations between non-native spider abundance and spider species richness. Also, phenology of the various species indicates that a year-round inventory is necessary to adequately sample ground-dwelling spider assemblages. Possibilities of using Bathyphantes concolor (Wider) …

Habitat Distribution Of Carabid Beetles (Coleoptera: Carabidae) In Northern Hardwood Forests Of Michigan, Holly A. Petrillo, John A. Witter

The Great Lakes Entomologist

We compared carabid beetle communities in northern hardwood forests and divided these forests into three categories based on overstory composition and geographic location: Northern red oak-American beech (Quercus rubra L.-Fagus grandifolia Ehrh.) in the Lower Peninsula (OBLP), sugar maple- American beech (Acer saccharum Marsh.) in the Lower Peninsula (MBLP), and sugar maple- American beech in the Upper Peninsula (MBUP). Carabid beetles were collected using pitfall traps in 36 stands in 2003-2004. We found that carabid composition changed throughout the collection periods. Pterostichus pensylvanicus LeConte and Sphaeroderus lecontei Dejean comprised 76% of the trap catches in May while Synuchus impunctatus (Say) …

Spatiotemporal Variation Of Violet-Feeding Large Fritillaries (Euptoieta, Speyeria) (Lepidoptera: Nymphalidae) In Central And Northern Wisconsin, Ann B. Swengel, Scott R. Swengel

The Great Lakes Entomologist

We analyzed the relative abundance of the large fritillaries Euptoieta claudia (Cramer) (immigrant), Speyeria cybele (Fabricius) and S. atlantis (Edwards) (generalists), S. aphrodite (Fabricius) (grassland species), and S. idalia (Drury) (prairie specialist) in transect surveys at grasslands and pine barrens during 1990-2006 for variation and correlation over time (measured both as chronological year and as years since last management treatment) among six subregions in central and northern Wisconsin. Population trends (correlations of abundance with chronological year) were few and not significantly skewed toward either negative or positive trends. Spatial synchrony between pairs of subregions was significantly different among species, and …

The Native And Introduced Bark And Ambrosia Beetles Of Michigan (Coleoptera: Curculionidae, Scolytinae), Anthony I. Cognato, Nicolas Barc, Michael Philip, Robert Mech, Aaron D. Smith, Eric Galbraith, Andrew J. Storer, Lawrence R. Kirkendall

The Great Lakes Entomologist

Our knowledge of the biogeography of Scolytinae of eastern temperate North America is very patchy. We used data from hand collecting, trapped material (from 65 of 83 counties), and museum collections, supplemented by literature records, to compile a list comprising 107 bark beetle species in 45 genera for Michigan, a state with an especially rich diversity of woody plants. We provide detailed collection data documenting 32 species not previously catalogued for Michigan, 23 of which are new state records; the genera Trypophloeus and Trischidias are reported from Michigan for the first time. Fifteen Michigan scolytines are not native to North …

Simulation Of The Effects Of Stand Factors On Spruce Budworm (Lepidoptera: Tortricidae), W. P. Kemp, J. P. Nyrop, G. A. Simmons

The Great Lakes Entomologist

A model was developed to simulate the life history of instar-I through instar-I1 spruce budworm, Choristoneura fumiferana (Clemens). Particular emphasis was given to the redistribution phases during spring and fall and the factors affecting budworm survival. Analyses included the effects of aggregated stand types, temperature, cloud cover, open areas, and the species distribution, on redistribution mortality. Of those stand factors examined, open area and non-host percentages were the most important in regulating redistribution survival.

Size Reduction Southward In Michigan's Mustard White Butterfly, Pieris Napi (Lepidoptera: Pieridae), W. H. Wagner Jr., Michael K. Hansen

The Great Lakes Entomologist

[Excerpt]

The mustard white, Pieris napi (Linnaeus) is generally considered to be a circumboreal species or species-complex. Much disagreement exists regarding the classification in this group, the members of which are extremely diverse, especially in Eurasia. There is evidently "a continuous range of differentiation, from local populations, through subspecies, to species, which nomenclature cannot fully reflect" (Bowden 1972). In North America the major controversy has been whether the so-called West Virginia white, P. virginiensis Edwards, should be separated as a distinct species. Most writers today agree that it should. Of the mustard white in the Great Lakes area, the local …

An Annotated List Of The Checkered Beetles (Coleoptera: Cleridae) Of Michigan, D.C. L. Gosling

The Great Lakes Entomologist

[Excerpt]

The checkered beetles (Cleridae) have been included in local lists of Michigan Coleoptera by Andrews ( 1916, 192 1, 1929), Hatch (1924), Hubbard and Schwarz (1878). LeConte (1850), and Wolcott (1909). The present list is the first to include records from throughout the state, and is based on the collections of the University of Michigan Museum of Zoology (UMMZ), those of the Department of Entomology, Michigan State University (MSUC), and the private collections of E. F. Giesbert, D. K. Young, and the author. This list records 35 species of Cleridae from the state, and I believe it is essentially …

The Possible Cardioprotectiveeffect Of Empagliflozin During Endotoxemia In Mice Model, Safwa Miqdad Al-Killidar, Bashaer M. Muhammad-Baqir

Maaen Journal for Medical Sciences

Background:Sepsis is a major complication for seriously ill people with shock, major infections, serious burns, or severe trauma. Inflammation imbalance is the primary underlying cause of sepsis progression and persists continuously over the entire sepsis course. Empagliflozin is an orally administered medication for diabetes that selectively inhibits sodium-glucose cotransporter 2 to improve kidney elimination of glucose. It has been discovered that sodium-glucose cotransporter 2 exhibit anti inflammation and multiple effects, suggesting that they may be beneficial and prevent infections. Aim of study:This study was undertaken to assess Empagliflozin's cardioprotective efficacy in septic mice through its potent anti- inflammatory …

Seroprevalence And Associated Risk Factors Of Caprine Arthritis Encephalitis (Cae) And Peste Des Petits Ruminants (Ppr) Among Deer In An Institutional Farm In Malaysia, Krishnan Nair Balakrishnan, Faez Firdaus Abdullah Jesse, Bura Thlama Paul, Shalonagiftsal Stephen C Charles, Ummul Rifhan Najiha Noor Zaman, Eric Lim Teik Chung, Nur Amira Azhar, Abdirahman Barre, Muhammed Mikail, Mohd Azmi Mohd Lila

The Thai Journal of Veterinary Medicine

Caprine arthritis encephalitis (CAE) and peste des petits ruminants (PPR) are significant viral diseases impacting small ruminants, leading to substantial economic losses and posing a potential threat to livestock health. Although seroprevalence evidence of these viruses has been documented in ruminants, there is a lack of data regarding their presence among farmed deer in Malaysia. This preliminary study aimed to determine the seroprevalence and associated risk factors for CAEV and PPRV infections among deer in Malaysia. A total of ninety-two blood sera from an institutional deer farm were screened for CAEV and PPRV antibodies using commercially available indirect ELISA test …

A New Genus And New Synonyms For Liburnia Reducta Van Duzee, 1907 (Hemiptera: Auchenorrhyncha: Delphacoidea: Delphacidae), Charles R. Bartlett, Adam M. Wallner

Insecta Mundi

The delphacid species Liburnia reducta Van Duzee is transferred to the new genus Calkinodelphax Bartlett and Wallner (Hemiptera: Auchenorrhyncha: Delphacoidea: Delphacidae) and is designated the type species of this genus under the name Calkinodelphax reductus (Van Duzee), new combination. Calkinodelphax reductus is broadly distributed from Florida, the Antilles, Central America, and northern South America. Calkinodelphax reductus is the subjective senior synonym of Pissonotus striolus Osborn, Delphacodes cornuta Beamer, and Euides fasciatella auct. (nec. Osborn). A new lectotype is designated for Liburnia reducta Van Duzee. The female holotype and male allotype of Liburniella fasciatella Osborn were reviewed and found to …

Four Synonyms Of Tetraleurodes Mori (Quaintance, 1899) (Hemiptera: Sternorrhyncha: Aleyrodidae), Natalia Von Ellenrieder, Raymond J. Gill, Ian C. Stocks

Insecta Mundi

Examination of type specimens of Aleyrodes errans Bemis, 1904, A. dorseyi Kotinsky, 1907 (replacement name for A. quaintancei Bemis, 1904), and A. splendens Bemis, 1904 and comparison with a large series of specimens of Tetraleurodes mori (Quaintance, 1899) (Hemiptera: Sternorrhyncha: Aleyrodidae) confirms that the first four names represent new junior subjective synonyms of the latter species, since their types do not differ from it in any significant way and display the combination of diagnostic characters that identifies that species.

Resumen

El análisis de ejemplares tipo de Aleyrodes errans Bemis, 1904, A. dorseyi Kotinsky, 1907 (nombre de …

Predator Management With A Single Bait? Use Of Eradicat For The Simultaneous Management Of Feral Cats, Red Foxes, And Wild Dogs, Tracey L. Kreplins, David Algar

Biosecurity Research Articles

Context

In Western Australia, there are three invasive predators that require management for agriculture and biodiversity protection, feral cats, wild dogs, and red foxes. These three predators often coexist in the same locality, suggesting potential efficacy gains can be made via simultaneous control. While Western Australian native species have evolved a high tolerance to poison baiting (1080), invasive predators have not. Therefore, landscape-scale baiting is commonly used for predator management. Aims

Eradicat baits designed for feral cat control have also been known to be consumed and control wild dogs and foxes. In this trial, we aimed to evaluate Eradicat as …

Comparative Methylation Analyses Across Juglans Species To Investigate Epigenetic Contributions To Fungal Resistance, Keertana Chagari

Honors Scholar Theses

Juglans cinerea (butternut) is a critically threatened North American tree species experiencing severe declines due to the fungal pathogen Ophiognomonia clavigignentijuglandacearum. In contrast, its Asian relative, Juglans ailantifolia (Japanese walnut), shows natural resistance. To investigate the genomic and epigenetic factors underlying this difference, we constructed and analyzed high-quality, chromosome-level genome assemblies for both species from long-read sequencing data (J. ailantifolia: 527Mb; J. cinerea: 586Mb), focusing on transposable element (TE) content, DNA methylation patterns, and regulation of pathogen resistance genes (PRGs).

Repeat analysis revealed that J. cinerea has a slightly higher overall transposable element (TE) content, with …

The Influence Of Geographic Ranges, Climatic Niches And Temperature Fluctuations On Population Variability, Cleber Ten Canten, Lauren Holian, Tad Dallas

Faculty Publications

The existence of patterns in population dynamics across species geographic ranges and climatic niches is a pervasive idea in ecology. Population variability (i.e. temporal variability in population density) should hypothetically increase near range edges or niche limits because of less suitable environments in these areas, but the occurrence of such patterns remains largely unexplored. Further, fluctuations in temperature could pose demographic constraints on populations and also influence their variability. We used Breeding Bird Survey data to show that the population variability of 97 resident North American birds consistently increases towards their niche limits and in areas with more variable temperatures, …

Learning That Sticks: How Students Remember Invasive Species Through Different Learning Methods, Francesco L. Miniello

Honors College Theses

This study aimed to determine which learning methods are most effective at helping students learn about invasive species. Research through literature reviews showed that active learning methods can best help students learn and retain information regarding aspects of the natural environment. This includes hands-on activities such as participating in discussions, making observations, playing games, and interacting with objects like animals. The experiment tested different active and passive learning methods. This included watching presentations under passive learning or reviewing information online through active learning. Results showed that participants felt more confident about learning the material through active learning and were more …

Neovascular Pruning By Ido1 Inhibitors Can Potentiate Immunogenic Cytotoxicity Of Ischemia-Targeted Agents To Synergistically Enhance Anti-Pd-1 Responsiveness, Shih-Chun Shen, Souvik Dey, James B. Duhadaway, Erika Sutanto-Ward, Maurice T. Hampton, Serguei V. Kozlov, George C. Prendergast, Alexander J. Muller

Department of Pathology, Anatomy, and Cell Biology Faculty Papers

BACKGROUND: Strategies for deploying indoleamine 2,3-dioxygenase 1 (IDO1)-targeted therapies for use against cancer have focused on IDO1's role in promoting peripheral immune tolerance that shields tumors from effector T cells. However, preclinical investigation of both primary and metastatic tumor development in the lungs has uncovered a previously unappreciated role for IDO1 in directing a counterregulatory response to interferon (IFN)-γ that realigns the local inflammatory environment to promote tumor neovascularization. Understanding how to therapeutically leverage the ability of IDO1 inhibitors to subvert inflammatory neovascularization within the tumor microenvironment has potential ramifications for future clinical development of these compounds.

METHODS: Pulmonary metastases …

Russell’S Viper Envenomation: The Challenge Of Diagnosis, Jing-Hua Lin, Wang-Chou Sung, Jiunn-Wang Liao, Dong-Zong Hung

BioMedicine

Russell’s viper envenomation is rare in Taiwan, and its typical clinical presentations, including consumption coagulopathy, acute renal failure, haemolysis, and increased capillary permeability, have been reported in the literature as case reports or series. Here, we report a case with an atypical presentation, and suspected to be a Russell’s viper bite due to the distinct characteristics of the snake and some progressive clinical signs/symptoms. He returned to health successfully after the correct antivenom was administered, and envenomation was ultimately confirmed by venom detection in the patient’s serum and urine samples.

Cyclohexylamine, The Principal Metabolite Of Cyclamate, Contracts The Epididymal Vas Deferens Of Rats By Affecting Endogenous Catecholamine Release Via Postsynaptic Α1aand Presynaptic Α2-Adrenoceptors In A Calcium-Dependent Manner, Yuh-Fung Chen, Yu-Wen Wang, Huei-Yann Tsai

BioMedicine

Background: Cyclohexylamine (CHA) is a principal metabolite of cyclamate, which was once one of the most prominently consumed non-sugar sweeteners. Earlier studies suggested that long-term use of cyclamate might be carcinogenic and genotoxic; however, no consistent evidence supports an association between cyclamate and cancer risk. However, this issue remains interesting. Cyclamate can be metabolized to CHA by the intestinal bacteria in humans and some animals. Previous reports indicated CHA could induce atrophy of rat testes and affect rat fertility, as well as contract rat vas deferens. However, the contractile mechanisms of CHA on rat vas deferens remains poorly understood. This …

The Protective Role Of Astaxanthin Against Oxidative Stress And Inflammation In Peritoneal Dialysis Rats, Ratih Tri Kusuma Dewi, Bambang Purwanto, Brian Wasita, Vitri Widyaningsih

BioMedicine

Background: Prolonged exposure of peritoneal membrane to dialysate solution is known to cause fibrosis mediated by imbalanced inflammatory response and oxidative stress. In this context, proinflammatory cytokines including TGF-β and IL-1β, along with oxidative stress markers namely SOD and MDA, present in circulation, could serve as indicators of fibrosis. This phenomenon has the potential to interfere with the optimization of Chronic Kidney Disease (CKD) treatment. Other oral antioxidant supplements have been widely studied to prevent peritoneal damage, but investigations regarding Astaxanthin (AST) effect on fibrosis marked with proinflammatory cytokine are still limited.

Aims: This study aimed to investigate the protective …

Warming A Calf, Cheyenne Reid, Reganne K. Briggs, Melanie Heaton, Rebekah Esplin, David Secrist

All Current Publications

Calves born in the winter or spring are often exposed to harsh weather and environmental conditions. After birth, calves may become chilled due to cold temperatures or not being dried off promptly. A chilled calf may be experiencing hypothermia. This fact sheet explains the different types of hypothermia and methods for warming a calf until it can be safely returned outside.

Tube-Feeding A Calf, Cheyenne Reid, Reganne K. Briggs, Melanie Heaton, Rebekah Esplin, David Secrist

All Current Publications

Producers may need to tube-feed a newborn calf to provide milk if calves are unable to nurse or suck from the nipple bottle or when calves are sick and need electrolytes. This fact sheet outlines the materials required and the steps to successfully tube-feed a calf.

The Role Of G6pd Variants And 3d Genomic Structure In The Development Of Pulmonary Hypertension, Christina M. Signoretti Ph.D.

NYMC Student Theses and Dissertations

Pulmonary hypertension is an under-recognized global health epidemic which is estimated to affect 1% of the population. It is associated with sustained mean pulmonary artery pressure greater than 20 mmHg, pulmonary artery remodeling, smooth muscle and endothelial cell proliferation and subsequent increased right ventricle hypertrophy due to increased afterload. Glucose-6-phosphate dehydrogenase (G6PD) is one of the key enzymes in the pentose phosphate pathway and has been previously linked to the development of pulmonary hypertension. G6PD deficiency is the most common human enzymopathy which affects approximately 400 million people worldwide and is a result of agricultural and epidemiological evolution in different …

The Effects Of Morphine, Thc, And Lsd On Synaptic Plasticity Of The Ventral Tegmental Area, Michael Von Gunten

Theses and Dissertations

The brain is an intricate network of neurons that communicate via synapses. Synaptic plasticity, which is the ability to strengthen or weaken synaptic connections, plays a key role in learning, memory, and reward learning. Disruptions to synaptic plasticity play a central role in addiction, particularly in brain circuits involved in reward and motivation. The mesolimbic reward system, which includes the ventral tegmental area (VTA) and its dopaminergic projections, is a primary target of drugs of abuse, including opioids and cannabinoids. However, the physiological consequences of combined exposure to opioids and cannabinoids and the potential for psychedelics to reverse drug-induced neuroadaptations …

From Epidemics To Experimental Treatments: Amphibian Mass Mortality Events And A Potential Treatment For Chytridiomycosis, Daniel Callahan

Graduate Masters Theses

Worldwide, amphibian populations have experienced dramatic declines due to a variety of threats. Among them, disease has been particularly deleterious and continues to impact populations. In the United States and Canada, the three leading causes of amphibian mass mortality events are ranavirosis, chytridiomycosis, and severe perkinsea infection, all caused by infectious pathogens. While research and policy have helped populations recover from these diseases, more work needs to be done to develop practical and efficient treatments for these infections. Of these three diseases, chytridiomycosis has had the most devastating effects on amphibian populations globally. Caused by the chytrid fungi Batrachochytrium dendrobatidis …

Adaptation And Discovery In The Ethiopian Highlands: Testing Rules, Cryptic Shrews, And Patterns Of Montane Mammal Diversity, Evan W. Craig

Graduate Doctoral Dissertations

Montane landscapes of the Horn of Africa harbor exceptional but incompletely documented biodiversity. Focusing on rodents and shrews—the dominant but least studied of Ethiopia’s high-elevation fauna—this dissertation integrates repeat faunal surveys, genomic taxonomy, and phylogenetically informed modelling to clarify how small mammals distribute, diversify, and morphologically adapt across steep elevational gradients.

A century‐scale resurvey of Simien Mountains National Park (1927-2015) revealed significant upward range shifts for multiple species and a persistent mid-elevation richness peak, implicating warming temperatures as a driver of community reassembly. Museomics using genome-skimming and craniodental morphometrics subsequently uncovered a deeply divergent miniature shrew lineage within the Crocidura …

Mechanisms Of Placental Impairment And The Upregulation Of Fetuin-B During Maternal Undernourishment, Mia Camilliere

NYMC Student Theses and Dissertations

Proper nutrition is crucial during pregnancy to support fetal and placental development. Failure to meet these nutritional needs can result in adverse health outcomes for both mother and baby. Despite its high prevalence in underdeveloped countries, maternal undernourishment is a public health issue worldwide. There are several possible causes of maternal undernourishment, and these can occur alone or in combination, such as poor access to nutrition, hyperemesis gravidarum, and drug use. This condition can adversely affect placental development and function to the extent of placental insufficiency, perturbing the entire pregnancy. The placenta is responsible not only for hormonally and physically …

Inactivation Of Aerosolized Hepatitis A Viral Droplets On Food Contact Surfaces By Ultraviolet-Light-Emitting Diodes At 255 Nm And 279 Nm, Breanna Polen, Ankit Patras, Brahmaiah Pendyala, Doris H. D’Souza

Food and Animal Sciences Faculty Research

Hepatitis A viral outbreaks continue to occur. It can be transmitted through aerosolized droplets and thus can contaminate surfaces and the environment. Ultraviolet light emitting diode (UV-C LED) systems are used for inactivation of microbes, though research is needed to determine optimal doses for aerosolized HAV inactivation. This study evaluates the UV-C LED doses for the inactivation of aerosolized hepatitis A virus (HAV) deposited on stainless-steel and glass discs. HAV was aseptically deposited onto stainless-steel or glass discs (1.27 cm diameter) using a nebulizer within a chamber followed by treatments for up to 1.5 min with 255 nm (surface dose …

Heterozygous Kmt2d Loss Diminishes Enhancers To Render Medulloblastoma Cells Vulnerable To Combinatory Inhibition Of Lsd1 And Oxphos, Shilpa S Dhar, Calena Brown, Ali Rizvi, Lauren Reed, Sivareddy Kotla, Constantin Zod, Janak Abraham, Jun-Ichi Abe, Veena Rajaram, Kaifu Chen, Min Gyu Lee

Faculty, Staff and Student Publications

The histone H3 lysine 4 (H3K4) methyltransferase KMT2D (also called MLL4) is one of the most frequently mutated epigenetic modifiers in many cancers, including medulloblastoma (MB). Notably, heterozygous KMT2D loss frequently occurs in MB and other cancers. However, its oncogenic role remains largely uncharacterized. Here, we show that heterozygous Kmt2d loss in murine cerebellar regions promotes MB genesis driven by heterozygous loss of the MB-suppressor gene Ptch via the upregulation of tumor-promoting programs (e.g., oxidative phosphorylation [OXPHOS]). Downregulation of the transcription-repressive tumor suppressor NCOR2 by heterozygous Kmt2d loss, along with Ptch