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Full-Text Articles in Entire DC Network
Effect Of Potassium Fertilizer On The Nutrition And Physical Quality Of Job’S Tears (Coix Lacryma-Jobi L.) Seeds, Qinthara Nail Haysa, Yuyun Yuwariah, Fiky Yulianto Wicaksono
Effect Of Potassium Fertilizer On The Nutrition And Physical Quality Of Job’S Tears (Coix Lacryma-Jobi L.) Seeds, Qinthara Nail Haysa, Yuyun Yuwariah, Fiky Yulianto Wicaksono
Jurnal Kultivasi
Job's tears is a cereal crop that has beneficial nutrients. Potassium is known to affect the growth and yield of cereal crops, but there has been limited study on whether potassium affects the nutrition and physical quality of Job’s tears seeds. This study aimed to analyze the effect of K fertilizer on the nutritional content and physical quality of Job's tears seeds. This research was conducted from January to May 2024 at Ciparanje Experimental Field, Faculty of Agriculture, Universitas Padjadjaran, Sumedang, Indonesia. This experiment used the Randomized Block Design (RBD) method with six treatments and four replications. Various doses of …
Polo-Like Kinase 1 Inactivation Enhances Pi3k Inhibition-Mediated Apoptosis Of Notch1-Mutant Head And Neck Squamous Cell Carcinoma, Pooja A Shah, Tuhina Mazumdar, Soma Ghosh, Lacin Yapindi, Reid T Powell, Yong S Park, Li Shen, Anne M Fernandez, Clifford C Stephan, Jing Wang, Andrew G Sikora, Jawad Kazi, Mitchell J Frederick, Faye M Johnson
Polo-Like Kinase 1 Inactivation Enhances Pi3k Inhibition-Mediated Apoptosis Of Notch1-Mutant Head And Neck Squamous Cell Carcinoma, Pooja A Shah, Tuhina Mazumdar, Soma Ghosh, Lacin Yapindi, Reid T Powell, Yong S Park, Li Shen, Anne M Fernandez, Clifford C Stephan, Jing Wang, Andrew G Sikora, Jawad Kazi, Mitchell J Frederick, Faye M Johnson
Children’s Nutrition Research Center Staff Publications
PI3K inhibition causes apoptosis selectively in NOTCH1-mutant head and neck squamous cell carcinoma (HNSCC), but modest single-agent responses and acquired resistance (AR) limit the clinical efficacy of targeted agents. To address these limitations, we investigated novel combination therapies. We tested the efficacy of 5768 compounds as single agents and 139 in combination with PI3K inhibitors in sensitive and AR NOTCH1-mutant HNSCC cell lines. We generated synergy/efficacy classifications for the combinations using multiple metrics of statistical drug synergy and growth rate indices. The PLK1/PI3K combination's efficacy was validated using orthogonal in vitro methods and in two HNSCC xenograft models. Compound efficacy …
Mutant P53 Confers Chemoresistance By Activating Kmt5b-Mediated Dna Repair Pathway In Nasopharyngeal Carcinoma, Haidan Luo, Mo-Fan Huang, An Xu, Donghui Wang, Julian A Gingold, Jian Tu, Ruoyu Wang, Zijun Huo, Yen-Ting Chiang, Kuang-Lei Tsai, Jie Su, Danielle A Bazer, Mien-Chie Hung, Canmao Xie, Yubiao Guo, Dung-Fang Lee, Huiling Yang, Ruiying Zhao
Mutant P53 Confers Chemoresistance By Activating Kmt5b-Mediated Dna Repair Pathway In Nasopharyngeal Carcinoma, Haidan Luo, Mo-Fan Huang, An Xu, Donghui Wang, Julian A Gingold, Jian Tu, Ruoyu Wang, Zijun Huo, Yen-Ting Chiang, Kuang-Lei Tsai, Jie Su, Danielle A Bazer, Mien-Chie Hung, Canmao Xie, Yubiao Guo, Dung-Fang Lee, Huiling Yang, Ruiying Zhao
Faculty, Staff and Student Publications
Nasopharyngeal carcinoma (NPC), a malignancy arising from the nasopharyngeal epithelium, is common in the east and southeast area of Asia. Treatments for locally advanced and recurrent NPC include chemotherapy (usually combined with 5-Fluorouracil, 5-FU) and radiotherapy, but response is limited due to chemo-resistance. p53 mutation is a critical factor for 5-FU resistance in some cancers, but its role in NPC chemo-resistance remains unclear. Here, we demonstrate that p53(R280T), a common p53 somatic mutation found in multiple NPC tumor samples, induces gain-of-function upregulation of DNA repair genes which leads to 5-FU resistance in NPC. p53(R280T) specifically upregulates the expression of DNA …
Shimmering Mirage Or A Promised Land? Climate Change, Agriculture, And Internal Migration In Sabha, Jordan, Tyler J. Rak
Shimmering Mirage Or A Promised Land? Climate Change, Agriculture, And Internal Migration In Sabha, Jordan, Tyler J. Rak
Capstone Collection
Given the rapidly shifting climate, high vulnerability, and low adaptability to climate related changes that rural areas face, this study highlights the complex relationship between climate change -- especially water scarcity -- agriculture, and internal migration in rural Jordan. I predict that if water scarcity is present and climate changes are occurring, they will undercut the rural agriculture sector which could contribute to rural out-migration. To study this relationship, I conducted a case study in Sabha, a small rural community in the Northern Badia of Jordan. Data was gathered by engaging in semi-structured interviews with current community members in Sabha …
From Larvae To Livelihoods: Examining The Realities Of Black Soldier Fly Farming In Malawi, Emily Herriott
From Larvae To Livelihoods: Examining The Realities Of Black Soldier Fly Farming In Malawi, Emily Herriott
Capstone Collection
Black Soldier Fly (BSF) larvae offer a cost-effective, nutrient-rich alternative to synthetic fertilizers and conventional feed inputs such as soy, fishmeal, and maize bran in sub-Saharan Africa. In Malawi, where smallholder farmers grapple with declining soil fertility, high input prices, chronic food insecurity, and livestock nutritional challenges, BSF farming is emerging as a promising circular agricultural innovation. Despite its technical promise, the social, economic, and ecological realities of BSF adoption remain underexplored.This study investigates the early implementation of BSF farming in Malawi through the Sustainable Livelihoods Framework, focusing on farmer experiences, challenges, and the social and structural processes shaping uptake …
Dairy And Food Science Student Newsletter, August 8, 2025, Department Of Dairy And Food Science
Dairy And Food Science Student Newsletter, August 8, 2025, Department Of Dairy And Food Science
Dairy and Food Science Student Newsletter
No abstract provided.
Northern Arizona Ecological Conservation: Assessing Quaking Aspen Health In Northern Arizona Using Earth Observations, Ikram Morso, Luke Collins, Margaret Cox, Melissa Schwan
Northern Arizona Ecological Conservation: Assessing Quaking Aspen Health In Northern Arizona Using Earth Observations, Ikram Morso, Luke Collins, Margaret Cox, Melissa Schwan
Aspen Bibliography
Quaking aspen (Populus tremuloides) forests in Northern Arizona provide critical habitat for endangered bird species and support regional tourism and recreation. However, aspen populations are declining due to a combination of abiotic and biotic stressors. In response, land managers implemented strategies, such as ungulate exclusion fencing, prescribed burns, and stand thinning, but efforts are constrained by limited data on where and when to intervene. This project worked in collaboration with the U.S. Forest Service, the National Park Service, the Arizona Department of Forestry and Fire Management, and Northern Arizona University School of Forestry. The team utilized Earth observations …
Chelidonium Majus L.-Containing Gel May Improve Diabetic Wound Healing By Modulating Mmp-2, Mmp-9, And Collagen Levels, Kaan Kaltalioğlu, Eli̇f Naz Gürsoy, Barbaros Balabanli, Zeki̇ Aytaç, Şule Coşkun Cevher
Chelidonium Majus L.-Containing Gel May Improve Diabetic Wound Healing By Modulating Mmp-2, Mmp-9, And Collagen Levels, Kaan Kaltalioğlu, Eli̇f Naz Gürsoy, Barbaros Balabanli, Zeki̇ Aytaç, Şule Coşkun Cevher
Turkish Journal of Biology
Background/aim: Delayed wound healing in diabetic patients is a significant complication that reduces quality of life, prompting the continuous investigation of new therapeutic agents. This study designed to explore the dose-dependent effects of different parts of Chelidonium majus L. (CM), a medicinal plant traditionally used for skin disorders, on diabetic skin wounds.
Materials and methods: In diabetic rats, full-thickness excisional wounds were formed. CM-containing gels (aerial parts at 3%, 6%, 9% and root at 0.5%, 1%, 1.5%) were developed and applied to the wounds. After the treatment period, the rats were sacrificed, and wound healing activity was assessed macroscopically, histopathologically, …
Investigating Food Web Dynamics Of Great Lakes Coastal Wetlands Using A Novel Stable Isotope Approach, Alyssa M. Smith
Investigating Food Web Dynamics Of Great Lakes Coastal Wetlands Using A Novel Stable Isotope Approach, Alyssa M. Smith
Masters Theses
Coastal wetlands of the Great Lakes support abundant populations of fish, invertebrates, and vegetation, though the trophic linkages connecting primary production and lower consumers is not well understood in these systems. We implemented a multiple-tracer approach, pairing traditional food web isotope tracers like carbon (δ13C) and nitrogen (δ15N) with total mercury concentrations (THg). We predicted that filamentous algae would be the dominant energy resource in the diet of lower trophic-level invertebrates in the Grand River Estuary, a network of riverine coastal wetlands adjacent to lake Michigan. In addition, we predicted that adding THg as a tracer would improve the resolution …
Different Hypoxic Response Of Human Cavii And Caenorhabditis Elegans Homologous Genes Cah-3, Cah-4, Cah-5, Sümeyye Aydoğan Türkoğlu, Aysu Bozkurt, Fatma Poyrazli, Derya Okuyan
Different Hypoxic Response Of Human Cavii And Caenorhabditis Elegans Homologous Genes Cah-3, Cah-4, Cah-5, Sümeyye Aydoğan Türkoğlu, Aysu Bozkurt, Fatma Poyrazli, Derya Okuyan
Turkish Journal of Biology
Background/aim: A number of carbonic anhydrase (CA) family proteins have been implicated in cancer. They contribute to the hypoxic microenvironment. CAVII is often downregulated in colorectal carcinoma and it has been associated with increased tumor size, node metastasis, and adverse clinical outcomes. In this study, we aimed to investigate the effect of hypoxia on CAVII protein in human colon cancer and prostate cancer cells. In addition, the regulation of CAH genes in Caenorhabditis elegans was examined. These are homologous to CAVII in humans.
Materials and methods: CAVII expression was analyzed in different cell lines such as human colon cancer (SW480 …
Mir-9-5p Alleviates The Development Of Abdominal Aortic Aneurysm By Regulating The Differentiation Of Cd4+Il-10+T Cells Via Targeting The Crosstalk Between Nrf2 And Nf-Κb Signaling Pathways, Hongfu Liu, Jinyi Zhang, Lubin Li, Benxiang Yu, Chunlei Zhang, Wenqiang Niu, Yawen Cheng, Hengyang Dong, Yukun Zhang, Xinlin Luo, Yanlian Xiong, Yueming Wang
Mir-9-5p Alleviates The Development Of Abdominal Aortic Aneurysm By Regulating The Differentiation Of Cd4+Il-10+T Cells Via Targeting The Crosstalk Between Nrf2 And Nf-Κb Signaling Pathways, Hongfu Liu, Jinyi Zhang, Lubin Li, Benxiang Yu, Chunlei Zhang, Wenqiang Niu, Yawen Cheng, Hengyang Dong, Yukun Zhang, Xinlin Luo, Yanlian Xiong, Yueming Wang
Turkish Journal of Biology
Background: Abdominal aortic aneurysm (AAA), a gradual segmental dilatation of the abdominal aorta, is associated with a high mortality rate. The pathophysiological molecular mechanisms underlying AAA remain unclear. In recent years, changes in miRNA levels have been reported to be involved in the development and treatment of AAA. This study aimed to investigate the potential targets and underlying mechanisms of miR-9-5p in attenuating AAA progression by modulating the inflammatory response.
Materials and methods: Biochemical kits were used to measure the levels of inflammatory factors, antioxidant enzyme activity, and serum oxidative stress in normal and AAA model mice. miR-9-5p overexpression was …
Lactic Acid Bacteria Alleviate Neuronal Excitotoxicity And Extend Caenorhabditis Elegans Lifespan, Bao Le, Thi Nhat Hong Nguyen, Seung-Hwan Yang
Lactic Acid Bacteria Alleviate Neuronal Excitotoxicity And Extend Caenorhabditis Elegans Lifespan, Bao Le, Thi Nhat Hong Nguyen, Seung-Hwan Yang
Turkish Journal of Biology
Aim: Neuronal cell death plays a critical role in the development of neurological disorders associated with aging. This study aimed to evaluate the beneficial effects of heat-killed lactic acid bacteria (hkLAB) on neuroblastoma cells and Caenorhabditis elegans.
Materials and methods: We pretreated heat-killed Lactobacillus fermentum CNU384 (hkCNU384), L. brevis CNU386 (hkCNU386), L. helveticus CNU395 (hkCNU395), and L. paracasei CNU396 (hkCNU396) at a concentration of 109 CFU/mL to investigate their neuroprotective effects on glutamate-induced SH-SY5Y cells and their impact on the lifespan of 6-hydroxydopamine (6-OHDA)-induced C. elegans.
Results: Our findings indicate that hkCNU395 and hkCNU396 protected against glutamate-induced cell death …
Recreational Boat Fishing Participation Varies Across Bioregional Fisheries In Western Australia, With Persistent Patterns Within Fisheries And Fisher Typologies Over Time, Karina L. Ryan, Eva Lai, Claire B. Smallwood, Jeremy M. Lyle, Kate E. Stark, Sean R. Tracey
Recreational Boat Fishing Participation Varies Across Bioregional Fisheries In Western Australia, With Persistent Patterns Within Fisheries And Fisher Typologies Over Time, Karina L. Ryan, Eva Lai, Claire B. Smallwood, Jeremy M. Lyle, Kate E. Stark, Sean R. Tracey
Fisheries Research Articles
Recreational fishing is popular worldwide and understanding variability in participation can inform policies that seek to improve sustain[1]ability of recreational fisheries and amenity for recreational fishers. However, not all recreational fishers are alike in their demographics or behaviours. This study explores temporal and spatial variability in licensed boat-based recreational fishing participation in Western Australia using data from a consistent survey design delivered 12 times from 2011 to 2024. Five fisher participation typologies were identified across four marine bioregions (North Coast, Gascoyne Coast, West Coast, and South Coast) and four fishery types (demersal, nearshore, pelagic, and invertebrate) with varied fisher characteristics …
Homologous And Heterologous Vaccination Regimens With Mrna And Rvsv Platforms Induce Potent Immune Responses Against Sftsv Glycoprotein, Tomaz B. Manzoni, Jonna B. Westover, Kendall A. Lundgreen, Philip D. Hicks, Raegan J. Petch, Jordan T. Ort, Drew Weissman, Steven H. Y. Fan, Scott E. Hensley, Norbert Pardi, Brian B. Gowen, Paul Bates
Homologous And Heterologous Vaccination Regimens With Mrna And Rvsv Platforms Induce Potent Immune Responses Against Sftsv Glycoprotein, Tomaz B. Manzoni, Jonna B. Westover, Kendall A. Lundgreen, Philip D. Hicks, Raegan J. Petch, Jordan T. Ort, Drew Weissman, Steven H. Y. Fan, Scott E. Hensley, Norbert Pardi, Brian B. Gowen, Paul Bates
Animal, Dairy, and Veterinary Science Faculty Publications
Background: Severe fever with thrombocytopenia syndrome virus (SFTSV) is a highly pathogenic bunyavirus with a high case-fatality ratio for which there is no approved vaccine. Studies have assessed different vaccine technologies. However, few studies have yet assessed the immunogenicity of heterologous prime-boost regimens. Methods: Here, we compare a lipid nanoparticle (LNP)-encapsulated nucleoside-modified mRNA-based vaccine encoding the SFTSV glycoproteins, Gn and Gc, to our recently described recombinant VSV SFTSV (rVSV-SFTSV) vaccine in single dose, homologous, and heterologous prime-boost regimens in mice. Results: We show that all regimens protect from pathogenic SFTSV challenge and elicit strong long-lasting antibody responses. Furthermore, strong cellular …
Post-Drought Growth Recovery Of Tea (Camellia Sinensis) Under Different Techniques And Doses Of Biofertilizer Applications, Nabila Ragil Wiharti, Intan Ratna Dewi Anjarsari, Yudithia Maxiselly
Post-Drought Growth Recovery Of Tea (Camellia Sinensis) Under Different Techniques And Doses Of Biofertilizer Applications, Nabila Ragil Wiharti, Intan Ratna Dewi Anjarsari, Yudithia Maxiselly
Jurnal Kultivasi
Drought stress is a major limiting factor affecting the growth, yield, and quality of tea (Camellia sinensis). The present study aimed to analyze the success of post-drought growth recovery of tea in response to different techniques and doses of biofertilizer applications. Field experiments were conducted from January to August 2024 in the experimental garden of the Research Institute for Tea and Cinchona, Gambung Blok A8, Bandung. This work was arranged in a split-plot design, consisting of a main plot with two biofertilizer application techniques (foliar feeding and soil drenching) and subplots with four levels of biofertilizer dosage (control (B1), 15 …
Efficacy Of Lactobacillus Rhamnosus And Its Metabolites To Mitigate The Risk Of Foodborne Pathogens In Hydroponic Nutrient Solution, Esther Oginni, Robin A. Choudhury, Veerachandra Yemmireddy
Efficacy Of Lactobacillus Rhamnosus And Its Metabolites To Mitigate The Risk Of Foodborne Pathogens In Hydroponic Nutrient Solution, Esther Oginni, Robin A. Choudhury, Veerachandra Yemmireddy
School of Earth, Environmental, & Marine Sciences Faculty Publications
Hydroponic nutrient solution (HNS) has been established as an ideal conduit for pathogen contamination and proliferation. This study evaluated the efficacy of lactic acid bacteria and their metabolites in mitigating the risk of foodborne pathogens in HNS when compared to conventional chemical treatments. Hoagland’s HNS were prepared according to the manufacturer’s instructions and inoculated with Salmonella Typhimurium, Escherichia coli 0157:H7, and Listeria innocua at 105 CFU/mL cell concentration. These nutrient solutions were subjected to treatment with various concentrations of Lactobacillus rhamnosus live cells, a cell-free extract (CFE) of L. rhamnosus metabolites, sodium hypochlorite and peroxyacetic acid at 22 ± 1 …
Abundance, Distribution, And Shoaling Of Driftfishes And Allies (Scombriformes: Stromateoidei) In The Pelagic Gulf Of Mexico, Juliet Tretler
Abundance, Distribution, And Shoaling Of Driftfishes And Allies (Scombriformes: Stromateoidei) In The Pelagic Gulf Of Mexico, Juliet Tretler
All HCAS Student Capstones, Theses, and Dissertations
The suborder Stromateoidei contains 80 species of mesopelagic fishes in five families distributed throughout temperate and tropical seas worldwide, including the Gulf of Mexico (Gulf hereafter). Mesopelagic fishes, especially those that spatially aggregate, serve as important forage items for commercially, recreationally, and ecologically important species of fish, marine mammals, and seabirds. However, knowledge of the ecology and trophic importance of stromateoid fishes in the Gulf is extremely limited. This study provides quantitative information on the species composition, abundance, distribution, and biomass contribution of the stromateoid species to the Gulf deep-pelagic fish assemblage, with comparison to “archetypal” mesopelagic fish families (e.g., …
Genetic And Environmental Factors Contributing To The Pathogenesis Of Vertebral Osteomyelitis Caused By Enterococcus Cecorum In Broiler Chicken, Ummu Syauqah Al Musyahadah, Andi Asnayanti, Anh Dang Trieu Do, Adnan Alrubaye
Genetic And Environmental Factors Contributing To The Pathogenesis Of Vertebral Osteomyelitis Caused By Enterococcus Cecorum In Broiler Chicken, Ummu Syauqah Al Musyahadah, Andi Asnayanti, Anh Dang Trieu Do, Adnan Alrubaye
Poultry Science Faculty Publications and Presentations
Enterococcus cecorum (EC) is the primary cause of Vertebral Osteomyelitis (VO) in poultry, characterized by a hock-sitting posture with legs extended forward. The disease begins with intestinal colonization, where pathogenic strains of E. cecorum establish themselves in the gut within the first week of a bird's lifecycle and translocate to the bloodstream later, reaching the organs, which finally infect the birds' Free Thoracic Vertebrae (FTV). Several factors contribute to the initiation and progression of the disease. Environmental factors such as heat stress, high blood sugar levels, prior infections, overcrowding, and rapid growth conditions weaken immunity and stimulate the emergence of …
The Effect Of Feed And Water Provision Strategies On Broiler Breeder Pullet Performance And Welfare, Allison D. Weaver, Lisa R. Bielke, Ramon D. Malheiros, Sara K. Orlowski, Allison N. Pullin
The Effect Of Feed And Water Provision Strategies On Broiler Breeder Pullet Performance And Welfare, Allison D. Weaver, Lisa R. Bielke, Ramon D. Malheiros, Sara K. Orlowski, Allison N. Pullin
Poultry Science Faculty Publications and Presentations
Feed restriction is common in the broiler breeder industry to optimize health and reproduction. However, this practice has been associated with increased drinking behavior, leading to water spillage, higher litter moisture, and footpad lesions. Consequently, parts of the industry have adopted water restriction protocols. This study aimed to evaluate how different combinations of feed and water restriction affected drinking behavior, welfare, and performance indicators in broiler breeder pullets. At 1 day of age, 960 Cobb 500 FF pullets (Gallus gallus domesticus) were randomly allocated to one of four treatments: skip-a-day feeding with ad libitum water (SAD + ADLIB), every-day feeding …
The Distribution Of Some Soil Arthropods In A Manipulated Ecosystem, James H. Shaddy, James W. Butcher
The Distribution Of Some Soil Arthropods In A Manipulated Ecosystem, James H. Shaddy, James W. Butcher
The Great Lakes Entomologist
[Excerpt] Soil cultivation practices and agricultural utilization affect soil animal populations in various ways. Gisin (1955) and Schalk (1968) concluded that the effect was minimal. Others observed a deleterious effect (Karg, 1956; Sheals, 1956; Strebel, 1957; Aleinikova and Utrobina, 1975; Edwards and Lofty, 1975). However, some soil animals appear to be benefited (Aleinikova and Utrobina, 1975; Edwards and Lofty, 1975). The effects of cultivation and many other factors have been discussed thoroughly in reviews by Christiansen (1964), Burges and Raw (1967), Butcher, et al. (1971), Ghilarov (1975), and Wallwork (1976).
A Distinctive Dune Form Of The Marbled White Butterfly, Euchloe Olympia (Lepidoptera: Pieridae) In The Great Lakes Area, Warren Herb Wagner Jr.
A Distinctive Dune Form Of The Marbled White Butterfly, Euchloe Olympia (Lepidoptera: Pieridae) In The Great Lakes Area, Warren Herb Wagner Jr.
The Great Lakes Entomologist
[Excerpt]
The dunes along the shores of Lake Huron in the vicinity of Ipperwash Provincial Park, Port Franks, and Pinery Provincial Park, Ontario, are notable for their unusual vegetation and a number of interesting plants and animals. During studies of the peculiar, disjunctive populations of the dwarf hackberry tree (Celtis tenuifolia Nutt.) (Wagner, 1974), I became aware that the local population of marbled white, Euchloe olympia (Edwards), differed in a number of respects from the usual form with which I was familiar. Preliminary observations during the spring of 1975 suggested that the butterflies might even represent a distinct subspecies, so …
First Report Of Fibropapillomatosis And Critical Habitat Use In Green Sea Turtles In Curaçao, M. Tripepi, I. J. R. Van Veghel, A. D. Vreugdenhil, E. Brunelli
First Report Of Fibropapillomatosis And Critical Habitat Use In Green Sea Turtles In Curaçao, M. Tripepi, I. J. R. Van Veghel, A. D. Vreugdenhil, E. Brunelli
College of Life Sciences Faculty Papers
Fibropapillomatosis, a disease affecting green sea turtles (Chelonia mydas), has been documented in many regions of the world, including the Caribbean, but has not been scientifically reported in Curaçao until now. The present study utilized VHF telemetry in Caracas Bay, Curaçao, to track green sea turtles, both healthy and affected by fibropapillomatosis, with the objective of ascertaining their foraging locations. VHF telemetry proved to be a pivotal method for assessing habitat use in areas characterized by limited visibility and high boat traffic, conditions that present significant challenges for divers and snorkellers attempting to access the area. The results …
Lysosomes As Gatekeepers: How Lic1-Mediated Trafficking Regulates Angiogenesis And Vascular Morphogenesis, Dymonn Johnson
Lysosomes As Gatekeepers: How Lic1-Mediated Trafficking Regulates Angiogenesis And Vascular Morphogenesis, Dymonn Johnson
Arts & Sciences Graduate Student Theses and Dissertations
Dynein cytoplasmic 1 light intermediate chain 1 (LIC1, DYNC1LI1) is a core subunit of the dynein motor complex that plays a critical role in cytoplasmic cargo trafficking, including Rab-mediated endosomal recycling and lysosomal degradation. LIC1 interacts with various cargo adaptors, such as RILPL1 and RILPL2, to regulate these processes, and defects in this gene are predicted to impair dynein motor function, Rab binding, and lysosomal trafficking. Here, we identified a dync1li1 zebrafish mutant with a premature stop codon at the exon 12/13 splice acceptor site, that exhibits increased angiogenesis. In vitro, LIC1-deficient human endothelial cells show elevated cell surface levels …
Enumerating Escherichia Coli (E. Coli) Concentrations And Evaluating Optical Brighteners As A Potential Indicator Of Human Wastewater Contamination In La Nana Bayou, Banita Creek, And Their Tributaries, Nacogdoches, Texas, Swastika Acharya, Matthew Mcbroom, Yuhui Weng, Yanli Zhang
Enumerating Escherichia Coli (E. Coli) Concentrations And Evaluating Optical Brighteners As A Potential Indicator Of Human Wastewater Contamination In La Nana Bayou, Banita Creek, And Their Tributaries, Nacogdoches, Texas, Swastika Acharya, Matthew Mcbroom, Yuhui Weng, Yanli Zhang
Electronic Theses and Dissertations
La Nana Bayou was added to the Texas 303(d) list in 2000 after it failed to meet water quality standards set by the Texas Commission on Environmental Quality (TCEQ) for primary contact recreation due to elevated Escherichia coli (E. coli) levels. E. coli is a bacterial species commonly found in the intestines of humans and warm-blooded animals. It serves as an indicator of fecal contamination in freshwater, though its presence alone cannot identify the contamination source. Optical brighteners (OBs), chemicals found in laundry detergents, cleaning agents, toilet papers, and clothing, are unique to human wastewater. This study aimed …
Transcription Fidelity In The Face Of Oxidative Damage, Eastilan Gyiwah Appiah
Transcription Fidelity In The Face Of Oxidative Damage, Eastilan Gyiwah Appiah
Theses and Dissertations
It is very important that the process of transcription is correctly carried out in the cell for the faithful expression of the genetic code. However, should mistakes happen, they can result in transcription errors which interfere with the protein-making machinery of the cell. This will result in accumulation of proteins and dysfunctional proteins that have the potential of causing diseases like Alzheimer’s. Oxidative damage caused by hydrogen peroxide is known to cause transcription errors in organisms like yeast. It is therefore important to investigate the extent to which hydrogen peroxide can damage cells.
Integrated Analysis Of Untargeted Liver, Serum Metabolome And Gut Microbiome Following Selenium Deficiency Diet In Selenoh Knockout And Wildtype Mice, Richard Eugene Baird
Integrated Analysis Of Untargeted Liver, Serum Metabolome And Gut Microbiome Following Selenium Deficiency Diet In Selenoh Knockout And Wildtype Mice, Richard Eugene Baird
Theses and Dissertations
Selenoprotein H (SELENOH) is an oxidoreductase located in the nucleolus, and its biochemical levels decrease with selenium (Se) deficiency, as reported in mammalian research. This current investigation aimed to understand the impact of nutritional Se deficiency on gut microbial composition and metabolomic profiles. The Firmicutes/Bacteroidota (F/B) ratio, linked to various health conditions, was balanced for wildtype (WT) mice (1.06). In contrast, selenoh knockout (KO) mice associated with dietary Se deprivation had a significantly (p < 0.002) lower ratio (0.56), suggesting dysbiosis. It is believed that SELENOH levels decreased due to the Se deficient diet, and the selenoh KO mice exhibited alterations in microbial community composition and metabolite profiles, as supported by correlative analyses. With respect to the bacterial communities, significant differences between diets occurred for three taxa, including Akkemansia mucinphila, Bacteroides caecimuris, and Parabacteroides goldsteinii. Candida tropicalis, Malassezia restricta, and Cyberlindnera jadinii were three abundant fungal yeasts. Only Cyberlindnera jadinii exhibited significantly greater relative abundance between genotypes and was more abundant in the selenoh KO versus WT mice. Correlation analyses between bacterial and fungal taxa revealed both positive and negative relationships, but no specific trends were observed. The liver and serum metabolomic data contained similar amino acids, lipids, proteins, and carbohydrates. Using NMR spectroscopy, 112 liver metabolites were detected, while GC-TOF-MS analysis of the serum revealed the presence of 172, comprising both polar and nonpolar compounds. The metabolite levels varied significantly when analyzed using multivariate models such as PCA and PLS-DA (VIP scores), particularly for glucose, taurine, lactate, and UDP-glucose, which are associated with energy production and cellular health. Overall, the Se deficient diet had the most significant impact on the microbial community structure and the concentration of key metabolites within liver tissues and serum. Correlation analyses conducted across studies revealed significant linear relationships between metabolites and the microbiome. However, these differences were associated with bacterial and fungal taxa containing low relative abundances. Based on these findings, further investigation into the impact of SELENOH homeostasis on microbial and metabolite profiles should be conducted using selenoh KO mice under various nutritional Se dietary levels.
Influence Of Diverse Forage Environments On Honey Bee Colony Health, Angus Catchot Iii
Influence Of Diverse Forage Environments On Honey Bee Colony Health, Angus Catchot Iii
Theses and Dissertations
Honey bees are important pollinators that face a multitude of stressors in their environment such as pathogens, parasite pressure, forage availability and pesticide exposure. This study examined how the makeup of different environments and the stressors related to those environments are affecting honey bee health in Mississippi. A field experiment was conducted during the first year of the study across four distinct landscapes ranging from intensively cultivated cropland to predominantly natural forage habitats to assess the impact of landscape availability on varying honey bee colony health parameters. Locations that had cultivated crops within foraging range produced more honey and brood …
Strategic Use Of Exogenous Feed Enzymes And Sodium Bisulfate To Optimize Broiler Production, Spencer Thomas Karn
Strategic Use Of Exogenous Feed Enzymes And Sodium Bisulfate To Optimize Broiler Production, Spencer Thomas Karn
Theses and Dissertations
Poultry producers must continually evaluate nutritional strategies to enhance broiler performance and sustainability. Chapter II assessed sodium bisulfate (SB) applied to litter, feed, and water across a 42-day grow-out. Applying SB to litter reduced pH, while 0.3% and 0.4% dietary SB improved feed conversion ratio (FCR). Water acidification with SB during the final week did not impair performance, supporting its use as a preharvest intervention. Chapter III evaluated phytase at 500, 1500, and 2000 FTU/kg, with or without protease, in reduced amino acid diets over 56 days. Birds fed a nutrient-adequate diet with 1500 FTU/kg phytase outperformed all reduced-amino acid …
From Gametes To Genes: Genetic Quality Assessment Of Anuran Cryopreserved Spermatozoa And Derived Embryos, Trinity Isabella Kassuhn
From Gametes To Genes: Genetic Quality Assessment Of Anuran Cryopreserved Spermatozoa And Derived Embryos, Trinity Isabella Kassuhn
Theses and Dissertations
Sperm cryopreservation is an important tool within the suite of assisted reproductive technologies (ART) as it enables long-term storage of genetically valuable gametes. However, knowledge of the diagnostic criteria for assessing sperm quality and the genetic consequences of using cryopreserved sperm in amphibians is limited. This gap has raised concerns in captive breeding and reintroduction programs regarding the potential impact that cryopreserved sperm and the derived embryos may have on a population. The thesis aims to investigate how sperm cryopreservation affects the genetic material in both sperm and resulting embryos, with implications for in situ and ex situ amphibian populations. …
Understanding Pollinator-Forb Interactions And Evaluating Trapping Methods For Pollinator Monitoring In Lawns Of The Southeastern United States, Sara Rose Kirby
Understanding Pollinator-Forb Interactions And Evaluating Trapping Methods For Pollinator Monitoring In Lawns Of The Southeastern United States, Sara Rose Kirby
Theses and Dissertations
Urban turfgrass landscapes are widespread across the southeastern United States but offer limited ecological value under conventional management. This thesis evaluates the pollinator support potential of ten forb species commonly found in low-maintenance lawns and assesses the effectiveness of two insect sampling methods, sweep netting and pan trapping. Over two years of field surveys, native forbs such as Symphyotrichum divaricatum and Claytonia virginica supported significantly greater insect abundance and diversity than introduced species. Network and modularity analyses revealed that native plants facilitated more specialized pollinator interactions, including those involving oligolectic bee species. In a complementary study, pan traps effectively sampled …