Digital Commons Network^™

Impact Of Buffered Dry Vinegar And Fermented Onion Juice On The Quality And Shelf-Life Of Fresh Pork Breakfast Sausage, Emily M. Little

Theses and Dissertations

The demand for clean-label meat products has increased interest in natural alternatives to synthetic antioxidants and antimicrobials. This study evaluated the effects of buffered dry vinegar (BDV), fermented onion juice (FOJ), and their combination (CMB) on microbial stability and sensory quality of pork sausage during 9 days of refrigerated storage. Sausages were assigned to control, FOJ (0.5%), BDV (0.5%), or CMB (0.25% each) treatments. BDV and CMB significantly reduced aerobic and psychrotrophic counts by days 7-9, while yeast increased across all treatments over time, reaching ~7 Log CFU/g by day 9. Sensory analysis revealed lower oxidation and off-note intensity in …

Feed-Based Strategies To Improve Poultry Production Efficiency, Anna Kathryn Riggs

Theses and Dissertations

The poultry industry continues to make significant strides in efficiency and innovation, particularly through advancements in nutrition. This thesis explores two key areas within broiler nutrition that are essential to supporting sustainable, cost-effective poultry production. First, the potential of a next-generation exogenous carbohydrase enzyme blend containing beta-mannanase and endo-glucanase is investigated when supplemented into reduced-energy, all-vegetable diets based on corn, soybean meal, and corn distillers dried grains with solubles in male broilers over a 42-day grow-out period, including processing (Chapter II). Second, the thesis examines the use of modeling strategies such as cubic, quadratic, kernel and smoothing spline to predict …

Microbiome–Immune Interaction And Harnessing For Next-Generation Vaccines Against Highly Pathogenic Avian Influenza In Poultry, Yongming Sang, Samuel N. Nahashon, Richard J. Webby

Food and Animal Sciences Faculty Research

Highly pathogenic avian influenza (HPAI) remains a persistent threat to global poultry production and public health. Current vaccine platforms show limited cross-clade efficacy and often fail to induce mucosal immunity. Recent advances in microbiome research reveal critical roles for gut commensals in modulating vaccine-induced immunity, including enhancement of mucosal IgA production, CD8+ T-cell activation, and modulation of systemic immune responses. Engineered commensal bacteria such as Lactococcus lactis, Bacteroides ovatus, Bacillus subtilis, and Staphylococcus epidermidis have emerged as promising live vectors for antigen delivery. Postbiotic and synbiotic strategies further enhance protective efficacy through targeted modulation of the gut microbiota. Additionally, artificial …

Radioactive Contamination And Climate Warming Affect Physiological Performance Of Chornobyl Barn Swallows, Zbyszek Boratyríski, Timothy Mousseau, Anders Pape Møller

Faculty Publications

Global warming and degradation of natural habitats are the two main factors causing ecophysiological stress on individuals and risk for biodiversity. Hyperthermia is a common response to stress in homeothermic animals, in particular to heat, pathogens and environmental contamination. Resilience of biological systems to global warming may be deteriorated in polluted habitats. Here we investigated how body temperature of a wild bird, the barn swallow (Hirundo rustica), responded to global warming while simultaneously exposed to radioactive contamination from the Chernobyl accident. Our results showed that both high air temperatures (t = 15.55, df = 335, p <  0.0001) and elevated environmental radioactive contamination (t = 5.18, df = 8.09, p = 0.0008) increased internal body temperature of individuals. The additive effect suggests that birds might suffer hyperthermia in locally contaminated habitat (1.47% body temperature increase) while simultaneously exposed to globally rising temperatures (1.95% body temperature increase), potentially reducing the fitness of individual and the maintenance of breeding colonies. The cumulative and interactive negative effects of multiple stressors, such as those emerging from increasing habitat degradation and climate change, will likely contribute to biodiversity losses globally.

Immunomodulatory Effects Of Gold Nanoparticles: Impacts On Immune Cells And Mechanisms Of Action, Khadijeh Koushki, Prapannajeet Biswal, Geraldine Vidhya Vijay, Mahvash Sadeghi, Sajad Dehnavi, Ngoc Tuyet Tra, Sai Kumar Samala, Mahdieh Yousefi Taba, Arjun Balaji Vasan, Emily Han, Yuri Mackeyev, Sunil Krishnan

Faculty, Staff and Student Publications

Traditional anti-inflammatory medications-such as corticosteroids, biological agents, and non-steroidal anti-inflammatory drugs-are commonly employed to mitigate inflammation, despite their potential for debilitating side effects. There is a growing need for alternative next-generation therapies for symptomatic, unchecked, and/or detrimental inflammation with more favorable adverse effect profiles. The long history of use of gold salts as anti-inflammatory agents and the more recent exploration of gold nanoparticle (AuNP) formulations for clinical indications suggest that the targeted delivery of nanoparticles to inflammatory sites may be a promising approach worth investigating. Coupled with peptides that specifically target immune cells, AuNPs could potently counteract inflammation. Here, we …

Development Of A Physiologically Based Pharmacokinetic (Pbpk) Model And Evaluation Of Antioxidant Capacity For Polypodium Leucotomos Extract, Christopher L. Basel

Doctoral Dissertations and Projects

The purpose of this study was to develop an equation to calculate the approximate concentration of active components in orally administered Polypodium leucotomos fern extract (PLE) and to develop physiologically based pharmacokinetic (PBPK) models for an active component of PLE or its metabolite to address the need for human pharmacokinetics (PK). Model differences based on ethnic groups were also investigated. This was a quantitative study using secondary data. It has been estimated that skin cancers account for approximately 40% of all human cancers and that approximately 13,000 people will die of skin cancer in 2024. People with darker skin experience …

Nutrition Anthropology In Global Malnutrition In Children Under Five, Katherine Michelle Dennison Owens

Doctoral Dissertations and Projects

Food is essential to growth and development for people and nations. Too little or too much food can lead to undernutrition or overweight, both forms of malnutrition. While there have been improvements in undernutrition, overweight is increasing, causing the double burden of malnutrition globally, which is especially true in low-income and middle-income countries. Understanding nutrition anthropology's economic, environmental, and socio-cultural factors is critical to combat malnutrition's burden in different populations. Shaping policies and investments in malnutrition need to be strategic to have a measurable impact on malnutrition improvements. This study identified nutrition anthropology factors using six economic, environmental, and socio-cultural …

Antifungal Peptides From Casein Milk Of Etawa Crossbreed (Capra Hircus) As Biopreservation Agent For Bread And Molecular Docking Studies, Dian Riana Ningsih, Winarto Haryadi, Rachma Wikandari, Tri Joko Raharjo

Karbala International Journal of Modern Science

Bread without peptide fraction treatment started to grow mold on the fourth day with a total of 24x10⁷ fungi colonies/ml. Meanwhile, the addition of peptide fractions GMC5, GMC6, GMC7, and GMC8 effectively preserved bread for up to 4 days, indicated by no mold growth. The best treatment was the GMC6 peptide fraction on day 8 which grew the least amount of fungus at 7x10⁷ spores/ml. Peptide interaction with the CaATPase receptor Aspergillus sp. was carried out using HADDOCK 2.4. The 3D CaATPase structure prediction resulted in a model with good structural quality. This was supported by residues in …

Scorpions Of The Horn Of Africa (Arachnida: Scorpiones). Part Xlii. Parabuthus Qaraaf Sp. N. From Ethiopia (Buthidae), František Kovařík, Hassan Sh A. Elmi

Euscorpius

No abstract provided.

Novel Nanomaterials For Developing Bone Scaffolds And Tissue Regeneration, Nazim Uddin Emon, Lu Zhang, Shelby Dawn Osborne, Mark Allen Lanoue, Yan Huang, Z. Ryan Tian

Cell and Molecular Biology Faculty Publications and Presentations

Nanotechnologies bring a rapid paradigm shift in hard and soft bone tissue regeneration (BTR) through unprecedented control over the nanoscale structures and chemistry of biocompatible materials to regenerate the intricate architecture and functional adaptability of bone. This review focuses on the transformative analyses and prospects of current and next-generation nanomaterials in designing bioactive bone scaffolds, emphasizing hierarchical architecture, mechanical resilience, and regenerative precision. Mainly, this review elucidated the innovative findings, new capabilities, unmet challenges, and possible future opportunities associated with biocompatible inorganic ceramics (e.g., phosphates, metallic oxides) and the United States Food and Drug Administration (USFDA) approved synthetic polymers, including …

Dopaminergic Neurons In The Zebrafish Subpallium Belong To The Extended Medial Amygdala, Daniel Armbruster, Thomas Mueller, Wolfgang Driever

Department of Biology Faculty Scholarship and Creative Works

The amygdala is a heterogeneous, multinuclear telencephalic structure critical for motivated and emotion-related behaviors in vertebrates. In ray-finned fish (Actinopterygii) like the teleost zebrafish, a telencephalic outward-growing process called eversion makes defining amygdaloid territories particularly challenging. Teleosts are also peculiar in that they develop numerous dopaminergic (DA) neurons in the subpallium, while in tetrapods, such populations are less prominent or appear only transiently. To shed light on the organization of the amygdala in teleosts, we pursued an evolutionary developmental approach focusing on the topological origin of subpallial DA neurons. Specifically, we analyzed the distribution of tyrosine hydroxylase (Th) in conjunction …

Lakota Plant Lesson Connecting Science And Culture For 2nd Graders, Jade M. Jupackal, Larry Browning, Matt Miller, Jiyul Chang, Srinivas Janaswamy, Lydia Yellow Hawk, Jessica Larson, Michael Prate, Madhav P. Nepal

iLEARN Teaching Resources

In this lesson, 2nd graders will learn about how the Lakota people use plants and why these plants are special. They will also discover the important parts of a plant—roots, stem, leaves, flowers, and seeds. Students will engage in activities like coloring, drawing, and exploring real plants found in schoolyard to help them understand each plant part and its function. This culturally responsive lesson blends science with experiential learning and emphasizes plant-people relationships. By the end, students will complete an art-based assessment to demonstrate understanding.

Exploring Soil-Derived Antimicrobials For Inhibiting Dairy-Associated Pathogens And Spoilage Bacteria, Michael Joseph Moran

Theses and Dissertations

Spore-forming bacteria are among the most problematic contaminants in fluid milk. They can survive pasteurization, grow at refrigeration temperatures, and secrete heat-stable enzymes, making them a major cause of spoilage in both pasteurized and reconstituted milk products. Current nonthermal strategies for reducing spore counts, including microfiltration and bactofugation, are often costly and inconsistent. The increasing consumer demand for natural additives in food products has sparked interest in the use of bacterially-derived antimicrobial compounds, particularly those from generally recognized as safe (GRAS) organisms, as an alternative method of dairy preservation. This study explores the antimicrobial potential of ten soil-derived Bacillus and …

The Benefits Of High Phosphate Concentration (10n : 1p) And Diagonal Orientation On The Ex Situ Grow-Out Of Caribbean Coral Juveniles, Ashley Shen

All HCAS Student Capstones, Theses, and Dissertations

As coral reefs keep declining, it is imperative to increase coral production ex situ for restoration, but this requires culture protocols to be optimized. This study examined the impacts of grow-out orientation and phosphate concentration (and nitrate (N): phosphate (P) ratio) on the survival and growth of Montastraea cavernosa and Pseudodiploria clivosa juveniles from 3 to 9 months old. Pristine reefs typically have low nitrate (0.12μmol/L) and low phosphate (0.03μmol/L) concentrations, leading to N:P ratios of 4-16 N: 1P. In recirculating tanks, it is difficult to lower nitrate concentrations below 25μmol/L. Here we hypothesized that increasing the phosphate concentration in …

Three Things We Can Do Now To Reduce The Risk Of Avian Influenza Spillovers, Kenneth B Yeh, William P Bahnfleth, Elaine Bradford, Carol Cardona, Kristen K Coleman, Peter J Hudson, Bernadeta Dadonaite, Rebecca J Hutchins, Anthony Maresso, C Raina Macintyre, Matthew Scotch

Faculty, Staff and Students Publications

No abstract provided.

Buffalo Stories & Science For 4th Graders, Ashley A. Duffy, Larry M. Browning, Matt Miller, Jiyul Chang, Srinivas Janaswamy, Lydia Yellow Hawk, Jessica Larson, Michael Prate, Madhav P. Nepal

iLEARN Teaching Resources

In this culturally responsive science lesson, students explore the ecological and historical importance of buffalo through story and hands-on learning. Using Paul Goble’s Return of the Buffaloes, learners’ journey into Lakota legend to discover how buffalo sustained communities and symbolized connection to land and spirit. Through guided reading, students uncover how buffalo supported Indigenous survival—providing food, shelter, clothing, and tools—while developing an appreciation for traditional knowledge systems and biodiversity. Students then deepen their understanding of food science by making buffalo jerky, connecting past and present preservation methods. As they mix ingredients, shape meat into strips, and observe dehydration techniques, students …

Opposing Roles For Iron Transport Systems In Gallium Tolerance In Extraintestinal Pathogenic Escherichia Coli, Nagama Parveen

Theses and Dissertations

Iron is required for bacterial growth because it serves as a co-factor for enzymes involved in DNA synthesis, cellular respiration, and other processes. Disrupting bacterial iron homeostasis is an effective strategy to prevent the growth of antibiotic-resistant bacteria, and gallium (Ga³⁺) has emerged as a promising candidate due to its ability to displace iron (Fe³⁺) and prevent essential iron-dependent pathways. However, Escherichia coli are naturally less sensitive to gallium than many other bacteria, and the mechanisms that control gallium tolerance are not completely understood. To address this, we performed a genome-wide transposon sequencing (TnSeq) screen to identify genes important for …

Silicon Is The Next Frontier In Plant Synthetic Biology, Aniruddha Acharya, Kaitlin Hopkins, Tatum Simms

Faculty Publications - Biological Sciences

Silicon has a striking similarity to carbon and is found in plant cells. However, there is no specific role that has been assigned to silicon in the life cycle of plants. The amount of silicon in plant cells is species specific and can reach levels comparable to macronutrients. Silicon is used extensively in artificial intelligence, nanotechnology, and the digital revolution, and thus can serve as an informational molecule such as nucleic acids. The diverse potential of silicon to bond with different chemical species is analogous to carbon; thus, it can serve as a structural candidate similar to proteins. The discovery …

Unci Maka’S Gift: An Outdoor Science Adventure For The First Graders, Arlene A. Abao, Larry M. Browning, Matt Miller, Jiyul Chang, Srinivas Janaswamy, Jessica Larson, Lydia Yellow Hawk, Michael Prate, Madhav P. Nepal

iLEARN Teaching Resources

In this super-fun lesson, kids are going outside to explore the schoolyard! They will look for all sorts of amazing plants, also called flora. They will use a special tablet to help us learn their names and then draw pictures of them. They will also dig a little to see the soil and learn why it's so important for plants to grow big and strong. And guess what? They’ll even look for little animals like ants or butterflies, also called fauna, that live near the plants, because everything in nature works together! They'll also hear cool stories about how people …

Sex, Stress And Hormones : The Role Of Modifiable Risk Factors In The Kinetics Of Alzheimer’S Disease, Hannah Marie Edwards

Arts & Sciences Graduate Student Theses and Dissertations

Alzheimer’s Disease (AD) is a neurodegenerative disease hallmarked by the presence of amyloid- plaques and tau tangles in the brain. The process of the disease begins decades prior to the onset of symptoms and is thought to be driven first by a buildup of amyloid- peptide in the extracellular space, triggering a cascade of pathology and symptomology culminating in loss of cerebral volume and cognitive impairment. Women are diagnosed with AD at a rate 2/3 higher than their male counterparts, even when controlling for age. While most driving factors of the disease are thought to be genetic, roughly 40% of …

Tides, Tourists, And Tails: Exploring Human-Wildlife Overlap On The Homer Spit, Alaska, Serena Seiler

Masters Theses & Specialist Projects

Coastal areas present unique challenges for human-wildlife coexistence due to their shared use by people and marine species. The Homer Spit in Homer, AK (59.6481° N, 151.5299° W) is a projection of land into Kachemak Bay on which lodging, restaurants, campgrounds, RV parks, fishing, and a variety of coastal activities exist or occur, especially during the summer months. Two of the most popular nearshore marine mammals that attract visitors are sea otters (Enhydra lutris) and harbor seals (Phoca vitulina). These two species spend time swimming, foraging, and resting around the Spit, potentially resulting in co occurrences with humans. Using a …

Fisheries Research Report No. 350: Recreational Vessel Retrievals In Western Australia - 2009 To 2024, Stephen C. Bradshaw, Claire B. Smallwood, Stuart J. Blight, Troy Matthews, Karina L. Ryan

Fisheries Research Reports

This report provides a time series (2009 – 2024) of the total number of recreational vessel (powerboat) retrievals at a spatial (boat ramp) and temporal (annual, month, hour) resolution for 32 boat ramps distributed from Wyndham to Esperance. This time series has been developed using a workflow that outlines each step, from acquiring raw video data to producing a completed time series of vessel retrievals. As part of this workflow, modelling was undertaken to impute counts of vessel retrieval for sites and hours for which data had not been read, or for which there were significant outages. Features within the …

Fisheries Research Report No. 353: Ecological Risk Assessment For The North Coast Demersal Scalefish Resource, Kimberley A. Smith, Brett Crisafulli, Gabby E. Mitsopoulos, Fabian I. Trinnie, Taylor Grosse, T Thompson, Stephen J. Newman

Fisheries Research Reports

On the 26 March 2025, the Department of Primary Industries and Regional Development (DPIRD) convened an ecological risk assessment (ERA) of the fisheries that access the North Coast Demersal Scalefish Resource (Resource). This Resource comprises a large number of tropical demersal scalefish species that occur in waters of the North Coast Bioregion, including tropical snappers, emperors and cods.

This document contains the results of the ERA along with the background information used to support the risk scoring process. This includes an overview of the Western Australian commercial fisheries that access the Resource, namely the Pilbara Fish Trawl Interim Managed Fishery, …

Assessment Of Social Media Use And Strategies For Combating Misinformation Among Extension Educators In Arkansas, Enoch Teye Kwao Ametepey

Graduate Theses and Dissertations

This study assessed the use of social media by Extension educators in Arkansas and examines strategies for identifying and combating misinformation within agricultural extension services. Guided by the Uses and Gratifications Theory, the Unified Theory of Acceptance and Use of Technology (UTAUT), and the Third-Person Effect Theory for objectives one, two, and three, this research employed quantitative and qualitative approaches, specifically, two quantitative cross-sectional surveys and a qualitative focus group study developed into a three article dissertation. The first article assessed the extent to which Extension educators utilize social media for professional activities, revealing that social media is predominantly used …

Using Published Undergraduate Biomechanics Research On Hydra Mouth Opening To Train Undergraduates, Stephen Hackler, T. Goel, Jonah Pacis , '25, Eva-Maria S. Collins

Biology Faculty Works

Biophysics research is exciting because physical approaches to biology can provide novel insights, and it is challenging because it requires knowledge and skills from multiple disciplines. We have developed an undergraduate biophysics laboratory module that teaches fundamental skills such as time-lapse microscopy, image analysis, programming, critical reading of scientific literature, and basics of scientific writing and peer review. The module is accessible to students who are familiar with introductory statistics, cell biology, and differential calculus. We used published research on the biomechanics of Hydra mouth opening as a framework because it describes a stunning biological phenomenon: Hydra, a freshwater …

Influence Of Exercise Opportunities On Bone Health In Laying Hens, Jaden Costner, Alexis Clark, Ahmed Ali

Publications

Laying hens are often housed in environments that restrict movement, which may contribute to bone fragility and decreased welfare. These confined environments, such as conventional cage systems, limit exercise and result in poor skeletal development. This study investigates the impact of exercise opportunities on bone health in laying hens by comparing the tibiotarsi and humeri bone characteristics in enriched and unenriched housing environments. A total of 200 hens were housed in pens (20 birds/pen) from 0 to 40 weeks of age. Five pens, which served as the experimental group, were equipped with multi-tier perches and ledges, providing exercise opportunities. In …

Fisheries Research Report No. 361: 2024 Assessment Of The Status Of The Elasmobranch Resource Of Western Australia, Matias Braccini, Alex Hesp

Fisheries Research Reports

This document provides the 2024 risk-based weight of evidence stock assessment for the elasmobranch (sharks and rays) resource of Western Australia (WA). Gummy (Mustelus antarcticus), dusky (Carcharhinus obscurus), whiskery (Furgaleus macki), and sandbar (C. plumbeus) sharks are the most commonly captured species (~80% of the elasmobranch catch) and have been selected as indicator species for the status of the temperate elasmobranch ‘suite’. However, over 100 elasmobranch species have been caught in commercial and/or recreational fisheries in WA and, with the increasing number of elasmobranch species being listed in national and international protection lists, species-specific scientific advice on stock status at …

Mosquitoes: The 5 C’S Of Developing Disease- Commensals Changing To Consequential Pathogens, Alan L. Gillen, Caleb Mast, Robert Braden Somerville

Faculty Publications and Presentations

Hotter, longer summers and changing weather patterns in the United States are bringing more than just sweat and discomfort. In May 2025, a summer heat wave of 107⁰F hit Austin, Texas, and in humid Houston and San Antonio, Texas, temperatures felt over 100⁰ for several days. Normally, these temperatures don’t come until July and August. With temperatures reaching these marks early and heavy rains coming, mosquito activity and ranges expand. Many state and federal health officials report a rise in mosquito-borne illnesses across the US and globe in the last five years.

The Social Dimensions Of Aquaculture: Food Sovereignty And Indigenous Rights In Ketchikan, Alaska, Morgan J. Davis

Capstone Collection

This research project asks how the growing commercial aquaculture industry in Ketchikan, Alaska, functions to alleviate or exacerbate local food insecurity; reinforce or undermine food sovereignty among Indigenous communities, and enhance or restrict community participation in the control of marine resources. Global initiatives have responded to food insecurity through agricultural inputs and technology to create higher yields; however, conventional agricultural production has contributed to and is increasingly vulnerable to climate change. To address higher demands for food, government agencies and development experts have looked towards Blue Growth and aquaculture as a solution for food insecurity and environmental concerns. While aquaculture …

Metabolic Regulation Of Cardiac Fibroblast Phenotype And Function., Collin Kleis Wells

Electronic Theses and Dissertations

Cardiac fibroblasts are central effectors of cardiac repair after myocardial infarction (MI). In response to signaling cues, they differentiate to a range of phenotypes with robust capacities to synthesize and secrete extracellular matrix (ECM) and signaling molecules. Although activated fibroblast phenotypes are associated with pronounced changes in metabolism, it remains unclear how the metabolic network upholds the effector functions of fibroblasts in the post-infarcted heart. Here, we identified that critical enzymes in the phosphoenolpyruvate (PEP) cycle, i.e. pyruvate kinase muscle-2 (PKM2) and phosphoenolpyruvate carboxykinase-2 (PCK2), are elevated in the heart after MI and examined their role in regulating post-MI remodeling …