Digital Commons Network^™

Development Of Broadly Protective Vaccines Against New World Arenaviruses, Brian B. Gowen

Funded Research Records

No abstract provided.

Role Of Recreational Fisher Information In Fisheries Management, Rebecca M. Krogman, Stephen M. Taylor, Aaron J. Adams, Ross E. Boucek, Steven M. Lombardo, Chelsey A. Crandall, Susan F. Steffen, Alejandro Acosta, Eduardo Bone Moron, Sepp Haukebo, Kieran Hyder, Zachary M. Radford, Warren M. Potts

Books & book chapters

Fisheries managers work with the three components of fisheries—biota, environment, and people - to achieve recreational, stewardship, and socioeconomic goals. Although manipulation of biota and the aquatic environment remains popular (e.g. harvest regulation, protecting or enhancing habitats, and guarding water quality), the human dimension of fisheries must also be considered throughout the fisheries management process. Managers can and should engage fishers in a myriad of ways, from identification of problems to guidance on alternatives, and from measurement of change to evaluation of success. In this chapter, we review common fisheries management goals and objectives; detail information needs stemming from those …

Protocols For Antioxidant Testing: A Mini Review Of Common Assays And Approaches, Abdulsalam Halboup, Sameer A. Alkubati

AUIQ Complementary Biological System

Antioxidants play a pivotal role in mitigating damage caused by oxidative stress, which is implicated in aging, inflammation, neurodegeneration, and various chronic diseases. With the growing interest in natural and synthetic antioxidants across nutraceutical, pharmaceutical, and food industries, the need for reliable, reproducible, and standardized testing methods has become increasingly urgent. This mini-review provides a structured overview of the most widely used antioxidant testing protocols, categorized into in vitro chemical-based assays (e.g., DPPH, ABTS, FRAP, metal chelation), cell-based assays (e.g., DCFH-DA for intracellular ROS detection), and in vivo biomarker-based models. Each method’s principle, experimental protocol, advantages, and limitations are critically …

Prevalence Of Giardia Infection: A Cross-Sectional Study In Basra Province, Iraq, Sarah Kamal Naser

AUIQ Complementary Biological System

Giardiasis is a common parasite infection that is seen more frequently than any other parasitic infection worldwide. This infection is especially prevalent in tropical areas and in developing nations that have a poor standard of living and poor health conditions. The Giardia parasite is able to infect people of any gender, as it enters the body by the mouth, passes down the oesophagus, and finally reaches the stomach, where it is not negatively impacted by gastric acids. The goal of this study is to determine the extent to which patients who were treated at Al-Zubair General Hospital in Basra, Iraq, …

Neurobehavioral Mechanisms Of Psychotic-Like Experiences And Cannabis Use, Ethan Campbell

Psychology ETDs

As cannabis use has increased steadily over the past decade, there is critical need to evaluate early cannabis use as a potential risk factor for the development of psychosis. Neurobehavioral mechanisms underlying novelty salience could clarify how cannabis exposure may influence subclinical psychotic-like experiences (PLEs). The present study leveraged dynamic functional connectivity and task-fMRI to assess the role of novelty processing in cannabis use and PLEs. A group of brain regions dynamically tied to the salience network while viewing short films were found to increase their activation as a function of stimulus novelty during a novelty bandit task. PLEs were …

Stress During Adolescence: A Characterization Of Anxiety-Like Behavior, And The Mrna Expression Of Neuroimmune Factors, Clock And Stress Genes In Corticolimbic Areas., Annette Fernandez Oropeza, Annette K. Fernandez Oropeza

Biomedical Sciences ETDs

Adolescence represents a critical window of frontal cortex (FC) maturation and corticolimbic circuit refinement that supports stress and emotion regulation. Disruption of these processes by early-life insults such as prenatal alcohol exposure (PAE) or adolescent stress increases vulnerability to anxiety and mood disorders, in part through circadian–immune dysregulation. The circadian transcription factor Bmal1 and the proinflammatory cytokine tumor necrosis factor-alpha (TNFα) have emerged as key regulators of neuroimmune signaling and synaptic communication, yet their interactions across developmental risk contexts remain poorly understood. This dissertation examined the effects of mild adolescent isolation stress, with and without PAE, on anxiety-like behaviors and …

Listen To The Birds: Sound Ecology At The Piano, Anna Showalter

Dissertations, 2020-current

This document explores the intersection between birdsong and piano music using four cases studies from the piano repertoire in the Western classical tradition. Using Jeff Titon’s framework of “sound ecology” as a lens, I ask whether the following pieces of music could in some way function as an “acoustic tissue among human and bird communities that becomes the basis for a healthy economy and a just community.” After exploring the history of birdsong in the piano literature as a microcosm of shifting attitudes toward birdsong in Western art music this study focuses on four pieces as case studies for analysis—Amy …

A Halloween Decoration’S Scary Implications For Real Bats, Coralie Carlson

Capstones

This investigative article examines the online illegal trade of taxidermied bats and how the sales are threatening one particularly showy species: the painted woolly bat.

My OSINT research shows where the bats are still sold – from Amazon to independent websites offering bulk orders – and tracks the sellers and buyers, while weaving in the scientific and cultural history this little-known colorful bat and the conservationists’ race against time to protect them.

The very existence of the painted woolly bat is threatened by Americans who want to display the dead bats in their homes as spooky decorations. Researchers (including a …

Metamaterial-Based Microstrip Antenna For Safe Detection Of Tiny Breast Tumors, Shrouk E. El-Sawy, Mohamed G. Abdelfattah, Nihal F.F. Areed, Ahmed Shaban Samra

Mansoura Engineering Journal

This study describes the construction of a compact microstrip patch antenna (MPA) with a triangular-shaped metamaterial layer for the safe detection of tiny breast tumors at 2.4 GHz. The metamaterial considerably improves the antenna's gain and directivity when compared to typical designs that do not include it, resulting in improved detection performance. The radiating patch of proposed antenna measures 29.3 × 38 × 1.6 mm and has been tested through simulations and experimental tests, exhibiting successful tumor identification in a realistic breast phantom. The results of both calculations and testing demonstrate a sensitivity of 6.643 dB/mm, allowing for precise detection …

Variation In Nectar Rewards Under Water Limitation Across Flowering Species, Madeline Isabelle Gregor

Undergraduate Honors Theses

Drought is becoming an increasingly dominant force shaping plant–pollinator interactions, yet we still know little about how nectar rewards respond to water limitation across species commonly used to support pollinators. We examined nectar volume and sucrose content in six flowering species relevant to California’s Mediterranean climate and quantified how drought alters these traits for four of those species. Under well-watered conditions, species differed in their nectar production, spanning more than two orders of magnitude. Perennial species such as Epilobium canum and Scrophularia californica produced the highest nectar volumes and sucrose amounts, suggesting that they contribute substantial carbohydrate resources when moisture …

Effects Of Beach Renourishment On Sea Turtle Nesting Success, Caydence Pennington

Honors Theses

Beach geomorphology has a profound influence on nesting animals, particularly sea turtles, whose reproductive success depends on suitable sand conditions and stable shorelines. In many densely populated coastal regions, beach renourishment projects are implemented to combat erosion and protect infrastructure, yet these alterations can also affect sea turtle nesting ecology. This study evaluated the effects of beach renourishment on loggerhead sea turtle (Caretta caretta) nesting activity on Longboat Key, Florida, from 2021-2024. Nesting activity, hatch success, and depredation patterns were compared between nourished and reference beach zones to determine whether nourishment had an impact on these events. Nesting …

Determinants Of Farmers’ Choices Of Adaptation Strategies In Rural Semi-Arid Agricultural Communities Of Tanzania: A Case Study Of Mwanga And Same Districts In Kilimanjaro Region, Bahati Ally Magesa

Journal of Humanities and Social Sciences

Climate change is a global phenomenon that poses a significant threat to agricultural farming and the livelihoods of farmers, particularly in semi-arid regions of Sub-Saharan Africa. Since agriculture is the primary source of employment in the region, enhancing the sector’s adaptive capacity has become increasingly important. This paper examines the key determinants influencing farmers’ choices of adaptation strategies to climate change impacts in the Mwanga and Same Districts of Tanzania. A total of 200 farmers participated in a household survey conducted using a simple random sampling technique. A binary logistic regression model was employed to analyse the association between selected …

Understanding Outer Membrane Vesicle Biogenesis In Bacteroides Thetaiotaomicron, Evan J. Pardue

Arts & Sciences Graduate Student Theses and Dissertations

Outer membrane vesicles (OMVs) are small proteoliposomes generated by active protrusion of the outer membrane (OM) in Gram-negative bacteria during growth. Over 60 years of experimentation has ascribed numerous functions to OMVs produced by different bacteria. However, the mechanism(s) by which OMVs are produced is poorly understood. Due to this, the field remains controversial because many regard OMVs as byproducts of cell lysis and question their physiological relevance. Previous studies in Pseudomonas aeruginosa and related species confirmed that these microbes undergo programmed cell death that results in the stochastic release of vesicles and cellular contents. On the contrary, Bacteroides spp. …

Heat Increment Of Feeding In The Common Bottlenose Dolphin (Tursiops Truncatus) Contributes Moderately To Field Metabolic Rate Estimates, Ioulia Koliopoulou, Stacy L. De Ruiter, Jordi Altimiras, Josefin Larsson, Julietta Arenarez, David Rosen, Andreas Fahlman

University Faculty Publications and Creative Works

Digestion elevates metabolism through the heat increment of feeding (HIF) – the energy expended on mechanical and biochemical processes after eating. Quantifying this cost is essential for bioenergetic models that predict energy flow and prey requirements in populations. Using breath-by-breath respirometry, we measured oxygen consumption (V̇ O2 ) in eight common bottlenose dolphins (Tursiops truncatus) before and after feeding standardized meals (1659–2658 kcal of capelin and herring). Metabolic rate rose by ∼37% above resting levels, peaking 60 min after feeding before returning to baseline within 2 h. When scaled across the day, digestion increased daily metabolic needs by ∼8.2% of …

Sex Disparities In Prescription Opioids Associated Cardiovascular Adverse Events, Li Ma

Theses and Dissertations

The opioid crisis in the United States has evolved from a medical response to pain into a nationwide public health emergency. The prescription opioids (POs) – associated cardiovascular adverse events (CVAEs) remain insufficiently studied, especially in relation to sex disparities. This dissertation investigates sex disparities in PO-associated cardiovascular adverse events (CVAEs) through an analytic framework integrating artificial-intelligence-assisted literature mining and real-world pharmacovigilance analytics. In the analytic framework, first, AI powered topic modeling was employed to uncover POs-associated CVAEs risks with a focus on sex differences. A total of 1,837 PubMed abstracts were analyzed using two methods, Latent Dirichlet Allocation (LDA) …

Agpat2 Acts At The Crossroads Of Lipid Biosynthesis And Drp1-Mediated Er Morphogenesis, Yoshihiro Adachi, Mauricio Torres, Allen H Hunter, Woo Jung Cho, Meixia Pan, Xianlin Han, Guangwei Du, Ling Qi, Miho Iijima, Hiromi Sesaki

Faculty, Staff and Student Publications

The morphology of the endoplasmic reticulum (ER), characterized by central sheets and peripheral tubules, is controlled by membrane-shaping proteins. However, the role of lipids in ER morphogenesis remains elusive, despite the ER being the major site for lipid synthesis. Here, by examining the role of eighteen phosphatidic acid (PA)-generating enzymes in ER morphology, we identify lysophosphatidic acid acyltransferase 2 (AGPAT2) as a critical factor in mouse and human cells. AGPAT2 produces PA in the glycerophospholipid/triacylglycerol biosynthesis pathway, and its mutations cause congenital generalized lipodystrophy. We find that AGPAT2-generated PA drives ER tubulation through gene knockout, 3D structural analysis by FIB-SEM, …

How The Intake Of Pulses May Impact Metabolic Disorders And Dementia Risk: A Narrative Review, Lisa Michelle Belzer Salinas, Maricarmen Marroquin, Mariana Mendez, Isabel Omaña-Guzmán, Juan Carlos López-Alvarenga

Health & Biomedical Sciences Faculty Publications

We present a narrative review focusing on pulses’ geographical origin and distribution, their impact on human evolution and history, and their influence on human health. Pulses, including dry peas, beans, and lentils, are renowned for their richness in chemical antioxidants. Despite containing antinutrients, processing techniques preserve their health advantages. Epidemiological research has consistently demonstrated that the consumption of pulses is associated with favorable effects on metabolism. This evidence is further supported by molecular and clinical research, which has elucidated potential nutrigenomic mechanisms and effects on gut microbiota composition underlying their health benefits. However, the literature lacks randomized controlled clinical trials …

Study Of Insect Frass-Associated Microbiome On Plant Growth And Their Potential Use As Biofertilizer, Jasmine Sahota

Theses and Dissertations

An increasing global food demand and the urgent need for sustainable agricultural practices have driven interest in insects as alternative feed and food sources. Insect frass, a by-product of insect rearing, represents a rich organic carbon source with diverse microbes that can enhance soil health and promote beneficial plant-microbe interactions. This study characterized the bacterial (16S-V4) and fungal (ITS2) microbiomes of frass from Black Soldier Fly (BSF) and Cricket species and evaluated their effects on maize (Zea mays) growth. Four frass variants were applied at rates of 56-280 kg ha⁻¹ in a pot experiment with untreated soil and mineral fertilizer …

The Biology Of Green Antelopehorn Milkweed (Asclepias Viridis Walter) And Hemp Dogbane (Apocynum Cannabinum L.) And Response To Triclopyr Formulations And Other Auxin Herbicides, Kayla L. Broster

Theses and Dissertations

Studies were conducted in Oktibbeha and Clay Counties in Mississippi to determine the response of green antelopehorn milkweed and hemp dogbane to triclopyr and other auxin herbicides in non-cropland areas. These studies were conducted at four locations with well established populations for each species throughout 2020 to 2023, with applications repeated at each location the following year if populations persisted. Treatments included a high and low rate of each herbicide studied, with all four triclopyr formulations included for both species. Injury response data were collected two and four weeks after treatment and population density data were evaluated using individual stem …

Trace Mineral Supplementation Strategies For Beef Cattle During Preconditioning And Feedlot Receiving, Madeline G. Mcknight

Theses and Dissertations

Beef cattle routinely undergo a variety of stressors within the preconditioning and feedlot receiving phases which may impair animal performance and health. Trace mineral supplementation, either through dietary or injectable sources, is proposed to mitigate these effects through metabolic and immune system support. Two systematic reviews and meta-analyses were conducted to evaluate the efficacy of two different oral supplementation methods, organic (OTM) and inorganic (INR), and injectable trace mineral (ITM) solutions on average daily gain (ADG) and morbidity. Across 20 studies, OTM supplementation increased ADG (P = 0.01) when compared to INR, but did not affect morbidity (P = 0.92). …

Spatiotemporal Ecology Of Mycobacterium Ulcerans And Mycolactone Functions In French Guiana And Southeastern United States Aquatic Environments, Magdalene Dogbe

Theses and Dissertations

Mycobacterium ulcerans, the causative agent of Buruli ulcer, produces mycolactone, a polyketide toxin traditionally studied for its role in human pathogenesis. This dissertation presents a comprehensive spatiotemporal analysis of M. ulcerans and mycolactone producing mycobacteria (MPM) ecology in the Americas and also reveals mycolactone's role in the environment as a microbial community regulator. Through comparative studies in French Guiana (endemic) and the southeastern United States (non-endemic), combined with experimental microcosm investigations, this research contributes to environmental pathogen ecology understanding. Environmental surveillance across 2,226 samples from French Guiana revealed complete MPM restriction to freshwater environments, with zero detections from 1,226 coastal …

Influence Of Urbanization On Diel Activity Patterns Across Seasons For Four Mesocarnivore Species Along An Urban-To-Rural Gradient Through Boston, Massachusetts, Myra M. Goes

Honors Program Theses and Projects

Urbanization is a growing threat to wildlife and poses unique challenges that animals must overcome to thrive in developed places. Temporal partitioning is one method that wildlife may employ to persist among the increased predation, competition, and human presence pressures of urban spaces. To evaluate diel patterns of mesocarnivores along an urban-to-rural (URG) gradient, data were utilized from camera traps established at 27 sites along a URG in southeastern Massachusetts as part of the Urban Wildlife Information Network (UWIN). Camera trap photos from summer and winter between 2023 to 2025 were used and filtered for coyote (Canis latrans), northern raccoon …

Reducing Foodborne Bacteria Public Health Burden By Novel Compounds And Pressure-Based Technologies, Junice Alannah Sibley

Tennessee State University Alumni Theses and Dissertations

Food safety strategies are being developed in the hopes of combating foodborne pathogens and their risks. Previous techniques and technologies are struggling to meet the needs of the public, leading to the demand for new, innovative methods. Mild high-pressure processing (HPP) of 350 MPa was used in a synergetic combination with mild heat of 60°C and the antimicrobial carvacrol for the decontamination of non-typhoidal Salmonella serovars that resulted in a >5 log CFU/mL reduction. Additionally, a secondary study was conducted comparing a single Salmonella Tennessee strain with a 4-strain Salmonella cocktail to examine sensitivity to pressure-based treatments resulting in a …

Agronomic Management Practices For Cbd And Fiber Hemp In Southeastern United States, Anand Kumar

Tennessee State University Alumni Theses and Dissertations

The farm bill of 2014 and 2018 legalized hemp (Cannabis sativa L.) production for cannabidiol (CBD), grain, and fiber uses in the U.S. Agronomic management practices for floral hemp for CBD production were not well studied. To optimize the agronomic management practices for high CBD content and THC concentration below the compliance level of 0.3% on a dry weight basis requires specific management and adaptability. A two-year field study was conducted at the Tennessee State University Agricultural Research and Education Center (TSU AREC) in Nashville, Tennessee, to evaluate two production systems, the Non-Chemical System (NCS) and the Chemical-Based System (CBS), …

Exploring The Autofermented Cyanobacterial Crude Extract As Antimicrobial Animal Feed Additives., Mohana Krishnan Neelakrishnan

Tennessee State University Alumni Theses and Dissertations

Overexploitation of antibiotics in the animal industry has driven the need for reduced usage, but this reduction has intensified the challenge posed by Salmonella enterica and Campylobacter jejuni to livestock systems and public health. This situation underlines the need for sustainable antimicrobial alternatives. Although cyanobacteria are promising in this respect, since they may produce a variety of bioactive metabolites, the amounts of these metabolites in their natural state are insufficient to elicit potent antibacterial action. In this study, autofermentation was investigated as a means of enhancing antibacterial efficacy in Fischerella ambigua and Nostoc muscorum by promoting the synthesis of organic …

Structural Basis Of Panx1 Permeation And Positive Modulation By Mefloquine, Yangyang Li, Zheng Ruan, Junuk Lee, Ian Orozco, Edward Zhou, Juan Du, Wei Lü

Department of Biochemistry and Molecular Biology Faculty Papers

Purinergic signaling relies on ATP release through exocytosis and large-pore channels. Large-pore channels permeate both small anions like chloride and large signaling molecules like ATP, but how this broad cargo selectivity is structurally controlled remains elusive. Here we investigate PANX1, a prototypical large-pore channel, and uncover structural plasticity at the extracellular entrance formed by seven tryptophan (W74) residues. The W74 sidechains are flexible, sampling conformations that range from a constricted state permissive only to chloride to a dilated state compatible with ATP. These states are coupled to variable cation-π interactions between W74 and arginine 75 (R75), suggesting a mechanism for …

Predicting, Culturing, And Characterizing Beneficial And Detrimental Strains Associated With Plant Phenotypes., Anastasiia Onyshchenko

Arts & Sciences Graduate Student Theses and Dissertations

Modern agriculture’s reliance on chemical fertilizers has greatly increased crop yields but has also contributed to soil degradation, environmental pollution, and unsustainable resource use. Plant growth–promoting rhizobacteria offer a sustainable alternative, enhancing plant growth through mechanisms such as nitrogen fixation, phosphate solubilization, siderophore production, and phytohormone modulation, while also protecting plants against pathogens and abiotic stresses. However, soil microbial communities are highly diverse and structurally complex, making the isolation of individual beneficial strains challenging. Traditional approaches rely on selective media and labor-intensive phenotypic screening, which often capture only a small fraction of soil diversity. To overcome these limitations, we combined …

Microplastic Exposure Alters Tubulin Leading To Alterations In Insulin Secretion By Pancreatic Beta Cells, Anakaren Garcia

Honors Program Theses and Research Projects

Abstract

Diabetes mellitus is a metabolic disorder characterized by elevated blood glucose levels due to impaired insulin secretion or diminished cellular response to insulin (resistance). Pancreatic β-cells depend on a well-organized cytoskeleton, particularly microtubules composed of α- and β-tubulin heterodimers, to transport insulin granules to the plasma membrane during glucose-stimulated insulin secretion (GSIS). Disruption of microtubule organization can impair granule trafficking and reduce insulin release. However, emerging environmental toxicants such as microplastics that interfere with β-cell cytoskeletal function remains largely unexplored.

Microplastics (MPs) are small plastic particles (< 5 mm) that have become widespread environmental contaminants and can enter biological systems. Previous studies have shown that MPs can induce cellular stress and protein misfolding, but their effects on microtubules in pancreatic β-cells are not well characterized. This study investigates whether exposure to fluorescent polystyrene microplastics alters microtubule organization and insulin secretion in βTC-6 cells.

βTC-6 cells were treated with 10 µg/mL of fluorescent polystyrene microplastics for …

White Nose Syndrome In Bats And Infant Mortality In Georgia, Rhys Medcalfe, Sean Medcalfe, Simon Medcalfe

Journal of the Georgia Public Health Association

Background: White nose syndrome (WNS) is a disease in bats caused by the fungus Pseudogymnoascus destructans that can result in bat mortality rates of over 90%. WNS was discovered in Georgia in 2012 in 3 counties but has continued to spread to 14 counties as of 2023, with P. destructans detected in a further seven. Biodiversity loss has been linked to human health outcomes including infant mortality. Understanding the link between WNS, biodiversity loss, and infant mortality in Georgia is important because Georgia ranks 42^nd out of the 50 states for infant mortality rate.

Methods: Using panel data from …

Seed Amplification Of Msa Alpha-Synuclein Aggregates Preserves The Biological And Structural Properties Of Brain-Derived Aggregates, Fei Wang, Victor Banerjee, Carla Barria, Santiago Ramirez, Tyler Allison, Damian Gorski, Haley Evans, Quynh Nguyen, Danielle Harrison, Rabab Al-Lahham, Nicole De Gregorio Carbonell, Michelle Pinho, Sanne Kaalund, Jonas Folke, Susana Aznar, Luis Concha-Marambio, Mohd Ishtikhar, Venkata Kps Mallampalli, Sandra Pritzkow, Mohammad Shahnawaz, Matthew L Baker, Irina Serysheva, Claudio Soto

Faculty, Staff and Student Publications

Parkinson's disease (PD), Dementia with Lewy bodies (DLB), and multiple system atrophy (MSA), are characterized by the misfolding and aggregation of alpha-synuclein (αSyn). Compelling evidence showed that αSyn aggregates exist as distinct conformational strains in different synucleinopathies. Recently, we reported that the αSyn Seed Amplification Assay (αSyn-SAA) can amplify and distinguish αSyn strains from PD and MSA. In this study, we investigate whether MSA-seeded, SAA-amplified αSyn fibrils retain the biological and structural properties of the αSyn seeds present in MSA brains. We study the biological activities of both brain-derived and SAA-amplified αSyn aggregates using an αSyn "biosensor" cell model and …