Digital Commons Network^™

Dihydroethidium-Derived Fluorescence In Electrically Stressed Cells Indicates Intracellular Microenvironment Modifications Independent Of Ros, Esin B. Sözer, Iurii Semenov, P. Thomas Vernier

Bioelectrics Publications

Intracellular reactive oxygen species (ROS) generation is widely suggested as a trigger for biological consequences of electric field exposures, such as those in electroporation applications. ROS are linked with membrane barrier function degradation, genetic damage, and complex events like immunological cell death. Dihydroethidium (DHE) is commonly used to monitor ROS in cells. DHE is linked to intracellular ROS by a primary oxidation product, Ethidium (Eth⁺), that shows increased fluorescence upon binding to polynucleotides. We observed changes in DHE-derived fluorescence in Chinese hamster ovary (CHO) cells post 300-ns electric pulse exposures, comparing them to tert-butyl-hydroperoxide (t-BHP) induced oxidative stress. …

Mouralherwaqh Coastal Wetland Road Crossing Da'luk, Natalie Mendez

Cal Poly Humboldt theses and projects

The integration of Indigenous and Western science plays an essential role in Tribally led collaborations for land management. This process of woven sciences is rooted in reciprocal relations and partnerships guided by Tribal Nations. Our cohort was invited by Wiyot Tribal Representatives to investigate a culvert located within the wetlands of Mouralherwaqh— a parcel of land reacquired by the Wiyot Tribe in 2022. This document seeks to share our experience and analysis as part of the Wiyot Tribe’s broader journey in navigating ecocultural restoration projects within Mouralherwaqh. The four community interests we investigated for the wetland crossing included a resized …

Synergistic Effects Of Nanosecond Pulsed Plasma And Electric Field On Inactivation Of Pancreatic Cancer Cells In Vitro, Edwin A. Oshin, Zobia Minhas, Ruben M. L. Colunga Biancatelli, John D. Catravas, Richard Heller, Siqi Guo, Chunqi Jiang

Bioelectrics Publications

Nanosecond pulsed atmospheric pressure plasma jets (ns-APPJs) produce reactive plasma species, including charged particles and reactive oxygen and nitrogen species (RONS), which can induce oxidative stress in biological cells. Nanosecond pulsed electric field (nsPEF) has also been found to cause permeabilization of cell membranes and induce apoptosis or cell death. Combining the treatment of ns-APPJ and nsPEF may enhance the effectiveness of cancer cell inactivation with only moderate doses of both treatments. Employing ns-APPJ powered by 9 kV, 200 ns pulses at 2 kHz and 60-nsPEF of 50 kV/cm at 1 Hz, the synergistic effects on pancreatic cancer cells (Pan02) …

A Binary Integer Linear Programming Model For Optimizing Underground Stope Layout, Theophilus Mensah

Masters Theses

"Underground mine planning engineers face significant challenges when determining what geometry provides the most profitable and safe stope for extraction. Several techniques and optimization algorithms have been developed in recent years, but most fail to find optimal solutions because they are heuristic or LP-based without efficient geometric constraints. This thesis work proposes a two-dimensional binary linear programming (BILP) model for determining the optimal combination of blocks in a stope that maximizes the economic value of the layout of stopes for a sublevel deposit. The work draws from Queyranne and Wolsey’s (2017 & 2018) formulations of tight constraints for bounded up/down …

Parametric Study Of The Effect Of Increased Magnetic Field Exposure On Microalgae Chlorella Vulgaris Growth And Bioactive Compound Production, Sharanabasaweshwara Asundi, Sanurag Rout, Simone Stephen, Sanghamitra Khandual, Sandipan Dutta, Sandeep Kumar

Mechanical & Aerospace Engineering Faculty Publications

This parametric study aimed to analyze the effects of increased magnetic field exposure (MFE) on the growth and production of the bioactive compounds of Chlorella (C.) vulgaris. With the intent of studying the effect of an increased MFE, the magnetic field typically experienced by life on Earth was amplified by an order of magnitude. In the increased-MFE environment, six treatments of C. vulgaris with two repetitions for each treatment were exposed to a magnetic field of 5 Gauss (500 µT) about each axis, which was generated in a state-of-the-art Helmholtz cage. The treatments and the control were characterized by the …

Kinodynamic Motion Planning For A System With Squid Dynamics, Logan E. Beaver, Cong Wei, Wei-Kuo Yen

Mechanical & Aerospace Engineering Faculty Publications

This paper introduces a path planning algorithm for a system with squid dynamics in a cluttered environment. We capture the complex interactions of fin, arms, and body patterning by analyzing experimental data collected from observing squid motion. We extract nine motion primitives to build the control sequence for a time-optimal trajectory. This task is formulated as a mixed-integer program, and we generate the minimum-time trajectory using a sample-based approach. Numerical simulations illustrate the efficacy of this strategy and motivate ongoing and future efforts to exploration of squid motion features, improvement of the modeling, and experimental demonstrations of the motion planning …

Ring Opening Functionalization Of Thiolactone Homopolymers For Ros Scavenging, Aaron Priester, Forrest Kievit, Anthony J. Convertine

Materials Science and Engineering Faculty Research & Creative Works

In This Research, We Explored the Synthesis and Application of Thiolactone Acrylamide (TLA) Polymers for Neutralizing Reactive Oxygen Species (ROS), Produced as a Result of Traumatic Brain Injury (TBI). We Synthesized Well-Defined TLA Polymers using RAFT (Reversible Addition-Fragmentation Chain Transfer) Polymerization Across a Range of Monomer-To-Chain Transfer Agent ([M]0/[CTA]0) Ratios to Achieve a Range of Molar Masses. Polymerizations Were Carried Out using a Trithiocarbonate CTA (Chain Transfer Agent) with AIBN (Azobisisobutyronitrile) as the Initiator in N'N-Dimethylacetamide (DMAc) at 60 °C. Kinetic Studies Indicated a Linear Increase in Molar Mass with Conversion, Pseudo-First-Order Kinetics, and Molar Mass Values that Are Consistent …

Ecological Toxicology Impact From Soap Manufacture: Applications Of Carbon Footprint For Organization And Carbon Footprint Of Product, Tanaporn Chanaphirak

Chulalongkorn University Theses and Dissertations (Chula ETD)

The assessment of greenhouse gases to identify ways to reduce global warming was of enormous importance worldwide. This study assessed the carbon footprint of an organization and the carbon footprint of products within the herbal soap manufacturing industry, following the guidelines of the Thailand Greenhouse Gas Management Organization (TGO). It also evaluated the environmental toxicity of soap production. The study found that the organization’s carbon footprint amounted to 551 tCO2 equivalent, with Scope 3 emissions being the highest at 359 tCO2 equivalent, followed by Category 1 products and services, which accounted for 208 tCO2 equivalent. Scope 1 emissions were 176 …

Optimization Of Energy From Building Integrated Photovoltaic Of High-Rise Buildings In Thailand, Min Khant

Chulalongkorn University Theses and Dissertations (Chula ETD)

This project is to find the method to optimize the energy from Building Integrated Photovoltaic System in high rise building. Firstly, the solar irradiance potential of vertical surfaces of high-rise building is analyzed in Bangkok, Thailand. With these irradiance values, the optimal size and placement of BIPV module on east, west and south façades of the building is analyzed in order to minimize the building energy usage from the electricity grid. The BIPV system is optimized using the Genetic Algorithm Optimization method in MATLAB. The output results include the optimal surface area used by BIPV module in east, west and …

Quantification Of Antiviral Drug Tenofovir (Tfv) By Surface-Enhanced Raman Spectroscopy (Sers) Using Cumulative Distribution Functions (Cdfs), Marguerite R. Butler, Jana Hrncirova, Meredith Clark, Sucharita Dutta, John B. Cooper

Chemistry & Biochemistry Faculty Publications

Surface-enhanced Raman spectroscopy (SERS) is an ultrasensitive spectroscopic technique that generates signal-enhanced fingerprint vibrational spectra of small molecules. However, without rigorous control of SERS substrate active sites, geometry, surface area, or surface functionality, SERS is notoriously irreproducible, complicating the consistent quantitative analysis of small molecules. While evaporatively prepared samples yield significant SERS enhancement resulting in lower detection limits, the distribution of these enhancements along the SERS surface is inherently stochastic. Acquiring spatially resolved SERS spectra of these dried surfaces, we have shown that this enhancement is governed by a power law as a function of analyte concentration. Consequently, by definition, …

Investigating The Regulation Of Epithelial-Mesenchymal Transition Through Fibronectin-Mediated Tgf-Β1 Mechanotransduction Using In Vitro Models Of Triple-Negative Breast Cancer, Michael M. Sofroniou

Theses and Dissertations

TGF-β1 is a potent regulator of epithelial-mesenchymal transtion (EMT), a cellular
transdifferentation process that promotes cellular motility and matrix remodeling, critical to tumorigenesis and metastasis. TGF-β1-induced EMT promotes the assembly of FN, which synergistically binds latent TGF-β1 to aid in its activation. The purpose of of this work is to further probe the effects of autocrine TGF-β1 signaling during EMT progression by utilizing CRISPR-Cas9 deletion of the TGFB1 gene in in vitro models of triple-negative breast cancer. The studies outlined in this work demonstrate that autocrine TGF-β1 maintains a baseline level of mesenchymal marker expression. It also functions to desensitize …

Nano-Patterned Si Structures For Optical Filters And Electro-Mechanical Relays: Fabrication, Characterization, Prospects, And Limitations, Md Ataul Mamun

Theses and Dissertations

Nanofabrication technology, especially nanopatterning, is a rapidly advancing field that has already resulted in creating novel devices and holds promise for producing even more with unmatched performance. These techniques also allow us to gain insight into physical phenomena at the micro- and nanoscale. The ultimate performance of nanofabricated devices and their compatibility with existing Si-based CMOS technology hinge upon the careful selection of materials and precise design, coordinated with meticulous pattern transfer. In this work, we applied nanopatterning techniques on silicon to create optical filters for the shortwave infrared (SWIR) region and nanoelectromechanical system (NEMS) relay-based logic gates. Additionally, these …

Adaptable And Trustworthy Machine Learning For Human Activity Recognition From Bioelectric Signals, Morgan S. Stuart

Theses and Dissertations

Enabling machines to learn measures of human activity from bioelectric signals has many applications in human-machine interaction and healthcare. However, labeled activity recognition datasets are costly to collect and highly varied, which challenges machine learning techniques that rely on large datasets. Furthermore, activity recognition in practice needs to account for user trust - models are motivated to enable interpretability, usability, and information privacy. The objective of this dissertation is to improve adaptability and trustworthiness of machine learning models for human activity recognition from bioelectric signals. We improve adaptability by developing pretraining techniques that initialize models for later specialization to unseen …

Insights Into The Characterization And Degradation Of Electrospun Polycaprolactone Scaffolds For Tissue Engineering Applications, Caleb B. Wells

Theses and Dissertations

Electrospun polymeric biodegradable scaffolds are essential in tissue engineering, particularly for Engineered Tissue Vascular Grafts (ETVGs), which promise advancements in treating coronary artery disease, peripheral arterial disease, congenital cardiovascular defects, and renal disease. These scaffolds present a solution to issues with autologous graft availability and durability. While large-diameter grafts in low-pressure environments have seen success, small-diameter grafts in high-flow scenarios remain challenging. Understanding polymeric scaffold degradation and behavior during incubation, especially under dynamic mechanical loading, is vital for clinical translation of small-caliber ETVGs.

This research focuses on characterizing the mechanical and microstructural properties of electrospun polycaprolactone (PCL) scaffolds and their …

Gender Classification Through Postural Analysis: A Comparative Study Of 2d Images And 3d Reconstructions., Prathamesh Dixit

Master's Projects

In computer vision, gender classification has become a vital task having applications in human-computer interaction, healthcare, and surveillance. In this study, we look at a two-step approach based on human joint information for gender classification. In this research, we use convolutional neural networks (CNNs).

With Leeds Sports Pose (LSP) dataset, we use a C5 pre-trained model to map and extract joint information from 2D RGB images and after pre-processing and background removal, we use PiFUHD to transform these 2D images into 3D representations. Next, we train our models on RGB images and joint images for both 2D and 3D representations. …

Developing Machine Learning Models For Selection Of Management Zones, Sravanthi Bachina

Electronic Theses and Dissertations

Soil sampling and analyses play a crucial role in optimizing nutrient management and enhancing crop productivity. However, collecting representative samples across diverse landscapes is challenging due to knowledge gaps about spatial variability of soil properties, large fields, multiple samples, and analysis costs. Collecting soil samples based on the management zones can help farmers gather precise information about soil properties with fewer samples. Recent developments in precision agriculture and machine learning. This study aimed to develop machine learning models that can learn, analyze, and refine landscape and soil properties data for automated selection of soil sampling zones and generating prediction maps. …

Mechanical Testing Of Novel Biopolymers For The Replacement Of Petroleum-Based Plastic Hay Bale Net Wrapping, Logan Wolf

Electronic Theses and Dissertations

The purpose of this thesis is to test biopolymers synthesized from oilseeds or produced from agricultural wastes for large bale net wrapping as a viable alternative to conventional petroleum-based plastics (PBP). The goal is to test and assess novel polymers to guide the synthesis of a benign biodegradable polymer. Biobased net wrapping will significantly reduce ingestion of PBPs in animals such as ruminants to improve digestive health and minimize cattle loss due to plastic disease. Biobased plastics will also achieve partial or complete breakdown shortly after service without depositing microplastics in the environment. This will reduce the risk of damage …

Climate Change Adaptation For Embankment Through Biogeotechnics, Debayan Ghosh

Electronic Theses and Dissertations

Problematic soil like expansive soil, and frost-susceptible soil causes significant damage to civil infrastructure. The use of calcium-based stabilizers in the treatment of sulfate-rich expansive soils is not suggested due to the formation of ettringite. Infrastructure such as pavements and embankments built on expansive soil are often exposed to the damaging impacts of freeze-thaw cycles in areas prone to seasonal freezing making them vulnerable to cracking and spalling. In this study, a native expansive soil from South Dakota with a sulfate content of more than 10,000 ppm was stabilized using biopolymer and cement. The experimental study investigated the strength and …

Investigating The Efficacy Of Natural And Nature-Based Features To Increase The Service Life Of Coastal Roadways, Navid Jafari, Mohammad O. Hassan

Data

Wetlands serve as essential natural barriers, protecting coastal regions against the increasing threats from hurricane-induced storm surges, rising sea levels, and changing weather patterns. Understanding their structural resilience is crucial, particularly the shear strength of the wetland soil-root matrix, which contributes significantly to stability under extreme weather events. However, evaluating this shear strength is challenging due to the complex nature of root systems and the varying amounts of live biomass and necromass at different soil depths. This study utilized a Large-scale Direct Shear Apparatus (LDSA) to address this gap by assessing the shear strength of root biomass in a horizontal …

Investigating The Efficacy Of Natural And Nature-Based Features To Increase The Service Life Of Coastal Roadways, Navid Jafari, Mohammad O. Hassan

Publications

Wetlands serve as essential natural barriers, protecting coastal regions against the increasing threats from hurricane-induced storm surges, rising sea levels, and changing weather patterns. Understanding their structural resilience is crucial, particularly the shear strength of the wetland soil-root matrix, which contributes significantly to stability under extreme weather events. However, evaluating this shear strength is challenging due to the complex nature of root systems and the varying amounts of live biomass and necromass at different soil depths. This study utilized a Large-scale Direct Shear Apparatus (LDSA) to address this gap by assessing the shear strength of root biomass in a horizontal …

Development Of Innovative Flocculation Technologies For Agricultural Water Treatment, Noor Haleem

Electronic Theses and Dissertations

The development of innovative flocculation technologies is essential for addressing the challenges of agricultural water treatment. These technologies play a crucial role in removing contaminants such as suspended solids and nutrients, thereby ensuring safer water for irrigation and livestock consumption. By enhancing water quality and wastewater management, they contribute significantly to environmental sustainability and public health in agricultural communities. This comprehensive thesis extensively explores various dimensions of flocculation, with a focused effort on methodologies and resources aimed at strengthening sustainability and efficiency. A pivotal aspect of the research involves synthesizing cationic starch (CS), a flocculant derived from an underutilized resource, …

Advancing Biological Applications Through Microfluidic-Based Tool Development, K.M. Taufiqur Rahman

Electronic Theses and Dissertations

This research undertook an interdisciplinary approach, integrating bioengineering, microbiology, molecular biology, and systems biology to investigate bacterial dynamics behavior. Specifically, it delved into the development of microfluidic devices for biological applications such as bacterial cell counts, real-time observation of plant roots (here, specialized lectin-coated microbeads are used that mimic root characteristics), and soil microbe interactions. Furthermore, Next-Generation Sequencing and systems biology methodologies were employed to explore the intricate, multifaceted survival mechanisms of Escherichia coli persister population. Studying and quantifying persisters or testing for the existence of VBNC (viable but nonculturable) is challenging. These experiments require precise counts. It has been …

Underwater Nitrate Monitoring With Wireless Flexible Sensor Networks, Shah Zayed Riam

Electrical Engineering Theses

This study introduces a sophisticated sensor array engineered for comprehensive monitoring of water quality, thereby addressing the pressing need for effective environmental conservation and water resource management. The array encompasses sensors for nitrate, pH, and temperature, seamlessly integrated into a unified flexible platform capable of wireless data transmission for real-time monitoring applications. Employing potentiometric detection methods, the nitrate and pH sensors offer robust analytical capabilities, while the temperature sensor operates on resistive principles.

The nitrate sensor, constructed using a novel nanocomposite comprising poly(3-octyl-thiophene) (PoT) and molybdenum disulfide (MoS₂), exhibits a sensitivity of -50 mV against every decade change …

Characterizing Human Schwann Cell Behavior Using Hyaluronic Acid Material Properties, Christine Hampton

Wayne State University Theses

Schwann cells are the principal glia in the peripheral nervous system, supporting normal peripheral nerve function, particularly in the myelination of motor and sensory axons of the peripheral nerves. Disruptions in normal Schwann cell function such as injury and disease can lead to decreased motor function, sensory capability, and neuropathic pain. Efforts to treat this do not always have consistent or favorable outcomes, so there has been an increased interest in developing tissue engineering solutions to address these problems. Studies have been conducted on various cell types to characterize the cellular response to material properties such as topography and material …

The Effect Of Phase-Separated Microstructures On Cell Migration In 3d Dextran Hydrogels, Jenifer Sidhwa

Honors Theses and Capstones

No abstract provided.

Tractor Test 2280: John Deere 9rt 490 Diesel, Nebraska Tractor Test Lab

Nebraska Tractor Tests

ABOUT THE TEST REPORT AND USE OF THE DATA The test data contained in this report are a tabulation of the results of a series of tests. Due to the restricted format of these pages, only a limited amount of data and not all of the tractor specifications are included. The full OECD report contains usually about 30 pages of data and specifications. The test data were obtained for each tractor under similar conditions and therefore, provide a means of comparison of performance based on a limited set of reported data. EXPLANATION OF THE TEST PROCEDURES Purpose The purpose of …

Tractor Test #2295: Kubota M7-174, Nebraska Tractor Test Lab

Nebraska Tractor Tests

ABOUT THE TEST REPORT AND USE OF THE DATA The test data contained in this report are a tabulation of the results of a series of tests. Due to the restricted format of these pages, only a limited amount of data and not all of the tractor specifications are included. The full OECD report contains usually about 30 pages of data and specifications. The test data were obtained for each tractor under similar conditions and therefore, provide a means of comparison of performance based on a limited set of reported data. EXPLANATION OF THE TEST PROCEDURES Purpose The purpose of …

Tractor Test #2293: Kubota M7-134, Nebraska Tractor Test Lab

Nebraska Tractor Tests

ABOUT THE TEST REPORT AND USE OF THE DATA The test data contained in this report are a tabulation of the results of a series of tests. Due to the restricted format of these pages, only a limited amount of data and not all of the tractor specifications are included. The full OECD report contains usually about 30 pages of data and specifications. The test data were obtained for each tractor under similar conditions and therefore, provide a means of comparison of performance based on a limited set of reported data. EXPLANATION OF THE TEST PROCEDURES Purpose The purpose of …

Tractor Test # 2285: Claas Axion 940, Nebraska Tractor Test Lab

Nebraska Tractor Tests

ABOUT THE TEST REPORT AND USE OF THE DATA The test data contained in this report are a tabulation of the results of a series of tests. Due to the restricted format of these pages, only a limited amount of data and not all of the tractor specifications are included. The full OECD report contains usually about 30 pages of data and specifications. The test data were obtained for each tractor under similar conditions and therefore, provide a means of comparison of performance based on a limited set of reported data. EXPLANATION OF THE TEST PROCEDURES Purpose The purpose of …

Tractor Test #2284: Claas Axion 930 Terra Trac, Nebraska Tractor Test Lab

Nebraska Tractor Tests

ABOUT THE TEST REPORT AND USE OF THE DATA The test data contained in this report are a tabulation of the results of a series of tests. Due to the restricted format of these pages, only a limited amount of data and not all of the tractor specifications are included. The full OECD report contains usually about 30 pages of data and specifications. The test data were obtained for each tractor under similar conditions and therefore, provide a means of comparison of performance based on a limited set of reported data. EXPLANATION OF THE TEST PROCEDURES Purpose The purpose of …