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Hydrologic Controls On Equilibrium Soil Depths, L. Nicótina, David G. Tarboton, Teklu K. Tesfa, A. Rinaldo Apr 2011

Hydrologic Controls On Equilibrium Soil Depths, L. Nicótina, David G. Tarboton, Teklu K. Tesfa, A. Rinaldo

Civil and Environmental Engineering Faculty Publications

This paper deals with modeling the mutual feedbacks between runoff production and geomorphological processes and attributes that lead to patterns of equilibrium soil depth. Our primary goal is an attempt to describe spatial patterns of soil depth resulting from long-term interactions between hydrologic forcings and soil production, erosion, and sediment transport processes under the framework of landscape dynamic equilibrium. Another goal is to set the premises for exploiting the role of soil depths in shaping the hydrologic response of a catchment. The relevance of the study stems from the massive improvement in hydrologic predictions for ungauged basins that would be …


Experimental Study Of Liquid Squeeze-Flow As It Relates To Human Voice Production, Daniel Victor Lo Forte Apr 2011

Experimental Study Of Liquid Squeeze-Flow As It Relates To Human Voice Production, Daniel Victor Lo Forte

Theses and Dissertations

Approximately 7.5 million people suffer from voice disorders in the United States. Previous studies indicate that the quality of the fluid layer that coats the vocal folds appears to be different for people with voice disorders than for people whose voice is considered normal. These studies suggest that the composition and/or physical properties of the fluid layer may contribute to voice disorders. Despite these findings, little research has been undertaken to investigate the role of the fluid layer on voice, and in almost all cases, the fluid layer is considered to be insignificant. The purpose of this research was to …


3d Functional Modeling Of Dbs Efficacy And Development Of Analytical Tools To Explore Functional Stn, Deepak Kumbhare Apr 2011

3d Functional Modeling Of Dbs Efficacy And Development Of Analytical Tools To Explore Functional Stn, Deepak Kumbhare

Theses and Dissertations

Introduction: Exploring the brain for optimal locations for deep brain stimulation (DBS) therapy is a challenging task, which can be facilitated by analysis of DBS efficacy in a large number of patients with Parkinson’s disease (PD). The Unified Parkinson's Disease Rating Scale (UPDRS) scores indicate the DBS efficacy of the corresponding stimulation location in a particular patient. The spatial distribution of these clinical scores can be used to construct a functional model which closely models the expected efficacy of stimulation in the region. Designs and Methods: In this study, different interpolation techniques were investigated that can appropriately model the DBS …


2011 - The Sixteenth Annual Symposium Of Student Scholars Apr 2011

2011 - The Sixteenth Annual Symposium Of Student Scholars

Symposium of Student Scholars Program Books

The full program book from the Sixteenth Annual Symposium of Student Scholars, held on April 26, 2011. Includes abstracts from the presentations and posters.


Transverse Permeability Of Fibrous Porous Media, Ali Tamayol, Majid Bahrami Apr 2011

Transverse Permeability Of Fibrous Porous Media, Ali Tamayol, Majid Bahrami

Department of Mechanical and Materials Engineering: Faculty Publications

In this study, the transverse permeability of fibrous porous media is studied both experimentally and theoretically. A scale analysis technique is employed for determining the transverse permeability of various fibrous matrices including square, staggered, and hexagonal arrangements of unidirectionally aligned fibers, as well as simple two-directional mats and simple cubic structures. In the present approach, the permeability is related to the porosity, fiber diameter, and tortuosity of the medium. In addition, the pressure drop in several samples of tube banks of different arrangements and metal foams are measured in the creeping flow regime. The pressure-drop results are then used to …


Economic And Environmental Sustainability Of Using Bio-Fuels For Small Nebraska Greenhouses, David M. Mabie Apr 2011

Economic And Environmental Sustainability Of Using Bio-Fuels For Small Nebraska Greenhouses, David M. Mabie

Department of Environmental Engineering: Theses and Other Student Research

The primary goal of this paper was to increase profitability in Nebraska greenhouses by using biomass fuels for heating instead of propane. Several different fuels were tested, including whole shelled corn, dry distiller’s grains pelletized, wood pellets and blends between each biomass. The main fuel focus was on whole shelled corn. Bomb calorimetry tests were performed on biomass fuels and their respective ashes. Several furnace and heat exchanger efficiency tests were performed, with cost effectiveness analysis for each fuel type. Emissions data was also collected for each fuel on carbon monoxide, carbon dioxide, nitrous oxides, sulfuric oxides, and particulate matter. …


Design And Modeling Of Infrastructure For Residential And Community Water Reuse, Shannon M. Killion Apr 2011

Design And Modeling Of Infrastructure For Residential And Community Water Reuse, Shannon M. Killion

Department of Environmental Engineering: Theses and Other Student Research

Water scarcity and deteriorating water infrastructure are of growing concern in the United States. The conventional methods of treating and transporting potable water and wastewater are being challenged as new technology creates opportunities for water reuse. Instead of simply replacing the current infrastructure for centralized treatment systems, alternatives such as dual distribution and decentralized treatment systems are being investigated as more sustainable alternatives.

Implementing dual distribution systems leads to benefits such as reducing the amount of water treated to potable standards and reducing freshwater withdrawals. A dual distribution system allows the non-potable demands to be shifted from the potable water …


Impacts Of The Species Elaeagnus Umbellate On The Soil And Water Quality Of The Pierce Cedar Creek Institute Ecosystem, Yacoub Aljobeh, Kristin Engerer Apr 2011

Impacts Of The Species Elaeagnus Umbellate On The Soil And Water Quality Of The Pierce Cedar Creek Institute Ecosystem, Yacoub Aljobeh, Kristin Engerer

Symposium on Research and Creative Expression (SORCE)

The species Elaeagnus umbellate, more commonly known as autumn olive, is a shrub that is invasive to the United States and indigenous to East Asia. Even though the autumn olive is not native to North America, it was able to thrive and adapt to the new environment by using its ability to fix nitrogen. Nitrogen-fixing is a process where plants intake molecular nitrogen from the atmosphere and convert it into other forms of nitrogen that can be used by the plants. One of the nitrogen-fixing by-products is nitrate. Excessive amounts of nitrate can easily leach from the plant’s root …


A Cost-Benefit Analysis Of A Campus Computing Grid, Preston M. Smith Apr 2011

A Cost-Benefit Analysis Of A Campus Computing Grid, Preston M. Smith

Purdue Polytechnic Masters Theses

Any major research institution has a substantial number of computer systems on its campus, often in the scale of tens of thousands. Given that a large amount of scientific computing is appropriate for execution in an opportunistic environment, a campus grid is an inexpensive way to build a powerful computational resource. What is missing, though, is a model for making an informed decision on the cost-effectives of a campus grid. In this thesis, the author describes a model for measuring the costs and benefits of building a campus computing resource based on the institution’s existing investment in computing hardware.

For …


Nanocomposite High Displacement Strain Gauges For Use In Human-Machine Interfaces: Applications In Hand Pose Determination, Thomas B. Calkins Apr 2011

Nanocomposite High Displacement Strain Gauges For Use In Human-Machine Interfaces: Applications In Hand Pose Determination, Thomas B. Calkins

Theses and Dissertations

Conductive nanocomposites are finding many uses as multi-functional materials. One recent development involves the creation of high displacement strain gauges, which have potential applications in a variety of engineering roles. The piezoresistive nature of the gauges makes possible their strain sensing capability. The intent of this research is to show that specific High Displacement Strain Gauges can successfully be used in one human-machine interface application that will demonstrate their potential for a range of other human-machine interface applications. This will be shown in the development of these sensors to accomplish hand pose determination. The flexible and inexpensive gauges are attached …


Modeling, Design, And Testing Of An Underwater Microactuation System Using A Standard Mems Foundry Process, Gregory L. Holst Apr 2011

Modeling, Design, And Testing Of An Underwater Microactuation System Using A Standard Mems Foundry Process, Gregory L. Holst

Theses and Dissertations

This work presents the modeling, design, and testing of an underwater microactuation system. It is composed of several thermomechanical in-plane microactuators (TIM) integrated with a ratchet system to provide long displacements and high forces to underwater microelectromechanical systems (MEMS). It is capable of actuating a 200µN load 110µm. It is a two-layer silicon MEMS device fabricated with a MEMS fabrication process, PolyMUMPS. This work also shows the development of an elliptic integral model to analyze the compliant fixed-guided beams in the TIM and gives new insight into the buckling behavior, reaction forces, and displacement of the beams. The derivation, verification, …


Construction And Use Of A Direct Calorimeter To Estimate Energy Expenditure In Hibernators, Mark S. Burger, Frank Van Breukelen Apr 2011

Construction And Use Of A Direct Calorimeter To Estimate Energy Expenditure In Hibernators, Mark S. Burger, Frank Van Breukelen

Festival of Communities: UG Symposium (Posters)

Metabolic rates have traditionally been estimated through indirect calorimetry (gas exchange or respirometry) due to its ease and availability. Oxygen consumption neglects the contributions of anaerobic metabolism, while direct calorimetry (heat production) estimates both anaerobic and aerobic metabolism. Walsberg and Hoffman (2005) report anaerobic metabolism may be more important to small rodents than was previously thought. During the non-steady state of mammalian hibernation, we hypothesize that anaerobic processes may play an even greater role in fueling metabolism. To address this question, we constructed a very sensitive calorimeter that utilizes the Seebeck effect from a thermoelectric element (Peltier device). We describe …


Event Program, Carl Reiber, Nicholle Booker Apr 2011

Event Program, Carl Reiber, Nicholle Booker

Festival of Communities: UG Symposium (Posters)

UNLV Undergraduates from all departments, programs and colleges participated in a campus-wide symposium on April 16, 2011. Undergraduate posters from all disciplines and also oral presentations of research activities, readings and other creative endeavors were exhibited throughout the festival.


Dual-Channel Imaging System For Singlet Oxygen And Photosensitizer For Pdt, Seonkyung Lee, Martin E. Isabelle, Kristin L. Gabally-Kinney, Brian W. Pogue, Steven J. Davis Apr 2011

Dual-Channel Imaging System For Singlet Oxygen And Photosensitizer For Pdt, Seonkyung Lee, Martin E. Isabelle, Kristin L. Gabally-Kinney, Brian W. Pogue, Steven J. Davis

Dartmouth Scholarship

A two-channel optical system has been developed to provide spatially resolved simultaneous imaging of singlet molecular oxygen (1O2) phosphorescence and photosensitizer (PS) fluorescence produced by the photodynamic process. The current imaging system uses a spectral discrimination method to differentiate the weak 1O2 phosphorescence that peaks near 1.27 μ m from PS fluorescence that also occurs in this spectral region. The detection limit of 1O2 emission was determined at a concentration of 500 nM benzoporphyrin derivative monoacid (BPD) in tissue-like phantoms, and these signals observed were proportional to the PS fluorescence. Preliminary in …


Biodeterioration Of Coastal Concrete Structures By Macro Algae - Ulva Fasciata, Stri Hari Babu Jayakumar, Rama Saravanane, Thirumalai Sundararajan Apr 2011

Biodeterioration Of Coastal Concrete Structures By Macro Algae - Ulva Fasciata, Stri Hari Babu Jayakumar, Rama Saravanane, Thirumalai Sundararajan

Journal of Marine Science and Technology–Taiwan

Puducherry is a coastal region in India where the growth of Ulva fasciata (Delile) is very abundant on all marine structures. Though the detrimental effect of this macro algae U. fasciata is a secondary one, its effect has to be investigated. To know its effect, the basic mechanism by which U. fasciata deteriorates concrete structures has been highlighted. To ascertain its detrimental effect on coastal concrete structures, M20 grade concrete cubes were casted and immersed in the coastal area where there is abundant growth of U. fasciata. In addition laboratory simulation has been carried out. Concrete samples collected from the …


Offline Optimization Of Advance Reservation Of Bandwidth Over Dynamic Circuit Networks, Pragatheeswaran Angu Apr 2011

Offline Optimization Of Advance Reservation Of Bandwidth Over Dynamic Circuit Networks, Pragatheeswaran Angu

School of Computing: Dissertations, Theses, and Student Research

E-science projects require very high-speed and reliable networks to transfer data across various destinations in the world. Dynamic Circuit Network (DCN) is a networking service to make advance reservation of bandwidth between a source and a destination in a network. In this thesis we solve the problem of advance reservation of bandwidth in next-generation wavelength-division multiplexing (WDM) networks using a simulation based approach.
We implement a greedy algorithm and a genetic algorithm in parallel, in separate threads. The request for advance reservation is processed by both but the user gets the response only from the greedy algorithm. The genetic algorithm …


Partnership For International Research And Education In Microfluidic Technology With Applications In Point Of Care Diagnostic, Mohammed Faghri, Sigrid Berka, John Grandin, Thomas Mather, D.M.L Meyer, Zongqin Zhang, Otto Gregory, Stephan Grilli, Christopher Baxter, Constantine Anagnostopoulos, Assem Abolmaaty Apr 2011

Partnership For International Research And Education In Microfluidic Technology With Applications In Point Of Care Diagnostic, Mohammed Faghri, Sigrid Berka, John Grandin, Thomas Mather, D.M.L Meyer, Zongqin Zhang, Otto Gregory, Stephan Grilli, Christopher Baxter, Constantine Anagnostopoulos, Assem Abolmaaty

Discovery@URI

This poster summarizes the research highlights of a project conducted as part of an National Science Foundation (NSF) partnership for research and education. The objective of this multidisciplinary, international project was to conduct research on microfluidic technology and applications. The project team is comprised of participants from the University of Rhode Island and the Technical University of Braunschweig in Germany. The research focuses on the following four tasks: Task 1 – Discovery of disease biomarkers; Task 2 –Streaming based microfluidic platform for pumping, mixing, separation and detection; Task 3 – Development of rapid, quantitative and sensitive microfluidic fluorescence immunosensors for …


Development Of High Resolution Sea Floor Mapping Tools And Techniques, Gabrielle Inglis, J. Ian Vaughn, Clara Smart, Christopher N. Roman Apr 2011

Development Of High Resolution Sea Floor Mapping Tools And Techniques, Gabrielle Inglis, J. Ian Vaughn, Clara Smart, Christopher N. Roman

Graduate School of Oceanography Faculty Publications

There is a persistent need for high resolution photographic and bathymetric maps of the sea floor for many research areas in marine geology, biology and archaeology. This poster will present recent work using high frequency multibeam sonars, stereo vision and structured light laser imaging techniques to create maps with centimeter resolution for these applications. This research involves the development of new image and sonar processing techniques that combat the typical difficulties of imperfect navigation information, limited sensor ranges and adverse environmental conditions associated with using marine robotic vehicles in the ocean. Data for this work has been collected with the …


Mitochondrial Approaches To Protect Against Cardiac Ischemia And Reperfusion Injury, Amadou K.S. Camara, Martin Bienengraeber, David F. Stowe Apr 2011

Mitochondrial Approaches To Protect Against Cardiac Ischemia And Reperfusion Injury, Amadou K.S. Camara, Martin Bienengraeber, David F. Stowe

Biomedical Engineering Faculty Research and Publications

The mitochondrion is a vital component in cellular energy metabolism and intracellular signaling processes. Mitochondria are involved in a myriad of complex signaling cascades regulating cell death vs. survival. Importantly, mitochondrial dysfunction and the resulting oxidative and nitrosative stress are central in the pathogenesis of numerous human maladies including cardiovascular diseases, neurodegenerative diseases, diabetes, and retinal diseases, many of which are related. This review will examine the emerging understanding of the role of mitochondria in the etiology and progression of cardiovascular diseases and will explore potential therapeutic benefits of targeting the organelle in attenuating the disease process. Indeed, recent advances …


Bifurcation-Based Mass Sensing Using Piezoelectrically-Actuated Microcantilevers, Vijay Kumar, J. William Boley, Yushi Yang, Hendrik Ekowaluyo, Jacob K. Miller, George T.C. Chiu, Jeff F. Rhoads Apr 2011

Bifurcation-Based Mass Sensing Using Piezoelectrically-Actuated Microcantilevers, Vijay Kumar, J. William Boley, Yushi Yang, Hendrik Ekowaluyo, Jacob K. Miller, George T.C. Chiu, Jeff F. Rhoads

Birck and NCN Publications

In conventional implementations, resonant chemical and biological sensors exploit chemomechanically-induced frequency shifts, which occur in linear systems, for analyte detection. In this letter, an alternative sensing approach, based upon dynamic transitions across saddle-node bifurcations is investigated. This technique not only has the potential to render improved sensor metrics but also to eliminate frequency tracking components from final device implementations. The present work details proof-of-concept experiments on bifurcation-based sensing, which were conducted using selectively functionalized, piezoelectrically-actuated microcantilevers. Preliminary results reveal the proposed sensing technique to be a viable alternative to existing resonant sensing methods. (C) 2011 American Institute of Physics. [doi:10.1063/1.3574920]


Gas Embolic Factors In Cardiovascular Health, Eric L. Cutler Apr 2011

Gas Embolic Factors In Cardiovascular Health, Eric L. Cutler

Department of Mechanical and Materials Engineering: Dissertations, Theses, and Student Research

Gas emboli are a clinical issue often encountered in the space exploration, marine construction, and medical fields.  Individuals involved with these disciplines will often have asympomatic gas emboli circulating throughout their bodies on a regular basis.  A study into the impact of chronic asymptomatic gas emboli on the risk of atherosclerosis in humans is thus presented.  This research utilized a custom built cardiovascular flow simulator and accompanying data acquisition system to experimentally determine the influence of gas emboli under varied circumstances on the mean wall shear stress of a tube in-vitro.  A directly inverse relationship between gas embolus presence in …


Quantitative Reappraisal Of The Helmholtz-Guyton Resonance Theory Of Frequency Tuning In The Cochlea, Charles F. Babbs Apr 2011

Quantitative Reappraisal Of The Helmholtz-Guyton Resonance Theory Of Frequency Tuning In The Cochlea, Charles F. Babbs

Weldon School of Biomedical Engineering Faculty Publications

To explore the fundamental biomechanics of sound frequency transduction in the cochlea, a two-dimensional analytical model of the basilar membrane was constructed from first principles. Quantitative analysis showed that axial forces along the membrane are negligible, condensing the problem to a set of ordered one-dimensional models in the radial dimension, for which all parameters can be specified from experimental data. Solutions of the radial models for asymmetrical boundary conditions produce realistic deformation patterns. The resulting second-order differential equations, based on the original concepts of Helmholtz and Guyton, and including viscoelastic restoring forces, predict a frequency map and amplitudes of deflections …


Wright State University Celebration Of Research, Scholarship And Creative Activities Book Of Abstracts Friday, April 8, 2011, Wright State University Office Of Undergraduate Research And Stemm Activities Apr 2011

Wright State University Celebration Of Research, Scholarship And Creative Activities Book Of Abstracts Friday, April 8, 2011, Wright State University Office Of Undergraduate Research And Stemm Activities

Celebration of Undergraduate & Graduate Research, Scholarship, and Creative Activities Materials

The student abstract booklet is a compilation of abstracts from students' oral and poster presentations at Wright State University's second annual Celebration of Research, Scholarship and Creative Activities on April 8, 2011.


Accelerating Finite State Projection Through General Purpose Graphics Processing, Thomas Trimeloni Apr 2011

Accelerating Finite State Projection Through General Purpose Graphics Processing, Thomas Trimeloni

Theses and Dissertations

The finite state projection algorithm provides modelers a new way of directly solving the chemical master equation. The algorithm utilizes the matrix exponential function, and so the algorithm’s performance suffers when it is applied to large problems. Other work has been done to reduce the size of the exponentiation through mathematical simplifications, but efficiently exponentiating a large matrix has not been explored. This work explores implementing the finite state projection algorithm on several different high-performance computing platforms as a means of efficiently calculating the matrix exponential function for large systems. This work finds that general purpose graphics processing can accelerate …


Capture And Sequestration Of Carbon Dioxide In Flue Gases, Surendra Komar Kawatra, Timothy C. Eisele, John J. Simmons Apr 2011

Capture And Sequestration Of Carbon Dioxide In Flue Gases, Surendra Komar Kawatra, Timothy C. Eisele, John J. Simmons

Michigan Tech Patents

There is provided a process for the capture and sequestration of carbon dioxide that would otherwise enter the atmosphere and contribute to global warming and other problems. C02 capture is accomplished by reacting carbon dioxide in flue gas with an alkali metal carbonate, or a metal oxide, particularly containing an alkaline earth metal or iron, to form a carbonate salt. A preferred carbonate for C02 capture is a dilute aqueous solution of additive-free (Na2C03). Other carbonates include (K2C03) or other metal ion that can produce both a carbonate and a bicarbonate salt. Examples of suitable metal oxides include several alkaline …


Single Molecule In Vivo Analysis Of Toll-Like Receptor 9 And Cpg Dna Interaction, Jiji Chen, Suman Nag, Pierre-Alexandre Vidi, Joseph Irudayaraj Apr 2011

Single Molecule In Vivo Analysis Of Toll-Like Receptor 9 And Cpg Dna Interaction, Jiji Chen, Suman Nag, Pierre-Alexandre Vidi, Joseph Irudayaraj

Birck and NCN Publications

Toll-like receptor 9 (TLR9) activates the innate immune system in response to oligonucleotides rich in CpG whereas DNA lacking CpG could inhibit its activation. However, the mechanism of how TLR9 interacts with nucleic acid and becomes activated in live cells is not well understood. Here, we report on the successful implementation of single molecule tools, constituting fluorescence correlation/cross-correlation spectroscopy (FCS and FCCS) and photon count histogram (PCH) with fluorescence lifetime imaging (FLIM) to study the interaction of TLR9-GFP with Cy5 labeled oligonucleotide containing CpG or lacking CpG in live HEK 293 cells. Our findings show that i) TLR9 predominantly forms …


An Integrated Circuit/Microsystem/Nano-Enhanced Four Species Radiation Sensor For Inexpensive Fissionable Material Detection, Randy Paul Waguespack Apr 2011

An Integrated Circuit/Microsystem/Nano-Enhanced Four Species Radiation Sensor For Inexpensive Fissionable Material Detection, Randy Paul Waguespack

Doctoral Dissertations

Small scale radiation detectors sensitive to alpha, beta, electromagnetic, neutron radiation are needed to combat the threat of nuclear terrorism and maintain national security. There are many types of radiation detectors on the market, and the type of detector chosen is usually determined by the type of particle to be detected. In the case of fissionable material, an ideal detector needs to detect all four types of radiation, which is not the focus of many detectors. For fissionable materials, the two main types of radiation that must be detected are gamma rays and neutrons. Our detector uses a glass or …


Manipulating Cellular Growth Responses To Patterning, Apoptotic, And Environmental Cues, James N. Mcnamara Apr 2011

Manipulating Cellular Growth Responses To Patterning, Apoptotic, And Environmental Cues, James N. Mcnamara

Doctoral Dissertations

In this work, various methods of controlling cell growth are examined. Cell-cell interaction, apoptotic cues, three dimensional scaffolds, and non-adherent environments are evaluated for their ability to affect the differentiation, morphology, and growth rate of different cell types. Previous work has shown that cell growth and cell morphology can be influenced by patterns of polymers coated on surfaces in two dimensions, designated here as the x and y dimensions of a standard Cartesian coordinate system. Tissue engineering and regenerative medicine studies have shown limited success in modifying growth in the third, z dimension. This work considers not only the x, …


Development Of Approximating Functions To Model And Predict The Properties Of Fresh And Hardened Fly Ash-Based Geopolymer Concrete, Eleazar Ivan Diaz Loya Apr 2011

Development Of Approximating Functions To Model And Predict The Properties Of Fresh And Hardened Fly Ash-Based Geopolymer Concrete, Eleazar Ivan Diaz Loya

Doctoral Dissertations

The manuscript presented herein is based on the investigation of the mechanical properties of fly ash-based geopolymer concrete and their link to fly ash (FA) characteristics. A database of 32 FA samples was created. Each FA sample was analyzed in terms of chemical composition, crystallographic properties and particle size distribution. The mechanical performance of geopolymer concrete (GPC) made from each FA sample was evaluated in terms of density, setting time, compressive and flexural strength, static elastic modulus and Poisson's ratio. It is worth mentioning that the author has already published preliminary results of this study (Diaz and Allouche, 2010; Diaz …


De Novo Gene Synthesis By Rapid Polymerase Chain Assembly Coupled With Immunoaffinity Purification: A Novel Process And Workstation, Joel R. Termaat Apr 2011

De Novo Gene Synthesis By Rapid Polymerase Chain Assembly Coupled With Immunoaffinity Purification: A Novel Process And Workstation, Joel R. Termaat

Department of Chemical and Biomolecular Engineering: Dissertations, Theses, and Student Research

The de novo synthesis of genes is emerging as a powerful tool in biotechnology. The ability to synthesize genes of any desired sequence opens the door to seemingly unlimited research possibilities. Major advances have been made recently in de novo gene synthesis, with Polymerase Chain Assembly (PCA) routinely used to construct functional sequences from short single-stranded oligonucleotides. However, current PCA techniques are lacking in speed and fidelity. Additionally, substantial undesired reactants/products are present in the final reaction. A novel process and accompanying workstation has been developed that incorporates rapid PCA synthesis coupled with subsequent affinity purification of the synthesis mixture. …