Digital Commons Network^™

A Morphological Analysis Of A Hybrid Swarm Of Native Ulmus Rubra And Introduced U. Pumila (Ulmaceae) In Southeastern Nebraska, Christian Elowsky, Ingrid E. Jordon-Thaden, Robert B. Kaul

Nebraska Center for Biotechnology: Faculty and Staff Publications

The parental species and hybrid swarm of native Ulmus rubra Muhl. and the introduced, naturalized and weedy U. pumila L. were investigated in a 65-km transect in Lancaster, Saunders, and Butler counties in Nebraska. Thirty-two trees of U. rubra, 32 of U. pumila, and 50 of the hybrid swarm were sampled for leaves and buds and subsampled for flowers and fruits. Leaves were measured for petiole length, blade length, width, primary and secondary teeth per cm, number of secondary teeth per primary tooth, and texture. Buds were scored for color and distribution of trichomes. Flowers were sampled for stamen counts …

The Effects Of Sensor Performance As Modeled By Signal Detection Theory On The Performance Of Reinforcement Learning In A Target Acquisition Task, Nate Quirion

Doctoral Dissertations and Master's Theses

Unmanned Aerial Systems (UASs) today are fulfilling more roles than ever before. There is a general push to have these systems feature more advanced autonomous capabilities in the near future. To achieve autonomous behavior requires some unique approaches to control and decision making. More advanced versions of these approaches are able to adapt their own behavior and examine their past experiences to increase their future mission performance. To achieve adaptive behavior and decision making capabilities this study used Reinforcement Learning algorithms. In this research the effects of sensor performance, as modeled through Signal Detection Theory (SDT), on the ability of …

Investigating The Possible Sources Of Error Using The Method Of Moments To Solve A Dielectric Scattering Problem, Phillip D. Bishop

Master's Theses (2009 -)

In an electromagnetic scattering problem, an incoming electromagnetic wave interacts with an object. The object is typically located in some medium, such as free space. When this electromagnetic wave becomes incident upon the object, the wave scatters. The goal of this work is to analyze the scattered fields for three different incoming wave types: a plane wave, a monopole line source, and a multipole line source. Each source is incident on an infinitely long circular cylinder of lossless dielectric material. Each source has a unique scattering characteristic.

The volume equivalence principle is used to replace the object geometry with mathematically …

Resonant Characteristics Of Rectangular Hammerhead Microcantilevers Vibrating Laterally In Viscous Liquid Media, Jinjin Zhang, Fabien Josse, Stephen M. Heinrich, Nicholas J. Nigro, Isabelle Dufour, Oliver Brand

Civil and Environmental Engineering Faculty Research and Publications

The resonant characteristics of laterally vibrating rectangular hammerhead microcantilevers in viscous liquid media are investigated. The rectangular hammerhead microcantilever is modeled as an Euler-Bernoulli beam (stem) and a rigid body (head). A modified semi-analytical expression for the hydrodynamic function in terms of the Reynolds number, Re, and aspect ratio, h/b, is proposed to rapidly evaluate the sensing characteristics. Using this expression, the resonance frequency, quality factor and normalized surface mass sensitivity are investigated as a function of the dimensions of the microcantilever and liquid properties. Guidelines for design of hammerhead microcantilever geometry are proposed to achieve efficient sensing platforms for …

Microwave Assisted Reconstruction Of Optical Interferograms For Distributed Fiber Optic Sensing, Jie Huang, Lei Hua, Xinwei Lan, Tao Wei, Hai Xiao

Electrical and Computer Engineering Faculty Research & Creative Works

This paper reports a distributed fiber optic sensing technique through microwave assisted separation and reconstruction of optical interferograms in spectrum domain. The approach involves sending a microwave-modulated optical signal through cascaded fiber optic interferometers. The microwave signal was used to resolve the position and reflectivity of each sensor along the optical fiber. By sweeping the optical wavelength and detecting the modulation signal, the optical spectrum of each sensor can be reconstructed. Three cascaded fiber optic extrinsic Fabry-Perot interferometric sensors were used to prove the concept. Their microwave-reconstructed interferogram matched well with those recorded individually using an optical spectrum analyzer. The …

Why A 15 Minute Biological Detection System?, Doug Lewis

Faculty Publications

The biological defense community needs to take a hard look at the requirements and assumptions we use to develop our biological sensors. Today the point sensors currently deployed or in development can offer at best “near real time” detection. This translates into approximately 10-20 minutes from the time an agent passes over the device until an alarm is issued. Why are we working to develop detection hardware which in reality contributes little to no advantage to an operational environment? Should the DOD resist fielding (in the near term) “near real time” detection systems, and instead field slower (but much more …

Inviscid Analysis Of Extended Formation Flight, James Kless, Michael Aftosmis, Andrew Ning, Marian Nemec

Faculty Publications

Flying airplanes in extended formations, with separation distances of tens of wingspans, significantly improves safety while maintaining most of the fuel savings achieved in close formations. The present study investigates the impact of roll trim and compressibility at a fixed lift coefficient on the benefits of extended formation flight. An Euler solver with adjoint-based mesh refinement combined with a wake propagation model is used to analyze a two-body echelon formation at a separation distance of 30 spans. Two geometries are examined: a simple wing and a wing-body geometry. Energy savings, quantified by both formation drag fraction and span efficiency factor, …

Protein Loop Length Estimation From Medium Resolution Cryoem Images, Andrew R. Mcknight

Computer Science Theses & Dissertations

In the post-genomic era, proteomics research presents a new frontier in life science. Proteins play roles in virtually every biological process, and understanding their atomic structures is the key to unraveling how they carry out their work. Compared to the over half million protein sequences in UniProt, only around 25,000 unique sequences have been atomically modeled and deposited to PDB (Protein Databank). Cryoelectron Microscopy (cryoEM) is an important biophysical technique that produces 3D subnanometer resolution images of molecules not amenable to past approaches like x-ray crystallography or nuclear magnetic resonance. De novo modeling is becoming a promising approach to derive …

New Types Of Morpho-Physiological Changes In Cells Exposed To Nanosecond Pulsed Electric Field, Mikhail A. Rassokhin

Theses and Dissertations in Biomedical Sciences

Exposure of cells to a pulsed electric field (PEF) is the basis of multiple techniques and treatments. Nanosecond pulsed electric field (nsPEF) poses unique characteristics to induce subtle cellular effects while preserving cell integrity. Improving understanding of the mechanisms triggered by nsPEF in cells inspires new applications for the nanosecond pulse technology. Although many effects of nsPEF remain unknown, they can be inferred from morpho-physiologic changes, or cell reshaping, that accompany nsPEF exposure. During the exposure cells undergo reshaping that is manifested in swelling and diffuse blebbing. Recently we identified two new distinct forms of reshaping, pseudopod-like blebbing and microvesiculation, …

The Rq-Tech Methodology: A New Paradigm For Conceptualizing Strategic Enterprise Architectures, Christine Ann Hoyland

Engineering Management & Systems Engineering Theses & Dissertations

The purpose of this research is to develop and apply a system-theoretic based methodology and corresponding model for Enterprise Architecture development. Enterprise Architectures can assist managers by illustrating the systemic relationships within an organization and the impact changes to the organization could make. Unfortunately, today's modeling practices are proprietary, time-consuming, and generally ineffective as tools for understanding the consequences of strategic-level planning decisions across all levels of the enterprise. This research supports the conclusion that system-specific solutions produce islands of technology and can be prevented by employing better enterprise change planning.

This research combined the practice of Enterprise Architectures with …

Isolation And Characterisation Of Micro-Organisms With Industrial Importance From Sisal Bole Rots, Consolatha Mhaiki

Tanzania Journal of Engineering and Technology (TJET)

Investigation of microorganisms naturally acclimatized to Agave hybrid H 11648 (sisal bole rot) was conducted, with the aim of isolating and characterizing Aspergillus niger strains for industrial use. Microorganism were identified morphologically and then confirmation made by polymerase chain reaction (PCR). Results showed the existence of four major groups, listed in order of abundances as follows; Aspergilli (36.0±0.8) %, Penicillin (28.0±0.1) %, Yeast (15.0±1.6) %and Fusarium (10.0±0.12) %. The main groups of Aspergilli strains were A. nidulans, A. tamari and A. niger in ratios (3:2:2), respectively. Several endo-spore forming non-enteric gram (-) rods and coccid bacteria identified by API 20 …

Problems And Prospects Of Hydraulic Modelling For Environmental Flows Assessment Studies In East Africa, Preksedis Ndomba

Tanzania Journal of Engineering and Technology (TJET)

The objective of this paper is to document the problems and prospects of hydraulic modelling for Environmental Flows Assessment (EFA) studies based on selected case studies. Most of studies in East Africa use Holistic methodologies. An ideal data set for defining river hydraulics for most of these methods would be six data points of stage measurements over a good distribution of discharges, the stage of zero discharge and some flood-related data. Besides, in East African region EFA studies suffer from data scarcity (i.e., poorly gauged sites) and limited expertise and funding. The hydraulics studies conducted by the authors entailed desktop …

Electrochemical Nanofabrication Using Polyacrylamide Hydrogel As Soft Stamps, Wen Sun, Hai-Tao Fan, Da-Xiao Zhang, Dong-Jie Hu, Yong-Liang Zhou

Journal of Electrochemistry

The fabrication resolution of electrochemical wet stamping (E-WETS) was enhanced to several hundred nanometers from several ten micrometers by using polycarylamide gel (PAG) as soft stamps and through optimized processes. The PAG stamp was cured on a nanopatterned soft mold and soaked with 0.2 mol·L^-1 KCl solution, then contacted with a silicon wafer with gold film and applied with electric field. And the gold nanopatterns were achieved by the selective anodic dissolving, the diameter of fabricated gold spot was around 200 nm. The parameters which affected the fabrication processes have been discussed in detail.

Verifying Performance For Autonomous Robot Missions With Uncertainty, Damian M. Lyons, Ronald Arkin, Tsung-Ming Liu, Shu Jiang, Paramesh Nirmal

Faculty Publications

Establishing performance guarantees for robot missions is especially important for C-WMD applications. Software verification techniques, such as model checking (Clark 1999, Jhala & Majumdar 2009), can be applied to robotic applications but characteristics of this application area, including addition of a robot environment model and handling continuous spatial location well, exacerbate state explosion, a key weakness of these methods. We have proposed an approach to verifying robot missions that shifts the focus from state-based analysis onto the solution of a set of flow equations (Lyons et al. 2012). The key novelty introduced in this paper is a probabilistic spatial representation …

Developing The Confocal And Fluorescence Microscopy Techniques To Visualize The Eliposomes Inside Of Cells To Treat Cancer, Tara Pandey, Dr. William Pitt

Journal of Undergraduate Research

Cancer is among the leading causes of death in the world. The goal for this project was to visualize a liposome containing perfluorocarbon emulsion an eLiposome, inside of tumor cells using confocal and fluorescence microscopes. A successful study of this project would help to understand the behavior of cancer cells in the presence of drugs during targeted drug delivery for cancer.

Beyond Jee: Finding Publication Venues To Get Your Message To The ‘Right’ Audience, Amy S. Van Epps

Libraries Faculty and Staff Scholarship and Research

Very few publications exist outlining the different publishing opportunities within engineering education as a discipline. Most researchers think immediately of the Journal of Engineering Education (JEE), and as the primary publication associated with the American Society for Engineering Education it is a logical first thought.

Questions arise with new graduate students and young faculty who are trying to learn the scope of engineering education as a research discipline and need to identify possible publication venues. A broad list of publications has been generated and is maintained by the ASEE Student Division in cooperation with the Center for Engineering Learning and …

Apparatus And Method For Growing Biological Organisms For Fuel And Other Purposes, David J. Bayless, Morgan Lefay Vis-Chiasson, Ben J. Stuart, Gregory G. Kremer

Mechanical and Aerospace Engineering Faculty Research & Creative Works

A bioreactor apparatus in which a container has sidewalls, a floor and a ceiling defining a chamber that contains a slurry of water, nutrients and photosynthetic microorganisms. A plurality of optical fibers, each of which has a first end disposed outside the chamber and a second end in the mixture. A light collector spaced from the container has light incident on it and focuses the light onto the first ends of the plurality of optical fibers, thereby permitting the light to be conveyed into the mixture to promote photosynthesis. At least one nozzle is in fluid communication with a source …

Optical Characterization Of Optofluidic Waveguides Using Scattered Light Imaging, Micah Jenkins, Dr. Aaron Hawkins

Journal of Undergraduate Research

One exciting field of research within optics involves using on chip optical waveguides as biological sensors. Dr. Aaron Hawkins and his team of students have worked to develop a platform for bio-sensing using ARROW’s (anti-resonant reflecting optical waveguides) to confine light to a hollow core channel. These devices are filled with sample solutions and can be used to detect changes in optical transmission as fluorescently tagged particles traverse the hollow core channel.

Tapered Photoresist In The Manufacture Of Nanosieves, Meagan Bracken, Dr. Aaron Hawkins

Journal of Undergraduate Research

The purpose of this project was to assist in the manufacturing of nanosieves. The device is designed to separates nano-size particles from the fluid substance in which they are contained. An example of this, and one of the main targets of this project, is the separation of HDL and LDL cholesterol particles from a sample of blood. This goal of separating cholesterol has many benefits to the medical world. By creating a method to characterize HDL cholesterol sizes and concentrations we can monitor a person’s risk for heart disease. Because cholesterol particles have this important attribute of size characterization, it …

Cellular Automata In Cryptographic Random Generators, Jason Spencer

Technical Reports

Cryptographic schemes using one-dimensional, three-neighbor cellular automata as a primitive have been put forth since at least 1985. Early results showed good statistical pseudorandomness, and the simplicity of their construction made them a natural candidate for use in cryptographic applications. Since those early days of cellular automata, research in the field of cryptography has developed a set of tools which allow designers to prove a particular scheme to be as hard as solving an instance of a well-studied problem, suggesting a level of security for the scheme. However, little or no literature is available on whether these cellular automata can …

Selective Nanoparticles For Antimicrobial Therapies And Mri Diagnostics, Mark Andrew Hoppens

LSU Master's Theses

Early diagnosis and treatment of an infection and the selectivity of the treatment method are three parameters, which if optimized will greatly enhance a patient’s prognosis. Thus these three components have been, and continue to be extensively studied. Advances in biosynthesis and nanofabriciation have provided researchers with new tools with which to improve diagnostic and therapeutic techniques. Of these, inorganic nanoparticles (NPs) have shown great promise. Metallic nanoparticles have been demonstrated to successfully serve as antimicrobials, platforms for the transportation of therapeutic molecules, CT and MRI contrast agents, and thermal ablation. The recent paradigm of theranostics proposes substances that serve …

Spatiotemporal Fluorescent Detection Measurements Using Embedded Waveguide Sensors, Mark C. Harrison, Andrea M. Armani

Engineering Faculty Articles and Research

Integrated waveguide biosensors, when combined with fluorescent labeling, have significantly impacted the field of biodetection. While there are numerous types of waveguide sensors, the fundamental excitation method is fairly consistent: the evanescent field of the waveguide excites a fluorophore whose emission is detected, either directly via imaging or indirectly via a decrease in power transfer. Recently, a sensor device was demonstrated which is able to back-couple the emitted light into the waveguide, allowing the signal to be detected directly. However, this previous work focused on the development of an empirical model, leaving many theoretical questions unanswered. Additionally, the results from …

Design, Fabrication, And Analysis Of A 3u Cubesat Platform, Todd Chun-Van Osdol, Charles Dorsey, Jake Hedlund, Thomas Hoye, Owen Jacobs, Kadja Klarreich-Giglio, Elliott Martin, Michael Ruiz, Michael Schlesselmann, Zachary Singh

Mechanical Engineering Senior Theses

The goal of this project is to develop a 3U CubeSat nanosatellite bus capable of short duration technology verification experiments while providing compelling handson student education opportunities. This 3U CubeSat platform has been prototyped by the design team in order to demonstrate its capabilities. This spacecraft, the Unspecified Payload Active Attitude Control Nanosatellite (UPAACN), is designed to perform a 3-6 month technology verification experiment for a NASA test component and an SCU single-axis attitude control system (AACS) in Low Earth Orbit (LEO). This project builds upon the flight heritage of SCU's Robotic Systems Laboratory (RSL) and improves its satellite development …

Conference Program, Engineering Conferences International

BioEnergy IV: Innovations in Biomass Conversion for Heat, Power, Fuels and Chemicals

No abstract provided.

Amperometric Detection Of Bioamines In Cancer Borealis Using Microchip Capillary Electrophoresis Integrated With Micellar Chromatography, Ajay Fernandez, Jason Howard, Christina Shuh

Bioengineering Senior Theses

In order to study neuromodulation in Cancer borealis, commonly known as the Jonah crab, we designed a microchip capillary electrophoretic (CE) device with micellar electrokinetic chromatography (MEKC) to separate bioamines and detect their concentrations using amperometric detection (AD). The analysis will be used to characterize the bioamines: dopamine, octopamine, tyramine, serotonin, norepinephrine, and gammaaminobutyric acid (GABA). The device measures the concentration of bioamines in microdialysate samples taken directly from the pericardial cavity of the crab. The research on Jonah crabs will be applied to enhance our understanding of the crab neurology. Since the structure and in some instances, the function …

Effect Of Particle Size On Enzymatic Hydrolysis Of Pretreated Miscanthus, Vijay Singh, Esha Khullar, Bruce Dien, Kent Rausch, M.E. Tumbleson

BioEnergy IV: Innovations in Biomass Conversion for Heat, Power, Fuels and Chemicals

No abstract provided.

Influence Of Media Composition On The Growth Rate Of Chlorella Vulgaris And Scenedesmus Acutus Utilized For Co2 Mitigation, Czarena L. Crofcheck, Xinyi E, Aubrey Shea, Michael D. Montross, Mark Crocker, Rodney Andrews

Biosystems and Agricultural Engineering Faculty Publications

Atmospheric carbon dioxide levels have increased since the industrial revolution due to increasing combustion of fossil fuels. One possible CO₂ mitigation strategy is the use of microalgae for mitigation of CO₂. This paper focuses on the influence of media composition on the growth rate of two microalgae strains, Chlorella vulgaris and Scenedesmus actus. A KNO₃ based medium was found to work better for Chlorella, while a urea based equivalent worked better for Scenedesmus. The urea based media investigated here resulted in growth similar to that found with previously reported KNO₃ based media. …

Enabling Fermentation Utilization Of Pyrolytic Substrates For The Production Of Biorenewable Fuels And Chemicals, Laura Jarboe, Zhiyou Wen, Robert Brown

BioEnergy IV: Innovations in Biomass Conversion for Heat, Power, Fuels and Chemicals

No abstract provided.

Metabolic Engineering Of Clostridium Cellulolyticum For Advanced Biofuel Production, Andrea Liedtke, Stefan Jennewein

BioEnergy IV: Innovations in Biomass Conversion for Heat, Power, Fuels and Chemicals

No abstract provided.

Preliminary Assessments Of Microalgae Direct Transesterification For Biodiesel Production, G. Oliveri, I. Gargano, R. Marotta, R. Andreozzi, A. Marzocchella

BioEnergy IV: Innovations in Biomass Conversion for Heat, Power, Fuels and Chemicals

No abstract provided.