Digital Commons Network^™

Effect Of Diafiltration On Preparation Of Fermented Mung Beans Concentrate As Probiotic Savory Flavor Through Ultrafiltration Membrane, Aspiyanto Aspiyanto, Agustine Susilowati

Makara Journal of Technology

Diafiltration by means of the ultrafiltration system of probiotic fermented Mung beans (Phaseolus radiatus L.) concentrate has been performed to reduce or eliminate salts and smaller impurities than the nominal cut-off of the membrane of 20,000 nominal weight cut-off (NWCO). These processes have been conducted as an attempt in order to get a probiotic product with organoleptic acceptability, composition, and the optimal total lactic acid bacteria (LAB) counts because the presence of salts will affect on the viability of LAB and the cell lysis of LAB and limit its utility in food products. Concentrate of probiotic mung beans was prepared …

A Realistic Technology And Engineering Assessment Of Algae Biofuel Production, T. J. Lundquist, I. C. Woertz, N. W. T. Quinn, J. R. Benemann

Civil and Environmental Engineering

This report assesses the economics of microalgae biofuels production through an analysis of five production scenarios. These scenarios, or cases, are based on technologies that currently exist or are expected to become available in the near-term, including raceway ponds for microalgae cultivation, bioflocculation for algae harvesting, and hexane for extraction of algae oil. Process flow diagrams, facility site layouts, and estimates for the capital and operations costs of each case were developed de novo. This report also reviews current and developing microalgae biofuel technologies for both oil and biogas production, provides an initial assessment of the US and California …

Surface Hydration: Principles And Applications Toward Low-Fouling/Nonfouling Biomaterials, Lingyan Liu, Jie Zheng

Chemical, Biomolecular, and Corrosion Engineering Faculty Research

Surface resistance to nonspecific protein adsorption, cell/bacterial adhesion, and biofilm formation is critical for the development and performance of biomedical and analytical devices. Significant needs and efforts have been made in the development of biocompatible and bioactive materials for antifouling surfaces, but much of the work retains an empirical flavor due to the complexity of experiments and the lack of robust theoretical models. In this review, two major classes of nonfouling materials (i.e. hydrophilic and zwitterionic materials) and associated basic nonfouling mechanisms and practical examples are discussed. Highly hydrated chemical groups with optimized physical properties of the surface, along with …

An Automated Phenotype-Driven Approach (Geneforce) For Refining Metabolic And Regulatory Models, Dipak Barua, Joonhoon Kim, Jennifer L. Reed

Chemical and Biochemical Engineering Faculty Research & Creative Works

Integrated constraint-based metabolic and regulatory models can accurately predict cellular growth phenotypes arising from genetic and environmental perturbations. Challenges in constructing such models involve the limited availability of information about transcription factor-gene target interactions and computational methods to quickly refine models based on additional datasets. In this study, we developed an algorithm, GeneForce, to identify incorrect regulatory rules and gene-protein-reaction associations in integrated metabolic and regulatory models. We applied the algorithm to refine integrated models of Escherichia coli and Salmonella typhimurium, and experimentally validated some of the algorithm's suggested refinements. The adjusted E. coli model showed improved accuracy …

A Novel Piezoelectric Device With Dual Functions Of Studying Biological Soft Tissues, Avin A. Jamadagni

Mechanical & Aerospace Engineering Theses & Dissertations

This thesis presents the design of a novel piezoelectric device with dual functions of studying soft biological tissues. The device consists of a central circular plate and a set of flexural beams. The flexural beams are partially covered with piezoelectric film for integrated transduction. It can function as a force sensor for measuring the mechanical behavior of a tissue sample and a load applier for mimicking physiological loads experienced by the tissue sample in vivo, while the mechanical behavior can be visually observed under a microscope simultaneously. By combining the classic theories in solid mechanics and piezoelectricity, the performance of …

Chemical Changes In Soil And Soil Solution After Calcium Silicate Addition To A Northern Hardwood Forest, Youngil Cho, Charles T. Driscoll, Chris E. Johnson, Thomas G. Siccama

Civil and Environmental Engineering - All Scholarship

Liming has been used to mitigate effects of acidic deposition in forest ecosystems. This study was designed to examine the effects of calcium (Ca) supply on the spatial patterns and the relations between soil and soil solution chemistry in a base-poor forest watershed. Watershed 1 at the Hubbard Brook Experimental Forest in New Hampshire, USA was experimentally treated with wollastonite (CaSiO₃) in October, 1999. Exchangeable Ca (Ex-Ca), soil pH_s (in 0.01 M CaCl₂), effective cation exchange capacity (CEC_e), and effective base saturation (BS_e) increased, while exchangeable acidity (Ex-Acid) decreased …

Quantification Of 99mTc-Duramycin Uptake Kinetics In The Area-At-Risk After Myocardial Ischemia And Reperfusion, Joseph Capacete

Master's Theses (2009 -)

Myocardial infarction (MI) is a condition in which blood supply to the heart is insufficient. MI is associated with two forms of cell death: apoptosis and necrosis. ^99mTc-duramycin (^99mTc-D) is a novel radiopharmaceutical that detects cell death by recognizing externalized phosphatidylethanolamine. The objective of this study was to develop a compartmental model for ^99mTc-D uptake kinetics in normal and infarct myocardium, and utilize this model to compare the uptake kinetics of ^99mTc-D in MI with that of another radiopharmaceutical, ^99mTc-C2A-GST.

MI was induced in rats which were then injected (i.v.) with ^99mTc-D. Rats …

Enhanced Anthocyanin Extraction From Red Cabbage Using Pulsed Electric Field Processing, Tanya K. Gachovska, David A. Cassada, Jeyamkondan Subbiah, Milford Hanna, Harshavardhan Thippareddi, Daniel D. Snow

Department of Agricultural and Biological Systems Engineering: Faculty Publications

This study was conducted to evaluate the effect of pulsed electric field (PEF) treatment on anthocyanin extraction from red cabbage using water as a solvent. Mashed cabbage was placed in a batch treatment chamber and subjected to PEF (2.5 kV/cm electric field strength; 15 μs pulse width and 50 pulses, specific energy 15.63 J/g). Extracted anthocyanin concentrations (16 to 889 μg/mL) were determined using HPLC. Heat and light stabilities of the control and PEF-treated samples, having approximately the same initial concentrations, were studied. PEF treatments enhanced total anthocyanin extraction in water from red cabbage by 2.15 times with a higher …

Effect Of Mechanical Stimulation On Mesenchymal Stem Cell Seeded Cartilage Constructs, Karin Wartella

Theses and Dissertations

Cartilage tissue engineered constructs using mesenchymal stem cells were stimulated with 3 different stimulation algorithms to achieve characteristics mimicking the superficial tangential zone of articular cartilage. The stimulation algorithm of both compression and tension without an offset had the best properties out of all the evaluated groups.

Case Study Of Cold Chain Logistics Business Of Third Party Logistics Company, Han Bao

World Maritime University Dissertations

No abstract provided.

Department Of Biological Systems Engineering Newsletter, Volume 6, Number 2, July 2010

BSE Department Magazine

No abstract provided.

Advanced Biofuels: Commercializing The Efficient Syngas-To-Ethanol Platform, Wes Bolsen

CO2 Summit: Technology and Opportunity

No abstract provided.

Mapping The Medical Device Development Process, Scott T. Ham

Industrial Technology and Packaging

This project examined the use of process mapping as a tool to show the process of developing medical devices from a broad perspective that includes research, innovation, development, regulation, and marketing. The medical device development cycle, on a broad scope, is not well defined. The lack of a universal language with which to describe this process has made it difficult to understand and communicate. In this project, data was collected from peer-reviewed sources, summarized in a literature review, drawn out by hand into a series of lower-level process maps and finally assembled into a single process map. This project is …

Energy Harvesting From Exercise Machines Cal Poly Recreation Center Implementation, Justin Arakaki, Praveen Lawrence, Audrey Nakamura

Electrical Engineering

As Cal Poly moves towards an environmentally friendly campus, the Energy Harvesting from Exercise Machines (EHFEM) project looks to generate a new source of renewable energy to help power the Cal Poly Recreation Center. The project focuses on retrofitting exercise machines to generate electricity. As of December 2009, Audrey Nakamura, Justin Arakaki, and Praveen Lawrence developed a design which allows the contribution of electricity generated from multiple exercise machines into the power grid.

The project will require an investigation of retrofitted machine designs by Cal Poly students and commercially available products and systems. The information obtained from the product research …

An Assessment Of Linear Wrist Motion During The Taking Of A Bite Of Food, Meredith Drennan

All Theses

Motivated by the goal of building a device capable of detecting when a user takes a bite of food, we examine whether there is a correlation between linear wrist motion and bite detection. Such a device could be used to manage weight loss by tracking eating trends over time and providing real time feedback to the user regarding eating rate or total consumption. A comprehensive analysis of tracking linear motion using low cost inertial sensors is presented. Based on this theory, we discuss the design and tracking accuracy of a prototype bite counting device. We show that tracking position using …

Economics Of Using Biomass Electric Power For Energy Application Of Light Commercial Building, Madan M. Nellore

Masters Theses and Doctoral Dissertations

Due to increased concern on global warming coupled with dwindling supply of conventional fuels, attention has been focus ed recently on use of renewable energy resources. The energy derived from biomass has a great potential in meeting this challenge by use of the state of art technology in gasifiers. Commercial units recently placed in the market have a potential of using the energy available in the biomass in producing electric power as well as in meeting the other building loads most efficiently, with negligible environmental effect. Woody mass, switch grass, hazzle nut shells and other biomass wastes can be utilized …

Characterization And Physicochemical Modifications Of Polymer Hollow Fiber Membranes For Biomedical And Bioprocessing Applications, Benjamin R. Madsen

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Hollow fiber membranes (HFMs) formed through phase inversion methods exhibit specific physicochemical characteristics and generally favorable surface and mechanical properties, supporting their use in diverse applications including ultrafiltration, dialysis, cell culture, bioreactors, and tissue engineering. Characterization of, and modifications to, such membranes are important steps in achieving desired characteristics for specific applications.

HFMs subject to gas, irradiation, and chemical sterilization techniques were characterized based on several analytical techniques. It was revealed that these common sterilization techniques can cause inadvertent changes to HFM properties. While these changes may cause detrimental effects to HFMs used in filtration, the methods of sterilization are …

Transesterification And Recovery Of Intracellular Lipids Using A Single Step Reactive Extraction, Daniel R. Nelson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

A single-step, extractive reaction for extraction of lipids such as biodiesel components, omega-3 fatty acids, or other triglycerides from microbial cells was examined. Conventional methods for lipid extraction use toxic solvents, and require multiple steps and long processing times. When the goal is to produce fatty acid methyl esters or FAMEs, the extracted lipids are subjected to a separate transesterification reaction with simple alcohols in the presence of an acid or base catalyst. A simplified, single-step reactive extraction method can be applied that combines the sequential extraction followed by transesterification using acidified alcohols - a process known as in situ …

The Hypericum Perforatum Herb As An Antimycobacterial Agent And Its Implications As An Additional Tuberculosis Medication, Trent W. Mortensen

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

An immediate demand exists for new tuberculosis (TB) antibiotics due to the ever-increasing spread of drug-resistant strains. The drug-development process goes through four phases, the first (Phase 0) of which is to demonstrate and investigate drug effectiveness and toxicity. This research investigated the effectiveness of the Hypericum perforatum herb (commonly St. John's wort (SJW)) in its growth inhibition of mycobacteria and its viability effect on human lung cells.

Organic-solvent SJW extracts were effective at inhibiting every nonpathogenic genetically sequenced Mycobacterium isolate currently available (six isolates) in preliminary studies. Quantitative studies of five Mycobacterium isolates showed an order of concentration sensitivity …

The Use Of Factor Analysis In The Development Of Hand Sizes For Glove Design, Trevor M. Mclain

Department of Industrial and Management Systems Engineering: Dissertations, Theses, and Student Research

Factor analysis was used to develop a more detailed description of the human hand to be used in the creation of glove sizes; currently gloves sizes are small, medium, and large. The created glove sizes provide glove designers with the ability to create a glove design that can provide fit to the majority of hand variations in both the male and female populations. The research used the American National Survey (ANSUR) data that was collected in 1988. This data contains eighty-six length, width, height, and circumference measurements of the human hand for one thousand male subjects and thirteen hundred female …

2010 - The Fifteenth Annual Symposium Of Student Scholars

Symposium of Student Scholars Program Books

The full program book from the Fifteenth Annual Symposium of Student Scholars, held on April 27, 2010. Includes abstracts from the presentations and posters.

Women In The Aviation Industry, Maureen N. Mutisya

Senior Honors Theses

The Aviation industry has developed extensively since its establishment by the Wright Brothers in 1903. Over time, the highly male-dominated industry experienced significant changes to incorporate female aviators. Determined women initiated this process through participating in the aircraft engineering sector and pursuing careers as pilots. However, these women faced various challenges, which resulted in setbacks to their effective growth in the industry. In fact, vital issues encountered in the past are still present and often overlooked in today’s aviation industry. Therefore, identifying these problems and proposing solutions with effective corrective measures is necessary to increase and motivate female pilots globally.

Estimation Of Manning Roughness Coefficients On Precast Ecological Concrete Blocks, Te-Hsing Chang, Shen-Ting Huang, Shinne Chen, Jun-Cheng Lai

Journal of Marine Science and Technology–Taiwan

Agricultural irrigation and drainage canals in Taiwan are generally constructed using concrete. Concrete is used for the safety of conveyance efficiency. It also reduced loss and is structurally safe. The irrigation ditches and soil revetments in Taiwan fitted with a strong concrete lining. The smooth nonporous concrete surface makes the biology perch difficult and causes a monotonous flow. For these reason, an artificial materials-precast ecological concrete blocks (PECBs) was developed in this research. This was done in order to modify both the traditional concrete lining and the natural field materials in canal construction. Precast ecological concrete blocks (PECBs) were tested …

Sustainable Portland: Implementation Series 3, New England Environmental Finance Center

Climate Change

This report is the third in a series of efforts by students at the Muskie School of Public Service, Community Planning and Development Master’s program, in a core class called “Sustainable Communities.” In this course students seek to understand principles of sustainability and how efforts to implement Sustainability programs can become more successful. The report assembles term papers students completed on particular efforts by municipalities, universities, and other groups to achieve sustainability goals. Students worked on each project in a service learning format with real world clients. They were asked to fashion their papers around lessons learned by other organizations …

Impulse, Dana Hess, Kyle Johnson, Eric Landwehr, Cindy Rickeman

Impulse (Jerome J. Lohr College of Engineering Publication)

College:
[Page] 2 The Hanson siblings: Karin and Erik Hanson, of Sioux Falls, find themselves in the spotlight outside their engineering environment and the focus of younger engineering students within the engineering community.
[Page] 4 Reviving a tradition: Engineering students are helping to make the Hobo Day parade the visual attraction it once was through a competitive float-building effort.
[Page] 6 Softball & engineering: Sisters Brittany, Brooke, and Brianna Postma are pretty good at figuring percentages, whether that be in the classroom or on the field.
[Page] 8 Engineering Expo leadership: ‘The Scrambler’ event helps launch Darin and Derek Waldner …

Petroleum Degradation In Soil By Thermophilic Bacteria With Biopile Reactor, Astri Nugroho, Edison Effendi, Yulius Karonta

Makara Journal of Technology

Crude oil degradation has been carried out using biopile reactor in total petroleum hydrocarbon (TPH) concentration of 5%, 10% and 15%. The thermophilic microorganisms used from isolation result and identification are Aeromonas salmonicida, Bacillus pantothenticus, and Stenotrophomonas maltophilia. The biodegradation of biopile reactor is done by various concentrations of TPH, total plate count (TPC), and volatile suspended solid (VSS) per day for 30 days. The biodegradation of kinetic parameter calculated consists of μ, μm, Y, Yt, Yobs, Kd, Ks from TPH concentration decision, and TPC and VSS in every microorganism with t (observation time) of 0 hour to 168 hours. …

Poster Session, Kentucky Water Resources Research Institute, University Of Kentucky

Kentucky Water Resources Annual Symposium

• River Basins of Kentucky, Dan Carey, Kentucky Geological Survey
• An Initial Prioritization Approach for Potential Agricultural Best Management Practice Implementation Based on Subwatershed Indicators and Expert Knowledge, Brian Lee and Corey Wilson, Dept Landscape Architecture, UK
• Locating Karst Conduits in Cane Run Watershed of Central Kentucky Using Electrical Resistivity Methods, Junfeng Zhu and others, Kentucky Geological Survey
• Identification of DNA Biomarkers for Determining Sources of Fecal Pollution in Water, Rick Fowler and others, WATERS Laboratory, WKU
• Integrating Participatory Communication and Structured Public Involvement Processes to Better Address Superfund Issues: The Paducah Gaseous Diffusion Plant Future State …

Optimization Of Environmental Conditions To Maximize Carbon Dioxide Sequestration Through Algal Growth, Kenneth M. Karcher

Theses and Dissertations

The micro-alga Chlorella vulgaris was cultivated under a variety of environmental conditions in various culture media solutions to assess and optimize growth rate and biomass productivity. Efforts during this work investigated growth parameters at the micro-scale in an air-lift bubble system with the goal of interpreting performance characteristics that can be applied to a larger serpentine tubular Photo-bioreactor. Maximum growth rates and biomass yields were 0.65 d^-1 and 2.003 g biomass/L and achieved in seven days using urea in de-ionized water under a 24:0 Photoperiod (Light:Dark). Additionally, growth rates and biomass yields of 0.65 d^-1 and 1.964 g …

Nitrogen And Phosphorus Biomass-Kinetic Model For Chlorella Vulgaris In A Biofuel Production Scheme, William M. Rowley

Theses and Dissertations

Chlorella vulgaris was cultured in microbioreactors using Bold’s Basal medium at varying nitrogen and phosphorus concentrations to define nitrogen and phosphorus utilization standards. Nutrient concentration was varied between 137 mg/L to 7.33 mg/L NO3-N and between 55.2 mg/L to11.0 mg/L PO4-P in five test scenarios. All were grown under a constant photoperiod at 22±2 °C and a mixture of 4 to 10% carbon dioxide/air. Maximum yield and growth rate occurred with the highest initial nitrogen and phosphorus concentrations. A statistically significant difference in biomass was found among all test levels at the end of the eight day growth period. Applying …

Mercury In The Environment: Field Studies From Tampa, Bolivia, And Guyana, Joniqua A'Ja Howard

USF Tampa Graduate Theses and Dissertations

Tampa (US), Guyana (SA), and Bolivia (SA), are geographically, socially, economically, and politically unique which make them ideal sites to study issues of mercury and sustainability. Mercury’s innate ability to bioaccumulate and biomagnify in aquatic and terrestrial ecosystems poses a severe threat to both human and environmental health. The most vulnerable populations affected by mercury consumption include coastal communities, children, women of child-bearing age, the indigenous poor and persons with high environmental/occupational exposure factors. Communities in the regions of Florida, Bolivia, and Guyana whose diets are high in fish and are environmentally/occupationally exposed to mercury may be at a higher …