Digital Commons Network^™

Development Of A Cultivation System For Producing Pure Mycelium Materials From Distillers' Spent Grains, Keya Rani Roy

Theses and Dissertations--Biosystems and Agricultural Engineering

Stillage is a byproduct of distilleries which is rich in organic matter, minerals, and acidic components. It is commonly used as animal feed and has high potential for use as an alternative substrate for microorganisms. Filamentous fungi are saprophytes that can utilize stillage solids to grow as threadlike mycelium. The structure and composition of the filamentous mycelium has shown promise to produce pure mycelium materials (PMM), which might have many potential applications, for instance as a leather-like material or alternative protein food ingredient. Basidiomycota fungi (including true mushrooms) species are presently used in industry to produce PMM due to the …

Development Of Non-Destructive Methods For The Detection And Quantification Of Gluten Sources In Foods, Adewale Abiola Oloyede

Theses and Dissertations--Biosystems and Agricultural Engineering

Cross-contamination of food with gluten-rich grains such as wheat, barley, and rye remains a significant challenge in gluten-free food production, posing health risks to individuals with gluten-related disorders. Traditional detection methods are often time-consuming and labor-intensive, highlighting the need for rapid and reliable alternatives. This study investigates the use of hyperspectral imaging (HSI) combined with machine learning (ML) and deep learning (DL) techniques for the detection and quantification of gluten contamination in grain-based foods through three main approaches: (i) classification of gluten source contaminants in gluten-free flour, (ii) quantification of gluten content from wheat, rye, and barley flours, and …

Ahp–Python Framework For Multicriteria Modeling Of Rice Production In Asean, Mayang Anglingsari Putri, Risqy Siwi Pradini, Anuraga Jayanegara, Alexander Dimas Yonanta Putra

Knowledge Engineering and Data Science

Rice production is a key indicator of food security and agricultural stability in Southeast Asia, especially among Association of Southeast Asian Nations (ASEAN) countries. Despite shared regional goals, disparities in rice production remain, and previous studies mainly rely on descriptive statistics, lacking structured multicriteria decision-making frameworks and computational tools for cross-country comparisons. This study addresses these gaps by proposing an integrated Analytic Hierarchy Process (AHP)–Python framework to evaluate and rank ASEAN rice production from 2013 to 2022. Three criteria are used: Total Production Volume (K1), Production Growth Trend (K2), and Recent Year Performance (K3), capturing both long-term consistency and short-term …

Microbial Inactivation And Spray Drying For The Recovery Of Single-Cell Protein From Chemically Deconstructed Polyethylene Terephthalate, Reeba Elizabeth Reji

Dissertations, Master's Theses and Master's Reports

Polyethylene terephthalate (PET) is one of the most widely used polymers in plastics packaging. However, the increasing demand for PET has created challenges in waste disposal. Previous research has shown that PET can be deconstructed using ammonium hydroxide (CDPET). Microorganisms capable of utilizing the product as a sole carbon source can be converted to single-cell protein (SCP). A custom microbial inactivation unit was developed to ensure the safety and stability of generated SCP. Our study evaluated the effect of exposure time (45–60 minutes) at 140 °C with the goal of maximizing microbial inactivation. Our findings suggest that microbial inactivation can …

Community Diversity And Makeup Affect The Capacity For Bioconversion Of Chemically Deconstructed Pet Plastic Waste Into Biomass, Hunter Stoddard

Dissertations, Master's Theses and Master's Reports

Plastic waste management is an issue worldwide, particularly for locations with challenging logistics, such as remote locations, those impacted by natural disasters, and regions with armed conflict. These locations also struggle with food logistics and malnutrition. It is possible to tackle both issues by combining chemical deconstruction to depolymerize plastic waste with microbial conversion of the liquid product into biomass that can be used as an edible protein powder. The focus of this work is on the bioconversion of products from the chemical deconstruction of polyethylene terephthalate (PET) using ammonium hydroxide. Microbial communities have previously been shown to utilize the …

Analysis Of Crowd Logistics Networks Using Agent-Based Models, Preetam Kulkarni

Industrial, Manufacturing, and Systems Engineering Dissertations - Archive

Crowd logistics is a system in which an online platform connects a group of non-professional couriers (crowd/carriers), who use their under-utilized resources to offer delivery service to other individuals or businesses (senders) for a fee. While crowd logistics platforms have the potential to offer more flexible and responsive delivery services for much lower rates than traditional logistics providers, it is difficult for platforms to be successful as it is challenging to meet carriers’ and senders’ expectations. Crowd logistics has been applied in the context of food and grocery delivery, parcel pickup and drop-off services and last-mile delivery, however, it has …

Gold Nanoparticle-Enhanced Molecularly Imprinted Polymer Electrode For Non-Enzymatic Lactate Sensing, Christopher Animashaun, Abdellatif Ait Lahcen, Gymama Slaughter

Center for Bioelectronics Publications

We are reporting the development of a high-performance, non-enzymatic electrochemical biosensor for selective lactate detection, integrating laser-induced graphene (LIG), gold nanoparticles (AuNPs), and a molecularly imprinted polymer (MIP) synthesized from poly(3,4-ethylenedioxythiophene) (PEDOT). The LIG electrode offers a highly porous, conductive scaffold, while electrodeposited AuNPs enhance catalytic activity and signal amplification. The PEDOT-based MIP layer, electropolymerized via cyclic voltammetry, imparts molecular specificity by creating lactate-specific binding sites. Cyclic voltammetry confirmed successful molecular imprinting and enhanced interfacial electron transfer. The resulting LIG/AuNPs/MIP biosensor demonstrated a wide linear detection range from 0.1 µM to 2500 µM, with a sensitivity of 22.42 µA/log(µM) and …

Evaluation Of Heavy Metal Bioaccumulation And Growth In Hemp From Chicken Manure, Vermicompost, And Wastewater Sludge With Biochar And Health Risk Assessment, Chutiphan Sangsoda

Chulalongkorn University Theses and Dissertations (Chula ETD)

Hemp (Cannabis sativa L.) has become an economically valuable crop with a wide range of benefits. To promote more sustainable agricultural systems, the use of recycled substrates has increasingly replaced traditional fertilizers. However, substrates such as dry chicken manure, vermicompost, and wastewater sludge (WWS) combined with biochar often contain heavy metal contaminants, which may pose health risks through exposure to hemp. This study investigated three cultivars Rosella, Superwoman S1, and Red Robin over a 120-day period. The objectives were: (1) to compare the effects of dry chicken manure, vermicompost, and wastewater sludge (with and without biochar) on soil nutrient quality …

The Genetic Role Of The Dopamine Receptor D4 Gene, Zayden Hill

Student Research Design & Innovation Symposium

Attention deficit hyperactivity disorder (ADHD) is a neurodevelopmental disorder that affects over 22 million Americans today. Typically, ADHD is first presented during childhood, the exact cause of ADHD is unknown. Genetic mutations, such as those in the DRD4 gene on chromosome 11, have been linked to ADHD. This gene encodes for dopamine receptor D4, which inhibits adenylyl cyclase, an enzyme involved in cell signaling. A missense mutation in DRD4 could hinder the receptor function, contributing to inattentiveness, hyperactivity and impulsivity--core symptoms of ADHD. Epigenetics also plays a role in ADHD development and symptomology. Maternal omega-3 supplementation has been linked to …

Harlem Baking Co., Carm Reed, Julia Tyras, Matteo Lopergolo

Student Research Design & Innovation Symposium

Harlem Baking Co. (HBC) is a business located in New York City that handmakes "Little Jars of Happiness." Their desserts come in a variety of flavors (from New York cheesecake to chocolate lava cake) and are baked inside reusable Jars. The team was tasked with redesigning their logo and packaging in partnership with Dr. Jeff Binczyk's marketing class, and designed a website, fridge wrap, and AD campaign. HBC was also kind enough to send samples of their desserts for a photoshoot, which resulted in some amazing photos that are currently on display in their current ads.

Leaf-Based Varietal Categorization Of Sweetpotato (Ipomoea Batatas L. Lam.), A Potentially Healthful Vegetable, Using Image Processing And K-Means Clustering, Shahidul Islam, Md Towfiqur Rahman, Md Hamidul Rahman, Abdul Momin

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

Sweetpotato (Ipomoea batatas Lam) leaves contain higher concentrations of phenolic compounds, flavonoids, and carotenoids that are remarkable in health promotion. However, the nutrient content in sweetpotato leaves varies from variety to variety, and leaf shape and color are the key identifying factors for the varietal classification of sweetpotatoes. So, detecting sweetpotato leaves is essential for the in-situ identification of sweetpotato varieties and for developing intelligent agricultural systems. This study aimed to create a leaf-shape-based varietal classification technique for sweetpotato using image processing techniques coupled with a K-means clustering algorithm. 38 leaf images (RGB) of two sweetpotato cultivars were collected …

Proceedings Of The Tenth Annual Indiana Stem Education Conference: Generational Advances Through Stem Education, William S. Walker Iii, Lynn A. Bryan, S Selcen Guzey

Indiana STEM Education Conference

The Proceedings of the Tenth Annual Indiana STEM Education Conference are edited by the Center for Advancing the Teaching and Learning of STEM (CATALYST, https://www.education.purdue.edu/catalyst/) at Purdue University. The theme for the 2025 conference is Generational Advances through STEM Education. This year’s conference provides opportunities to learn about effective STEM education strategies, curriculum, and resources to engage students by considering the advances and impact of STEM.

Towards A Framework To Integrate Smart Urban Agriculture Into City Planning In Egypt, Heba Adel Ahmed Hussein

Mansoura Engineering Journal

This study discusses challenges facing the expansion of urban agriculture in Egypt, investigates overcoming these challenges using smart solutions, and proposes a framework to integrate smart urban agriculture (SUA) into Egyptian cities’ plans. The proposed framework provides an insight into the aspects that should be considered before, during, and after transformation to SUA. This study could serve as a basis for the integration of smart agriculture into urban planning theories and practices, and as an inspiration for urban planners, designers, landscape architects, policymakers, and decision-makers to create strategies for promoting digital transformation in Egyptian cities.

Arginine-Modified Surface Coffee Grounds Activated Carbon For Pb2+ Adsorption: Kinetic, Isotherm And Thermodynamic Studies, Jarawee Chupirom, Sirikorn Khaophong, Nopporn Hemyakorn, Sonchai Intachai, Panita Kongsune

Journal of Metals, Materials and Minerals

This research focuses on enhancing the value of coffee waste by converting it into activated carbon for removing Pb²⁺ ions from contaminated water. Coffee grounds were activated with KOH and carbonized at 600°C for 4 h to produce activated carbon (CGAC), then modified with arginine via hydrothermal treatment at 120°C for 48 h to create Arg-CGAC. The adsorbents' chemical and physical properties were analyzed using SEM, FT-IR, BET, and pHpzc methods. Batch experiments revealed that Arg-CGAC exhibited superior Pb2+ adsorption (223.10 mg∙g‒1) compared to CGAC (119.23 mg∙g‒1) under optimal conditions. Isotherm analysis showed stronger adsorbate-adsorbent interactions and a more endothermic …

Synergistic Effects Of Co-Ni Nanoalloys On Sio2@Tio2: A Novel Approach To Photocatalytic Tetracycline Degradation, Pratyush Kumar Sahu, Alaka Rath, Aslisha Champati, Ashish Madual, Pravat Manjari Mishra, Abanti Pradhan, Brundabana Naik

Journal of Metals, Materials and Minerals

To develop a cost-effective alternative to noble metal catalysts for photocatalysis, we synthesized a series of bimetallic Co-Ni nanoalloys on core-shelled SiO2@TiO2 nanostructures via a chemical reduction approach. The research demonstrates the photocatalytic behaviour of the Co-Ni alloy-enhanced SiO2@TiO2 (ST-Co, Ni) hybrid nanocatalyst, focusing on its crystalline structures, morphological shapes, chemical conditions, and optical properties, utilizing techniques such as X-ray diffraction, field emission scanning electron microscopy, UV-visible diffuse reflectance spectroscopy, photoluminescence spectroscopy, transmission electron microscope, and electrochemical analysis. The catalyst was evaluated for its effectiveness in degrading tetracycline, an overused antibiotic that persists in aquatic environments and is known for …

Enhancing Efficiency And Productivity Of Microbial Fuel Cells For Optimization Of Wastewater Treatment With Bioenergy Production, Diptimayee Pradhan, Krishna Pada Bauri, Debarpita Ghosal, Satchidananda Mishra, Abhijeet Das, Aditya Raj Singh, Akhil Gedela, Kumbhakarna Mallik, Mohammed Hassan Ali Hbag

Journal of Metals, Materials and Minerals

The primary objective is to remove the pollutants from the wastewater streams and reintegrate them into the water cycle, therefore safeguarding the environment and promoting public health. The utilization of membrane fuel cells (MFCs) enables the conversion of organic molecules present in wastewater into electrical energy, while concurrently generating potable water. To optimize the process, it is essential to understand the parameters that affect the performance and the relevant techniques such as electrode materials, microbial collectives, and operating settings to improve the efficiency and performance of MFC. Thus, it aims to present novel ideas that support the real-world deployment of …

Preventive Health Care Information Seeking Behaviors Among Baby Boomers In Taiwan, Alexander N. Chen, Michael J. Rubach, Tracy Suter, Hsin Ke Lu, Mark E. Mcmurtrey

Journal of International Technology and Information Management

Preventive health care is widely acknowledged as one of the most effective ways to reduce medical costs and enhance people's health. Preventive health care information (PHCI) is a crucial component. This study examines the PHCI-seeking behaviors of Taiwanese baby boomers. The study found some support for the idea that the preferred media used influenced the likelihood of Information seeking behavior. People with good health conditions were found to be more likely to seek PHCI, while people with greater health care needs sought out PHCI less frequently. The study examined social influences, which were found to be important. Three different types …

Characterization Of Multispecies Microbial Communities At Beef And Pork Processing Plants And Their Impact On Pathogen Stress Tolerance, Rong Wang, Sapna Chitlapilly Dass, Vignesh Palanisamy, You Zhou, Tatum Katz, Joseph M. Bosilevac

Nebraska Center for Biotechnology: Faculty and Staff Publications

Escherichia coli O157:H7 and Salmonella enterica are major foodborne pathogens. Biofilm formation may potentially contribute to product contamination by these pathogens at meat processing facilities. Further, pathogen stress tolerance may vary significantly due to the interactions with the multispecies microbial community at meat plants, which may be affected by processing activity, animal species, and the local selective pressure caused by sanitization practices.

We characterized natural microorganisms collected from floor drains at various areas at three beef plants and two pork plants and analyzed their impact on pathogen sanitizer tolerance.

The pathogen strains were able to integrate efficiently into the multispecies …

Diel Variations In Abundance Of Viral, Picoplankton, And Nanoplankton In Coastal Waters Of Taiwan In Spring, Patrichka Wei-Yi Chen, Madeline Olivia, Vladimir Mukhanov, Ufimtseva Margarita, Liashko Tatiana, Sakhon Evgenii, Supriyadi Supriyadi, Intan Dwi Puspitasari, Arpita Ghosh, An-Yi Tsai

Journal of Marine Science and Technology–Taiwan

The diel variations of viruses (< 0.2 μm), picoplankton (0.2-2 μm; heterotrophic bacteria, Synechococcus spp., and picoeukaryotes), and nanoflagellates (2-20 μm; heterotrophic and pigmented nanoflagellates) were examined for 2 days with approximately 4 h of temporal sampling in the spring of 2024 in coastal surface waters of the subtropical western Pacific. The abundance of bacteria did not follow a consistent diel pattern, and viral abundance was positively correlated with bacterial abundance. Synechococcus spp. was found to grow during the light period and with peak abundance at night, exhibiting a remarkably diel pattern; however, the pattern was opposite in picoeukaryotes. In the size-fractionation experiments, after 32 h of …

Wearable Disposable Elecetrotherapy, Mohamad Fallahrad

Dissertations and Theses

Non-invasive electrotherapies offer promising treatments for a wide range of conditions such as pain, headaches, depression, addiction, cognitive decline, wound healing, and drug delivery. However, patient compliance remains low due to the high initial cost, cumbersomeness and inconvenience of existing technology compared to pharmaceuticals. Current electrotherapy devices are often bulky and require users to connect disposable electrodes, initiate programs, and manage charging cycles, which hinders widespread adoption.

We address these challenges by designing and validating a novel electrotherapy platform that is single-use, disposable, low-cost, and wearable. Activated automatically upon skin contact, the device delivers a preset dose of controlled electrical …

Food Rescue, Food Waste, And Policy In New York: A Mixed-Methods Study Of Food System Impacts, Mariana Torres Arroyo

Electronic Theses & Dissertations (2024 - present)

This dissertation investigates how food donation policies in New York State influence the recovery, redistribution, and waste of surplus fresh produce, with a focus on fruits and vegetables. Using an interdisciplinary, systems-based approach that integrates system dynamics modeling with qualitative, community-engaged research, the study analyzes the effectiveness and unintended consequences of three major policies: Nourish New York, the Farm to Food Bank tax credit, and the Food Donation and Food Scraps Recycling Law.

Chapter 2 uses simulation modeling to examine how the tax credit and waste ban affect produce recovery and redistribution. Results show that while both policies can boost …

Develop A Cross-Platform Toolset For In Situ Deep-Sea Coral Propagation, Joe Bevilacqua

Open Access Master's Theses

Mesophotic and deep-sea gorgonian octocorals in the Gulf of Mexico were significantly damaged following the Deepwater Horizon oil spill, prompting a major federal investment in developing restoration techniques for these fragile, slow-growing communities. Asexual, in-situ coral propagation offers a biologically viable path for restoration at depth. However, current ROV systems are not optimized for the delicate, fine-scale interactions required to perform this process reliably. The objective of this research was to design, prototype, and evaluate a ROV-mountable toolset capable of preparing octocoral fragments for potting in epoxy substrate, with the additional goal of integrating the toolset into URI’s Coral Carousel …

Molecular Characterization Of Leptin Gene In Sheep (Ovis Aries), Shahenda M. Hegazy, Mohamed Fouda, Ahmed Ateya, Huda A. El Emam

Mansoura Veterinary Medical Journal

INTRODUCTION: Advances in molecular genetic techniques have simplified the process of identifying individual differences at the DNA level. Genetic polymorphisms at candidate genes controlling economic features have recently drawn a lot of scholarly attention due to their potential as a tool for genetic selection and the identification of evolutionary linkages in livestock. OBJECTIVE: This work aimed to detect nucleotide sequence variants in Lep gene as genetic markers for growth and heat tolerance traits in Barki and Abudeleik sheep using PCR-DNA sequencing approach in search of single nucleotide polymorphisms (SNPs). METHODS: 30 female ewes, 15 of each breed (Barki and Aboudeleik) …

Development Of Food Preservative Hydrogel Using Bioactive Compounds Extracted From Canola Meal, Kayla Christopherson

Electronic Theses and Dissertations

With an ever-growing population comes the need for a greater food supply. Yet land and other agricultural resources put a limit on the availability of food production. As farmers strive to optimize higher produce yields, society must turn to greener, more sustainable food preservation techniques to reduce food spoilage. The inclusion of extracted bioactive compounds found in canola meal into a food preservative hydrogel is such a solution for food spoilage reduction. In this thesis, extraction parameters of glucosinolates were examined along with their inhibition of E. coli DH5αZ1 when incorporated into 2 developed hydrogels: gelatin A Schiff-base and agar-agar. …

Intercellular Mitochondrial Transfer Contributes To Microenvironmental Redirection Of Cancer Cell Fate, Julie Sofie Bjerring, Yara Khodour, Emilee Anne Peterson, Patrick Christian Sachs, Robert David Bruno

School of Medical Diagnostics & Translational Sciences Publications

The mammary microenvironment has been shown to suppress tumor progression by redirecting cancer cells to adopt a normal mammary epithelial progenitor fate in vivo. However, the mechanism(s) by which this alteration occurs has yet to be defined. Here, we test the hypothesis that mitochondrial transfer from normal mammary epithelial cells to breast cancer cells plays a role in this redirection process. We evaluate mitochondrial transfer in 2D and 3D organoids using our unique 3D bioprinting system to produce chimeric organoids containing normal and cancer cells. We demonstrate that breast cancer tumoroid growth is hindered following interaction with mammary epithelial cells …

Using A Water Quality Index To Assess The Impacts Of Heavy Metals Contamination For Sustainable Water Management: A Case Study Of The Bang Pakong River, Thailand, Khin Yadana Soe, Pokchat Chutivisut, Wilailuk Niyommaneerat

Applied Environmental Research

Anthropogenic activities such as improper waste disposal, excessive fertilizer use, and industrial effluents cause heavy metal contamination in water, soil, and the atmosphere. Herein, the water quality index (WQI) was used to characterize heavy metal (i.e., cadmium (Cd), arsenic (As), and mercury (Hg)) contamination in the Bang Pakong River, Thailand. Specifically, water samples were collected during the dry season from four stations along the river from 2012–2022. The heavy metal concentrations in the samples were analyzed via atomic absorption spectrophotometry, and WQI values were calculated on the basis of standard water quality parameters. At the four sampling stations, high As …

Halotolerant Biodegradation And Detoxification Of Synthetic Dyes By Marine-Derived Penicillium Oxalicum M6a: Metabolite Profiling And Ecotoxicity Assessment, Ugochukwu Okechukwu Ozojiofor, Mohammed Sani Abdulsalami, Nkechi Eucharia Egbe, Ahmed Ali Haroun, Yakubu Magaji

Applied Environmental Research

The complex aromatic structures of synthetic dyes in contaminated water bodies make them difficult to degrade, posing a serious environmental challenge and representing a significant emerging pollutant that warrants urgent intervention. This study evaluated the biodegradation and detoxification potential of a halotolerant, marine-derived fungal strain, Penicillium oxalicum M6A, isolated from the Nigerian coastline, against Indigo carmine (IC) and Remazol Brilliant Blue R (RBBR). The effects of pH, temperature, salinity, and dye concentration on degradation efficiency, alongside enzymatic activity, were assessed. FTIR and GC‒MS analyses were employed to identify degradation products and predict metabolic pathways, whereas toxicity was evaluated in three …

Assessment Of Metal Contamination And Potential Health Risk Of Roadside Vegetables Along The Sayre Highway, Province Of Bukidnon, Philippines, John R.N. Labajo, Junesa U. Cristobal, Myrna G. Pabiona

Applied Environmental Research

This study assessed heavy metal contamination in roadside vegetables (Ipomoea batatas and Moringa oleifera) across five urbanized localities in Bukidnon, Philippines, and evaluated potential human health risks. Metal concentrations (Cd, Cr, Cu, Co, Fe, Mn, and Zn) were analyzed via atomic absorption spectro-photometry and compared against FAO/WHO maximum permissible limits. The results revealed severe contamination, with Mn (575.27–862.17 mg kg-1) and Fe (256.46–706.68 mg kg-1) dominating natural soil mineralogy (Adtuyon series) and anthropogenic inputs. Toxic Cr (16.20–21.93 mg kg-1) exceeded permissible limits by nine and a half times in Quezon. Health risk assessments revealed negligible noncarcinogenic risks (THQ …

Spatial Estimation Of Trophic State For Reservoir Using Ground Monitoring And Remote Sensing Data, Thong Nguyen Hoang, Van Tran Thi

Applied Environmental Research

Nutrient pollution, also known as eutrophication, is a severe environmental problem that leads to harmful algal blooms in water bodies and affects water supplies for human use. This study aims to determine the trophic state of the reservoir based on the trophic state index (TSI) calculated from ground monitoring data of Secchi depth (SD), total phosphorus (TP), and chlorophyll-a (Chl-a) obtained from 35 points in 2 survey periods during the late rainy season to early dry season 2012–2013 and May 2023. In addition, we combined remote sensing data to spatially estimate the eutrophication situation across the reservoir through correlation analysis …

Nationwide Household Food Waste Generation Rates In Rural And Urban Settings: Evidence In Thailand, Vilasinee Krutprong, Chanathip Pharino

Applied Environmental Research

To achieve sustainable development goals, addressing food waste requires evidence-based solutions grounded in a nuanced understanding of waste generation rates and the factors influencing them. This study explores the intricate dynamics of food waste generation in Thai households, encompassing both liquid and solid waste streams. Empirical data were collected from 2,700 diverse households across urban and rural areas over a four-week period. The study revealed an average food waste generation rate of 0.332±0.003 kg per capita per day when liquids were included and 0.228± 0.002 kg per capita per day when liquids were excluded. Surprisingly, rural households generated more food …