Digital Commons Network^™

Accumulation Of Lead By Biofilms In Drinking Water Distribution Systems, Kaleb Oren Belcher

Graduate Theses and Dissertations

Recent crises, such as the one in Flint, MI, indicate that lead exposure from drinking water is a major health concern in the United States. Over six million lead service lines are still in use in the United States, and a universal protocol to safely remove these lead service lines from drinking water distribution systems has not yet been established. This paper calls to attention the potential hazard that biofilms pose as a source of lead in distribution systems, even after the removal of lead pipes. This study used a simulated water distribution system containing a lead source pipe and …

Rapid Separation Of Bacteria From Blood For Sepsis Diagnosis, Mahsa Alizadeh

Theses and Dissertations

Sepsis is a severe blood infection caused by bacteria entering the blood stream. Sepsis caused by antibiotic resistant bacteria is very dangerous with a high mortality rate. The current clinical diagnostic methods for sepsis require culturing the blood sample prior to other steps of the diagnosis procedure. Culturing the blood samples is a time-consuming step which increases the time required for the diagnostic procedure. Considering the fact that the mortality rate of the sepsis increases as time passes, it is essential to find methods for sepsis diagnosis that do not require culturing the samples. The first step of a new …

Identification And Engineering Of Nonribosomal Peptide Biosynthetic Systems, Fuchao Xu

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This research focuses on the understanding and engineering of nonribosomal peptide biosynthetic pathways in Streptomyces coelicolor CH999, Escherichia coli BAP1 and Saccharomyces cerevisiae BJ5464-NpgA. The biosynthetic systems of indigoidine from bacteria and beauvericin/bassianolide from fungi were studied in this research. The production of these valuble products was significantly increased by enhancing their synthetic pathway with metabolic engineering approaches.

Indigoidine is a bacterial natural product with antioxidant and antimicrobial activities. Its bright blue color resembles the industrial dye indigo, thus representing a new natural blue dye that may find uses in industry. Indigo is a dark blue crystalline powder and has …

Studying The Use Of Microbial Induced Calcite Precipitation As A Shallow Stabilization Alternative To Treat Expansive Soils, Tasria Rahman

Boise State University Theses and Dissertations

Expansive soils usually recognized as swell-shrink soils have been a problem for civil infrastructure for a long time. It has been a very common practice to use chemical stabilizers including cement and lime to stabilize expansive soils, especially for lightly loaded structures. However, due to the the detrimental effects of these stabilizers on the environment and several occurrences of premature failures after stabilizing with chemical additives, engineers are in search of sustainable stabilization alternatives. Microbial Induced Calcite Precipitation (MICP) is a promising process, which can improve the properties of expansive soil through calcite precipitation. Previous research has shown promise for …

Nanoparticle Catalytic Enhancement Of Carbon Dioxide Reforming Of Methane For Hydrogen Production, Nicholas Groden

Doctoral Dissertations

The U.S. produces 5559.6 million metric tons of carbon dioxide annually, of which 21% is produced by industrial processes. Steam reforming, an industrial process that accounts for 95% of all hydrogen production in industry, produces 134.5 million metric tons of carbon dioxide or around 11% of the total carbon dioxide produced by industry. This carbon dioxide is then either emitted or goes through a sequestration process that accounts for 75% of the plant's operational costs. An alternative reaction to steam reforming is dry reforming, which utilizes carbon dioxide rather than emitting it and can be used in conjunction with current …

Smart Disease Prevention App: Informing The Public In Their Own Geographic Location, Apoorva Sulakhe, Shafali Rana, Zoe Disori, William Nogay, Kyle Plummer, Meredith Shannon, Morgan Young, Alyssa Zielinski, Vincent G. Duffy

Purdue Journal of Service-Learning and International Engagement

Apoorva Sulakhe and Shefali Rana are graduate students in the School of Industrial Engineering at Purdue. They have both been teaching assistants under their coauthor, Dr. Vincent Duffy, while supervising multiple projects. Coauthors Zoe Disori, William Nogay, Kyle Plummer, Meredith Shannon, Morgan Young, and Alyssa Zielinski are listed in alphabetical order. They were all seniors in School of Industrial Engineering at the time of this project in 2017. The purpose of their study, described in this article, was to develop an application to provide users with accurate information about diseases spreading in their geographic locations.

Comparison Of Bacterial And Viral Reduction Across Different Wastewater Treatment Processes, Aayushi R. Vagadia

USF Tampa Graduate Theses and Dissertations

Today billions of people live without access to basic sanitation facilities, and thousands die every week due to diseases caused by fecal contamination associated with improper sanitation. It has thus become crucial for decision makers to have access to relevant and sufficient data to implement appropriate solutions to these problems. The Global Water Pathogen Project http://www.waterpathogens.org/ is dedicated to providing an up-to-date source of data on pathogen reduction associated with different sanitation technologies that are important if the world is to achieve the Sustainable Development Goals (SDGs) related to health and sanitation provision. In this research, a subset of the …

Autonomous Ground Photographer, Sylvia Tan, Nicholas C. Smith, Kate Padilla, Elise Ashley

Mechanical Engineering Design Project Class

Dr. Penczykowski, a biology professor at Washington University in St. Louis, researches the effects of powdery mildew on three plant species. Her lab currently collects data in the field by manually counting the number of infected plants with the assistance of a simple PVC grid. Therefore, this projects aims to automate this process by capturing images that can then be processed in her lab after.

Governance For A Changing Climate: Adapting Boston’S Built Environment For Increased Flooding, Stephanie Kruel, Rebecca Herst, David Cash

Sustainable Solutions Lab

As the impacts of climate change intensify, coastal cities like Boston face increasing threats from sea level rise, extreme precipitation, and storm surges. This report, produced by the Sustainable Solutions Lab at the University of Massachusetts Boston, examines the role of governance in preparing Boston’s built environment for these evolving risks. Building upon the foundational work of the Climate Ready Boston initiative, the report outlines critical governance functions necessary for climate adaptation, including institutional coordination, regulatory reform, community engagement, and data-driven decision-making. Emphasizing a multiscalar governance approach, it analyzes the tools available at local, regional, state, and federal levels and …

Computational Thinking And Literacy, Sharin Rawhiya Jacob, Mark Warschauer

Journal of Computer Science Integration

Today’s students will enter a workforce that is powerfully shaped by computing. To be successful in a changing economy, students must learn to think algorithmically and computationally, to solve problems with varying levels of abstraction. These computational thinking skills have become so integrated into social function as to represent fundamental literacies. However, computer science has not been widely taught in K-12 schools. Efforts to create computer science standards and frameworks have yet to make their way into mandated course requirements. Despite a plethora of research on digital literacies, research on the role of computational thinking in the literature is sparse. …

Antimicrobial Efficacy And Safety Of A Novel Gas Plasma-Activated Catheter Lock Solution, Sudhir Bhatt, Poonam Mehta, Chen Chen, Dayle A. Daines, Leonard A. Mermel, Hai-Lan Chen, Michael G. Kong

Bioelectrics Publications

Antimicrobial lock solutions are important for prevention of microbial colonization and infection of long-term central venous catheters. We investigated the efficacy and safety of a novel antibiotic-free lock solution formed from gas plasma-activated disinfectant (PAD). Using a luminal biofilm model, viable cells of methicillin-resistant Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, and Candida albicans in mature biofilms were reduced by 6 to 8 orders of magnitude with a PAD lock for 60 min. Subsequent 24-h incubation of PAD-treated samples resulted in no detectable regrowth of viable bacteria or fungi. As a comparison, the use of a minocycline-EDTA-ethanol lock solution for 60 …

Evaluation Of Drug-Loaded Gold Nanoparticle Cytotoxicity As A Function Of Tumor Tissue Heterogeneity., Hunter Allan Miller

Electronic Theses and Dissertations

The inherent heterogeneity of tumor tissue presents a major challenge to nanoparticle-medicated drug delivery. This heterogeneity spans from the molecular to the cellular (cell types) and to the tissue (vasculature, extra-cellular matrix) scales. Here we employ computational modeling to evaluate therapeutic response as a function of vascular-induced tumor tissue heterogeneity. Using data with three-layered gold nanoparticles loaded with cisplatin, nanotherapy is simulated with different levels of tissue heterogeneity, and the treatment response is measured in terms of tumor regression. The results show that tumor vascular density non-trivially influences the nanoparticle uptake and washout, and the associated tissue response. The drug …

Studying The Applicability Of Biostimulated Calcite Precipitation In Stabilizing Expansive Soils, Md Touhidul Islam

Boise State University Theses and Dissertations

Of the four types of soils, clays are often associated with issues related to low bearing capacity, high compressibility, swelling and shrinking nature. For example, expansive soils swell and shrink with moisture ingress and digress and are prevalent in several parts of the world causing billions of dollars in damages annually to various civil infrastructures. Several ground improvement techniques such as chemical stabilization, deep soil mixing, moisture barriers, and others were employed to counteract these soils. However, these methods are impractical in certain situations and unsustainable in others due to their economic and environmental impacts. Microbiological treatment of soils could …

Whole Cell Cross-Linking To Discover Host-Microbe Protein Cognate Receptor/Ligand Pairs, Bart C. Weimer, Poyin Chen, Prerak T. Desai, Dong Chen, Jigna Shah

Biological Engineering Faculty Publications

Bacterial surface ligands mediate interactions with the host cell during association that determines the specific outcome for the host–microbe association. The association begins with receptors on the host cell binding ligands on the microbial cell to form a partnership that initiates responses in both cells. Methods to determine the specific cognate partnerships are lacking. Determining these molecular interactions between the host and microbial surfaces are difficult, yet crucial in defining biologically important events that are triggered during association of the microbiome, and critical in defining the initiating signal from the host membrane that results in pathology or commensal association. In …

In Vitro Cytocompatibility Of Antibacterial Levels Of Polymer Nitric Oxide Release, Rana Gbyli, Christina Zito, Kagya Amoako

Mechanical and Industrial Engineering Faculty Publications

Although the antibacterial property of nitric oxide (NO) has been well documented in gram positive and gram negative bacteria cultures, its cytotoxic effects are not completely clear. To limit potential in vivo cytotoxicity, our group recently investigated the effects of a range of NO fluxes on S. epidermidis and S. aureus to determine a minimum effective NO level. In this study, we report the antibacterial function of this minimum NO level also on gram negative Pseudomonas aeruginosa as well as its cytocompatibility effects on lung and kidney cells. Standardized bacterial cultures were treated with NO releasing PDMS substrates followed by …

Surgical And Medical Applications Of Drones: A Comprehensive Review, James C. Rosser, Vudatha Vignesh, Brent A. Terwilliger, Brett C. Parker

Publications

Background: Drones have the ability to gather real time data cost effectively, to deliver payloads and have initiated the rapid evolution of many industrial, commercial, and recreational applications. Unfortunately, there has been a slower expansion in the field of medicine. This article provides a comprehensive review of current and future drone applications in medicine, in hopes of empowering and inspiring more aggressive investigation. Database: A literature search was performed by EBSCO (Elton B. Stephens Company) Discovery Service, searching the phrases "drones," "UAV," "unmanned aerial vehicles," "UAS," and "unmanned aerial systems." A second search was used to identify sources that contained …

Surgical And Medical Applications Of Drones: A Comprehensive Review, Brent Terwilliger, James C. Rosser Jr., Vudatha Vignesh, Brett C. Parker

Publications

Drones have the ability to gather real time data cost effectively, to deliver payloads and have initiated the rapid evolution of many industrial, commercial, and recreational applications. Unfortunately, there has been a slower expansion in the field of medicine. This article provides a comprehensive review of current and future drone applications in medicine, in hopes of empowering and inspiring more aggressive investigation.

Antimicrobial Properties And Characterization Of Polymer/Composite Nanofibers, David De La Garza

Theses and Dissertations

Pathogenic bacteria have been the cause of many illnesses worldwide due to their rapid growth and reproduction in food and water. Bacteria-resistant antibiotics and kidney complications due to overuse of antibiotics has remained a large public health concern. The goal of this research project was to find an alternative by producing nanofibers that inhibit microbial growth utilizing Forcespinning technology. The Forcespinning of poly(acrylic acid) (PAA) with Single Wall Carbon Nanotubes functionalized with OH (SWCNT-OH) and Ag + flakes were carried out on solutions with different concentrations. Scanning electron microscopy was used to view fiber morphology and investigate the average nanofiber …

High-Titer Rapid Response Platform For Epidemic Preparedness, José Castillo

Vaccine Technology VII

Recent epidemics & pandemics have exposed serious flaws in global response to infectious disease outbreaks, as no vaccine were available to stop the diseases, due to limited market incentive.

New initiatives, such as the CEPI “Coalition for Epidemic Preparedness Innovations” are focusing on accelerating vaccine development for priority disease and ensuring their global availability.

In this light, Univercells is dedicated to designing novel biomanufacturing technologies to accommodate new products at short notice.

Univercells innovative manufacturing concept is designed to enable the rapid generation of potent vaccine candidates against a broad spectrum of targets, with the capacity to rapidly scale-up and …

Development Of A Vaccine Production Platform For Poultry Diseases In Africa: Newcastle Disease Virus Non-Replicative Adenovirus-Vectored Vaccine, Omar Farnos, Amine Kamen, Héla Kallel, Khaled Trabelsi, Martha Yami, Esayas Gelaye

Vaccine Technology VII

Poultry are a vital village livestock playing an important economic and nutritional role in the livelihoods of poor rural households in developing countries, including the Sub-Saharan Africa. Poultry production in Africa is threatened by infectious diseases such as Newcastle Disease (ND), which is highly contagious and endemic, with recurrent outbreaks that provoke heavy losses every year. ND is caused by the Newcastle disease virus (NDV), a negative-sense single-stranded RNA virus from the genus Avulavirus, family Paramyxoviridae. In particular, ND is one of the major problems in village chickens in most parts of Ethiopia where commercial poultry is routinely vaccinated …

Conference Program, Amine Kamen, Tarit Mukhopadhyay, Charles Lutsch, Nathalie Garçon

Vaccine Technology VII

No abstract provided.

Channel Capacity Analysis Of Diffusion-Based Molecular Communication Model, Cheng Zhen, Lin Fei, Yanjing Lei, Huiting Zhao

Journal of System Simulation

Abstract: At present, the research on the channel capacity of diffusion-based molecular communication (DBMC) model is very limited. In the slotted point to point communication scenario, considering all the inter-symbol interferences from all the previous time slots, the optimal decisive threshold is provided by using the criterion of minimum average error probability in the detection process for the receiver nanomachine, and the mutual information expression is derived in DBMC model. Thus channel capacity of this model is analyzed. The simulation results show that the different parameters have important influences on the channel capacity. Compared to the existed point to point …

Simulation Of Hot Event Propagation Based On Game Theory And Sirs, Dezhi Wei, Fuji Chen, Lina Lin

Journal of System Simulation

Abstract: The hot events of the internet public opinion spread fast and have great influence. It has become a topic of concern for netizens and governments. The spread of hot spots on the internet is similar to the dynamics of infectious diseases, so a traditional SIRS epidemic model based improved model is proposed, combining the analysis of the game theory. The evolutionary game model of infectious body and immune body is constructed, the equilibrium point of the game is found and the law of the spread of hot spots is obtained. The model is verified by experiment through the …

The Immunity Of The Many: Ethical Considerations Of Vaccinations In Light Of Herd Immunity, Sophia Koval

Dartmouth Undergraduate Journal of Science

No abstract provided.

Milkguard: Low-Cost, Polymer-Based Sensor For The Detection Of Escherichia Coli In Donated Human Breast Milk, Maggie May, Nicholas Kikuchi, Matthew Zweber

Bioengineering Senior Theses

Breast milk, the gold standard for infant nutrition, could prevent up to 13% of child deaths worldwide. However, many mothers are unable to breastfeed due to health conditions and other factors. Because of this, a network of more than 500+ human milk banks, which collect and distribute donated breast milk to infants, have emerged worldwide. However, operational costs to ensure the safety of this milk remain time-intensive and costly.

There are no existing diagnostics for rapid and on-site detection of bacterial contaminants in donated milk. Currently, many milk banks send samples to outside laboratories for bacterial culturing tests, which take …

Mechanism Of Virus Inactivation By Cold Atmospheric-Pressure Plasma And Plasma-Activated Water, Li Guo, Ruobing Xu, Lu Gou, Zhichao Liu, Yiming Zhao, Dingxin Liu, Lei Zhang, Hailan Chen, Michael G. Kong

Bioelectrics Publications

ABSTRACT Viruses cause serious pathogenic contamination that severely affects the environment and human health. Cold atmospheric-pressure plasma efficiently inactivates pathogenic bacteria; however, the mechanism of virus inactivation by plasma is not fully understood. In this study, surface plasma in argon mixed with 1% air and plasma-activated water was used to treat water containing bacteriophages. Both agents efficiently inactivated bacteriophages T4, ϕ174, and MS2 in a time-dependent manner. Prolonged storage had marginal effects on the antiviral activity of plasma-activated water. DNA and protein analysis revealed that the reactive species generated by plasma damaged both nucleic acids and proteins, consistent with the …

Bounded Rank Optimization For Effective And Efficient Emergency Response, Pallavi Madhusudan Manohar, Pradeep Varakantham, Hoong Chuin Lau

Research Collection School Of Computing and Information Systems

Effective placement of emergency response vehicles (such as ambulances, fire trucks, police cars) to deal with medical, fire or criminal activities can reduce the incident response time by few seconds, which in turn can potentially save a human life. Owing to its adoption in Emergency Medical Services (EMSs) worldwide, existing research on improving emergency response has focused on optimizing the objective of bounded time (i.e. number of incidents served in a fixed time). Due to the dependence of this objective on temporal uncertainty, optimizing the bounded time objective is challenging. In this paper, we propose a new objective referred to …

Carbon Sequestering Bio Concrete As A Solution To Growing Atmospheric Concentrations Of Co2, Connor Cech, Jackson Walker

Construction Management

High levels of anthropogenic carbon dioxide (CO₂) emissions into the atmosphere are propelling climate change, which has been proven to be highly detrimental to the planet. Cement production for the construction industry is a leading cause of these harmful carbon emissions. This project introduces a potential solution to this issue in the form of a new biological concrete that is treated with carbon sequestering bacteria of the Bacillus family to remove CO₂ from the atmosphere. The consolidated research presented in this project will explain the biology behind this process, and how it can be implemented in construction …

Quantifying Effects Of Neutrophil Memory On Migration Patterns Using Microfluidic Platforms And Ode Modeling Of The Mechanistic Molecular Pathways, Brittany P. Boribong, Mark J. Lenzi, Mirjam Sarah Kadelka, Stanca Ciupe, Liwu Li, Caroline N. Jonea

Biology and Medicine Through Mathematics Conference

No abstract provided.

Static And Dynamic Quantitative Microbial Risk Assessment Of Potable Reuse Paradigms, Erfaneh Amoueyan

UNLV Theses, Dissertations, Professional Papers, and Capstones

In recent years, potable reuse applications have become more common due to population growth and increased water demand, especially in communities with limited or variable water resources. However, there are concerns about potential exposure to pathogens and chemical compounds in treated wastewater. Therefore, advanced wastewater treatment processes are of paramount importance in any potable reuse system. The overall aim of this study was to develop and implement static and dynamic QMRAs to compare public health risk in various potable reuse scenarios. Cryptosporidium, norovirus, adenovirus, and Salmonella were chosen as the target pathogens. The research evaluated the performance of full advanced …