Digital Commons Network^™

Spectrophotometric Device For Early Malaria Detection, Adrian Wasylewski, Christopher Faber, Victor Lin

Honors Thesis

Malaria remains a significant global health challenge, with millions of cases reported annually, particularly in resource-limited regions. Timely and accurate diagnosis is crucial for effective treatment and disease management. Unfortunately, there are still significant challenges in existing testing procedures, which are often expensive, inaccurate, or impractical for mass testing. While spectrophotometry has been proposed as a detection tool for other tropical diseases such as dengue fever, no prototype has been made for any disease, including malaria. This report addresses this gap by presenting a spectrophotometric-based malaria detection tool that achieves high sensitivity at a low cost. The inclusion of a …

Towards A Study Protocol: A Data-Driven Workflow To Identify Error Sources In Direct Ink Write Mechatronics, Hein Htet Aung, Quynh D. Tran, Pawan K. Tripathi, Roger H. French, Laura S. Bruckman

Faculty Scholarship

Using Direct Ink Write (DIW) technology in a rapid and large-scale production requires reliable quality control for printed parts. Data streams generated during printing, such as print mechatronics, are massive and diverse which impedes extracting insights. In our study protocol approach, we developed a data-driven workflow to understand the behavior of sensor-measured X- and Y-axes positional errors with process parameters, such as print velocity and velocity control. We uncovered patterns showing that instantaneous changes in the velocity, when the build platform accelerates and decelerates, largely influence the positional errors, especially in the X-axis due to the hardware architecture. Since DIW …

Mathematical Modeling For Dental Decay Prevention In Children And Adolescents, Mahdiyeh Soltaninejad

Dissertations

The high prevalence of dental caries among children and adolescents, especially those from lower socio-economic backgrounds, is a significant nationwide health concern. Early prevention, such as dental sealants and fluoride varnish (FV), is essential, but access to this care remains limited and disparate. In this research, a national dataset is utilized to assess sealants' reach and effectiveness in preventing tooth decay, particularly focusing on 2nd molars that emerge during early adolescence, a current gap in the knowledge base. FV is recommended to be delivered during medical well-child visits to children who are not seeing a dentist. Challenges and facilitators in …

Nanozyme-Based Sensing Of Dopamine Using Cobalt-Doped Hydroxyapatite Nanocomposite From Waste Bones, Umar Nishan, Nighat Jabeen, Amir Badshah, Nawshad Muhammad, Mohibullah Shah, Irfan Ullah, Saifullah Afridi, Jibran Iqbal, Muhammad Asad, Riaz Ullah, Essam A. Ali, Sarfraz Ahmed, Suvash Chandra Ojha

All Works

Dopamine is one of the most important neurotransmitters and plays a crucial role in various neurological, renal, and cardiovascular systems. However, the abnormal levels of dopamine mainly point to Parkinson’s, Alzheimer’s, cardiovascular diseases, etc. Hydroxyapatite (HAp), owing to its catalytic nature, nanoporous structure, easy synthesis, and biocompatibility, is a promising matrix material. These characteristics make HAp a material of choice for doping metals such as cobalt. The synthesized cobalt-doped hydroxyapatite (Co-HAp) was used as a colorimetric sensing platform for dopamine. The successful synthesis of the platform was confirmed by characterization with FTIR, SEM, EDX, XRD, TGA, etc. The platform demonstrated …

Production Of Mrna Vaccine For Influenza, Christina Miller

Honors Theses

The objective of this project is to design a process to produce an mRNA influenza vaccine that serves as an alternative to conventional influenza vaccines, replacing current options with innovative mRNA technologies. Given that older populations are most likely to follow up on immunizations consistently, and the increasing average age worldwide, the market for this project appears to be expanding in the next 80 years. Additionally, experts predict more frequent mutations and outbreaks of viral disease from a variety of factors including globalization and global temperature trends. Rapid response to these viral outbreaks will continue to be as, or even …

Development Of Novel Analytical Methods For The Investigation Of Mammalian Tissues And Cells, Grace A. Binder

USF Tampa Graduate Theses and Dissertations

This dissertation endeavors to advance the frontiers of analytical chemistry by addressing critical challenges across three distinct chapters. In the first chapter, the focus is on the evaluation of digestion methods for the analysis of trace metals in mammalian tissues and the Standard Reference Material NIST 1577c. The primary goal is to optimize methodologies, laying the groundwork for reliable trace metal analysis and furthering our understanding of their roles in biological systems.

Transitioning into the realm of immunomodulation, the second chapter elucidates immune response, disease and treatment through the optimization and discovery of STING modulators, utilizing the luciferase assay for …

Formulation, Characterization, And In Vivo Immunogenicity Evaluation Of Heat-Stabilized Dissolvable Polymeric Microneedles, Aidan Lane Leyba

Biomedical Engineering ETDs

Since its introduction, vaccination has improved health outcomes tremendously. However, modern vaccination efforts are still difficult to implement, particularly in developing countries with warm climates. This includes complex and expensive cold-chain management of vaccines to maintain stability, a lack of medical personnel to administer parental vaccines, and needle phobia. As an alternative, dissolvable microneedles have recently been studied for their simple and painless application. In this thesis, I present a novel manufacturing process for polymer-based dissolvable microneedles, loaded with virus-like particles vaccines conjugated to TRIO or Sialokinin peptides to target malaria and arboviruses, respectively. We have performed multiple characterization studies …

Optimizing Malaria Control: Granular And Cost-Effective Mosquito Habitat Index In Endemic Areas Through Satellite Imagery, Nur Ainun Daulay, Salwa Rizqina Putri, Arie Wahyu Wijayanto, Ika Yuni Wulansari

Knowledge Engineering and Data Science

Malaria, classified as a tropical disease under the Sustainable Development Goals (SDGs) indicator 3.3, remains a significant global health challenge. In this study, by taking advantage of multiple spectral composite indexes of multisource satellite imagery to capture various geospatial features relevant to the suitability of marsh mosquito habitat, we introduced the Mosquito Habitat Suitability Index (MHSI) to assess potential Anopheles mosquito breeding sites in terms of the vegetation density, water bodies, environment temperature, and humidity in any particular areas. The MHSI integrates the publicly accessible granular level of the normalized difference vegetation index, water index, land surface temperature, and moisture …

Antibiotic Resistance In Urban Stormwater Runoff And Green Stormwater Infrastructure, Kassidy N. O'Malley

Dissertations (1934 -)

Antibiotic resistance in the environment poses significant risks to human health, especially through the transmission of antibiotic resistance genes (ARGs) and antibiotic-resistant bacteria (ARB) via pathways like food and water. Urban stormwater runoff has been identified as a significant contributor to the release and transport of ARGs in the environment but has remained under-researched. This dissertation aimed to address this gap by investigating the fate of ARGs in urban stormwater runoff to guide future stormwater management decisions. Two phases were completed; the first sought to investigate extracellular antibiotic resistance genes (eARGs) in stormwater runoff, and the second focused on quantifying …

Pulsed Electric Fields Sensitize Methicillin-Resistant Staphylococcus Aureus To Antibacterial Therapies And Stimulate Host Immune Responses, Alexandra E. Chittams-Miles

Biomedical Sciences Theses & Dissertations

This research explores the impact of nanosecond pulsed electric fields (nsPEF) on two fronts: their immune stimulatory effects and their potential as a novel strategy to enhance the sensitivity of Methicillin-resistant Staphylococcus aureus (MRSA) to clinically relevant antibiotics. While pulsed electric fields have been reported to have an immune stimulatory effect, the mechanisms responsible for these effects have yet to be determined.

Our investigation addresses the rising concern of MRSA derived skin and soft tissue infections (SSTIs). Consistent with other publications, we found that nsPEF alone cause modest inactivation of planktonic MRSA. We then investigated the effects of nsPEF in …

Existence Of A Nonzero Worst-Case Ach For Short-Term Exposure In Ventilated Indoor Spaces, K. A. Krishnaprasad, ‪Nadim Zgheib, K. Choudhary, M. Y. Ha, C. Y. Choi, K. S. Bang, S. Jang, S. Balachandar

Mechanical Engineering Faculty Publications

A well-ventilated room is essential to reduce the risk of airborne transmission. As such, the scientific community sets minimum limits on ventilation with the idea that increased ventilation reduces pathogen concentration and thus reduces the risk of transmission. In contrast, the upper limit on ventilation is usually determined by human comfort and the need to reduce energy consumption. While average pathogen concentration decreases with increased ventilation, local concentration depends on multiple factors and may not follow the same trend, especially within short exposure times over large separation distances. Here, we show through experiments and high-fidelity simulations the existence of a …

How Individuals’ Opinions Influence Society’S Resistance To Epidemics: An Agent-Based Model Approach, Geonsik Yu, Michael Garee, Mario Ventresca, Yuehwern Yih

Purdue University Libraries Open Access Publishing Fund

Background

Protecting public health from infectious diseases often relies on the cooperation of citizens, especially when self-care interventions are the only viable tools for disease mitigation. Accordingly, social aspects related to public opinion have been studied in the context of the recent COVID-19 pandemic. However, a comprehensive understanding of the effects of opinion-related factors on disease spread still requires further exploration.

Methods

We propose an agent-based simulation framework incorporating opinion dynamics within an epidemic model based on the assumption that mass media channels play a leading role in opinion dynamics. The model simulates how opinions about preventive interventions change over …

Sanitation In Urban Areas May Limit The Spread Of Antimicrobial Resistance Via Flies, Drew Capone, Oliver Cumming, Abeoseh Flemister, Victor Ilevbare, Seth R. Irish, Ishi Keenum, Jackie Knee, Rassul Nala, Joe Brown

Michigan Tech Publications

Synanthropic filth flies are common where sanitation is poor and fecal wastes are accessible to them. These flies have been proposed as mechanical vectors for the localized transport of fecal microbes including antimicrobial resistant (AMR) organisms and associated antimicrobial resistance genes (ARGs), increasing exposure risks. We evaluated whether an onsite sanitation intervention in Maputo, Mozambique reduced the concentration of enteric bacteria and the frequency of detection of ARGs carried by flies collected in household compounds of low-income neighborhoods. Additionally, we assessed the phenotypic resistance profile of Enterobacteriaceae isolates recovered from flies during the pre-intervention phase. After fly enumeration at study …

Composting Reduces The Risks Of Resistome In Beef Cattle Manure At The Transcriptional Level, Yuepeng Sun, Zachery R. Staley, Bryan Woodbury, Jean-Jack Riethoven, Xu Li

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Transcriptomic evidence is needed to determine whether composting is more effective than conventional stockpiling in mitigating the risk of resistome in livestock manure. The objective of this study is to compare composting and stockpiling for their effectiveness in reducing the risk of antibiotic resistance in beef cattle manure. Samples collected from the center and the surface of full-size manure stockpiling and composting piles were subject to metagenomic and metatranscriptomic analyses. While the distinctions in resistome between stockpiled and composted manure were not evident at the DNA level, the advantages of composting over stockpiling were evident at the transcriptomic level in …

Carbon Dots For Drug Delivery And Diagnostic Applications, Sneha Bhat

International Conference on ‘NANOSCIENCE AND NANOTECHNOLOGY'

No abstract provided.

Revolutionizing Healthcare: The Promise Of Microbivores, Clottocytes And Respirocytes In Nanorobotics., Jeyasankari S

International Conference on ‘NANOSCIENCE AND NANOTECHNOLOGY'

No abstract provided.

Utilization Of The System Engineering Design Process To Design And Test A Low-Cost Infectious Aerosol Control Mechanism For Patient Aeromedical Evacuation, Sara Shaghaghi

Theses and Dissertations

The aeromedical evacuation of military patients is a critical component of care for Armed Forces members. The Air Force’s ability to transport patients relies on the technology and systems available. A vital transport responsibility is keeping the patient and medical personnel safe during transport. The historical and legacy systems provide reliable transport mechanisms for the Armed Forces’ patients infected with high-level biological agents, but drawbacks must be considered. This dissertation will discuss the development, conceptual design, and initial evaluation of a new low-cost, litter-mounted patient transport system, Biological-Mitigation in Patient Transport (B-MIPT), using the “V” model of the system engineering …

An Introduction Of Adaptive Training Aid Concepts And Its Application To Accelerated Training For Air Battle Managers, John C. Gillispie

Theses and Dissertations

Over the years, the integration of artificial intelligence (AI) to enable autonomous systems has undergone transformative shifts in the Department of Defense (DoD), revolutionizing capabilities and strategic approaches. To further optimize these advancements, varying levels of autonomy have been introduced across critical military applications, spanning intelligence, surveillance, reconnaissance (ISR), air battle management, and offensive/defensive air operations. As the technological landscape expands, so do the opportunities for autonomy to augment operations through human-agent teaming. Within the Air Force, one notably cognitively demanding role that stands to benefit from these strides is that of the Air Battle Manager (ABM). In support of …

Deploying Field-Capable Genome Sequencing: Human Performance Considerations Under Various Personal Protective Equipment Ensembles, Steven A. Torres

Theses and Dissertations

Field-portable genome sequencing enhances the Department of Defense's strategy for detecting biological threats, particularly in resource-limited environments. While current field-capable detection methods are effective, challenges in identifying emerging threats pose risks to personnel safety. As biotechnological advancements like portable nanopore genome sequencing can advance biological detection capabilities, the viability of conducting laboratory tasks under Mission-Oriented Protective Posture (MOPP) ensembles lacks sufficient data. This research assesses the effectiveness of tools like pipettes, centrifuges, vortexes, and nanopore sequencing within a MOPP ensemble. Novice participants were utilized to explore the extension of this capability, and they completed tasks in three conditions: Fatigues, Laboratory …

Functional Analysis Of The Role Of Extracellular Vesicles In Pathogenesis Of Diabetic Foot Ulcers, Apoorva Jnana, Malatesh Sangappa Devati, Manjunath B Joshi, Thokur Sreepathy Muralli

International Conference on ‘NANOSCIENCE AND NANOTECHNOLOGY'

No abstract provided.

Sustainability Of Simplified Sewer Systems: A Case Study Of Vingunguti Urban Settlement, Dar Es Salaam Region In Tanzania, Neema Kijazi

Tanzania Journal of Engineering and Technology (TJET)

The universal access to safely managed sanitation remains one of the challenges to achieving the UN SDG target 6.2 in unplanned urban settlements including the Vingunguti area in Dar es Salaam City which is high-density populated. In an attempt to address this challenge, a simplified sewer system was piloted as an option for achieving safely managed sanitation in this particular area. The objective of this study was to assess the sustainability of the piloted simplified sewer system for its potentiality to up-scale and replication in areas with similar sanitation challenges in the country. Methodology involved collection of data on performance …

A Two-Stage Based Life Cycle And Principal Component Analysis For Decision Support Of Potential Municipal Solid Wastes Management Scenarios, Case Study Dar Es Salaam, Tanzania, Edwin Richard Ndibalema Dr

Tanzania Journal of Engineering and Technology (TJET)

In most of the urban areas of Tanzania, poor and inadequate solid waste management is a growing environmental and public health concern. Despite an array of solid waste management options, choosing an appropriate one remains a challenge. This study applied a novel two-stage-based approach to assess the environmental impacts and sustainability performance of the 27 scenarios for municipal solid wastes (MSW) management of Dar es Salaam City, Tanzania. In the first stage, the study utilized a life cycle analysis (LCA) to quantify the environmental impacts of MSW scenarios. In the second stage, the study used the principal component analysis (PCA) …

Longitudinal Attacks Against Iterative Data Collection With Local Differential Privacy, Mehmet Emre Gürsoy

Turkish Journal of Electrical Engineering and Computer Sciences

Local differential privacy (LDP) has recently emerged as an accepted standard for privacy-preserving collection of users’ data from smartphones and IoT devices. In many practical scenarios, users’ data needs to be collected repeatedly across multiple iterations. In such cases, although each collection satisfies LDP individually by itself, a longitudinal collection of multiple responses from the same user degrades that user’s privacy. To demonstrate this claim, in this paper, we propose longitudinal attacks against iterative data collection with LDP. We formulate a general Bayesian adversary model, and then individually show the application of this adversary model on six popular LDP protocols: …

Simple Sars-Cov-2 Concentration Methods For Wastewater Surveillance In Low Resource Settings, Mohammad Dehghan Banadaki, Soroosh Torabi, Alexus Lori Rockward, William Strike, Ann Noble, James W. Keck, Scott M. Berry

UK CARES Faculty Publications

Wastewater-based epidemiology (WBE) measures pathogens in wastewater to monitor infectious disease prevalence in communities. Due to the high dilution of pathogens in sewage, a concentration method is often required to achieve reliable biomarker signals. However, most of the current concentration methods rely on expensive equipment and labor-intensive processes, which limits the application of WBE in low-resource settings. Here, we compared the performance of four inexpensive and simple concentration methods to detect SARS-CoV-2 in wastewater samples: Solid Fraction, Porcine Gastric Mucin-conjugated Magnetic Beads, Calcium Flocculation- Citrate Dissolution (CFCD), and Nanotrap® Magnetic Beads (NMBs). The NMBs and CFCD methods yielded the highest …

Mathematical Model Of Oxygen, Nutrient, And Drug Transport In Tuberculosis Granulomas, Meenal Datta, Mccarthy Kennedy, Saeed Siri, Laura Via, James W. Baish, Lei Xu, Veronique Dartois, Clifton Barry, Rakesh Jain

Faculty Journal Articles

Physiological abnormalities in pulmonary granulomas–pathological hallmarks of tuberculosis (TB)–compromise the transport of oxygen, nutrients, and drugs. In prior studies, we demonstrated mathematically and experimentally that hypoxia and necrosis emerge in the granuloma microenvironment (GME) as a direct result of limited oxygen availability. Building on our initial model of avascular oxygen diffusion, here we explore additional aspects of oxy- gen transport, including the roles of granuloma vasculature, transcapillary transport, plasma dilution, and interstitial convection, followed by cellular metabolism. Approximate analytical solutions are provided for oxygen and glucose concentration, interstitial fluid velocity, interstitial fluid pressure, and the thickness of the convective zone. …

Quaternary Ammonia Compounds In Disinfectant Products: Evaluating The Potential For Promoting Antibiotic Resistance And Disrupting Wastewater Treatment Plant Performance, Zihao Lu, Anna K. Mahony, William A. Arnold, Christopher Marshall, Patrick J. Mcnamara

Civil and Environmental Engineering Faculty Research and Publications

Quaternary ammonium compounds (QACs) are a class of compounds that were widely used as disinfectants during the COVID-19 pandemic and continue to be used as disinfecting agents. After consumer usage, QAC concentrations are diluted in wastewater as they enter wastewater treatment plants. At sub-inhibitory concentrations, QACs may have unintended repercussions, including increased antibiotic resistance and inhibition of process performance in wastewater treatment plants. This review first summarizes how QACs inhibit bacteria and then highlights the mechanisms by which QACs can promote antibiotic resistance in general. Reported environmental concentrations of QACs are compared to concentrations that are suspected to impact antibiotic …

Research On Campus Epidemic Evolution Based On Multi-Scale Modeling And Simulation In Microscopic & Microscopic View, Mingwei Hu, Wenjie Yang

Journal of System Simulation

Abstract: High density of population leads to high possibility of cross-infection. It is necessary to focus on campus epidemic prevention and control. Basing on existing studies in macroscopic or microscopic view, this paper proposed a multi-scale means to analyze a short-term evolution of Corona virus disease 2019 (COVID-19) on campus and estimated the efficiency of prevention strategies. Macroscopic model was based on the susceptible-exposed-infections-recovered(SEIR) model, which exported the time curve of the number of asymptomatic patients and symptomatic patients. Microscopic model combined discrete event simulation modeling and agent-based modeling to simulate the behavior of campus students and the state evolution …

Engineering Neurotoxin-Functionalized Exosomes For Targeted Delivery To The Peripheral Nervous System, Mena Asha Krishnan, Olawale A. Alimi, Tianshu Pan, Mitchell Kuss, Zeljka Korade, Guoku Hu, Bo Liu, Bin Duan

Department of Mechanical and Materials Engineering: Faculty Publications

The administration of therapeutics to peripheral nerve tissue is challenging due to the complexities of peripheral neuroanatomy and the limitations imposed by the blood–nerve barrier (BNB). Therefore, there is a pressing need to enhance delivery effectiveness and implement targeted delivery methods. Recently, erythrocyte-derived exosomes (Exos) have gained widespread attention as biocompatible vehicles for therapeutics in clinical applications. However, engineering targeted Exos for the peripheral nervous system (PNS) is still challenging. This study aims to develop a targeted Exo delivery system specifically designed for presynaptic terminals of peripheral nerve tissue. The clostridium neurotoxin, tetanus toxin-C fragment (TTC), was tethered to the …

Computational And Experimental Analysis Of Silica Nanoparticles Under Dc And Ac Electric Fields Using A Self-Induced Back Action Actuated Nanopore Electrophoresis (Sane) Sensor, Homayoun Asadzadeh, Scott Renkes, George Alexandrakis, Minjun Kim, Scott Turpin

Bioengineering Dissertations - Archive

In this dissertation I have studied a novel single-molecule nanosensor that integrates a Solid-State Nanopore (SSNP), milled in silicon nitride with a Double Nanohole (DNH) nanoaperture, milled in gold. This device leverages Self-Induced Back-Action (SIBA) for optical trapping with ssNP electrical sensing and is called the SIBA-Actuated Nanopore Electrophoresis (SANE) sensor. My lab has previously shown that simultaneous acquisition of bimodal optical and direct current (DC) electrical signatures enables more detailed characterization of nanoscopic analytes compared to using electrical data alone, which are typically collected by ssNP sensors. In this work I have first studied by COMSOL Multiphysics simulations the …

Guardians Of The Catheters, Interprofessional Collaboration Reduced Cauti Rates At Phfh, Dawn Doud, Angela Shemwell, Stephanie Brown, Tobi Bryan, Georgia Gauron, Tasha Anisimov, Peter Weitzman, Emma Peterson, Tiffanie Rampley

Articles, Abstracts, and Reports

Abstract Title: A Quality Improvement Project to Reduce CAUTIs on Inpatient Units

Objectives:

1. Participants will be able to understand how interprofessional collaboration contributed to CAUTI reduction on inpatient acute care units.
2. Participants will be able to understand education nurses completed to reduce CAUTI rates on inpatient acute care units.

Purpose/Aims:

To reduce CAUTI rates for adult inpatients admitted to the acute care units: Progressive Care Unit (PCU), Surgical/Orthopedics, Medical/Oncology, and the Intensive Care Unit (ICU).

Background

An increase in CAUTI rates for adults admitted to inpatient units was identified in January of 2024. The CAUTI rate at that time was …