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Biorecognition Ability Of Polysaccharides As Piezo Quartz Biosensors, En Kalmykova, Ca Mgina

Tanzania Journal of Science

Piezoquartz biosensors (PQB) which are analytical devices for recognition of biochemical interactions have recently attracted increasing interest from different researchers such as analysts, immune-chemists, medical doctors, environmentalists, etc. This is due to the advantages of PQB for having high detection sensitivity (at ng and mg level depending on the mass of sorbates or micro-organisms) and the ability to monitor biochemical reactions in turbid or highly coloured liquids (including in biological fluids) in real time. The use of additional markers and preliminary sample preparation are not required in this biorecognition process. This paper reports on the principle of the PQB and …

Ultrasound-Induced Phase Change Of Emulsion Droplets For Targeted Gene And Drug Delivery, James R. Lattin

Theses and Dissertations

This dissertation explores the potential of using perfluorocarbon emulsion droplets to add an ultrasound-sensitive element to drug delivery systems. These emulsion droplets may be induced to vaporize with ultrasound; during the rarefactional phase of an ultrasound wave, the pressure around the droplets may fall below the vapor pressure of the liquid forming the emulsion, providing a thermodynamic potential for vaporization. This ultrasound-induced phase change of the emulsion droplet could release therapeutics attached to the droplet surface or aid in drug delivery due to mechanical effects associated with vaporization and expansion, similar to the ability of cavitating bubbles to aid in …

Towards A Fluid Solid Interaction Model Of A Dynamic Lung, Justin C. Jacobs

Electronic Theses and Dissertations

Because the pulmonary system is a site for both environmental particulate contamination, as well as drug delivery into the body, numerous research groups have focused on precisely understanding its inner-workings. Past research has demonstrated the need to realistically model the lung walls in order to accurately capture the complex airflow profile throughout all of the branches. Since this is paramount to properly replicating particulate transport in the lung, computational fluid dynamics simulations on their own are inadequate, as they cannot account for lung wall dilation. Only by coupling the fluid and solid domains can natural lung behavior can be effectively …

Inactivation Of Bacterial Opportunistic Skin Pathogens By Nonthermal Dc-Operated Afterglow Atmospheric Plasma, L. C. Heller, C. M. Edelblute, A. M. Mattson, X. Hao, J. F. Kolb

Bioelectrics Publications

AIMS: Multidrug-resistant opportunistic pathogens are clinically significant and require the development of new antimicrobial methods. In this study, Acinetobacter baumannii, Pseudomonas aeruginosa and Staphylococcus aureus cells were exposed to atmospheric plasma on agar plates and in vitro on porcine skin for the purpose of testing bacterial inactivation.

METHODS AND RESULTS: Microbial inactivation at varying exposure durations was tested using a nonthermal plasma jet generated with a DC voltage from ambient air. The observed reduction in colony forming units was quantified as log₁₀ reductions.

CONCLUSIONS: Direct plasma exposure significantly inactivated seeded bacterial cells by approx. 6 log_{10 …}

Mitigation Of Radiation Induced Pulmonary Vascular Injury By Delayed Treatment With Captopril, Robert C. Molthen, Qingping Wu, Brian L. Fish, John E. Moulder, Elizabeth R. Jacobs, Meetha M. Medhora

Biomedical Engineering Faculty Research and Publications

Background and Objective: A single dose of 10 Gy radiation to the thorax of rats results in decreased total lung angiotensin-converting enzyme (ACE) activity, pulmonary artery distensibility and distal vascular density while increasing pulmonary vascular resistance (PVR) at 2 months post-exposure. In this study, we evaluate the potential of a renin-angiotensin system (RAS) modulator, the ACE inhibitor captopril, to mitigate this pulmonary vascular damage.

Methods: Rats exposed to 10 Gy thorax only irradiation and age-matched controls were studied 2 months after exposure, during the development of radiation pneumonitis. Rats were treated, either immediately or 2 weeks after radiation exposure, with …

Did Mineral Surface Chemistry And Toxicity Contribute To Evolution Of Microbial Extracellular Polymeric Substances?, Jie Xu, Jay M. Campbell, Nianli Zhang, William J. Hickey, Nita Sahai

College of Polymer Science and Polymer Engineering

Modern ecological niches are teeming with an astonishing diversity of microbial life in biofilms closely associated with mineral surfaces, which highlights the remarkable success of microorganisms in conquering the challenges and capitalizing on the benefits presented by the mineral-water interface. Biofilm formation capability likely evolved on early Earth because biofilms provide crucial cell survival functions. The potential toxicity of mineral surfaces toward cells and the complexities of the mineral-water-cell interface in determining the toxicity mechanisms, however, have not been fully appreciated. Here, we report a previously unrecognized role for extracellular polymeric substances (EPS), which form biofilms in shielding cells against …

Locating And Protecting Facilities Subject To Random Disruptions And Attacks, Hugh Medal

Graduate Theses and Dissertations

Recent events such as the 2011 Tohoku earthquake and tsunami in Japan have revealed the vulnerability of networks such as supply chains to disruptive events. In particular, it has become apparent that the failure of a few elements of an infrastructure system can cause a system-wide disruption. Thus, it is important to learn more about which elements of infrastructure systems are most critical and how to protect an infrastructure system from the effects of a disruption. This dissertation seeks to enhance the understanding of how to design and protect networked infrastructure systems from disruptions by developing new mathematical models and …

The Antimycobacterial Activity Of Hypericum Perforatum Herb And The Effects Of Surfactants, Shujie Shen

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Tuberculosis is a stubborn and lethal infectious disease. The world-wide tuberculosis surveillance reports that the new TB cases ranges from seven to eight million per year and deaths ranges from 1.3 to 1.6 million every year. The treatment of tuberculosis mainly depends on antituberculosis drugs, such as isoniazid, rifampin, pyrazinamide and ethambutol. However, drug-resistance acquisition is an inevitable process during tuberculosis treatment. Today, multi-drug resistant and the extensively drug-resistant TB cases have been widely reported around the world. Thus, the discovery of novel antituberculosis drugs is essential to effective TB treatment. Hypericum perforatum, also called St. John’s Wort, is …

Electrophoretic Screen And Functionalized Microspheres As Antigen Assays, Alejandra Hernandez, Julio Rincon, Thomas Boland

COURI Symposium Abstracts, Summer 2012

Immunoassays are used for medical diagnosis of infectious diseases. People in developing countries usually do not have access to this type of technologies, which makes infectious diseases the main cause of death in these countries. Immunoassay techniques assist the medical field with diagnosing, prognosis and monitoring of analytes present in the human body. This project will develop a portable antigen detection assay built using an electrophoretic screen with functionalized microspheres. A portable electrophoretic immunoassay will permit the reduction of lab materials and the independence of lab installations making it more accessible. This project involves two main steps: The screen modification …

The Design And Fabrication Of A Silicon Membrane For Electrostatic Actuation, Elizabeth L. Brooks

Materials Engineering

An electrostatically actuated silicon membrane was designed and fabricated utilizing a siliconon- insulator wafer. The SOI wafer contains three layers- a 400μm thick “handle” layer of silicon followed by a 2μm thick oxide layer topped with a 20μm thick “device” layer of silicon. The embedded oxide layer acts as an etch-stop during silicon etching, leaving a thin membrane of consistent thickness on the device side. The device dimensions were chosen to provide acceptable membrane deflection predicted by calculations and Finite Element Analysis. A microfabrication process plan was designed to produce thin membranes appropriate for electrostatic actuation. A 700nm layer of …

Comparing Anti-Vegf Antibodies And Aptamers On Paper Microfluidic-Based Platforms, Katherine Noel Clayton

Master's Theses

The field of microfluidics is expanding into what is known as paper microfluidics. This uses a paper platform rather than materials (i.e. PDMS, PMMA) that are commonly used in microfluidics research. Current devices require an expensive manufacturing process and external sources to power the device. Such devices are not practical in low resource environments. As a consequence, it is the goal of this Thesis to develop a three-dimensional, multiplexed assay chip using nitrocellulose membranes. This device comprises of multiple layers of nitrocellulose membranes with defined fluidic channels. The multiple layers are bound together using double backed tape, and imbedded between …

Nitrocellulose Paper Based Microfluidic Platform Development And Surface Functionalization With Anti-Ige Aptamers, Jennifer Guerin Ward

Master's Theses

The purpose of this thesis project was to demonstrate the ability to utilize the capabilities of aptamers so that they may act as capture reagents for paper microfluidic devices. Several characterization experiments were conducted as a precursor before the final experimentation was performed. Paper characterization, manufacturing protocols for printing and heating, as well as 3D chip fabrication were all performed and analyzed. The results confirmed that the control of fluid through a 3D microfluidic device based in nitrocellulose is possible.

For the biochemistry portion of this thesis report, antibodies and aptamers were chosen to react with IgE, an antibody that …

Purification Of Cell-Based Influenza H5n1 Viruses By Liquid Chromatography Technologies, Alan Yung-Chih Hu

Vaccine Technology IV

No abstract provided.

Bacterium-Like Particles As Delivery Vehicles For Multimeric Antigens, Kees Leenhouts

Vaccine Technology IV

No abstract provided.

Systematically Characterizing Adventitious Agent Tests For Biologics, Rebecca Sheets

Vaccine Technology IV

No abstract provided.

Building Pandemic Preparedness Through A Sustainable Enterprise For Influenza Vaccines, Robert Huebner

Vaccine Technology IV

No abstract provided.

The Cmc Challenges In Developing An Oncolytic Immunotherapy, Colin Love

Vaccine Technology IV

No abstract provided.

Conference Program, Engineering Conferences International

Vaccine Technology IV

No abstract provided.

Expectation Of China's Contribution To World Vaccine Development And Supplies: Status, Strategy And International Approach, Li Shi

Vaccine Technology IV

No abstract provided.

The Role Of Public-Private Partnerships In Advancing Vaccine Technologies And Improving Vaccine Effectiveness And Delivery For Developing Countries, Ray Cummings

Vaccine Technology IV

No abstract provided.

Rational Design Of A Fully Synthetic Nanoparticle-Based Vaccine For Smoking Cessatio, Takashi Kishimoto

Vaccine Technology IV

No abstract provided.

111 Years Producing Immunobiologicals: New Challenges, Jorge Kalil

Vaccine Technology IV

No abstract provided.

Point Of Use Water Treatment With Forward Osmosis For Emergency And Population Migration Relief, Ethan L. Butler

Honors Scholar Theses

According to the World Health Organization, 780 million people do not have access to an improved water source. Many of these people are victims of emergencies and/or forced population migration, and suffer from malnutrition and diarrheal illness as well. One promising solution is Hydration Technology Innovation’s (HTI’s) forward osmosis (FO) systems: the HydroWell and Village System. These systems produce clean drink from almost any source water. A systematic evaluation of HTI’s systems was performed. Bench-top testing showed that HTI’s membrane can remove significant concentrations of inorganic contaminants that have been found to be problematic in disaster-prone regions. A cost model …

Technological Aspects Of Molecular Diagnosis Of Bacterial Infectious Diseases, Christine S. Booth

Department of Chemical and Biomolecular Engineering: Dissertations, Theses, and Student Research

The polymerase chain reaction (PCR) is continually growing in its application, particularly in the field of molecular diagnosis of disease from clinical specimens. The main focus has been in the detection and identification of pathogens. However, quantitative PCR is increasingly utilized to determine initial pathogen load. A well-designed PCR protocol is required in all of these instances. Just as importantly, in the context of disease diagnosis; is the design of the sample processing methodology. The ideal method should concentrate the DNA and effectively isolate a high-quality DNA product, free of PCR inhibitors, while also being simple, reproducible and safe.

The …

Aptamer-Based Spr Biosensor For Detection Of Avian Influenza Virus, Hua Bai

Graduate Theses and Dissertations

Rapid and specific detection of avian influenza (AI) virus is urgently needed with the concerns over the outbreaks of highly pathogenic H5N1 avian influenza in animal and human infection. Aptamers are artificial oligonucleic acid that can specifically bind to target molecules. They show comparable affinity for target virus and better thermal stability than monoclonal antibodies. Those advantages make aptamers promising candidates in diagnostic and detection applications. The goal of this research was to use DNA&ndashaptamer as the specific recognition element in a portable surface plasmon resonance (SPR) biosensor for detection of AI H5N1 virus in poultry.

A SPR biosensor was …

The Effect Of Cockpit Noise On The Temporary Threshold Shift Of Cessna 172sp Flight Instructors, Andrew Robert Bellini

Doctoral Dissertations and Master's Theses

The purpose of this thesis was to study the temporary threshold shift of general aviation flight instructors resulting from their working environment. Exposure to noise before a temporary threshold shift completely recovers can cause a permanent threshold shift with no possibility of recovery, resulting in permanent hearing loss. A result showing minimal to no temporary threshold shift would indicate that hearing personal protective equipment is working properly. This study used sound-level measurements, and audiometric testing, together with survey data to determine whether or not flight instructors were at risk for potential hearing impairment due to temporary threshold shift. Independent t …

Engineering Of Polyamidoamine (Pamam) Dendrimers For Gene And Drug Delivery, Quan Yuan

Theses and Dissertations

Dendrimers are a class of polymers with a highly branched, three-dimensional architecture composed of an initiator core, several interior layers of repeating units and multiple surface groups. They have been recognized as the most versatile compositionally and structurally controlled nanoscale building blocks throughout the fields of engineering, materials science, chemistry, and biology, and they have been widely investigated for drug and gene delivery. Polyamidoamine (PAMAM) dendrimers have inherent properties for gene delivery because of their high buffering capacity, polycationic surface and numerous surface groups for biofunctionlization. This dissertation is organized into four independent sections. The first section investigates a series …

Characterization And Manipulation Of Nanorods Via An Applied Magnetic Field, Summer Wu '13

Student Publications & Research

A remote system capable of controlling the motion of magnetic nanorods was created. Two different systems, electromagnets and magnetic stirrers, were used to create continuously changing magnetic fields thus applying a constant torque to make nanorods rotate as “nano stirbars”. The system was tested on three different types of synthesized nanorods: multi-segmented Ni/Au, pure Ni, and pure Au nanorods. Ni and Au were chosen due to the combination of the magnetic properties of Ni and the biocompatible properties of Au. Variables such as length, shape, and geometries of the nanorods were characterized by a scanning electron microscope and an atomic …

Semi-Interpenetrating Nanofiber Scaffolds For Transbuccal Drug Delivery, Donald Aduba Jr.

Theses and Dissertations

The oral buccal mucosa is a promising absorption site for drug administration because it is permeable, highly vascularized and allows ease of administration. Although there are many platforms that have been used for drug delivery, nanofiber scaffolds as a platform for local or systemic drug delivery through the oral mucosa have not been fully explored. In this thesis, we fabricated a biocompatible electrospun gelatin nanofiber scaffold for local drug delivery at the oral mucosa. To stabilize the electrospun gelatin nanofibers and allow non-invasive incorporation of therapeutics into the scaffold, photo-reactive polyethylene-glycol (PEG)-diacrylate was employed to crosslink the scaffold to form …

Science And Society: The Role Of Long-Term Studies In Environmental Stewardship, Charles T. Driscoll, Kathleen F. Lambert, F. Stuart Chapin, David J. Nowake, Thomas A. Spies, Frederick J. Swanson, David B. Kittredge, Clarisse M. Hart

Civil and Environmental Engineering - All Scholarship

Long-term research should play a crucial role in addressing grand challenges in environmental stewardship. We examine the efforts of five Long Term Ecological Research Network sites to enhance policy, management, and conservation decisions for forest ecosystems. In these case studies, we explore the approaches used to inform policy on atmospheric deposition, public land management, land conservation, and urban forestry, including decisionmaker engagement and integration of local knowledge, application of models to analyze the potential consequences of policy and management decisions, and adaptive management to generate new knowledge and incorporate it into decisionmaking. Efforts to enhance the role of long-term research …