Digital Commons Network^™

Cell Bank Characterization And Fermentation Optimization For Production Of Recombinant Heavy Chain C-Terminal Fragment Of Botulinum Neurotoxin Serotype E (Rbonte(HC): Antigen E) By Pichia Pastoris, Jayanta Sinha, Mehmet Inan, Sarah Fanders, Shinichi Taoka, Mark Gouthro, Todd Swanson, Rick Barent, Ardis Barthuli, Bonnie M. Loveless, Leonard A. Smith, Theresa Smith, Ian Henderson, John Ross, Michael M. Meagher

Papers in Biochemical Engineering

A process was developed for production of a candidate vaccine antigen, recombinant C-terminal heavy chain fragment of the botulinum neurotoxin serotype E, rBoNTE(H_c)in Pichia pastoris. P. pastoris strain GS115 was transformed with the rBoNTE(H_c) gene inserted into pHILD4 Escherichia coli—P. pastoris shuttle plasmid. The clone was characterized for genetic stability, copy number, and BoNTE(H_c) sequence. Expression of rBoNTE(H_c) from the Mut⁺ HIS4 clone was confirmed in the shake-flask, prior to developing a fed-batch fermentation process at 5 and 19 L scale. The fermentation process consists of a glycerol growth phase in …

A Dendroclimatic Record Of Paleoclimate Of The Last 10,000 Years, Glacier Bay National Park And Preserve: Progress Understanding Climate Change In Southeast Alaska, Daniel Lawson, Greg Wiles, David Finnegan

United States Army: Publications

Our investigations in 2007 focused on continuing collection and analyses of tree rings from living trees and building a long ring-width series from interstadial wood. These interstadial forests were extensive and their preservation, although discontinuous and fragmented, is remarkable. In situ stumps, still rooted in growth position, and logs held within sediments remain from forests that existed at various times between glaciations over approximately the last 10,000 years.
We have analyzed previous and new collections of ring-width tree-ring series, including a multiple species collection from near Excursion Ridge in the southeast portion of the Park. These tree-ring series have a …

Drug Loading And Release From Polypeptide Multilayer Nanofilms, Yang Zhong

Doctoral Dissertations

Polypeptides, linear macromolecules, are formed from amino acid residues by linkage of peptide bonds. Proteins are polypeptides too, with more complex conformations contributing to specific functionalities. Disulfide bonds are very important to maintain the structure and functions of proteins, which will form between two cysteine (Cys) residues under oxidizing circumstance.

Cys containing polypeptides are designed and synthesized by F-moc (9-Fluorenylmethyloxycarbonyl) chemistry. The number and position of Cys residues can be controlled by amino acid sequences design and following peptide synthesis, which is important to gain insights on the nature of polyelectrolyte multilayer film assembly and stability.

Both commercial and designed …

Mercury Contamination In Forest And Freshwater Ecosystems In The Northeastern United States, Charles T. Driscoll, Young-Ji Han, Celia Y. Chen, David C. Evers, Kathleen Fallon Lambert

Civil and Environmental Engineering - All Scholarship

Eastern North America receives elevated atmospheric mercury deposition from a combination of local, regional, and global sources. Anthropogenic emissions originate largely from electric utilities, incinerators, and industrial processes. The mercury species in these emissions have variable atmospheric residence times, which influence their atmospheric transport and deposition patterns. Forested regions with a prevalence of wetlands and of unproductive surface waters promote high concentrations of mercury in freshwater biota and thus are particularly sensitive to mercury deposition. Through fish consumption, humans and wildlife are exposed to methylmercury, which markedly bioaccumulates up the freshwater food chain. Average mercury concentrations in yellow perch fillets …

Verification Of Pilot-Scale Iron Release Models, Stephen Glatthorn

Electronic Theses and Dissertations

A model for the prediction of color release from a pilot distribution system was created in 2003 by Imran. This model allows prediction of the release of color from aged cast iron and galvanized steel pipes as a function of water quality and hydraulic residence time. Color was used as a surrogate measurement for iron, which exhibited a strong linear correlation. An anomaly of this model was an absence of a term to account for pH, due to the influent water being well stabilized. A new study was completed to evaluate the effectiveness of corrosion inhibitors against traditional adjustment. Two …

Quantification Of Emissions From Lawn And Garden Equipment In Central Florida, Megan Leigh Crum

Electronic Theses and Dissertations

The objective of this study was to evaluate the practical limits of EPA's NONROAD 2005 to accurately simulate Central Florida conditions, especially with regard to lawn and garden equipment. In particular we investigated a NONROAD emission inventory using default inputs and then created a locally specific emission inventory. These emission inventories were prepared for Orange, Osceola, and Seminole county and focused only on the VOC and NOx emissions caused by lawn and garden equipment. The model was manipulated to assess its ability to represent this specific category of nonroad equipment for a given airshed first by running a base case …

Integrated Topologies And Digital Control For Satellite Power Management And Distribution Systems, Hussam Al-Atrash

Electronic Theses and Dissertations

This work is focused on exploring advanced solutions for space power management and distribution (PMAD) systems. As spacecraft power requirements continue to increase, paralleled by the pressures for reducing cost and overall system weight, power electronics engineers will continue to face major redesigns of the space power systems in order to meet such challenges. Front-end PMAD systems, used to interface the solar sources and battery backup to the distribution bus, need to be designed with increased efficiency, reliability, and power density. A new family of integrated single-stage power converter structures is introduced here. This family allows the interface and control …

Reviewing The Effects Of Alternative Fuels, Average Speed And Idling Time On Emissions From Orange County School Bus Fleet, Ali Bayat

Electronic Theses and Dissertations

Orange County, FL has been experiencing ozone concentrations in the past several years which in some cases exceeded the national and state standards. The high concentration of ground level ozone can cause a variety of health problems including chest pain, coughing, throat irritation, and congestion or it can worsen bronchitis, emphysema, and asthma. Other effects include reduction of agricultural crop and commercial forest yields, lower growth and survivability of tree seedlings, and higher susceptibility of plant to diseases, pests and other stresses such as harsh weather. The ozone generation rate is directly related to the ambient concentration of nitrogen oxides …

Network Performance Management Using Application-Centric Key Performance Indicators, Susan Mcgill

Electronic Theses and Dissertations

The Internet and intranets are viewed as capable of supplying "Anything, Anywhere, Anytime" and e-commerce, e-government, e-community, and military C4I are now deploying many and varied applications to serve their needs. Network management is currently centralized in operations centers. To assure customer satisfaction with the network performance they typically plan, configure and monitor the network devices to insure an excess of bandwidth, that is overprovision. If this proves uneconomical or if complex and poorly understood interactions of equipment, protocols and application traffic degrade performance creating customer dissatisfaction, another more application-centric, way of managing the network will be needed. This research …

Learning Human Behavior From Observation For Gaming Applications, Christopher Moriarty

Electronic Theses and Dissertations

The gaming industry has reached a point where improving graphics has only a small effect on how much a player will enjoy a game. One focus has turned to adding more humanlike characteristics into computer game agents. Machine learning techniques are being used scarcely in games, although they do offer powerful means for creating humanlike behaviors in agents. The first person shooter (FPS), Quake 2, is an open source game that offers a multi-agent environment to create game agents (bots) in. This work attempts to combine neural networks with a modeling paradigm known as context based reasoning (CxBR) to create …

Urban Infilling Impacts On Florida Solid Waste Facilities, Ravi Chander Nalamothu

Electronic Theses and Dissertations

Increasing urbanization in the US is leading to development or re-development of lands adjacent to solid waste facilities and these lands are being considered for residential communities and commercial projects. Thus, the potential for nuisance complaints against the pre-existing solid waste facility operations has become an increasing reality. The objective of this study was to develop a methodology to gather scientific and quantifiable data related to potential nuisances caused by landfills to determine setbacks and buffer zones near landfill and transfer station operations. Appropriate recommendations for these setbacks were made from case studies conducted at two landfills in Florida. The …

Fate Of Nitrogen And Phosphorus Species From A Black And Goldtm Nugget Mix In A Laboratoy Column Simulated Septic Tank Drainfiel, Timir Shah

Electronic Theses and Dissertations

The presence of nitrates and phosphorus in ground water is a worldwide problem. A septic tank with drainfield that is conventionally designed does not typically remove nitrogen in the form of nitrates. The main risks are in "Blue baby" syndrome and suspected carcinogenic effect of nitrates on humans and the nutrient enrichment of receiving waters. In some areas nitrate and phosphorus removal are essentially required. Thus the information in this report concentrates on using media in the drainfield for the removal of nitrogen and phosphorus. Extensive work has been conducted in the past few decades in order to find suitable …

A Framework For The Assessment And Analysis Of Multi-Hazardsinduced Risk Resulting From Space Vehicles Operations, Serge Sala-Diakanda

Electronic Theses and Dissertations

With the foreseeable increase in traffic frequency to and from orbit, the safe operation of current and future space vehicles at designated spaceports has become a serious concern. Due to their high explosive energy potential, operating those launch vehicles presents a real risk to: (1) the spaceport infrastructure and personnel, (2) the communities surrounding the spaceport and (3) the flying aircrafts whose routes could be relatively close to spaceport launch and reentry routes. Several computer models aimed at modeling the effects of the different hazards generated by the breakup of such vehicles (e.g., fragmentation of debris, release of toxic gases, …

Biostability In Drinking Water Distribution Systems In A Changing Water Quality Environment Using Corrosion Inhibitors, Bingjie Zhao

Electronic Theses and Dissertations

In this study, the bacterial growth dynamics of 14 pilot drinking water distribution systems were studied in order to observe water quality changes due to corrosion inhibitor addition. Empirical models were developed to quantity the effect of inhibitor type and dose on bacterial growth (biofilm and bulk water). Water and pipe coupon samples were taken and examined during the experiments. The coupons were exposed to drinking water at approximately 20°C for at least 5 weeks to allow the formation of a measurable quasi- steady-state biofilm. Bulk water samples were taken every week. In this study, two simple but practical empirical …

Performance Of Steel Products Coated With Zinc And Zinc Alloys In Pressure Treated Wood, Nahb Research Center, Inc.

American Iron and Steel Institute (AISI) Specifications, Standards, Manuals and Research Reports (1946 - present)

No abstract provided.

Campus Communications Systems: Converging Technologies, The Association For Communications Technology Professionals In Higher Education

ACUTA: Other Publications

This book is a rewrite of Campus Telecommunications Systems: Managing Change, a book that was written by ACUTA in 1995. In the past decade, our industry has experienced a thousand-fold increase in data rates as we migrated from 10 megabit links (10 million bits per second) to 10 gigabit links (10 billion bits per second), we have seen the National Telecommunications Policy completely revamped; we have seen the combination of voice, data, and video onto one network; and we have seen many of our service providers merge into larger corporations able to offer more diverse services. When this book was …

Nebraska Blueprint - Winter 2006-07

Nebraska Blueprint (Student Publication)

Table of Contents:

Good for Livestock, Good for the Earth By Michaela McBride
Ethanol Isn’t the Fuel of the Future Editorial by Aaron Holmberg
Ethanol: The Most Viable Alternative Fuel Editorial By Frank Pribyl
Wind Energy: A Global Perspective By Khoa Chu
UNL Should Embrace Energy- Effi cient Buildings By Leila Knowles and Jeremy Emerson
Biodiesel: A Viable Alternative By Brian Neilson
On the Road Again with BioWillie By Brian Neilson
Attitude Adjustment By Aaron Holmberg

Details And Description Of Operations Research, Paul Savory

Department of Industrial and Management Systems Engineering: Reports

The ambiguous term operations research was coined during World War II. A frequent substitute is management science. Operations research is a scientific approach to analyzing problems and making decisions. It uses mathematics and mathematical modeling on computers to forecast the implications of various choices and zero in on the best alternatives. This content paper provides an overview of operations research. It highlights operations research problems, methods for solving problems, different areas of operations research, and example problems.

Understanding Standing, Christopher Storey

Doctoral Dissertations

Research objectives. Psychophysical acceleration threshold is a tool for detecting deficits in dynamic postural control. Our lab has shown differences in the acceleration threshold among young adults, elderly adults, and elderly adults with diabetes. Electromyography, Semmes-Weinstein monofilaments, and hearing tests investigate the underlying physiological mechanisms for the detriments in postural control. Due to peri-sway perturbations, the motion of a person's sway affects the signal to noise ratio for perturbed stance. Since increases in sway range accompany postural instabilities, sway entrainment will allow us to investigate changes in acceleration threshold at different points in sway. The center of pressure, observed …

Impact Of Zinc Orthophosphate Inhibitor On Distribution System Water Quality, Xiaotao Guan

Electronic Theses and Dissertations

This dissertation consists of four papers concerning impacts of zinc orthophosphate (ZOP) inhibitor on iron, copper and lead release in a changing water quality environment. The mechanism of zinc orthophosphate corrosion inhibition in drinking water municipal and home distribution systems and the role of zinc were investigated. Fourteen pilot distribution systems (PDSs) which were identical and consisted of increments of PVC, lined cast iron, unlined cast iron and galvanized steel pipes were used in this study. Changing quarterly blends of finished ground, surface and desalinated waters were fed into the pilot distribution systems over a one year period. Zinc orthophosphate …

Identifying Useful Variables For Vehicle Braking Using The Adjoint Matrix Approach To The Mahalanobis-Taguchi System, Elizabeth A. Cudney, Kenneth M. Ragsdell, Kioumars Paryani

Engineering Management and Systems Engineering Faculty Research & Creative Works

The Mahalanobis Taguchi System (MTS) is a diagnosis and forecasting method for multivariate data. Mahalanobis distance (MD) is a measure based on correlations between the variables and different patterns that can be identified and analyzed with respect to a base or reference group. MTS is of interest because of its reported accuracy in forecasting small, correlated data sets. This is the type of data that is encountered with consumer vehicle ratings. MTS enables a reduction in dimensionality and the ability to develop a scale based on MD values. MTS identifies a set of useful variables from the complete data set …

Improving The Judicial System To Handle Computer Crime, Gerald V. Post, Albert Kagan

Journal of International Technology and Information Management

This paper asked professionals in the legal system to evaluate the current state and effectiveness of laws to identify and deter computer crime. Responses were evaluated with a formal structural equation model. The results generally show that legal professionals believe potential jurors have minimal knowledge of computer crime issues. More importantly, they also believe that judges have little knowledge or experience. A similar lack of knowledge by defense attorneys indicates that it could be difficult for a person accused of computer related infractions to find adequate representation. On the other hand, more experienced participants do not believe computer laws present …

The Role Of Quantitative Analysis In The Information Security Systems Development Lifecycle, Stephen R. Rosenkranz, Michael E. Busing, Faye P. Teer, Karen A. Forcht

Journal of International Technology and Information Management

Today’s numerous Quantitative Analysis (QA) tools have been successfully utilized to solve business problems in diverse applications. However, the application of QA tools in solving information security problems has been sparse. Devising the means and ways to use QA tools in resolving industry-wide security problems has the potential to yield enormous global economic benefit. The purpose of this paper is to explore the use of QA tools as a means of improving the processes involved in the Information Security Systems Development Lifecycle (SecSDL). Information security professionals use the SecSDL as a guide for formulating a comprehensive information security program. The …

Kentucky Water Resources Research Institute Annual Technical Report Fy 2006, Kentucky Water Resources Research Institute, University Of Kentucky

KWRRI Annual Technical Reports (USGS’s 104b Grant Program)

The 2006 Annual Technical Report for Kentucky consolidates reporting requirements of the Section 104(b) base grant award into a single techincal report that includes: 1) a synopsis of each student research enhancement project conducted during the period, 2) citations for related reports and presentations, 3) a description of information transfer activities, 4) a summary of student support during the reporting period, 5) notable awards and achievements during the year, and 6) publications from prior projects.

Acl Injuries: Do We Know The Mechanisms?, Antonie J. Van Den Bogert, Scott G. Mclean

Mechanical Engineering Faculty Publications

Injuries of the anterior cruciate ligament (ACL) remain a frequent occurrence in many sports activities. Recent research has identified risk factors, and prevention strategies are being developed based on these findings. There is, however, still a limited understanding of the actual injury mechanisms. In this presentation we will review the current knowledge on ACL injury mechanisms, and identify needs and opportunities for further research.

Preparing A Property Statement For A Property Company: The Key Stages., Myles Keaveney

Articles

No abstract provided.

Route Disruption Analysis, Adam Kirk, Mei Chen

Kentucky Transportation Center Research Report

In today’s society, transportation has become a vital component of our economy, our national defense, our emergency response systems, and our day-to-day life. Disruption of major highway systems can have serious social, health, economic, security, and environmental consequences. Disruption of highway systems can result from natural or man-made causes, and it may be intentional or unintentional. Examples of the wide-ranging causes of highway disruption include weather, natural disasters, vehicle crashes, hazardous material spills, barge impacts with bridge piers, and acts of terrorism. Disruption of key transportation chokepoints, such as bridges or tunnels, could have disastrous impacts on Kentucky. With this …

A Simple Laboratory Experiment For The Measurement Of Single Phase Permeability, Ali R. Zand, Yuri Sikorski, Matthew S. Sanders, Homayun K. Navaz

Mechanical Engineering Publications

A simple experiment for measuring single-phase permeability of fully saturated porous medium is introduced. The experiment utilizes radial flow of a non-volatile wetting fluid through a porous medium such as ceramic tile, concrete or sand. The radial position of flow front is measured as a function of time and the collected data are analyzed using Darcy's Law to determine the permeability. In addition, the phenomenon of the multiphase flow through the medium with a broad pore size distribution is demonstrated.

In-Situ Optimized Pwas Phased Arrays For Lamb Wave Structural Health Monitoring, Lingyu Yu, Victor Giurgiutiu

Faculty Publications

No abstract provided.

Dynamics Of Wave-Current Boundary Layers Over Very Rough Boundaries, Marion Bandet-Chavanne

Link Foundation Ocean Engineering and Instrumentation Fellowship Reports

In this study, acoustic profiling techniques are used to resolve the two-dimensional spatial structures of the oscillating boundary layer in a phase-averaged sense over a wave orbital amplitude. The principal objective of this work is to examine the spatial variability of turbulent properties over irregular roughness. Field observations are used to investigate the interaction between bottom roughness, flow structure and turbulent properties within the near-bed flow. The overall focus of the research is to relate wave energy dissipation or friction factor estimates to physical roughness elements and understand the dynamics of such processes in the near-bed flow.