Digital Commons Network^™

Characterization And Manipulation Of Double-Stranded Dna Using Atomic Force Microscopy, Lauren Skartvedt

Mechanical Engineering Undergraduate Honors Theses

An atomic force microscope (AFM) is used to scan high-resolution images on the nano scale. The lambda DNA used for this project are 48,502 base pairs in length and are double-stranded. This project utilizes the NanoSurf Core AFM in order to characterize and manipulate strands of lambda DNA which have been deposited on a mica surface. The deposition process of the DNA on the mica surface was developed by the National Institute of Standards and Technology and the University of Colorado - Boulder. The AFM is used in imaging mode to scan the mica surface to locate the DNA. When …

Sim-To-Real Reinforcement Learning Framework For Autonomous Aerial Leaf Sampling, Ashraful Islam

School of Computing: Dissertations, Theses, and Student Research

Using unmanned aerial systems (UAS) for leaf sampling is contributing to a better understanding of the influence of climate change on plant species, and the dynamics of forest ecology by studying hard-to-reach tree canopies. Currently, multiple skilled operators are required for UAS maneuvering and using the leaf sampling tool. This often limits sampling to only the canopy top or periphery. Sim-to-real reinforcement learning (RL) can be leveraged to tackle challenges in the autonomous operation of aerial leaf sampling in the changing environment of a tree canopy. However, trans- ferring an RL controller that is learned in simulation to real UAS …

Tumor Targeting Gold Nanoparticles For Delivery Of Rna And Dna Oligonucleotide Therapies For Glioblastoma., Nicholas Allen

Electronic Theses and Dissertations

Glioblastoma (GBM) brain tumors are highly aggressive gliomas due to genetic and cellular heterogeneity. Current GBM treatment consists of surgical resection of the tumor combined with radio- or chemo-therapies. While these treatments have increased the life expectancy for GBM patients up to 20 months, they have had little effect on the 5-year survival rate. The complex cellular and genetic composition of the tumor makes current treatments less effective long term. One approach to developing more effective GBM treatments is to customize nanoparticle-based drug delivery systems that can directly target the aberrant gene expression patterns within a particular GBM tumor. Delivery …

The Influence Of Myoblast Implantation On Arteriogenesis In Mice With Diet-Induced Obesity, Rayana Gutierrez

Biomedical Engineering

Peripheral arterial occlusive disease (PAOD) is characterized by atherosclerosis, which is the buildup of plaque, consisting largely of cholesterol, in the arterial walls. This plaque accumulation eventually blocks blood flow to the limbs, causing symptoms such as intermittent claudication and tissue death in cases of critical limb ischemia. The body compensates for the reduced perfusion by enlarging pre-existing bypass arteries, known as collaterals, in a process called arteriogenesis. However, in many cases, collateral networks constructed through arteriogenesis fail to enlarge sufficiently or function effectively in patients. Therefore, the development of a therapeutic intervention specifically targeting this process would offer a …

The Bridge Newsletter Spring 2023, Missouri University Of Science And Technology

The Bridge Newsletter

-Virtual reality in architectural engineering
-Steel Bridge team wins fifth consecutive competition
-S&T Volleyball star ‘jumps’ at research opportunity
-Benavides legacy continues

Engineering Of Microparticle Encapsulated Antioxidant To Mitigate Oxidative Stress In Vitro And In Vivo, Kayla Ney

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

Chronic low back pain (cLBP) is one of the leading causes of years lived with disability in the United States. Current treatments for cLBP have variable results across the patient population, and many patients struggle to find consistent relief. Most current treatments focus on the symptom of pain, not the root cause. In painful degenerated discs, oxidative stress and inflammation function in a vicious cycle and perpetuate degeneration, damage, and pain. Therefore, oxidative stress and inflammation are important targets in addressing the source of pain. This work characterizes an antioxidant, BuOE, encapsulated in a chondroitin sulfate microparticle as a novel …

Engineered Material Systems For Mimicking Tissue And Disease, Margrethe Ruding

McKelvey School of Engineering Graduate Student Theses & Dissertations

This thesis comprises two studies involving design and application of soft material systems. The goal of the first study was to design, fabricate, and characterize hydrogel lattice structures with consistent, controllable, anisotropic mechanical properties. Lattices, based on four types of unit cells (cubic, diamond, vintile, and Weaire-Phelan), were printed using stereolithography (SLA) of polyethylene glycol diacrylate (PEGDA). In order to create structural anisotropy in the lattices, unit cell design files were scaled in one direction by a factor of two in each layer and then printed. The mechanical properties of the scaled lattices were measured in shear and compression and …

Investigating Haloacetonitrile (Han) Formation Potential From Nitrogenous Antibiotics And Agrochemicals, Kazi Shafayet Haider

UNLV Theses, Dissertations, Professional Papers, and Capstones

Disinfection can promote safe drinking water. Chlorine and chloramines are the two most used disinfectants. While these disinfectants are effective in inactivating disease-causing pathogens, they also produce hazardous byproducts when they react with organic compounds (precursors). Nitrogenous disinfection byproducts (N-DBPs) are more toxic than their counterparts. The increased use of nitrogen-based agrochemicals and antibitics has led to their detection in different water sources. The presence of secondary amine and aniline in these compounds can act as a source of reactive nitrogen to form N-DBPs. In addition, the increased concentration of bromide in water systems due to anthropogenic activities can enhance …

Comprehensive Long- And Short- Read Sequencing Approaches To Screen The Mutational Landscape After Therapeutic Crispr-Editing For Duchenne Muscular Dystrophy, Mary Jia, Made Harumi Padmaswari, Landon Burcham, Shilpi Agrawal, Alexis Ivy, Abbey Bryan, Christopher E. Nelson

Biomedical Engineering Undergraduate Honors Theses

Duchenne muscular dystrophy (DMD) is an X-linked muscle wasting disease caused by mutations in the Dmd gene resulting in non-functional dystrophin. Adeno-associated virus (AAV)-mediated delivery of classical CRISPR systems resulting in double-stranded breaks (DSBs) has yielded promise as a curative DMD therapeutic. However, the consequences of AAV-CRISPR require more thorough characterization. PCR-enriched short-read amplicon sequencing at cut sites is the most common method of sequencing CRISPR editing, but PCR bias results in unreliable quantification of on-target edits. We analyze orthogonal long- and short-read sequencing approaches to screen the outcomes of CRISPR technologies at the DNA and RNA-level for DMD. We …

Preferences Toward Augmented Reality Technologies For Pedestrian Navigation, Victoria Alejandra Diaz Mejia

Theses and Dissertations

This research investigates the demand for pedestrian navigation supported by augmented reality (AR) via headset and mobile technologies by evaluating factors specific to each technology and the decision makers. Recent advances in digital engineering systems, e.g., digital twins and smart cities, are enabling new services like AR-supported wayfinding for pedestrians. Still, it is unknown whether American pedestrians would use AR, the type devices and feature that add value to their navigation, the demographic profiles more inclined to AR wayfinding, and the sensitivity of such demand to software and hardware cost. As the first investigation addressing the demand for AR pedestrian …

Characterizing Endothelial Damage During Ischemic Stroke Treatment With 3d Cerebrovascular Live Cellular And Finite Element Models, Ana Regina Martinez Dehesa

Theses and Dissertations

Stent retriever mechanical thrombectomy (SRMT) is part of the clinical standard-of-care for acute ischemic stroke (AIS) patients with large vessel occlusion (LVO). Endothelial injury (EI) is widely associated with SRMT. Studies report finding EI markers in over 50% of retrieved clots, suggesting the frequency of EI in MT patients is rather common. The mechanism of EI and its contribution to MT failure and clinical outcomes remain poorly understood because of a lack of techniques to observe EI and study it in vitro. This study aims to investigate the mechanisms of EI induced by SRMT, as well as the effect stent …

Characterization Of Lower Rio Grande Valley Watershed, Abdulkabir O. Aduragba

Theses and Dissertations

The Lower Laguna Madre (LLM) is considered impaired because of the high concentration of bacteria and low level of dissolved oxygen (DO). LLM receives freshwater from the Lower Rio Grande Valley (LRGV) watershed. In other to understand the impairment, LRGV watershed is being studied by relying on water quality data of the contributing drainages and State resource geographic data to identify watershed boundary and pollutant sources.

The study on the North and Central LRGV watershed shows some correlation between the concentration of E. coli/Bacteria, Ammonia, Total Kjeldahl Nitrogen, Total Phosphorus, Nitrate and Nitrite, Chlorophyll-a and the sources of pollutant in …

Developing Executable Digital Models With Model-Based Systems Engineering – An Unmanned Aerial Vehicle Surveillance Scenario Example, Viviana Guadalupe Lopez

Theses and Dissertations

There is an increase in complexity in modern systems that causes inconsistencies in the iterative exchange loops of the system design process and in turn, demands greater quality of system organization and optimization techniques. A recent transition from document-centric systems engineering to Model-Based Systems Engineering (MBSE) is being documented in literature from various industries to address these issues. This study aims to investigate how MBSE can be used as a starting point in developing digital twins (DT). Specifically, the adoption of MBSE for realizing DT has been investigated, resulting in various literature reviews that indicate the most prevalent methodologies and …

Wave Excited Mass-Spring-Damper System For A Self-Recharging Autonomous Underwater Vehicle, Mahmodul Hasan Maheen

Theses and Dissertations

Autonomous underwater vehicles' (AUVs) endurance is constrained by the lifetime of their batteries and the distance that tether wires can traverse. Solving the endurance problem of AUV using the enormous potential of ocean wave energy is the motivation behind this thesis. The objective of this research is to model a mass-spring-damper system to emulate the permanent magnet linear generator (PMLG) of a self-recharging AUV and identify its energy absorption capability through numerical simulation and experimental testing. The research activities started with modeling and fabricating a 1:5 scale model. The scaling was done by comparing the most common AUV size of …

Procedural City Generation With Combined Architectures For Real-Time Visualization, Griffin Poyck

All Theses

The work and research of this paper sought to build upon traditional city generation and simulation in creating a tool that both realistically simulates cities and their prominent features and also creates aesthetic and artistically rich cities using assets that combine several contemporary or near contemporary architectural styles. The major city features simulated are the surrounding terrain, road networks, individual buildings, and building placement. The tools used to both create and integrate these features were created in Houdini with Unreal Engine 5 as the intended final destination. This research was influenced by the city, town, and road networking of Ghost …

Xeno-Free Biomimetic Ecm Model For Investigation Of Matrix Properties On Astrocyte Cell Response, Bayan Saleh

Theses and Dissertations

Astrocytes are crucial in preserving the structural and functional integrity of the central nervous system (CNS). They participate in several physiological processes, such as the uptake of neurotransmitters, control of extracellular ion concentration, and preservation of the blood-brain barrier. Recent research has raised the possibility that astrocytes are also involved in the onset and development of Alzheimer's disease (AD). While the precise mechanisms underlying the development and progression of AD are not fully understood, recent research has suggested that age-related changes in the brain extracellular matrix (ECM) properties may play a critical role in the progression of the disease. Investigating …

Evaluation Of Human Mobility From Crowd-Sourced Cellular Data, Sasa Knezevic

Theses and Dissertations

Understanding patterns of human mobility plays a vital role in urban planning, traffic management, disease control, environmental impact evaluation, provisioning of services by both public and private enterprise and other areas of human activity. In this thesis, the human mobility patterns are studied using crowd sourced cellular data. The study characterizes human mobility through a meaningful set of mobility parameters. Each parameter is clearly defined, extracted from the available data, and described using statistical modeling tools. The parameters are evaluated at different time scales. It has been determined that despite its apparent randomness on the individual level, human mobility on …

A Step-By-Step Guide To Identify The Most Reliable Inventory Management System For Small Businesses Using Discrete Event Simulation And Pros And Cons Analysis: A Case Study At Camid-Florida Tech, Djalma Batista Oliveira Filho

Theses and Dissertations

According to the Department of Labor Statistics (2022), 20.6% of small businesses fail in their first year of operation, 42.6% fail after five years, and over 62.6% fail by their tenth year. One factor that can affect continued viability is the lack of knowledge and experience among small and medium-sized firms in inventory management. Despite the fact that inventory control is one of these businesses' most ignored management responsibilities, the expense of procuring inventory often accounts for a considerable portion of their budget. Due to poor inventory management or an inability to appropriately supervise their inventory, small firms may have …

Rattus Norvegicus As A Biological Detector Of Clandestine Remains And The Use Of Ultrasonic Vocalizations As A Locating Mechanism, Gabrielle M. Johnston

Master's Theses

In investigations, locating missing persons and clandestine remains are imperative. One way that first responder and police agencies can search for the remains is by using cadaver dogs as biological detectors. Cadaver dogs are typically used due to their olfactory sensitivity and ability to detect low concentrations of volatile organic compounds produced by biological remains. Cadaver dogs are typically chosen for their stamina, agility, and olfactory sensitivity. However, what is not taken into account often is the size of the animal and the expense of maintaining and training the animal. Cadaver dogs are typically large breeds that cannot fit in …

Measuring Plume Concentration Variations Under Neutral Atmospheric Conditions, Daniel Williams

Chemical Engineering Undergraduate Honors Theses

Hazardous gases are increasingly becoming a large safety concern as the world continues to grow and industrialize. The modeling of when hazardous gases breach containment is an extremely important objective of consequence assessment planning and response. To improve the understanding of the variability inherent in passive gas releases, a test program to demonstrate the variability of gas concentration measurements at different location in a passive gas plume released from an area source in the ultra-low speed wind tunnel at the Chemical Hazards Research Center. A Flame Ionization Detector was used to measure the concentration of the source cloud. Methane was …

Greenhouse Gas Emissions From Milk Production In The Us, Cailey Cooper

Chemical Engineering Undergraduate Honors Theses

Life cycle assessment is performed to determine the environmental impacts of the life cycle of a product. In this study, the greenhouse gas emissions from milk production in the United States were calculated using a cradle-to-gate analysis. The dairy industry has a noticeable impact on the total greenhouse gas emissions in the US. Performing life cycle assessment provides information about the main emission sources in the life cycle which can spark innovative changes to reduce these emissions. The life cycle assessment was performed using the openLCA software and ReCiPe impact assessment methodology. The results indicated that 1.28 kg of carbon …

Comparing Firocoxib And Meloxicam In The Application Of Microneedle Patch For Transdermal Drug Delivery, Ruohan Li

Chemical Engineering Undergraduate Honors Theses

This thesis compares the performance of meloxicam and firocoxib in the aspects of its physical characteristic, chemical compositions, and in-vitro performances for transdermal pain management microneedle patches on farm animals. The microneedle patches are composed of polyvinyl alcohol (PVA), type I collagen (COL), and chitosan (CHI) as base material that carries NSAIDs to achieve therapeutic purposes. Scanning electron microscopy (SEM) was utilized to observe the morphological and physical characteristics of the microneedle patches. Both meloxicam and firocoxib microneedle patches were successfully prepared using the methodology, with organized microneedle distribution and sizing. And Fourier transform infrared spectroscopy (FTIR) confirmed the chemical …

A Teaching Unit Plan For Introducing Engineering In 1st-2nd Grade Classrooms, Claudia Smith

Chemical Engineering Undergraduate Honors Theses

The field of engineering, and specifically chemical engineering, is projected to increase significantly over the next 10 years. Engineering programs require students to have a strong foundation of math and science. Students in the United States are often underprepared to enter these programs, and the majority of high school students are not interested in pursuing a career in the STEM field at all. For this project, a unit teaching plan was created to introduce students to the field of engineering, providing a foundation for their upper-level STEM classes, and encouraging them to consider engineering as a career in the future. …

Human Suspicious Activity Detection, Nilamben Bhuva

Electronic Theses, Projects, and Dissertations

The detection of suspicious human activity is a crucial aspect of ensuring public safety and security. The aim is to identify suspicious behavior. To accomplish this, we employ the LRCN, a long-term recurrent convolutional network, to detect anomalous activity. It is important to consider the temporal data of the video when classifying suspicious behavior, and the framework uses a combination of CNNs and RNNs to analyze video frames and extract relevant features. The key milestones of this project include conducting research, collecting and pre-processing data, designing and training the model, and evaluating its performance. The resulting detection system can accurately …

Trees Recommendation In Agroforestry Ecosystem Using Nlp, Omkar Sawant

Electronic Theses, Projects, and Dissertations

Agroforestry farming is one of the challenging sectors to grow the crops or farming or variety of trees from the ancient days due to erosion and desertification. This Culminating Experience Project explored how recommendation system can be developed and used in agroforestry. The research questions are Q1. What methods can be used to improve the accuracy and reliability of soil-based agroforestry tree species recommendation systems? Q2. How can agroforestry tree species recommendation systems be tailored to the needs of different stakeholders, such as smallholder farmers or agribusinesses? Q3. What will be the top three, tree recommendations using natural language processing …

Beyond Just Money Transactions: Redesigning Digital Peer-To-Peer Payments For Social Connections, Lingyuan Li

All Dissertations

Financial activities, such as the exchange of money between individuals, have long been considered a crucial aspect of how people build and maintain their interpersonal relationships (i.e., a strong, deep, or close association/acquaintance between two or more people) with individuals they know because money is a sensitive social construct. In particular, over the past decade, how to conduct, manage, and experience money exchanges and processes between individuals has been dramatically transformed due to the increasing popularity of digital peer-to-peer (P2P) payment services (i.e., performing one to one online money transactions via a digital device). In this sense, digital P2P payments …

3d Printing With Photopolymerizable Polyester Resins For Resorbable Medical Device Applications, Mathew Murphy Stanford

All Dissertations

In the past decade, the healthcare industry has seen a significant increase in the use of additive manufacturing (AM or “3D printing”) with subsequent improvement in clinical outcomes.As an exceptional AM technology, vat-photopolymerization (VP), often called stereolithography, can create complex structures and has thus been adopted for a range of biomedical applications including surgical guides, temporary implants, and resorbable tissue scaffolds.However, limitations remain in the availability of photopolymerizable resin materials with appropriate mechanical performance, biodegradability, and biocompatibility for application to resorbable medical devices.

The objective of this work was to employ novel photopolymerizable polyester-based macromers in the development of resorbable …

An Integrated Experimental And Modeling Approach To Design Rotating Algae Biofilm Reactors (Rabrs) Via Optimizing Algae Biofilm Productivity, Nutrient Recovery, And Energy Efficiency, Gerald Benjamin Jones

All Graduate Plan B and other Reports, Spring 1920 to Spring 2023

Microalgae biofilms have been demonstrated to recover nutrients from wastewater and serve as biomass feedstock for bioproducts. However, there is a need to develop a platform to quantitatively describe microalgae biofilm production, which can provide guidance and insights for improving biomass areal productivity and nutrient uptake efficiency. This paper proposes a unified experimental and theoretical framework to investigate algae biofilm growth on a rotating algae biofilm reactor (RABR). The experimental laboratory setups are used to conduct controlled experiments on testing environmental and operational factors for RABRs. We propose a differential-integral equation-based mathematical model for microalgae biofilm cultivation guided by laboratory …

Near-Optimal Control Of A Quadcopter Using Reinforcement Learning, Alberto Velazquez-Estrada

Theses and Dissertations

This paper presents a novel control method for quadcopters that achieves near-optimal tracking control for input-affine nonlinear quadcopter dynamics. The method uses a reinforcement learning algorithm called Single Network Adaptive Critics (SNAC), which approximates a solution to the discrete-time Hamilton-Jacobi-Bellman (DT-HJB) equation using a single neural network trained offline. The control method involves two SNAC controllers, with the outer loop controlling the linear position and velocities (position control) and the inner loop controlling the angular position and velocities (attitude control). The resulting quadcopter controller provides optimal feedback control and tracks a trajectory for an infinite-horizon, and it is compared with …

Blood Flow Regulates Atherosclerosis Progression And Regression, Morgan A. Schake

Department of Mechanical and Materials Engineering: Dissertations, Theses, and Student Research

Atherosclerosis is the most prevalent pathology of cardiovascular disease with no known cure. Despite the many systemic risk factors for atherosclerosis, plaques do not form randomly in the vasculature. Instead, they form around bifurcations and the inner curvature of highly curving arterial segments that contain so-called disturbed blood flow that is low in magnitude and multidirectional over the cardiac cycle. Conversely, straight, non-bifurcated arterial segments that contain moderate-to-high and unidirectional (i.e., normal) blood flow are protected from plaque development. Thus, blood flow is a key regulator of atherosclerosis that may be able to be leveraged to develop new therapeutics. Towards …